Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A4 F191 I0 R1 105 142.7 6924153 96.8% 6702580 99.9

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 4,128,197 T→C 59.3% T67A (ACC→GCC)  metJ ← transcriptional repressor, S‑adenosylmethionine‑binding

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,128,1970TC59.3% 85.8 / 131.4 118T67A (ACC→GCC) metJtranscriptional repressor, S‑adenosylmethionine‑binding
Reads supporting (aligned to +/- strand):  ref base T (21/27);  new base C (40/30);  total (61/57)
Fisher's exact test for biased strand distribution p-value = 1.90e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.88e-01

Only 100 of 118 total aligned reads displayed.

AATCCCCATCTCACGCATGATCTCTTTTGCCGCTTCCGGGATTTCGTCGCTGCGCTCTTTACGCAGATCGGCATCATCCGGCAAAGGTTGCCCGGTAAAGGCATGCAGAAACGCTTCGCACAGCAGCTCGCTGTTGGTAGCGTGACGCAGGTTGTTCACCTGACGACGCGTGCGTTCATCGGTGAGGATTTTTA  >  NC_000913/4128102‑4128295
                                                                                               |                                                                                                  
aaTCCCCATCTCACGCATGATCTCTTTTGCCGCTTCCGGGATTTCGTCGCTGCGCTCTTTACGCAGATCGGCATCATCCGGCAAAGGTTGCCCGGCAAAg                                                                                                >  1:1103139/1‑100 (MQ=255)
  tCCCCATCTCACGCATGATCTCTTTTGCCGCTTCCGGGATTTCGTCGCTGCGCTCTTTACGCAGATCGGCATCATCCGGCAAAGGTTGCCCGGTAAAGGc                                                                                              >  1:3193651/1‑100 (MQ=255)
  tCCCCATCTCACGCATGATCTCTTTTGCCGCTTCCGGGATTTCGTCGCTGCGCTCTTTACGCAGATCGGCATCATCCGGCAAAGGTTGCCCGGCAAAGGc                                                                                              >  1:1461650/1‑100 (MQ=255)
   ccccATCTCACGCATGATCTCTTTTGCCGCTTCCGGGATTTCGTCGCTGCGCTCTTTACGCAGATCGGCATCATCCGGCAAAGGTTGCCCGGTAAAGGCa                                                                                             <  2:1253938/100‑1 (MQ=255)
   ccccATCTCACGCATGATCTCTTTTGCCGCTTCCGGGATTTCGTCGCTGCGCTCTTTACGCAGATCGGCATCATCCGGCAAAGGTTGCCCGGCAAAGGCa                                                                                             >  2:1487449/1‑100 (MQ=255)
     ccATCTCACGCATGATCTCTTTTGCCGCTTCCGGGATTTCGTCGCTGCGCTCTTTACGCAGATCGGCATCATCCGGCAAAGGTTGCCCGGCAAAGGCATg                                                                                           <  1:2632989/100‑1 (MQ=255)
       aTCTCACGCATGATCTCTTTTGCCGCTTCCGGGATTTCGTCGCTGCGCTCTTTACGCAGATCGGCATCATCCGGCAAAGGTTGCCCGGCAAAGGCATGCa                                                                                         <  2:783114/100‑1 (MQ=255)
         ctcACGCATGATCTCTTTTGCCGCTTCCGGGATTTCGTCGCTGCGCTCTTTACGCAGATCGGCATCATCCGGCAAAGGTTGCCCGGTAAAGGCATGCAGa                                                                                       >  2:2492475/1‑100 (MQ=255)
         ctcACGCATGATCTCTTTTGCCGCTTCCGGGATTTCGTCGCTGCGCTCTTTACGCAGATCGGCATCATCCGGCAAAGGTTGCCCGGTAAAGGCATGCAGa                                                                                       >  1:913833/1‑100 (MQ=255)
          tcACGCATGATCTCTTTTGCCGCTTCCGGGATTTCGTCGCTGCGCTCTTTACGCAGATCGGCATCATCCGGCAAAGGTTGCCCGGTAAAGGCATGCAGaa                                                                                      <  1:379517/100‑1 (MQ=255)
            aCGCATGATCTCTTTTGCCGCTTCCGGGATTTCGTCGCTGCGCTCTTTACGCAGATCGGCATCATCCGGCAAAGGTTGCCCGGCAAAGGCATGCAGAAAc                                                                                    >  1:2821254/1‑100 (MQ=255)
             cGCATGATCTCTTTTGCCGCTTCCGGGATTTCGTCGCTGCGCTCTTTACGCAGATCGGCATCATCCGGCAAAGGTTGCCCGGCAAAGGCATGCAGAAACg                                                                                   >  1:101325/1‑100 (MQ=255)
               cATGATCTCTTTTGCCGCTTCCGGGATTTCGTCGCTGCGCTCTTTACGCAGATCGGCATCATCCGGCAAAGGTTGCCCGGTAAAGGCATGCAGAAACGCt                                                                                 >  1:1417811/1‑100 (MQ=255)
               cATGATCTCTTTTGCCGCTTCCGGGATTTCGTCGCTGCGCTCTTTACGCAGATCGGCATCATCCGGCAAAGGTTGCCCGGCAAAGGCATGCAGAAACGCt                                                                                 >  2:2066235/1‑100 (MQ=255)
                aTGGTCTCTTTTGCCGCTGCCGGGATTTCGTCGCTGCGGTCTTTACGCAGATCGGCATCATCCGGCAAAGGTTGCCCGGTAAAGGCATGCAGAAACGCtg                                                                                <  1:3070609/100‑2 (MQ=255)
                   aTCTCTTTTGCCGCTTCCGGGATTTCGTCGCTGCGCTCTTTACGCAGATCGGCATCATCCGGCAAAGGTTGCCCGGTAAAGGCATGCAGAAACGCTTCGc                                                                             >  1:3179494/1‑100 (MQ=255)
                   aTCTCTTTTGCCGCTTCCGGGATTTCGTCGCTGCGCTCTTTACGCAGATCGGCATCATCCGGCAAAGGTTGCCCGGCAAAGGCATGCAGAAACGCTTCGc                                                                             <  2:2121294/100‑1 (MQ=255)
                   aTCTCTTTTGCCGCTTCCGGGATTTCGTCGCTGCGCTCTTTACGCAGATCGGCATCATCCGGCAAAGGTTGCCCGGCAAAGGCATGCAGAAACGCTTCGc                                                                             <  1:2190589/100‑1 (MQ=255)
                   aTCTCTTTTGCCGCTTCCGGGATTTCGTCGCTGCGCTCTTTACGCAGATCGGCATCATCCGGCAAAGGTTGCCCGGCAAAGGCATGCAGAAACGCTTCGc                                                                             <  1:2752690/100‑1 (MQ=255)
                     ctctTTTGCCGCTTCCGGGATTTCGTCGCTGCGCTCTTTACGCAGATCGGCATCATCCGGCAAAGGTTGCCCGGCAAAGGCATGCAGAAACGCTTCGcac                                                                           >  1:1143319/1‑100 (MQ=255)
                       ctTTTGCCGCTTCCGGGATTTCGTCGCTGCGCTCTTTACGCAGATCGGCATCATCCGGCAAAGGTTGCCCGGTAAAGGCATGCAGAAACGCTTCGCAcag                                                                         >  1:3283484/1‑100 (MQ=255)
                        ttttGCCGCTTCCGGGATTTCGTCGCTGCGCTCTTTACGCAGATCGGCATCATCCGGCAAAGGTTGCCCGGTAAAGGCATGCAGAAACGCTTCGCAcagc                                                                        <  2:3382455/100‑1 (MQ=255)
                        ttttGCCGCTTCCGGGATTTCGTCGCTGCGCTCTTTACGCAGATCGGCATCATCCGGCAAAGGTTGCCCGGCAAAGGCATGCAGAAACGCTTCGCAcagc                                                                        <  2:1556207/100‑1 (MQ=255)
                        ttttGCCGCTTCCGGGATTTCGTCGCTGCGCTCTTTACGCAGATCGGCATCATCCGGCAAAGGTTGCCCGGCAAAGGCATGCAGAAACGCTTCGCAcagc                                                                        <  1:2701017/100‑1 (MQ=255)
                         tttGCCGCTTCCGGGATTTCGTCGCTGCGCTCTTTACGCAGATCGGCATCATCCGGCAAAGGTTGCCCGGTAAAGGCATGCAGAAACGCTTCGCAcagca                                                                       <  1:2586958/100‑1 (MQ=255)
                            gCCGCTTCCGGGATTTCGTCGCTGCGCTCTTTACGCAGATCGGCATCATCCGGCAAAGGTTGCCCGGTAAAGGCATGCAGAAACGCTTCGCAcagcag                                                                      >  2:156611/1‑98 (MQ=255)
                            gCCGCTTCCGGGATTTCGTCGCTGCGCTCTTTACGCAGATCGGCATCATCCGGCAAAGGTTGCCCGGTAAAGGCATGCAGAAACGCTTCGCACAGCAGCt                                                                    >  1:559382/1‑100 (MQ=255)
                            gCCGCTTCCGGGATTTCGTCGCTGCGCTCTTTACGCAGATCGGCATCATCCGGCAAAGGTTGCCCGGCAAAGGCATGCAGAAACGCTTCGCACAGCAGCt                                                                    <  1:1504347/100‑1 (MQ=255)
                            gCCGCTTCCGGGATTTCGTCGCTGCGCTCTTTACGCAGATCGGCATCATCCGGCAAAGGTTGCCCGGCAAAGGCATGCAGAAACGCTTCGCACAGCAGCt                                                                    >  1:3190999/1‑100 (MQ=255)
                             ccGCTTCCGGGATTTCGTCGCTGCGCTCTTTACGCAGATCGGCATCATCCGGCAAAGGTTGCCCGGCAAAGGCATGCAGAAACGCTTCGCACAGCAGCTc                                                                   >  1:595415/1‑100 (MQ=255)
                             ccGCTTCCGGGATTTCGTCGCTGCGCTCTTTACGCAGATCGGCATCATCCGGCAAAGGTTGCCCGGCAAAGGCATGCAGAAACGCTTCGCACAGCAGCTc                                                                   >  2:2844199/1‑100 (MQ=255)
                              cGCTTCCGGGATTTCGTCGCTGCGCTCTTTACGCAGATCGGCATCATCCGGCAAAGGTTGCCCGGTAAAGGCATGCAGAAACGCTTCGCACAGCAGCTCg                                                                  <  2:2304876/100‑1 (MQ=255)
                              cGCTTCCGGGATTTCGTCGCTGCGCTCTTTACGCAGATCGGCATCATCCGGCAAAGGTTGCCCGGTAAAGGCATGCAGAAACGCTTCGCACAGCAGCTCg                                                                  <  2:3339710/100‑1 (MQ=255)
                               tcTTCCGGGATTTCGTCGCTGCGCTCTTTACGCAGATCGGCATCATCCGGCAAAGGTTGCCCGGCAAAGGCATGCAGAAACGCTTCGCACAGCAGCTCGc                                                                 <  1:3294409/99‑1 (MQ=255)
                                cTTCCGGGATTTCGTCGCTGCGCTCTTTACGCAGATCGGCATCATCCGGCAAAGGTTGCCCGGCAAAGGCATGCAGAAACGCTTCGCACAGCAGCTCGca                                                                >  2:3294433/1‑99 (MQ=255)
                                  tCCGGGATTTCGTCGCTGCGCTCTTTACGCAGATCGGCATCATCCGGCAAAGGTTGCCCGGTAAAGGCATGCAGAAACGCTTCGCACAGCAGCTCGCTGt                                                              >  1:1707704/1‑100 (MQ=255)
                                  tCCGGGATTTCGTCGCTGCGCTCTTTACGCAGATCGGCATCATCCGGCAAAGGTTGCCCGGTAAAGGCATGCAGAAACGCTTCGCACAGCAGCTCGCTGt                                                              >  1:2940305/1‑100 (MQ=255)
                                  tCCGGGATTTCGTCGCTGCGCTCTTTACGCAGATCGGCATCATCCGGCAAAGGTTGCCCGGCAAAGGCATGCAGAAACGCTTCGCACAGCAGCTCGCTGt                                                              <  1:3424217/100‑1 (MQ=255)
                                   ccGGGATTTCGTCGCTGCGCTCTTTACGCAGATCGGCATCATCCGGCAAAGGTTGCCCGGCAAAGGCATGCAGAAACGCTTCGCACAGCAGCTCGCTGtt                                                             <  1:99373/100‑1 (MQ=255)
                                   ccGGGATTTCGTCGCTGCGCTCTTTACGCAGATCGGCATCATCCGGCAAAGGTTGCCCGGCAAAGGCATGCAGAAACGCTTCGCACAGCAGCTCGCTGtt                                                             <  1:3372767/100‑1 (MQ=255)
                                    cGGGATTTCGTCGCTGCGCTCTTTACGCAGATCGGCATCATCCGGCAAAGGTTGCCCGGCAAAGGCATGCAGAAACGCTTCGCACAGCAGCTCGCTGTTg                                                            >  1:2522213/1‑100 (MQ=255)
                                    cGGGATTTCGTCGCTGCGCTCTTTACGCAGATCGGCATCATCCGGCAAAGGTTGCCCGGCAAAGGCATGCAGAAACGCTTCGCACAGCAGCTCGCTGTTg                                                            >  1:2849358/1‑100 (MQ=255)
                                     gggATTTCGTCGCTGCGCTCTTTACGCAGATCGGCATCATCCGGCAAAGGTTGCCCGGTAAAGGCATGCAGAAACGCTTCGCACAGCAGCTCGCTGTTgg                                                           <  2:694713/100‑1 (MQ=255)
                                         tttCGTCGCTGCGCTCTTTACGCAGATCGGCATCATCCGGCAAAGGTTGCCCGGCAAAGGCATGCAGAAACGCTTCGCACAGCAGCTCGCTGTTGGTAGc                                                       <  2:2450418/100‑1 (MQ=255)
                                          ttCGTCGCTGCGCTCTTTACGCAGATCGGCATCATCCGGCAAAGGTTGCCCGGTAAAGGCATGCAGAAACGCTTCGCACAGCAGCTCGCTGTTGGTAGCg                                                      <  1:247543/100‑1 (MQ=255)
                                          ttCGTCGCTGCGCTCTTTACGCAGATCGGCATCATCCGGCAAAGGTTGCCCGGCAAAGGCATGCAGAAACGCTTCGCACAGCAGCTCGCTGTTGGTAGCg                                                      <  1:805229/100‑1 (MQ=255)
                                          ttCGTCGCTGCGCTCTTTACGCAGATCGGCATCATCCGGCAAAGGTTGCCCGGCAAAGGCATGCAGAAACGCTTCGCACAGCAGCTCGCTGTTGGTAGCg                                                      >  2:228046/1‑100 (MQ=255)
                                          ttCGTCGCTGCGCTCTTTACGCAGATCGGCATCATCCGGCAAAGGTTGCCCGGCAAAGGCATGCAGAAACGCTTCGCACAGCAGCTCGCTGTTGGTAGCg                                                      >  1:781888/1‑100 (MQ=255)
                                           tcgtcgCTGCGCTCTTTACGCAGATCGGCATCATCCGGCAAAGGTTGCCCGGTAAAGGCATGCAGAAACGCTTCGCACAGCAGCTCGCTGTTGGTAGCGt                                                     <  2:1890107/100‑1 (MQ=255)
                                           ncgtcgCTGCGCTCTTTACGCAGATCGGCATCATCCGGCAAAGGTTGCCCGGCAAAGGCATGCAGAAACGCTTCGCACAGCAGCTCGCTGTTGGTAGCGt                                                     >  1:994110/2‑100 (MQ=255)
                                            cgtcgCTGCGCTCTTTACGCAGATCGGCATCATCCGGCAAAGGTTGCCCGGCAAAGGCATGCAGAAACGCTTCGCACAGCAGCTCGCTGTTGGTAGCGTg                                                    <  1:1687704/100‑1 (MQ=255)
                                               cgCTGCGCTCTTTACGCAGATCGGCATCATCCGGCAAAGGTTGCCCGGCAAAGGCATGCAGAAACGCTTCGCACAGCAGCTCGCTGTTGGTAGCGTGACg                                                 >  2:731209/1‑100 (MQ=255)
                                                gCTGCGCTCTTTACGCAGATCGGCATCATCCGGCAAAGGTTGCCCGGTAAAGGCATGCAGAAACGCTTCGCACAGCAGCTCGCTGTTGGTAGCGTGACGc                                                <  2:3193675/100‑1 (MQ=255)
                                                 cTGCGCTCTTTACGCAGATCGGCCCCATCCGGCAACGGTTGCCCGGCACAGGCATGCAGAAACGCCTCGCACAGCAGCTCGCTGTTGGTAGCGTGACGc                                                >  1:3177208/1‑99 (MQ=255)
                                                 cTGCGCTCTTTACGCAGATCGGCATCATCCGGCAAAGGTTGCCCGGTAAAGGCATGCAGAAACGCTTCGCACAGCAGCTCGCTGTTGGTAGCGTGACGCa                                               >  2:761359/1‑100 (MQ=255)
                                                   gcgcTCTTTACGCAGATCGGCATCATCCGGCAAAGGTTGCCCGGCAAAGGCATGCAGAAACGCTTCGCACAGCAGCTCGCTGTTGGTAGCGTGACGCAgg                                             <  2:3171775/100‑1 (MQ=255)
                                                     gcTCTTTACGCAGATCGGCATCATCCGGCAAAGGTTGCCCGGCAAAGGCATGCAGAAACGCTTCGCACAGCAGCTCGCTGTTGGTAGCGTGACGCAGgtt                                           >  1:1542612/1‑100 (MQ=255)
                                                       tctTTACGCAGATCGGCATCATCCGGCAAAGGTTGCCCGGTAAAGGCATGCAGAAACGCTTCGCACAGCAGCTCGCTGTTGGTAGCGTGACGCAGgttgt                                         <  1:2528333/100‑1 (MQ=255)
                                                        ctTTACGCAGATCGGCATCATCCGGCAAAGGTTGCCCGGCAAAGGCATGCAGAAACGCTTCGCACAGCAGCTCGCTGTTGGTAGCGTGACGCAGgttgtt                                        >  1:2730356/1‑100 (MQ=255)
                                                          ttACGCAGATCGGCATCATCCGGCAAAGGTTGCCCGGTAAAGGCATGCAGAAACGCTTCGCACAGCAGCTCGCTGTTGGTAGCGTGACGCAGGTTGTTCa                                      <  1:1208059/100‑1 (MQ=255)
                                                          ttACGCAGATCGGCATCATCCGGCAAAGGTTGCCCGGCAAAGGCATGCAGAAACGCTTCGCACAGCAGCTCGCTGTTGGTAGCGTGACGCAGGTTGTTCa                                      <  2:1534963/100‑1 (MQ=255)
                                                           tACGCAGATCGGCATCATCCGGCAAAGGTTGCCCGGTAAAGGCATGCAGAAACGCTTCGCACAGCAGCTCGCTGTTGGTAGCGTGACGCAGGTTGTTCAc                                     <  2:2597567/100‑1 (MQ=255)
                                                            aCGCAGATCGGCATCATCCGGCAAAGGTTGCCCGGTAAAGGCATGCAGAAACGCTTCGCACAGCAGCTCGCTGTTGGTAGCGTGACGCAGGTTGTTCAcc                                    <  2:236546/100‑1 (MQ=255)
                                                            aCGCAGATCGGCATCATCCGGCAAAGGTTGCCCGGCAAAGGCATGCAGAAACGCTTCGCACAGCAGCTCGCTGTTGGTAGCGTGACGCAGGTTGTTCAcc                                    <  2:2522232/100‑1 (MQ=255)
                                                            aCGCAGATCGGCATCATCCGGCAAAGGTTGCCCGGCAAAGGCATGCAGAAACGCTTCGCACAGCAGCTCGCTGTTGGTAGCGTGACGCAGGTTGTTCAcc                                    >  1:2890168/1‑100 (MQ=255)
                                                            aCGCAGATCGGCATCATCCGGCAAAGGTTGCCCGGCAAAGGCATGCAGAAACGCTTCGCACAGCAGCTCGCTGTTGGTAGCGTGACGCAGGATGTTCAcc                                    <  1:1639819/100‑1 (MQ=255)
                                                             cGCAGATCGGCATCATCCGGCAAAGGTTGCCCGGCAAAGGCATGCAGAAACGCTTCGCACAGCAGCTCGCTGTTGGTAGCGTGACGCAGGTTGTTCACCt                                   >  2:629155/1‑100 (MQ=255)
                                                              gCAGATCGGCATCATCCGGCAAAGGTTGCCCGGCAAAGGCATGCAGAAACGCTTCGCACAGCAGCTCGCTGTTGGTAGCGTGACGCAGGTTGTTCACCTg                                  <  2:1802337/100‑1 (MQ=255)
                                                               cAGATCGGCATCATCCGGCAAAGGTTGCCCGGCAAAGGCATGCAGAAACGCTTCGCACAGCAGCTCGCTGTTGGTAGCGTGACGCAGGTTGTTCACCTga                                 >  2:913704/1‑100 (MQ=255)
                                                                aGATCGGCATCATCCGGCAAAGGTTGCCCGGTAAAGGCATGCAGAAACGCTTCGCACAGCAGCTCGCTGTTGGTAGCGTGACGCAGGTTGTTCACCTgac                                <  2:2271090/100‑1 (MQ=255)
                                                                 gATCGGCATCATCCGGCAAAGGTTGCCCGGTAAAGGCATGCAGAAACGCTTCGCACAGCAGCTCGCTGTTGGTAGCGTGACGCAGGTTGTTCACCTgacg                               >  2:671960/1‑100 (MQ=255)
                                                                  aTCGGCATCATCCGGCAAAGGTTGCCCGGCAAAGGCATGCAGAAACGCTTCGCACAGCAGCTCGCTGTTGGTAGCGTGACGCAGGTTGTTCACCTgacga                              >  2:3164027/1‑100 (MQ=255)
                                                                   tCGGCATCATCCGGCAAAGGTTGCCCGGCAAAGGCATGCAGAAACGCTTCGCACAGCAGCTCGCTGTTGGTAGCGTGACGCAGGTTGTTCACCTgacgac                             >  1:2040488/1‑100 (MQ=255)
                                                                    cGGCATCATCCGGCAAAGGTTGCCCGGTAAAGGCATGCAGAAACGCTTCGCACAGCAGCTCGCTGTTGGTAGCGTGACGCAGGTTGTTCACCTGacgacg                            <  2:1152014/100‑1 (MQ=255)
                                                                     ggCATCATCCGGCAAAGGTTGCCCGGTAAAGGCATGCAGAAACGCTTCGCACAGCAGCTCGCTGTTGGTAGCGTGACGCAGGTTGTTCACCTGACGAcgc                           <  2:2730136/100‑1 (MQ=255)
                                                                      gCATCATCCGGCAAAGGTTGCCCGGTAAAGGCATGCTGAAACGCTTCGCACAGCAGCTCGCTGTTGGTAGCGTGACGCCGGTTGTTCACCTGacgacgag                          >  1:1096554/1‑98 (MQ=255)
                                                                      gCATCATCCGGCAAAGGTTGCCCGGTAAAGGCATGCAGAAACGCTTCGCACAGCAGCTCGCTGTTGGTAGCGTGACGCAGGTTGTTCACCTGACGAcgcg                          >  1:1178559/1‑100 (MQ=255)
                                                                      gCATCATCCGGCAAAGGTTGCCCGGTAAAGGCATGCAGAAACGCTTCGCACAGCAGCTCGCTGTTGGTAGCGTGACACAGGTTGTTCACCTGACGAcgcg                          >  1:2046306/1‑100 (MQ=255)
                                                                      gCATCATCCGGCAAAGGTTGCCCGGCAAAGGCATGCAGAAACGCTTCGCACAGCAGCTCGCTGTTGGTAGCGTGACGCAGGTTGTTCACCTGacgacg                            >  1:3034405/1‑98 (MQ=255)
                                                                      gCATCATCCGGCAAAGGTTGCCCGGCAAAGGCATGCAGAAACGCTTCGCACAGCAGCTCGCTGTTGGTAGCGTGACGCAGGTTGTTCACCTGACGAcgcg                          <  2:1897446/100‑1 (MQ=255)
                                                                        atcatcCGGCAAAGGTTGCCCGGCAAAGGCATGCAGAAACGCTTCGCACAGCAGCTCGCTGTTGGTAGCGTGACGCAGGTTGTTCACCTGACGACgcgtg                        <  1:3398917/100‑1 (MQ=255)
                                                                          catcCGGCAAAGGTTGCCCGGCAAAGGCATGCAGAAACGCTTCGCACAGCAGCTCGCTGTTGGTAGCGTGACGCAGGTTGTTCACCTGACGACgcgtgcg                      <  1:816867/100‑1 (MQ=255)
                                                                          catcCGGCAAAAGTTGCCCGGTAAAGGCATGCAGAAACGCTTCGCACAGCAGCTCGCTGTTGGTAGTGTGACGCAGGTTGTTCACCTGACGACgcgtgc                       <  2:3283508/99‑1 (MQ=255)
                                                                           atcCGGCAAAGGTTGCCCGGTAAAGGCATGCAGAAACGCTTCGCACAGCAGCTCGCTGTTGGTAGCGTGACGCAGGTTGTTCACCTGACGACgcgtgcgt                     >  2:2514699/1‑100 (MQ=255)
                                                                           atcCGGCAAAGGTTGCCCGGCAAAGGCATGCAGAAACGCTTCGCACAGCAGCTCGCTGTTGGTAGCGTGACGCAGGTTGTTCACCTGACGACgcgtgcgt                     >  1:777973/1‑100 (MQ=255)
                                                                           atcCGGCAAAGGTTGCCCGGCAAAGGCATGCAGAAACGCTTCGCACAGCAGCTCGCTGTTGGTAGCGTGACGCAGGTTGTTCACCTGACGACgcgtgcgt                     >  1:610082/1‑100 (MQ=255)
                                                                              cGGCAAAGGTTGCCCGGCAAAGGCATGCAGAAACGCTTCGCACAGCAGCTCGCTGTTGGTAGCGTGACGCAGGTTGTTCACCTGACGACGCGTGCGTTCa                  >  1:1899625/1‑100 (MQ=255)
                                                                               ggCAAAGGTTGCCCGGCAAAGGCATGCAGAAACGCTTCGCACAGCAGCTCGCTGTTGGTAGCGTGACGCAGGTTGTTCACCTGACGACGCGTGCGTTCAt                 >  2:96098/1‑100 (MQ=255)
                                                                               ggCAAAGGTTGCCCGGCAAAGGCATGCAGAAACGCTTCGCACAGCAGCTCGCTGTTGGTAGCGTGACGCAGGTTGTTCACCTGACGACGCGTGCGTTCAt                 >  1:1381039/1‑100 (MQ=255)
                                                                               ggCAAAGGTTGCCCGGCAAAGGCATGCAGAAACGCTTCGCACAGCAGCTCGCTGTTGGTAGCGTGACGCAGGTTGTTCACCTGACGACGCGTGCGTTCAt                 >  2:3192642/1‑100 (MQ=255)
                                                                                gatctggTTGCCCGGTAAAGGCATGCAGAAACGCTTCGCACAGCAGCTCGCTGTTGGTAGCGTGACGCAGGTTGTTCACCTGACGACGCGTGCGTTCATc                <  1:2392380/95‑1 (MQ=255)
                                                                                gCAAAGGTTGCCCGGTAAAGGCATGCAGAAACGCTTCGCACAGCAGCTCGCTGTTGGTAGCGTGACGCAGGTTGTTCACCTGACGACGCGTGCGTTCATc                <  2:913840/100‑1 (MQ=255)
                                                                                 cAAAGGTTGCCCGGTAAAGGCATGCAGAAACGCTTCGCACAGCAGCTCGCTGTTGGTAGCGTGACGCAGGTTGTTCACCTGACGACGCGTGCGTTCATCg               >  1:591586/1‑100 (MQ=255)
                                                                                     ggTTGCCCGGTAAAGGCATGCAGAAACGCTTCGCACAGCAGCTCGCTGTTGGTAGCGTGACGCAGGTTGTTCACCTGACGACGCGTGCGTTCATcagatc           >  2:2392399/1‑95 (MQ=255)
                                                                                     ggTTGCCCGGCAAAGGCATGCAGAAACGCTTCGCACAGCAGCTCGCTGTTGGTAGCGTGACGCAGGTTGTTCACCTGACGACGCGTGCGTTCATCGGTGa           <  2:3016698/100‑1 (MQ=255)
                                                                                      gTTGCCCGGCAAAGGCATGCAGAAACGCTTCGCACAGCAGCTCGCTGTTGGTAGCGTGACGCAGGTTGTTCACCTGACGACGCGTGCGTTCATCGGTGAg          <  2:2821277/100‑1 (MQ=255)
                                                                                         gCCCGGTAAAGGCATGCAGAAACGCTTCGCACAGCAGCTCGCTGTTGGTAGCGTGACGCAGGTTGTTCACCTGACGACGCGTGCGTTAATCGGTGAGGa        >  1:3205984/1‑99 (MQ=255)
                                                                                          cccGGTAAAGGCATGCAGAAACGCTTCGCACAGCAGCTCGCTGTTGGTAGCGTGACGCAGGTTGTTCACCTGACGACGCGTGCGTTCATCGGTGAGGAtt      <  1:2331074/100‑1 (MQ=255)
                                                                                           ccGGCAAAGGCATGCAGAAACGCTTCGCACAGCAGCTCGCTGTTGGTAGCGTGACGCAGGTTGTTCACCTGACGACGCGTGCGTTCATCGGTGAGGAttt     >  2:431463/1‑100 (MQ=255)
                                                                                              gTAAAGGCATGCAGAAACGCTTCGCACAGCAGCTCGCTGTTGGTAGCGTGACGCAGGTTGTTCACCTGACGACGCGTGCGTTCATCGGTGAGGATTTTTa  <  2:1727698/100‑1 (MQ=255)
                                                                                               |                                                                                                  
AATCCCCATCTCACGCATGATCTCTTTTGCCGCTTCCGGGATTTCGTCGCTGCGCTCTTTACGCAGATCGGCATCATCCGGCAAAGGTTGCCCGGTAAAGGCATGCAGAAACGCTTCGCACAGCAGCTCGCTGTTGGTAGCGTGACGCAGGTTGTTCACCTGACGACGCGTGCGTTCATCGGTGAGGATTTTTA  >  NC_000913/4128102‑4128295

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A