Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A1 F8 I0 R1
|
311 |
50.1 |
4085234 |
95.9% |
3917739 |
60.7 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
freq |
annotation |
gene |
description |
| RA |
W3110S.gb |
1,413,273 |
G→T |
23.1% |
R130R (CGC→CGA) |
ydaO ← |
predicted C32 tRNA thiolase |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | W3110S.gb | 1,413,273 | 0 | G | T | 23.1%
| 145.0
/ 22.1
| 56 | R130R (CGC→CGA) | ydaO | predicted C32 tRNA thiolase |
| Reads supporting (aligned to +/- strand): ref base G (29/14); new base T (10/3); total (39/17) |
| Fisher's exact test for biased strand distribution p-value = 7.33e-01 |
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 |
TATCGTCACGATGGTGACCCAACGCGATCTTCGTCGCCCCCAGTTCCGTTGCGGTACGATAAAGGATACCGCGACGAAGGCGAGAACACAGTGAGCAAGTGGTTTTGCCCTCTGGAATCTTCTCTTTCAC > W3110S.gb/1413203‑1413332
|
tatCGTCACGATGGTGACCCAACGCGATCTTCGTCGCCCCCAGTTCCGTTGCGGTACGATAAAGGATACcg > 1:1729536/1‑71 (MQ=255)
tatCGTCACGATGGTGACCCAACGCGATCTTCGTCGCCCCCAGTTCCGTTGCGGTACGATAAAGGATACcg > 1:1450464/1‑71 (MQ=255)
tCGTCACGATGGTGACCCAACGCGATCTTCGTCGCCCCCAGTTCCGTTGCGGTACGATAAAGGATACcgc < 1:3855314/70‑1 (MQ=255)
tCGTCACGATGGTGACCCAACGCGATCTTCGTCGCCCCCAGTTCCGTTGCGGTACGATAAAGGATACcgc < 1:2587062/70‑1 (MQ=255)
tCGTCACGATGGTGACCCAACGCGATCTTCGTCGCCCCCAGTTCCGTTGCGGTACGATAAAGGATACcgc < 1:1549009/70‑1 (MQ=255)
cGTCACGATGGTGACCCAACGCGATCTTCGTCGCCCCCAGTTCCGTTGCGGTACGATAAAGGATACCTcga > 1:3733934/1‑71 (MQ=255)
cGTCACGATGGTGACCCAACGCGATCTTCGTCGCCCCCAGTTCCGTTGCGGTACGATAAAGGATAACTcga > 1:780204/1‑71 (MQ=255)
gTCACGATGGTGACCCAACGCGATCTTCGTCGCCCCAAGTTCCGTTGCGGTACGATAAAGGATACCGcgac > 1:2726938/1‑71 (MQ=255)
tCACGATGGTGACCCAACGCGATCTTCGTCGCCCCCAGTTCCGTTGCGGTACGATAAAGGATACCGcgacg < 1:1187881/71‑1 (MQ=255)
aCGATGGTGACCCAACGCGATCTTCGTCGCCCCCAGTTCCGTTGCGGTACGATAAAGGATACCGcgacg > 1:632765/1‑69 (MQ=255)
aCGATGGTGACCCAACGCGATCTTCGTCGCCCCCAGTTCCGTTGCGGTACGATAAAGGATACCGcgacg > 1:3542533/1‑69 (MQ=255)
ggTGACCCAACGCGATCTTCGTCGCCCCCAGTTCCGTTGCGGTACGATAAAGGATACCGcga > 1:629754/1‑62 (MQ=255)
ggTGACCCAACGCGATCTTCGTCGCCCCCAGTTCCGTTGCGGTACGATAAAGGATACCGCGACGAAGGc > 1:1875447/1‑69 (MQ=255)
ggTGACCCAACGCGATCTTCGTCGCCCCCAGTTCCGTTGCGGTACGATAAAGGATACCGCGACGAAGGc > 1:3914789/1‑69 (MQ=255)
gTGACCCAACGCGATCTTCGTCGTCCCCAGTTCCGTTGCGGTACGATAAAGGATACCTCGACGAAGGCgag < 1:2666422/71‑1 (MQ=255)
gTGACCCAACGCGATCTTCGTCGCCCCCAGTTCCGTTGCGGTACGATAAAGGATACCGCGACGAAGGc > 1:854874/1‑68 (MQ=255)
tGACCCAACGCGATCTTCGTCGCCCCCAGTTCCGTTGCGGTACGATAAAGGATACCTCGACGAAGGCgag < 1:380640/70‑1 (MQ=255)
cgATCTTCGTCGCCCCCAGTTCCGTTGCGGTACGATAAAGGATACCTCGACGAAGGCGAGAAcaca > 1:2620773/1‑66 (MQ=255)
cgATCTTCGTCGCCCCCAGTTCCGTTGCGGTACGATAAAGGATACCTCGACGAAGGCGAGAAcaca > 1:3893952/1‑66 (MQ=255)
cgATCTTCGTCGCCCCCAGTTCCGTTGCGGTACGATAAAGGATACCTCGACGAAGGCGAGAAcaca > 1:658742/1‑66 (MQ=255)
ttCGTCGCCCCCAGTTCCGTTGCGGTACGATAAAGGATACCGCGACGAAGGCGAGAACACAGTGAGCAAGt > 1:3817437/1‑71 (MQ=255)
cgtcgCCCCCAGTTCCGTTGCGGTACGATAAAGGATACCGCGACGAAGGCGAGAACACAGTGAGc < 1:3108444/65‑1 (MQ=255)
cgtcgCCCCCAGTTCCGTTGCGGTACGATAAAGGATACCGCGACGAAGGCGAGAACACAGTGAGCAAg < 1:3662855/68‑1 (MQ=255)
ccccAGTTCCGTTGCGGTACGATAAAGGATACCGCGACGAAGGCGAGAACACAGTGAGCAAGTGGTTTTg < 1:3821216/70‑1 (MQ=255)
ccccAGTTCCGTTGCGGTACGATAAAGGATACCGCGACGAAGGCGAGAACACAGTGAGCAAGTGGTTTTg < 1:411908/70‑1 (MQ=255)
ccccAGTTCCGTTGCGGTACGATAAAGGATACCGCGACGAAGGCGAGAACACAGTGAGCAAGTGGTTTTg < 1:1706323/70‑1 (MQ=255)
ccccAGTTCCGTTGCGGTACGATAAAGGATACCGCGACGAAGGCGAGAACACAGTGAGCAAGTGGTTTTg < 1:104023/70‑1 (MQ=255)
ccccAGTTCCGTTGCGGTACGATAAAGGATACCGCGACGAAGGCGAGAACACAGTGAGCAAGTGGTTTTg < 1:1263469/70‑1 (MQ=255)
gTTCCGTTGCGGTACGATAAAGGATACCGCGACGAAg > 1:2534936/1‑37 (MQ=255)
gTTCCGTTGCGGTACGATAAAGGATACCGCGACGAAGGCGAGAACACAGTGAGCaa < 1:1457638/56‑1 (MQ=255)
gTTCCGTTGCGGTACGATAAAGGATACCGCGACGAAGGCGAGAACACAGTGAGCAAGTGGTTTTGCCctct > 1:1919666/1‑71 (MQ=255)
gTTCCGTTGCGGTACGATAAAGGATACCGCGACGAAGGCGAGAACACAGTGAGCAAGTGGTTTTGCCctct > 1:2604331/1‑71 (MQ=255)
gTTCCGTTGCGGTACGATAAAGGATACCGCGACGAAGGCGAGAACACAGTGAGCAAGGGGTTTTGCCctct > 1:3690517/1‑71 (MQ=255)
ttCCGTTGCGGTACGATAAAGGATACCTCGACGAAGGCGAGAACACAGTGAGCAAGTGGTTTTGCCCTCTg > 1:3101376/1‑71 (MQ=255)
ttCCGTTGCGGTACGATAAAGGATACCGCGACGAAGGCGAGAACACAGTGAGc < 1:204836/53‑1 (MQ=255)
cGTTGCGGTACGATAAAGGATACCGCGACGAAGGCGAGAACACAGTGAGCAAGTGGTTTTGCCCTCTGGa < 1:1514913/70‑1 (MQ=255)
gCGGTACGATAAAGGATACCTCGACGAAGGCGAGAACACAg > 1:2734672/1‑41 (MQ=255)
gCGGTACGATAAAGGATACCTCGACGAAGGCGAGAACACAGTGAGCAAGTGGTTTTGCCCTCTGGAAtct < 1:1706524/70‑1 (MQ=255)
gCGGTACGATAAAGGATACCGCGACGAAGGCGAGAACACAGTGAGCAAGTGGTTTTGCCCTCTGGAAtctt > 1:1731217/1‑71 (MQ=255)
gCGGTACGATAAAGGATACCGCGACGAAGGCGAGAACACAGTGAGCAAGTGGTTTTGCCCTCTGGAAtctt > 1:1888908/1‑71 (MQ=255)
gCGGTACGATAAAGGATACCGCGACGAAGGCGAGAACACAGTGAGCAAGTGGTTTTGCCCTCTGGAAtctt > 1:3972704/1‑71 (MQ=255)
ggTACGATAAAGGATACCGCGACGAAGGCGAGAACACAGTGAGCAAGTGGTTTTGCCCTCTGGAAtcttct > 1:3234633/1‑71 (MQ=255)
tACGATAAAGGATACCGCGACGAAGGCGAGAACACAGTGAGCAAGTGGTTTTGc > 1:2999739/1‑54 (MQ=255)
tACGATAAAGGATACCGCGACGAAGGCGAGAACACAGTGAGCAAGTGGTTTTGc > 1:2982866/1‑54 (MQ=255)
tACGATAAAGGATACCGCGACGAAGGCGAGAACACAGTGAGCAAGTGGTTTTGc > 1:2508137/1‑54 (MQ=255)
tACGATAAAGGATACCGCGACGAAGGCGAGAACACAGTGAGCAAGTGGTTTTGc > 1:1578277/1‑54 (MQ=255)
aCGATAAAGGATACCGCGACGAAGGCGAGAACACAGTGAGCAAGTGGTTTTGCCCTCTGGAATCTTCTCtt > 1:656976/1‑71 (MQ=255)
aCGATAAAGGATACCGCGACGAAGGCGAGAACACAGTGAGCAAGTGGTTTTGCCCTCTGGAATCTTCTCtt > 1:1917339/1‑71 (MQ=255)
cGATAAAGGATACCTCGACGAAGGCGAGAACACAGTGAGCAAGTGGttt > 1:307108/1‑49 (MQ=255)
cGATAAAGGATACCTCGACGAAGGCGAGAACACAGTGAGCAAGTGGttt > 1:2643647/1‑49 (MQ=255)
cGATAAAGGATACCGGGACGAAGGCGAGAACACAGTGAGCAAGTGGTTTTGCCCTCTGGAATCTTCTCttt > 1:290126/1‑71 (MQ=255)
cGATAAAGGATACCGCGACGAAGGCGAGAACACAGTGAGCAAGTGGTTTTGCCCTCTGGAATCTTCTCttt > 1:1416618/1‑71 (MQ=255)
cGATAAAGGATACCGCGACGAAGGCGAGAACACAGTGAGCAAGTGGTTTTGCCCTCTGGAATCTTCTCttt > 1:3556537/1‑71 (MQ=255)
aTAAAGGATACCGCGACGAAGGCGAGAACACAGTGAGCAAGTGGTTTTGCCCTCTGGAATCTTCTCTTTCa > 1:2229240/1‑71 (MQ=255)
aTAAAGGATACCGCGACGAAGGCGAGAACACAGTGAGCAAGTGGTTTTGCCCTCTGGAATCTTCTCTTTCa > 1:1929514/1‑71 (MQ=255)
aTAAAGGATACCGCGACGAAGGCGAGAACACAGTGAGCAAGTGGTTTTGCCCTCTGGAATCTTCTCTTTCa > 1:1555673/1‑71 (MQ=255)
tAAAGGATACCGCGACGAAGGCGAGAACACAGTGAGCAAGTGGTTTTGCCCTCTGGAATCTTCTCTTTCAc < 1:947189/71‑1 (MQ=255)
tAAAGGATACCGCGACGAAGGCGAGAACACAGTGAGCAAGTGGTTTTGCCCTCTGGAATCTTCTCTTTCAc < 1:3582913/71‑1 (MQ=255)
tAAAGGATACCGAGACGAAGGCGAGAACACAGTGAGCAAGTGGTTTTGCCCTCTGGAATCTTCTCTTTCAc < 1:3883197/71‑1 (MQ=255)
aaaGGATACCTCGACGAAGGCGAGAACACAGTGAGCAAGTGGTTTTGCCCTCTGGAATCTTCTCTATCAc > 1:2499392/1‑70 (MQ=255)
aaaGGATACCGCGACGAAGGCGAGAACACAGTGAGCAAGTGGTTTTGCCCTCTGGAATCTTCTCTTTCAc > 1:3648369/1‑70 (MQ=255)
|
TATCGTCACGATGGTGACCCAACGCGATCTTCGTCGCCCCCAGTTCCGTTGCGGTACGATAAAGGATACCGCGACGAAGGCGAGAACACAGTGAGCAAGTGGTTTTGCCCTCTGGAATCTTCTCTTTCAC > W3110S.gb/1413203‑1413332
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
