Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A1 F28 I1 R2
|
328 |
63.0 |
4687567 |
98.0% |
4593815 |
65.2 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
annotation |
gene |
description |
| RA |
W3110S.gb |
2,426,403 |
G→A |
G113R (GGG→AGG) |
yfcG → |
predicted glutathione S‑transferase |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | W3110S.gb | 2,426,403 | 0 | G | A | 100.0%
| 108.9
/ NA
| 38 | G113R (GGG→AGG) | yfcG | predicted glutathione S‑transferase |
| Reads supporting (aligned to +/- strand): ref base G (0/0); new base A (30/8); total (30/8) |
AGCGCGCCGCCACATTACAGTGGTTATTCTGGCAGGTAGGCGGACTGGGGCCGATGCTTGGGCAAAATCATCATTTTAATCACGCAGCCCCCCAAACCATTCCTTACGCTATTGAACGTTATCAGG > W3110S.gb/2426344‑2426469
|
aGCGCGCCGCCACATTACAGTGGTTATTCTGGCAGGTAGGCGGACTGGGGCCGATGCTTAGGCAAAatcat > 1:3816588/1‑71 (MQ=255)
aGCGCGCCGCCACATTACAGTGGTTATTCTGGCAGGTAGGCGGACTGGGGCCGATGCTTAGGCAAAatcat > 1:4377599/1‑71 (MQ=255)
aGCGCGCCGCCACATTACAGTGGTTATTCTGGCAGGTAGGCGGACTGGGGCCGATGCTTAGGCAAAatcat < 1:197728/71‑1 (MQ=255)
aGCGCGCCGCCACATTACAGTGGTTATTCTGGCAGGTAGGCGGACTGGGGCCGATGCTTAGGCAAAatcat > 1:546548/1‑71 (MQ=255)
cgcgcCGCCACATTACAGTGGTTATTCTGGCAGGTAGGCGGACTGGGGCCGATGCTTAGGCAAAAtcatca < 1:2912821/71‑1 (MQ=255)
ccgccACATTACAGTGGTTATTGTGGCAGGTAGGCGGACTGGGGCCGATGCTTAGGCAAAATCATCAtttt < 1:383138/71‑1 (MQ=255)
ccACATTACAGTGGTTATTCTGGCAGGTAGGCGGACTGGGGCCGATGCTTAGGCAAAATCATCATTTTAAt < 1:1946874/71‑1 (MQ=255)
aCAGTGGTTATTCTGGCAGGTAGGCGGACTGGGGCCGATGCTTAGGCAAAATCATCATTTTAATCa > 1:738943/1‑66 (MQ=255)
aCAGTGGTTATTCTGGCAGGTAGGCGGACTGGGGCCGATGCTTAGGCAAAATCATCATTTTAATCa > 1:4659866/1‑66 (MQ=255)
aCAGTGGTTATTCTGGCAGGTAGGCGGACTGGGGCCGATGCTTAGGCAAAATCATCATTTTAATCACGCAg > 1:1910756/1‑71 (MQ=255)
aCAGTGGTTATTCTGGCAGGTAGGCGGACTGGGGCCGATGCTTAGGCAAAATCATCATTTTAATCACGCAg > 1:4650506/1‑71 (MQ=255)
aCAGTGGTTATTCTGGCAGGTAGGCGGACTGGGGCCGATGCTTAGGCAAAATCATCATTTTAATCACGCAg > 1:1188799/1‑71 (MQ=255)
aCAGTGGTTATTCTGGCAGGTAGGCGGACTGGGGCCGATGCTTAGGCAAAATCATCATTTTAATCACGCAg > 1:4543550/1‑71 (MQ=255)
aCAGTGGTTATTCTGGCAGGTAGGCGGACTGGGGCCGATGCTTAGGCAAAATCATCATTTTAATCACGCAg > 1:3841156/1‑71 (MQ=255)
aCAGTGGTTATTCTGGCAGGTAGGCGGACTGGGGCCGATGCTTAGGCAAAATCATCATTTTAATCACGCAg > 1:114474/1‑71 (MQ=255)
gTGGTTATTCTGGCAGGTAGGCGGACTGGGGCCGATGCTTAGGCAAAATCATCATTTTAATCACGCAGccc > 1:1172174/1‑71 (MQ=255)
aTTCTGGCAGGTAGGCGGACTGGGGCCGATGCTTAGGCAAAATCATCATTTTAATCACGCAGCCCCCCaaa > 1:2712121/1‑71 (MQ=255)
aTTCTGGCAGGTAGGCGGACTGGGGCCGATGCTTAGGCAAAATCATCATTTTAATCACGCAGCCCCCCaaa > 1:2997424/1‑71 (MQ=255)
aTTCTGGCAGGTAGGCGGACTGGGGCCGATGCTTAGGCAAAATCATCATTTTAATCACGCAGCCCCCCaaa > 1:4610049/1‑71 (MQ=255)
gCAGGTAGGCGGACTGGGGCCGATGCTTAGGCAAAATCATCATTTTAATCACGCAGCCCCCCAAACCa < 1:4508239/68‑1 (MQ=255)
tAGGCGGACTGGGGCCGATGCTTAGGCAAAATCATCATTTTAATCACGCAGCCCCCCAAACCATTCCTTAc > 1:1521419/1‑71 (MQ=255)
aGGCGGACTGGGGCCGATGCTTAGGCAAAATCATCATTTTAATCACGCAGCCCCCCAAACCATTCCTTACg > 1:1099393/1‑71 (MQ=255)
aGGCGGACTGGGGCCGATGCTTAGGCAAAATCATCATTTTAATCACGCAGCCCCCCAAACCATTCCTTACg > 1:3726228/1‑71 (MQ=255)
aGGCGGACTGGGGCCGATGCTTAGGCAAAATCATCATTTTAATCACGCAGCCCCCCAAACCATTCCTTACg > 1:1866194/1‑71 (MQ=255)
aGGCGGACTGGGGCCGATGCTTAGGCAAAATCATCATTTTAATCACGCAGCCCCCCAAACCATTCCTTACg > 1:1066417/1‑71 (MQ=255)
ggCGGACTGGGGCCGATGCTTAGGCAAAATCATCATTTTAATCACGCAGCCCCCCAAACCATTCCTTACGc < 1:4574421/71‑1 (MQ=255)
cGGACTGGGGCCGATGCTTAGGCAAAATCATCATTTTAATCACGc > 1:4094466/1‑45 (MQ=255)
tcTGGGGCCGATGCTTAGGCAAAATCATCATTTTAATCACGCAGCCCCCCAAACCATTCCTTACGCt < 1:3467883/66‑1 (MQ=255)
cTGGGGCCGATGCTTAGGCAAAATCATCATTTTAATCACGCAGcc > 1:1675466/1‑45 (MQ=255)
cTGGGGCCGATGCTTAGGCAAAATCATCATTTTAATCACGCAGCCCCCCAAACCATTCCTTACGCTAtt > 1:4211200/1‑69 (MQ=255)
gggCCGATGCTTAGGCAAAATCATCATTTTAATCACGCAGCCCCCCAAACCATTCCt > 1:4218685/1‑57 (MQ=255)
gCCGATGCTTAGGCAAAATCATCATTTTAATCACGCATCCCCCCAAACCATTCCTTACGCTATTGAACGtt < 1:3674273/71‑1 (MQ=255)
gCTTAGGCAAAATCATCATTTTAATCACGCCGCCCCCCAAACCATTCCTTACGCTATTGAACGTTATCAgg > 1:4008348/1‑71 (MQ=255)
gCTTAGGCAAAATCATCATTTTAATCACGCAGCCCCCCAAACCATTCCTTACGCTATTGAACGTTATCAgg > 1:3867118/1‑71 (MQ=255)
gCTTAGGCAAAATCATCATTTTAATCACGCAGCCCCCCAAACCATTCCTTACGCTATTGAACGTTATCAgg > 1:3688499/1‑71 (MQ=255)
gCTTAGGCAAAATCATCATTTTAATCACGCAGCCCCCCAAACCATTCCTTACGCTATTGAACGTTATCAgg > 1:988405/1‑71 (MQ=255)
gCTTAGGCAAAATCATCATTTTAATAACGCAGCCCCCCAAACCATTCCTTACGCTATTGAACGTTATCAgg > 1:2672125/1‑71 (MQ=255)
cTTAGGCAAAATCATCATTTTAATCACGCAGCCCCCCAAACCATTCCTTACGCTATTGAACGTTa > 1:3715255/1‑65 (MQ=255)
|
AGCGCGCCGCCACATTACAGTGGTTATTCTGGCAGGTAGGCGGACTGGGGCCGATGCTTGGGCAAAATCATCATTTTAATCACGCAGCCCCCCAAACCATTCCTTACGCTATTGAACGTTATCAGG > W3110S.gb/2426344‑2426469
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
