Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A8 F232 I1 R1 72 114.8 5816246 94.5% 5496352 99.9

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 460,106 A→T I407F (ATC→TTC)  lon → DNA‑binding ATP‑dependent protease La

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913460,1060AT95.5% 359.1 / 6.2 110I407F (ATC→TTC) lonDNA‑binding ATP‑dependent protease La
Reads supporting (aligned to +/- strand):  ref base A (1/4);  new base T (52/53);  total (53/57)
Fisher's exact test for biased strand distribution p-value = 3.65e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.83e-01

Only 100 of 110 total aligned reads displayed.

GCGTAAATATGTCCGTATGGCGCTGGGCGGCGTGCGTGATGAAGCGGAAATCCGTGGTCACCGCCGTACTTACATCGGTTCTATGCCGGGTAAACTGATCCAGAAAATGGCGAAAGTGGGCGTGAAAAACCCGCTGTTCCTGCTCGATGAGATCGACAAAATGTCTTCTGACATGCGTGGCGATCCGGCCTC  >  NC_000913/460009‑460200
                                                                                                 |                                                                                              
gCGTAAATATGTCCGTATGGCGCTGGGCGGCGTGCGTGATGAAGCGGAAATCCGTGGTCACCGCCGTACTTACATCGGTTCTATGCCGGGTAAACTGTTc                                                                                              <  2:1630103/100‑1 (MQ=255)
gCGTAAATATGTCCGTATGGCGCTGGGCGGCGTGCGTGATGAAGCGGAAATCCGTGGTCACCGCCGTACTTACATCGGTTCTATGCCGGGTAAACTGTTc                                                                                              <  2:1817238/100‑1 (MQ=255)
gCGTAAATATGTCCGTATGGCGCTGGGCGGCGTGCGTGATGAAGCGGAAATCCGTGGTCACCGCCGTACTTACATCGGTTCTATGCCGGGTAAACTGTTc                                                                                              <  1:2860744/100‑1 (MQ=255)
gCGTAAATATGTCCGTATGGCGCTGGGCGGCGTGCGTGATGAAGCGGAAATCCGTGGTCACCGCCGTACTTACATCGGTTCTATGCCGGGTAAACTGTTc                                                                                              <  1:2860681/100‑1 (MQ=255)
 cGTAAATATGTCCGTATGGCGCTGGGCGGCGTGCGTGATGAAGCGGAAATCCGTGGTCACCGCCGTACTTACATCGGTTCTATGCCGGGTAAACTGTTcc                                                                                             >  2:2847555/1‑100 (MQ=255)
  gTAAATATGTCCGTATGGCGCTGGGCGGCGTGCGTGATGAAGCGGAAATCCGTGGTCACCGCCGTACTTACATCGGTTCTATGCCGGGTAAACTGTTCCa                                                                                            >  1:2684267/1‑100 (MQ=255)
  gTAAATATGTCCGTATGGCGCTGGGCGGCGTGCGTGATGAAGCGGAAATCCGTGGTCACCGCCGTACTTACATCGGTTCTATGCCGGGTAAACTGTTCCa                                                                                            <  1:2585871/100‑1 (MQ=255)
   tAAATATGTCCGTATGGCGCTGGGCGGCGTGCGTGATGAAGCGGAAATCCGTGGTCACCGCCGTACTTACATCGGTTCTATGCCGGGTAAACTGTTCCa                                                                                            <  1:2459061/99‑1 (MQ=255)
    aaaTATGTCCGTATGGCGCTGGGCGGCGTGCGTGATGAAGCGGAAATCCGTGGTCACCGCCGTACTTACATCGGTTCTATGCCGGGTAAACTGTTCCAGa                                                                                          >  1:2148468/1‑100 (MQ=255)
      atatGTCCGTATGGCGCTGGGCGGCGTGCGTGATGAAGCGGAAATCCGTGGTCACCGCCGTACTTACATCGGTTCTATGCCGGGTAAACTGTTCCAGaaa                                                                                        <  1:837085/100‑1 (MQ=255)
        atGTCCGTATGGCGCTGGGCGGCGTGCGTGATGAAGCGAAAATCCGTGGTCACCGCCGTACTTACATCGGTTCTATGCCGGGTAAACTGTTCCAGAAAAt                                                                                      >  1:226667/1‑100 (MQ=255)
          gTCCGTATGGCGCTGGGCGGCGTGCGTGATGAAGCGGAAATCCGTGGTCACCGCCGTACTTACATCGGTTCTATGCCGGGTAAACTGTTCCAGAAAATgg                                                                                    >  2:500884/1‑100 (MQ=255)
          gTCCGTATGGCGCTGGGCGGCGAGCGTGATGAAGCGGAAATCCGTGGTCACCGCCGTACTTACATCGGTTCTATGCCGGGTAAACTGTTCCAGAAAATgg                                                                                    <  2:2210756/100‑1 (MQ=255)
           tCCGTATGGCGCTGGGCGGCGTGCGTGATGAAGCGGAAATCCGTGGTCACCGCCGTACTTACATCGGTTCTATGCCGGGTAAACTGTTCCAGAAAATGGc                                                                                   <  2:902370/100‑1 (MQ=255)
           tCCGTATGGCGCTGGGCGGCGTGCGTGATGAAGCGGAAATCCGTGGTCACCGCCGTACTTACATCGGTTCTATGCCGGGTAAACTGTTCCAGAAAATGGc                                                                                   <  1:199069/100‑1 (MQ=255)
           tCCGTATGGCGCTGGGCGGCGTGCGTGATGAAGCGGAAATCCGTGGTCACCGCCGTACTTACATCGGTTCTATGCCGGGTAAACTGTTCCAGAAAATGGc                                                                                   <  2:49392/100‑1 (MQ=255)
            ccGTATGGCGCTGGGCGGCGTGCGTGATGAAGCGGAAATCCGTGGTCACCGCCGTACTTACATCGGTTCTATGCCGGGTAAACTGTTCCAGAAAATGGCg                                                                                  >  2:697663/1‑100 (MQ=255)
              gTATGGCGCTGGGCGGCGTGCGTGATGAAGCGGAAATCCGTGGTCACCGCCGTACTTACATCGGTTCTATGCCGGGTAAACTGTTCCAGAAAATGGCGaa                                                                                >  2:697091/1‑100 (MQ=255)
               tATGGCGCTGGGCGGCGTGCGTGATGAAGCGGAAATCCGTGGTCACCGCCGTACTTACATCGGTTCTATGCCGGGTAAACTGTTCCAGAAAATGGCGaaa                                                                               >  1:1601516/1‑100 (MQ=255)
               tATGGCGCTGGGCGGCGTGCGTGATGAAGCGGAAATCCGTGGTCACCGCCGTACTTACATCGGTTCTATGCCGGGTAAACTGTTCCAGAAAATGGCGaaa                                                                               <  2:2164527/100‑1 (MQ=255)
                  ggCGCTGGGCGGCGTGCGTGATGAAGCGGAAATCCGTGGTCACCGCCGTACTTACATCGGTTCTATGCCGGGTAAACTGTTCCAGAAAATGGCGAAAGTg                                                                            >  1:1173656/1‑100 (MQ=255)
                  ggCGCTGGGCGGCGTGCGTGATGAAGCGGAAATCCGTGGTCACCGCCGTACTTACATCGGTTCTATGCCGGGTAAACTGTTCCAGAAAATGGCGAAAGTg                                                                            >  2:1382402/1‑100 (MQ=255)
                  ggCGCTGGGCGGCGTGCGTGATGAAGCGGAAATCCGTGGTCACCGCCGTACTTACATCGGTTCTATGCCGGGTAAACTGTTCCAGAAAATGGCGAAAGTg                                                                            <  1:2070592/100‑1 (MQ=255)
                  ggCGCTGGGCGGCGTGCGTGATGAAGCGGAAATCCGTGGTCACCGCCGTACTTACATCGGTTCTATGCCGGGTAAACTGTTCCAGAAAATGGCGAAAGTg                                                                            >  1:2663963/1‑100 (MQ=255)
                    cgcTGGGCGGCGTGCGTGATGAAGCGGAAATCCGTGGTCACCGCCGTACTTACATCGGTTCTATGCCGGGTAAACTGTTCCAGAAAATGGCGAAAGTggg                                                                          >  2:2104338/1‑100 (MQ=255)
                     gcTGGGCGGCGTGCGTGATGAAGCGGAAATCCGTGGTCACCGCCGTACTTACATCGGTTCTATGCCGGGTAAACTGATCCAGAAAATGGCGAAAGTGGGc                                                                         <  2:485921/100‑1 (MQ=255)
                      cTGGGCGGCGTGCGTGATGAAGCGGAAATCCGTGGTCACCGCCGTACTTACATCGGTTCTATGCCGGGTAAACTGTTCCAGAAAATGGCGAAAGTGGGCg                                                                        <  2:2163001/100‑1 (MQ=255)
                        gggCGGCGTGCGTGATGAAGCGGAAATCCGTGGTCACCGCCGTACTTACATCGGTTCTATGCCGGGTAAACTGTTCCAGAAAATGGCGAAAGTGGGCGTg                                                                      >  2:1281369/1‑100 (MQ=255)
                        gggCGGCGTGCGTGATGAAGCGGAAATCCGTGGTCACCGCCGTACTTACATCGGTTCTATGCCGGGTAAACTGTTCCAGAAAATGGCGAAAGTGGGCGTg                                                                      <  2:2092903/100‑1 (MQ=255)
                        gggCGGCGTGCGTGATGAAGCGGAAATCCGTGGTCACCGCCGTACTTACATCGGTTCTATGCCGGGTAAACTGTTCCAGAAAATGGCGAAAGTGGGCGTg                                                                      <  1:1561785/100‑1 (MQ=255)
                          gcggcgTGCGTGATGAAGCGGAAATCCGTGGTCACCGCCGTACTTACATCGGTTCTATGCCGGGTAAACTGTTCCAGAAAATGGCGAAAGTGGGCGTGaa                                                                    >  1:1414648/1‑100 (MQ=255)
                          gcggcgTGCGTGATGAAGCGGAAATCCGTGGTCACCGCCGTACTTACATCGGTTCTATGCCGGGTAAACTGTTCCAGAAAATGGCGAAAGTGGGCGTGaa                                                                    >  1:640820/1‑100 (MQ=255)
                           cggcgTGCGTGATGAAGCGGAAATCCGTGGTCACCGCCGTACTTACATCGGTTCTATGCCGGGTAAACTGTTCCAGAAAATGGCGAAAGTGGGCGTGaaa                                                                   >  1:1157612/1‑100 (MQ=255)
                             gcgtgcgtGATGAAGCGGAAATCCGTGGTCACCGCCGTACTTACATCGGTTCTATGCCGGGTAAACTGTTCCAGAAAATGGCGAAAGTGGGCGTGaaaaa                                                                 <  2:578746/100‑1 (MQ=255)
                                 gcgtgATGAAGCGGAAATCCGTGGTCACCGCCGTACTTACATCGGTTCTATGCCGGGTAAACTGTTCCAGAAAATGGCGAAAGTGGGCGTGAAAAACCCg                                                             <  2:2247934/100‑1 (MQ=255)
                                  cgtgATGAAGCGGAAATCCGTGGTCACCGCCGTACTTACATCGGTTCTATGCCGGGTAAACTGTTCCAGAAAATGGCGAAAGTGGGCGTGAAAAACCCGc                                                            >  2:1662502/1‑100 (MQ=255)
                                  cgtgATGAAGCGGAAATCCGTGGTCACCGCCGTACTTACATCGGTTCTATGCCGGGTAAACTGTTCCAGAAAATGGCGAAAGTGGGCGTGAAAAACCCGc                                                            <  2:2530687/100‑1 (MQ=255)
                                   ctgatgaAGCGGAAATCCGTGGTCACCGCCGTACTTACATCGGTTCTATGCCGGGTAAACTGTTCCAGAAAATGGCGAAAGTGGGCGTGAAAAACCCGCt                                                           <  2:2608382/99‑1 (MQ=255)
                                    tgatgaAGCGGAAATCCGTGGTCACCGCCGTACTTACATCGGTTCTATGCCGGGTAAACTGTTCCAGAAAATGGCGAAAGTGGGCGTGAAAAACCCGCTg                                                          >  2:635029/1‑100 (MQ=255)
                                    tgatgaAGCGGAAATCCGTGGTCACCGCCGTACTTACATCGGTTCTATGCCGGGTAAACTGTTCCAGAAAATGGCGAAAGTGGGCGTGAAAAACCCGCTg                                                          >  1:2228073/1‑100 (MQ=255)
                                    tgatgaAGCGGAAATCCGTGGTCACCGCCGTACTTACATCGGTTCTATGCCGGGTAAACTGTTCCAGAAAATGGCGAAAGTGGGCGTGAAAAACCCGCTg                                                          <  2:1456974/100‑1 (MQ=255)
                                    tgatgaAGCGGAAATCCGTGGTCACCGCCGTACTTACATCGGTTCTATGCCGGGTAAACTGATCCAGAAAATGGCGAAAGTGGGCGTGAAAAACCCGCTg                                                          <  1:2269487/100‑1 (MQ=255)
                                     gatgaAGCGGAAATCCGTGGTCACCGCCGTACTTACATCGGTTCTATGCCGGGTAAACTGTTCCAGAAAATGGCGAAAGTGGGCGTGAAAAACCCGCtag                                                         >  1:2608349/1‑98 (MQ=255)
                                      atgaAGCGGAAATCCGTGGTCACCGCCGTACTTACATCGGTTCTATGCCGGGTAAACTGTTCCAGAAAATGGCGAAAGTGGGCGTGAAAAACCCGCTGtt                                                        >  2:1008862/1‑100 (MQ=255)
                                       tgaAGCGGAAATCCGTGGTCACCGCCGTACTTACATCGGTTCTATGCCGGGTAAACTGTTCCAGAAAATGGCGAAAGTGGGCGTGAAAAACCCGCTGTTc                                                       >  1:2694872/1‑100 (MQ=255)
                                       tgaAGCGGAAATCCGTGGTCACCGCCGTACTTACATCGGTTCTATGCCGGGTAAACTGTTCCAGAAAATGGCGAAAGTGGGCGTGAAAAACCCGCTGTTc                                                       >  1:413712/1‑100 (MQ=255)
                                        gaAGCGGGAATCCGTGGTCACCGCCGTACGTACATCGGTGCTATGCCGGGTAAACTGTGCCAGAAAATGGCGCAAGTAGGTGTGAAAATCCCGCt                                                           <  1:2496065/95‑1 (MQ=255)
                                         aaGCGTAAATCCGTGGTCACCGCCGTACTTACATCGGTTCTATGCCGGGTAAACTGTTCCAGAAAATGGCGAAAGTGGGCGTGAAAAACCCGCTGTTCCt                                                     <  2:2423234/100‑1 (MQ=255)
                                            cGGAAATCCGTGGTCACCGCCGTACTTACATCGGTTCTATGCCGGGTAAACTGTTCCAGAAAATGGCGAAAGTGGGCGTGAAAAACCCGCTGTTCCTGCt                                                  <  2:2088662/100‑1 (MQ=255)
                                            cGGAAATCCGTGGTCACCGCCGTACTTACATCGGTTCTATGCCGGGTAAACTGTTCCAGAAAATGGCGAAAGTGGGCGTGAAAAACCCGCTGTTCCTGCt                                                  <  1:1071790/100‑1 (MQ=255)
                                            cGGAAATCCGTGGTCACCGCCGTACTTACATCGGTTCTATGCCGGGTAAACTGTTCCAGAAAATGGCGAAAGTGGGCGTGAAAAACCCGCTGTTCCTGCt                                                  <  2:1295489/100‑1 (MQ=255)
                                             ggAAATCCGTGGTCACCGCCGTACTTACATCGGTTCTATGCCGGGTAAACTGTTCCAGAAAATGGCGAAAGTGGGCGTGAAAAACCCGCTGTTCCTGCTc                                                 >  1:2149033/1‑100 (MQ=255)
                                             ggAAATCCGTGGTCACCGCCGTACTTACATCGGTTCTATGCCGGGTAAACTGTTCCAGAAAATGGCGAAAGTGGGCGTGAAAAACCCGCTGTTCCTGCTc                                                 >  1:2531198/1‑100 (MQ=255)
                                              gAAATCCGTGGTCACCGCCGTACTTACATCGGTTCTATGCCGGGTAAACTGTTCCGGAAAATGGCGAAAGTGGGCGTGAAAAACCCGCTGTTCCTGCTCg                                                <  2:2672477/100‑1 (MQ=255)
                                                 aTCCGTGGTCACCGCCGTACTTACATCGGTTCTATGCCGGGTAAACTGTTCCAGAAAATGGCGAAAGTGGGCGTGAAAAACCCGCTGTTCCTGCTCGATg                                             <  2:2033544/100‑1 (MQ=255)
                                                 aTCCGTGGTCACCGCCGTACTTACATCGGTTCTATGCCGGGTAAACTGTTCCAGAAAATGGCGAAAGTGGGCGTGAAAAACCCGCTGTTCCTGCTCGATg                                             <  2:2684303/100‑1 (MQ=255)
                                                 aTCCGTGGTCACAGCCGTACTTACATCGGTTCTATGCCGGGTAAACTGTTCCAGAAAATGGCGAAAGTGGGCGTGAAAAACCCGCTGTTCCTGCTCGATg                                             >  2:1907944/1‑100 (MQ=255)
                                                   ccGTGGTCACCGAGGTACTTACATCGGTTCTATGCCGGGTAAACTGTGCCACACAATGGCAAAAGTGGGCGTGAAAAACCCGCTGTTGCTACTCGATgag                                           >  2:2894050/1‑100 (MQ=255)
                                                    cGTGGTCACCGCCGTACTTACATCGGTTCTATGCCGGGTAAACTGTTCCAGAAAATGGCGAAAGTGGGCGTGAAAAACCCGCTGTTCCTGCTCGATgaga                                          >  2:1101105/1‑100 (MQ=255)
                                                     gTGGTCACCGCCGTACTTACATCGGTTCTATGCCGGGTAAACTGTTCCAGAAAATGGCGAAAGTGGGCGTGAAAAACCCGCTGTTCCTGCTCGATGAGAt                                         <  2:1269046/100‑1 (MQ=255)
                                                      tGGTCACCGCCGTACTTACATCGGTTCTATGCCGGGTAAACTGTTCCAGAAAATGGCGAAAGTGGGCGTGAAAAACCCGCTGTTCCTGCTCGATGAGATc                                        >  1:658093/1‑100 (MQ=255)
                                                      tGGTCACCGCCGTACTTACATCGGTTCTATGCCGGGTAAACTGTTCCAGAAAATGGCGAAAGTGGGCGTGAAAAACCCGCTGTTCCTGCTCGATGAGATc                                        >  1:657968/1‑100 (MQ=255)
                                                         tCACCGCCGTACTTACATCGGTTCTATGCCGGGTAAACTGTTCCAGAAAATGGCGAAAGTGGGCGTGAAAAACCCGCTGTTCCTGCTCGATGAGATCGAc                                     >  2:1452976/1‑100 (MQ=255)
                                                          cACCGCCGTACTTACATCGGTTCTATGCCGGGTAAACTGTTCCAGAAAATGGCGAAAGTGGGCGTGAAAAACCCGCTGTTCCTGCTCGATGAGATCGACa                                    >  2:836731/1‑100 (MQ=255)
                                                          cACCGCCGTACTTACATCGGTTCTATGCCGGGTAAACTGTTCCAGAAAATGGCGAAAGTGGGCGTGAAAAACCCGCTGTTCCTGCTCGATGAGATCGACa                                    >  2:1743067/1‑100 (MQ=255)
                                                            ccgccgTACTTACATCGGTTCTATGCCGGGTAAACTGTTCCAGAAAATGGCGAAAGTGGGCGTGAAAAACCCGCTGTTCCTGCTCGATGAGATCGACaaa                                  >  1:41421/1‑100 (MQ=255)
                                                              gccgTACTTACATCGGTTCTATGCCGGGTAAACTGTTCCAGAAAATGGCGAAAGTGGGCGTGAAAAACCCGCTGTTCCTGCTCGATGAGATCGACAAAAt                                <  2:226670/100‑1 (MQ=255)
                                                               ccgTACTTACATCGGTTCTATGCCGGGTAAACTGTTCCAGAAAATGGCGAAAGTGGGCGTGAAAAACCCGCTGTTCCTGCTCGATGAGATCGACAAAATg                               >  1:2680995/1‑100 (MQ=255)
                                                                 gTACTTACATCGGTTCTATGCCGGGTAAACTGTTCCAGAAAATGGCGAAAGTGGGCGTGAAAAACCCGCTGTTCCTGCTCGATGAGATCGACAAAATGtc                             <  2:2663999/100‑1 (MQ=255)
                                                                 gTACTTACATCGGTTCTATGCCGGGTAAACTGATCCAGAAAATGGCGAAAGTGGGCGTGAAAAACCCGCTGTTCCTGCTCGATGAGATCGACAAAATGtc                             >  1:1437973/1‑100 (MQ=255)
                                                                    cTTACATCGGTTCTATGCCGGGTAAACTGTTCCAGAAAATGGCGAAAGTGGGCGTGAAAAACCCGCTGTTCCTGCTCGATGAGATCGACAAAATGtcttc                          >  2:109840/1‑100 (MQ=255)
                                                                    cTTACATCGGTTCTATGCCGGGTAAACTGTTCCAGAAAATGGCGAAAGTGGGCGTGAAAAACCCGCTGTTCCTGCTCGATGAGATCGACAAAATGtcttc                          >  1:949377/1‑100 (MQ=255)
                                                                     ccgatctggTTCTATGCCGGGTAAACTGTTCCAGAAAATGGCGAAAGTGGGCGTGAAAAACCCGCTGTTCCTGCTCGATGAGATCGACAAAATGtcttct                         <  1:1134602/93‑1 (MQ=255)
                                                                      tACATCGGTTCTATGCCGGGTAAACTGTTTCAGAAAATGGCGAAAGTGGGCGTGAAAAACCCGCTGCTCCTGCTCGATGAGATCGACAAAATGTCTTCTg                        <  1:1743041/100‑1 (MQ=255)
                                                                      tACATCGGTTCTATGCCGGGTAAACTGTTCCAGAAAATGGCGAAAGTGGGCGTGAAAAACCCGCTGTTCCTGCTCGATGAGATCGACAAAATGTCTTCTg                        >  2:1004678/1‑100 (MQ=255)
                                                                      tACATCGGTTCTATGCCGGGTAAACTGTTCCAGAAAATGGCGAAAGTGGGCGTGAAAAACCCGCTGTTCCTGCTCGATGAGATCGACAAAATGTCTTCTg                        <  1:25224/100‑1 (MQ=255)
                                                                       aCATCGGTTCTATGCCGGGTAAACTGTTCCAGAAAATGGCGAAAGTGGGCGTGAAAAACCCGCTGTTCCTGCTCGATGAGATCGACAAAATGTCTTCTGa                       <  1:172922/100‑1 (MQ=255)
                                                                        cATCGGTTCTATGCCGGGTAAACTGTTCCAGAAAATGGCGAAAGTGGGCGTGAAAAACCCGCTGTTCCTGCTCGATGAGATCGACAAAATGTCTTCTGAc                      <  1:1008852/100‑1 (MQ=255)
                                                                        cATCGGTTCTATGCCGGGTAAACTGTTCCAGAAAATGGCGAAAGTGGGCGTGAAAAACCCGCTGTTCCTGCTCGATGAGATCGACAAAATGTCTTCTGAc                      >  2:2425801/1‑100 (MQ=255)
                                                                        cATCGGTTCTATGCCGGGTAAACTGTTCCAGAAAATGGCGAAAGTGGGCGTGAAAAACCCGCTGTTCCTGCTCGATGAGATCGACAAAATGTCTTCTGAc                      >  1:1076264/1‑100 (MQ=255)
                                                                        cATCGGTTCTATGCCGGGTAAACTGTTCCAGAAAATGGCGAAAGTGGGCGTGAAAAACCCGCTGTTCCTGCTCGATGAGATCGACAAAATGTCTTCTGAc                      >  2:127346/1‑100 (MQ=255)
                                                                         aTCGGTTCTATGCCGGGTAACCTGTTCCAGAAAATGGCGAAAGTGGGCGTGAAAAACCCGCTGTTCCTGCTCGATGAGATCGACAAAATGTCTTCTGACa                     >  1:614564/1‑100 (MQ=255)
                                                                          tCGGTTCTATGCCGGGTAAACTGTTCCAGAAAATGGCGAAAGTGGGCGTGAAAAACCCGCTGTTCCTGCTCGATGAGATCGACAAAATGTCTTCTGACAt                    >  2:2777011/1‑100 (MQ=255)
                                                                           tccgatcttGCCGGGTAAACTGTTCCAGAAAATGGCGAAAGTGGGCGTGAAAAACCCGCTGTTCCTGCTCGATGAGATCGACAAAATGTCTTCTGACATg                   <  2:1480835/92‑1 (MQ=255)
                                                                            ggTTCTATGCCGGGTAAACTGTTCCAGAAAATGGCGAAAGTGGGCGTGAAAAACCCGCTGTTCCTGCTCGATGAGATCGACAAAATGtcttctagatcgg                  >  2:1134620/1‑93 (MQ=255)
                                                                               tCTATGCCGGGTAAACTGTTCCAGAAAATGGCGAAAGTGGGCGTGAAAAACCCGCTGTTCCTGCTCGATGAGATCGACAAAATGTCTTCTGACATGCGTg               <  1:161987/100‑1 (MQ=255)
                                                                                cTATGCCGGGTAAACTGTTCCAGAAAATGGCGAAAGTGGGCGTGAAAAACCCGCTGTTCCTGCTCGATGAGATCGACAAAATGTCTTCTGACATGCGTgg              <  1:1781087/100‑1 (MQ=255)
                                                                                cTATGCCGGGTAAACTGTTCCAGAAAATGGCGAAAGTGGGCGTGAAAAACCCGCTGTTCCTGCTCGATGAGATCGACAAAATGTCTTCTGACATGCGTgg              <  1:2695442/100‑1 (MQ=255)
                                                                                 tATGCCGGGTAAACTGTTCCAGAAACTGGCGAAAGTGGGCGTGAAAAACCCGCTGTTCCTGCTCGATGAAATCGACAAAATATCTTCTGACATGCGTgg              >  1:954529/1‑99 (MQ=255)
                                                                                 tATGCCGGGTAAACTGTTCCAGAAAATGGCGAAAGTGGGCGTGAAAAACCCGCTGTTCCTGCTCGATGAGATCGACAAAATGTCTTCTGACATGCGTGGc             <  1:1246500/100‑1 (MQ=255)
                                                                                 tATGCCGGGTAAACTGTTCCAGAAAATGGCGAAAGTGGGCGTGAAAAACCCGCTGTTCCTGCTCGATGAGATCGACAAAATGTCTTCTGACATGCGTGGc             >  2:1277379/1‑100 (MQ=255)
                                                                                 tATGCCGGGTAAACTGTTCCAGAAAATGGCGAAAGTGGGCGTGAAAAACCCGCTGTTCCTGCTCGATGAGATCGACAAAATGTCTTCTGACATGCGTGGc             <  1:635023/100‑1 (MQ=255)
                                                                                  aTGCCGGGTAAACTGTTCCAGAAAATGGCGAAAGTGGGCGTGAAAAACCCGCTGTTCCTGCTCGATGAGATCGACAAAATGTCTTCTGACATGCGTGGCg            >  1:1566967/1‑100 (MQ=255)
                                                                                   tGCCGGGTAAACTGTTCCAGAAAATGGCGAAAGTGGGCGTGAAAAACCCGCTGTTCCTGCTCGATGAGATCGACAAAATGTCTTCTGACATgagatcgga           >  1:1480811/1‑92 (MQ=255)
                                                                                    gCCGGGTAAACTGATCCAGAAAATGGCGAAAGTGGGCGTGAAAAACCCGCTGTTCCTGCTCGATGAGATCGACAAAATGTCTTCTGACATGCGTGGCGAt          <  2:787767/100‑1 (MQ=255)
                                                                                       gggTAAACTGTTCCAGAAAATGGCGAAAGTGGGCGTGAAAAACCCGCTGTTCCTGCTCGATGAGATCGACAAAATGTCTTCTGACATGCGTGGCGATCCg       <  2:1527999/100‑1 (MQ=255)
                                                                                         gTAAACTGATCCAGAAAATGGCGAAAGTGGGCGTGAAAAACCCGCTGTTCCTGCTCGATGAGATCGACAAAATGTCTTCTGACATGCGTGGCGATCCGGc     <  2:1437992/100‑1 (MQ=255)
                                                                                          tAAACTGTTCCAGAAAATGGCGAAAGTGGGCGTGAAAAACCCGCTGTTCCTGCTCGATGAGATCGACAAAATGTCTTCTGACATGCGTGGCGATCCGGcc    <  2:1646345/100‑1 (MQ=255)
                                                                                           aaaCTGTTCCAGAAAATGGCGAAAGTGGGCGTGAAAAACCCGCTGTTCCTGCTCGATGAGATCGACAAAATGTCTTCTGACATGCGTGGCGATCCGGcca   >  1:1646319/1‑99 (MQ=255)
                                                                                            aaCTGTTCCAGAAAATGGCGAAAGTGGGCGTGAAAAACCCGCTGTTCCTGCTCGATGAGATCGACAAAATGTCTTCTGACATGCGTGGCGATCCGGCctc  >  2:1198795/1‑100 (MQ=255)
                                                                                                 |                                                                                              
GCGTAAATATGTCCGTATGGCGCTGGGCGGCGTGCGTGATGAAGCGGAAATCCGTGGTCACCGCCGTACTTACATCGGTTCTATGCCGGGTAAACTGATCCAGAAAATGGCGAAAGTGGGCGTGAAAAACCCGCTGTTCCTGCTCGATGAGATCGACAAAATGTCTTCTGACATGCGTGGCGATCCGGCCTC  >  NC_000913/460009‑460200

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A