Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A1 F17 I0 R2
|
276 |
45.3 |
3729697 |
93.0% |
3468618 |
60.6 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
freq |
annotation |
gene |
description |
| RA |
W3110S.gb |
3,404,820 |
G→A |
52.1% |
intergenic (+507/‑471) |
yhdH → / → accB |
predicted oxidoreductase, Zn‑dependent and NAD(P)‑binding/acetyl CoA carboxylase, BCCP subunit |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | W3110S.gb | 3,404,820 | 0 | G | A | 52.1%
| ‑2.6
/ 69.8
| 48 | intergenic (+507/‑471) | yhdH/accB | predicted oxidoreductase, Zn‑dependent and NAD(P)‑binding/acetyl CoA carboxylase, BCCP subunit |
| Reads supporting (aligned to +/- strand): ref base G (10/13); new base A (16/9); total (26/22) |
| Fisher's exact test for biased strand distribution p-value = 2.46e-01 |
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.69e-01 |
TCGTCTATCTTGTCGCGATCCTGGCATCCCTACATTATTTGTGGTCTGTGAAGATTATCTCATTGCAGCCCCTCATCTTCGCAGGGCTGGCTTTTCAGCTTTTCACCTTACGTTATAAGAAGTTCCGTCGAT > W3110S.gb/3404753‑3404884
|
tcgtcTATCTTGTCGCGATCCTGGCATCCCTACATTATTTGTGGTCTGTGAAGATTATCTCATTGCAGc > 1:3310065/1‑69 (MQ=255)
gtcTATCTTGTCGCGATCCTGGCATCCCTACATTATTTGTGGTCTGTGAAGATTATCTCATTGCAGcc < 1:1867950/68‑1 (MQ=255)
cTATCTTGTCGCGATCCTGGCATCCCTACATTATTTGTGGTCTGTGAAGATTATCTCATTGCAAcccc < 1:1468062/68‑1 (MQ=255)
cTATCTTGTCGCGATCCTGGCATCCCTACATTATTTGTGGTCTGTGAAGATTATCTCATTGCAAcccc < 1:430083/68‑1 (MQ=255)
cTATCTTGTCGCGATCCTGGCATCCCTACATTATTTGTGGTCTGTGAAGATTATCTCATT‑CAGCCCCTc < 1:1895160/69‑1 (MQ=255)
tATCTTGTCGCGATCCTGGCATCCCTACATTATTTGTGGTCTGTGAAGATTATCTCATTGCAGCCCCTc > 1:390320/1‑69 (MQ=255)
tATCTTGTCGCGATCCTGGCATCCCTACATTATTTGTGGTCTGTGAAGATTATCTCATTGCAACCCCTc > 1:999002/1‑69 (MQ=255)
tATCTTGTCGCGATCCTGGCATCCCTACATTATTTGTGGTCTGTGAAGATTATCTCATTGCAACCCCTc > 1:62284/1‑69 (MQ=255)
ttGTCGCGATCCTGGCATCCCTACATTATTTGTGGTCTGTGAAGATTATCTCATTGCAGCCCCTCAt < 1:3623465/67‑1 (MQ=255)
ttGTCGCGATCCTGGCATCCCTACATTATTTGTGGTCTGTGAAGATTATCTCATTGCAGCCCCTCATc < 1:1682636/68‑1 (MQ=255)
ttGTCGCGATCCTGGCATCCCTACATTATTTGTGGTCTGTGAAGATTATCTCATTGCAGCCCCTCATc < 1:1409821/68‑1 (MQ=255)
cgcgATCCTGGCATCCCTACATTATTTGTGGTCTGTGAAGATTATCTCATTGCAGCCCCTCAt < 1:784706/63‑1 (MQ=255)
cgATCCTGGCATCCCTACATTATTTGTGGTCTGTGAAGATTATCTCATTGCAACCCCTCATCTTCGCAg > 1:1932209/1‑69 (MQ=255)
ccTACATTATTTGTGGTCTGTGAAGATTATCTCATTGCAGCCCCTCATCTTCGCAGGGCTGgcttttca > 1:1943147/1‑69 (MQ=255)
ccTACATTATTTGTGGTCTGTGAAGATTATCTCATTGCAGCCCCTCATCTTCGCAGGGCTGgcttttca > 1:945221/1‑69 (MQ=255)
ccTACATTATTTGTGGTCTGTGAAGATTATCTCATTGCAGCCCCTCATCTTCGCAGGGCTGgcttttca > 1:1226851/1‑69 (MQ=255)
ccTACATTATTTGTGGTCTGTGAAGATTATCTCATTGCAGCCCCTCATCTTCGCAGGGCTGgcttttca > 1:3237031/1‑69 (MQ=255)
tACATTATTTGTGGTCTGTGAAGATTATCTCATTGCAACCCCTCATCTTCGCAGGGCTGgcttttcag < 1:1448215/68‑1 (MQ=255)
tACATTATTTGTGCTCTGTGAAGATTATCTCATTGCAACCCCTCATCTTCGCAGGGCTGgcttttcag < 1:2850484/68‑1 (MQ=255)
cATTATTTGTGGTCTGTGAAGATTATCTCATTGCAGCCCCt > 1:1199924/1‑41 (MQ=255)
cATTATTTGTGGTCTGTGAAGATTATCTCATTGCAGCCCCTCATCTTCg > 1:1994684/1‑49 (MQ=255)
cATTATTTGTGGTCTGTGAAGATTATCTCATTGCAGCCCCTCATCTTCGCAGGGCTGgcttttcagc > 1:3006575/1‑67 (MQ=255)
cATTATTTGTGGTCTGTGAAGATTATCTCATTGCAACCCCt > 1:673702/1‑41 (MQ=255)
ttattTGTGGTCTGTGAAGATTATCTCATTGCAGCCCCTc < 1:2715337/40‑1 (MQ=255)
ttattTGTGGTCTGTGAAGATTATCTCATTGCAGCCCCTc < 1:3019476/40‑1 (MQ=255)
ttattTGTGGTCTGTGAAGATTATCTCATTGCAGCCCCTc < 1:2676569/40‑1 (MQ=255)
ttattTGTGGTCTGTGAAGATTATCTCATTGCAGCCCCTCATCTTCGCAGGGCTGgcttttcagctttt < 1:1260903/69‑1 (MQ=255)
ttattTGTGGTCTGTGAAGATTATCTCATTGCAACCCCTCATCTTCGCAGGGCTGgcttttcagctttt < 1:3662964/69‑1 (MQ=255)
attTGTGGTCTGTGAAGATTATCTCATTGCAGccc > 1:3647733/1‑35 (MQ=255)
attTGTGGTCTGTGAAGATTATCTCATTGCAACCCCTCATCTTCg > 1:1590977/1‑45 (MQ=255)
attTGTGGTCTGTGAAGATTATCTCATTGCAACCCCTCATCTTCGCAGGGCTGgcttttcagcttttcc > 1:3338292/1‑68 (MQ=255)
attTGTGGTCTGTGAAGATTATCTCATTGCAACCCCTCATCTTCGCAGGGCTGgcttttcagcttttca > 1:2718937/1‑69 (MQ=255)
attTGTGGTCTGTGAAGATTATCTCATTGCAACCCCTCATCTTCGCAGGGCTGgcttttcagcttttca > 1:604638/1‑69 (MQ=255)
attTGTGGTCTGTGAAGATTATCTCATTGCAACCCCTCATCTTCGCAGGGCTGgcttttcag > 1:3470083/1‑62 (MQ=255)
attTGTGGTCTGTGAAGATTATCTCATTGCAACCCCTCATCTTCGCAGGGCTGgcatttcag > 1:1074554/1‑62 (MQ=255)
gtgGTCTGTGAAGATTATCTCATTGCAACCCCTCATCTTCGCAGGGCTGGCTTTTCAGCTTTTCACCtt > 1:3150165/1‑69 (MQ=255)
gtgGTCTGTGAAGATTATCTCATTGCAACCCCTCATCTTCGCAGGGCTGGCTTTTCAGCTTTTCACCt > 1:2830312/1‑68 (MQ=255)
ggTCTGTGAAGATTATCTCATTGCAACCCCTCATCTTCGCAGGGCTGGCTTTTCAGCTTTTCACCTTa < 1:3107741/68‑1 (MQ=255)
ggTCTGTGAAGATTATCTCATTGCAACCCCTCATCTTCGCAGGGCTGGCTTTTCAGCTTTTCACCTTa < 1:1326863/68‑1 (MQ=255)
gAAGATTATCTCATTGCAGCCCCTCATCTTCGCAGGGCTGGCTTTTCAGCTTTTCACCtt < 1:3185848/60‑1 (MQ=255)
gAAGATTATCTCATTGCAGCCCCTCATCTTCGCAGGGCTGGCTTTTCAGCTTTTCACCtt < 1:2501587/60‑1 (MQ=255)
gATTATCTCATTGCAACCCCTCATCTTCGCAGGGCTGgcttttcagcttt > 1:476510/1‑50 (MQ=255)
gATTATCTCATTGCAACCCCTCATCTTCGCAGGGCTGGCTTTTCAGCTTTTCACCTTACGTTATaag > 1:1740903/1‑67 (MQ=255)
tATCTCATTGCAACCCCTCATCTTCGCAGGGCTGGCTTTTCAGCTTTTCACCTTACGTTATAAGAAGtt > 1:3108095/1‑69 (MQ=255)
ctcATTGCAACCCCTCATCTTCGCAGGGCTGGCTTTTCAGCTTTTCACCTTACGTTATAAGAAGTTCCg > 1:2733419/1‑69 (MQ=255)
cATTGCAACCCCTCATCTTCGCAGGGCTGgcttttca < 1:2334596/37‑1 (MQ=255)
tGCAGCCCCTCATCTTCGCAGGGCTGGCTTTTCAGCTTTTCACCTTACGTTATAAGAAGTTCCGTCgat < 1:2268812/69‑1 (MQ=255)
tGCAACCCCTCATCTTCGCAGGGCTGGCTTTTCAGCTTTTCACCTTACGTTATAAGAAGTTCCGTCgat < 1:389784/69‑1 (MQ=255)
|
TCGTCTATCTTGTCGCGATCCTGGCATCCCTACATTATTTGTGGTCTGTGAAGATTATCTCATTGCAGCCCCTCATCTTCGCAGGGCTGGCTTTTCAGCTTTTCACCTTACGTTATAAGAAGTTCCGTCGAT > W3110S.gb/3404753‑3404884
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
