Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A8 F232 I0 R1 243 156.3 7646460 93.8% 7172379 99.8

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 4,465,236 C→T 100% P315P (CCG→CCA)  treB ← trehalose‑specific PTS enzyme: IIB and IIC component

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,465,2360CT98.7% 562.7 / ‑3.4 172P315P (CCG→CCA) treBtrehalose‑specific PTS enzyme: IIB and IIC component
Reads supporting (aligned to +/- strand):  ref base C (3/0);  new base T (94/75);  total (97/75)
Fisher's exact test for biased strand distribution p-value = 2.58e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 172 total aligned reads displayed.

ACAGCGCAATCAGCGGCCACACTGGCGTACCACCCATGCTTTGAATCATCTGCAAATCAATAGCAAGCGTGGTCTGGTGTACACCGGTGATCACCAGCGGGGCGTACAGGAAGCCAAACAATGCTGCGCCAATCGGAGCAAAGCTGCCGGTCATCAGGTGACGTACCGCAAAGGCAACGCCATCGCCAATC  >  NC_000913/4465139‑4465329
                                                                                                 |                                                                                             
  aGCGCAATCAGCGGCCACACTGGCGTACCACCCATGCTTTGAATCATCTGCAAATCAATAGCAAGCGTGGTCTGGTGTACACCGGTGATCACCAGTgggg                                                                                           <  1:3166709/100‑1 (MQ=255)
  aGCGCAATCAGCGGCCACACTGGCGTACCACCCATGCTTTGAATCATCTGCAAATCAATAGCAAGCGTGGTCTGGTGTACACCGGTGATCACCAGTgggg                                                                                           <  2:2800167/100‑1 (MQ=255)
   gcgcAATCAGCGGCCACACTGGCGTACCACCCATGCTTTGAATCATCTGCAAATCAATAGCAAGCGTGGTCTGGTGTACACCGGTGATCACCAGTGGGGc                                                                                          >  2:1351248/1‑100 (MQ=255)
    cgcAATCAGCGGCCACACTGGCGTACCACCCATGCTTTGAATCATCTGCAAATCAATAGCAAGCGTGGTCTGGTGTACACCGGTGATCACCAGTGGGGCg                                                                                         <  2:1889819/100‑1 (MQ=255)
     gcAATCAGCGGCCACACTGGCGTACCACCCATGCTTTGAATCATCTGCAAATCAATAGCAAGCGTGGTCTGGTGTACACCGGTGATCACCAGTGGGGCGt                                                                                        >  2:21322/1‑100 (MQ=255)
      cgaTCAGCGGCCACACTGGCGTACCACCCATGCTTTGAATCAACTGCAAATCAATAGCAAGCGTGGTCTGGTGTACACCGGTGATCACCAGTGGGGCGTa                                                                                       >  1:2582118/3‑100 (MQ=255)
      cAATCAGCGGCCACACTGGCGTACCACCCATGCTTTGAATCATCTGCAAATCAATAGCAAGCGTGGTCTGGTGTACACCGGTGATCACCAGTGGGGCGTa                                                                                       >  2:836083/1‑100 (MQ=255)
       aaTCAGCGGCCACACTGGCGTACCACCCATGCTTTGAATCATCTGCAAATCAATAGCAAGCGTGGTCTGGTGTACACCGGTGATCACCAGTGGGGCGTAc                                                                                      <  1:236351/100‑1 (MQ=255)
          cAGCGGCCACACTGGCGTACCACCCATGCTTTGAATCATCTGCAAATCAATAGCAAGCGTGGTCTGGTGTACACCGGTGATCACCAGTGGGGCGTACAgg                                                                                   <  1:1288419/100‑1 (MQ=255)
          cAGCGGCCACACTGGCGTACCACCCATGCTTTGAATCATCTGCAAATCAATAGCAAGCGTGGTCTGGTGTACACCGGTGATCACCAGTGGGGCGTACAgg                                                                                   <  1:3518337/100‑1 (MQ=255)
          cAGCGGCCACACTGGCGTACCACCCATGCTTTGAATCATCTGCAAATCAATAGCAAGCGTGGTCTGGTGTACACCGGTGATCACCAGTGGGGCGTACAgg                                                                                   <  2:1656685/100‑1 (MQ=255)
           aGCGGCCACACTGGCGTACCACCCATGCTTTGAATCATCTGCAAATCAATAGCAAGCGTGGTCTGGTGTACACCGGTGATCACCAGTGGGGCGTACAGGa                                                                                  >  1:44859/1‑100 (MQ=255)
           aGCGGCCACACTGGCGTACCACCCATGCTTTGAATCATCTGCAAATCAATAGCAAGCGTGGTCTGGTGTACACCGGTGATCACCAGTGGGGCGTACAGGa                                                                                  >  2:3784929/1‑100 (MQ=255)
           aGCGGCCACACTGGCGTACCACCCATGCTTTGAATCATCTGCAAATCAATAGCAAGCGTGGTCTGGTGTACACCGGTGATCACCAGTGGGGCGTACAGGa                                                                                  <  2:1355146/100‑1 (MQ=255)
           aGCGGCCACACTGGCGTACCACCCATGCTTTGAATCATCTGCAAATCAATAGCAAGCGTGGTCTGGTGTACACCGGTGATCACCAGTGGGGCGTACAGGa                                                                                  >  1:2316849/1‑100 (MQ=255)
            gCGGCCACACTGGCGTACCACCCATGCTTTGAATCATCTGCAAATCAATAGCAAGCGTGGTCTGGTGTACACCGGTGATCACCAGTGGGGCGTACAGGaa                                                                                 <  1:789652/100‑1 (MQ=255)
              ggCCACACTGGCGTACCACCCATGCTTTGAATCATCTGCAAATCAATAGCAAGCGTGGTCTGGTGTACACCGGTGATCACCAGTGGGGCGTACAGGAAGc                                                                               >  1:1711860/1‑100 (MQ=255)
               gCCACACTGGCGTACCACCCATGCTTTGAATCATCTGCAAATCAATAGCAAGCGTGGTCTGGTGTACACCGGTGATCACCAGTGGGGCGTACAGGAAGcc                                                                              <  2:1158869/100‑1 (MQ=255)
               gCCACACTGGCGTACCACCCATGCTTTGAATCATCCGCAAATCAATAGCAAGCGTGGTCTGGTGTACACCGGTGATCACCAGTGGGGCGTACAGGAAGcc                                                                              >  1:430662/1‑100 (MQ=255)
                   cacTGGCGTACCACCCATGCTTTGAATCATCTGCAAATCAATAGCAAGCGTGGTCTGGTGTACACCGGTGATCACCAGTGGGGCGTACAGGAAGCCAAAc                                                                          <  2:1848829/100‑1 (MQ=255)
                   cacTGGCGTACCACACATGCTTTGAATCATCTGCAAATCAATAGCAAGCGTGGTCTGGTGTACACCGGTGATCACCAGTGGGGCGTACAGGAAGCCAAAc                                                                          >  2:262779/1‑100 (MQ=255)
                     cTGGCGTACCACCCATGCTTTGAATCATCTGCAAATCAATAGCAAGCGTGGTCTGGTGTACACCGGTGATCACCAGTGGGGCGTACAGGAAGCCAAACaa                                                                        >  1:2914845/1‑100 (MQ=255)
                     cTGGCGTACCACCCATGCTTTGAAGCATCTGCAAAGCAATAGCAAGCGTGGTCTGGTGTACACCGGTGATCACCAGTGGGGCGTACAGGAAGCCAAACaa                                                                        <  2:1798008/100‑1 (MQ=255)
                        gCGTACCACCCATGCTTTGAATCATCTGCAAATCAATAGCAAGCGTGGTCTGGTGTACACCGGTGATCACCAGTGGGGCGTACAGGAAGCCACACAAtgc                                                                     >  2:2612143/1‑100 (MQ=255)
                        gCGTACCACCCATGCTTTGAATCATCTGCAAATCAATAGCAAGCGTGGTCTGGTGTACACCGGTGATCACCAGTGGGGCGTACAGGAAGCCAAACAAtgc                                                                     <  2:2316834/100‑1 (MQ=255)
                        gCGTACCACCCATGCTTTGAATCATCTGCAAATCAATAGCAAGCGTGGTCTGGTGTACACCGGTGATCACCAGTGGGGCGTACAGGAAGCCAAACAAtgc                                                                     <  2:3541868/100‑1 (MQ=255)
                         cGTACCACCCATGCTTTGAAACATCTGCAAATCAATAGCAAGCGTGGTCTGGTGTACACCGGTGATCACCAGTGAGGCGTACAGGAAGCCAAACAAtg                                                                      >  2:1602537/1‑98 (MQ=255)
                           tACCACCCATGCTTTGAATCATCTGCAAATCAATAGCAAGCGTGGTCTGGTGTACACCGGTGATCACCAGTGGGGCGTACAGGAAGCCAAACAAtgctgc                                                                  <  1:1204713/100‑1 (MQ=255)
                           tACCACCCATGCTTTGAATCATCTGCAAATCAATAGCAAGCGTGGTCTGGTGTACACCGGTGATCACCAGTGGGGCGTACAGGAAGCCAAACAAtgctgc                                                                  >  2:2334736/1‑100 (MQ=255)
                            accaccCATGCTTTGAATCATCTGCAAATCAATAGCAAGCGTGGTCTGGTGTACACCGGTGATCACCAGTGGGGCGTACAGGAAGCCAAACAAtgctgc                                                                  >  2:1698063/1‑99 (MQ=255)
                            accaccCATGCTTTGAATCATCTGCAAATCAATAGCAAGCGTGGTCTGGTGTACACCGGTGATCACCAGTGGGGCGTACAGGAAGCCAAACAATGCTgcg                                                                 <  1:126128/100‑1 (MQ=255)
                             ccaccCATGCTTTGAATCATCTGCAAATCAATAGCAAGCGTGGTCTGGTGTACACCGGTGATCACCAGTGGGGCGTACAGGAAGCCAAACAATGCTgcgc                                                                >  1:3071427/1‑100 (MQ=255)
                              caccCATGCTTTGAATCATCTGCAAATCAATAGCAAGCGTGGTCTGGTGTACACCGGTGATCAACAGTGGGGCGTACAGGAAGCCAAACAATGCTgcg                                                                 >  2:3072362/1‑98 (MQ=255)
                               accCATGCTTTGAATCATCTGCAAATCAATAGCAAGCGTGGTCTGGTGTACACCGGTGATCACCAGTGGGGCGTACAGGAAGCCAAACAATGCTGCGCCa                                                              >  1:993241/1‑100 (MQ=255)
                                   aTGCTTTGAATCATCTGCAAATCAATAGCAAGCGTGGTCTGGTGTACACCGGTGATCACCAGTGGGGCGTACAGGAAGCCAAACAATGCTGCGCCAATCg                                                          <  1:1281051/100‑1 (MQ=255)
                                      cTTTGAATCATCTGCAAATCAATAGCAAGCGTGGTCTGGTGTACACCGGTGATCACCAGTGGGGCGTACAGGAAGCCAAACAATGCTGCGCCAATCGGAg                                                       <  1:1890889/100‑1 (MQ=255)
                                      cTTTGAATCATCTGCAAATCAATAGCAAGCGTGGTCTGGTGTACACCGGTGATCACCAGTGGGGCGTACAGGAAGCCAAACAATGCTGCGCCAATCGGAg                                                       <  1:336167/100‑1 (MQ=255)
                                       tttGAATCATCTGCAAATCAATAGCAAGTGTGGTCTGGTGTACACCGGTGATCACCAGTGGGGCGTACAGGAAGCCAAACAATGCTGCGCCAATCGGAg                                                       <  1:1652891/99‑1 (MQ=255)
                                       tttGAATCATCTGCAAATCAATAGCAAGCGTGGTCTGGTGTACACCGGTGATCACCAGTGGGGCGTACAGGAAGCCAAACAATGCTGCGCCAATCGGAGc                                                      <  2:924078/100‑1 (MQ=255)
                                           aaTCATCTGCAAATCAATAGCAAGCGTGGTCTGGTGTACACCGGTGATCACCAGTGGGGCGTACAGGAAGCCAAACAATGCTGCGCCAATCGGAGCAAAg                                                  <  1:2394749/100‑1 (MQ=255)
                                           aaTCATCTGCAAATCAATAGCAAGCGTGGTCTGGTGTACACCGGTGATCACCAGTGGGGCGTACAGGAAGCCAAACAATGCTGCGCCAATCGGAGCAAAg                                                  <  1:1516162/100‑1 (MQ=255)
                                           aaTCATCTGCAAATCAATAGCAAGCGTGGTCTGGTGTACACCGGTGATCACCAGTGGGGCGTACAGGAAGCCAAACAATGCTGCGCCAATCGGAGCAAAg                                                  <  1:1933021/100‑1 (MQ=255)
                                            atcatcTGCAAATCAATAGCAAGCGTGGTCTGGTGTACACCGGTGATCACCAGTGGGGCGTACAGGAAGCCAAACAATGCTGCGCCAATCGGAGCAAAGc                                                 <  2:2878449/100‑1 (MQ=255)
                                            atcatcTGCAAATCAATAGCAAGCGTGGTCTGGTGTACACCGGTGATCACCAGTGGGGCGTACAGGAAGCCAAACAATGCTGCGCCAATCGGAGCAAAGc                                                 >  1:1989451/1‑100 (MQ=255)
                                              catcTGCAAATCAATAGCAAGCGTGGTCTGGTGTACACCGGTGATCACCAGCGGGGCGTACAGGAAGCCAAACAATGCTGCGCCAATCGGAGCAAAg                                                  >  2:2174122/1‑97 (MQ=255)
                                                 cTGCAAATCAATAGCAAGCGTGGTCTGGTGTACACCGGTGATCACCAGTGGGGCGTACAGGAAGCCAAACAATGCTGCGCCAATCGGAGCAAAGCTGCCg                                            <  2:3439629/100‑1 (MQ=255)
                                                  tGCAAATCAATAGCAAGCGTGGTCTGGTGTACACCGGTGATCACCAGTGGGGCGTACAGGAAGCCAAACAATGCTGCGCCAATCGGAGCAAAGCTGCCgg                                           <  1:1039444/100‑1 (MQ=255)
                                                  tGCAAATCAATAGCAAGCGTGGTCTGGTGTACACCGGTGATCACCAGTGGGGCGTACAGGAAGCCAAACAATGCTGCGCCAATCGGAGCAAAGCTGCCgg                                           <  2:382692/100‑1 (MQ=255)
                                                  tGCAAATCAATAGCAAGCGTGGTCTGGTGTACACCGGTGATCACCAGTGGGGCGTACAGGAAGCCAAACAATGCTGCGCCAATCGGAGCAAAGCTGCCgg                                           <  1:3200662/100‑1 (MQ=255)
                                                  tGCAAATCAATAGCAAGCGTGGTCTGGTGTACACCGGTGATCACCAGTGGGGCGTACAGGAAGCCAAACAATGCTGCGCCAATCGGAGCAAAGCTGCCgg                                           >  2:2403504/1‑100 (MQ=255)
                                                  tGCAAATCAATAGCAAGCGTGGTCTGGTGTACACCGGTGATCACCAGTGGGGCGTACAGGAAGCCAAACAATGCTGCGCCAATCGGAGCAAAGCTGCCgg                                           >  2:1430930/1‑100 (MQ=255)
                                                  tGCAAATCAATAGCAAGCGTGGTCTGGTGTACACCGGTGATCACCAGTGGGGCGTACAGGAAGCCAAACAATGCTGCGCCAATCGGAGCAAAGCTGCCgg                                           >  1:2232549/1‑100 (MQ=255)
                                                    cAAATCAATAGCAAGCGTGGTCTGGTGTACACCGGTGATCACCAGTGGGGCGTACAGGAAGCCAAACAATGCTGCGCCAATCGGAGCAAAGCTGCCGGtc                                         >  1:1006094/1‑100 (MQ=255)
                                                     aaaTCAATAGCAAGCGTGGTCTGGTGTACACCGGTGATCACCAGTGGGGCGTACAGGAAGCCAAACAATGCTGCGCCAATCGGAGCAAAGCTGCCGGtca                                        <  1:2063/100‑1 (MQ=255)
                                                       aTCAATAGCAAGCGTGGTCTGGTGTACACCGGTGATCACCAGTGGGGCGTACAGGAAGCCAAACAATGCTGCGCCAATCGGAGCAAAGCTGCCGGtcatc                                      <  1:2108076/100‑1 (MQ=255)
                                                       aTCAATAGCAAGCGTGGTCTGGTGTACACCGGTGATCACCAGTGGGGCGTACAGGAAGCCAAACAATGCTGCGCCAATCGGAGCAAAGCTGCCGGtcatc                                      <  1:1742178/100‑1 (MQ=255)
                                                          aaTAGCAAGCGTGGTCTGGTGTACACCGGTGATCACCAGTGGGGCGTACAGGAAGCCAAACAATGCTGCGCCAATCGGAGCAAAGCTGCCGGTCATCAgg                                   >  2:1698302/1‑100 (MQ=255)
                                                           aTAGCAAGCGTGGTCTGGTGTACACCGGTGATCACCAGTGGGGCGTACAGGAAGCCAAACAATGCTGCGCCAATCGGAGCAAAGCTGCCGGTCATCAGGt                                  >  1:3224003/1‑100 (MQ=255)
                                                           aTAGCAAGCGTGGTCTGGTGTACACCGGTGATCACCAGTGGGGCGTACAGGAAGCCAAACAATGCTGCGCCAATCGGAGCAAAGCTGCCGGTCATCAGGt                                  >  1:389851/1‑100 (MQ=255)
                                                            tAGCAAGCGTGGTCTGGTGTACACCGGTGATCACCAGTGGGGCGTACAGGAAGCCAAAAAAAGCTGCGCCAATCGGAGCAAAGCTGCCAGtcagtcggtg                                 >  1:3426290/1‑93 (MQ=255)
                                                              gCAAGCGTGGTCTGGTGTACACCGGTGATCACCAGTGGGGCGTACAGGAAGCCAAACAATGCTGCGCCAATCGGAGCAAAGCTGCCGGTCATCAgatcgg                               >  1:138716/1‑95 (MQ=255)
                                                               cAAGCGTGGTCTGGTGTACACCGGTGATCACCAGTGGGGCGTACAGGAAGCCAAACAATGCTGCGCCAATCGGAGCAAAGCTGCCGGTCATCAGGTGACg                              <  1:2018253/100‑1 (MQ=255)
                                                                aaGCGTGGTCTGGTGTACACCGGTGATCACCAGTGGGGCGTACAGGAAGCCAAACAATGCTGCGCCAATCGGAGCAAAGCTGCCGGTCATCAGGTGACGt                             >  1:934399/1‑100 (MQ=255)
                                                                 aGCGTGGTCTGGTGTACACCGGTGATCACCAGTGGGGCGTACAGGAAGCCAAACAATGCTGCGCCAATCGGAGCAAAGCTGCCGGTCATCAGGTGACGTa                            <  2:160034/100‑1 (MQ=255)
                                                                  gCGTGGTCGGGGGTACACCGGTGATCACCAGTGGGGCGTACAGGAAGCCAAACAATGCTGCGCCAATCGGAGCAAAGATGCCGGTCATCAGGTGAc                               <  2:3178557/96‑1 (MQ=255)
                                                                   cGTGGTCTGGTGTACACCGGTGATCACCAGTGGGGCGTACAGGAAGCCAAACAATGCTGCGCCAATCGGAGCAAAGCTGCCGGTCATCAGGTGACGTAcc                          <  1:1808759/100‑1 (MQ=255)
                                                                      ggTCTGGTGTACACCGGTGATCACCAGTGGGGCGTACAGGAAGCCAAACAATGCTGCGCCAATCGGAGCAAAGCTGCCGGTCATCAGGTGACGTACCGCa                       >  1:2887841/1‑100 (MQ=255)
                                                                       gTCTGGTGTACACCGGTGATCACCAGTGGGGCGTACAGGAAGCCAAACAATGCTGCGCCAATCGGAGCAAAGCTGCCGGTCATCAGGTGACGTACCGCaa                      <  2:1970511/100‑1 (MQ=255)
                                                                         cTGGTGTACACCGGTGATCACCAGTGGGGCGTACAGGAAGCCAAACAATGCTGCGCCAATCGGAGCAAAGCTGCCGGTCATCAGGTGACGTACCGCAAAg                    <  1:253691/100‑1 (MQ=255)
                                                                         cTGGTGTACACCGGTGATCACCAGTGGGGCGTACAGGAAGCCAAACAATGCTGCGCCAATCGGAGCAAAGCTGCCGGTCATCAGGTGACGTACCGCAAAg                    >  1:507014/1‑100 (MQ=255)
                                                                          tGGTGTACACCGGTGATCACCAGTGGGGCGTACAGGAAGCCAAACAATGCTGCGCCAATCGGAGCAAAGCTGCCGGTCATCAGGTGACGTACCGCAAAgg                   >  2:2116009/1‑100 (MQ=255)
                                                                           ggTGTACACCGGTGAGCACCAGTGGGGCGTACAGGAAGCCAAACAATGCTGCGCCAATCGGAGCAAAGCTGCCGGTCATCAGGTGACGTACCGCAAAGGc                  >  2:3042863/1‑100 (MQ=255)
                                                                            gtgtACACCGGTGATCACCAGTGGGGCGTACAGGAAGCCAAACAATGCTGCGCCAATCGGAGCAAAGCTGCCGGTCATCAGGTGACGTACCGCAAAGGCa                 >  1:1441258/1‑100 (MQ=255)
                                                                            gtgtACACCGGTGATCACCAGTGGGGCGTACAGGAAGCCAAACAATGCTGCGCCAATCGGAGCAAAGCTGCCGGTCATCAGGTGACGTACCGCAAAGGCa                 >  1:622816/1‑100 (MQ=255)
                                                                             tgtACACCGGTGATCACCAGTGGGGCGTACAGGAAGCCAAACAATGCTGCGCCAATCGGAACAAAGCTGCCGGTCATCAGGTGACGTACCGCAAAGGCaa                >  1:2545158/1‑100 (MQ=255)
                                                                              ccccactggTGATCACCGGTGGGGCGTACAGGAAGCCAAACAATGCTGCGCCAATCGGAGCAAAGCTGCCGGTCATCAGGTGACGTACCGCAAAGGCAAc               >  1:3737193/8‑100 (MQ=255)
                                                                              ccccactggTGATCACCGGTGGGGCGTACAGGAAGCCAAACAATGCTGCGCCAATCGGAGCAAAGCTGCCGGTCATCAGGTGACGTACCGCAAAGGCAAc               >  2:907146/8‑100 (MQ=255)
                                                                              ccccacTGGTGATCACCGGTGGGGCGTACAGGAAGCCAAACAATGCTGCGCCAATCGGAGCAAATCTGCCGGTCATCAGGTGACGTACCGCAAAGGCaa                >  1:2079032/4‑99 (MQ=255)
                                                                                ccacTGGTGATCACCGGTGGGGCGTACAGGAAGCCAAACAATGCTGCGCCAATCGGAGCAAAGCTGCCGGTCATCAGGTGACGTACCGCAAAGGCAACGc             >  1:1598418/2‑100 (MQ=255)
                                                                                acacCGGTGATCACCAGTGGGGCGTACAGGAAGCCAAACAATGCTGCGCCAATCGGAGCAAAGCTGCCGGTCATCAGGTGACGTACCGCAAAGGCAACGc             >  1:3333548/1‑100 (MQ=255)
                                                                                 cagcGGTGATCACGAGCGGGGCGTACAGGAAGGCAAGCAAAGCTGCGCCAATCGGAGCTAAGCCGCCGGCCACCAGGTGACGCACCGCAAAGGCaagg              >  2:3192500/4‑96 (MQ=255)
                                                                                 cacCGGTGATCACCAGTGGGGCGTACAGGAAGCCAAACAATGCTGCGCCAATCGGAGCAAAGCTGCCTGTCATCAGGTGACGTACCGCAAAGGCAACGcc            >  2:766570/1‑100 (MQ=255)
                                                                                 cacCGGTGATCACCAGTGGGGCGTACAGGAAGCCAAACAATGCTGCGCCAATCGGAGCAAAGCTGCCGGTCATCAGGTGACGTACCGCAAAGGCAACGcc            >  2:2472443/1‑100 (MQ=255)
                                                                                 cacCGGTGATCACCAGTGGGGCGTACAGGAAGCCAAACAATGCTGCGCCAATCGGAGCAAAGCTGCCGGTCATCAGGTGACGTACCGCAAAGGCAACGcc            >  2:1448289/1‑100 (MQ=255)
                                                                                 cacCGGTGATCACCAGTGGGGCGTACAGGAAGCCAAACAATGCTGCGCCAATCGGAGCAAAGCTGCCGGTCATCAGGTGACGTACCGCAAAGGCAACGcc            <  2:2856349/100‑1 (MQ=255)
                                                                                 cacCGGTGATCACCAGTGGGGCGTACAGGAAGCCAAACAATGCTGCGCCAATCGGAGCAAAGCTGCCGGTCATCAGGTGACGTACCGCAAAGGCAACGcc            >  1:372526/1‑100 (MQ=255)
                                                                                  acCGGTGATCACCAGTGGGGCGTACAGGAAGCCAAACAATGCTGCGCCAATCGGAGCAAAGCTGCCGGTCATCAGGTGACGTACCGCAAAGGCAACGCCa           >  2:1290150/1‑100 (MQ=255)
                                                                                  acCGGTGATCACCAGCGGGGCGTACAGGAAGCCAAACAATGCTGCGCCAATCGGAGCAAAGCTGCCGGTCATCAGGTGACGTACCGCAAAGGCAACGCCa           >  1:3013601/1‑100 (MQ=255)
                                                                                   ccGGTGATCACCAGTGGGGCGTACAGGAAGCCAAACAATGCTGCGCCAATCGGAGCAAAGCTGCCGGTCATCAGGTGACGTACCGCAGAGGCAACGCCAt          >  2:1751071/1‑100 (MQ=255)
                                                                                   ccGGTGATCACCAGTGGGGCGTACAGGAAGCCAAACAATGCTGCGCCAATCGGAGCAAAGCTGCCGGTCATCAGGTGACGTACCGCAAAGGCAACGCCAt          >  1:713016/1‑100 (MQ=255)
                                                                                    cGGTGATCACCAGTGGGGCGTACAGGAAGCCAAACAATGCTGCGCCAATCGGAGCAAAGCTGCCGGTCATCAGGTGACGTACCGCAAAGGCAACGCCATc         >  2:3572869/1‑100 (MQ=255)
                                                                                      gTGATCACCAGTGGGGCGTACAGGAAGCCAAACAATGCTGCGCCAATCGGAGCAAAGCTGCCGGTCATCAGGTGACGTACCGCAAAGGCAACGCCATCGc       >  1:3509517/1‑100 (MQ=255)
                                                                                      gTGATCACCAGTGGGGCGTACAGGAAGCCAAACAATGCTGCGCCAATCGGAGCAAAGCTGCCGGTCATCAGGTGACGTACCGCAAAGGCAACGCCATCGc       >  2:1978704/1‑100 (MQ=255)
                                                                                       ttaTCACCAGTGGGGCGTACAGGAAGCCAAACAATGCTGCGCCAATCGGAGCAAAGCTGCCGGTCATCAGGTGACGTACCGCAAAGGCAACGCCATCGcc      >  1:3543374/3‑100 (MQ=255)
                                                                                        gATCACCAGTGGGGCGTACAGGAAGCCAAACAATGCTGCGCCAATCGGAGCAAAGCTGCCGGTCATCAGGTGACGTACCGCAAAGGCAACGCCATCGCCa     >  1:266597/1‑100 (MQ=255)
                                                                                          tCACCAGTGGGGCGTACAGGAAGCCAAACAATGCTGCGCCAATCGGAGCAAAGCTGCCGGTCATCAGGTGACGTACCGCAAAGGCAACGCCATCGCCAAt   <  1:1573974/100‑1 (MQ=255)
                                                                                          tCACCAGTGGGGCGTACAGGAAGCCAAACAATGCTGCGCCAATCGGAGCAAAGCTGCCGGTCATCAGGTGACGTACCGCAAAGGCAACGCCATCGCCAAt   >  1:2420600/1‑100 (MQ=255)
                                                                                           cACCAGTGGGGCGTACAGGAAGCCAAACAATGCTGCGCCAATCGGAGCAAAGCTGCCGGTCATCAGGTGACGTACCGCAAAGGCAACGCCATCGCCAATc  >  1:3569785/1‑100 (MQ=255)
                                                                                           cACCAGTGGGGCGTACAGGAAGCCAAACAATGCTGCGCCAATCGGAGCAAAGCTGCCGGTCATCAGGTGACGTACCGCAAAGGCAACGCCATCGCCAATc  <  1:451391/100‑1 (MQ=255)
                                                                                           cACCAGTGGGGCGTACAGGAAGCCAAACAATGCTGCGCCAATCGGAGCAAAGCTGCCGGTCATCAGGTGACGTACCGCAAAGGCAACGCCATCGCCAATc  >  1:1338047/1‑100 (MQ=255)
                                                                                                 |                                                                                             
ACAGCGCAATCAGCGGCCACACTGGCGTACCACCCATGCTTTGAATCATCTGCAAATCAATAGCAAGCGTGGTCTGGTGTACACCGGTGATCACCAGCGGGGCGTACAGGAAGCCAAACAATGCTGCGCCAATCGGAGCAAAGCTGCCGGTCATCAGGTGACGTACCGCAAAGGCAACGCCATCGCCAATC  >  NC_000913/4465139‑4465329

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A