Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A1 F28 I1 R2
|
328 |
63.0 |
4687567 |
98.0% |
4593815 |
65.2 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
annotation |
gene |
description |
| RA |
W3110S.gb |
2,430,751 |
G→T |
F204L (TTC→TTA) |
hisQ ← |
histidine/lysine/arginine/ornithine transporter subunit |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | W3110S.gb | 2,430,751 | 0 | G | T | 100.0%
| 147.8
/ NA
| 50 | F204L (TTC→TTA) | hisQ | histidine/lysine/arginine/ornithine transporter subunit |
| Reads supporting (aligned to +/- strand): ref base G (0/0); new base T (19/31); total (19/31) |
TCAGCCCTCTTCACACCCACGGAGTAGCGGCGCTCAAGGAACAGCAGCACACCATTGGAAACAGTGGTGAAAACCAGGTAAATCACGCCACAGACGATGGCGAAATAGAACGGTTCCCAGGTACTTTTGCC > W3110S.gb/2430683‑2430813
|
tCAGCCCTCTTCACACCCACGGAGTAGCGGCGCTCAAGGAACAGCAGCACACCATTGGAAACAGTGGTTaa < 1:3908817/71‑1 (MQ=255)
cccTCTTCACACCCACGGAGTAGCGGCGCTCAAGGAACAGCAGCACACCATTGGAAACAGTGGTTAAAAcc < 1:1642805/71‑1 (MQ=255)
ccTCTTCACACCCACGGAGTAGCGGCGCTCAAGGAACAGCAGCACACCATTGGAAACAGTGGTTAAAAcc < 1:4193979/70‑1 (MQ=255)
ccTCTTCACACCCACGGAGTAGCGGCGCTCAAGGAACAGCAGCACACCATTGGAAACAGTGGTTAAAACCa < 1:4283226/71‑1 (MQ=255)
ttCACACCCACGGAGTAGCGGCGCTCAAGGAACAGCAGCACACCATTGGAAACAGTGGTTAAAACCAgga > 1:4351366/1‑69 (MQ=255)
ttCACACCCACGGAGTAGCGGCGCTCAAGGAACAGCAGCACACCATTGGAAACAGTGGTTAAAACCAgg > 1:773221/1‑69 (MQ=255)
ttCACACCCACGGAGTAGCGGCGCTCAAGGAACAGCAGCACACCATTGGAAACAGTGGTTAAAACCAGGt > 1:4521913/1‑70 (MQ=255)
ttCACACCCACGGAGTAGCGGCGCTCAAGGAACAGCAGCACACCATTGGAAACAGTGGTTAAAACCAGGt > 1:2224928/1‑70 (MQ=255)
cacaCCCACGGAGTAGCGGCGCTCAAGGAACAGCAGCACACCATTGGAAACAGTGGTTaaaa > 1:2386973/1‑62 (MQ=255)
cacaCCCACGGAGTAGCGGCGCTCAAGGAACAGCAGCACACCATTGGAAACAGTGGTTAAAACCAGGTaa < 1:4182675/70‑1 (MQ=255)
acacCCACGGAGTAGCGGCGCTCAAGGAACAGCAGCACACCATTGGAAACAGTGGTTAAAACCAGGTAAAt < 1:4508551/71‑1 (MQ=255)
acacCCACGGAGTAGCGGCGCTCAAGGAACAGCAGCACACCATTGGAAACAGTGGTTAAAACCAGGTAAAt < 1:468438/71‑1 (MQ=255)
acacCCACGGAGTAGCCGCGCTCAAGGAACAGCAGCACACCATTGGAAACAGTGGTTAAAACCAGGTAAAt < 1:650409/71‑1 (MQ=255)
cacCCACGGAGTAGCGGCGCTCAAGGAACAGCAGCACACCATTGGAAACAGTGGTTAAAACCAGGTAAAt > 1:615340/1‑70 (MQ=255)
cccACGGAGTAGCGGCGCTCAAGGAACAGCAGCACACCATTGGAAACAGTGGTTAAAACCAGGTAAATCAc < 1:4027793/71‑1 (MQ=255)
cACGGAGTAGCGGCGCTCAAGGAACAGCAGCACACCATTGGAAACAGTGGTTAAAACCAGGTAAATCACGc > 1:3477494/1‑71 (MQ=255)
cGGAGTAGCGGCGCTCAAGGAACAGCAGCACACCATTGGAAACAGTGGTTAAAACCAGGTAAATCACGCca < 1:1055695/71‑1 (MQ=255)
gAGTAGCGGCGCTCAAGGAACAGCAGCACACCATTGGAAACAGTGGTTAAAACCAGGTAAATCACGCcaca < 1:318080/71‑1 (MQ=255)
tAGCGGCGCTCAAGGAACAGCAGCACACCATTGGAAACAGTGGTTAAAACCAGGTAAATCACGCCACAGAc < 1:2933967/71‑1 (MQ=255)
tAGCGGCGCTCAAGGAACAGCAGCACACCATTGGAAACAGTGGTTAAAACCAGGTAAATCACGCCACAGAc < 1:1643494/71‑1 (MQ=255)
gcggcgCTCAAGGAACAGCAGCACACCATTGGAAACAGTGGTTAAAACCAGGTAAATCACGCCACAGACGa > 1:156103/1‑71 (MQ=255)
cTCAAGGAACAGCAGCACACCATTGGAAACAGTGGTTAAAACCAGGTAAATCACGCCACAGACGATGGCGa < 1:451027/71‑1 (MQ=255)
cTCAAGGAACAGCAGCACACCATTGGAAACAGTGGTTAAAACCAGGTAAATCACGCCACAGACGATGGCGa < 1:4672769/71‑1 (MQ=255)
ggAACAGCAGCACACCATTGGAAACAGTGGTTAAAACCAGGTAAATCACGCCACAGACGATGGCGaaa > 1:2279361/1‑68 (MQ=255)
ggAACAGCAGCACACCATTGGAAACAGTGGTTAAAACCAGGTAAATCACGCCACAGACGATGGCGAAATAg < 1:3717553/71‑1 (MQ=255)
aaCAGCAGCACACCATTGGAAACAGTGGTTAAAACCAGGTaaa > 1:1471100/1‑43 (MQ=255)
gcagcaCACCATTGGAAACAGTGGTTAAAACCAGGTAAATCACGCCACAGACGATGGCGAAATAGAACGGt < 1:2031834/71‑1 (MQ=255)
cagcaCACCATTGGAAACAGTGGTTAAAACCAGGTAAATCACGCCACAGACGATGGCGAAATAGAACGGtt > 1:935181/1‑71 (MQ=255)
cagcaCACCATTGGAAACAGTGGTTAAAACCAGGTAAATCACGCCACAGACGATGGCGAAATAGAACGGtt > 1:1117919/1‑71 (MQ=255)
gcaCACCATTGGAAACAGTGGTTAAAACCAGGTAAATCACGCCACAGAc < 1:2943500/49‑1 (MQ=255)
gcaCACCATTGGAAACAGTGGTTAAAACCAGGTAAATCACGCCACAGAc < 1:2979584/49‑1 (MQ=255)
ccATTGGAAACAGTGGTTAAAACCAGGTAAATCACGCCACAGACGATGGCGAAATAGAACGGtac > 1:1667257/1‑63 (MQ=255)
ccATTGGAAACAGTGGTTAAAACCAGGTAAATCACGCCACAGACGATGGCGAAATAGAACGGTTCCCAgg > 1:2765929/1‑70 (MQ=255)
ccATTGGAAACAGTGGTTAAAAACAGGTAAAACACGCCACAGACGAAGGCg > 1:2843966/1‑51 (MQ=255)
aTTGGAAACAGTGGTTAAAACCAGGTAAATCACGCCACAGACGATGGCGAAATAGAACGGTTCCCAGGTAc < 1:3942230/71‑1 (MQ=255)
aTTGGAAACAGTGGTTAAAACCAGGTAAATCACGCCACAGACGATGGCGAAATAGAACGGTTCCCAGGTAc < 1:1464484/71‑1 (MQ=255)
ttGGAAACAGTGGTTAAAACCAGGTAAATCACGCCACAGAc < 1:346328/41‑1 (MQ=255)
ttGGAAACAGTGGTTAAAACCAGGTAAATCACGCCACAGACGATGGCGAAATAGAACGGTTCCCAGGTACt < 1:3425314/71‑1 (MQ=255)
ttGGAAACAGTGGTTAAAACCAGGTAAATCACGCCACAGACGATGGCGAAATAGAACGGTTCCCAGGTACt < 1:3958133/71‑1 (MQ=255)
ttGGAAACAGTGGTTAAAACCAGGTAAATCACGCCACAGACGATGGCGAAATAGAACGGTTCCCAGGTACt < 1:517063/71‑1 (MQ=255)
ttGGAAACAGTGGTTAAAACCAGGTAAATCACGCCACAGACGATGGCGAAATAGAACGGTTCCCAGGTACt < 1:52487/71‑1 (MQ=255)
ttGGAAACAGTGGTTAAAACCAGGTAAATCACGCCACAGACGATGGCGAAATAGAACGGTTCCCAGGTACt < 1:4079324/71‑1 (MQ=255)
ggAAACAGTGGTTAAAACCAGGTAAATCACGCCACAGACGATGGCGaaa > 1:893052/1‑49 (MQ=255)
ggAAACAGTGGTTAAAACCAGGTAAATCACGCCACAGACGATGGCGAAATAGAACGGTTCCCAGGTACttt > 1:437754/1‑71 (MQ=255)
gAAACAGTGGTTAAAACCAGGTAAATCACGCCACAGACGATGGCGAAATAGAACGGTTCCCAGGTACttt < 1:2836528/70‑1 (MQ=255)
aaaCAGTGGTTAAAACCAGGTAAATCACGCCACAGACGATGGCGAAATAGAACGGTTCCCAGGTACTTTtg < 1:484485/71‑1 (MQ=255)
aaaCAGTGGTTAAAACCAGGTAAATCACGCCACAGACGATGGCGAAATAGAACGGTTCCCAGGTACTTTtg < 1:2222802/71‑1 (MQ=255)
cAGTGGTTAAAACCAGGTAAATCACGCCACAGACGATGGCGAAATAGAACGGTTccc < 1:371583/57‑1 (MQ=255)
cAGTGGTTAAAACCAGGTAAATCACGCCACAGACGATGGCGAAATAGAACGGTTcc > 1:4100943/1‑56 (MQ=255)
cAGTGGTTAAAACCAGGTAAATCACGCCACAGACGATGGCGAAATAGAACGGTTCCCAGGTACTTTtgcc > 1:4066675/1‑70 (MQ=255)
|
TCAGCCCTCTTCACACCCACGGAGTAGCGGCGCTCAAGGAACAGCAGCACACCATTGGAAACAGTGGTGAAAACCAGGTAAATCACGCCACAGACGATGGCGAAATAGAACGGTTCCCAGGTACTTTTGCC > W3110S.gb/2430683‑2430813
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
