Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A1 F3 I0 R1
|
560 |
56.4 |
4650532 |
94.9% |
4413354 |
59.3 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
freq |
annotation |
gene |
description |
| RA |
W3110S.gb |
3,404,829 |
C→G |
18.4% |
intergenic (+516/‑462) |
yhdH → / → accB |
predicted oxidoreductase, Zn‑dependent and NAD(P)‑binding/acetyl CoA carboxylase, BCCP subunit |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | W3110S.gb | 3,404,829 | 0 | C | G | 18.4%
| 125.6
/ 22.1
| 60 | intergenic (+516/‑462) | yhdH/accB | predicted oxidoreductase, Zn‑dependent and NAD(P)‑binding/acetyl CoA carboxylase, BCCP subunit |
| Reads supporting (aligned to +/- strand): ref base C (25/23); new base G (4/7); total (29/31) |
| Fisher's exact test for biased strand distribution p-value = 5.06e-01 |
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.79e-01 |
TTGTCGCGATCCTGGCATCCCTACATTATTTGTGGTCTGTGAAGATTATCTCATTGCAGCCCCTCATCTTCGCAGGGCTGGCTTTTCAGCTTTTCACCTTACGTTATAAGAAGTTCCGTCGATGATGGCGCTAATTT > W3110S.gb/3404762‑3404898
|
ttGTCGCGATCCTGGCATCCCTACATTATTTGTGGTCTGTGAAGATTATCTCATTGCAGCCCCTCATGTTc > 1:2500991/1‑71 (MQ=255)
gTCGCGATCCTGGCATCCCTACATTATTTGTGGTCTGTGAAGATTATCTCATTGCAGCCCCTCATCTTCg < 1:3993300/70‑1 (MQ=255)
tCGCGATCCTTGCATCCCTACATTATTTGTGGTCTGTGAAGATTATCTCATTGCAGCCCCTCATCTTCGCa < 1:2532224/71‑1 (MQ=255)
tCGCGATCCTGGCATCCCTACATTATTTGTGGTCTGTGAAGATTATCTCATTGCAGCCCCTCATCt < 1:3134286/66‑1 (MQ=255)
gcgATCCTGGCATCCCTACATTATTTGTGGTCTGTGAAGATTATCTCATTGCAGCCCCTCATCTTCGCAg < 1:1225222/70‑1 (MQ=255)
ccTGGCATCCCTACATTATTTGTGGTCTGTGAAGATTATCTCATTGCAGCCCCTCATGTTCGCAGggctg > 1:1755356/1‑70 (MQ=255)
cTGGCATCCCTACATTATTTGTGGTCTGTGAAGATTATCTCATTGCAGCCCCTCATCTTCGCAGggctggc > 1:232711/1‑71 (MQ=255)
cTGGCATCCCTACATTATTTGTGGTCTGTGAAGATTATCTCATTGCAGCCCCTCATCTTCGCAGggctggc > 1:2284170/1‑71 (MQ=255)
ggCATCCCTACATTATTTGTGGTCTGTGAAGATTATCTCATTGCAGCCCCTCATCTTCGCAGGGCTGgctt > 1:1057619/1‑71 (MQ=255)
ggCATCCCTACATTATTTGTGGTCTGTGAAGATTATCTCATTGCAGCCCCTCATCTTCGCAGGGCTGgctt > 1:2660788/1‑71 (MQ=255)
gCATCCCTACATTATTTGTGGTCTGTGAAGATTATCTCATTGCAGCCCCTCATCTTCGCAGGGCTGgctt < 1:456655/70‑1 (MQ=255)
tCCCTACATTATTTGTGGTCTGTGAAGATTATCTCATTGCAGCCCCTCATCTTCg < 1:2832553/55‑1 (MQ=255)
ccTACATTATTTGTGGTCTGTGAAGATTATCTCATTGCAGCCCCTCATCTTCGCAGggctgg > 1:1527993/1‑62 (MQ=255)
ccTACATTATTTGTGGTCTGTGAAGATTATCTCATTGCAGCCCCTCATCTTCGCAGggct > 1:1410422/1‑60 (MQ=255)
ccTACATTATTTGTGGTCTGTGAAGATTATCTCATTGCAGCCCCTCATCTTCGCAGGGCTGgcttttcagc > 1:1055616/1‑71 (MQ=255)
ccTACATTATTTGTGGTCTGTGAAGATTATCTCATTGCAGCCCCTCATCTTCGCAGGGCTGgcttttcagc > 1:3903040/1‑71 (MQ=255)
tACATTATTTGTGGTCTGTGAAGATTATCTCATTGCAGCCCCTCATCTTc < 1:1413741/50‑1 (MQ=255)
cATTATTTGTGGTCTGTGAAGATTATCTCATTGCAGCCCCTCATCTTCGCa > 1:1746200/1‑51 (MQ=255)
cATTATTTGTGGTCTGTGAAGATTATCTCATTGCAGCCCCTCATCTTCGCAGGGCTGgcttttcagctttt > 1:3605287/1‑71 (MQ=255)
cATTATTTGTGGTCTGTGAAGATTATCTCATTGCAGCCCCTCATCTTCGCAGGGCTGgcttttcagctttt > 1:2532355/1‑71 (MQ=255)
attattTGTGGTCTGTGAAGATTATCTCATTGCAGCCCCTCATCTTCGCAGGGCTGgcttttcagcttttc < 1:314889/71‑1 (MQ=255)
ttattTGTGGTCTGTGAAGATTATCTCATTGCAGCCCCTCATGTTCGCAGTGCTGgcttttcagcttttca < 1:621613/71‑1 (MQ=255)
ttattTGTGGTCTGTGAAGATTATCTCATTGCAGCCCCTCATGTTCGCAGGGCTGgcttttcagcttttca < 1:270712/71‑1 (MQ=255)
ttattTGTGGTCTGTGAAGATTATCTCATTGCAGCCCCTCATCTTCGCAGGGCTGgcttttc < 1:4359250/62‑1 (MQ=255)
tattTGTGGTCTGTGAAGATTATCTCATTGCAGCCCCTCATGTTCGCAGGGCTGGCTTTTCAGCTTTTCAc > 1:3324647/1‑71 (MQ=255)
tattTGTGGTCTGTGAAGATTATCTCATTGCAGCCCCTCATGTTCGCAGGGCTGGCTTTTCAGCTTTTCAc > 1:856261/1‑71 (MQ=255)
tattTGTGGTCTGTGAAGATTATCTCATTGCAGCCCCTCATCTTCGCAGGGCTGGCTTTTCAGCTTTTCAc > 1:2161732/1‑71 (MQ=255)
attTGTGGTCTGTGAAGATTATCTCATTGCAGCCCCTCATCTTc > 1:99272/1‑44 (MQ=255)
attTGTGGTCTGTGAAGATTATCTCATTGCAGCCCCTCATCTTCGCAGGGCTGgcttttcag > 1:1587896/1‑62 (MQ=255)
tttGTGGTCTGTGAAGATTATCTCATTGCAGCCCCTCATGTTCGCAGGGCTGGCTTTTCAGCTTTTCACCt < 1:430309/71‑1 (MQ=255)
tttGTGGTCTGTGAAGATTATCTCATTGCAGCCCCTCATGTTCGCAGGGCTGGCTTTTCAGCTTTTCACCt < 1:3765975/71‑1 (MQ=255)
tgtgGTCTGTGAAGATTATCTCATTGCAGCCCCTCATGTTCGCAGGGCTGGCTTTTCAGCTTTTCACCtt < 1:1726970/70‑1 (MQ=255)
ggTCTGTGAAGATTATCTCATTGCAGCCCCTCATCTTCGc < 1:242233/40‑1 (MQ=255)
ggTCTGTGAAGATTATCTCATTGCAGCCCCTCATCTTCGc < 1:3372139/40‑1 (MQ=255)
tCTGTGAAGATTATCTCATTGCAGCCCCTCATCTTCGCAGGGCTGGCTTTTCAGCTTTTCACCTTACGTta < 1:1531834/71‑1 (MQ=255)
tgtgAAGATTATCTCATTGCAGCCCCTCATCTTCGCAGGGCTGGCTTTTCAGCTTTTCACCTTACGTta > 1:3595052/1‑69 (MQ=255)
gAAGATTATCTCATTGCAGCCCCTCATCTTCGCAGGGCTGGCTTTTCAGCTTTTGACCTTACGTTATaag > 1:432656/1‑70 (MQ=255)
gAAGATTATCTCATTGCAGCCCCTCATCTTCGCAGGGCTGGCTTTTCAGCTTTTCACCTTACGTTATaag > 1:2199307/1‑70 (MQ=255)
aaGATTATCTCATTGCAGCCCCTCATCTTCGCAGGGCTGGCTTTTCAGCTTTTCACCTTACGTTATaaga < 1:2936107/70‑1 (MQ=255)
aaGATTATCTCATTGCAGCCCCTCATCTTCGCAGGGCTGGCTTTTCAGCTTTTCACCTTACGTTATaaga < 1:1722490/70‑1 (MQ=255)
aGATTATCTCATTGCAGCCCCTCATCTTCGCAGGGCTGGCTTTTCAGCTTTTCACCTTACGTTATaagaag < 1:1306317/71‑1 (MQ=255)
gATTATCTCATTGCAGCCCCTCATCTTCGCAGGGCTGGCTTTTCAGCTTTTCACCTTACGTTATAAGAAGt < 1:524240/71‑1 (MQ=255)
aTTATCTCATTGCAGCCCCTCATGTTCGCAGGGCTGGCTTTTCAGCTTTTCACCTTACGTtat < 1:2252226/63‑1 (MQ=255)
aTTATCTCATTGCAGCCCCTCATATTCGCAGGGCTGGCTTTTCAGCTTTTCAACTTACGTTATAAGAAGtt < 1:2381939/71‑1 (MQ=38)
tATCTCATTGCAGCCCCTCATCTTCGCAGggctgg > 1:1046318/1‑35 (MQ=255)
tATCTCATTGCAGCCCCTCATCTTCGCAGGGCTGGCTTTTCAGCTTTTCACCTTACGTTATAAGAAGTTcc > 1:3581945/1‑71 (MQ=255)
aTTGCAGCCCCTCATCTTCGCAGGGCTGGCTTTTCAGCTTTTCACCTTACGTTATAAGAAGTTCCGTc < 1:2725095/68‑1 (MQ=255)
aTTGCAGCCCCTCATCTTCGCAGGGCTGGCTTTTCAGCTTTTCACCTTACGTTATAAGAAGTTCCGTc < 1:1909635/68‑1 (MQ=255)
aTTGCAGCCCCTCATCTTCGCAGGGCTGGCTTTTCAGCTTTTCACCTTACGTTATAAGAAGTTCCGTc < 1:4597275/68‑1 (MQ=255)
aTTGCAGCCCCTCATCTTCGCAGGGCTGGCTTTTCAGCTTTTCACCTTACGTTATAAGAAGTTCCGTc < 1:1668229/68‑1 (MQ=255)
aTTGCAGCCCCTCATCTTCGCAGGGCTGGCTTTTCAGCTTTTCACCTTACGTTATAAGAAGTTCCGTc < 1:3576632/68‑1 (MQ=255)
ccccTCATCTTCGCAGGGCTGGCTTTTCAGCTTTTCACCTTACGTTATaaga > 1:4203393/1‑52 (MQ=255)
cTCATGTTCGCAGGGCTGGCTTTTCAGCTTTTCACCTTACg < 1:2904677/41‑1 (MQ=255)
tCATCTTCGCAGGGCTGGCTTTTCAGCTTTTCACCTTACGTTATAAGAAGTTCCGTCgatga > 1:4391525/1‑62 (MQ=255)
tCATCTTCGCAGGGCTGGCTTTTCAGCTTTTCACCTTACGTTATAAGAAGTTCCGTCgatg > 1:4019015/1‑61 (MQ=255)
cATCTTCGCAGGGCTGGCTTTTCAGCTTTTCACCTTACGTTATaag < 1:2769524/46‑1 (MQ=255)
cATCTTCGCAGGGCTGGCTTTTCAGCTTTTCACCTTACGTTATAAGAAGTTCCGTCga > 1:4545412/1‑58 (MQ=255)
cATCTTCGCAGGGCTGGCTTTTCAGCTTTTCACCTTACGTTATAAGAAGTTCCGTCGATGATGGCGCTAAt > 1:3455215/1‑71 (MQ=255)
tCTTCGCAGGGCTGGCTTTTCAGCTTTTCACCTTACGTTATaaga < 1:3462048/45‑1 (MQ=255)
tCTTCGCAGGGCTGGCTTTTCAGCTTTTCACCTTACGTTATAAGAAGTTCCGTCGATGATGGCGCTAAttt > 1:3242236/1‑71 (MQ=255)
|
TTGTCGCGATCCTGGCATCCCTACATTATTTGTGGTCTGTGAAGATTATCTCATTGCAGCCCCTCATCTTCGCAGGGCTGGCTTTTCAGCTTTTCACCTTACGTTATAAGAAGTTCCGTCGATGATGGCGCTAATTT > W3110S.gb/3404762‑3404898
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
