Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A1 F8 I0 R1
|
311 |
50.1 |
4085234 |
95.9% |
3917739 |
60.7 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
freq |
annotation |
gene |
description |
| RA |
W3110S.gb |
3,404,829 |
C→G |
31.3% |
intergenic (+516/‑462) |
yhdH → / → accB |
predicted oxidoreductase, Zn‑dependent and NAD(P)‑binding/acetyl CoA carboxylase, BCCP subunit |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | W3110S.gb | 3,404,829 | 0 | C | G | 31.3%
| 81.6
/ 50.2
| 67 | intergenic (+516/‑462) | yhdH/accB | predicted oxidoreductase, Zn‑dependent and NAD(P)‑binding/acetyl CoA carboxylase, BCCP subunit |
| Reads supporting (aligned to +/- strand): ref base C (26/20); new base G (10/11); total (36/31) |
| Fisher's exact test for biased strand distribution p-value = 6.00e-01 |
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.98e-01 |
CTTGTCGCGATCCTGGCATCCCTACATTATTTGTGGTCTGTGAAGATTATCTCATTGCAGCCCCTCATCTTCGCAGGGCTGGCTTTTCAGCTTTTCACCTTACGTTATAAGAAGTTCCGTCGATGATGGCGCTAA > W3110S.gb/3404761‑3404895
|
cTTGTCGCGATCCTGGCATCCCTACATTATTTGTGGTCTGTGAAGATTATCTCATTGCAGCCCCTCATCtt > 1:3284454/1‑71 (MQ=255)
gTCGCGATCCTGGCATCCCTACATTATTTGTGGTCTGTGAAGATTATCTCATTGCAGCCCCTCATCTTCg > 1:779181/1‑70 (MQ=255)
tCGCGATCCTGGCATCCCTACATTATTTGTGGTCTGTGAAGATTATCTCATTGCAGCCCCTCATGTTCGc < 1:2519527/70‑1 (MQ=255)
gggATCCTGGCATCCCTACATTTTTTGTGGTCTGTGAAGATTATCTCATTGCAGCCCCTCATCTTCGCAgg < 1:284931/69‑1 (MQ=255)
cgATCCTGGCATCCCTACATTATTTGTGGTCTGTGAAGATTATCTCATTGCAGCCCCTCATCTTCGCAgg < 1:541849/70‑1 (MQ=255)
aTCCTGGCATCCCTACATTATTTGTGGTCTGTGAAGATTATCTCATTGCAGCCCCTCATCTTCGCAGggct < 1:960671/71‑1 (MQ=255)
aTCCTGGCATCCCTACATTATTTGTGGTCTGTGAAGATTATCTCATTGCAGCCCCTCATCTTCGCAGggct < 1:407808/71‑1 (MQ=255)
cTGGCATCCCTACATTATTTGTGGTCTGTGAAGATTATCTCATTGCAGCCCCTCATGTTCGCAGggctgg < 1:3950550/70‑1 (MQ=255)
cTGGCATCCCTACATTATTTGTGGTCTGTGAAGATTATCTCATTGCAGCCCCTCATGTTCGCAGggctgg < 1:3033417/70‑1 (MQ=255)
ggCATCCCTACATTATTTGTGGTCTGTGAAGATTATCTCATTGCAGCCCCTCATCTTCGCAGGGCTGgctt > 1:110712/1‑71 (MQ=255)
ggCATCCCTACATTATTTGTGGTCTGTGAAGATTATCTCATTGCAGCCCCTCATCTTCGCAGGGCTGgctt > 1:2342106/1‑71 (MQ=255)
gCATCCCTACATTATTTGTGGTCTGTGAAGATTATCTCATTGCAGCCCCTCATGTTCGCAGGGCTGgcttt < 1:2612832/71‑1 (MQ=255)
ccTACATTATTTGTGGTCTGTGAAGATTATCTCATTGCAGCCCCTCATGTTCGCAGGGCTGgcttttcagc < 1:2450972/71‑1 (MQ=255)
ccTACATTATTTGTGGTCTGTGAAGATTATCTCATTGCAGCCCCTCATGTTCGCAGGGCTGgcttttcagc > 1:2947892/1‑71 (MQ=255)
ccTACATTATTTGTGGTCTGTGAAGATTATCTCATTGCAGCCCCTCATCTTCGCAGGGCTGgcttttcagc > 1:2761726/1‑71 (MQ=255)
cATTATTTGTGGTCTGTGAAGATTATCTCATTGCAGCCCCTCATGTTCGCAGGGCTGgcttttcagctttt > 1:1134486/1‑71 (MQ=255)
cATTATTTGTGGTCTGTGAAGATTATCTCATTGCAGCCCCTCATGTTCGCAGGGCTGgcttttcagctttt > 1:1763465/1‑71 (MQ=255)
cATTATTTGTGGTCTGTGAAGATTATCTCATTGCAGCCCCTCATGTTCGCAGGGCTGgcttttcagctt < 1:2929794/69‑1 (MQ=255)
cATTATTTGTGGTCTGTGAAGATTATCTCATTGCAGCCCCTCATCTTCGCAg > 1:630452/1‑52 (MQ=255)
cATTATTTGTGGTCTGTGAAGATTATCTCATTGCAGCCCCTCATCTTCGCAGGGCTGgcttttcagctttt > 1:1025237/1‑71 (MQ=255)
cATTATTTGTGGTCTGTGAAGATTATCTCATTGCAGCCCCTCATCTTCGCAGGGCTGgcttttcagctttt > 1:2814651/1‑71 (MQ=255)
cATTATTTGTGGTCTGTGAAGATTATCTCATTGCAGCCCCTCATCTTCGCAGGGCTGgcttttcagctttt > 1:533322/1‑71 (MQ=255)
attattTGTGGTCTGTGAAGAATATCTCATTGCAGCCCCTCATCTTCGCAGGGCTGgcttttcagctttt > 1:553761/1‑70 (MQ=255)
ttattTGTGGTCTGTGAAGATTATCTCATTGCAGCCCCTCATCTTCGCAGGGCTGgcttttcagcttttca > 1:2700442/1‑71 (MQ=255)
tattTGTGGTCTGTGAAGATTATCTCATTGCAGCCCCTCATGTTCGCAGggctg > 1:1449926/1‑54 (MQ=255)
tattTGTGGTCTGTGAAGATTATCTCATTGCAGCCCCTCATGTTCGCAGGGCTGgcttttcagcttttca > 1:406749/1‑70 (MQ=255)
attTGTGGTCTGTGAAGATTATCTCATTGCAGCCCCTCATGTTCGCAGGGCTGGCTTTTCAGCTTTTCAc < 1:2461004/70‑1 (MQ=255)
tgtgGTCTGTGAAGATTATCTCATTGCAGCCCCTCATCTTCGCAGGGCTGGCTTTTCAGCTTTTCACCtt > 1:326326/1‑70 (MQ=255)
gTCTGTGAAGATTATCTCATTGCAGCCCCTCATCTTCGCAGGGCTGGCTTTTCAGCTTTTCACCTTACGt < 1:3941537/70‑1 (MQ=255)
gTCTGTGAAGATTATCTCATTGCAGCCCCTCATCTTCGCAGGGCTGGCTTTTCAGCTTTTCACCTTACGt < 1:679963/70‑1 (MQ=255)
tCTGTGAAGATTATCTCATTGCAGCCCCTCATCTTCGCAGGGCTGgcttttcagcttttca < 1:3128216/61‑1 (MQ=255)
tCTGTGAAGATTATCTCATTGCAGCCCCTCATCTTCGCAGGGCTGGCTTTTCAGCTTTTCACCTTACGt < 1:3724326/69‑1 (MQ=255)
tgtgAAGATTATCTCATTGCAGCCCCTCATCTTCGCAGGGCTGgcttttcagct > 1:2441564/1‑54 (MQ=255)
tgtgAAGATTATCTCATTGCAGCCCCTCATCTTCGCAGGGCTGGCTTTTCAGCTTTTCACCTTACGt > 1:432170/1‑67 (MQ=255)
tgtgAAGATTATCTCATTGCAGCCCCTCATCTTCGCAGGGCTGGCTTTTCAGCTTTTCACCTTACGTtat > 1:2938370/1‑70 (MQ=255)
tgAAGATTATCTCATTGCAGCCCCTCATGTTCGCAGGGCTGGCTTTTCAGCTTTTCACCTTACGTtat > 1:1186297/1‑68 (MQ=255)
tgAAGATTATCTCATTGCAGCCCCTCATCTTCGCAGGGCTGGCTTTTCAGCTTTTCACCTTACGTtata < 1:1660773/69‑1 (MQ=255)
tgAAGATTATCTCATTGCAGCCCCTCATCTTCGCAGGGCTGGCTTTTCAGCTTTTCACCTTACGTtata < 1:1533662/69‑1 (MQ=255)
tgAAGATTATCTCATTGCAGCCCCTCATCTTCGCAGGGCTGGCTTTTCAGCTTTTCACCTTACGTtat < 1:702399/68‑1 (MQ=255)
aGATTATCTCATTGCAGCCCCTCATCTTCGCAGggctggct < 1:1328749/41‑1 (MQ=255)
ttATCTCATTGCAGCCCCTCATGTTCGCAGGGCTGgcttttcagct < 1:117550/46‑1 (MQ=255)
ttATCTCATTGCAGCCCCTCATCTTCGCAGGGCAGGCTTTTCAGCTTTTCACCTTACGTTATAAGAAGtt > 1:2351032/1‑70 (MQ=255)
ttATCTCATTGCAGCCCCTCATCTTCGCAGGGCAGGCTTTTCAGCTTTTCACCTTACGTTATAAGAAGt > 1:1273267/1‑69 (MQ=255)
aTCTCATTGCAGCCCCTCATGTTCGCAGGGCTGgcttttcagcttt > 1:1634419/1‑46 (MQ=255)
aTCTCATTGCAGCCCCTCATGTTCGCAGGGCTGgcttttcagcttt > 1:3894893/1‑46 (MQ=255)
aTTGCAGCCCCTCATCTTCGCAGGGCTGGCTTTTCAGCTTTTCACCTTACGTTATAAGAAGTTCCGTc < 1:3310243/68‑1 (MQ=255)
aTTGCAGCCCCTCATCTTCGCAGGGCTGGCTTTTCAGCTTTTCACCTTACGTTATAAGAAGTTCCGTc < 1:2370121/68‑1 (MQ=255)
aTTGCAGCCCCTCATCTTCGCAGGGCTGGCTTTTCAGCTTTTCACCTTACGTTATAAGAAGTTCCGTc < 1:3047169/68‑1 (MQ=255)
aTTGCAGCCCCTCATCTTCGCAGGGCTGGCTTTTCAGCTTTTCACCTTACGTTATAAGAAGTTCCGTCgat < 1:3255104/71‑1 (MQ=255)
tGCAGCCCCTCATGTTCGCAGGGCTGGCTTTTCAGCTTTTCACCTTACGTTATAAGAAGTTCCGTCgatga > 1:2836976/1‑71 (MQ=255)
gCAGCCCCTCATGTTCGCAGGGCTGGCTTTTCAGCTTTTCACCTTACGTTATAAGAAGTTCCGTCgatgat > 1:3595236/1‑71 (MQ=255)
gCAGCCCCTCATCTTCGAAGGGCTGGCTTTTCAGCTTTTCACATTACGTTATAAGAAGTTCCGTCgatga > 1:3135594/1‑70 (MQ=255)
cAGCCCCTCATCTTCGCAGGGCTGGCTTTTCAGCTTTTCACCTTACGTTATAAGAAGTTCCGTCgatgat < 1:3052343/70‑1 (MQ=255)
cAGCCCCTCATCTTCGCAGGGCTGGCTTTTCAGCTTTTCACCTTACGTTATAAGAAGTTCCGTCgatgat < 1:1367316/70‑1 (MQ=255)
cccTCATGTTCGCAGGGCTGGCTTTTCAGCTTTTCACCTTACGTTATAAGAAGTTCCGTCgat < 1:3852411/63‑1 (MQ=255)
cccTCATGTTCGCAGGGCTGGCTTTTCAGCTTTTCACCTTACGTTATAAGAAGTTCCGTCgat < 1:2527212/63‑1 (MQ=255)
cccTCATGTTCGCAGGGCTGGCTTTTCAGCTTTTCACCTTACGTTATAAGAAGTTCCGTCgat < 1:1441325/63‑1 (MQ=255)
tCATCTTCGCAGGGCTGGCTTTTCAGCTTTTCACCTTACGTTATAAGAAGTTCCGTCgatgat < 1:3962385/63‑1 (MQ=255)
tCATCTTCGCAGGGCTGGCTTTTCAGCTTTTCACCTTACGTTATAAGAAGTTCCGTCgatgat < 1:3229720/63‑1 (MQ=255)
tCATCTTCGCAGGGCTGGCTTTTCAGCTTTTCACCTTACGTTATAAGAAGTTCCGTCGATTATGGCGCTaa > 1:458343/1‑71 (MQ=255)
cATCTTCGCAGGGCTGGCTTTTCAGCTTTTCACCTTACGTTATAAGAAGTTCCGTCGATGATgg > 1:157287/1‑64 (MQ=255)
cATCTTCGCAGGGCTGGCTTTTCAGCTTTTCACCTTACGTTATAAGAAGTTCCGTCGATGATgg > 1:741996/1‑64 (MQ=255)
cATCTTCGCAGGGCTGGCTTTTCAGCTTTTCACATTACGTTATAAGAAGTTCCGTCGATGATgg > 1:1907927/1‑64 (MQ=255)
aTCTTCGCAGGGCTGGCTTTTCAGCTTTTCACCTTACGTTATaagaag > 1:2348530/1‑48 (MQ=255)
aTCTTCGCAGGGCTGGCTTTTCAGCTTTTCACCTTACGTTATaagaag > 1:3739735/1‑48 (MQ=255)
tCTTCGCAGGGCTGGCTTTTCAGCTTTTCACCTTACGTTATaagaag > 1:2337159/1‑47 (MQ=255)
tCTTCGCAGGGCTGGCTTTTCAGCTTTTCACCTTACGTTATaagaag > 1:194821/1‑47 (MQ=255)
|
CTTGTCGCGATCCTGGCATCCCTACATTATTTGTGGTCTGTGAAGATTATCTCATTGCAGCCCCTCATCTTCGCAGGGCTGGCTTTTCAGCTTTTCACCTTACGTTATAAGAAGTTCCGTCGATGATGGCGCTAA > W3110S.gb/3404761‑3404895
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
