Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A1 F21 I0 R1
|
476 |
35.3 |
2812105 |
96.7% |
2719305 |
61.2 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
freq |
annotation |
gene |
description |
| RA |
W3110S.gb |
2,081,459 |
A→G |
10.8% |
F36L (TTT→CTT) |
yeeX ← |
conserved hypothetical protein |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | W3110S.gb | 2,081,459 | 0 | A | G | 10.8%
| 115.2
/ 6.5
| 46 | F36L (TTT→CTT) | yeeX | conserved hypothetical protein |
| Reads supporting (aligned to +/- strand): ref base A (22/19); new base G (2/3); total (24/22) |
| Fisher's exact test for biased strand distribution p-value = 6.59e-01 |
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 |
GGATCTTTTTCTCAACGTCCTGAATTTCACGTTGCAGTTTGTTCTTACGACGGAACAGACGAACAAATTCCAGTACGTCCTGGAATGAAGGCTTGGTAGTTTCCATTTTTATACCCCTGATAATGTGAGAGTCG > W3110S.gb/2081393‑2081526
|
ggATCTTTTTCTCAAGGTCCTGAATTTCACGTTGCAGTTTGTTCTTACGACGGAACAGACGAACAAATTcc < 1:946575/71‑1 (MQ=255)
ggATCTTTTTCTCAACGTCCTGAATTTCACGTTGCAGTTTGTTCTTACGACGGAACAGACGAACAAATTcc < 1:1552593/71‑1 (MQ=255)
cGTCCTGAATTTCACGTTGCAGTTTGTTCTTACGACGGAACAGACGAACAAATTCCAGTACGTCCt > 1:113365/1‑66 (MQ=255)
tCCTGAATTTCACGTTGCAGTTTGTTCTTACGACGGAACAGACGAACAAATTCCAGTACGTCCTGGAATg < 1:1365587/70‑1 (MQ=255)
cTGAATTTCACGTTGCAGTTTGTTCTTACGACGGAACAGACGAACaaa > 1:1218387/1‑48 (MQ=255)
cTGAATTTCACGTTGCAGTTTGTTCTTACGACGGAACAGACGAACAAGTTCCAGTACGTCCTGGAATGAAg > 1:390899/1‑71 (MQ=255)
cTGAATTTCACGTTGCAGTTTGTTCTTACGACGGAACAGACGAACAAGTTCCAGTACGTCCTGGAATGAAg > 1:1371699/1‑71 (MQ=255)
cTGAATTTCACGTTGCAGTTTGTTCTTACGACGGAACAGACGAACAAATTCCAGTACGTCCTGGaa > 1:1101775/1‑66 (MQ=255)
cTGAATTTCACGTTGCAGTTTGTTCTTACGACGGAACAGACGAACAAATTCCAGTACGTCCTGGaa > 1:1125491/1‑66 (MQ=255)
gAATTTCACGTTGCAGTTTGTTCTTACGACGGAACAGACGAACAAATTCCAGTa > 1:455027/1‑54 (MQ=255)
gAATTTCACGTTGCAGTTTGTTCTTACGACGGAACAGACGAACAAATTCCAGTa > 1:614500/1‑54 (MQ=255)
gAATTTCACGTTGCAGTTTGTTCTTACGACGGAACAGACGAACAAATTCCAGTACGTCCTGGAATGAAGGc > 1:2753309/1‑71 (MQ=255)
gAATTTCACGTTGCAGTTTGTTCTTACGACGGAACAGACGAACAAATTCCAGTACGTCCTGGAATGAAGGc > 1:1839158/1‑71 (MQ=255)
tCACGTTGCAGTTTGTTCTTACGACGGAACAGACGAACAAATTCCa > 1:1483735/1‑46 (MQ=255)
tCACGTTGCAGTTTGTTCTTACGACGGAACAGACGAACAAATTCCAGTACGTCCTGGAATGaa > 1:2516577/1‑63 (MQ=255)
tCACGTTGCAGTTTGTTCTTACGACGGAACAGACGAACAAATTCCAGTACGTCCTGGAATGAAGGCTTgg > 1:1362338/1‑70 (MQ=255)
tCACGTTGCAGTTTGTTCTTACGACGGAACAGACGAACAAATTCCAGTACGTCCTGGAATGAAGGCTTgg > 1:2064649/1‑70 (MQ=255)
tCACGTTGCAGTTTGTTCTTACGACGGAACAGACGAACAAATTCCAGTACGTCCTGGAATGAAGGCTTgg < 1:2130154/70‑1 (MQ=255)
gTTGCAGTTTGTTCTTACGACGGAACAGACGAACAAATTCCAGTACGTCCTGGAATGAAGGCTTGGTAGtt < 1:1721911/71‑1 (MQ=255)
gTTGCAGTTTGTTCTTACGACGGAACAGACGAACAAATTCCAGTACGTCCTGGAATGAAGGCTTGGTAGtt < 1:2280990/71‑1 (MQ=255)
cAGTTTGTTCTTACGACGGAACAGACGAACAAATTCCAGTACGTCCTGGAATGAAGGCTTGGTAGt > 1:1060782/1‑66 (MQ=255)
cAGTTTGTTCTTACGACGGAACAGACGAACAAATTCCAGTACGTCCTGGAATGAAGGCTTGGTAGt > 1:961926/1‑66 (MQ=255)
cAGTTTGTTCTTACGACGGAACAGACGAACAAATTCCAGTACGTCCTGGAATGAAGGCTTGGTAGTTTcc < 1:375110/70‑1 (MQ=255)
tttGTTCTTACGACGGAACAGACGAACAAATTCCAGTACGTCCTGGAATGAAGGCTTGGTAGTTTCCAt < 1:2536343/69‑1 (MQ=255)
tGTTCTTTCGACGGAACAGACGAACAAATTCCAGTACGTCCTGGAATGAAGGCTTGGTAGTTTCCAtttt < 1:2597373/70‑1 (MQ=255)
tGTTCTTACGACGGAACAGACGAACAAATTCCAGTACGTCCTGGAATGAAGGc > 1:1961768/1‑53 (MQ=255)
tGTTCTTACGACGGAACAGACGAACAAATTCCAGTACGTCCTGGAATGAAGGc > 1:2534065/1‑53 (MQ=255)
tGTTCTTACGACGGAACAGACGAACAAATTCCAGTACGTCCTGGAATGAAGGCTTGGTAGTTTCCAtttt > 1:2218438/1‑70 (MQ=255)
tGTTCTTACGACGGAACAGACGAACAAATTCCAGTACGTCCTGGAATGAAGGCTTGGTAGTTTCCAtttt > 1:1290238/1‑70 (MQ=255)
tGTTCTTACGACGGAACAGACGAACAAATTCCAGTACGTCCTGGAATGAAGGCTTGGTAGTTTCCAtttt < 1:945015/70‑1 (MQ=255)
tGTTCTTACGACGGAACAGACGAACAAATTCCAGTACGTCCTGGAATGAAGGCTTGGTAGTTTCCAtttt > 1:354538/1‑70 (MQ=255)
tGTTCTTACGACGGAACAGACGAACAAATTCCAGTACGTCCTGGAATGAAGGCTTGGTAGTTTCCAtttt < 1:2143089/70‑1 (MQ=255)
ttCTTACGACGGAACAGACGAACAAGTTCCAGTACGTCCTGGAATGAAGGCTTGGTAGTTTCCATTTTtat < 1:475842/71‑1 (MQ=255)
ttCTTACGACGGAACAGACGAACAAGTTCCAGTACGTCCTGGAATGAAGGCTTGGTAGTTTCCATTTTta < 1:2372379/70‑1 (MQ=255)
ttCTTACGACGGAACAGACGAACAAATTCCAGTACGTCCTGGAATGAAGGCTTGGTAGTTTCCATTTTta < 1:2749419/70‑1 (MQ=255)
ttCTTACGACGGAACAGACGAACAAATTCCAGTACGTCCTGGAATGAAGGCTTGGTAGTTTCCATTTTta < 1:1630043/70‑1 (MQ=255)
ttCTTACGACGGAACAGACGAACAAATTCCAGTACGTCCTGGAATGAAGGCTTGGTAGTTTCCATTTTta < 1:714240/70‑1 (MQ=255)
acgacgGAACAGACGAACAAGTTCCAGTACGTCCTGGAATGAAGGCTTGGTAGTTTCCATTTTTATAcc < 1:164557/69‑1 (MQ=255)
acgacgGAACAGACGAACAAATTCCAGTACGTCCTGGAATGAAGGCTTGGTAGTTTCCAtttt < 1:859970/63‑1 (MQ=255)
cgacgGAACAGACGAACAAATTCCAGTACGTCCTgg < 1:114893/36‑1 (MQ=255)
cgGAACAGACGAACAAATTCCAGTACGTCCTGGAATGAAGGCTTGGTAGTTTCCATTTTTATACCCCTGAt < 1:2762404/71‑1 (MQ=255)
gACGAACAAATTCCAGTACGTCCTGGAATGAAGGCTTg < 1:1349083/38‑1 (MQ=255)
gACGAACAAATTCCAGTACGTCCTGGAATGAAGGCTTGGTAGTTTCCAtttt < 1:743338/52‑1 (MQ=255)
cGAACAAATTCCAGTACGTCCTGGAATGAAGGCTTGGTAGTTTCCATTTTta > 1:1143609/1‑52 (MQ=255)
cGAACAAATTCCAGTACGTCCTGGAATGAAGGCTTGGTAGTTTCCATTTTTATACCCCTGATAAtgtta > 1:2310826/1‑67 (MQ=255)
aaCAAATTCCAGTACGTCCTGGAATGAAGGCTTGGTAGTTTCCATTTTTATACCCCTGATAATGTGagag > 1:576937/1‑70 (MQ=255)
cAAATTCCAGTACGTCCTGGAATGAAGGCTTGGTAGTTTCAATTTTTATACCCCTGATAATGTGAGAGTCg > 1:583477/1‑71 (MQ=255)
aaaTTCCAGTACGTCCTGGAATGAAGGCTTGGTAGTTTCCAtttt > 1:563537/1‑45 (MQ=255)
aaaTTCCAGTACGTCCTGGAATGAAGGCTTGGTAGTTTCCAtttt > 1:560411/1‑45 (MQ=255)
aaaTTCCAGTACGTCCTGGAATGAAGGCTTGGTAGTTTCCAtttt > 1:1488328/1‑45 (MQ=255)
|
GGATCTTTTTCTCAACGTCCTGAATTTCACGTTGCAGTTTGTTCTTACGACGGAACAGACGAACAAATTCCAGTACGTCCTGGAATGAAGGCTTGGTAGTTTCCATTTTTATACCCCTGATAATGTGAGAGTCG > W3110S.gb/2081393‑2081526
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
