Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A1 F8 I0 R1
|
311 |
50.1 |
4085234 |
95.9% |
3917739 |
60.7 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
freq |
annotation |
gene |
description |
| RA |
W3110S.gb |
2,426,403 |
G→A |
29.7% |
G113R (GGG→AGG) |
yfcG → |
predicted glutathione S‑transferase |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | W3110S.gb | 2,426,403 | 0 | G | A | 29.7%
| 51.9
/ 26.3
| 37 | G113R (GGG→AGG) | yfcG | predicted glutathione S‑transferase |
| Reads supporting (aligned to +/- strand): ref base G (10/16); new base A (3/8); total (13/24) |
| Fisher's exact test for biased strand distribution p-value = 7.11e-01 |
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 3.26e-01 |
CGTGAGCGCGCCGCCACATTACAGTGGTTATTCTGGCAGGTAGGCGGACTGGGGCCGATGCTTGGGCAAAATCATCATTTTAATCACGCAGCCCCCCAAACCATTCCTTACGCTATTGAACGTT > W3110S.gb/2426340‑2426463
|
cgTGAGCGCGCCGCCACATTACAGTGGTTATTCTGGCAGGTAGGCGGACTGGGGCCGATGCTTGGGCaaaa > 1:3838358/1‑71 (MQ=255)
cgcgcCGCCACATTACAGTGGTTATTCTGGCAGGTAGGCGGACTGGGGCCGATGCTTGGGCAAAAtcatca < 1:3584794/71‑1 (MQ=255)
acaTTACAGTGGTTATTCTGGCAGGTAGGCGGACTGTGGCCGATGCTTGGGCAAAATCATCATTTTAATCa > 1:1422128/1‑71 (MQ=255)
caTTACAGTGGTTATTCTGGCAGGTAGGCGGACTGGGGCCGATGCTTAGGCAAAatcat < 1:1212634/59‑1 (MQ=255)
caTTACAGTGGTTATTCTGGCAGGTAGGCGGACTGGGGCCGATGCTTAGGCAAAatcat < 1:3678271/59‑1 (MQ=255)
caTTACAGTGGTTATTCTGGCAGGTAGGCGGACTGGGGCCGATGCTTAGGCAAAatcat < 1:2147586/59‑1 (MQ=255)
aTTACAGTGGTTATTCTGGCAGGTAGGCGGACTGGGGCCGATGCTTGGGCAAAATCATCATTTTAATCACg < 1:556879/71‑1 (MQ=255)
ttACAGTGGTTATTCTGGCAGGTAGGCGGACTGGGGCCGATGCTTGGGCAAAATCATCATTTTAATCACg < 1:1662117/70‑1 (MQ=255)
ttACAGTGGTTATTCTGGCAGGTAGGCGGACTGGGGCCGATGCTTGGGCAAAATCATCATTTTAATCACg < 1:2891338/70‑1 (MQ=255)
ttACAGTGGTTATTCTGGCAGGTAGGCGGACTGGGGCCGATGCTTGGGCAAAATCATCATTTTAATCACg < 1:2186332/70‑1 (MQ=255)
ttACAGTGGTTATTCTGGCAGGTAGGCGGACTGGGGCCGATGCTTGGGCAAAATCATCATTTTAATCACGc < 1:2775449/71‑1 (MQ=255)
ttACAGTGGTTATTCTGGCAGGTAGGCGGACTGGGGCCGATGCTTGGGCAAAATCATCATTTTAATCACGc < 1:3813717/71‑1 (MQ=255)
aCAGTGGTTATTCTGGCAGGTAGGCGGACTGGGGCCGATGCTTGGGCAAAATCATCATTTTAATCAc > 1:936419/1‑67 (MQ=255)
aCAGTGGTTATTCTGGCAGGTAGGCGGACTGGGGCCGATGCTTAGGCAAAATCATCATTTTAATCa > 1:1552744/1‑66 (MQ=255)
gTTATTCTGGCAGGTAGGCGGACTGGGGCCGATGCTTGGGCAAAATCATCATTTTAATCACg < 1:3469269/62‑1 (MQ=255)
aTTCTGGCAGGTAGGCGGACTGGGGCCGATGCTTGGGCAAAATCATCATTTTAATCACGCa > 1:2100114/1‑61 (MQ=255)
aTTCTGGCAGGTAGGCGGACTGGGGCCGATGCTTGGGCAAAATCATCATTTTAATCACGCa > 1:1761570/1‑61 (MQ=255)
cTGGCAGGTAGGCGGACTGGGGCCGATGCTTGGGCAAAATCATCA‑TTTGATCACGCAGCCCCCCAAACCa < 1:2337333/70‑1 (MQ=255)
aGGTAGGCGGACTGGGGCCGATGCTTAGGCAAAATCATCATTTTAATCACGCAGCCCCCCAAACCATTCCt < 1:2289056/71‑1 (MQ=255)
aGGTAGGCGGACTGGGGCCGATGCTTAGGCAAAATCATCATTTTAATCACGCAGCCCCCCAAACCATTCCt < 1:803593/71‑1 (MQ=255)
aGGTAGGCGGACTGGGGCCGATGCTTAGGCAAAATCATCATTTTAATCACGCAGCCCCCCAAACCATTCCt < 1:4059040/71‑1 (MQ=255)
aGGTAGGCGGACTGGGGCCGATGCTTAGGCAAAATCATCATTTTAATCACGCAGCCCCCCAAACCATTCCt < 1:1732027/71‑1 (MQ=255)
gTAGGCGGACTGGGGCCGATGCTTGGGCAAAATCATCAtttt < 1:2080367/42‑1 (MQ=255)
gTAGGCGGACTGGGGCCGATGCTTGGGCAAAATCATCAtttt < 1:1753479/42‑1 (MQ=255)
gTAGGCGGACTGGGGCCGATGCTTGGGCAAAATCATCAtttt < 1:1998405/42‑1 (MQ=255)
tAGGCGGACTGGGGCCGATGCTTAGGCAAAATCATCATTTTAATCACGCAGCCCCCCAAACCATTCCTTAc > 1:2463685/1‑71 (MQ=255)
aGGCGGACTGGGGCCGATGCTTGGGCAAAATCATCATTTTAATCACg > 1:1185060/1‑47 (MQ=255)
aGGCGGACTGGGGCCGATGCTTGGGCAAAATCATCATTTTAATCACGCAGCCCCCCAAACCATTCCTTAc > 1:3760741/1‑70 (MQ=255)
cGGACTGGGGCCGATGCTTAGGCAAAATCATCATTTTAATCACGCAGCCCCCCAAACCATTcc > 1:577548/1‑63 (MQ=255)
ggACTGGGGCCGATGCTTGGGCAAAATCATCTTTTTAATCACGCAGCCCCCAAAACCATTCCTTACGCTAt > 1:3228164/1‑71 (MQ=255)
ggACTGGGGCCGATGCTTGGGCAAAATCATCATTTTAATCACGCAGCCCCCCAAACCATTCCTTACGCTAt > 1:3845832/1‑71 (MQ=255)
ggACTGGGGCCGATGCTTGGGCAAAATCATCATTTTAATCACGCAGCCCCCCAAACCATTCCTTACGCTAt > 1:1386256/1‑71 (MQ=255)
cgCCGATGCTTGGGCAAAATCATCATTTTAATCACGCAGCCCCCCAAACCATTCCTTACGCTATTGAACGt < 1:3932028/70‑1 (MQ=255)
gCCGATGCTTGGGCAAAATCATCATTTTAATCACGCAGCCCCCCAAACCATTCCTTACGCTATTGAACGtt < 1:1751547/71‑1 (MQ=255)
gCCGATGCTTGGGCAAAATCATCATTTTAATCACGCAGCCCCCCAAACCATTCCTTACGCTATTGAACGtt < 1:3692163/71‑1 (MQ=255)
gCCGATGCTTGGGCAAAATCATCATTTTAATCACGCAGCCCCCCAAACCATTCCTTACGCTATTGAACGtt < 1:502717/71‑1 (MQ=255)
gCCGATGCTTAGGCAAAATCATGATTTTAATCACGCAGCCCACCAAACCATTCCTTACGCTATTGAACGtt < 1:3972325/71‑1 (MQ=255)
|
CGTGAGCGCGCCGCCACATTACAGTGGTTATTCTGGCAGGTAGGCGGACTGGGGCCGATGCTTGGGCAAAATCATCATTTTAATCACGCAGCCCCCCAAACCATTCCTTACGCTATTGAACGTT > W3110S.gb/2426340‑2426463
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
