Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A8 F232 I1 R1 72 114.8 5816246 94.5% 5496352 99.9

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 4,465,236 C→T P315P (CCG→CCA)  treB ← trehalose‑specific PTS enzyme: IIB and IIC component

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,465,2360CT99.1% 383.2 / ‑4.7 114P315P (CCG→CCA) treBtrehalose‑specific PTS enzyme: IIB and IIC component
Reads supporting (aligned to +/- strand):  ref base C (1/0);  new base T (65/48);  total (66/48)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.44e-01

Only 100 of 114 total aligned reads displayed.

ACAGCGCAATCAGCGGCCACACTGGCGTACCACCCATGCTTTGAATCATCTGCAAATCAATAGCAAGCGTGGTCTGGTGTACACCGGTGATCACCAGCGGGGCGTACAGGAAGCCAAACAATGCTGCGCCAATCGGAGCAAAGCTGCCGGTCATCAGGTGACGTACCGCAAAGGCAACGCCATCGCCAATCAT  >  NC_000913/4465139‑4465331
                                                                                                 |                                                                                               
aCAGCGCAATCAGCGGCCACACTGGCGTACCACCCATGCTTTGAATCATCTGCAAATCAATAGCAAGCGTGGTCTGGTGTACACCGGTGATCACCAGTgg                                                                                               <  1:2179648/100‑1 (MQ=255)
aCAGCGCAATCAGCGGCCACACTGGCGTACCACCCATGCTTTGAATCATCTGCAAATCAATAGCAAGCGTGGTCTGGTGTACACCGGTGATCACCAGTgg                                                                                               <  1:2113055/100‑1 (MQ=255)
 cAGCGCAATCAGCGGCCACACTGGCGTACCACCCATGCTTTGAATCATCTGCAAATCAATAGCAAGCGTGGTCTGGTGTACACCGGTGATCACCAGTggg                                                                                              >  2:1991787/1‑100 (MQ=255)
 cAGCGCAATCAGCGGCCACACTGGCGTACCACCCATGCTTTGAATCATCTGCAAATCAATAGCAAGCGTGGTCTGGTGTACACCGGTGATCACCAGTggg                                                                                              >  2:2609222/1‑100 (MQ=255)
 cAGCGCAATCAGCGGCCACACTGGCGTACCACCCATGCTTTGAATCATCTGCAAATCAATAGCAAGCGTGGTCTGGTGTACACCGGTGATCACCAGTggg                                                                                              >  1:2225431/1‑100 (MQ=255)
 cAGCGCAATCAGCGGCCACACTGGCGTACCACCCATGCTTTGAATCATCTGCAAATCAATAGCAAGCGTGGTCTGGTGTACACCGGTGATCACCAGTggg                                                                                              >  1:2092893/1‑100 (MQ=255)
 cAGCGCAATCAGCGGCCACACTGGCGTACCACCCATGCTTTGAATCATCTGCAAATCAATAGCAAGCGCGGTCTGGTGTACACCGGTGATCACCAGTggg                                                                                              <  2:1951632/100‑1 (MQ=255)
   gcgcAATCAGCGGCCACACTGGCGTACCACCCATGCTTTGAATCATCTGCAAATCAATAGCAAGCGTGGTCTGGTGTACACCGGTGATCACCAGTGGGGc                                                                                            >  2:2722442/1‑100 (MQ=255)
    cgcAATCAGCGGCCACACTGGCGTACCACCCATGCTTTGAATCATCTGCAAATCAATAGCAAGCGTGGTCTGGTGTACACCGGTGATCACCAGTGGGGCg                                                                                           <  1:2571764/100‑1 (MQ=255)
      cAATCAGCGGCCACACTGGCGTACCACCCATGCTTTGAATCATCTGCAAATCAATAGCAAGCGTGGTCTGGTGTACACCGGTGATCACCAGTGGGGCGTa                                                                                         >  1:2372558/1‑100 (MQ=255)
      cAATCAGCGGCCACACTGGCGTACCACCCATGCTTTGAATCATCTGCAAATCAATAGCAAGCGTGGTCTGGTGTACACCGGTGATCACCAGTGGGGCGTa                                                                                         >  2:2824400/1‑100 (MQ=255)
       aaTCAGCGGCCACACTGGCGTACCACCCACGCTTTGAATCATCTGCAAATCAATAGCAAGCGTGGTCTGGTGTACACCGGTGATCACCAGTGGGGCGTAc                                                                                        <  2:926715/100‑1 (MQ=255)
        aTCAGCGGCCACACTGGCGTACCACCCATGCTTTGAATCATCTGCAAATCAATAGCAAGCGTGGTCTGGTGTACACCGGTGATCACCAGTGGGGCGTAc                                                                                        <  2:927180/99‑1 (MQ=255)
        aTCAGCGGCCACACTGGCGTACCACCCATGCTTTGAATCATCTGCAAATCAATAGCAAGCGTGGTCTGGTGTACACCGGTGATCACCAGTGGGGCGTACa                                                                                       <  2:115439/100‑1 (MQ=255)
        aTCAGCGGCCACACTGGCGTACCACCCATGCTTTGAATCATCTGCAAATCAATAGCAAGCGTGGTCTGGTGTACACCGGTGATCACCAGTGGGGCGTACa                                                                                       >  1:2228527/1‑100 (MQ=255)
         tCAGCGGCCACACTGGCGTACCACCCATGCTTTGAATCATCTGCAAATCAATAGCAAGCGTGGTCTGGTGTACACCGGTGATCACCAGTGGGGCGTACAg                                                                                      <  2:1173705/100‑1 (MQ=255)
           aGCGGCCACACTGGCGTACCACCCATGCTTTGAATCATCTGCAAATCAATAGCAAGCGTGGTCTGGTGTACACCGGTGATCACCAGTGGGGCGTACAGGa                                                                                    >  2:137766/1‑100 (MQ=255)
           aGCGGCCACACTGGCGTACCACCCATGCTTTGAATCATCTGCAAATCAATAGCAAGCGTGGTCTGGTGTACACCGGTGATCACCAGTGGGGCGTACAGGa                                                                                    <  1:1479894/100‑1 (MQ=255)
           aGCGGCCACACTGGCGTACCACCCATGCTTTGAATCATCTGCAAATCAATAGCAAGCGTGGTCTGGTGTACACCGGTGATCACCAGTGGGGCGTACAGGa                                                                                    >  1:418555/1‑100 (MQ=255)
            gCGGCCACACTGGCGTACCACCCATGCTTTGAATCATCTGCAAATCAATAGCAAGCGTGGTCTGGTGTACACCGGAGATCACCAGTGGGGCGTACAGGaa                                                                                   <  1:2609189/100‑1 (MQ=255)
              ggCCACACTGGCGTACCACCCATGCTTTGAATCATCTGCAAATCAATAGCAAGCGTGGTCTGGTGTACACCGGTGATCACCAGTGGGGCGTACAGGAAGc                                                                                 >  2:1842603/1‑100 (MQ=255)
              ggCCACACTGGCGTACCACCCATGCTTTGAATCATCTGCAAATCAATAGCAAGCGTGGTCTGGTGTACACCGGTGATCACCAGTCGGGCGTACAGGAAGc                                                                                 >  2:2855966/1‑100 (MQ=255)
               nccACACTGGCGTACCACCCATGCTTTGAATCATCTGCAAATCAATAGCAAGCNTGGTCTGGTGTACACCGGTGATCACCANTGGGGCGTACAGNAAGcc                                                                                >  1:1029930/2‑100 (MQ=255)
               gCCACACTGGCGTACCACCCATGCTTTGAATCATCTGCAAATCAATAGCAAGCGTGGTCTGGTGTACACCGGTGATCACCAGTGGGGCGTACAGGAAGcc                                                                                >  1:2411139/1‑100 (MQ=255)
                 cacaCTGGCGTACCACCCATGCTTTGAATCATCTGCAAATCAATAGCAAGCGTGGTCTGGTGTACACCGGTGATCACCAGTGGGGCGTACAGGAAGCCaa                                                                              >  1:736147/1‑100 (MQ=255)
                   cacTGGCGTACCACCCATGCTTTGAATCATCTGCAAATCAATAGCAAGCGTGGTCTGGTGTACACCGGTGATCACCAGTGGGGCGTACAGGAAGCCAAAc                                                                            <  2:1757354/100‑1 (MQ=255)
                   cacTGGCGTACCACCCATGCTTTGAATCATCTGCAAATCAATAGCAAGCGTGGTCTGGTGTACACCGGTGATCACCAGTGGGGCGTACAGGAAGCCAAAc                                                                            <  2:295936/100‑1 (MQ=255)
                   cacTGGCGTACCACCCATGCTTTGAATCATCTGCAAATCAATAGCAAGCGTGGTCTGGTGTACACCGGTGATCACCAGTGGGGCGTACAGGAAGCCAAAc                                                                            >  1:2581995/1‑100 (MQ=255)
                     cTGGCGTACCACCCATGCTTTGAATCATCTGCAAATCAATAGCAAGCGTGGTCTGGTGTACACCGGTGATCACCAGTGGGGCGTACAGGAAGCCAAACaa                                                                          >  2:1013163/1‑100 (MQ=255)
                      tGGCGTACCACCCATGCTTTGAATCATCTGCAAATCAATAGCAAGCGTGGTCTGGTGTACACCGGTGATCACCAGTGGGGCGTACAGGAAGCCAAACAAt                                                                         <  2:2852536/100‑1 (MQ=255)
                        gCGTACCACCCATGCTTTGAATCATCTGCAAATCAATAGCAAGCGTGGTCTGGTGTACACCGGTGATCACCAGTGGGGCGTACAGGAAGCCAAACAAtgc                                                                       <  1:1319536/100‑1 (MQ=255)
                        gCGTACCACCCATGCTTTGAATCATCTGCAAATCAATAGCAAGCGTGGTCTGGTGTACACCGGTGATCACCAGTGGGGCGTACAGGAAGCCAAACAAtgc                                                                       <  1:1319537/100‑1 (MQ=255)
                          gTACCACCCATGCTTTGAATCATCTGCAAATCAATAGCAAGCGTGGTCTGGTGTACACCGGTGATCACCAGTGGGGCGTACAGGAAGCCAAACAAtgctg                                                                     >  1:25930/1‑100 (MQ=255)
                            accaccCATGCTTTGAATCATCTGCAAATCAATAGCAAGCGTGGTCTGGTGTACACCGGTGATCACCAGTGGGGCGTACAGGAAGCCAAACAATGCTgcg                                                                   <  2:813576/100‑1 (MQ=255)
                              caccCATGCTTTGAATCATCTGCAAATCAATAGCAAGCGTGGTCTGGTGTACACCGGTGATCACCAGTGGGGCGTACAGGAAGCCAAACAATGCTGCGcc                                                                 >  1:1510518/1‑100 (MQ=255)
                              caccCATGCTTTGAATCATCTGCAAATCAATAGCAAGCGTGGTCTGGTGTACACCGGTGATCACCAGTGGGGCGTACAGGAAGCCAAACAATGCTGCGcc                                                                 <  2:1727535/100‑1 (MQ=255)
                              caccCATGCTTTGAATCATCTGCAAATCAATAGCAAGCGTGGTCTGGTGTACACCGGTGATCACCAGTGGGGCGTACAGGAAGCCAAACAATGCTGCGcc                                                                 >  1:547786/1‑100 (MQ=255)
                              caccCATGCTTTGAATCATCTGCAAATCAATAGCAAGCGTGGTCTGGTGTACACCGGTGATCACCAGTCGGGCGTACAGGAAGCCAAACAATGCTGCGcc                                                                 >  2:182003/1‑100 (MQ=255)
                                cccATGCTTTGAATCATCTGCAAATCAATAGCAAGCGTGGTCTGGTGTACACCGGTGATCACCAGTGGGGCGTACAGGAAGCCAAACAATGCTGCGCCaa                                                               >  2:2734542/1‑100 (MQ=255)
                                  cATGCTTTGAATCATCTGCAAATCAATAGCAAGCGTGGTCTGGTGTACACCGGTGATCACCAGTGGGGCGTACAGGAAGCCAAACAATGCTGCGCCAATc                                                             >  2:1792542/1‑100 (MQ=255)
                                  cATGCTTTGAATCATCTGCAAATCAAAAGCAAGCGTGGTCTGGTGTACACCGGTGATCACCAGTGGGGCGTACAGGAAGCCAAGCATTGCTGCGCCAAt                                                              <  2:1907160/99‑1 (MQ=255)
                                      cTTTGAATCATCTGCAAATCAATAGCAAGCGTGGTCTGGTGTACACCGGTGATCACCAGTGGGGCGTACAGGAAGCCAAACAATGCTGCGCCAATCGGAg                                                         >  1:652946/1‑100 (MQ=255)
                                      cTTTGAATCATCTGCAAATCAATAGCAAGCGTGGTCTGGTGTACACCGGTGATCACCAGTGGGGCGTACAGGAAGCCAAACAATGCTGCGCCAATCGGAg                                                         <  2:1137231/100‑1 (MQ=255)
                                       tttGAATCATCTGCAAATCAATAGCAAGCGTGGTCTGGTGTACACCGGTGATCACCAGTGGGGCGTACAGGAAGCCAAACAATGCTGCGCCAATCGGAGc                                                        <  2:2795084/100‑1 (MQ=255)
                                        ttGAATCATCTGCAAATCAATAGCAAGCGTGGTCTGGTGTACACCGGTGATCACCAGTGGGGCGTACAGGAAGCCAAACAATGCTGCGCCAATCGGAGCa                                                       >  2:1597751/1‑100 (MQ=255)
                                         tGAATCATCTGCAAATCAATAGCAAGCGTGGTCTGGTGTACACCGGTGATCACCAGTGGGGCGTACAGGAAGCCAAACAATGCTGCGCCAATCGGAGCaa                                                      >  2:387018/1‑100 (MQ=255)
                                         tGAATCATCTGCAAATCAATAGCAAGCGTGGTCTGGTGTACACCGGTGATCACCAGTGGGGCGTACAGGAAGCCAAACAATGCTGCGCCAATCGGAGCaa                                                      >  2:550334/1‑100 (MQ=255)
                                           aaTCATCTGCAAATCAATAGCAAGCGTGGTCTGGTGTACACCGGTGATCACCAGTGGGGCGTACAGGAAGCCAAACAATGCTGCGCCAATCGGAGCAAAg                                                    <  1:1423860/100‑1 (MQ=255)
                                           aaTCATCTGCAAATCAATAGCAAGCGTGGTCTGGTGTACACCGGTGATCACCAGTGGGGCGTACAGGAAGCCAAACAATGCTGCGCCAATCGGAGCAAAg                                                    <  2:2216859/100‑1 (MQ=255)
                                            atcatcTGCAAATCAATAGCAAGCGTGGTCTGGTGTACACCGGTGATCACCAGTGGGGCGTACAGGAAGCCAAACAATGCTGCGCCAATCGGAGCAAAGc                                                   >  1:815136/1‑100 (MQ=255)
                                            atcatcTGCAAATCAATAGCAAGCGTGGTCTGGTGTACACCGGTGATCACCAGTGGGGCGTACAGGAAGCCAAACAATGCTGCGCCAATCGGAGCAAAGc                                                   >  1:2413184/1‑100 (MQ=255)
                                             tcatcTGCAAATCAATAGGAAGCGTGGGCTGGTGTACACCGGTGATCACCAGTGGGGCGTACAGGAAGCCAAACAATGCTGCGCCAATCGGAGCAAAg                                                    <  1:2282863/98‑1 (MQ=255)
                                             tcatcTGCAAATCAATAGCAAGCGTGGTCTGGTGTACACCGGTGATCACCAGTGGGGCGTACAGGAAGCCAAACAATGCTGCGCCAATCGGAGCAAAGCt                                                  >  2:2583373/1‑100 (MQ=255)
                                              catcTGCAAATCAATAGCAAGCGTGGTCTGGTGTACACCGGTGATCACCAGTGGGGCGTACAGGAAGCCAAACAATGCTGCGCCAATCGGAGCAAAGCt                                                  <  1:960141/99‑1 (MQ=255)
                                                tcTGCAAATCAATAGCAAGCGTGGTCTGGTGTACACCGGTGATCACCAGTGGGGCGTACAGGAAGCCAAACAATGCTGCGCCAATCGGAGCAAAGCTGcc                                               <  1:2574518/100‑1 (MQ=255)
                                                 cTGCAAATCAATAGCAAGCGTGGTCTGGTGTACACCGGTGATCACCAGTGGGGCGTACAGGAAGCCAAACAATGCTGCGCCAATCGGAGCAAAGCTGCCg                                              <  1:1079502/100‑1 (MQ=255)
                                                  tGCAAATCAATAGCAAGCGTGGTCTGGTGTACACCGGTGATCACCAGTGGGGCGTACAGGAAGCCAAACAATGCTGCGCCAATCGGAGCAAAGCTGCCgg                                             >  2:2214403/1‑100 (MQ=255)
                                                  tGCAAATCAATAGCAAGCGTGGTCTGGTGTACACCGGTGATCACCAGTGGGGCGTACAGGAAGCCAAACAATGCTGCGCCAATCGGAGCAAAGCTGCCgg                                             >  1:296778/1‑100 (MQ=255)
                                                   gCAAATCAATAGCAAGCGTGGTCTGGTGTACACCGGTGATCACCAGTGGGGCGTACAGGAAGCCAAACAATGCTGCGCCAATCGGAGCAAAGCTGCCGGt                                            <  1:1013152/100‑1 (MQ=255)
                                                    cAAATCAATAGCAAGCGTGGTCTGGTGTACACCGGTGATCACCAGTGGGGCGTACAGGAAGCCAAACAATGCTGCGCCAACCGGAGCAAAGCTGCCGGtc                                           >  1:2480230/1‑100 (MQ=255)
                                                     aaaTCAATAGCAAGCGTGGTCTGGTGTACACCGGTGATCACCAGTGGGGCGTACAGGAAGCCAAACAATGCTGCGCCAATCGGAGCAAAGCTGCCGGtca                                          <  2:1510542/100‑1 (MQ=255)
                                                      aaTCAATAGCAAGCGTGGTCTGGTGTACACCGGTGATCACCAGTGGGGCGTACAGGAAGCCAAACAATGCTGCGCCAATCGGAGCAAAGCTGCCGGtcat                                         >  1:1007198/1‑100 (MQ=255)
                                                       aTCAATAGCAAGCGTGGTCTGGTGTACACCGGTGATCACCAGTGGGGCGTACAGGAAGCCAAACAATGCTGCGCCAATCGGAGCAAAGCTGCCGGtcatc                                        >  2:2407523/1‑100 (MQ=255)
                                                        tCAATAGCAAGCGTGGTCTGGTGTACACCGGTGATCACCAGCGGGGCGTACAGGAAGCCAAACAATGCTGCGCCAATCGGAGCAAAGCTGCCGGtcatca                                       >  1:1843525/1‑100 (MQ=255)
                                                          aaTAGCAAGCGTGGTCTGGTGTACACCGGTGATCACCAGTGGGGCGTACAGGAAGCCAAACAATGCTGCGCCAATCGGAGCAAAGCTGCCGGTCATCAgg                                     <  1:1101369/100‑1 (MQ=255)
                                                           aTAGCAAGCGTGGTCTGGTGTACACCGGTGATCACCAGTGGGGCGTACAGGAAGCCAAACAATGCTGCGCCAATCGGAGCAAAGCTGCCGGTCATCAGGt                                    >  2:259460/1‑100 (MQ=255)
                                                            tAGCAAGCGTGGTCTGGTGTACACCGGTGATCACCAGTGGGGCGTACAGGAAGCCAAACAATGCTGCGCCAATCGGAGCAAAGCTGCCGGTCATCAGGTg                                   >  1:1045263/1‑100 (MQ=255)
                                                             aGCAAGCGTGGTCTGGTGTACACCGGTGATCACCAGTGGGGCGTACAGGAAGCCAAACAATGCTGCGCCAATCGGAGCAAAGCTGCCGGTCATCAGGTGa                                  <  2:652952/100‑1 (MQ=255)
                                                              gCAAGCGTGGTCTGGTGTACACCGGTGATCACCAGTGGGGCGTACAGGAAGCCAAACAATGCTGCGCCAATCGGAGCAAAGCTGCCGGTCATCAGGTGAc                                 <  1:2722406/100‑1 (MQ=255)
                                                              gCAAGCGTGGTCTGGTGTACACCGGTGATCACCAGTGGGGCGTACAGGAAGCCAAACAATGCTGCGCCAATCGGAGCAAAGCTGCCGGTCATCAGGTGAc                                 >  1:1061843/1‑100 (MQ=255)
                                                              gCAAGCGTGGTCTGGTGTACACCGGTGATCACCAGTGGGGCGTACAGGAAGCCAAACAATGCTGCGCCAATCGGAGCAAAGCTGCCGGTCATCAGGTGAc                                 >  2:2891294/1‑100 (MQ=255)
                                                                 aGCGTGGTCTGGTGTACACCGGTGATCACCAGTGGGGCGTACAGGAAGCCAAACAATGCTGCGCCAATCGGAGCAAAGCTGCCGGTCATCAGGTGACGTa                              >  1:438686/1‑100 (MQ=255)
                                                                  gCGTGGTCTGGTGTACACCGGTGATCACCAGTGGGGCGTACAGGAAGCCAAACAATGCTGCGCCAATCGGAGCAAAGCTGCCGGTCATCAGGTGACGTAc                             <  1:2855928/100‑1 (MQ=255)
                                                                   cGTGGTCTGGTGTACACCGGTGATCACCAGTGGGGCGTACAGGAAGCCAAACAATGCTGCGCCAATCGGAGCAAAGCTGCCGGTCATCAGGTGACGTAcc                            <  1:36219/100‑1 (MQ=255)
                                                                   cGTGGTCTGGTGTACACCGGTGATCACCAGTGGGGCGTACAGGAAGCCAAACAATGCTGCGCCAATCGGAGCAAAGCTGCCGGTCATCAGGTGACGTAcc                            >  2:1312716/1‑100 (MQ=255)
                                                                    gTGGTCTGGTGTACACCGGTGATCACCAGTGGGGCGTACAGGAAGCCAAACAATGCTGCGCCAATCGGAGCAAAGCTGCCGGTCATCAGGTGACGTACCg                           >  1:2872216/1‑100 (MQ=255)
                                                                    gTGGTCTGGTGTACACCGGTGATCACCAGTGGGGCGTACAGGAAGCCAAACAATGCTGCGCCAATCGGAGCAAAGCTGCCGGTCATCAGGTGACGTACCg                           <  2:1128585/100‑1 (MQ=255)
                                                                    gTGGTCTGGTGTACACCGGTGATCACCAGTGGGGCGTACAGGAAGCCAAACAATGCTGCGCCAATCGGAGCAAAGCTGCCGGTCATCAGGTGACGTACCg                           <  1:2540788/100‑1 (MQ=255)
                                                                     tGGTCTGGTGTACACCGGTGATCACCAGTGGGGCGTACAGGAAGCCAAACAATGCTGCGCCAATCGGAGCAAAGCTGCCGGTCATCAGGTGACGCACCGc                          <  2:2870969/100‑1 (MQ=255)
                                                                       gTCTGGTGTACACCGGTGATCACCAGTGGGGCGTACAGGAAGCCAAACAATGCTGCGCCAATCGGAGCAAAGCTGCCGGTCATCAGGTGACGTACCGCaa                        >  1:1139528/1‑100 (MQ=255)
                                                                         cTGGTGTACACCGGTGATCACCAGTGGGGCGTACAGGAAGCCAAACAATGCTGCGCCAATCGGAGCAAAGCTGCCGGTCATCAGGTGACGTACCGCAAAg                      >  2:1315252/1‑100 (MQ=255)
                                                                         cTGGTGTACACCGGTGATCACCAGTGGGGCGTACAGGAAGCCAAACAATGCTGCGCCAATCGGAGCAAAGCTGCCGGTCATCAGGTGACGTACCGCAAAg                      <  1:1597726/100‑1 (MQ=255)
                                                                           ggTGTACACCGGTGATCACCAGTGGGGCGTACAGGAAGCCAAACAATGCTGCGCCAATCGGAGCAAAGCTGCCGGTCATCAGGTGACGTACCGCAAAGGc                    >  2:2904243/1‑100 (MQ=255)
                                                                           ggTGTACACCGGTGATCACCAGTGGGGCGTACAGGAAGCCAAACAATGCTGCGCCAATCGGAGCAAAGATGCCGGTCATCAGGTGACGTACCGCAAAGGc                    >  1:2481256/1‑100 (MQ=255)
                                                                            gtgtACACCGGAGATCACCAGTGGGGCGTACAGGAAGCCAAACAATGCTGCGCCAATCGGAGCAAAGCTGCCGGTCATCAGGTGACATACCGCAAAGACa                   >  1:699021/1‑100 (MQ=255)
                                                                               tACACCGGTGATCACCAGTGGGGCGTACAGGAAGCCAAACAATGCTGCGCCAATCGGAGCAAAGCTGCCGGTCATCAGGTGACGTACCGCAAAGGCAACg                >  2:1073601/1‑100 (MQ=255)
                                                                                 cacCGGTGATCACCAGTGGGGCGTACAGGAAGCCAAACAATGCTGCGCCAATCGGAGCAAAGCTGCCGGTCATCAGGTGACGTACCGCAAAGGCAACGcc              <  2:2139997/100‑1 (MQ=255)
                                                                                 cacCGGTGATCACCAGTGGGGCGTACAGGAAGCCAAACAATGCTGCGCCAATCGGAGCAAAGCTGCCGGTCATCAGGTGACGTACCGCAAAGGCAACGcc              >  1:1362620/1‑100 (MQ=255)
                                                                                   ccGGTGATCACCAGTGGGGCGTACAGGAAGCCAAACAATGCTGCGCCAATCGGAGCAAAGCTGCCGGTCATCAGGTGACGTACCGCAAAGGCAACGCCAt            >  1:856688/1‑100 (MQ=255)
                                                                                   ccGGTGATCACCAGTGGGGCGTACAGGAAGCCAAACAATGCTGCGCCAATCGGAGCAAAGCTGCCGGTCATCAGGTGACGTACCGCAAAGGCAACGCCAt            >  1:2322289/1‑100 (MQ=255)
                                                                                     ggTGATCACCAGTGGGGCGTACAGGAAGCCAAACAATGCTGCGCCAATCGGAGCAAAGCTGCCGGTCATCAGGTGACGTACCGCAAAGGCAACGCCATCg          <  2:1052207/100‑1 (MQ=255)
                                                                                      gTGATCACCAGTGGGGCGTACAGGAAGCCAAACAATGCTGCGCCAATCGGAGCAAAGCTGCCGGTCATCAGGTGACGTACCGCAAAGGCAACGCCATCGc         >  2:511813/1‑100 (MQ=255)
                                                                                      gTGATCACCAGTGGGGCGTACAGGAAGCCAAACAATGCTGCGCCAATCGGAGCAAAGCTGCCGGTCATCAGGTGACGTACCGCAAAGGCAACGCCATCGc         >  2:290143/1‑100 (MQ=255)
                                                                                       tGATCACCAGTGGGGCGTACAGGAAGCCAAACAATGCTGCGCCAATCGGAGCAAAGCTGCCGGTCATCAGGTGACGTACCGCAAAGGCAACGCCATCGcc        >  2:2062570/1‑100 (MQ=255)
                                                                                          tCACCAGTGGGGCGTACAGGAAGCCAAACAATGCTGCGCCAATCGGAGCAAAGCTGCCGGTCATCAGGTGACGTACCGCAAAGGCAACGCCATCGCCAAt     >  2:1222028/1‑100 (MQ=255)
                                                                                           cACCAGTGGGGCGTACAGGAAGCCAAACAATGCTGCGCCAATCGGAGCAAAGCTGCCGGTCATCAGGTGACGTACCGCAAAGGCAACGCCATCGCCAATc    <  2:1045276/100‑1 (MQ=255)
                                                                                            aCCAGTGGGGCGTACAGGAAGCCAAACAATGCTGCGCCAATCGGAGCAAAGCTGCCGGTCATCAGGTGACGTACCGCAAAGGCAACGCCATCGCCAATCa   >  2:1378997/1‑100 (MQ=255)
                                                                                            aCCAGTGGGGCGTACAGGAAGCCAAACAATGCTGCGCCAATCGGAGCAAAGCTGCCGGTCATCAGGTGACGTACCGCAAAGGCAACGCCATCGCCAATCa   <  2:241616/100‑1 (MQ=255)
                                                                                             ccAGTGGGGCGTACAGGAAGCCAAACAATGCTGCGCCAATCGGAGCAAAGCTGCCGGTCATCAGGTGACGTACCGCAAAGGCAACGCCATCGCCAATCAt  >  1:2207966/1‑100 (MQ=255)
                                                                                             ccAGTGGGGCGTACAGGAAGCCAAACAATGCTGCGCCAATCGGAGCAAAGCTGCCGGTCATCAGGTGACGTACCGCAAAGGCAACGCCATCGCCAATCAt  >  2:2437053/1‑100 (MQ=255)
                                                                                                 |                                                                                               
ACAGCGCAATCAGCGGCCACACTGGCGTACCACCCATGCTTTGAATCATCTGCAAATCAATAGCAAGCGTGGTCTGGTGTACACCGGTGATCACCAGCGGGGCGTACAGGAAGCCAAACAATGCTGCGCCAATCGGAGCAAAGCTGCCGGTCATCAGGTGACGTACCGCAAAGGCAACGCCATCGCCAATCAT  >  NC_000913/4465139‑4465331

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A