Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A16 F201 I0 R1 306 176.9 8831342 93.4% 8248473 99.8

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 4,128,197 T→C 25.4% T67A (ACC→GCC)  metJ ← transcriptional repressor, S‑adenosylmethionine‑binding

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,128,1970TC25.4% 277.8 / 117.2 177T67A (ACC→GCC) metJtranscriptional repressor, S‑adenosylmethionine‑binding
Reads supporting (aligned to +/- strand):  ref base T (62/70);  new base C (21/24);  total (83/94)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.20e-02

Only 100 of 180 total aligned reads displayed.

GTTAATCCCCATCTCACGCATGATCTCTTTTGCCGCTTCCGGGATTTCGTCGCTGCGCTCTTTACGCAGATCGGCATCATCCGGCAAAGGTTGCCCGGTAAAGGCATGCAGAAACGCTTCGCACAGCAGCTCGCTGTTGGTAGCGTGACGCAGGTTGTTCACCTGACGACGCGTGCGTTCATCGGTGAGGATTTTTAA  >  NC_000913/4128099‑4128296
                                                                                                  |                                                                                                   
gTTAATCCCCATCTCACGCATGATCTCTTTTGCCGCTTCCGGGATTTCGTCGCTGCGCTCTTTACGCAGATCGGCATCATCCGGCAAAGGTTGCCCGGTa                                                                                                    <  2:3522355/100‑1 (MQ=255)
 ttAATCCCCATCTCACGCATGATCTCTTTTGCCGCTTCCGGGATTTCGTCGCTGCGCTCTTTACGCAGATCGGCATCATCCGGCAAAGGTTGCCCGGCaa                                                                                                   <  1:1151988/100‑1 (MQ=255)
  tAATCCCCATCTCACGCATGATCTCTTTTGCCGCTTCCGGGATTTCGTCGCTGCGCTCTTTCTGCAGATCGGCATCATCCGGCAAAGGTTGCCCGGTaaa                                                                                                  >  1:195664/1‑100 (MQ=255)
  tAATCCCCATCTCACGCATGATCTCTTTTGCCGCTTCCGGGATTTCGTCGCTGCGCTCTTTACGCAGATCGGCATCATCCGGCAAAGGTTGCCCGGTaaa                                                                                                  <  1:2150835/100‑1 (MQ=255)
   aaTCCCCATCTCACGCATGATCTCTTTTGCCGCTTCCGGGATTTCGTCGCTGCGCTCTTTACGCAGATCGACATCATCCGGAAAAGGTTGCCCGGTaaa                                                                                                  >  1:83568/1‑99 (MQ=255)
    aTCCCCATCTCACGCATGATCTCTTTTGCCGCTTCCGGGATTTCGTCGCTGCGCTCTTTACGCAGATCGGCATCATCCGGCAAAGGTTGCCCGGTAAAgg                                                                                                <  1:2046594/100‑1 (MQ=255)
      ccccATCTCACGCATGATCTCTTTTGCCGCTTCCGGGAGTTCGTCGCTGCGCTCTTTACGCAGATCGGCATCATCCGGCAAAGGTTGCCCGGTAAAGGCa                                                                                              <  1:3179924/100‑1 (MQ=255)
       cccATCTCACGCATGATCTCTTTTGCCGCTTCCGGGATTTCGTCGCTGCGCTCTTTACGCAGATCGGCATCATCCGGCAAAGGTTGCCCGGTAAAGGCAt                                                                                             <  2:4274817/100‑1 (MQ=255)
       cccATCTCACGCATGATCTCTTTTGCCGCTTCCGGGATTTCGTCGCTGCGCTCTTTACGCAGATCGGCATCATCCGGCAAAGGTTGCCCGGCAAAGGCAt                                                                                             >  1:754102/1‑100 (MQ=255)
        ctaTCTCACGCATGATCTCTTTTGCCGCTTCCGGGATTTCGTCGCTGCGCTCTTTACGCAGATCGGCATCATCCGGCAAAGGTTGCCCGGTAAAGGCATg                                                                                            <  1:2060888/98‑1 (MQ=255)
        ccATCTCACGCATGATCTCTTTTGCCGCTTCCGGGATTTCGTCGCTGCGCTCTTTACGCAGATCGGCATCATCCGGCAAAGGTTGCCCGGCAAAGGCATg                                                                                            <  1:1612667/100‑1 (MQ=255)
        ccATCTCACGCATGATCTCTTTTGCCGCTTCCGGGATTTCGTCGCTGCGCTCTTTACGCAGATCGGCATCATCCGCCAAAGGTTGCCCGGTCAAGGCa                                                                                              >  1:1170663/1‑98 (MQ=255)
         cATCTCTCGCATGATCTCTTTTGCCGCGTCCGGGATTTCGTCGCTGCGCTCTTTACGCAGATCGGCATCATCCGGCAAAGGTTGCCCGGTAAAGGCa                                                                                              <  1:858001/97‑1 (MQ=255)
          aTCTCACGCATGATCTCTTTTGCCGCTTCCGGGATTTCGTCGCTGCGCTCTTTACGCAGATCGGCATCATCCGGCAAAGGTTGCCCGGTAAAGGCATgag                                                                                          >  2:2060895/1‑98 (MQ=255)
            ctcACGCATGATCTCTTTTGCCGCTTCCGGGATTTCGTCGCTGCGCTCTTTACGCAGATCGGCATCATCCGGCAATGGTTGCCCGGTAAAGGCATGCAGa                                                                                        >  2:3970472/1‑100 (MQ=255)
             tcACGCATGATCTCTTTTGCCGCTTCCGGGATTTCGTCGCTGCGCTCTTTACGCAGATCGGCATCATCCGGCAAAGGTTGCCCGGTAAAGGCATGCAGaa                                                                                       >  1:319243/1‑100 (MQ=255)
             tcACGCATGATCTCTTTTGCCGCTTCCGGGATTTCGTCGCTGCGCTCTTTACGCAGATCGGCATCATCCGGCAAAGGTTGCCCGGCAAAGGCATGCAg                                                                                         <  1:2677877/98‑1 (MQ=255)
             ncACGCATGATCTCTTTTGCCGCTTCCGGGATTTCGTCGCTGCGCTCTTTACGCAGATCGGCATCATCCGGCAAAGGTTGCCCGGTAAAGGCATGCAGaa                                                                                       >  1:2365288/2‑100 (MQ=255)
                cGCATGATCTCTTTTGCCGCTTCCGGGATTTCGTCGCTGCGCTCTTTACGCAGATCGGCATCATCCGGCAAAGGTTGCCCGGTAAAGGCATGCAGAAACg                                                                                    >  1:4064036/1‑100 (MQ=255)
                cGCATGATATCTTTTGCCGCTTACGGGATTTCGACGCTGCGCTCTTTACGCAGATTGGCATCATTCGGCTAAGGTTGCGCGGCAAAGGCATGCAGAAAc                                                                                     >  1:3433197/1‑99 (MQ=255)
                   aTGATCTCTTTTGCCGCTTCCGGGATTTCGTCGCTGCGCTCTTTACGCAGATCGGCATCATCCGGCAAAGGTTGCCCGGCAAAGGCATGCAGAAACGCtt                                                                                 <  1:1610698/100‑1 (MQ=255)
                    tGATCTCTTTTGCCGCTTCCGGGATTTCGTCGCTGCGCTCTTTACGCAGATCGGCATCATCCGGCAAAGGTTGCCCGGTAAAGGCATGCAGAAACGCTTc                                                                                >  1:4309403/1‑100 (MQ=255)
                     gATCTCTTTTGCCGCTTCCGGGATTTCGTCGCTGCGCTCTTTACGCAGATCGGCATCATCCGGCAAAGGTTGCCCGGTAAAGGCATGCAGAAACGCTTCg                                                                               >  2:884568/1‑100 (MQ=255)
                     gATCTCTTTTGCCGCTTCCGGGATTTCGTCGCTGCGCTCTTTACGCAGATCGGCATCATCCGGCAAAGGTTGCCCGGCAAAGGCATGCAGAAACGCTTCg                                                                               >  2:1687740/1‑100 (MQ=255)
                     gATCTCTTTTGCCGCTCCCGGGATGTCGTCGCTGCGCTCTTTCCCCAGATCAGCATCATCCGGCCAAGGTTCCCCGGTAAAGGCCTGCAGAAACGccac                                                                                <  1:3082398/99‑4 (MQ=255)
                      aTCTCTTTTGCCGCTTCCGGGATTTCGTCGCTGCGCTCTTTACGCAGATCGGCATCATCCGGCAAAGGTTGCCCGGTAAAGGCATGCAGAAACGCTTCGc                                                                              <  2:944315/100‑1 (MQ=255)
                      aTCTCTTTTGCCGCTTCCGGGATTTCGTCGCTGCGCTCTTTACGCAGATCGGCATCATCCGGCAAAGGTTGCCCGGCAAAGGCATGCAGAAACGCTTCGc                                                                              <  1:1413692/100‑1 (MQ=255)
                      aTCTCTTTTGCCGCTTCCGGGATTTCGTCGCTGCGCTCTTTACGCAGATCGGCATCATCCGGCAAAGGTTGCCCGGCAAAGGCATGCAGAAACGCTTCGc                                                                              >  2:1347851/1‑100 (MQ=255)
                        ctctTTTGCCGCTTCCGGGATTTCGTCGCTGCGCTCTTTACGCAGATCGGCATCATCCGGCAAAGGTTGCCCGGTAAAGGCATGCAGAAACGCTTCGcac                                                                            >  1:2446823/1‑100 (MQ=255)
                             ttGCCGCTTCCGGGATTTCGTCGCTGCGCTCTTTACGCAGATCGGCATCATCCGGCAAAGGTTGCCCGGTAAAGGCATGCAGAAACGCTTCGCAcagcag                                                                       >  2:3172784/1‑100 (MQ=255)
                              tGCCGCTTCCGGGATTTCGTCGCTGCGCTCTTTACGCAGATCGGCATCATCCGGCAAAGGTTGCCCGGTAAAGGCATGCAGAAACGCTTCGCACagcagc                                                                      >  1:2645854/1‑100 (MQ=255)
                              tGCCGCTTCCGGGATTTCGTCGCTGCGCTCTGTACGCAGATCGGCATCATCCGGCAAAGGTTGCCCGGTAAAGGCATGCAGAAACGCTTCGCACagcagc                                                                      <  1:422593/100‑1 (MQ=255)
                               gCCGCTTCCGGGATCTCGTCGCTGCGCTCTGTACGCAGATCGGCATCAGCCGGCAAAGGTGACCCGGCAAAGGCATGCAGAAACGCTTCGCACAGCAGCt                                                                     <  1:2729144/100‑1 (MQ=255)
                                 cGCTTCCGGGATTTCGTCGCTGCGCTCTTTACGCAGATCGGCATCATCCGGCAAAGGTTGCCCGGTAAAGGCATGCAGAAACGCTTCGCACAGCAGCTCg                                                                   <  1:3858819/100‑1 (MQ=255)
                                  gCTTCCGGGATTTCGTCGCTGCGCTCTTTACGCAGATCGGCATCATCCGGCAAAGGTTGCCCGGTAAAGGCATGCAGAAACGCTTCGCACAGCAGCTCGc                                                                  <  2:2854101/100‑1 (MQ=255)
                                  gCTTCCGGGATTTCGTCGCTGCGCTCTTTACGCAGATCGGCATCATCCGGCAAAGGTTGCCCGGTAAAGGCATGCAGAAACGCTTCGCACAGCAGCTCGc                                                                  <  1:1914687/100‑1 (MQ=255)
                                     tCCGGGATTTCGTCGCTGCGCTCTTTACGCAGATCGGCATCATCCGGCAAAGGTTGCCCGGCAAAGGCATGCAGAAACGCTTCGCACAGCAGCTCGCTGt                                                               <  2:3572625/100‑1 (MQ=255)
                                       cGGGATTTCGTCGCTGCGCTCTTTACGCAGATCGGCATCATCCGGCAAAGGTTGCCCGGTAAAGGCATGCAGAAACGCTTCGCACAGCAGCTCGCTGTTg                                                             <  2:3651452/100‑1 (MQ=255)
                                       cGGGATTTCGTCGCTGCGCTCTTTACGCAGATCGGCATCATCCGGCAAAGGTTGCCCGGTAAAGGCATGCAGAAACGCTTCGCACAGCAGCTCGCTGTTg                                                             <  1:373217/100‑1 (MQ=255)
                                       cGGGATTTCGTCGCTGCGCTCTTTACGCAGATCGGCATCATCCGGCAAAGGTTGCCCGGTAAAGGCATGCAGAAACGCTTCGCACAGCAGCTCGCTGTTg                                                             <  1:4126978/100‑1 (MQ=255)
                                        gggATTTCGTCGCTGCGCTCTTTACGCAGATCGGCATCATCCGGCAAAGGTTGCCCGGTAAAGGCATGCAGAAACGCTTCGCACAGCAGCTCGCTGTTgg                                                            <  1:4358027/100‑1 (MQ=255)
                                         ggATTTCGTCGCTGCGCTCTTTACGCAGATCGGCATCATCCGGCAAAGGTTGCCCGGTAAAGGCATGCAGAAACGCTTCGCACAGCAGCTCGCTGTTGGt                                                           >  1:2224588/1‑100 (MQ=255)
                                         ggATTTCGTCGCTGCGCTCTTTACGCAGATCGGCATCATCCGGCAAAGGTTGCCCGGTAAAGGCATGCAGAAACGCTTCGCACAGCAGCTCGCTGTTGGt                                                           >  2:1887479/1‑100 (MQ=255)
                                             ttCGTCGCTGCGCTCTTTACGCAGATCGGCATCATCCGGCAAAGGTTGCCCGGTAAAGGCATGCAGAAACGCTTCGCACAGCAGCTCGCTGTTGGTAGCg                                                       <  2:2334628/100‑1 (MQ=255)
                                               cgtcgCTGCGCTCTTTACGCAGATCGGCATCATCCGGCAAAGGTTGCCCGGTACAGGCATGCAGAAACGCTTCGCACAGCAGCCCGCTGGTGGTCGCGt                                                      >  1:2579181/1‑99 (MQ=255)
                                               cgtcgCTGCGCTCTTTACGCAGATCGGCATCATCCGGCAAAGGTTGCCCGGTAAAGGCATGCAGAAACGCTTCGCACAGCAGCTCGCTGTTGGTAGCGTg                                                     <  2:2917802/100‑1 (MQ=255)
                                                gtcgCTGCGCTCTTTACGCAGATCGGCATCATCCGGCAAAGGTTGCCCGGTAAAGGCATGCAGAAACGCTTCGCACAGCAGCTCGCTGTTGGTAGCGTGa                                                    >  1:1852062/1‑100 (MQ=255)
                                                   gCTGCGCTCTTTACGCAGATCGGCATCATCCGGCAAAGGTTGCCCGGCAAAGGCATGCAGAAACGCTTCGCACAGCAGCTCGCTGTTGGTAGCGTGACGc                                                 <  2:4365916/100‑1 (MQ=255)
                                                    cTGCGCTCTTTACGCAGATCGGCATCATCCGGCAAAGGTTGCCCGGTAAAGGCATGCAGAAACGCTTCGCACAGCAGCTCGCTGTTGGTAGCGTGACGCa                                                <  2:402588/100‑1 (MQ=255)
                                                     tGCGCTCTTTACGCAGATCGGCATCATCCGGCAAAGGTTGCCCGGTAAAGGCATGCAGAAACGCTTCGCACAGCAGCTCGCTGTTGGTAGCGTGACGCAg                                               <  2:808152/100‑1 (MQ=255)
                                                      gcgcTCTTTACGCAGATCGGCATCATCCGGCAAAGGTTGCCCGGTAAAGGCATGCAGAAACGCTTCGCACAGCAGCTCGCTGTTGGTAGCGTGACGCAgg                                              <  1:473259/100‑1 (MQ=255)
                                                       cgcTCTTTACGCAGATCGGCATCATCCGGCAAAGGTTGCCCGGTAAAGGCATGCAGAAACGCTTCGCACAGCAGCTCGCTGTTGGTAGCGTGACGCAGgt                                             <  2:3850356/100‑1 (MQ=255)
                                                       cgcTCTTTACGCAGATCGGCATCATCCGGCAAAGGTTGCCCGGTAAAGGCATGCAGAAACGCTTCGCACAGCAGCTCGCTGTTGGTAGCGTGACGCAGgt                                             <  2:2665450/100‑1 (MQ=255)
                                                       cgcTCTTTACGCAGATCGACATCATCCGGCAACGGTTCCCCGGTAAAGGCATGCAGAAACGCTTCGCACAGCAGCTCGCTGTTGGTAGCGTGACGCAGgt                                             <  1:3985135/100‑1 (MQ=255)
                                                         ctctTTACGCAGATCGGCATCATCCGGCAAAGGTTGCCCGGCAAAGGCATGCAGAAACGCTTCGCACAGCAGCTCGCTGTTGGTAGCGTGACGCAGgttg                                           <  2:282822/100‑1 (MQ=255)
                                                          tctTTACGCAGATCGGCATCATCCGGCAAAGGTTGCCCGGTAAAGGAATGCAGAAACGCTTCGGACAGCAGCTCGCTGTTGGTAGCGTGACGCAGgttgt                                          >  2:1285667/1‑100 (MQ=255)
                                                           ctTTACGCAGATCGGCATCATCCGGCAAAGGTTGCCCGGTAAAGGCATGCAGAAACGCTTCGCACAGCAGCTCGCTGTTGGTAGCGTGACGCAGggtgtt                                         >  2:3631298/1‑100 (MQ=255)
                                                             ttACGCAGATCGGCATCATCCGGCAAAGGTTGCCCGGTAAAGGCATGCAGAGACGCTTCGCACAGCCGCTCGCTGTTGGTAGCGTGACGCAGGTTGTTCa                                       <  1:2873680/100‑1 (MQ=255)
                                                             ttACGCAGATCGGCATCATCCGGCAAAGGTTGCCCGGCAAAGGCATGCAGAAACGCTTCGCACAGCAGCTCGCTGTTGGTAGCGTGACGCAGGTTGTTCa                                       <  1:3254123/100‑1 (MQ=255)
                                                                cGCAGATCGGCATCATCCGGCAAAGGTTGCCCGGTAAAGGCATGCAGAAACGCTTCGCACAGCAGCTCGCTGTTGGTAGCGTGACGCAGGTTGTTCAcc                                     <  2:3885236/99‑1 (MQ=255)
                                                                cGCAGATCGGCATCATCCGGCAAAGGTTGCCCGGTAAAGGCATGCAGAAACGCTTCGCACAGCAGCTCGCTGTTGGTAGCGTGACGCAGGTTGTTCACCt                                    >  2:3159395/1‑100 (MQ=255)
                                                                cGCAGATCGGCATCATCCGGCAAAGGTTGCCCGGTAAAGGCATGCAGAAACGCTTCGCACAGCAGCTCGCTGTTGGTAGCGTGACGCAGGTTGTTCACCt                                    >  2:4316632/1‑100 (MQ=255)
                                                                cGCAGATCGGCATCATCCGGCAAAGGTTGCCCGGTAAAGGCATGCAGAAACGCTTCGCACAGCAGCTCGCTGTTGGTAGCGTGACGCAGGTTGTTCACCt                                    >  2:1958186/1‑100 (MQ=255)
                                                                 gCAGATCGGCATCATCCGGCAAAGGTTGCCCGGTAAAGGCATGCAGAAACGCTTCGCACAGCAGCTCGCTGTTGGTAGCGTGACGCAGGTTGTTCACCt                                    <  2:2496505/99‑1 (MQ=255)
                                                                       cGGCATCATCCGGCAAAGGTTGCCCGGTAAAGGCATGCAGAAACGCTTCGCACAGCAGCTCGCTGTTGGTAGCGTGACGCAGGTTGTTCGCCTGacgacg                             >  2:442167/1‑100 (MQ=255)
                                                                       cGGCATCATCCGGCAAAGGTTGCCCGGTAAAGGCATGCAGAAACGCTTCGCACAGCAGCTCGCTGTTGGTAGCGTGACGCAGGTTGTTCACCTGacgacg                             <  1:101007/100‑1 (MQ=255)
                                                                        ggCATCATCCGGCAATGGTTGCCCGGTAAAGGCATGCAGAAACGCTTCGCACAGCAGCTCGCTGTTGGTAGCGTGACGCAGGTTGTTCACCTGACGAcgc                            <  1:3970442/100‑1 (MQ=255)
                                                                        ggCATCATCCGGCAAAGGTTGCCCGGTAAAGGCATGCAGAAACGCTTCGCACAGCAGCTCGCTGTTGGTAGCGTGACGCAGGTTGTTCACCTGACGAcgc                            >  2:3611616/1‑100 (MQ=255)
                                                                        ggCATCATCCGGCAAAGGTTGCCCGGCAAAGGCATGCAGAAACGCTTCGCACAGCAGCTCGCTGTTGGTAGCGTGACGCAGGTTGTTCACCTGACGAcgc                            >  2:652220/1‑100 (MQ=255)
                                                                        ggCATCATCCGGCAAAGGTTGCCCGGCAAAGGCATGCAGAAACGCTTCGCACAGCAGCTCGCTGTTGGTAGCGTGACGCAGGTTGTTCACCTGACGAcgc                            >  2:1848863/1‑100 (MQ=255)
                                                                         gCATCATCCGGCAAGGGTTGCCCGGTAAAGGCATGCAGAAACGCTTCGCACAGCAGCTCGCTGTTGGTAGCGTGACGCAGGTTGTTCACCTGACGAcgc                            >  1:2130574/1‑99 (MQ=255)
                                                                         gCATCATCCGGCAAAGGTTGCCCGGTAAAGGCATGCAGAAACGCTTCGCACAGCAGCTCGCTGTTGGTAGCGTGACGCAGGTTGTTCACCTGACGAcgcg                           >  1:534335/1‑100 (MQ=255)
                                                                         gCATCATCCGGCAAAGGTTGCCCGGTAAAGGCATGCAGAAACGCTTCGCACAGCAGCTCGCTGTTGGTAGCGTGACGCAGGTTGTTCACCTGACGAcgcg                           <  2:1897552/100‑1 (MQ=255)
                                                                          catcatCCGGCAAAGGTTGCCCGGCAAAGGCATGCAGAAACGCTTCGCACAGCAGCTCGCTGTTGGTAGCGTGACGCAGGTTGTTCACCTGACGACgcgt                          >  1:2395510/1‑100 (MQ=255)
                                                                           atcatcCGGCAAAGGTTGCCCGGTAAAGGCATGCAGAAACGCTTCGCACAGCAGCTCGCTGTTGGTAGCGTGACGCAGGTTGTTCACCTGACGACgcgtg                         <  1:1285662/100‑1 (MQ=255)
                                                                           atcatcCGGCAAAGGTTGCCCGGTAAAGGCATGCAGAAACGCTTCGCACAGCAGCTCGCTGTTGGTAGCGTGACGCAGGTTGTTCACCTGACGACgcgtg                         <  2:1101895/100‑1 (MQ=255)
                                                                           atcatcCGGCAAAGGTTGCCCGGCAAAGGCATGCAGAAACGCTTCGCACAGCAGCTCGCTGTTGGTAGCGTGACGCAGGTTGTTCACCTGACGACgcgtg                         >  2:641455/1‑100 (MQ=255)
                                                                            tcatcCGGCAAAGGTTGCCCGGTAAAGGCATGCAGAAACGCTTCGCACAGCAGCTCGCTGTTGGTAGCGTGACGCAGGTTGTTCACCTGACGACgcgtgc                        <  2:303157/100‑1 (MQ=255)
                                                                             catcCGGCAAAGGTTGCCCGGTAAAGGCATGCAGAAACGCTTCGCACAGCAGCTCGCTGTTGGTAGCGTGACGCAGGTTGTTCGCCTGACGACgcgtgcg                       >  2:139711/1‑100 (MQ=255)
                                                                             catcCGGCAAAGGTTGCCCGGTAAAGGCATGCAGAAACGCTTCGCACAGCAGCTCGCTGTTGGTAGCGTGACGCAGGTTGTTCACCTGACGACgcgtgcg                       >  2:3715769/1‑100 (MQ=255)
                                                                              atcCGGCAAAGGTTGCCCGGTAAAGGCATGCAGAAACGCTTCGCACAGCAGCTCGCTGTTGGTAGCGTGACGCAGGTTGTTCACCTGACGACgcgtgcgt                      >  1:1427775/1‑100 (MQ=255)
                                                                              atcCGGCAAAGGTTGCCCGGCAAAGGCATGCAGAAACGCTTCGCACAGCAGCTCGCTGTTGGTAGCGTGACGCAGGTTGTTCACCTGACGACgcgtgcgt                      >  2:2645658/1‑100 (MQ=255)
                                                                               tcCGGCAAAGGTTGCCCGGTAAAGGCATGCAGAAACGCTTCGCACAGCAGCTCGCTGTTGGTAGCGTGACGCAGGTTGTTCACCTGACGACGCGTGCGtt                     >  2:3133237/1‑100 (MQ=255)
                                                                                ccGGCAAAGGTTGCCCGGTAAAGGCATGCAGAAACGCTTCGCACAGCAGCTCGCTGTTGGTAGCGTGACGCAGGTTGTTCACCTGACGACGCGTGCGTTc                    >  2:2223259/1‑100 (MQ=255)
                                                                                  ggCAAAGGTTGCCCGGTAAAGGCATGCAGAAACGCTTCGCACAGCAGCTCGCTGTTGGTAGCGTGACGCAGGTTGTTCGCCTGACGACGCGTGCGTTCAt                  >  2:2368481/1‑100 (MQ=255)
                                                                                    ccaaGGTTGCCCGGTCAAGGCATGCAGAAACGCTTCGCACAGCAGCTCGCTGTGGGTAGCGTGACGCAGGTTGTTCGCCTGACGACGCGTGCGTTCa                   >  1:1402709/3‑97 (MQ=255)
                                                                                       aGGTTGCCCGGTAAAGGCATGCAGAAACGCTTCGCACAGCAGCTCGCTGTTGGTAGCGTGACGCAGGTTGTTCACCTGACGACGCGTGCGTTCATCGGTg             <  1:3155216/100‑1 (MQ=255)
                                                                                        ggTTGCCCGGCAAAGGCATGCAGAAACGCTTCGCACAGCAGCTCGCTGTTGGTAGCGTGACGCAGGTTGTTCACCTGACGACGCGTGCGTTCATCGGTGa            >  2:3001627/1‑100 (MQ=255)
                                                                                        ggTTGCCCGGCAAAGGCATGCAGAAACGCTTCGCACAGCAGCTCGCTGTTGGTAGCGTGACGCAGGTTGTTCACCTGACGACGCGTGCGTTCATCGGTGa            >  1:1019306/1‑100 (MQ=255)
                                                                                         gTTGCCCGGTAAAGGCATGCAGAAACGCTTCGCACAGCAGCTCGCTGTTGGTAGCGTGACGCAGGTTGTTCACCTGACGACGCGTGCGTTCATCGGTGAg           <  2:4318744/100‑1 (MQ=255)
                                                                                         gTTGCCCGGTAAAGGCATGCAGAAACGCTTCGCACAGCAGCTCGCTGTTGGTAGCGTGACGCAGGTTGTTCACCTGACGACGCGTGCGTTCATCGGTGAg           <  2:4397311/100‑1 (MQ=255)
                                                                                          ttGCCCGGTAAAGGCATGCAGAAACGCTTCGCACAGCAGCTCGCTGTTGGTAGCGTGACGCAGGTTGTTCGCCTGACGACGCGTGCGTTCATCGGTGAgg          >  1:1587513/1‑100 (MQ=255)
                                                                                           tGCCCGGCAAAGGCATGCAGAAACGCTTCGCACAGCAGCTCGCTGTTGGTAGCGTGACGCAGGTTGTTCACCTGACGACGCGTGCGTTCATCGGTGAgg          >  1:2798762/1‑99 (MQ=255)
                                                                                            ncccGGTAAAGGCATGCAGAAACGCTTCGCACAGCAGCTCGCTGTTGGTAGCGTGACGCAGGTTGTTCGCCTGACGACGCGTGCGTTCATCGGTGAGGAt        >  1:3924153/2‑100 (MQ=255)
                                                                                             cccGGTAAAGGCATGCAGAAACGCTTCGCACAGCAGCTCGCTGTTGGTAGCGTGACGCAGGTTGTTCACCTGACGACGCGTGCGTTCATCGGTGAGGAtt       <  2:3216476/100‑1 (MQ=255)
                                                                                             cccGGTAAAGGCATGCAGAAACGCTTCGCACAGCAGCTCGCTGTTGGTAGCGTGACGCAGGTTGTTCACCTGACGACGCGTGCGTTCATCGGTGAGGAtt       >  2:1715890/1‑100 (MQ=255)
                                                                                              ccGGTAAAGGCATGCAGAAACGCTTCGCACAGCAGCTCGCTGTTGGTAGCGTGACGCAGGTTGTTCACCTGACGACGCGTGCGTTCATCGGTGAGGAttt      >  1:2976323/1‑100 (MQ=255)
                                                                                              ccGGTAAAGGCATGCAGAAACGCTTCGCACAGCAGCTCGCGGGTGGTAGCGTGACGCAGGCTGTTCCCCTGACGACGCGTGCGTTCATCGGTGAgg          >  1:1336342/1‑96 (MQ=255)
                                                                                                 gTAAAGGCATGCAGAAACGCTTCGCACAGCAGCTCGCTGTTGGTAGCGTGACGCAGGTTGTTCACCTGACGACGCGTGCGTTCATCGGTGAGGATTTTTa   <  2:1399829/100‑1 (MQ=255)
                                                                                                  tAAAGGCATGCAGAAACGCTTCGCACAGCAGCTCGGTGTTGGTAGCGTGACGCAGGTTGTTTACCTGACGACGCGTGCGTTCATCGGTGAGGATTTTTaa  <  1:3566327/100‑1 (MQ=255)
                                                                                                  |                                                                                                   
GTTAATCCCCATCTCACGCATGATCTCTTTTGCCGCTTCCGGGATTTCGTCGCTGCGCTCTTTACGCAGATCGGCATCATCCGGCAAAGGTTGCCCGGTAAAGGCATGCAGAAACGCTTCGCACAGCAGCTCGCTGTTGGTAGCGTGACGCAGGTTGTTCACCTGACGACGCGTGCGTTCATCGGTGAGGATTTTTAA  >  NC_000913/4128099‑4128296

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A