Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A4 F191 I1 R1 67 105.9 5535243 92.7% 5131170 99.9

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 4,128,197 T→C T67A (ACC→GCC)  metJ ← transcriptional repressor, S‑adenosylmethionine‑binding

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,128,1970TC95.8% 401.6 / 4.1 118T67A (ACC→GCC) metJtranscriptional repressor, S‑adenosylmethionine‑binding
Reads supporting (aligned to +/- strand):  ref base T (1/4);  new base C (56/57);  total (57/61)
Fisher's exact test for biased strand distribution p-value = 3.66e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.81e-01

Only 100 of 118 total aligned reads displayed.

TAATCCCCATCTCACGCATGATCTCTTTTGCCGCTTCCGGGATTTCGTCGCTGCGCTCTTTACGCAGATCGGCATCATCCGGCAAAGGTTGCCCGGTAAAGGCATGCAGAAACGCTTCGCACAGCAGCTCGCTGTTGGTAGCGTGACGCAGGTTGTTCACCTGACGACGCGTGCGTTCATCGGTGAGGATTT  >  NC_000913/4128101‑4128292
                                                                                                |                                                                                               
tAATCCCCATCTCACGCATGATCTCTTTTGCCGCTTCCGGGATTTCGTCGCTGCGCTCTTTACGCAGATCGGCATCATCCGGCAAAGGTTGCCCGGCaaa                                                                                              >  2:1161573/1‑100 (MQ=255)
      ccATCTCACGCATGATCTCTTTTGCCGCTTCCGGGATTTCGTCGCTGCGCTCTTTACGCAGATCGGCATCATCCGGCAAAGGTTGCCCGGCAAAGGCATg                                                                                        <  1:399257/100‑1 (MQ=255)
      ccATCTCACGCATGATCTCTTTTGCCGCTTCCGGGATTTCGTCGCTGCGCTCTTTACGCAGATCGGCATCATCCGGCAAAGGTTGCCCGGCAAAGGCATg                                                                                        <  2:1590192/100‑1 (MQ=255)
       cATCTCACGCATGATCTCTTTTGCCGCTTCCGGGATTTCGTCGCTGCGCTCTTTACGCAGATCGGCATCATCCGGCAAAGGTTGCCCGGCAAAGGCATGc                                                                                       <  1:465531/100‑1 (MQ=255)
       cATCTCACGCATGATCTCTTTTGCCGCTTCCGGGATTTCGTCGCTGCGCTCTTTACGCAGATCGGCATCATCCGGCAAAGGTTGCCCGGCAAAGGCATGc                                                                                       <  1:1161580/100‑1 (MQ=255)
        aTCTCACGCATGATCTCTTTTGCCGCTTCCGGGATTTCGTCGCTGCGCTCTTTACGCAGATCGGCATCATCCGGCAAAGGTTGCCCGGCAAAGGCATGCa                                                                                      >  1:634125/1‑100 (MQ=255)
         tctcACGCATGATCTCTTTTGCCGCTTCCGGGATTTCGTCGCTGCGCTCTTTACGCAGATCGGCATCATCCGGCAAAGGTTGCCCGGCAACGGCATGCAg                                                                                     >  2:1274010/1‑100 (MQ=255)
         tctcACGCATGATCTCTTTTGCCGCTTCCGGGATTTCGTCGCTGCGCTCTTTACGCAGATCGGCATCATCCGGCAAAGGTTGCCCGGCAAAGGCATGCAg                                                                                     <  2:2103758/100‑1 (MQ=255)
           tcACGCATGATCTCTTTTGCCGCTTCCGGGATTTCGTCGCTGCGCTCTTTACGCAGATCGGCATCATCCGGCAAAGGTTGCCCGGCAAAGGCATGCAGaa                                                                                   <  2:2555006/100‑1 (MQ=255)
             cgatctGATCTCTTTTGCCGCTTCCGGGATTTCGTCGCTGCGCTCTTTACGCAGATCGGCATCATCCGGCAAAGGTTGCCCGGTAAAGGCATGCAGAAAc                                                                                 <  1:850749/95‑1 (MQ=255)
             aCGCATGATCTCTTTTGCCGCTTCCGGGATTTCGTCGCTGCGCTCTTTACGCAGATCGGCATCATCCGGCAAAGGTTGCCCGGCAAAGGCATGCAGAAAc                                                                                 <  1:1415913/100‑1 (MQ=255)
              cGCATGATCTCTTTTGCCGCTTCCGGGATTTCGTCGCTGCGCTCTTTACGCAGATCGGCATCATCCGGCAAAGGTTGCCCGGCAAAGGCATGCAGAAACg                                                                                >  2:170634/1‑100 (MQ=255)
              cGCATGATCTCTTTTGCCGCTTCCGGGATTTCGACGCTGCGCTCTTTACGCAGATCCGCATCATCCGGCAAAGGATGCCTGGCAAAAGAATgacgttaa                                                                                 >  1:505848/1‑92 (MQ=255)
              cGCAGGATCTCTTTTGGCGCTTCCGGGATTTCGTCGCTGCGCTCGTTAGGCAGATCGGCATCATCCGGCAAAGGTTGCCCGGCAAAGGCATGCAGAAACg                                                                                <  1:2730141/100‑1 (MQ=255)
                  tGATCTCTTTTGCCGCTTCCGGGATTTCGTCGCTGCGCTCTTTACGCAGATCGGCATCATCCGGCAAAGGTTGCCCGGCAAAGGCATGCAGAAACGCTTc                                                                            >  2:2515963/1‑100 (MQ=255)
                   gATCTCTTTTGCGGCTTCCGGGATTTCGTCGCTGCGCTCTTTACGCAGATCGGCATCATCCGGCAAAGGTTGCCCGGCAAAGGCATGCAGAAACGCTTCg                                                                           <  2:528839/100‑1 (MQ=255)
                   gATCTCTTTTGCCGCTTCCGGGATTTCGTCGCTGCGCTCTTTACGCAGATCGGCATCATCCGGCAAAGGTTGCCCGGTAAAGGCATGCAGAAAcagatcg                                                                           >  2:850743/1‑94 (MQ=255)
                   gATCTCTTTTGCCGCTTCCGGGATTTCGTCGCTGCGCTCTTTACGCAGATCGGCATCATCCGGCAAAGGTTGCCCGGTAAAGGCATGCAGAAACGCTTCg                                                                           <  2:947709/100‑1 (MQ=255)
                   gATCTCTTTTGCCGCTTCCGGGATTTCGTCGCTGCGCTCTTTACGCAGATCGGCATCATCCGGCAAAGGTTGCCCGGCAAAGGCATGCAGAAACGCTTCg                                                                           >  2:925648/1‑100 (MQ=255)
                   gATCTCTTTTGCCGCTTCCGGGATTTCGTCGCTGCGCTCTTTACGCAGATCGGCATCATCCGGCAAAGGTTGCCCGGCAAAGGCATGCAGAAACGCTTCg                                                                           >  2:1649665/1‑100 (MQ=255)
                    aTCTCTTTTGCCGCTTCCGGGATTTCGTCGCTGCGCTCTTTACGCAGATCGGCATCATCCGGCAAAGGTTGCCCGGCAAAGGCATGCAGAAACGCTTCGc                                                                          <  2:190669/100‑1 (MQ=255)
                    aTCTCTTTTGCCGCTTCCGGGATTTCGTCGCTGCGCTCTTTACGCAGATCGGCATCATCCGGCAAAGGTTGCCCGGCAAAGGCATGCAGAAACGCTTCGc                                                                          <  1:1997404/100‑1 (MQ=255)
                     tctcTTTTGCCGCTTCCGGGATTTCGTCGCTGCGCTCTTTACGCAGATCGGCATCATCCGGCAAAGGTTGCCCGGCAAAGGCATGCAGAAACGCTTCGca                                                                         >  1:198231/1‑100 (MQ=255)
                      ctctTTTGCCGCTTCCGGGATTTCGTCGCTGCGCTCTTTACGCAGATCGGCATCATCCGGCAAAGGTTGCCCGGCAAAGGCATGCAGAAACGCTTCGcac                                                                        >  2:2716546/1‑100 (MQ=255)
                       tctTTTGCCGCTTCCGGGATTTCGTCGCTGCGCTCTTTACGCAGATCGGCATCATCCGGCAAAGGTTGCCCGGCAAAGGCATGCAGAAACGCTTCGcaca                                                                       <  1:1599045/100‑1 (MQ=255)
                       tctTTTGCCGCTTCCGGGATTTCGTCGCTGCGCTCTTTACGCAGATCGGCATCATCCGGCAAAGGTTGCCCGGCAAAGGCATGCAGAAACGCTTCGcaca                                                                       <  1:2274084/100‑1 (MQ=255)
                       tctTTTGCCGCTTCCGGGATTTCGTCGCTGCGCTCTTTACGCAGATCGGCATCATCCGGCAAAGGTTGCCCGGCAAAGGCATGCAGAAACGCTTCGcaca                                                                       >  2:2472077/1‑100 (MQ=255)
                         ttttGCCGCTTCCGGGATTTCGTCGCTGCGCTCTTTACGCAGATCGGCATCATCCGGCAAAGGTTGCCCGGCAAAGGCATGCAGAAACGCTTCGCAcagc                                                                     <  1:1428482/100‑1 (MQ=255)
                         ttttGCCGCTTCCGGGATTTCGTCGCTGCGCTCTTTACGCAGATCGGCATCATCCGGCAAAGGTTGCCCGGCAAAGGCATGCAGAAACGCTTCGCAcagc                                                                     <  2:615951/100‑1 (MQ=255)
                          tttGCCGCTTCCGGGATTTCGTCGCTGCGCTCTTTACGCAGATCGGCATCATCCGGCAAAGGTTGCCCGGCAAAGGCATGCAGAAACGCTTCGCAcagca                                                                    <  2:1988261/100‑1 (MQ=255)
                            tGCCGCTTCCGGGATTTCGTCGCTGCGCTCTTTACGCAGCTCGGCATCATCCGGCAAAGGTTGCCCGGCAAAGGCATGCAGAAACGCTTCGCACagcagc                                                                  >  2:1836206/1‑100 (MQ=255)
                              ccGCTTCCGGGATTTCGTCGCTGCGCTCTTTACGCAGATCGGCATCATCCGGCAAAGGTTGCCCGGCAAAGGCATGCAGAAACGCTTCGCACAGCAGCTc                                                                >  1:949473/1‑100 (MQ=255)
                                gCTTCCGGGATTTCGTCGCTGCGCTCTTTACGCAGATCGGCATCATCCGGCAAAGGTTGCCCGGCAAAGGCATGCAGAAACGCTTCGCACAGCAGCTCGc                                                              <  2:420584/100‑1 (MQ=255)
                                 cTTCCGGGATTTCGTCGCTGCGCTCTTTACGCAGATCGGCATCATCCGGCAAAGGTTGCCCGGCAAAGGCATGCAGAAACGCTTCGCACAGCAGCTCGCt                                                             >  1:931852/1‑100 (MQ=255)
                                 cTTCCGGGATTTCGTCGCTGCGCTCTTTACGCAGATCGGCATCATCCGGCAAAGGTTGCCCGGCAAAGGCATGCAGAAACGCTTCGCACAGCAGCTCGCt                                                             <  2:2319568/100‑1 (MQ=255)
                                   tCCGGGATTTCGTCGCTGCGCTCTTTACGCATATCGGCATCATCCGGCAAAGGTTGCCCGGCAAAGGCATGCAGAAACGCTTCGCACAGCAGCTCGCTg                                                            <  1:2632677/99‑1 (MQ=255)
                                   tCCGGGATTTCGTCGCTGCGCTCTTTACGCAGATCGGCATCATCCGGCAAAGGTTGCCCGGCAAAGGCATGCAGAAACGCTTCGCACAGCAGCTCGCTGt                                                           <  2:2101138/100‑1 (MQ=255)
                                   tCCGGGATTTCGTCGCTGCGCTCTTTACGCAGATCGGCATCATCCGGCAAAGGTTGCCCGGCAAAGGCATGCAGAAACGCTTCGCACAGCAGCTCGCTGt                                                           >  1:1518098/1‑100 (MQ=255)
                                     cGGGATTTCGTCGCTGCGCTCTTTACGCAGATCGGCATCATCCGGCAAAGGTTGCCCGGCAAAGGCATGCAGAAACGCTTCGCACAGCAGCTCGCTGTTg                                                         >  2:1411439/1‑100 (MQ=255)
                                      gggATTTCGTCGCTGCGCTCTTTACGCAGATCGGCATCATCCGGCAAAGGTTGCCCGGCAAAGGCATGCAGAAACGCTTCGCACAGCAGCTCGCTGTTgg                                                        <  1:1001888/100‑1 (MQ=255)
                                      gggATTTCGTCGCTGCGCTCTTTACGCAGATCGGCATCATCCGGCAAAGGTTGCCCGGCAAAGGCATGCAGAAACGCTTCGCACAGCAGCTCGCTGTTgg                                                        <  2:634120/100‑1 (MQ=255)
                                        gATTTCGTCGCTGCGCTCTTTACGCAGATCGGCATCATCCGGCAAAGGTTGCCCGGCAAAGGCATGCAGAAACGCTTCGCACAGCAGCTCGCTGTTGGTa                                                      <  1:1985432/100‑1 (MQ=255)
                                          tttCGTCGCTGCGCTCTTTACGCAGATCGGCATCATCCGGCAAAGGTTGCCCGGCAAAGGCATGCAGAAACGCTTCGCACAGCAGCTCGCTGTTGGTAGc                                                    <  2:1036664/100‑1 (MQ=255)
                                          tttCGTCGCTGCGCTCTTTACGCAGATCGGCATCATCCGGCAAAGGTTGCCCGGCAAAGGCATGCAGAAACGCTTCGCACAGCAGCTCGCTGTTGGTAGc                                                    >  1:1776357/1‑100 (MQ=255)
                                           ttCGTCGCTGCGCTCTTTACGCAGATCGGCATCATCCGGCAAAGGTTGCCCGGCAAAGGCATGCAGAAACGCTTCGCACAGCAGCTCGCTGTTGGTAGCg                                                   <  2:1432721/100‑1 (MQ=255)
                                             cgtcgCTGCGCTCTTTACGCAGATCGGCATCATCCGGCAAAGGTTGCCCGGCAAAGGCATGCAGAAACGCTTCGCACAGCAGCTCGCTGTTGGTAGCGTg                                                 >  2:660519/1‑100 (MQ=255)
                                             cgtcgCTGCGCTCTTTACGCAGATCGGCATCATCCGGCAAAGGTTGCCCGGCAAAGGCATGCAGAAACGCTTCGCACAGCAGCTCGCTGTTGGTAGCGTg                                                 <  1:1786165/100‑1 (MQ=255)
                                             cgtcgCTGCGCTCTTTACGCAGATCGGCATCATCCGGCAAAGGTTGCCCGGCAAAGGCATGCAGAAACGCTTCGCACAGCAGCTCGCTGTTGGTAGCGTg                                                 <  2:2525696/100‑1 (MQ=255)
                                             cgtcgCTGCGCTCTTTACGCACATCGGCATCATCCGGCAAAGGTTGCCCGGCAAAGGCATGCAGAAATGCTTCGCACAGCAGCTCGCTGTTGGTAGCGTg                                                 <  1:2623150/100‑1 (MQ=255)
                                               tcgCTGCGCTCTTTACGCAGATCGGCATCATCCGGCAAAGGTTGCCCGGCAAAGGCATGCAGAAACGCTTCGCACAGCAGCTCGCTGTTGGTAGCGTGAc                                               <  2:868113/100‑1 (MQ=255)
                                                 gCTGCGCTCTTTACGCAGATCGGCATCATCCGGCAAAGGTTGCCCGGCAAAGGCATGCAGAAACGCTTCGCACAGCAGCTCGCTGTTGGTAGCGTGACGc                                             >  1:1920317/1‑100 (MQ=255)
                                                  cTGCGCTCTTTACGCAGCTCGGCATCATCCGGCAAAGGTTGCCCGGCAAAGGCATGCAGAAACGCTTCGCACAGCAGCTCGCTGTTGGTAGCGTGACGCa                                            <  1:1068504/100‑1 (MQ=255)
                                                  cTGCGCTCTTTACGCAGATCGGCATCATCCGGCAAAGGTTGCCCGGCAAAGGCATGCAGAAACGCTTCGCACAGCAGCTCGCTGTTGGTAGCGTGACGCa                                            <  2:625169/100‑1 (MQ=255)
                                                  cTGCGCTCTTTACGCAGATCGGCATCATCCGGCAAAGGTTGCCCGGCAAAGGCATGCAGAAACGCTTCGCACAGCAGCTCGCTGTTGGTAGCGTGACGCa                                            <  2:2006049/100‑1 (MQ=255)
                                                     cgcTCTTTACGCAGATCGGCATCATCCGGCAAAGGTTGCCCGGCAAAGGCATGCAGAAACGCTTCGCACAGCAGCTCGCTGTTGGTAGCGTGACGCAGgt                                         >  1:1967626/1‑100 (MQ=255)
                                                        tctTTACGCAGATCGGCATCATCCGGCAAAGGTTGCCCGGCAAAGGCATGCAGAAACGCTTCGCACAGCAGCTCGCTGTTGGTAGCGTGACGCAGgttgt                                      >  2:2323420/1‑100 (MQ=255)
                                                           ttACGCAGATCGGCATCATCCGGCAAAGGTTGCCCGGCAAAGGCATGCAGAAACGCTTCGCACAGCAGCTCGCTGTTGGTAGCGTGACGCAGGTTGTTCa                                   <  2:2315380/100‑1 (MQ=255)
                                                            tACGCAGATCGGCATCATCCGGCAAAGGTTGCCCGGCAAAGGCATGCAGAAACGCTTCGCACAGCAGCTCGCTGTTGGTAGCGTGACGCAGGTTGTTCAc                                  >  2:2031387/1‑100 (MQ=255)
                                                            tACGCAGATCGGCATCATCCGGCAAAGGTTGCCCGGCAAAGGCATGCAGAAACGCTTCGCACAGCAGCTCGCTGTTGGTAGCGTGACGCAGGTTGTTCAc                                  >  2:669929/1‑100 (MQ=255)
                                                            tACGCAGATCGGCATCATCCGGCAAAGGTTGCCCGGCAAAGGCATGCAGAAACGCTTCGCACAGCAGCTCGCTGTTGGTAGCATGACGCAGGTTGTTCAc                                  >  2:672999/1‑100 (MQ=255)
                                                             aCGCAGATCGGCATCATCCGGCAAAGGTTGCCCGGCAAAGGCATGCAGAAACGCTTCGCACAGCAGCTCGCTGTTGGTAGCGTGACGCAGGTTGTTCAcc                                 <  1:2398773/100‑1 (MQ=255)
                                                              cGCAGATCGGCATCATCCGGCAAAGGTTGCCCGGCAAAGGCATGCAGAAACGCTTCGCACAGCAGCTCGCTGTTGGTAGCGTGACGCAGGTTGTTCACCt                                >  1:2086347/1‑100 (MQ=255)
                                                              cGCAGATCGGCATCATCCGGCAAAGGTTGCCCGGCAAAGGCATGCAGAAACGCTTCGCACAGCAGCTCGCTGTTGGTAGCGTGACGCAGGTTGTTCACCt                                >  2:2578488/1‑100 (MQ=255)
                                                              cGCAGATCGGCATCATCCGGCAAAGGTTGCCCGGCAAAGGCATGCAGAAACGCTTCGCACAGCAGCTCGCTGTTGGTAGCGTGACGCAGGTTGTTCACCt                                >  1:137887/1‑100 (MQ=255)
                                                              cGCAGATCGGCATCATCCGGCAAAGGTTGCCCGGCAAAGGCATGCAGAAACGCTTCGCACAGCAGCTCGCTGTTGGTAGCGTGACGCAGGTTGTTCACCt                                <  1:1553297/100‑1 (MQ=255)
                                                              cGCAGATCGGCATCATCCGGCAAAGGTTGCCCGGCAAAGGCATGCAGAAACGCTTCGCACAGCAGCTCGCTGTTGGTAGCGTGACGCAGGTTGTTCACCt                                >  1:1421237/1‑100 (MQ=255)
                                                               gCAGATCGGCATCATCCGGCAAAGGTTGCCCGGCAAAGGCATGCAGAAACGCTTCGCACAGCAGCTCGCTGTTGGTAGCGTGACGCAGGTTGTTCACCTg                               <  1:1413485/100‑1 (MQ=255)
                                                                cAGATCGGCATCATCCGGCAAAGGTTGCCCGGCAAAGGCATGCAGAAACGCTTCGCACAGCAGCTCGCTGTTGGTAGCGTGACGCAGGTTGTTCACCTga                              >  2:388494/1‑100 (MQ=255)
                                                                 aGATGGGCATCATCCGGCAAAGGTTGCCCGGCAAAGGCATGCAGAAACGCTTCGCACAGCAGCTCGCTGTTGGTAGCGTGACGCAGGTTGTTCACCTgac                             <  1:691370/100‑1 (MQ=255)
                                                                 aGATCGGCATCATCCGGCAAAGGTTGCCCGGCAAAGGCATGCAGAAACGCTTCGCACAGCAGCTCGCTGTTGGTAGCGTGACGCAGGTTGTTCACCTgac                             >  1:1244948/1‑100 (MQ=255)
                                                                 aGATCGGCATCATCCGGCAAAGGTTGCCCGGCAAAGGCATGCAGAAACGCTTCGCACAGCAGCTCGCTGTTGGTAGCGTGACGCAGGTTGTTCACCTgac                             <  1:925654/100‑1 (MQ=255)
                                                                    tCGGCATCATCCGGCAAAGGTTGCCCGGCAAAGGCATGCAGAAACGCTTCGCACAGCAGCTCGCTGTTGGTAGCGTGACGCAGGTTGTTCACCTgacgac                          >  2:1845294/1‑100 (MQ=255)
                                                                     cGGCATCATCCGGCAAAGGTTGCCCGGCAAAGGCATGCAGAAACGCTTCGCACAGCAGCTCGCTGTTGGTAGCGTGACGCAGGTTGTTCACCTGacgacg                         <  2:2209334/100‑1 (MQ=255)
                                                                     cGGCATCATCCGGCAAAGGTTGCCCGGCAAAGGCATGCAGAAACGCTTCGCACAGCAGCTCGCTGTTGGTAGCGTGACGCAGGTTGTTCACCTGacgacg                         <  1:2515982/100‑1 (MQ=255)
                                                                     cGGCATCATCCGGCAAAGGTTGCCCGGCAAAGGCATGCAGAAACGCTTCGCACAGCAGCTCGCTGTTGGTAGCGTGACGCAGGTTGTTCACCTGacgacg                         <  1:2192676/100‑1 (MQ=255)
                                                                       gCATCATCCGGCAAAGGTTGCCCGGCAAAGGCATGCAGAAACGCTTCGCACAGCAGCTCGCTGTTGGTAGCGTGACGCAGGTTGTTCACCTGACGAcgcg                       >  2:877743/1‑100 (MQ=255)
                                                                       gCATCATCCGGCAAAGGTTGCCCGGCAAAGGCATGCAGAAACGCTTCGCACAGCAGCTCGCTGTTGGTAGCGTGACGCAGGTTGTTCACCTGACGAcgcg                       <  2:1446324/100‑1 (MQ=255)
                                                                         atcatcCGGCAAAGGTTGCCCGGCAAAGGCATGCAGAAACGCTTCGCACAGCAGCTCGCTGTTGGTAGCGTGACGCAGGTTGTTCACCTGACGACgcgtg                     <  1:2031404/100‑1 (MQ=255)
                                                                           catcCGGCAAAGGTTGCCCGGCAAAGGCATGCAGAAACGCTTCGCACAGCAGCTCGCTGTTGGTAGCGTGACGCAGGTTGTTCACCTGACGACgcgtgcg                   >  1:1701116/1‑100 (MQ=255)
                                                                           catcCGGCAAAGGTTGCCCGGCAAAGGCATGCAGAAACGCTTCGCACAGCAGCTCGCTGTTGGTAGCGTGACGCAGGTTGTTCACCTGACGACgcgtgcg                   <  2:544933/100‑1 (MQ=255)
                                                                           atctcGGCAAAGGTTGCCCGGCAAAGGCATGCAGAAACGCTTCGCACAGCAGCTCGCTGTTGGTAGCGTGACGCAGGTTGTTCACCTGACGACgcgtgcg                   <  1:186415/96‑1 (MQ=255)
                                                                            atcCGGCAAAGGTTGCCCGGCAAAGGCATGCAGAAACGCTTCGCACAGCAGCTCGCTGTTGGTAGCGTGACGCAGGTTGTTCACCTGACGACgcgtgcgt                  >  2:1571157/1‑100 (MQ=255)
                                                                            atcCGGCAAAGGTTGCCCGGCAAAGGCATGCAGAAACGCTTCGCACAGCAGCTCGCTGTTGGTAGCGTGACGCAGGTTGTTCACCTGACGACgcgtgcgt                  >  1:414748/1‑100 (MQ=255)
                                                                            atcCGGCAAAGGTTGCCCGGCAAAGGCATGCAGAAACGCTTCGCACAGCAGCTCGCTGTTGGTAGCGTGACGCAGGTTGTTCACCTGACGACgcgtgcgt                  >  1:1156674/1‑100 (MQ=255)
                                                                             tcCGGCATAGGTTGCCCGGCAAAGGCATGCAGAAACGCTTCGCACAGCAGCTCGCTGTTGGTAGCGTGACGCAGGTTGTTCACCTGACGACGCGTGCGtt                 >  1:1458068/1‑100 (MQ=255)
                                                                             tcCGGCAAAGGTTGCCCGGCAAAGGCATGCAGAAACGCTTCGCACAGCAGCTCGCTGTTGGTAGCGTGACGCAGGTTGTTCACCTGACGACGCGTGCGtt                 <  1:2646607/100‑1 (MQ=255)
                                                                             tcCGGCAAAGGTTGCCCGGCAAAGGCATGCAGAAACGCTTCGCACAGCAGCTCGCTATTGGTAGCGTGACGCAGGTTGTTCACCTGACGACGCGTGCGtt                 >  2:1769369/1‑100 (MQ=255)
                                                                                ggCAAAGGTTGCCCGGCAAAGGCATGCAGAAACGCTTCGCACAGCAGCTCGCTGTTGGTAGCGTGACGCAGGTTGTTCACCTGACGACGCGTGCGTTCAt              >  1:388763/1‑100 (MQ=255)
                                                                                ggCAAAGGTTGCCCGGCAAAGGCATGCAGAAACGCTTCGCACAGCAGCTCGCTGTTGGTAGCGTGACGCAGGTTGTTCACCTGACGACGCGTGCGTTCAt              >  2:2155365/1‑100 (MQ=255)
                                                                                 gCAAAGGTTGCCCGGCAAAGGCATGCAGAAACGCTTCGCACAGCAGCTCGCTGTTGGTAGCGTGACGCAGGTTGTTCACCTGACGACGCGTGCGTTCATc             <  1:1160049/100‑1 (MQ=255)
                                                                                  cAAAGGTTGCCCGGCAAAGGCATGCAGAAACGCTTCGCACAGCAGCTCGCTGTTGGTAGCGTGACGCAGGTTGTTCACCTGACGACGCGTGCGTTCATCg            >  1:216963/1‑100 (MQ=255)
                                                                                  cAAAGGTTGCCCGGCAAAGGCATGCAGAAACGCTTCGCACAGCAGCTCGCTGTTGGTAGCGTGACGCAGGTTGTTCACCTGACGACGCGTGCGTTCATCg            >  1:1646706/1‑100 (MQ=255)
                                                                                   aaaGGTTGCCCGGCAAAGGCATGCAGAAACGCTTCGCACAGCAGCTCGCTGTTGGTAGCGTGACGCAGGTTGTTCACCTGACGACGCGTGCGTTCATCgg           <  1:648052/100‑1 (MQ=255)
                                                                                   aaaGGTTGCCCGGCAAAGGCATGCAGAAACGCTTCGCACAGCAGCTCGCTGTTGGTAGCGTGACGCAGGTTGTTCACCTGACGACGCGTGCGTTCATCgg           <  1:663177/100‑1 (MQ=255)
                                                                                      ggTTGCCCGGCAAAGGCATGCAGAAACGCTTCGCACAGCAGCTCGCTGTTGGTAGCGTGACGCAGGTTGTTCACCTGACGACGCGTGCGTTCATCGGTGa        >  1:1445921/1‑100 (MQ=255)
                                                                                       gTTGCCCGGCAAAGGCATGCAGAAACGCTTCGCACAGCAGCTCGCTGTTGGTAGCGTGACGCAGGTTGTTCACCTGACGACGCGTGCGTTCATCGGTGAg       >  1:1529969/1‑100 (MQ=255)
                                                                                        ttGCCCGGCAAAGGCATGCAGAAACGCTTCGCACAGCAGCTCGCTGTTGGTAGCGTGACGCAGGTTGTTCACCTGACGACGCGTGCGTTCATCGGTGAgg      <  2:503259/100‑1 (MQ=255)
                                                                                         tGCCCGGTAAAGGCATGCAGAAACGCTTCGCACAGCAGCTCGCTGTTGGTAGCGTGACGCAGGTTGTTCACCTGACGACGCGTGCGTTCATCGGTGAGGa     <  1:1657937/100‑1 (MQ=255)
                                                                                           cccGGCAAAGGCATGCAGAAACGCTTCGCACAGCAGCTCGCTGTTGGTAGCGTGACGCAGGTTGTTCACCTGACGACGCGTGCGTTCATCGGTGAGGAtt   >  1:1499666/1‑100 (MQ=255)
                                                                                            ccGGCAAAGGCATGCAGAAACGCTTCGCACAGCAGCTCGCTGTTGGTAGCGTGACGCAGGTTGTTCACCTGACGACGCGTGCGTTCATCGGTGAGGAttt  >  1:446385/1‑100 (MQ=255)
                                                                                                |                                                                                               
TAATCCCCATCTCACGCATGATCTCTTTTGCCGCTTCCGGGATTTCGTCGCTGCGCTCTTTACGCAGATCGGCATCATCCGGCAAAGGTTGCCCGGTAAAGGCATGCAGAAACGCTTCGCACAGCAGCTCGCTGTTGGTAGCGTGACGCAGGTTGTTCACCTGACGACGCGTGCGTTCATCGGTGAGGATTT  >  NC_000913/4128101‑4128292

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A