Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A1 F8 I0 R1
|
311 |
50.1 |
4085234 |
95.9% |
3917739 |
60.7 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
freq |
annotation |
gene |
description |
| RA |
W3110S.gb |
516,807 |
C→T |
36.6% |
E233K (GAA→AAA) |
ybbN ← |
predicted thioredoxin domain‑containing protein |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | W3110S.gb | 516,807 | 0 | C | T | 36.6%
| 37.2
/ 39.4
| 41 | E233K (GAA→AAA) | ybbN | predicted thioredoxin domain‑containing protein |
| Reads supporting (aligned to +/- strand): ref base C (18/8); new base T (11/4); total (29/12) |
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 |
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.94e-01 |
CGCGTCTGACCGTCTGCGGCGGTGAGATCTTTACGCAGATGCCCGAACAGCAACTCCAGCGCCTCTTCATTGCGCCCAACCTGATGCAGTTGCAGCGCCAGTTGCGTCGCCAGTGCGGCATCTTCTGGA > W3110S.gb/516740‑516868
|
cgcgTCTGACCGTCTGCGGCGGTGAGATCTTTACGCAGATGCCCGAACAGCAACTCCAGCGCCTCTTCAt < 1:160597/70‑1 (MQ=255)
cgTCTGACCGTCTGCGGCGGTGAGATCTTTACGCAGATGCCCGAACAGCAACTCCAGCGCCTCTTTATTgc < 1:1314109/71‑1 (MQ=255)
tGACCGTCTGCGGCGGTGAGATCTTTACGCAGATGCCCGAACAGCAACTCCAGCGCCTCTTCATTGCGcc > 1:2484972/1‑70 (MQ=255)
tGACCGTCTGCGGCGGTGAGATCTTTACGCAGATGCCCGAACAGCAACTCCAGCGCCTCTTCATTGCGcc > 1:744631/1‑70 (MQ=255)
tGCGGCGGTGAGATCTTTACGCAGATGCCCGAACAGCAACTCCAGCGCCTCTTCATTGCGCCCAACCTGAt > 1:672406/1‑71 (MQ=255)
tGCGGCGGTGAGATCTTTACGCAGATGCCCGAACAGCAACTCCAGCGCCTCTTCATTGCGCCCAACCTGAt > 1:3610763/1‑71 (MQ=255)
gcggTGAGATCTTTACGCAGATGCCCGAACAGCAACTCCAGCGCCTCTTCATTGCGCCCAACCTGATGCAg > 1:2955242/1‑71 (MQ=255)
gTGAGATCTTTACGCAGATGCCCGAACAGCAACTCCAGCGCCTCTTTATTGCGCCCAACCTGATGCAGTTg > 1:3470256/1‑71 (MQ=255)
gTGAGATCTTTACGCAGATGCCCGAACAGCAACTCCAGCGCCTCTTCATTGCGCCCAACCTGATGCAGTTg > 1:1884469/1‑71 (MQ=255)
gagaTCTTTACGCAGATGCCCGAACAGCAACTCCAGCGCCTCTTCATTGCGCCCAACCTGa > 1:317691/1‑61 (MQ=255)
gagaTCTTTACGCAGATGCCCGAACAGCAACTCCAGCGCCTCTTCATTGCGCCCAACCTGa > 1:312928/1‑61 (MQ=255)
gaTCTTTACGCAGATGCCCGAACAGCAACTCCAGCGCCTCTTCATTGCGCCCAACCTGATGCAGTTGCAg > 1:3901314/1‑70 (MQ=255)
tACGCAGATGCCCGAACAGCAACTCCAGCGCCTCTTTATTGCGCCCAACCTGATGCa > 1:3827722/1‑57 (MQ=255)
tACGCAGATGCCCGAACAGCAACTCCAGCGCCTCTTCATTGCGCCCAACCTGATGCAGTTGCAGCGcc < 1:3146607/68‑1 (MQ=255)
tACGCAGATGCCCGAACAGCAACTCCAGCGCCTCTTCATTGCGCCCAACCTGATACAGTTGCAGCGcc < 1:2056667/68‑1 (MQ=255)
aCGCAGATGCCCGAACAGCAACTCCAGCGCCTCTTTATTGCGCCCAACCTGATGCAGTTGCAGCGCCAGtt > 1:3136956/1‑71 (MQ=255)
aCGCAGATGCCCGAACAGCAACTCCAGCGCCTCTTTATTGCGCCCAACCTGATGCAGTTGCAGCGCCAGtt > 1:2877455/1‑71 (MQ=255)
cAGATGCCCGAACAGCAACTCCAGCGCCTCTTCATTGCGCCCAACCTGATGCAGTTGCAGCGCCAGTTGCg < 1:254622/71‑1 (MQ=255)
cAGATGCCCGAACAGCAACTCCAGCGCCTCTTCATTGCGCCCAACCTGATGCAGTTGAAGCGCCAGTTGCg < 1:1186458/71‑1 (MQ=255)
aTGCCCGAACAGCAACTCCAGCGCCTCTTCATTGCGCCCAACCTGATGCAGt < 1:1742545/52‑1 (MQ=255)
aTGCCCGAACAGCAACTCCAGCGCCTCTTCATTGCGCCCAACCTGATGCAGt < 1:3411565/52‑1 (MQ=255)
tGCCCGAACAGCAACTCCAGCGCCTCTTCATTGCGCCCAACCTGa > 1:1291824/1‑45 (MQ=255)
tGCCCGAACAGCAACTCCAGCGCCTCTTCATTGCGCCCAACCTGATGCAGTTGCa > 1:1331413/1‑55 (MQ=255)
gCCCGAACAGCAACTCCAGCGCCTCTTCATTGCGCCCAACCTGATGCAGTTGCa > 1:1687816/1‑54 (MQ=255)
gCCCGAACAGCAACTCCAGCGCCTCTTCATTGCGCCCAACCTGATGCAGTTGCAGCGCCAGTTGCGTCGc > 1:30336/1‑70 (MQ=255)
cccGAACAGCAACTCCAGCGCCTCTTTATTGCGCCCAACCTGATGCAGTTGCAGCGCCAGTTGCGTCGcc < 1:1391112/70‑1 (MQ=255)
cGAACAGCAACTCCAGCGCCTCTTCATTGCGCCCAACCTGATGCAGTTGCAGCGCCAGTTGCGTCGCCAGt > 1:139949/1‑71 (MQ=255)
cAGCAACTCCAGCGCCTCTTTATTGCGCCCAACCTGATGCAGTTGCAGCGCCAGTTGCGTCGCCAGTGCgg > 1:2068884/1‑71 (MQ=255)
cAGCAACTCCAGCGCCTCTTCATTGCGCCCAACCTGATGCAGTTGCAGCGCCAGTTGCGTCGCCAGTGCgg > 1:382617/1‑71 (MQ=255)
cAGCAACTCCAGCGCCTCTTCATTGCGCCCAACCTGATGCAGTTGCAGCGCCAGTTGCGTCGCCAGTGCg > 1:200957/1‑70 (MQ=255)
aGCAACTCCAGCGCCTCTTCATTGCGCCCAACCTGATGCAGTTGCAGCGcc < 1:184282/51‑1 (MQ=255)
aaCTCCAGCGCCTCTTTATTGCGCCCAACCTGATGCAGTTGCAGCGCCAGTTGCGTCGCCAGTGCGGCAtc > 1:403502/1‑71 (MQ=255)
cTCCAGCGCCTCTTTATTGCGCCCAACCTGATGCAGTTGCAGCGCCAGTTGCGTCGCCAGTGCGGCAtc < 1:2153441/69‑1 (MQ=255)
tCCAGCGCCTCTTTATTGCGCCCAACCTGATGCAGTTGCAGCGCCAGTTGCGTCGCCAGTGCGGCAtctt > 1:3321555/1‑70 (MQ=255)
tCCAGCGCCTCTTTATTGCGCCCAACCTGATGCAGTTGCAGCGCCAGTTGCGTCGCCAGTGCGGCAtctt > 1:3506513/1‑70 (MQ=255)
ccAGCGCCTCTTTATTGCGCCCAACCTGATGCAGTTg > 1:1604249/1‑37 (MQ=255)
ccAGCGCCTCTTTATTGCGCCCAACCTGATGCAGTTg > 1:1941603/1‑37 (MQ=255)
gcCTCTTTATTGCGCCCAACCTGATGCAGTTGCAGCGCCAGTTGCGTCGCCAGTGCGGCATCTTCTGGa > 1:3728830/1‑69 (MQ=255)
tctTTATTGCGCCCAACCTGATGCAGTTGCAGCGCCAGTTGCGTCGCCAGTGCGGCATCTTCTg < 1:1228857/64‑1 (MQ=255)
tCATTGCGCCCAACCTGATGCAGTTGCAGCGCCAGTTGCGTCGCCAg > 1:480422/1‑47 (MQ=255)
tCATTGCGCCCAACCTGATGAAGTTGCAGCGCCAGTTGCGTCGCCAg > 1:3377125/1‑47 (MQ=255)
|
CGCGTCTGACCGTCTGCGGCGGTGAGATCTTTACGCAGATGCCCGAACAGCAACTCCAGCGCCTCTTCATTGCGCCCAACCTGATGCAGTTGCAGCGCCAGTTGCGTCGCCAGTGCGGCATCTTCTGGA > W3110S.gb/516740‑516868
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
