Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A3 F110 I0 R1 60 98.0 4713732 96.7% 4558178 99.9

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 4,465,236 C→T 50.4% P315P (CCG→CCA)  treB ← trehalose‑specific PTS enzyme: IIB and IIC component

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,465,2360CT50.4% ‑0.7 / 158.6 103P315P (CCG→CCA) treBtrehalose‑specific PTS enzyme: IIB and IIC component
Reads supporting (aligned to +/- strand):  ref base C (29/22);  new base T (23/29);  total (52/51)
Fisher's exact test for biased strand distribution p-value = 2.39e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 4.10e-01

Only 100 of 105 total aligned reads displayed.

CGACAGCGCAATCAGCGGCCACACTGGCGTACCACCCATGCTTTGAATCATCTGCAAATCAATAGCAAGCGTGGTCTGGTGTACACCGGTGATCACCAGCGGGGCGTACAGGAAGCCAAACAATGCTGCGCCAATCGGAGCAAAGCTGCCGGTCATCAGGTGACGTACCGCAAAGGCAACGCCATCGCCAATCAT  >  NC_000913/4465137‑4465331
                                                                                                   |                                                                                               
cGACAGCGCAATCAGCGGCCACACTGGCGTACCACCCATGCTTTGAATCATCTGCAAATCAATAGCAAGCGTGGTCTGGTGTACACCGGTGATCACCAGc                                                                                                 >  1:425580/1‑100 (MQ=255)
cGACAGCGCAATCAGCGGCCACACTGGCGTACCACCCATGCTTTGAATCATCTGCAAATCAATAGCAAGCGTGGTCTGGTGTACACCGGTGATCACCAGc                                                                                                 >  2:570458/1‑100 (MQ=255)
    aGCGCAATCAGCGGCCACACTGGCGTACCACCCATGCTTTGAATCATCTGCAAATCAATAGCAAGCGTGGTCTGGTGTACACCGGTGATCACCAGTgggg                                                                                             <  1:1349704/100‑1 (MQ=255)
     gcgcAATCAGCGGCCACACTGGCGTACCACCCATGCTTTGAATCATCTGCAAATCAATAGCAAGCGTGGTCTGGTGTACACCGGTGATCACCAGTGGGGc                                                                                            <  1:345578/100‑1 (MQ=255)
     gcgcAATCAGCGGCCACACTGGCGTACCACCCATGCTTTGAATCATCTGCAAATCAATAGCAAGCGTGGTCTGGTGTACACCGGTGATCACCAGCgggg                                                                                             >  2:1418016/1‑99 (MQ=255)
     gcgcAATCAGCGGCCACACTGGCGTACCACCCATGCTTTGAATCATCTGCAAATCAATAGCAAGCGTGGTCTGGTGTACACCGGTGATCACCAGCGGGGc                                                                                            >  2:78835/1‑100 (MQ=255)
         aaTCAGCGGCCACACTGGCGTACCACCCATGCTTTGAATCATCTGCAAATCAATAGCAAGCGTGGTCTGGTGTACACCGGTGATCACCAGTGGGGCGtgc                                                                                        <  1:1595385/100‑3 (MQ=255)
          aTCAGCGGCCACACTGGCGTACCACCCATGCTTTGAATCATCTGCAAATCAATAGCAAGCGTGGTCTGGTGTACACCGGTGATCACCAGTGGGGCGTACa                                                                                       >  1:1278191/1‑100 (MQ=255)
           tCAGCGGCCACACTGGCGTACCACCCATGCTTTGAATCATCTGCAAATCAATAGCAAGCGTGGTCTGGTGTACACCGGTGATCACCAGTGGGGCGTACAg                                                                                      <  1:35156/100‑1 (MQ=255)
           tCAGCGGCCACACTGGCGTACCACCCATGCTTTGAATCATCTGCAAATCAATAGCAAGCGTGGTCTGGTGTACACCGGTGATCACCAGTGGGGCGTACAg                                                                                      >  1:622726/1‑100 (MQ=255)
            cAGCGGCCACACTGGCGTACCACCCATGCTTTGAATCATCTGCAAATCAATAGCAAGCGTGGTCTGGTGTACACCGGTGATCACCAGTGGGGCGTACAgg                                                                                     <  2:1552285/100‑1 (MQ=255)
            cAGCGGCCACACTGGCGTACCACCCATGCTTTGAATCATCTGCAAATCAATAGCAAGCGTGGTCTGGTGTACACCGGTGATCACCAGTGGGGCGTACAgg                                                                                     <  2:1464637/100‑1 (MQ=255)
            cAGCGGCCACACTGGCGTACCACCCATGCTTTGAATCATCTGCAAATCAATAGCAAGCGTGGTCTGGTGTACACCGGTGATCACCAGCGGGGCGTACAgg                                                                                     >  2:1423309/1‑100 (MQ=255)
             aGCGGCCACACTGGCGTACCACCCATGCTTTGAATCATCTGCAAATCAATAGCAAGCGTGGTCTGGTGTACACCGGTGATCACCAGTGGGGCGTACAGGa                                                                                    >  2:239054/1‑100 (MQ=255)
             aGCGGCCACACTGGCGTACCACCCATGCTTTGAATCATCTGCAAATCAATAGCAAGCGTGGTCTGGTGTACACCGGTGATCACCAGCGGGGCGTACAGGa                                                                                    >  1:1407643/1‑100 (MQ=255)
                ggCCACACTGGCGTACCACCCATGCTTTGAATCATCTGCAAATCAATAGCAAGCGTGGTCTGGTGTACACCGGTGATCACCAGTGGGGCGTACAGGAAGc                                                                                 <  1:889202/100‑1 (MQ=255)
                ggCCACACTGGCGTACCACCCATGCTTTGAATCATCTGCAAATCAATAGCAAGCGTGGTCTGGTGTACACCGGTGATCACCAGCGGGGCGTACAGGAAGc                                                                                 <  1:168441/100‑1 (MQ=255)
                    acacTGGCGTACCACCCATGCTTTGAATCATCTGCAAATCAATAGCAAGCGTGGTCTGGTGTACACCGGTGATCACCAGCGGGGCGTACAGGAAGCCaaa                                                                             >  1:1754640/1‑100 (MQ=255)
                     cacTGGCGTACCACCCATGCTTTGAATCATCTGCAAATCAATAGCAAGCGTGGTCTGGTGTACACCGGTGATCACCAGTGGGGCGTACAGGAAGCCAAAc                                                                            <  1:1984457/100‑1 (MQ=255)
                     cacTGGCGTACCACCCATGCTTTGAATCATCTGCAAATCAATAGCAAGCGTGGTCTGGTGTACACCGGTGATCACCAGCGGGGCGTACAGGAAGCCAAAc                                                                            <  1:268440/100‑1 (MQ=255)
                     cacTGGCGTACCACCCATGCTTTGAATCATCTGCAAATCAATAGCAAGCGTGGTCTGGTGTACACCGGTGATCACCAGCGGGGCGTACAGGAAGCCAAAc                                                                            <  2:1726781/100‑1 (MQ=255)
                         ggCGTACCACCCATGCTTTGAATCATCTGCAAATCAATAGCAAGCGTGGTCTGGTGTACACCGGTGATCACCAGTGGGGCGTACAGGAAGCCAAACAAtg                                                                        >  2:1723389/1‑100 (MQ=255)
                           cGTACCACCCATGCTTTGAATCATCTGCAAATCAATAGCAAGCGTGGTCTGGTGTACACCGGTGATCACCAGCGGGGCGTACAGGAAGCCAAACAAtgct                                                                      >  2:759244/1‑100 (MQ=255)
                             tACCACCCATGCTTTGAATCATCTGCAAATCAATAGCAAGCGTGGTCTGGTGTACACCGGTGATCACCAGTGGGGCGTACAGGAAGCCAAACAAtgctgc                                                                    >  1:698691/1‑100 (MQ=255)
                             tACCACCCATGCTTTGAATCATCTGCAAATCAATAGCAAGCGTGGTCTGGTGTACACCGGTGATCACCAGTGGGGCGTACAGGAAGCCAAACAAtgctgc                                                                    <  2:1150953/100‑1 (MQ=255)
                             tACCACCCAGGCTTTGAATCATCTGCAAATCAATAGCAAGCGTGGTCTGGTGTACACCGGTGATCACCAGCGGGGCGTACAGGAAGCCAAACAAtgctgc                                                                    <  2:536832/100‑1 (MQ=255)
                              accaccCATGCTTTGAATCATCTGCAAATCAATAGCAAGCGTGGTCTGGTGTACACCGGTGATCACCAGCGGGGCGTACAGGAAGCCAAACAATGCTgcg                                                                   >  2:1195056/1‑100 (MQ=255)
                               ccaccCATGCTTTGAATCATCTGCAAATCAATAGCAAGCGTGGTCTGGTGTACACCGGTGATCACCAGTGGGGCGTACAGGAAGCCAAACAATGCTgcgc                                                                  <  1:1144577/100‑1 (MQ=255)
                               ccaccCATGCTTTGAATCATCTGCAAATCAATAGCAAGCGTGGTCTGGTGTACACCGGTGATCACCAGTGGGGCGTACAGGAAGCCAAACAATGCTgcgc                                                                  <  1:1148253/100‑1 (MQ=255)
                               ccaccCATGCTTTGAATCATCTGCAAATCAATAGCAAGCGTGGTCTGGTGTACACCGGTGATCACCAGCGGGGCGTACAGGAAGCCAAACAATGCTgcgc                                                                  <  1:862543/100‑1 (MQ=255)
                                caccCATGCTTTGAATCATCTGCAAATCAATAGCAAGCGTGGTCTGGTGTACACCGGTGATCACCAGCGGGGCGTACAGGAAGCCAAACAATGCTGCGcc                                                                 <  2:1603194/100‑1 (MQ=255)
                                caccCATGCTTTGAATCATCTGCAAATCAATAGCAAGCGTGGTCTGGTGTACACCGGTGATCACCAGCGGGGCGTACAGGAAGCCAAACAATGCTGCGcc                                                                 >  1:328106/1‑100 (MQ=255)
                                   ccATGCTTTGAATCATCTGCAAATCAATAGCAAGCGTGGTCTTGTGTACACCGGTGATCACCAGCGGGGCGTACAGGAAGCCAAACAATGCTGCGCCAAt                                                              >  2:44250/1‑100 (MQ=255)
                                     aTGCTTTGAATCATCTGCAAATCAATAGCAAGCGTGGTCTGGTGTACACCGGTGATCACCAGTGGGGCGTACAGGAAGCCAAACAATGCTGCGCCAATCg                                                            <  1:981280/100‑1 (MQ=255)
                                     aTGCTTTGAATCATCTGCAAATCAATAGCAAGCGTGGTCTGGTGTACACCGGTGATCACCAGCGGGGCGTACAGGAAGCCAAACAATGCTGCGCCAATCg                                                            >  2:25598/1‑100 (MQ=255)
                                      tGCTTTGAATCATCTGCAAATCAATAGCAAGCGTGGTCTGGTGTACACCGGTGATCACCAGTGGGGCGTACAGGAAGCCAAACAATGCTGCGCCAATCgg                                                           >  2:2197569/1‑100 (MQ=255)
                                       gCTTTGAATCATCTGCAAATCAATAGCAAGCGTGGTCTGGTGTACACCGGTGATCACCAGCGGGGCGTACAGGAAGCCAAACAATGCTGCGCCAATCGGa                                                          <  1:574337/100‑1 (MQ=255)
                                        cTTTGAATCATCTGCAAATCAATAGCAAGCGTGGTCTGGTGTACACCGGTGATCACCAGCGGGGCGTACAGGAAGCCAAACAATGCTGCGCCAATCGGAg                                                         >  1:2033980/1‑100 (MQ=255)
                                         tttGAATCATCTGCAAATCAATAGCAAGCGTGGTCTGGTGTACACCGGTGATCACCAGTGGGGCGTACAGGAAGCCAAACAATGCTGCGCCAATCGGAGc                                                        <  1:2186347/100‑1 (MQ=255)
                                          ttGAATCATCTGCAAATCAATAGCAAGCGTGGTCTGGTGTACACCGGTGATCACCAGCGGGGCGTACAGGAAGCCAAACAATGCTGCGCCAATCGGAGCa                                                       >  2:1741718/1‑100 (MQ=255)
                                              atcatcTGCAAATCAATAGCAAGCGTGGTCTGGTGTACACCGGTGATCACCAGCGGGGCGTACAGGAAGCCAAACAATGCTGCGCCAATCGGAGCAAAGc                                                   <  1:2101859/100‑1 (MQ=255)
                                                natcTGCAAATCAATAGCAAGCGTGGTCTGGTGTACACCGGTGATCACCAGCGGGGCGTACAGGAAGCCAAACAATGCTGCGCCAATCGGAGCAAAGCTg                                                 >  1:237861/2‑100 (MQ=255)
                                                catcTGCAAATCAATAGCAAGCGTGGTCTGGTGTACACCGGTGATCACCAGTGGGGCGTACAGGAAGCCAAACAATGCTGCGCCAATCGGAGCAAAGCTg                                                 <  2:1679109/100‑1 (MQ=255)
                                                   cTGCAAATCAATAGCAAGCGTGGTCTGGTGTACACCGGTGATCACCAGTGGGGCGTACAGGAAGCCAAACAATGCTGCGCCAATCGGAGCAAAGCTGCCg                                              >  1:2254624/1‑100 (MQ=255)
                                                   cTGCAAATCAATAGCAAGCGTGGTCTGGTGTACACCGGTGATCACCAGCGGGGCGTACAGGAAGCCAAACAATGCTGCGCCAATCGGAGCAAAGCTGCCg                                              >  2:1206021/1‑100 (MQ=255)
                                                   cTGCAAATCAATAGCAAGCGTGGTCTGGTGTACACCGGTGATCACCAGCGGGGCGTACAGGAAGCCAAACAATGCTGCGCCAATCGGAGCAAAGCTGCCg                                              >  2:1014484/1‑100 (MQ=255)
                                                   cTGCAAATCAATAGCAAGCGGGGTCTGGTGTACACCGGTGATCACCAGCGGGGCGTACAGGAAGCCAAACAATGCTGCGCCAATCGGAGCAAAGCTGCCg                                              >  2:1434515/1‑100 (MQ=255)
                                                    tGCAAATCAATAGCAAGCGTGGTCTGGTGTACACCGGTGATCACCAGCGGGGCGTACAGGAAGCCAAACAATGCTGCGCCAATCGGAGCAAAGCTGCCgg                                             >  2:678131/1‑100 (MQ=255)
                                                     gCAAATCAATAGCAAGCGTGGTCTGGTGTACACCGGTGATCACCAGTGGGGCGTACAGGAAGCCAAACAATGCTGCGCCAATCGGAGCAAAGCTGCCGGt                                            >  1:400731/1‑100 (MQ=255)
                                                       aaaTCAATAGCAAGCGTGGTCTGGTGTACACCGGTGATCACCAGCGGGGCGTACAGGAAGCCAAACAATGCTGCGCCAATCGGAGCAAAGCTGCCGGtca                                          >  1:2274068/1‑100 (MQ=255)
                                                        aaTCAATAGCAAGCGTGGTCTGGTGTACACCGGTGATCACCAGCGGGGCGTACAGGAAGCCAAACAATGCTGCGCCAATCGGAGCAAAGCTGCCGGtcat                                         >  2:761959/1‑100 (MQ=255)
                                                         aTCAATAGCAAGCGTGGTCTGGTGTACACCGGTGATCACCAGTGTGGCGGACAGGAAGCCAAACAATGCGGCGCCAATCGGAGCAAAGCTTCCGGtcatc                                        <  2:1055342/100‑1 (MQ=255)
                                                         aTCAATAGCAAGCGTGGTCTGGTGTACACCGGTGATCACCAGCGGGGCGTACAGGAAGCCAAACAATGCTGCGCCAATCGGAGCAAAGCTGCCGGtcatc                                        <  2:216139/100‑1 (MQ=255)
                                                          tCAATAGCAAGCGTGGTCTGGTGTACACCGGTGATCACCAGTGGGGCGTACAGGAAGCCAAACAATGCTGCGCCAATCGGAGCAAAGCTGCCGGtcatca                                       <  1:499759/100‑1 (MQ=255)
                                                          tCAATAGCAAGCGTGGTCTGGTGTACACCGGTGATCACCAGCGGGGCGTACAGGAAGCCAAACAATGCTGCGCCAATCGGAGCAAAGCTGCCGGtcatca                                       <  2:21322/100‑1 (MQ=255)
                                                           cAATAGCAAGCGTGGTCTGGTGTACACCGGTGATCACCAGTGGGGCGTACAGGAAGCCAAACCATGCTGCGCCAATCGGAGCACAGCTGCCGGtcatccg                                      >  2:1873050/1‑98 (MQ=255)
                                                              tAGCAAGCGTGGTCTGGTGTACACCGGTGATCACCAGTGGGGCGTACAGGAAGCCAAACAATGCTGCGCCAATCGGAGCAAAGCTGCCGGTCATCAGGTg                                   <  1:435589/100‑1 (MQ=255)
                                                              tAGCAAGCGTGGTCTGGTGTACACCGGTGATCACCAGTGGGGCGTACAGGAAGCCAAACAATGCTGCGCCAATCGGAGCAAAGCTGCCGGTCATCAGGTg                                   <  1:2014275/100‑1 (MQ=255)
                                                              tAGCAAGCGTGGTCTGGTGTACACCGGTGATCACCAGCGGGGCGTACAGGAAGCCAAACAATGCTGCGCCAATCGGAGCAAAGCTGCCGGTCATCAGGTg                                   >  1:697131/1‑100 (MQ=255)
                                                              tAGCAAGCGTGGTCTGGTGTACACCGGTGATCACCAGCGGGGCGTACAGGAAGCCAAACAATGCTGCGCCAATCGGAGCAAAGCTGCCGGTCATCAGGTg                                   <  2:1340636/100‑1 (MQ=255)
                                                                gCAAGCGTGGTCTGGTGTACACCGGTGATCACTAGTGGGGGGTAGAGGAAGGCAAACAATGCTGCGCTAATCGGAGCAAAGCTGCCGGTCATCAGGTg                                   >  2:1956821/1‑98 (MQ=255)
                                                                gCAAGCGTGGTCTGGTGTACACCGGTGATCACCAGTGGGGCGTACAGGAAGCCAAACAATGCTGCGCCAATCGGAGCAAAGCTGCCGGTCATCAGGTGAc                                 >  2:1853096/1‑100 (MQ=255)
                                                                gCAAGCGTGGTCTGGTGTACACCGGTGATCACCAGTGGGGCGTACAGGAAGCCAAACAATGCTGCGCCAATCGGAGCAAAGCTGCCGGTCATCAGGTGAc                                 <  1:1047897/100‑1 (MQ=255)
                                                                 cAAGCGTGGTCTGGTGTACACCGGTGATCACCAGTGGGGCGTACAGGAAGCCAAACAATGCTGCGCCAATCGGAGCAAAGCTGCCGGTCATCAGGTGACg                                >  1:1039363/1‑100 (MQ=255)
                                                                 cAAGCGTGGTCTGGTGTACACCGGTGATCACCAGTGGGGCGTACAGGAAGCCAAACAATGCTGCGCCAATCGGAGCAAAGCTGCCGGTCATCAGGTGACg                                >  2:1621021/1‑100 (MQ=255)
                                                                   aGCGTGGTCTGGTGTACACCGGTGATCACCAGTGGGGCGTACAGGAAGCCAAACAATGCTGCGCCAATCGGAGCAAAGCTGCCGGTCATCAGGTGACGt                               >  1:1663458/1‑99 (MQ=255)
                                                                    gCGTGGTCTGGTGTACACCGGTGATCACCAGCGGGGCGTACAGGAAGCCAAACAATGCTGCGCCAATCGGAGCAAAGCTGCCGGTCATCAGGTGACGTAc                             <  2:1316334/100‑1 (MQ=255)
                                                                    gCGTGGTCTGGTGTACACCGGTGATCACCAGCGGGGCGTACAGGAAGCCAAACAATGCTGCGCCAATCGGAGCAAAGCTGCCGGTCATCAGGTGACGTAc                             <  1:78835/100‑1 (MQ=255)
                                                                    gCGTGGTCTGGTGTACACCGGTGATCACCAGCGGGGCGTACAGGAAGCCAAACAATGCTGCGCCAATCGGAGCAAAGCTGCCGGTCATCAGGTGACGTAc                             >  2:1705660/1‑100 (MQ=255)
                                                                       tGGTCTGGTGTACACCGGTGATCACCAGCGGGGCGTACAGGAAGCCAAACAATGCTGCGCCAATCGGAGCAAATCTGCCGGTCATCAGGTGACGTACCGc                          <  1:1432160/100‑1 (MQ=255)
                                                                        ggTCTGGTGTACACCGGTGATCACCAGTGGGGCGTACAGGAAGCCAAACAATGCTGCGCCAATCGGAGCAAAGCTGCCGGTCATCAGGTGACGTACCGCa                         <  1:1394512/100‑1 (MQ=255)
                                                                         gTCTGGTGTACACCGGTGATCACCAGTGGGGCGTACAGGAAGCCAAACAATGCTGCGCCAATCGGAGCAAAGCTGCCGGTCATCAGGTGACGTACCGCaa                        >  1:1411780/1‑100 (MQ=255)
                                                                           cTGGTGTACACCGGTGATCACCAGTGGGGCGTACAGGAAGCCAAACAATGCTGCGCCAATCGGAGCAAAGCTGCCGGTCATCAGGTGACGTACCGCAAAg                      <  2:2324285/100‑1 (MQ=255)
                                                                           cTGGTGTACACCGGTGATCACCAGTGGGGCGTACAGGAAGCCAAACAATGCTGCGCCAATCGGAGCAAAGCTGCCGGTCATCAGGTGACGTACCGCAAAg                      <  2:400728/100‑1 (MQ=255)
                                                                            tGGTGTACACCGGTGATCACCAGCGGGGCGTACAGGAAGCCAAACAATGCTGCGCCAATCGGAGCAAAGCTGCCGGTCATCAGGTGACGTACCGCAAAgg                     <  1:415871/100‑1 (MQ=255)
                                                                             ggTGTACACCGGTGATCACCAGTGGGGCGTACAGGAAGCCAAACAATGCTGCGCCAATCGGAGCAAAGCTGCCGGTCATCAGGTGACGTACCGCAAAGGc                    >  2:775725/1‑100 (MQ=255)
                                                                             ggTGTACACCGGTGATCACCAGCGGGGCGTACAGGAAGCCAAACAATGCTGCGCCAATCGGAGCAAAGCTGCCGGTCATCAGGTGACGTACCGCAAAGGc                    >  2:1422576/1‑100 (MQ=255)
                                                                              gtgtACACCGGTGATCACCAGTGGGGCGTACAGGAAGCCAAACAATGCTGCGCCAATCGGAGCAAAGCTGCCGGTCATCAGGTGACGTACCGCAAAGGCa                   <  1:1908924/100‑1 (MQ=255)
                                                                                gtACACCGGTGATCACCAGTGGGGCGTACAGGAAGCCAAACAATGCTGCGCCAATCGGAGCAAAGCTGCCGGTCATCAGGTGACGTACCGCAAAGGCAAc                 <  1:2139009/100‑1 (MQ=255)
                                                                                ccccactggTGATCACCGGTGGGGCGTACAGGAAGCCAAACAATGCTGCGCCAATCGGAGCAAAGCTGCCGGTCATCAGGTGACGTACCGCAAAGGCAAc                 >  1:766439/8‑100 (MQ=255)
                                                                                 tACACCGGTGATCACCAGCGGGGCGTACAGGAAGCCAAACAATGCTGCGCCAATCGGAGCAAAGCTGCCGGTCATCAGGTGACGTACCGCAAAGGCAACg                <  1:1423317/100‑1 (MQ=255)
                                                                                  acacCGGTGATCACCAGTGGGGCGTACAGGAAGCCAAACAATGCTGCGCCAATCGGAGCAAAGCTGCCGGTCATCAGGTGACGTACCGCAAAGGCAACGc               <  1:1396326/100‑1 (MQ=255)
                                                                                  acacCGGTGATCACCAGCGGGGCGTACAGGAAGCCAAACAATGCTGCGCCAATCGGAGCAAAGCTGCCGGTCATCAGGTGACGTACCGCAAAGGCAACGc               <  2:1754627/100‑1 (MQ=255)
                                                                                   cacCGGTGATCACCAGTGGGGCGTACAGGAAGCCAAACAATGCTGCGCCAATCGGAGCAAAGCTGCCGGTCATCAGGTGACGTACCGCAAAGGCAACGcc              >  1:516395/1‑100 (MQ=255)
                                                                                   cacCGGTGATCACCAGCGGGGCGTACAGGAAGCCAAACAATGCTGCGCCAATCGGAGCAAAGCTGCCGGTCATCAGGTGACGTACCGCAAAGGCAACGcc              <  2:1407635/100‑1 (MQ=255)
                                                                                   cacCGGTGATCACCAGCGGGGCGTACAGGAAGCCAAACAATGCTGCGCCAATCGGAGCAAAGCTGCCGGTCATCAGGTGACGTACCGCAAAGGCAACGcc              <  1:2025592/100‑1 (MQ=255)
                                                                                       ggTGATCACCAGTGGGGCGTACAGGAAGCCAAACAATGCTGCGCCAATCGGAGCAAAGCTGCCGGTCATCAGGTGACGTACCGCAAAGGCAACGCCATCg          >  1:348091/1‑100 (MQ=255)
                                                                                        gTGATCACCAGCGGGGCGTACAGGAAGCCAAACAATGCTGCGCCAATCGGAGCAAAGCTGCCGGTCATCAGGTGACGTACCGCAAAGGCAACGCCATCGc         >  1:1348591/1‑100 (MQ=255)
                                                                                         tGATCACCAGTGGGGCGTACAGGAAGCCAAACAATGCTGCGCCAATCGGAGCAAAGCTGCCGGTCATCAGGTGACGTACCGCAAAGGCAACGCCATCGcc        >  2:1914283/1‑100 (MQ=255)
                                                                                         tGATCACCAGCGGGGCGTACAGGAAGCCAAACAATGCTGCGCCAATCGGAGCAAAGCTGCCGGTCATCAGGTGACGTACCGCAAAGGCAACGCCATCGcn        <  2:237860/100‑2 (MQ=255)
                                                                                         tGATCACCAGCGGGGCGTACAGGAAGCCAAACAATGCTGCGCCAATCGGAGCAAAGCTGCCGGTCATCAGGTGACGTACCGCAAAGGCAACGCCATCGcc        >  2:1799876/1‑100 (MQ=255)
                                                                                            tCACCAGTGGGGCGTACAGGAAGCCAAACAATGCTGCGCCAATCGGAGCAAAGCTGCCGGTCATCAGGTGACGTACCGCAAAGGCAACGCCATCGCCAAt     >  1:318004/1‑100 (MQ=255)
                                                                                            tCACCAGTGGGGCGTACAGGAAGCCAAACAATGCTGCGCCAATCGGAGCAAAGCTGCCGGTCATCAGGTGACGTACCGCAAAGGCAACGCCATCGCCAAt     <  2:851364/100‑1 (MQ=255)
                                                                                            tCACCAGCGGGGCGTACAGGAAGCCAAACAATGCTGCGCCAATCGGAGCAAAGCTGCCGGTCATCAGGTGACGTACCGCAAAGGCAACGCCCTCGCCAAt     >  1:1477354/1‑100 (MQ=255)
                                                                                            tCACCAGCGGGGCGTACAGGAAGCCAAACAATGCTGCGCCAATCGGAGCAAAGCTGCCGGTCATCAGGTGACGTACCGCAAAGGCAACGCCATCGCCaa      >  1:728474/1‑99 (MQ=255)
                                                                                            tCACCAGCGGGGCGTACAGGAAGCCAAACAATGCTGCGCCAATCGGAGCAAAGCTGCCGGTCATCAGGTGACGTACCGCAAAGGCAACGCCATCGCCAAt     >  1:1474725/1‑100 (MQ=255)
                                                                                            tCACCAGCGGGGCGTACAGGAAGCCAAACAATGCTGCGCCAATCGGAGCAAAGCTGCCGGTCATCAGGTGACGTACCGCAAAGGCAACGCCATCGCCAAt     >  1:1302832/1‑100 (MQ=255)
                                                                                              aCCAGTGGGGCGTACAGGAAGCCAAACAATGCTGCGCCAATCGGAGCAAAGCTGCCGGTCATCAGGTGACGTACCGCAAAGGCAACGCCATCGCCAATCa   <  1:900388/100‑1 (MQ=255)
                                                                                               ccAGTGGGGCTTACAGGAAGCCAAACAATGCTGCGCCAATCGGAGCAAAGCTGCCGGTCATCAGGTGACGTACCGCGAAGGCAACGCCATCGCCAATCa   <  2:64818/99‑1 (MQ=255)
                                                                                               ccAGCGGGGCGTACAGGAAGCCAAACAATGCTGCGCCAATCGGAGCAAAGCTGCCGGTCATCAGGTGACGTACCGCAAAGGCAACGCCATCGCCAATCAt  <  2:425577/100‑1 (MQ=255)
                                                                                                   |                                                                                               
CGACAGCGCAATCAGCGGCCACACTGGCGTACCACCCATGCTTTGAATCATCTGCAAATCAATAGCAAGCGTGGTCTGGTGTACACCGGTGATCACCAGCGGGGCGTACAGGAAGCCAAACAATGCTGCGCCAATCGGAGCAAAGCTGCCGGTCATCAGGTGACGTACCGCAAAGGCAACGCCATCGCCAATCAT  >  NC_000913/4465137‑4465331

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A