Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A1 F8 I0 R1
|
311 |
50.1 |
4085234 |
95.9% |
3917739 |
60.7 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
freq |
annotation |
gene |
description |
| RA |
W3110S.gb |
2,081,459 |
A→G |
25.4% |
F36L (TTT→CTT) |
yeeX ← |
conserved hypothetical protein |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | W3110S.gb | 2,081,459 | 0 | A | G | 25.4%
| 115.3
/ 43.8
| 67 | F36L (TTT→CTT) | yeeX | conserved hypothetical protein |
| Reads supporting (aligned to +/- strand): ref base A (20/30); new base G (11/6); total (31/36) |
| Fisher's exact test for biased strand distribution p-value = 9.64e-02 |
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.90e-01 |
ACGGATCTTTTTCTCAACGTCCTGAATTTCACGTTGCAGTTTGTTCTTACGACGGAACAGACGAACAAATTCCAGTACGTCCTGGAATGAAGGCTTGGTAGTTTCCATTTTTATACCCCTGATAATGTGAGAGTCGGA > W3110S.gb/2081391‑2081528
|
aCGGATCTTTTTCTCAACGTCCTGAATTTCACGTTGCAGTTTGTTCTTACGACGGAACAGACGAACAAAt < 1:2565719/70‑1 (MQ=255)
gATCTTTTTCTCAACGTCCTGAATTTCACGTTGCAGTTTGTTCTTACGACGGAACAGACGAACAAATTCCa < 1:557383/71‑1 (MQ=255)
tCTTTTTCTCAACGTCCTGAATTTCACGTTGCAGTTTGTTCTTACGACGGAACAGACGAACAAATTcc < 1:324786/68‑1 (MQ=255)
cTTTTTCTCAACGTCCTGAATTTCACGTTGCAGTTTGTTCTTACGACGGAACAGACGAACAAATTCCAGTa > 1:1317883/1‑71 (MQ=255)
tttCTCAACGTCCTGAATTTCACGTTGCAGTTTGTTCTTACGACGGAACAGACGAACAAATTCCAg < 1:1067431/66‑1 (MQ=255)
tttCTCAACGTCCTGAATTTCACGTTGCAGTTTGTTCTTACGACGGAACAGACGAACAAATTCCAg < 1:1339754/66‑1 (MQ=255)
tttCTCAACGTCCTGAATTTCACGTTGCAGTTTGTTCTTACGACGGAACAGACGAACAAATTCCAg < 1:3116191/66‑1 (MQ=255)
tttCTCAACGTCCTGAATTTCACGTTGCAGTTTGTTCTTACGACGGAACAGACGAACAAATTCCAGTACg < 1:3425698/70‑1 (MQ=255)
tttCTCAACGTCCTGAATTTCACGTTGCAGTTTGTTCTTACGACGGAACAGACGAACAAATTCCAGTACg < 1:2944437/70‑1 (MQ=255)
aaCGTCCTGAATTTCACGTTGCAGTTTGTTCTTACGACGGAACAGACGAACAAATTCCAGTACGTCCTgg < 1:561689/70‑1 (MQ=255)
aaCGTCCTGAATTTCACGTTGCAGTTTGTTCTTACGACGGAACAGACGAACAAATTCCAGTACGTCCTgg < 1:789766/70‑1 (MQ=255)
gTCCTGAATTTCACGTTGCAGTTTGTTCTTACGACGGAACAGACGAACAAATTCCAGTACGTCCTGGAATg < 1:3854304/71‑1 (MQ=255)
tCCTGAATTTCACGTTGCAGTTTGTTCTTACGACGGAACAGACGAACAAGTTCCAGTACGTCCTgg < 1:3384534/66‑1 (MQ=255)
tCCTGAATTTCACGTTGCAGTTTGTTCTTACGACGGAACAGACGAACAAGTTCCAGTACGTCCTgg < 1:2035175/66‑1 (MQ=255)
tCCTGAATTTCACGTTGCAGTTTGTTCTTACGACGGAACAGACGAACAAGTTCCAGTACGTCCTgg < 1:3092732/66‑1 (MQ=255)
tCCTGAATTTCACGTTGCAGTTTGTTCTTACGACGGAACAGACGAACAAGTTCCAGTACGTCCTgg < 1:139713/66‑1 (MQ=255)
cTGAATTTCACGTTGCAGTTTGTTCTTACGACTGAACAGACGAACAAGTTCCAGTACGTCCTGGaa > 1:2913176/1‑66 (MQ=255)
cTGAATTTCACGTTGCAGTTTGTTCTTACGACGGAACAGACGAACAAGTTCCAGTACGTCCTGGaa > 1:2607045/1‑66 (MQ=255)
cTGAATTTCACGTTGCAGTTTGTTCTTACGACGGAACAGACGAACAAGTTCCAGTACGTCCTGGaa > 1:2873234/1‑66 (MQ=255)
cTGAATTTCACGTTGCAGTTTGTTCTTACGACGGAACAGACGAACAAATTCCAGTACGTCCTGGAATGAAg > 1:343482/1‑71 (MQ=255)
gAATTTCACGTTGCAGTTTGTTCTTACGACGGAACAGACGAACAAATTCCAGTACGTCCTGGAATGAAGGc > 1:1924645/1‑71 (MQ=255)
gAATTTCACGTTGCAGTTTGTTCTTACGACGGAACAGACGAACAAATTCCAGTACGTCCTGGAATGAAGGc > 1:3468426/1‑71 (MQ=255)
gAATTTCACGTTGCAGTTTGTTCTTACGACGGAACAGACGAACAAATTCCAGTACGTCCTGGAATGAAGGc > 1:2637278/1‑71 (MQ=255)
gAATTTCACGTTGCAGTTTGTTCTTACGACGGAACAGACGAACAAATTCCAGTACGTCCTGGAATGAAGGc > 1:1551449/1‑71 (MQ=255)
aaTTTCACGTTGCAGTTTGTTCTTACGACGGAACAGACGAACAAGTTCCAGTACGTCCTGGAATGAAGGc < 1:2620452/70‑1 (MQ=255)
aTTTCACGTTGCAGTTTGTTCTTACGACGGAACAGACGAACAAGTTCCAGTACGTCCTGGAATGaa < 1:1996615/66‑1 (MQ=255)
tttCACGTTGCAGTTTGTTCTTACGACGGAACAGACGAACAAATACCAGTACGTCCTGGAATGAAGGCTTg > 1:1313302/1‑71 (MQ=255)
ttCACGTTGCAGTTTGTTCTTACGACGGAACAGACGAACAAGTTCCAGTACGTCCTGGAATGAAgg > 1:421947/1‑66 (MQ=255)
tCACGTTGCAGTTTGTTCTTACGACGGAACAGACGAACAAATTCCAGTACGTCCTGGAATGAAGGCTTgg > 1:2905752/1‑70 (MQ=255)
cACGTTGCAGTTTGTTCTTACGACGGAACAGACGAACAAGTTCCAGTACGTCCTGGAATGAAGGCTTgg > 1:958428/1‑69 (MQ=255)
cACGTTGCAGTTTGTTCTTACGACGGAACAGACGAACAAATTCCAGTACGTCCTGGAATGAAGGCTTGGt < 1:2673395/70‑1 (MQ=255)
cACGTTGCAGTTTGTTCTTACGACGGAACAGACGAACAAATTCCAGTACGTCCTGGAATGAAGGCTTGGt < 1:797846/70‑1 (MQ=255)
cACGTTGCAGTTTGTTCTTACGACGGAACAGACGAACAAATTCCAGTACGTCCTGGAATGAAGGCTTGGt < 1:1600622/70‑1 (MQ=255)
aCGTTGCAGTTTGTTCTTACGACGGAACAGACGAACAAATTCCAGTACGTCCTGGAATGAAGGCTTGGTa < 1:1291019/70‑1 (MQ=255)
cGTTGCAGTTTGTTCTTACGACGGAACAGACGAACAAATTCCAGTACGTCCTGGAATGAAGGCTTGGTAGt < 1:1167809/71‑1 (MQ=255)
cGTTGCAGTTTGTTCTTACGACGGAACAGACGAACAAATTCCAGTACGTCCTGGAATGAAGGCTTGGTAGt < 1:2564978/71‑1 (MQ=255)
cGTTGCAGTTTGTTCTTACGACGGAACAGACGAACAAATTCCAGTACGTCCTGGAATGAAGGCTTGGTAGt < 1:1692229/71‑1 (MQ=255)
cGTTGCAGTTTGTTCTTACGACGGAACAGACGAACAAATTCCAGTACGTCCTGGAATGAAGGCTTGGTAGt < 1:81412/71‑1 (MQ=255)
cGTTGCAGTTTGTTCTTACGACGGAACAGACGAACAAATTCCAGTACGTCCTGGAATGAAGGCTTGGTAGt < 1:913385/71‑1 (MQ=255)
gTTTGTTCTTACGACGGAACAGACGAACAAATTCCAGTACGTCCTGGAATGAAGGCTTGGTAGTTTCCAtt < 1:1412459/71‑1 (MQ=255)
tGTTCTTACGACGGAACAGACGAACAAGTTCCAGTACGTCCTGGAATGAAGGCTTGGTAGTTTCCAtttt > 1:1072032/1‑70 (MQ=255)
tGTTCTTACGACGGAACAGACGAACAAGTTCCAGTACGTCCTGGAATGAAGGCTTGGTAGTTTCCAtttt > 1:709424/1‑70 (MQ=255)
tGTTCTTACGACGGAACAGACGAACAAGTTCCAGTACGTCCTGGAATGAAGGCTTGGTAGTTTCCAtttt > 1:179184/1‑70 (MQ=255)
tGTTCTTACGACGGAACAGACGAACAAGTTCCAGTACGTCCTGGAATGAAGGCTTGGTAGTTTCCAtttt > 1:1453916/1‑70 (MQ=255)
tGTTCTTACGACGGAACAGACGAACAAATTCCAGTa > 1:1146383/1‑36 (MQ=255)
tGTTCTTACGACGGAACAGACGAACAAATTCCAGTACGTCCTGGAATGAAGGCTTGGTAGTTTCCAtttt > 1:3732136/1‑70 (MQ=255)
tGTTCTTACGACGGAACAGACGAACAAATTCCAGTACGTCCTGGAATGAAGGCTTGGTAGTTTCCAtttt > 1:989196/1‑70 (MQ=255)
tGTTCTTACGACGGAACAGACGAACAAATTCCAGTACGTCCTGGAATGAAGGCTTGGTAGTTTCCAtttt > 1:3129350/1‑70 (MQ=255)
tGTTCTTACGACGGAACAGACGAACAAATTCCAGTACGTCCTGGAATGAAGGCTTGGTAGTTTCCAtttt < 1:2797259/70‑1 (MQ=255)
gTTCTTACGACGGAACAGACGAACAAATTCCAGTACGTCCTGGAATGAAGGCTTGGTAGTTTCCATTTTta > 1:1546811/1‑71 (MQ=255)
gTTCTTACGACGGAACAGACGAACAAATTACAGTACGTCCTGGAATGAAGGCTTgg < 1:1907242/56‑1 (MQ=255)
ttCTTACGACGGAACAGACGAACAAATTCCAGTACGTCCTGGAATGAAGGCTTGGTAGTTTCCAtt < 1:3722542/66‑1 (MQ=255)
ttCTTACGACGGAACAGACGAACAAATTCCAGTACGTCCTGGAATGAAGGCTTGGTAGTTTCCATTTTtat < 1:2682228/71‑1 (MQ=255)
ttCTTACGACGAAACAGACGAACAAATTCCAGTACGTCCTGGAATGAAGGCTTGGTAGTTTCCATTTTta < 1:965175/70‑1 (MQ=255)
cTTACGACGGAACAGACGAACAAATTCCAGTACGTCCTGGAATGAAGGCTTGGTAGTTTCCATTTTTATAc > 1:1627781/1‑71 (MQ=255)
acgGAACAGACGAACAAATTCCAGTACGTCCTGGAATGAAGGCTTGGTAGTTTCCAtttt < 1:1417679/60‑1 (MQ=255)
gAACAGACGAACAAATTCCAGTACGTCCTGGAATGAAGGCTTGGTAGTTTCCAtttt < 1:1848622/57‑1 (MQ=255)
gAACAGACGAACAAATTCCAGTACGTCCTGGAATGAAGGCTTGGTAGTTTCCATTTTTATACCCCTGATaa > 1:1851749/1‑71 (MQ=255)
gAACAGACGAACAAATTCCAGTACGTCCTGGAATGAAGGCTTGGTAGTTTCCATTTTTATACCCCTGATaa > 1:316456/1‑71 (MQ=255)
gAACAGACGAACAAATTCCAGTAAGTCCTGGAATGAAGGCTTGGTAGTTTCCATTTTTATACCCCTGATaa > 1:3938316/1‑71 (MQ=255)
cAGACGAACAAATTCCAGTACGTCCTGGAATGAAGGCTTGGTAGTTTCCAtttt < 1:3577297/54‑1 (MQ=255)
aGACGAACAAGTTCCAGTACGTCCTGGAATGAAGGCTTGGTAGTTTCCATTTTTATACCCCTGATAAtgt > 1:2753974/1‑70 (MQ=255)
gACGAACAAATTCCAGTACGTCCTGGAATGAAGGCTTGGTAGTTTCCATTTTTATACCCCTGATAAtgt > 1:722243/1‑69 (MQ=255)
gAACAAATTGCAGTACGTCCTGGAATGAAGGCTTGGTAGTTTCCATTTTTATACCCCTGATAATGTGagag < 1:845507/71‑1 (MQ=255)
cAAGTTCCAGTACGTCCTGGAATGAAGGCTTGGTAGTTTCCATTTTTATACCCCTGATAATGTGAGAGTc > 1:1319360/1‑70 (MQ=255)
cAAATTCCAGTACGTCCTGGAATGAAGGCTTGGTAGTTTCCATTTTta > 1:1041201/1‑48 (MQ=255)
cAAATTCCAGTACGTCCTGGAATGAAGGCTTGGTAGTTTCCATTTTTATACCCCTGATAAt > 1:27136/1‑61 (MQ=255)
aaaTTCCAGTACGTCCTGGAATGAAGGCTTGGTAGTTTCCATTTTTATACCCCTGATAAtgtg < 1:774934/63‑1 (MQ=255)
aaTTCCAGTACGTCCTGGAATGAAGGCTTGGTAGTTTCCATTTTta < 1:2422114/46‑1 (MQ=255)
aaTTCCAGTACGTCCTGGAATGAAGGCTTGGTAGTTTCCATTTTta < 1:1412823/46‑1 (MQ=255)
aaTTCCAGTACGTCCTGGAATGAAGGCTTGGTAGTTTCCATTTTTATACCCCTGATAATGTGAGAGTCGGa > 1:3109978/1‑71 (MQ=255)
|
ACGGATCTTTTTCTCAACGTCCTGAATTTCACGTTGCAGTTTGTTCTTACGACGGAACAGACGAACAAATTCCAGTACGTCCTGGAATGAAGGCTTGGTAGTTTCCATTTTTATACCCCTGATAATGTGAGAGTCGGA > W3110S.gb/2081391‑2081528
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
