Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A8 F232 I0 R1 243 156.3 7646460 93.8% 7172379 99.8

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 460,106 A→T 100% I407F (ATC→TTC)  lon → DNA‑binding ATP‑dependent protease La

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913460,1060AT99.0% 394.0 / ‑4.2 125I407F (ATC→TTC) lonDNA‑binding ATP‑dependent protease La
Reads supporting (aligned to +/- strand):  ref base A (2/0);  new base T (52/70);  total (54/71)
Fisher's exact test for biased strand distribution p-value = 1.88e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 125 total aligned reads displayed.

GTAAATATGTCCGTATGGCGCTGGGCGGCGTGCGTGATGAAGCGGAAATCCGTGGTCACCGCCGTACTTACATCGGTTCTATGCCGGGTAAACTGATCCAGAAAATGGCGAAAGTGGGCGTGAAAAACCCGCTGTTCCTGCTCGATGAGATCGACAAAATGTCTTCTGACATGCGTGGCGATCCGGCCTCTG  >  NC_000913/460011‑460202
                                                                                               |                                                                                                
gTAAATATGTCCGTATGGCGCTGGGCGGCGTGCGTGATGAAGCGGAAATCGGTGATCACCGCCGTACTTACATCGGTTCTATGCCGGGTAAACTGTTCCa                                                                                              >  1:979629/1‑100 (MQ=255)
gTAAATATGTCCGTATGGCGCTGGGCGGCGTGCGTGATGAAGCGGAAATCCGTGGTCACCGCCGTACTTACATCGGTTCTATGCCGGGTAAACTGTTcc                                                                                               <  2:1445362/99‑1 (MQ=255)
gTAAATATGTCCGTATGGCGCTGGGCGGCGTGCGTGATGAAGCGGAAATCCGTGGTCACCGCCGTACTTACATCGGTTCTATGCCGGGTAAACTGTTCCa                                                                                              <  1:3089896/100‑1 (MQ=255)
   aaTATGTCCGTATGGCGCTGGGCGGCGTGCGTGATGAAGCGGAAATCCGTGGTCACCGCCGTACTTACATCGGTTCTATGCCGGGTAAACTGTTCCAGaa                                                                                           >  1:2520901/1‑100 (MQ=255)
    atatGTCCGTATGGCGCTGGGCGGCGTGCGTGATGAAGCGGAAATCCGTGGTCACCGCCGTACTTACATCGGTTCTATGCCGGGTAAACTGTTCCAGaaa                                                                                          <  2:1329792/100‑1 (MQ=255)
    atatGTCCGTATGGCGCTGGGCGGCGTGCGTGATGAAGCGGAAATCCGTGGTCACCGCCGTACTTACATCGGTTCTATGCCGGGTAAACTGTTCCAGaaa                                                                                          >  1:1420714/1‑100 (MQ=255)
    atatGTCCGTATGGCGCTGGGCGGCGTGCGTGATGAAGCGGAAATCCGTGGTCACCGCCGTACTTACATCGGTTCTATGCCGGGTAAACAGATcggaaga                                                                                          >  2:3232771/1‑94 (MQ=255)
      atGTCCGTATGGCGCTGGGCGGCGTGCGTGATGAAGCGGAAATCCGTGGTCACCGCCGTACTTACATCGGTTCTATGCCGGGTAAACTGTTCCAGAAAAt                                                                                        <  2:2870246/100‑1 (MQ=255)
      atGTCCGTATGGCGCTGGGCGGCGTGCGTGATGAAGCGGAAATCCGTGGTCACCGCCGTACTTACATCGGTTCTATGCCGGGTAAACTGTTCCAGAAAAt                                                                                        <  1:1903452/100‑1 (MQ=255)
       tGTCCGTATGGCGCTGGGCGGCGTGCGTGATGAAGCGGAAATCCGTGGTCACCGCCGTACTTACATCGGTTCTATGCCGGGTAAACTGTTCCAGAAAATg                                                                                       >  1:2929767/1‑100 (MQ=255)
        gTCCGTATGGCGCTGGGCGGCGTGCGTGATGAAGCGGAAGTCCGTGGTCACCGCCGTACTTACATCGGTTCTATGCCGGGTAAACTGTTCCAGAAAAt                                                                                        <  2:1254697/98‑1 (MQ=255)
        gTCCGTATGGCGCTGGGCGGCGTGCGTGATGAAGCGGAAATCGGTGGTGACGGCCGTAATTACATCGGTTCTGGGACGGGGAAACTGTTCCGGCAAAAgg                                                                                      >  2:3315058/1‑100 (MQ=255)
         tCCGTATGGCGCTGGGCGGCGTGCGTGATGAAGCGGAAATCCGTGGTCACCGCCGTACTTACATCGGTTCTATGCCGGGTAAACTGTTCCAGAAAATGGc                                                                                     <  2:2758478/100‑1 (MQ=255)
         tCCGTATGGCGCTGGGCGGCGTGCGTGATGAAGCGGAAATCCGTGGTCACCGCCGTACTTACATCGGTTCTATGCCGGGTAAACTGTTCCAGAAAATGGc                                                                                     >  1:298931/1‑100 (MQ=255)
          ccGTATGGCGCTGGGCGGCGTGCGTGATGAAGCGGAAATCGGTGGACACCGCCGTACTTACATCGGTTCTATGCCGGGTAAACTGTTCCAGAAAATgg                                                                                      <  2:2671786/98‑1 (MQ=255)
          ccGTATGGCGCTGGGCGGCGTGCGTGATGAAGCGGAAATCCGTGGTCACCGCCGTACTTACATCGGTTCTATGCCGGGTAAACTGTTCCAGAAAATGGCg                                                                                    >  2:3004513/1‑100 (MQ=255)
             tATGGCGCTGGGCGGCGTGCGTGATGAAGCGGAAATCCGTGGTCACCGCCGTACTTACATCGGTTCTATGCCGGGTAAACTGTTCCAGAAAATGGCGaaa                                                                                 >  2:1915004/1‑100 (MQ=255)
             tATGGCGCTGGGCGGCGTGCGTGATGAAGCGGAAATCCGTGGTCACCGCCGTACTTACATCGGTTCTATGCCGGGTAAACTGTTCCAGAAAATGGCGaaa                                                                                 >  1:1681999/1‑100 (MQ=255)
                ggCGCTGGGCGGCGTGCGTGATGAAGCGGAAATCCGTGGTCGCCGCCGTACTTACATCGGTTCTATGCCGGGTAAACTGTTCCAGAAAATGGCGAAAGTg                                                                              <  2:3213094/100‑1 (MQ=255)
                ggCGCTGGGCGGCGTGCGTGATGAAGCGGAAATCCGTGGTCACCGCCGTACTTACATCGGTTCTATGCCGGGTAAACTGTTCCAGAAAATGGCGAAAGTg                                                                              >  1:481452/1‑100 (MQ=255)
                  cgcTGGGCGGCGTGCGTGATGAAGCGGAAATCCGTGGTCACCGCCGTACTTACATCGGTTCTATGCCGGGTAAACTGTTCCAGAAAATGGCGAAAGTggg                                                                            <  1:2180300/100‑1 (MQ=255)
                   gcTGGGCGGCGTGCGTGATGAAGCGGAAATCCGTGGTCACCGCCGTACTTACATCGGTTCTATGCCGGGTAAACTGTTCCAGAAAATGGCGAAAGTGGGc                                                                           >  1:1183945/1‑100 (MQ=255)
                    cTGGGCGGCGTGCGTGATGAAGCGGAAATCCGTGGTCACCGCCGTACTTACATCGGTTCTATGCCGGGTAAACTGTTCCAGAAAATGGCGAAAGTGGGCg                                                                          <  2:2121733/100‑1 (MQ=255)
                      gggCGGCGTGCGTGATGAAGCGGAAATCCGTGGTCACCGCCGTCCTTACATCGGTTCTATGCCGGGTAAACTGTTCCAGAAAATGGCGAAAGTGGGCGTg                                                                        >  2:3567057/1‑100 (MQ=255)
                        gcggcgTGCGTGATGAAGCGGAAATCCGTGGTCACCGCTGTACTTACATCGGTTCTATGCCGGGTAAACTGTTCCAGAAAATGGCGAAAGTGGGCGTGaa                                                                      >  2:1999365/1‑100 (MQ=255)
                         cggcgTGCGTGATGAAGCGGAAATCCGTGGTCACCGCCGTACTTACATCGGTTCTATGCCGGGTAAACTGTTCCAGAAAATGGCGAAAGTGGGCGTGaaa                                                                     <  2:1052638/100‑1 (MQ=255)
                           ncgtgcgtgATGAAGCGGAAATCCGTGGTCACCGCCGTACTTACATCGGTTCTATGCCGGGTAAACTGTTCCAGAAAATGGCGAAAGTGGGCGTGaaaaa                                                                   >  2:282843/2‑100 (MQ=255)
                           gcgtgcgtGATGAAGCGGAAATCCGTGGTCACCGCCGTACTTACATCGGTTCTATGCCGGGTAAACTGTTCCAGAAAATGGCGAAAGTGGGCGTGaaaaa                                                                   >  2:2139552/1‑100 (MQ=255)
                             gtgcgtgATGAAGCGGAAATCCGTGGTCACCGCCGTACTTACATCGGTTCTATGCCGGGTAAACTGTTCCAGAAAATGGCGAAAGTGGGCGTGAAAAAcc                                                                 <  1:2871594/100‑1 (MQ=255)
                              tgcgtgATGAAGCGGAAATCCGTGGTCACCGCCGTACTTACATCGGTTCTATGCCGGGTAAACTGTTCCAGAAAATGGCGAAAGTGGGCGTGAAAAAccc                                                                >  1:3790731/1‑100 (MQ=255)
                               gcgtgATGAAGCGGAAATCCGTGGTCACCGCCGTACTTACATCGGTTCTATGCCGGGTAAACTGTTCCAGAAAATGGCGAAAGTGGGCGTGAAAAACCCg                                                               <  2:45016/100‑1 (MQ=255)
                                 gtgATGAAGCGGAAATCCGTGGTCACCGCCGTACTTACATCGGTTCTATGCCGGGTAAACTGTTCCAGAAAATGGCGAAAGTGGGCGTGAAAAACCCGCt                                                             >  2:925051/1‑100 (MQ=255)
                                 gtgATGAAGCGGAAATCCGTGGTCACCGCCGTACTTACATCGGTTCTATGCCGGGTAAACTGTTCCAGAAAATGGCGAAAGTGGGCGTGAAAAACCCGCt                                                             <  2:1973668/100‑1 (MQ=255)
                                  tgatgaAGCGGAAATCCGTGGTCACCGCCGTACTTACATCGGTTCTATGCCGGGTAAACTGTTCCAGAAAATGGCGAAAGTGGGCGTGAAAAACCCGCTg                                                            <  1:1479582/100‑1 (MQ=255)
                                  tgatgaAGCGGAAATCCGTGGTCACCGCCGTACTTACATCGGTTCTATGCCGGGTAAACTGTTCCAGAAAATGGCGAAAGTGGGCGTGAAAAACCCGCTg                                                            <  1:986715/100‑1 (MQ=255)
                                    atgaAGCGGAAATCCGTGGTCACCGCCGTACTTACATCGGTTCTATGCCGGGTAAACTGTTCCAGAAAATGGCGAAAGTGGGCGTGAAAAACCCGCTGtt                                                          <  2:1659572/100‑1 (MQ=255)
                                      gaAGCGGAAATCCGTGGTCACCGCCGTACTTACATCGGTTCTATGCCGGGTAAACTGTTCCAGAAAATGGCGAAAGTGGGCGTGAAAAACCCGCTGTTcc                                                        <  1:1418192/100‑1 (MQ=255)
                                      gaAGCGGAAATCCGTGGTCACCGCCGTACTTACATCGGTTCTATGCCGGGTAAACTGTTCCAGAAAATGGCGAAAGTGGGCGTGAAAAACCCGCTGTTcc                                                        <  1:1703053/100‑1 (MQ=255)
                                        aGCGGAAATCCGTGGTCACCGCCGTACTTACATCGGTTCTATGCCGGGTAAACTGTTCCAGAAAATGGCGAAAGTGGGCGTGAAAAACCCGCTGTTCCTg                                                      >  1:336850/1‑100 (MQ=255)
                                        aGCGGAAATCCGTGGTCACCGCCGTACTTACATCGGTTCTATGCCGGGTAAACTGTTCCAGAAAATGGCGAAAGTGGGCGTGAAAAACCCGCTGTTCCTg                                                      >  1:3541709/1‑100 (MQ=255)
                                        aGCGGAAATCCGTGGTCACCGCCGTACTTACATCGGTTCTATGCCGGGTAAACTGTTCCAGAAAATGGCGAAAGTGGGCGTGAAAAACCCGCTGTTCCTg                                                      >  1:1282813/1‑100 (MQ=255)
                                        aGCGGAAATCCGTGGTCACCGCCGTACTTACATCGGTTCTATGCCGGGTAAACTGTTCCAGAAAATGGCGAAAGTGGGCGTGAAAAACCCGCTGTTCCTg                                                      >  2:834200/1‑100 (MQ=255)
                                        aGCGGAAATCCGTGGTCACCGCCGTACTTACATCGGTTCTATGCCGGGTAAACTGATCCAGAACATGGCGAAAGTGGGCGCGAAAAACCCGCTGTTcc                                                        >  2:3637281/1‑98 (MQ=255)
                                         gCGGAAATCCGTGGTCACCGCCGTACTTACATCGGTTCTATGCCGGGTAAACTGTTCCAGAAAATGGCGAAAGTGGGCGTGAAAAACCCGCTGTTCCTGc                                                     <  1:1145628/100‑1 (MQ=255)
                                         gCGGAAATCCGTGGTCACCGCCGTACTTACATCGGTTCTATGCCGGGTAAACTGTTCCAGAAAATGGCGAAAGTGGGCGTGAAAAACCCGCTGTTCCTGc                                                     <  2:3798793/100‑1 (MQ=255)
                                         gCGGAAATCCGTGGTCACCGCCGTACTTACATCGGTTCTATGCCGGGTAAACTGTTCCAGAAAATGGCGAAAGTGGGCGTGAAAAACCCGCTGTTCCTGc                                                     <  1:1805601/100‑1 (MQ=255)
                                          cGGAAATCCGTGGTCACCGCCGTACTTACATCGGTTCTATGCCGGGTAAACTGTTCCAGAAAATGGCGAAAGTGGGCGTGAAAAACCCGCTGTTCCTGCt                                                    <  1:1690561/100‑1 (MQ=255)
                                          cGGAAATCCGTGGTCACCGCCGTACTTACATCGGTTCTATGCCGGGTAAACTGTTCCAGAAAATGGCGAAAGTGGGCGTGAAAAACCCGCTGTTCCTGCt                                                    <  1:2142600/100‑1 (MQ=255)
                                          cGGAAATCCGTGCTCACCGCCGTACTTACATCGGTTCTATGCCGGGTAAACGGTTCCAGAAAATGGCGAAAGTGGGCGGGAAATAACCGCGATTCCTGc                                                     >  2:2489467/1‑99 (MQ=255)
                                           ggAAATCCGTGGTCACCGCCGTACTTACATCGGTTCTATGCGGGGTAAACTGTTCCAGAAAATGGCGAAAGTGGGCGTGAAAAACCCGCTGTTCCTGc                                                     <  2:1524483/98‑1 (MQ=255)
                                           ggAAATCCGTGGTCACCGCCGTACTTACATCGGTTCTATGCCGGGTAAACTGTTCCAGAAAATGGCGAAAGTGGGCGTGAAAAACCCGCTGTTCCTGCTc                                                   <  2:3501774/100‑1 (MQ=255)
                                            gAAATCCGTGTTCACCGCCGTACTTACATCGGTTCTATGCCGGGTAAACTGTTCCAGAAAATGGCGAAAGTGGGCGTCAAAAACCCGCTGTTCCTGCTct                                                  <  2:3403892/100‑2 (MQ=255)
                                            gAAATCCGTGGTCACCGCCGTACTTACATCGGTTCTATGCCGGGTAAACTGTTCCAGAAAATGGCGAAAGTGGGCGTGAAAAACCCGCTGTTCCTGCTCg                                                  <  1:3250717/100‑1 (MQ=255)
                                              aaTCCGTGGTCACCGCCGTACTTACATCGGTTCTATGCCGGGTAAACTGTCCCAGAAAATGGCGAAAGTGGGCGTGAAAAACCCGCTGTTCCTGCTCGAt                                                <  2:3127183/100‑1 (MQ=255)
                                               aTCCGTGGTCACCGCCGTACTTACATCGGTTCTATGCCGGGTAAACTGTTCCAGAAAATGGCGAAAGTGGGCGTGAAAAACCCGCTGTTCCTGCTCGATg                                               <  1:2240150/100‑1 (MQ=255)
                                                tCCGTGGTCACCGCCGTACTTACATCGGTTCTATGCCGGGTAAACTGTTCCAGAAAATGGCGAAAGTGGGCGTGAAAAACCCGCTGTTCCTGCTCGATga                                              <  2:324450/100‑1 (MQ=255)
                                                 ccGTGGTCACCGCCGTACTTACATCGGTTCTATGCCGGGTAAACTGTTCCAGAAAATGGCGAAAGTGGGCGTGAAAAACCCGCTGTTCCTGCTCGATgag                                             >  2:3215556/1‑100 (MQ=255)
                                                   gTGGTCCCTGCGTTACTGAAATCGTTTCTATGCCGGGTAAACGGTTCCGTAAAAGGGCGAAGGTGGCCGTGAACAACCCGCTGTTCCTGCTcaa                                                 <  2:1411457/94‑3 (MQ=255)
                                                   gTGGTCACCGCCGTACTTCCATCGGTTCTATGCCGGGTAAACTGTTCCAGAAAATGGCGAAAGTGGGCGTGAAAAACCCGCTGTTCCTGCTCGATGAGAt                                           >  1:2816802/1‑100 (MQ=255)
                                                    tGGTCACCGCCGTACTTACATCGGTTCTATGCCGGGTAAACTGTTCCAGAAAATGGCGAAAGTGGGCGTGAAAAACCCGCTGTTCCTGCTCGATGAGATc                                          <  1:3771035/100‑1 (MQ=255)
                                                     ggTCACCGCCGTACTTACATCGGTTCTATGCCGGGTAAACTGTTCCAGAAAATGGCGAAAGTGGGCGTGAAAAACCCGCTGTTCCTGCTCGATGAGATCg                                         >  2:684067/1‑100 (MQ=255)
                                                     ggTCACCGCCGTACTTACATCGGTTCTATGCCGGGTAAACTGTTCCAGAAAATGGCGAAAGTGGGCGTGAAAAACCCGCTGTTCCTGCTCGATGAGATCg                                         <  1:1694306/100‑1 (MQ=255)
                                                     ggTCACAGCCGTACTTACATCGGTTCTATGCCGGGTAAAGTGTTCCAGAAAATGGCGAAAGTGGGCGTGAAAAACCCGCTGTTCCTGCTCGATGAGATCg                                         <  2:1272372/100‑1 (MQ=255)
                                                       tCACCGCCGTACTTACATCGGTTCTATGCCGGGTAAACTGCTCCAGAAAATGGCGAAAGTGGGCGTGAAAAACCCGCTGTTCCTGCTCGATGAGATCGAc                                       <  2:3457617/100‑1 (MQ=255)
                                                        cACCGCCGTACTTACATCGGTTCTATGCCGGGTAAACTGTTCCAGAAAATGGCGAAAGTGGGCGTGAAAAACCCGCTGTTCCTGCTCGATGAGATCGACa                                      >  2:2551749/1‑100 (MQ=255)
                                                         aCCGCCGTACTTACATCGGTTCTATGCCGGGTAAACTGTTCCAGAAAATGGCGAAAGTGGGCGTGAAAAACCCGCTGTTCCTGCTCGATGAGATCGACaa                                     <  1:2824012/100‑1 (MQ=255)
                                                          ccgccgTACTTACATCGGTTCTATGCCGGGTAAACTGTTCCAGAAAATGGCGAAAGTGGGCGTGAAAAACCCGCTGTTCCTGCTCGATGAGATCGACaaa                                    >  1:66100/1‑100 (MQ=255)
                                                           cgccgTACTTACATCGGTTCTATGCCGGGTAAACTGTTCCAGAAAATGGCGAAAGTGGGCGTGAAAAACCCGCTGTTCCTGCTCGATGAGATCGACaaaa                                   <  1:3088736/100‑1 (MQ=255)
                                                           cgccgTACTTACATCGGTTCTATGCCGGGTAAACTGTTCCAGAAAATGGCGAAAGTGGGCGTGAAAAACCCGCTGTTCCTGCTCGATGAGATCGACaaaa                                   >  1:190473/1‑100 (MQ=255)
                                                           cgccgTACTTACATCGGTTCTATGCCGGGTAAACTGTTCCAGAAAATGGCGAAAGTGGGCGTGAAAAACCCGCTGTTCCTGCTCGAGGAGATCGCCacgc                                   >  2:2276034/1‑97 (MQ=255)
                                                             ccgTACTTACATCGGTTCTATGCCGGGTAAACTGTTCCAGAAAATGGCGAAAGTGGGCGTGAAAAACCCGCTGTTCCTGCTCGATGAGATCGACAAAATg                                 >  1:3052827/1‑100 (MQ=255)
                                                               gTACTTACATCGGTTCTATGCCGGGTAAACTGTTCCAGAAAATGGCGAAAGTGGGCGTGAAAAACCCGCTGTTCCTGCTCGATGAGATCGACAAAATGtc                               <  1:2896258/100‑1 (MQ=255)
                                                                tACTTACATCGGTTCTATGCCGGGTAAACTGTTCCAGAAAATGGCGAAAGTGGGCGTGAAAAACCCGCTGTTCCTGCTCGATGAGATCGACAAAATGtct                              <  1:2632821/100‑1 (MQ=255)
                                                                 atctACATCGGTTCTATGCCGGGTAAACTGTTCCAGAAAATGGCGAAAGTGGGCGTGAAAAACCCGCTGTTCCTGCTCGATGAGATCGACAAAATGtctt                             <  2:1707873/97‑1 (MQ=255)
                                                                  cTTACATCGGTTCTATGCCGGGTAAACTGTTCCAGAAAATGGCGAAAGTGGGCGTGAAAAACCCGCTGTTCCTGCTCGATGAGATCGACAAAATGtcttc                            >  1:587683/1‑100 (MQ=255)
                                                                    ttcATCGGTTCTATGCCGGGTAAACTGTTCCAGAAAATGGCGAAAGTGGGCGTGAAAAACCCGCTGTTCCTGCTCGATGAGATCGACAAAATGTCTTCTg                          <  2:2311023/98‑1 (MQ=255)
                                                                    tACATCGGTTCTATGCCGGGTAAACTGTTCCAGAAAATGGCGAAAGTGGGCGTGAAAAACCCGCTGTTCCTGCTCGATGAGATCGACAAAATGTCTTCTg                          >  1:749973/1‑100 (MQ=255)
                                                                     aCATCGGTTCTATGCCGGGTAAACTGTTCCAGAAAATGGCGAAAGTGGGCGTGAAAAACCCGCTGTTCCTGCTCGATGAGATCGACAAAATGTCTTCTGa                         <  2:1420704/100‑1 (MQ=255)
                                                                      cATCGGTTCTATGCCGGGTAAACTGTTCCAGAAAATGGCGAAAGTGGGCGTGAAAAACCCGCTGTTCCTGCTCGATGAGATCGACAAAATGTCTTCTGAc                        >  1:2074289/1‑100 (MQ=255)
                                                                       aTCGGTTCTATGCCGGGTAAACTGTTCCAGAAAATGGCGAAAGTGGGCGTGAAAAACCCGCTGTTCCTGCTCGATGAGATCGACAAAATGTCTTCTGACa                       >  1:827008/1‑100 (MQ=255)
                                                                       aTCGGTTCTATGCCGGGTAAACTGTTCCAGAAAATGGCGAAAGTGGGCGTGAAAAACCCGCTGTTCCTGCTCGATGAGATCGACAAAATGTCTTCTGACa                       <  1:499278/100‑1 (MQ=255)
                                                                          ggTTCTATGCCGGGTAAACTGTTCCAGAAAATGGCGAAAGTGGGCGTGAAAAACCCGCTGTTCCTGCTCGATGAGATCGACAAAATGTCTTCTGACATGc                    <  2:8583/100‑1 (MQ=255)
                                                                          ggTTCTATGCCGGGTAAACTGTTCCAGAAAATGGCGAAAGTGGGCGTGAAAAACCCGCTGTTCCTGCTCGATGAGATCGACAAAATGTCTTCTGACATGc                    >  2:329834/1‑100 (MQ=255)
                                                                           gTTCTATGCCGGGTAAACTGTTCCAGAAAATGGCGAAAGTGGGCGTGAAAAACCCGCTGTTCCTGCTCGATGAGATCGACAAAATGTCTTCTGACATGCg                   >  1:109873/1‑100 (MQ=255)
                                                                               tATGCCGGGTAAACTGTTCCAGAAAATGGCGAGAGTGGGCGTGAAAAACCCGCTGTTCCTGCTCGATGAGATCGACAAAATGTCTTCTGACATGCGTGGc               <  2:1649210/100‑1 (MQ=255)
                                                                               tATGCCGGGTAAACTGTTCCAGAAAATGGCGAAAGTGGGCGTGAAAAACCCGCTGTTCCTGCTCGATGAGATCGACAAAATGTCTTCTGACATGCGTGGc               <  1:324102/100‑1 (MQ=255)
                                                                               tATGCCGGGTAAACTGTTCCAGAAAATGGCGAAAGTGGGCGTGAAAAACCCGCTGTTCCTGCTCGATGAGATCGACAAAATGTCTTCTGACATGCGTGGc               <  2:3353034/100‑1 (MQ=255)
                                                                               tATGCCGGGTAAACTGTTCCAGAAAATGGCGAAAGTGGGCGTGAAAAACCCGCTGTTCCTGCTCGATGAGATCGACAAAATGTCTTCTGACATGCGTGGc               <  2:1297574/100‑1 (MQ=255)
                                                                               tATGCCGGGTAAACTGTTCCAGAAAATGGCGAAAGGGGGCGTGAAAACCCCGCTGTTCCTGCTCGATGAGATCGACAAAATGTCTTCTGACATGCGTGGc               <  2:3675593/100‑1 (MQ=255)
                                                                                aTGCCGGGTAAACTGTTCCAGAAAATGGCGAAAGTGGGCGTGAAAAACCCGCTGTTCCTGCTCGATGAGATCGACAAAATGTCTTCTGACATGCGTGGc               <  2:1418741/99‑1 (MQ=255)
                                                                                aTGCCGGGTAAACTGTTCCAGAAAATGGCGAAAGTGGGCGTGAAAAACCCGCTGTTCCTGCTCGATGAGATCGACAAAATGTCTTCTGACATGCGTGGCg              >  1:911649/1‑100 (MQ=255)
                                                                                   ccGGGTAAACTGTTCCAGAAAATGGCGAAAGTGGGCGTGAAAAACCCGCTGTTCCTGCTCGATGAGATCGACAAAATGTCTTCTGACATGCGTGGCGATc           >  1:51338/1‑100 (MQ=255)
                                                                                     gggTAAACTGTTCCAGAAAATGGCGAAAGTGGGCGTGAAAAACCCGCTGTTCCTGCTCGATGAGATCGACAAAATGTCTTCTGACATGCGTGGCGATCCg         <  1:71180/100‑1 (MQ=255)
                                                                                     gggTAAACTGTTCCAGAAAATGGCGAAAGTGGGCGTGAAAAACCCGCTGTTCCTGCTCGATGAGATCGACAAAATGTCTTCTGACATGCGTGGCGATCCg         <  1:1507339/100‑1 (MQ=255)
                                                                                     gggTAAACTGTTCCAGAAAATGGCGAAAGTGGGCGTGAAAAACCCGCTGTTCCTGCTCGATGAGATCGACAAAATGTCTTCTGACATGCGTGGCGATCCg         >  1:2456056/1‑100 (MQ=255)
                                                                                     gggTAAACTGTTCCAGAAAATGGCGAAAGTGGGCGTGAAAAACCCGCTGTTCCTGCTCGATGAGATCGACAAAATGTCTTCTGACATGCGTGGCGATCCg         <  1:2401235/100‑1 (MQ=255)
                                                                                       gTAAACTGTTCCAGAAAATGGCGAAAGTGGGCGTGAAAAACCCGCTGTTCCTGCTCGATGAGATCGACAAAATGTCTTCTGACATGCGTGGCGATCCGGc       <  2:945402/100‑1 (MQ=255)
                                                                                        tAAACTGTTCCAGAAAATGGCGAAAGTGGGCGTGAAAAACCCGCTGTTCCTGCTCGATGAGATCGACAAAATGTCTTCTGACATGCGTGGCGATCCGGcc      <  2:1932966/100‑1 (MQ=255)
                                                                                        tAAACTGTTCCAGAAAATGGCGAAAGTGGGCGTGAAAAACCCGCTGTTCCTGCTCGATGAGATCGACAAAATGTCTTCTGACATGCGTGGCGATCCGGcc      <  1:2453868/100‑1 (MQ=255)
                                                                                          aaCTGTTCCAGAAAATGGCGAAAGTGGGCGTGAAAAACCCGCTGTTCCTGCTCGATGAGATCGACAAAATGTCTTCTGACATGCGTGGCGATCCGGCctc    <  1:2117932/100‑1 (MQ=255)
                                                                                               |                                                                                                
GTAAATATGTCCGTATGGCGCTGGGCGGCGTGCGTGATGAAGCGGAAATCCGTGGTCACCGCCGTACTTACATCGGTTCTATGCCGGGTAAACTGATCCAGAAAATGGCGAAAGTGGGCGTGAAAAACCCGCTGTTCCTGCTCGATGAGATCGACAAAATGTCTTCTGACATGCGTGGCGATCCGGCCTCTG  >  NC_000913/460011‑460202

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A