Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A10 F210 I0 R1 188 159.1 7866933 93.3% 7339848 99.8

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 3,459,293:1 (G)5→6 100% coding (955/1482 nt) gspE → general secretory pathway component, cryptic

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,459,2881.G91.0% 493.4 / 42.6 156coding (950/1482 nt)gspEgeneral secretory pathway component, cryptic
Reads supporting (aligned to +/- strand):  ref base . (7/7);  new base G (63/79);  total (70/86)
Fisher's exact test for biased strand distribution p-value = 7.81e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 166 total aligned reads displayed.

GGAAGGCATTGGGCAAACGCAGGTGAATACCCGTGTGGATATGTCTTTCGCTCGCGGCCTGCGCGCCATACTTCGCCAGGACCCGGATGTCGTCATG‑GT‑GG‑GGG‑AAATTCGTGATACAGAAACCGCGCAGATTGCGGTTCAGGCCTCGCTCACCGGCCATCTGGTACTCTCAACACTCCACACTAACAGTGCA  >  NC_000913/3459190‑3459382
                                                                                                    |                                                                                                
ggAAGGCATTGGGCAAACGCAGGTGAATACCCGTGTGGATATGTCTTTCGCTCGCGGCCTGCGCGCCATACTTCGCCAGGACCCGGATGTCGTCAtg‑gt‑g                                                                                                 <  1:2426247/100‑1 (MQ=255)
  aaGGCATTGGGCAAACGCAGGTGAATACCCGAGTGGATATGTATTTCGCTCGCGGCCTGCGCGCCATACTTCGCCAGAACCCGGATGTCGTCATG‑GT‑gg‑g                                                                                              >  2:264087/1‑100 (MQ=255)
   aGGCATTGGGCAAACGCAGGTGAATACCCGTGTGGATATGTCTTTCGCTCGCGGCCTGCGCGCCATACTTCGCCAGGACCCGGATGTCGTCATG‑GT‑gg‑gg                                                                                             >  2:2435394/1‑100 (MQ=255)
   aGGCATTGGGCAAACGCAGGTGAATACCCGTGTGGATATGTCTTTCGCTCGCGGCCTGCGCGCCATACTTCGCCAGGACCCGGATGTCGTCATG‑GT‑gg‑gg                                                                                             <  2:1176903/100‑1 (MQ=255)
     gCATTGGGCAAACGCAGGTGAATACCCGTGTGGATATGTCTTTCGCTCGCGGCCTGCGCGCCATACTTCGCCAGGACCCGGATGTCGTCATG‑GT‑gg‑gggg                                                                                           >  1:1564041/1‑99 (MQ=255)
     gCATTGGGCAAACGCAGGTGAATACCCGTGTGGATATGTCTTTCGCTCGCGGCCTGCGCGCCATACTTCGCCACGACCCGGATGTCGTCATG‑GT‑gg‑gggg                                                                                           <  1:2385744/100‑2 (MQ=255)
      cATTGGGCAAACGCAGGTGAATACCCGTGTGGATATGTCTTTCGCTCGCGGCCTGCGCGCCATACTTAGCCAGGACCCGGATGTCGTCATG‑GT‑gg‑gggga                                                                                          >  1:807962/1‑98 (MQ=255)
          gggCAAACGCAGGTGAATACCCGTGTGGATATGTATTTCGCTCGCGGCCTGCGCGCCATACTTCGCCAGGACCCGGATGTCGTCATG‑GT‑GG‑GGG‑gaaa                                                                                       >  2:1528657/1‑97 (MQ=255)
           ggCAAACGCAGGTGAATACCCGTGTGGATATGTCTTTCGCTCGCGGCCTGCGCGCCATACTTCACCAAGACCCGGATGTCGTCATG‑GTGGG‑GGG‑AAATTc                                                                                     >  1:745498/1‑100 (MQ=255)
             cAAACGCAGGTGAATACCCGTGTGGATATGTCTTTCGCTCGCGGCCTGCGCGCCATACTTCGCCAGGACCCGGATGTCGTCATG‑GTGGG‑GGG‑AAATTCGt                                                                                   <  1:875662/100‑1 (MQ=255)
             cAAACGCAGGTGAATACCCGTGTGGATATGTCTTTCGCTCGCGGCCTGCGCGCCATACTTCGCCAGGACCCGGATGTCGTCATG‑GTGGG‑GGG‑AAATTCGt                                                                                   <  1:2018410/100‑1 (MQ=255)
              aaaCGCAGGTGAATACCCGTGTGGATATGTCTTTCGCTCGCGGCCTGCGCGCCATACTTCGCCAGGACCCGGATGTCGTCATG‑GTGGG‑GGG‑AAATTCGTg                                                                                  <  1:3034146/100‑1 (MQ=255)
              aaaCGCAGGTGAATACCCGTGTGGATATGTCTTTCGCTCGCGGCCTGCGCGCCATACTTCGCCAGGACCCGGATGTCGTCATG‑GTGGG‑GGG‑AAATTCGTg                                                                                  <  1:3079002/100‑1 (MQ=255)
              aaaCGCAGGTGAATACCCGTGTGGATATGTCATTCGCTCGCGGCCTGCGCGCCATACTTCGCCAGGACCCGGATGTCGTCATG‑GTGGG‑GGG‑AAATTCGTg                                                                                  <  2:3157669/100‑1 (MQ=255)
              aaaCGCAGGTGAATACCCGTGTGGATATGCCTTTCGCTCGCGGCCTGCGCGCCATACTTCGCCAGGACCCGGATGTCGTCATG‑GTGGG‑GGG‑AAATTCGTg                                                                                  <  2:2318129/100‑1 (MQ=255)
               aaCGCAGGTGAATTCCCGTGTGGATATGTCTTTCGCTCGCGGCCTGCGCGCCATACTTCGCCAGGACCCGGATGTCGTCATG‑GTGGG‑GGG‑AAATTCGTGa                                                                                 <  2:3759424/100‑1 (MQ=255)
               aaCGCAGGTGAATACCCGTGTGGATATGTCTTTCGCTCGCGGCCTGCGCGCCATACTTCGCCAGGACCCGGATGTCGTCATG‑GTGGG‑GGG‑AAATTCGTGa                                                                                 >  2:1738296/1‑100 (MQ=255)
               aaCGCAGGTGAATACCCGTGTGGATATGTCTTTCGCTCGCGGCCTGCGCGCCATACTTCGCCAGGACCCGGATGTCGTCATG‑GTGGG‑GGG‑AAATTCGTGa                                                                                 <  2:38364/100‑1 (MQ=255)
                aCGCAGGTGAATACCCGTGTGGATATGTCTTTCGCTCGCGGCCTGCGCGCCATACTTCGCCAGGACCCGGATGTCGTCATG‑GTGGG‑GGG‑AAATTCGTGAt                                                                                <  1:1980929/100‑1 (MQ=255)
                aCGCAGGTGAATACCCGTGTGGATATGTCTTTCGCTCGCGGCCTGCGCGCCATACTTCGCCAGGACCCGGATGTCGTCATG‑GTGGG‑GGG‑AAATTCGTGAt                                                                                <  1:533009/100‑1 (MQ=255)
                  gCAGGTGAATACCCGTGTGGATATGTCTTTCGCTCGCGGCCTGCGCGCCATACTTCGCCAGGACCCGGATGTCGTCATG‑GTGGG‑GGG‑AAATTCGTGATAc                                                                              <  2:1977761/100‑1 (MQ=255)
                     ggTGAATACCCGTGTGGATATGTCTTTCGCTCGCGGCCTGCGCGCCATACTTCGCCAGGACCCGGATGTCGTCATG‑GTGGG‑GGG‑AAATTCGTGATACAGa                                                                           >  2:721575/1‑100 (MQ=255)
                     ggTGAATACCCGTGTGGATATGTCTTTCGCTCGCGGCCTGCGCGCCATACTTCGCCAGGACCCGGATGTCGTCATG‑GTGGG‑GGG‑AAATTCGTGATACAGa                                                                           <  1:3049571/100‑1 (MQ=255)
                       tGAATACCCGTGTGGATATGTCTTTCGCTCGCGGCCTGCGCGCCATACTTCGCCAGGACCCGGATGTCGTCATG‑GTGGG‑GGG‑AAATTCGTGATACAGaaa                                                                         >  2:2493647/1‑100 (MQ=255)
                        gAATACCCGTGTGGATATGTCTTTCGCTCGCGGCCTGCGCGCCATTCTTCGCCAGGACCCGGATGTCGTCATG‑GTGGG‑GGG‑AAATTCGTGATACAGAAAc                                                                        <  1:2965775/100‑1 (MQ=255)
                         aaTACCCGTGTGGATATGTTTTTCGCTCGCGGCCTGCGCGCCATACTTCGCCAGTACCCGGATGTCGTCATG‑GTGGG‑GGG‑AAATTCGTGATAc                                                                              <  2:2972509/93‑1 (MQ=255)
                           tACCCGTGTGGATATGTCTTTCGCTCGCGGCCTGCGCGCCATACTTCGCCAGTACCCTGATGTCGTCATG‑GTGGG‑GGG‑AAAATCGTGATACAGAAACcgc                                                                     >  1:1942717/1‑100 (MQ=255)
                           tACCCGTGTGGATATGTCTTTCGCTCGCGGCCTGCGCGCCATACTTCGCCAGGACCCGGATGTCGTCATG‑GTGGG‑GGG‑AAATTCGTGATACAGAAACcgc                                                                     >  1:3887744/1‑100 (MQ=255)
                            aCCCGTGTGGATATGTCTTTCGGTCGCGGCCTGCGTGCCATACTTCGCCAGGACCCGGATGTCGTCATG‑GTGGG‑GGG‑AAATTCGTGATACAGAAACcgcg                                                                    <  1:3656896/100‑1 (MQ=255)
                             cccGTGTGGATATGTCTTTCGCTCGCGGCCTGCGCGCCATACTTCGCCAGGACCCGGATGTCGTCATG‑GTGGG‑GGG‑AAATTCGTGATACAGAAACcgcg                                                                    <  1:2727438/99‑1 (MQ=255)
                             cccGTGTGGATATGTCTTTCGCTCGCGGCCTGCGCGCCATACTTCGCCAGGACCCGGATGTCGTCATG‑GA‑GGAGGG‑CAATTCGTGATACAGAAAc                                                                        >  2:3712279/1‑95 (MQ=255)
                              ccGTGTGGATATGTCTTTCGCTCGCGGCCTGCGCGCCATACTTCGCCAGGACCCGGATGTCGTCATG‑GTGGG‑GGG‑AAATTCGTGATACAGAAACCGCGCa                                                                  >  2:2658918/1‑100 (MQ=255)
                              ccGTGTGGAGATGTCTTTCGCTCGCGGCCTGCGCGCCATACTTCGCCAGGACCCGGATGTCGTCATG‑GTGGG‑GGG‑AAATTCGTGATACAGAAACCgcgc                                                                   <  2:3841699/99‑1 (MQ=255)
                                   tgGATATGTCTTTCGCTCGCGGCCTGCGCGCCATACTTCGCCAGGACCCGGATGTAGTCATG‑GTGGG‑GGG‑AAATTCGTGATACAGAAACCGCGCAGATTg                                                             >  1:1233821/1‑100 (MQ=255)
                                    ggATATGTCTTTCGCTCGCGGCGTGCGCGCCATAGTTCGCCAGGACCCGGATGTCGTCATG‑GTGGG‑GGG‑AAATTCGTGATACAGAAACCGCGCAGATTg                                                             >  2:3898609/1‑99 (MQ=255)
                                    ggATATGTCTTTCGCTCGCGGCCTGCGCGCCATACTTCGCCAGGACCCGGATGTCGTCATG‑GTGGG‑GGG‑AAATTCGTGATACAGAAACCGCGCAGATTGc                                                            <  2:591501/100‑1 (MQ=255)
                                    ggATATGTCTTTCGCTCGCGGCCTGCGCGCCATACTTCGCCAGGACCCGGATGTCGTCATG‑GTGGG‑GGG‑AAATTCGTGATACAGAAACCGCGCAGATTGc                                                            >  1:3684740/1‑100 (MQ=255)
                                     gATATGTCTTTCGCTCGCGGCCTGCGCGCCATACTTCGCCAGGACCCGGATGTCGTCATG‑GT‑GGAAGG‑AAATTCGAGATACAGAAACCGCGCAGATTGCg                                                           >  1:470094/1‑100 (MQ=255)
                                     gATATGTCTTTCACTCGCGGCCTGCGCGCCATACTTCGCCAGGACCCGGATGTCATCATG‑GTGGG‑GGG‑AAATTCGTGATACAGAAACCGCGCAGATTGCg                                                           >  1:63820/1‑100 (MQ=255)
                                      atatGTCTTTCGCTCGCGGCCTGCGCGCCATACTTCGCCAGGACCCGGATGTCGTCATG‑GTGGG‑GGG‑AAATTCGTGATACAGAAACCGCGCAGATTGCgg                                                          <  2:2822286/100‑1 (MQ=255)
                                      atatGTCTTTCGCTCGCGGCCTGCGCGCCATACTTCGCCAGGACCCGGATGTCGTCATG‑GTGGG‑GGG‑AAATTCGTGATACAGAAACCGCGCAGATTGCgg                                                          <  2:2820630/100‑1 (MQ=255)
                                       tatGTCTTTCGCTCGGGGCCTGCGCGCCATACTTCGCCAGGACCCGGATGTCGTCATG‑GTGGG‑GGG‑AAATTCGTGATACAGAAACCGCGCAGATTGCgg                                                          <  2:3437345/99‑1 (MQ=255)
                                       tatGTCTTTCGCTCGCGGCCTGCGCGCCATACTTCGCCAGGACCCGGATGTCGTCATG‑GTGGG‑GGG‑AAATTCGTGATACAGAAGCCGCGCAGATTGCgg                                                          <  2:1163057/99‑1 (MQ=255)
                                       tatGTCTTTCGCTCGCGGCCTGCGCGCCATACTTCGCCAGGACCCGGATGTCGTCATG‑GTGGG‑GGG‑AAATTCGTGATACAGAAACCGCGCAGATTGCgg                                                          <  1:3561865/99‑1 (MQ=255)
                                       tatGTCTTTCGCTCGCGGCCTGCGCGCCATACTTCGCCAGGACCCGGATGTCGTCATG‑GTGGG‑GGG‑AAATTCGTGATACAGAAACCGCGCAGATTGCGGt                                                         <  2:3197010/100‑1 (MQ=255)
                                            cTTTCGCTCGCGGCCTGCGCGCCATACTTCGCCAGGACCCGGATGTCGTCATG‑GTGGG‑GGG‑AAATTCGTGATACAGAAACCGCGCAGATTGCGGTTCAgg                                                    >  2:1680715/1‑100 (MQ=255)
                                            cTTTCGCTCGCGGCCTGCGCGCCATACTTCGCCAGGACCCGGATGTCGTCATG‑GTGGG‑GGG‑AAATTCGTGATACAGAAACCGCGCAGATTGCGGTTCAgg                                                    >  1:3220493/1‑100 (MQ=255)
                                            cTTTCGCTCGCGGCCTGCGCGCCATACTTCGCCAGGACCCGGATGTCGTCATG‑GTGGG‑GGG‑AAATTCGTGATACAGAAACCGCGCAGATTGCGGTTCAgg                                                    >  1:2203764/1‑100 (MQ=255)
                                            cTTTCGCTCGCGGCCTGCGCGCCATACTTCGCCAGGACCCGGATGTCGTCATG‑GTGGG‑GGG‑AAATTCGTGATACAGAAACCGCGCAGATTGCGGTTCAgg                                                    >  1:808900/1‑100 (MQ=255)
                                            cTTTCGCTCGCGGCCTGCGCGCCATACTTCGCCAGGACCCGGATGTCGTCATG‑GTGGG‑GGG‑AAATTCGTGATACAGAAACCGCGCAGATTGCGGTTCAgg                                                    <  1:2135048/100‑1 (MQ=255)
                                             tttCGCTCGCGGCCTGCGCGCCATACTTCGCCAGGACCCGGATGTCGTCATG‑GTGGG‑GGG‑AAATTCGTGATACAGAAACCGCGCAGATTGCGGTTCAGGc                                                   >  1:397045/1‑100 (MQ=255)
                                             tttCGCTCGCGGCCTGCGCGCCATACTTCGCCAGGACCCGGATGTCGTCATG‑GTGGG‑GGG‑AAATTCGTGATACAGAAACCGCGCAGATTGCGGTTCAGGc                                                   <  1:3341724/100‑1 (MQ=255)
                                                 gctcgcGGCCTGCGCGCCATACTTCGCCAGGACCCGGATGTCGTCATG‑GTGGG‑GGG‑AAATTCGTGATACAGAAACCGCGCAGATTGCGGTTCAGGcn                                                  <  2:107784/97‑2 (MQ=255)
                                                  ctcgcGGCCTGCGCGCCATACTTCGCCAGGACCCGGATGTCGTCATG‑GTGGG‑GGG‑AAATTCGTGATACAGAAACCGCGCAGATTGCGGTTCAGGCCTCGc                                              <  2:1915769/100‑1 (MQ=255)
                                                  ctcgcGGCCTGCGCGCCATACTTCGCCAGGACCCGGATGTCGTCATG‑GTGGG‑GGG‑AAATTCGTGATACAGAAACCGCGCAGATTGCGGTTCAGGCCTCGc                                              >  1:12159/1‑100 (MQ=255)
                                                  ctcgcGGCCTGCGCGCCATACTTCGCCAGGACCCGGATGTCGTCATG‑GTGGG‑GGG‑AAATTCGTGATACAGAAACCGCGCAGATTGCGGTTCAGGCCTCGc                                              >  1:2324356/1‑100 (MQ=255)
                                                  ctcgcGGCCTGCGCGCCATACTTCGCCAGGACCCGGATGTCGTCATG‑GTGGG‑GGG‑AAATTCGTGATACAGAAACCGCGCAGATTGCGGTTCAGGCCTCGc                                              >  1:660038/1‑100 (MQ=255)
                                                  ctcgcGGCCTGCGCGCCATACTTCGCCAGGACCCGGATGTCGTCATG‑GTGGG‑GGG‑AAATTCGTGATACAGAAACCGCGCAGATTGCGGTTCAGGCCTCGc                                              <  1:3270303/100‑1 (MQ=255)
                                                    cgtggtcTGCGCGCTATACTTCGCCAGGAAGCGGATGTCGACATG‑GTGGG‑GGG‑AAAGTCGTGGACCATAAAACGCGACGATTGCGGTTCAg                                                     >  2:2690425/7‑91 (MQ=255)
                                                     gcgGCCTGCGCGCCATACTTCGCCAGGACCCGGATGTCGTCATG‑GTGGG‑GGG‑AAATTCGTGATACAGAAACCGCGCAGATTGCGGTTCAGGCCTCGCTCa                                           <  1:1390634/100‑1 (MQ=255)
                                                       ggCCTGCGCGCCATACTTCGCCAGGACCCGGATGTCGTCATG‑GTGGG‑GGG‑AAATTCGTGATACAGAAACCGCGCAGATTGCGGTTCAGGCCTCGCTCAcc                                         >  1:3623041/1‑100 (MQ=255)
                                                       ggCATGCGCGCCATACTTCGCCAGGACCCGGATTTCGTCATG‑GTGGG‑GGG‑AAATTCGTGATACAGAAACCGCGCAGATTGCGGTTCAGGCCTCGCTCAcc                                         <  2:3802217/100‑1 (MQ=255)
                                                         ccTGTGCGCCATACTTCGCCATGACCCGGATGTCGTCATG‑GTGGG‑GGG‑AAATTCGTGATACAGAAACCGCGCAGATTGCGGTTCAGGCCTCGCTCACCgg                                       <  1:2681252/100‑1 (MQ=255)
                                                          cTGCGCGCCATACTTCGCCAGGACCCGGATGTCGTCATG‑GTGGG‑GGG‑AAATTCGTGATACAGAAACCGCGCAGATTGCGGTTCAGGCCTCGCTCACCGGc                                      >  2:2247117/1‑100 (MQ=255)
                                                          cTGCGCGCCATACTTCGCCAGGACCCGGATGTCGTCATG‑GTGGG‑GGG‑AAATTCGTGATACAGAAACCGCGCAGATTGCGGTTCAGGCCTCGCTCACCGGc                                      <  2:3333623/100‑1 (MQ=255)
                                                             cgcgcCATACTTCGCCAGGACCCGGATGTCGTCATG‑GTGGG‑GGG‑AAATTCGTGATACAGAAACCGCGCAGATTGCGGTTCAGGCCTCGCTCACCGGCCAt                                   <  1:2957187/100‑1 (MQ=255)
                                                              gcgcCATACTTCGCCAGGACCCGGATGTCGTCATG‑GTGGG‑GGG‑AAATTCGTGATACAGAAACCGCGCAGATTGCGGTTCAGGCCTCGCTCACCGGCCATc                                  >  2:1312532/1‑100 (MQ=255)
                                                                gcCATACTTCGCCAGAACCCGGATGTCGTCATG‑GTGGG‑GGG‑AAATTCGTGATACAGAAACCGCGCAGATTGCGGTTCAGGCCGCGCTCACCg                                        <  2:3754435/92‑1 (MQ=255)
                                                                    tACTTCGCCAGGACCCGGATGTCGTCATG‑GTGGG‑GGG‑AAATTCGTGATACAGAAACCGCGCAGATTGCGGTTCAGGCCTCGCTCACCGGCCATCTgg                               <  1:1419528/97‑1 (MQ=255)
                                                                    tACTTCGCCAGGACCCGGATGTCGTCATG‑GTGGG‑GGG‑AAATTCGTGATACAGAAACCGCGCAGATTGCGGTTCAGGCCTCGCTCACCGGCCATCTGGTAc                            <  2:3684701/100‑1 (MQ=255)
                                                                    tACTTCGCCAGGACCCGGATGTCGTCATG‑GTGGG‑GGG‑AAATTCGTGATACAGAAACCGCGCAGATTGCGGTTCAGGCCTCGCTCACCGGCCATCTGGTAc                            <  1:631834/100‑1 (MQ=255)
                                                                    tACTTCGCCAGGACCCGGATGTCGTCATG‑GTGGG‑GGG‑AAATTCGTGATACAGAAACCGCGCAGATTGCGGTTCAGACCTCGCTCACCGGCCATCTGGTAc                            >  1:874169/1‑100 (MQ=255)
                                                                      cTTCGCCAGGACCCGGATGTCGTCATG‑GTGGG‑GGG‑AAATTCGTGATACAGAAACCGCGCAGATTGCGGTTCAGGCCTCGCTCACCGGCCATCTGGTActc                          >  1:1801001/1‑100 (MQ=255)
                                                                      cTTCGCCAGGACCCGGATGTCGTCATG‑GTGGG‑GGG‑AAATTCGTGATACAGAAACCGCGCAGATTGCGGTTCAGGCCTCGCTCACCGGCCATCTGGTActc                          >  1:3119601/1‑100 (MQ=255)
                                                                      cTTCGCCAGGACCCGGATGTCGTCATG‑GTGGG‑GGG‑AAATTCGTGATACAGAAACCGCGCAGATTGCGGTTCAGGCCTCGCTCACCGGCCATCTGGTAcag                          >  1:2606930/1‑98 (MQ=255)
                                                                      cTTCGCCAGGACCCGGATGTCGTCATG‑GTGGG‑GGG‑AAATTCGTGATACAGAAACCGCGCAGATTGCGGATCAGGCCTCGCTCACCGGCCATCTGGTActc                          >  1:1086277/1‑100 (MQ=255)
                                                                       ttCGCCAGGACCCGGATGTCGTCATG‑GTGGG‑GGG‑AAATTCGTGATACAGAAACCGCGCAGATTGCGGTTCAGGCCTCGCTCACCGGCCATCTGGTActct                         <  2:1766440/100‑1 (MQ=255)
                                                                       ttCGCCAGGACCCGGATGTCGTCATG‑GTGGG‑GGG‑AAATTCGTGATACAGAAACCGCGCAGATTGCGGTACAGGCCTCGCTCACCGGCCATCTGGTActct                         <  2:552011/100‑1 (MQ=255)
                                                                         cGCCAGGACCCGGATGTCGTTATG‑GTGGG‑GGG‑AAATTCGTGATACAGAAACCGCGCAGATTGCGGTTCAGGCCTCGCTCACCGGCCATCTGGTACTCTCa                       <  1:3685930/100‑1 (MQ=255)
                                                                          gCCAGGACCCGGATGTCGTCATG‑GTGGG‑GGG‑AAATTCGTGATACAGAAACCACACAAATAGCAAATCAGGACTCGCTCACCGGCCATCTGGTACTCTCaa                      >  1:177384/1‑100 (MQ=255)
                                                                           ccAGGACCCGGATGTCGTCATGAAG‑GG‑GGG‑AAATTCGTGATACAGAAACCGCGCAGATTGCGGTTCAGGCCTCGCTCACCGGCCATCTGGTACTCTCAac                     >  2:1800319/1‑100 (MQ=255)
                                                                           ccAGGACCCGGATGTCGTCATG‑GTGGG‑GGG‑AAATTCGTGATACAGAAACCGCGCAGATTGCGGTTCAGGCCTCGCTCACCGGCCATCTGGTACTCTCAac                     <  1:2927895/100‑1 (MQ=255)
                                                                           ccAGGACCCGGATGTCGTCATG‑GTGGG‑GGG‑AAATTCGTGATACAGAAACCGCGCAGATTGCGGTTCAGGCCTCGCTCACCGGCCATCTGGTACTCTCAac                     <  1:2542879/100‑1 (MQ=255)
                                                                           ccAGGACCCGGATGTCGTCATG‑GTGGG‑GGG‑AAATTCGTGATACAGAAACCGCGCAGATTACAGATAAGACCTCGCTCACCGGCCATCTGGTACTCTCAac                     >  1:2331898/1‑100 (MQ=255)
                                                                            cAGGACCCGGATGTCGTCATG‑GTGGG‑GGG‑AAATTCGTGATACAGAAACCGCGCAGATTGCGGTTCAGGCCTCGCTCACCGGCCATCTGGTACTCTCAaca                    <  2:2705285/100‑1 (MQ=255)
                                                                            cAGGACCCGGATGTCGTCATG‑GTGGG‑GGG‑AAATTCGTGATACAGAAACCGCGCAGATGGCGGTTCAGGCCTCGCTCACCGGCCATCTGGTACTCTCAaca                    >  2:2183922/1‑100 (MQ=255)
                                                                              ggACCCGGATGTCGTCATG‑GTGGG‑GGG‑AAATTCGTGATACAGAAACCGCGCAGATTGCGGTTCAGGCCTCGCTCACCGGCCATCTGGTACTCTCAACACt                  >  1:601900/1‑100 (MQ=255)
                                                                              ggACCCGGATGTCGTCATG‑GTGGG‑GGG‑AAATTCGTGATACAGAAACCGCGCAGATTGCGGTTCAGGCCTCGCTCACCGGCCATCTGGTACTCTCAACACt                  >  1:2089070/1‑100 (MQ=255)
                                                                               gACCCGGATGTCGTCATG‑GT‑GG‑GGG‑AAATTCGTGATACAGAAACCGCGCAGATTGCGGTTCAGGCCTCGCTCACCGGCCATCTGGTACTCTCAACACTcc                <  2:2931611/100‑1 (MQ=255)
                                                                                 cccGGCTGTCGTCTTT‑GTGGG‑GGG‑AAATTCGTGATACAGAAATCGGGCAGATTGCGGTTCAGGCCTCGCTCACCGTCCATCTTGTACTCTCAACACTCca               >  2:640889/1‑100 (MQ=255)
                                                                                 cccGGATGTCGTCATG‑GTGGG‑GGG‑AAATTCGTGATACAGAAACCGCGCAGATTGCGGTTCAGGCCTCGCTCACCGGCCATCTGGTACTCTCAACACTCca               >  1:2749076/1‑100 (MQ=255)
                                                                                  ccGGATGTCGTCATG‑GTGGG‑GGG‑AAATTCGTTATACAGAAACCGCGCAGATTGCGGTTCAGGCCTCGCTCACCGGCCATCTGGTACTCTCAACACTCcac              <  1:1150478/100‑1 (MQ=255)
                                                                                  ccGGATGTCGTCATG‑GTGGG‑GGG‑AAATTCGTGATACAGAAACCGCGCAGATTGCGGTTCAGGCCTCGCTCACCGGCCATCTGGTACTCTCAACACTCcac              <  1:1458364/100‑1 (MQ=255)
                                                                                        gtcgtcATG‑GTGGG‑GGG‑AAATTCGTGATACAGAAACCGCGCAGATTGCGGTTCAGGCCTCGCTCACCGGCCATCTGGTACTCTCAACACTCCACACTAAc        <  2:712492/100‑1 (MQ=255)
                                                                                          cgtcATG‑GTGGG‑GGG‑AAATTCGTGATACAGAAACCGCGCAGATTGCGGTTCAGGCCTCGCTCACCGGCCATCTGGTACTCTCAACACTCCacac            <  2:801171/94‑1 (MQ=255)
                                                                                          cgtcATG‑GTGGG‑GGG‑AAATTCGTGATACAGAAACCGCGCAGATTGCGGTTCAGGCCTCGCTCACCGGCCATCTGGTACTCTCAACACTCCACACTAACAg      <  2:3664143/100‑1 (MQ=255)
                                                                                          cgtcATG‑GT‑GG‑GGGTAAATTCGTGATACAGAAACCGCGCAGATTGCGGTTCAGGCCTCGCTCACCGGCCATCTGGTACTCTCAACACTCCACACTAACAg      <  1:577252/100‑1 (MQ=255)
                                                                                            tcATG‑GTGGG‑GGG‑AAATTCGTGATACAGAAACCGCGCAGATTGCGGTTCAGGCCTCGCTCACCGGCCATCTGGTACTCTCAACACTCCACACTAACAGTg    >  2:2922826/1‑100 (MQ=255)
                                                                                              aTG‑GTGGG‑GGG‑AAATTCGTGATACAGAAACCGCGCAGATTGCGGTTCAGGCCTCGCTCACCGGCCATCTGGTACTCTCAACACTCCACACTAACAGTGc   <  2:2475946/99‑1 (MQ=255)
                                                                                              aTG‑GTGGG‑GGG‑AAATTCGTGATACAGAAACCGCGCAGATTGCGGTTCAGGCCTCGCTCACCGGCCATCTGGTACTCTCAACACTCCACACTAACAGTGCa  <  2:1552901/100‑1 (MQ=255)
                                                                                                    |                                                                                                
GGAAGGCATTGGGCAAACGCAGGTGAATACCCGTGTGGATATGTCTTTCGCTCGCGGCCTGCGCGCCATACTTCGCCAGGACCCGGATGTCGTCATG‑GT‑GG‑GGG‑AAATTCGTGATACAGAAACCGCGCAGATTGCGGTTCAGGCCTCGCTCACCGGCCATCTGGTACTCTCAACACTCCACACTAACAGTGCA  >  NC_000913/3459190‑3459382

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A