Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A2 F21 I0 R1
|
57 |
0.0 |
661283 |
90.4% |
597799 |
68.0 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
freq |
annotation |
gene |
description |
| RA |
minE |
1,523,658 |
C→T |
100% |
Q153* (CAA→TAA) |
flk → |
predicted flagella assembly protein |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | minE | 1,523,658 | 0 | C | T | 100.0%
| 20.6
/ NA
| 7 | Q153* (CAA→TAA) | flk | predicted flagella assembly protein |
| Reads supporting (aligned to +/- strand): ref base C (0/0); new base T (0/7); total (0/7) |
| Rejected as polymorphism: Frequency below/above cutoff threshold. |
| Rejected as polymorphism: Variant not supported by required number of reads on each strand. |
TTCTCACCCTGTTGCAGGAAGGGAAGATGGTTATTCCGCAACCAC > minE/1523620‑1523664
|
ttCTCACCCTGTTGCAGGAAGGGAAGATGGTTATTCCGTAACcac < 1:205784/45‑1 (MQ=255)
ttCTCACCCTGTTGCAGGAAGGGAAGATGGTTATTCCGTAACcac < 1:234658/45‑1 (MQ=255)
ttCTCACCCTGTTGCAGGAAGGGAAGATGGTTATTCCGTAACcac < 1:392203/45‑1 (MQ=255)
ttCTCACCCTGTTGCAGGAAGGGAAGATGGTTATTCCGTAACcac < 1:4761/45‑1 (MQ=255)
ttCTCACCCTGTTGCAGGAAGGGAAGATGGTTATTCCGTAACcac < 1:556360/45‑1 (MQ=255)
ttCTCACCCTGTTGCAGGAAGGGAAGATGGTTATTCCGTAACcac < 1:557154/45‑1 (MQ=255)
cTGTTGCAGGAAGGGAAGATGGTTATTCCGTAACcac < 1:582640/37‑1 (MQ=255)
|
TTCTCACCCTGTTGCAGGAAGGGAAGATGGTTATTCCGCAACCAC > minE/1523620‑1523664
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
