Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A9 F200 I0 R1 101 105.4 5209919 94.4% 4918163 99.8

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 4,128,197 T→C 100% T67A (ACC→GCC)  metJ ← transcriptional repressor, S‑adenosylmethionine‑binding

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,128,1970TC92.2% 379.5 / 13.8 123T67A (ACC→GCC) metJtranscriptional repressor, S‑adenosylmethionine‑binding
Reads supporting (aligned to +/- strand):  ref base T (6/4);  new base C (55/58);  total (61/62)
Fisher's exact test for biased strand distribution p-value = 5.30e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 123 total aligned reads displayed.

TAATCCCCATCTCACGCATGATCTCTTTTGCCGCTTCCGGGATTTCGTCGCTGCGCTCTTTACGCAGATCGGCATCATCCGGCAAAGGTTGCCCGGTAAAGGCATGCAGAAACGCTTCGCACAGCAGCTCGCTGTTGGTAGCGTGACGCAGGTTGTTCACCTGACGACGCGTGCGTTCATCGGTGAGGATTT  >  NC_000913/4128101‑4128292
                                                                                                |                                                                                               
tAATCCCCATCTCACGCATGATCTCTTTTGCCGCTTCCGGGATTTCGTCGCTGCGCTCTTTACGCAGATCGGCATCATCCGGCAAAGGTTGCCCGGCaaa                                                                                              >  1:1158816/1‑100 (MQ=255)
 aaTCCCCATCTCACGCATGATCTCTTTTGCCGCTTCCGGGATTTCGTCGCTGCGCTCTTTACGCAGATCGGCATCATCCGGCAAAGGTTGCCCGGCAAAg                                                                                             <  1:1193985/100‑1 (MQ=255)
 aaTCCCCATCTCACGCATGATCTCTTTTGCCGCTTCCGGGATTTCGTCGCTGCGCTCTTTACGCAGATCGGCATCATCCGGCAAAGGTTGCCCGGCAAAg                                                                                             <  1:884996/100‑1 (MQ=255)
 aaTCCCCATCTCACGCATGATCTCTTTTGCCGCTTCCGGGATTTCGTCGCTGCGCTCTTTACGCAGATCGGCATCATCCGGCAAAGGTTGCCCGGCAAAg                                                                                             <  2:1885355/100‑1 (MQ=255)
  aTCCCCATCTCACGCATGATCTCTTTTGCCGCTTCCGGGATTTCGTCGCTGCGCTCTTTACGCAGATCGGCATCATCCGGCAAAGGTTGCCCGGCAAAgg                                                                                            >  1:1900957/1‑100 (MQ=255)
  aTCCCCATCTCACGCATGATCTCTTTTGCCGCTTCCGGGATTTCGTCGCTGCGCTCTTTACGCAGATCGGCATCATCCGGCAAAGGTTGCCCGGCAAAgg                                                                                            <  1:1261154/100‑1 (MQ=255)
  aTCCCCATCTCACGCATGATCTCTTTTGCCGCTTCCGGGATTTCGTCGCTGCGCTCTTTACGCAGATCGGCATCATCCGGCAAAGGTTGCCCGGCAAAgg                                                                                            >  1:2267757/1‑100 (MQ=255)
  aTCCCCATCTCACGCATGATCTCTTTTGCCGCTTCCGGGATTTCGTCGCTGCGCTCTTTACGCAGATCGGCATCATCCGGCAAAGGTTGCCCGGCAAAgg                                                                                            >  1:1397614/1‑100 (MQ=255)
  aTCCCCATCTCACGCATGATCTCTTTTGCCGCTTCCGGGATTTCGTCGCTGCGCTCTTTACGCAGATCGGCATCATCCGGCAAAGGTTGCCCGGCAAAgg                                                                                            <  1:493341/100‑1 (MQ=255)
  aTCCCCATCTCACGCATGATCTCTTTTGCCGCTTCCGGGATTTCGTCGCTGCGCTCTTTACGCAGATCGGCATCATCCGGCAAAGGTTGCCCGGCAAAgg                                                                                            <  1:246550/100‑1 (MQ=255)
   tCCCCATCTCACGCATGATCTCTTTTGCCGCTTCCGGGATTTCGTCGCTGCGCTCTTTACGCAGATCGGCATCATCCGGCAAAGGTTGCCCGGCAAAGGc                                                                                           >  1:936422/1‑100 (MQ=255)
     cccATCTCACGCATGATCTCTTTTGCCGCTTCCGGGATTTCGTCGCTGCGCTCTTTACGCAGATCGGCATCATCCGGCAAAGGTTGCCCGGTAAAGGCAt                                                                                         >  1:1130196/1‑100 (MQ=255)
     cccATCTCACGCATGATCTCTTTTGCCGCTTCCGGGATTTCGTCGCTGCGCTCTTTACGCAGATCGGCATCATCCGGCAAAGGTTGCCCGGCAAAGGCAt                                                                                         >  1:1121212/1‑100 (MQ=255)
     cccATCTCACGCATGATCTCTTTTGCCGCTTCCGGGATTTCGTCGCTGCGCTCTTTACGCAGATCGGCATCATCCGGCAAAGGTTGCCCGGCAAAGGCAt                                                                                         >  1:2285305/1‑100 (MQ=255)
      ccATCTCACGCATGATCTCTTTTGCCGCTTCCGGGATTTCGTCGCTGCGCTCTTTACGCAGATCGGCATCATCCGGCAAAGGTTGCCCGGCAAAGGCATg                                                                                        <  2:202032/100‑1 (MQ=255)
       cATCTCACGCATGATCTCTTTTGCCGCTTCCGGGATTTCGTCGCTGCGCTCTTTACGCAGATCGGCATCATCCGGCAAAGGTTGCCCGGCAAAGGCATGc                                                                                       <  1:2492084/100‑1 (MQ=255)
         tctcACGCCTGATCTCTTTTGCCGCTTCCGGGATTTCGTCGCTGCGCTCTTTACGCAGATCGGCATCATCCGGCAAAGGTTGCCCGGCAAAGGCATGCAg                                                                                     <  1:2243081/100‑1 (MQ=255)
             aCGCATGATCTCTTTTGCCGCTTCCGGGATTTCGTCGCTGCGCTCTTTACGCAGATCGGCATCATCCGGCAAAGGTTGCCCGGCAAAGGCATGCAGAAAc                                                                                 <  1:1740220/100‑1 (MQ=255)
             aCGCATGATCTCTTTTGCCGCTTCCGGGATTTCGTCGCTGCGCTCTTTACGCAGATCGGCATCATCCGGCAAAGGTTGCCCGGCAAAGGCATGCAGAAAc                                                                                 >  1:2049520/1‑100 (MQ=255)
              cGCATGATCTCTTTTGCCGCTTCCGGGATTTCGTCGCTGCGCTCTTTACGCAGATCGGCATCATCCGGCAAAGGTTGCCCGGCAAAGGCATGCAGAAACg                                                                                >  1:476418/1‑100 (MQ=255)
              cGCATGATCTCTTTTGCCGCTTCCGGGATTTCGTCGCTGCGCTCTTTACGCAGATCGGCATCATCCGGCAAAGGTTGCCCGGCAAAGGCATGCAGAAACg                                                                                <  1:682202/100‑1 (MQ=255)
                cATGATCTCTTTTGCCGCTTCCGGGATTTCGTCGCTGCGCTCTTTACGCAGATCGGCATCATCCGGCAAAGGTTGCCCGGCAAAGGCATGCAGAAACGCt                                                                              >  1:747803/1‑100 (MQ=255)
                  tGATCTCTTTTGCCGCTTCCGGGATTTCGTCGCTGCGCTCTTTACGCAGATCGGCATCATCCGGCAAAGGTTGCCCGGCAAAGGCATGCAGAAACGCTTc                                                                            <  2:1106738/100‑1 (MQ=255)
                   gATCTCTTTTGCCGCTTCCGGGATTTCGTCGCTGCGCTCTTTACGCAGATCGGCATCATCCGGCAAAGGTTGCCCGGCAAAGGCATGCAGAAACGCTTCg                                                                           >  2:140678/1‑100 (MQ=255)
                   gATCTCTTTTGCCGCTTCCGGGATTTCGTCGCTGCGCTCTTTACGCAGATCGGCATCATCCGGCAAAGGTTGCCCGGCAAAGGCATGCAGAAACGCTTCg                                                                           >  2:2464319/1‑100 (MQ=255)
                    aTCTCTTTTGCCGCTTCCGTTTTTTTTTCACTGCGCTCTTTACGCAGATCGGCATCATCCGGCAAAGGTTGACCGGCAAAGGCATGCAGataatcttc                                                                            >  2:220355/1‑90 (MQ=255)
                    aTCTCTTTTGCCGCTTCCGGGATTTCGTCGCTGCGCTCTTTACGCAGATCGGCATCATCCGGCAAAGGTTGCCCGGCAAAGGCATGCAGAAACGCTTCGc                                                                          >  2:2077157/1‑100 (MQ=255)
                      ctctTTTGCCGCTTCCGGGATTTCGTCGCTGCGCTCTTTACGCAGATCGGCATCATCCGGCAAAGGTTGCCCGGCAAAGGCATGCAGAAACGCTTCGcac                                                                        <  2:1113766/100‑1 (MQ=255)
                      ctctTTTGCCGCTTCCGGGATTTCGTCGCTGCGCTCTTTACGCAGATCGGCATCATCCGGCAAAGGTTGCCCGGCAAAGGCATGCAGAAACGCTTCGcac                                                                        <  1:2371008/100‑1 (MQ=255)
                       tctTTTGCCGCTTCCGGGATTTCGTCGCTGCGCTCTTTACGCAGATCGGCATCATCCGGCAAAGGTTGCCCGGCAAAGGCATGCAGAAACGCTTCGcaca                                                                       >  2:790487/1‑100 (MQ=255)
                       tctTTTGCCGCTTCCGGGATTTCGTCGCTGCGCTCTTTACGCAGATCGGCATCATCCGGCAAAGGTTGCCCGGCAAAGGCATGCAGAAACGCTTCGcaca                                                                       >  2:1124935/1‑100 (MQ=255)
                        ctTTTGCCGCTTCCGGGATTTCGTCGCTGCGCTCTTTACGCAGATCGGCATCATCCGGCAAAGGTTGCCCGGCAAAGGCATGCAGAAACGCTTCGCAcag                                                                      >  1:202847/1‑100 (MQ=255)
                         ttttGCCGCTTCCGGGATTTCGTCGCTGCGCTCTTTACGCAGATCGGCATCATCCGGCAAAGGTTGCCCGGCAAAGGCATGCAGAAACGCTTCGCAcagc                                                                     <  2:2350568/100‑1 (MQ=255)
                             gCCGCTTCCGGGATTTCGTCGCTGCGCTCTTTACGCCGATCGGCATCATCCGGCAAAGGTTGCCCGGCAAAGGCATGCAGAAACGCTTCGCACAGCAGCt                                                                 >  2:2442969/1‑100 (MQ=255)
                             gCCGCTTCCGGGATTTCGTCGCTGCGCTCTTTACGCAGATCGGCATCATCCGGCAAAGGTTGCGCGGCAAAGGCATGAAGAAACGCTTTGCACGGCAGCt                                                                 >  2:127168/1‑100 (MQ=255)
                             gCCGCTTCCGGGATTTCGTCGCTGCGCTCTTTACGCAGATCGGCATCATCCGGCAAAGGTTGCCCGGCAAAGGCATGCAGAAACGCTTCGCACAGCAGCt                                                                 >  2:1935260/1‑100 (MQ=255)
                              ccGCTTCCGGGATTTCGTCGCTGCGCTCTTTACGCAGATCGGCATCATCCGGCAAAGGTTGCCCGGCAAAGGCATGCAGAAACGCTTCGCACAGCAGCTc                                                                >  1:2149941/1‑100 (MQ=255)
                                  ttCCGGGATTTCGTCGCTGCGCTCTTTACGCAGATCGGCATCATCCGGCAAAGGTTGCCCGGTAAAGGCATGCAGAAACGCTTCGCACAGCAGCTCGCTg                                                            >  2:1684770/1‑100 (MQ=255)
                                   tCCGGGATTTCGTCGCTGCGCTCTTTACGCAGATCGGCATCATCCGGCAAAGGTTGCCCGGCAAAGGCATGCAGAAACGCTTCGCACAGCAGCTCGCTGt                                                           >  1:161921/1‑100 (MQ=255)
                                    ccGGGATTTCGTCGCTGCGCTCTTTACGCAGATCGGCATCATCCGGCAAAGGTTGCCCGGCAAAGGCATGCAGAAACGCTTCGCACAGCAGCTCGCTGtt                                                          <  1:545112/100‑1 (MQ=255)
                                    ccGGGATTTCGTCGCTGCGCTCTTTACGCAGATCGGCATCATCCGGCAAAGGTTGCCCGGCAAAGGCATGCAGAAACGCTTCGCACAGCAGCTCGCTGtt                                                          <  1:1156021/100‑1 (MQ=255)
                                     cGGGATTTCGTCGCTGCGCTCTTTACGCAGATCGGCATCATCCGGCAAAGGTTGCCCGGCAAAGGCATGCAGAAACGCTTCGCACAGCAGCTCGCTGTTg                                                         <  2:2296598/100‑1 (MQ=255)
                                      gggATTTCGTCGCTGCGCTCTTTACGCAGATCGGCATCATCCGGCAAAGGTTGCCCGGCAAAGGCATGCAGAAACGCTTCGCACAGCAGCTCGCTGTTgg                                                        <  2:858889/100‑1 (MQ=255)
                                      gggATTTCGTCGCTGCGCTCTTTACGCAGATCGGCATCATCCGGCAAAGGTTGCCCGGCAAAGGCATGCAGAAACGCTTCGCACAGCAGCTCGCTGTTgg                                                        <  1:911168/100‑1 (MQ=255)
                                      gggATTTCGTCGCTGCGCTCTTTACGCAGATCGGCATCATCCGGCAAAGGTTGCCCGGCAAAGGCATGCAGAAACGCTTCGCACAGCAGCTCGCTGTTgg                                                        >  2:2273563/1‑100 (MQ=255)
                                       ggATTTCGTCGCTGCGCTCTTTACGCAGATCGGCATCATCCGGCAAAGGTTGCCCGGCAAAGGCATGCAGAAACGCTTCGCACGGCAGCTCGCTGTTGGt                                                       >  2:1949985/1‑100 (MQ=255)
                                       ggATTTCGTCGCTGCGCTCTTTACGCAGATCGGCATCATCCGGCAAAGGTTGCCCGGCAAAGGCATGCAGAAACGCTTCGCACAGCAGCTCGCTGTTGGt                                                       <  2:340952/100‑1 (MQ=255)
                                         aTTTCGTCGCTGCGCTCTTTACGCAGATCGGCATCATCCGGCAAAGGTTGCCCGGCAAAGGCATGCAGAAACGCTTCGCACAGCAGCTCGCTGTTGGTAg                                                     <  2:1721905/100‑1 (MQ=255)
                                          tttCGTCGCTGCGCTCTTTACGCAGATCGGCATCATCCGGCAAAGGTTGCCCGGCAAAGGCATGCAGAAACGCTTCGCACAGCAGCTCGCTGTTGGTAGc                                                    >  2:1682499/1‑100 (MQ=255)
                                          tttCGTCGCTGCGCTCTTTACGCAGATCGGCATCATCCGGCAAAGGTTGCCCGGCAAAGGCATGCAGAAACGCTTCGCACAGCAGCTCGCTGTTGGTAGc                                                    <  1:1554931/100‑1 (MQ=255)
                                          tttCGTCGCTGCGCTCTTTACGCAGATCGGCATCATCCGGCAAAGGTTGCCCGGCAAAGGCATGCAGAAACGCTTCGCACAGCAGCTCGCTGTTGGTAGc                                                    <  1:468721/100‑1 (MQ=255)
                                          tttCGTCGCTGCGCTCTTTACGCAGATCGACATCATCCGGCAAAGGTTGCCCGGCAAAGGCATGCAGAAACGCTTCGCACAGCAGCTCGCTGTTGGTGGc                                                    >  1:1823234/1‑100 (MQ=255)
                                           ttCGTCGCTGCGCTCTTTACGCAGATCGGCATCATCCGGCAAAGGTTGCCCGGCAAAGGCATGCAGAAACGCTTCGCACAGCAGCTCGCTGTTGGTAGCg                                                   <  2:476414/100‑1 (MQ=255)
                                             cgtcgCTGCGCTCTTTACGCAGATCGGCATCATCCGGCAAAGGTTGCCCGGCAAAGGCATGCAGAAACGCTTCGCACAGCAGCTCGCTGTTGGTAGCGTg                                                 <  2:846640/100‑1 (MQ=255)
                                              gtggCTGCGCTCTTTACGCAGATCGGGATCATCCGGCAAAGGTTGCGCGGCAAAGGCGTACAGAAAGGCTTAGCACAGCAGCTCGCTGTTGGTAGCGTg                                                 >  2:1791865/4‑99 (MQ=255)
                                               tcgCTGCGCTCTTTACGCAGATCGGCATCATCCGGCAAAGGTTGCCCGGCAAAGGCATGCAGAAACGCTTCGCACAGCAGCTCGCTGTTGGTAGCGTGAc                                               <  2:188370/100‑1 (MQ=255)
                                                cgCTGCGCTCTTTACGCAGATCGGCATCATCCGGCAAAGGTTGCCCGGCAAAGGCATGCAGAAACGCTTCGCACAGCAGCTCGCTGTTGGTAGCGTGACg                                              <  1:813688/100‑1 (MQ=255)
                                                 gCTGCGCTCTTTACGCAGATCGGCATCATCCGGCAAAGGTTGCCCGGCAAAGGCATGCAGAAACGCTTCGCACAGCAGCTCGCTGTTGGTAGCGTGACGc                                             >  2:908777/1‑100 (MQ=255)
                                                   tGCGCTCTTTACGCAGATCGGCATCATCCGGCAAAGGTTGCCCGGCAAAGGCATGCAGAAACGCTTCGCACAGCAGCTCGCTGTTGGTAGCGTGACGCAg                                           >  1:1778546/1‑100 (MQ=255)
                                                    gcgcTCTTTACGCAGATCGGCATCATCCGGCAAAGGTTGCCCGGCAAAGGCATGCAGAAACGCTTCGCACAGCAGCTCGCTGTTGGTAGCGTGACGCAgg                                          <  2:2395296/100‑1 (MQ=255)
                                                    gcgcTCTTTACGCAGATCGGCATCATCCGGCAAAGGTTGCCCGGCAAAGGCATGCAGAAACGCTTCGCACAGCAGCTCGCTGTTGGTAGCGTGACGCAgg                                          >  1:718776/1‑100 (MQ=255)
                                                     cgcTCTTTACGCAGATCGGCATCATCCGGCAAAGGTTGCCCGGCAAAGGCATGCAGAAACGCTTCGCACAGCAGCTCGCTGTTGGTAGCGTGACGCAGgt                                         <  1:1842690/100‑1 (MQ=255)
                                                     cgcTCTTTACGCAGATCGGCATCATCCGGCAAAGGTTGCCCGGCAAAGGCATGCAGAAACGCTTCGCACAGCAGCTCGCTGTTGGTAGCGTGACGCAGgt                                         >  1:1632352/1‑100 (MQ=255)
                                                       ctctTTACGCCGATCGGCATCATCCGGCAAAGGTTTCCCGGCAAAGGCATGCAGAAACGCTTCGCACAGCAGCTCGCTGTTGGTAGCGTGACGCAGgt                                         >  2:1455029/1‑98 (MQ=255)
                                                       ctctTTACGCAGATCGGCATCATCCGGCCAAGGTTGCCCGGCAAAGGCATGCAGAAACGCTTCGCACAGCAGCTCGCTGTTGGTAGCGTGACGCAGgttg                                       >  2:1455030/1‑100 (MQ=255)
                                                        tctTTACGCAGATCGGCATCATCCGGCAAAGGTTGCCCGGCAAAGGCATGCAGAAACGCTTCGCACAGCAGCTCGCTGTTGGTAGCGTGACGCAGgttgt                                      <  1:1269634/100‑1 (MQ=255)
                                                         ctTTACGCAGATCGGCATCATCCGGCAAAGGTTGCCCGGTAAAGGCATGCAGAAACGCTTCGCACAGCAGCTCGCTGTTGGTAGCGTGACGCAGgttgtt                                     >  1:112850/1‑100 (MQ=255)
                                                          tttACGCAGATCGGCATCATCCGGCAAAGGTTGCCCGGTAAAGGCATGCAGAAACGCTTCGCACAGCAGCTCGCTGTTGGTAGCGTGACGCAGGTTGTTc                                    <  2:112849/100‑1 (MQ=255)
                                                             aCGCAGATCGGCATCATCCGGCAAAGGTTGCCCGGCAAAGGCATGCAGAAACGCTTCGCACAGCAGCTCGCTGTTGGTAGCGTGACGCAGGTTGTTc                                    <  2:807813/97‑1 (MQ=255)
                                                             aCGCAGATCGGCATCATCCGGCAAAGGTTGCCCGGCAAAGGCATGCAGAAACGCTTCGCACAGCAGCTCGCTGTTGGTAGCGTGACGCAGGTTGTTCAcc                                 <  1:2333556/100‑1 (MQ=255)
                                                             aCGCAGATCGGCATCATCCGGCAAAGGTTGCCCGGCAAAGGCATGCAGAAACGCTTCGCACAGCAGCTCGCTGTTGGTAGCGTGACGCAGGTTGTTCAcc                                 <  1:2183231/100‑1 (MQ=255)
                                                              cGCAGATCGGCATCATCCGGCAAAGGTTGCCCGGCAAAGGCATGCAGAAACGCTTCGCACAGCAGCTCGCTGTTGGTAGCGTGACGCAGGTTGTTCACCt                                >  1:901240/1‑100 (MQ=255)
                                                              cGCAGATCGGCATCATCCGGCAAAGGTTGCCCGGCAAAGGCATGCAGAAACGCTTCGCACAGCAGCTCGCTGTTGGCAGCGTGACGCAGGTTGTTCAcc                                 <  2:1508361/99‑1 (MQ=255)
                                                                  gATCGGCATCATCCGGCAAAGGTTGCCCGGTAAAGGCATGCAGAAACGCTTCGCACAGCAGCTCGCTGTTGGTAGCGTGACGCAGGTTGTTCACCTgacg                            <  2:89004/100‑1 (MQ=255)
                                                                  gATCGGCATCATCCGGCAAAGGTTGCCCGGCAAAGGCATGCAGAAACGCTTCGCACAGCAGCTCGCTGTTGGTAGCGTGACGCAGGTTGTTCACCTgacg                            >  2:2088904/1‑100 (MQ=255)
                                                                   aTCGGCATCATCCGGCAAAGGTTGCCCGGCAAAGGCATGCAGAAACGCTTCGCACAGCAGCTCGCTGTTGGTAGCGTGACGCAGGTTGTTCACCTgacga                           <  2:2042437/100‑1 (MQ=255)
                                                                    tCGGCATCATCCGGCAAAGGTTGCCCGGCAAAGGCATGCAGAAACGCTTCGCACAGCAGCTCGCTGTTGGTAGCGTGACGCAGGTTGTTCACCTgacgac                          >  2:1240172/1‑100 (MQ=255)
                                                                     cGGCATCATCCGGCAAAGGTTGCCCGGCAAAGGCATGCAGAAACGCTTCGCACAGCAGCTCGCTGTTGGTAGCGTGACGCAGGTTGTTCACCTGacgacg                         <  1:2077168/100‑1 (MQ=255)
                                                                        catcatCCGGCAAAGGTTGCCCGGTAAAGGCATGCAGAAACGCTTCGCACAGCAGCTCGCTGTTGGTAGCGTGACGCAGGTTGTTCACCTGACGACgcgt                      >  1:2163439/1‑100 (MQ=255)
                                                                         atcatcCGGCAAAGGTTGCCCGGCAAAGGCATGCAGAAACGCTTCGCACAGCAGCTCGCTGTTGGTAGCGTGACGCAGGTTGTTCACCTgacgagatcgg                     >  1:2009511/1‑94 (MQ=255)
                                                                          tcatcCGGCAAAGGTTGCCCGGCAAAGGCATGCAGAAACGCTTCGCACAGCAGCTCGCTGTTGGTAGCGTGACGCAGGTTGTTCACCTGACGACgcgtgc                    <  2:1420045/100‑1 (MQ=255)
                                                                          tcatcCGGCAAAGGTTGCCCGGCAAAGGCATGCAGAAACGCTTCGCACAGCAGCTCGCTGTTGGTAGCGTGACGCAGGTTGTTCACCTGACGACgcgtgc                    <  2:1420172/100‑1 (MQ=255)
                                                                           catcCGGCAAAGGTTGCCCGGTAAAGGCATGCAGAAACGCTTCGCACAGCAGCTCGCTGTTGGTAGCGTGACGCAGGTTGTTCACCTGACGACgcgtgcg                   >  1:2592098/1‑100 (MQ=255)
                                                                           catcCGGCAAAGGTTGCCCGGCAAAGGCATGCAGAAACGCTTCGCACAGCAGCTCGCTGTTGGTAGCGTGACGCAGGTTGTTCACCTGACGACgcgtgcg                   >  1:1908104/1‑100 (MQ=255)
                                                                             tcCGGCAAAGGTTGCCCGGCAAAGGCATGCAGAAACGCTTCGCACAGCAGCTCGCTTTTGGTAGCGTGACGCAGGTTGTTCACCTGACGACGCGTGCGtt                 <  1:1273259/100‑1 (MQ=255)
                                                                             tcCGGCAAAGGTTGCCCGGCAAAGGCATGCAGAAACGCTTCGCACAGCAGCTCGCTGTTGGTAGCGTGACGCAGGTTGTTCACCTGACGACGCGTGCGtt                 >  1:527894/1‑100 (MQ=255)
                                                                               cGGCAAAGGTTGCCCGGCAAAGGCATGCAGAAACGCTTCGCACAGCAGCTCGCTGTTGGTAGCGTGACGCAGGTTGTTCACCTGACGACgcgtgcttttg               >  1:1771522/1‑95 (MQ=255)
                                                                               cGGCAAAGGTTGCCCGGCAAAGGCATGCAGAAACGCTTCGCACAGCAGCTCGCTGTTGGTAGCGTGACGCAGGTTGTTCACCTGACGACGCGTGCGTTCa               <  1:84879/100‑1 (MQ=255)
                                                                                   aaaGGTTGCCCGGCAAAGGCATGCAGAAACGCTTCGCACAGCAGCTCGCTGTTGGTAGCGTGACGCAGGTTGTTCACCTGACGACGCGTGCGTTCATCgg           <  1:527104/100‑1 (MQ=255)
                                                                                    aaGGTTGCCCGGCAAAGGCATGCAGAAACGCTTCGCACAGCAGCTCGCTGTTGGTAGCGTGACGCAGGTTGTTCACCTGACGACGCGTGCGTTCATCGGt          <  1:444853/100‑1 (MQ=255)
                                                                                     aGGTTGCCCGGCAAAGGCATGCAGAAACGCTTCGCACAGCAGCTCGCTGTTGGTAGCGTGACGCAGGTTGTTCACCTGACGACGCGTGCGTTCATCGGTg         <  2:611051/100‑1 (MQ=255)
                                                                                       gTTGCCCGGTAAAGGCATGCAGAAACGCTTCGCACAGCAGCTCGCTGTTGGTAGCGTGACGCAGGTTGTTCACCTGACGACGCGTGCGTTCATCGGTGAg       <  1:1320245/100‑1 (MQ=255)
                                                                                       gTTGCCCGGCAAAGGCATGCAGAAACGCTTCGCACAGCAGCTCGCTGTTGGTAGCGTGACGCAGGTTGTTCACCTGACGACGCGTGCGTTCATCGGTGAg       <  1:321782/100‑1 (MQ=255)
                                                                                       gTTGCCCGGCAAAGGCATGCAGAAACGCTTCGCACAGCAGCTCGCTGTTGGTAGCGTGACGCAGGTTGTTCACCTGACGACGCGTGCGTTCATCGGTGAg       <  2:2145993/100‑1 (MQ=255)
                                                                                       gTTGCCCGGCAAAGGCATGCAGAAACGCTTCGCACAGCAGCTCGCTGTTGGTAGCGTGACGCAGGTTGTTCACCTGACGACGCGTGCGTTCATCGGTGAg       >  1:2498939/1‑100 (MQ=255)
                                                                                         tGCCCGGCAAAGGCATGCAGAAACGCTTCGCACAGCAGCTCGCTGTTGGTAGCGTGACGCAGGTTGTTCACCTGACGACGCGTGCGTTCATCGGTGAGGa     >  2:1158536/1‑100 (MQ=255)
                                                                                          gCCCGGCAAAGGCATGCAGAAACGCTTCGCACAGCAGCTCGCTGTTGGTAGCGTGACTCAGGTTGTTCACCTGACGACGCGTGCGTTCATCGGTGAggct    <  1:1762071/100‑3 (MQ=255)
                                                                                          gCCCGGCAAAGGCATGCAGAAACGCTTCGCACAGCAGCTCGCTGTTGGTAGCGGGACGCAGGTTGTGCACCTGACGACGCGTGCGTTCATCGGTGAGGa     >  1:2074776/1‑99 (MQ=255)
                                                                                           cccGGCAAAGGCATGCAGAAACGCTTCGCACAGCAGCTCGCTGTTGGTAGCGTGACGCAGGTTGTTCACCTGACGACGCGTGCGTTCATCGGTGAGGAtt   >  2:1189013/1‑100 (MQ=255)
                                                                                            ccGGTAAAGGCATGCAGAAACGCTTCGCACAGCAGCTCGCTGTTGGTAGCGTGACGCAGGTTGTTCACCTGACGACGCGTGCGTTCATCGGTGAGGAttt  >  1:47769/1‑100 (MQ=255)
                                                                                                |                                                                                               
TAATCCCCATCTCACGCATGATCTCTTTTGCCGCTTCCGGGATTTCGTCGCTGCGCTCTTTACGCAGATCGGCATCATCCGGCAAAGGTTGCCCGGTAAAGGCATGCAGAAACGCTTCGCACAGCAGCTCGCTGTTGGTAGCGTGACGCAGGTTGTTCACCTGACGACGCGTGCGTTCATCGGTGAGGATTT  >  NC_000913/4128101‑4128292

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A