Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A4 F53 I1 R1	64	112.2	5657101	94.0%	5317674	99.9

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,573,528	A→G	pseudogene (1115/1503 nt)	yjiT →	pseudogene

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,573,528	0	A	G	99.1%	403.0 / ‑4.4	111	pseudogene (1115/1503 nt)	yjiT	pseudogene
Reads supporting (aligned to +/- strand): ref base A (0/1); new base G (54/55); total (54/57)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.08e-01

Only 100 of 112 total aligned reads displayed.

AAAAGCGAAGTGAGATTTGGCAGGCAAAATCCATCGGCAGGTTTGTCGTTAGTGGCTCGTGCTGGAGGGATGATTGTTGGGAGCATTAAACTTGATGACAGTGAAATTGCCATTGGTGAGGTGCCGTTAACCTTCATCGTTGATGCAGATCAATGGCTGTTACAGGGACAGGCTTCTTGCAGTGTGCGAAGC  >  NC_000913/4573431‑4573622

    gCGAAGTGAGATTTGGCAGGCAAAATCCATCGGCAGGTTTGTCGTTAGTGGCTCGTGCTGGAGGGATGATTGTTGGGAGCATTAAACTTGATGGCAGTGa                                                                                          >  1:1505494/1‑100 (MQ=255)

        aGTGAGATTTGGCAGGCAAAATCCATCGGCAGGTTTGTCGTTAGTGGCTCGTGCTGGAGGGATGATTGTTGGGAGCATTAAACTTGATGGCAGTGAAAtt                                                                                      <  1:1145132/100‑1 (MQ=255)

         gTGAGATTTGGCAGGCAAAATCCATCGGCAGGTTTGTCGTTAGTGGCTCGTGCTGGAGGGATGATTGTTGGGAGCATTAAACTTGATGGCAGTGAAATTg                                                                                     >  1:1935323/1‑100 (MQ=255)

          tGAGATTTGGCAGGCAAAATCCATCGGCAGGTTTGTCGTTAGTGGCTCGTGCTGGAGGGATGATTGTTGGGAGCATTAAACTTGATGGCAGTGAAATTGc                                                                                    >  2:2174200/1‑100 (MQ=255)

                ttGGCAGGCAAAATCCATCGGCAGGTTTGTCGTTAGTGGCTCGTGCTGGAGGGATGATTGTTGGGAGCATTAAACTTGATGGCAGTGAAATTGCCATTgg                                                                              >  2:565169/1‑100 (MQ=255)

                  ggcaggcaAAATCCATCGGCAGGTTTGTCGTTAGTGGCTCGTGCTGGAGGGATGATTGTTGGGAGCATTAAACTTGATGTCAGTGAAATTGCCATTGGTg                                                                            <  1:1733597/100‑1 (MQ=255)

                  ggcaggcaAAATCCATCGGCAGGTTTGTCGTTAGTGGCTCGTGCTGGAGGGATGATTGTTGGGAGCATTAAACTTGATGGCAGTGAAATTGCCATTGGTg                                                                            <  1:1226537/100‑1 (MQ=255)

                   gcaggcaAAATCCATCGGCAGGTTTGTCGTTAGTGGCTCGTGCTGGAGGGATGATTGTTGGGAGCATTAAACTTGATGGCAGTGAAATTGCCATTGGTGa                                                                           <  2:1797226/100‑1 (MQ=255)

                   gcaggcaAAATCCATCGGCAGGTTTGTCGTTAGTGGCTCGTGCTGGAGGGATGATTGTTGGGAGCATTAAACTTGATGGCAGTGAAATTGCCATTGGTGa                                                                           >  2:1007213/1‑100 (MQ=255)

                    caggcaAAATCCATCGGCAGGTTTGTCGTTAGTGGCTCGTGCTGGAGGGATGATTGTTGGGAGCATTAAACTTGATGGCAGTGAAATTGCCATTGGTGAg                                                                          >  2:2467054/1‑100 (MQ=255)

                    caggcaAAATCCATCGGCAGGTTTGTCGTTAGTGGCTCGTGCTGGAGGGATGATTGTTGGGAGCATTAAACTTGATGGCAGTGAAATTGCCATTGGTGAg                                                                          >  1:1628598/1‑100 (MQ=255)

                     aggcaAAATCCATCTGCAGGTTTGTCGTTAGTGTCTCGTGCTGTAGGGATTATTGTTGGGATCATTAAACTTGATTGCATTGAAATGGCCATTTGTGatg                                                                         <  1:2694453/100‑3 (MQ=255)

                     aggcaAAATCCATCGGCAGGTTTGTCGTTAGTGGCTCGTGCTGGAGGGATGATTGTTGGGAGCATTAAACTTGATGGCAGTGAAATTGCCATTGGTGAgg                                                                         >  2:1304659/1‑100 (MQ=255)

                      ggcaAAATCCATCGGCAGGTTTGTCGTTAGTGGCTCGTGCTAGAGGGATGATTGTTGGGAGAATTAAACTTGATGGCAGTGAAATTGCCATTGGTGAgg                                                                         >  1:1546954/1‑99 (MQ=255)

                       ncaAAATCCATCGGCAGGTTTGTCGTTAGTGGCTCGTGCTGGAGGGATGATTGTTGGGAGCATTAAACTTGATGGCAGTGAAATTGCCATTGGTGAGGTg                                                                       >  1:2382583/2‑100 (MQ=255)

                       gcaAAATCCATCGGCAGGTTTGTCGTTAGTGGCTCGTGCTGGAGGGTTGATTGTCGGGAGCATTAAACTTGATGGCAGTGAAATTGCCATTGGTGAGGTg                                                                       >  1:14536/1‑100 (MQ=255)

                       gcaAAATCCATCGGCAGGTTTGTCGTTAGTGGCTCGTGCTGGAGGGATGATTGTTGGGAGCATTAAACTTGATGGCAGTGAAATTGCCATTGGTGAGGTg                                                                       >  2:2151581/1‑100 (MQ=255)

                        caAAATCCATCGGCAGGTTTGTCGTTAGTGGCTCGTGCTGGAGGGATGATTGTTGGGAGCATTAAACTTGATGGCAGTGAAATTGCCATTGGTGAGGTGc                                                                      >  2:2463894/1‑100 (MQ=255)

                        caAAATCCATCGGCAGGTTTGTCGTTAGTGGCTCGTGCTGGAGGGATGATTGTTGGGAGCATTAAACTTGATGGCAGTGAAATTGCCATTGGTGAGGTGc                                                                      <  1:2463874/100‑1 (MQ=255)

                         aaaaTCCATCGGCAGGTTTGTCGTTAGTGGCTCGTGCTGGAGGGATGATTGTTGGGAGCATTAAACTTGATGGCAGTGAAATTGCCATTGGTGAGGTGcc                                                                     <  1:1462764/100‑1 (MQ=255)

                          aaaTCCATCGGCAGGTTTGTCGTTAGTGGCTCGTGCTGGAGGGATGATTGTTGGGAGCATTAAACTTGATGGCAGTGAAATTGCCATTGGTGAGGTGCCg                                                                    <  1:172932/100‑1 (MQ=255)

                           aaTCCATCGGCAGGTTTGTCGTTAGTGGCTCGTGCTGGAGGGATGATTGTTGGGAGCATTAAACTTGATGGCAGTGAAATTGCCATTGGTGAGGTGCCGt                                                                   <  1:113903/100‑1 (MQ=255)

                            aTCCATCGGCAGGTTTGTCGTTAGTGGCTCGTGCTGGAGGGATGATTGTTGGGAGCATTAAACTTGATGGCAGTGAAATTGCCATTGGTGAGGTGCCGtt                                                                  <  1:926520/100‑1 (MQ=255)

                            aTCCATCGGCAGGTTTGTCGTTAGTGGCTCGTGCTGGAGGGATGATTGTTGGGAGCATTAAACTTGATGGCAGTGAAATTGCCATTGGTGAGGTGCCGtt                                                                  <  1:2125234/100‑1 (MQ=255)

                             tCCATCGGCAGGTTTGTCGTTAGTGGCTCGTGCTGGAGGGATGATTGTTGGGAGCATTAAACTTGATGGCAGTGAAATTGCCATTGGTGAGGTGCCGTTa                                                                 <  1:752730/100‑1 (MQ=255)

                              ccATCGGCAGGTGTGTCGTTAGTGGCTCGGGCTGGAGGGATGGTTGTTGGGAGCATTAAACTTGATGGCAGGGAAATTGCGATTGGGGAGGTGCCGTTaa                                                                >  1:2358753/1‑100 (MQ=255)

                               cATCGGCAGGTTTGTCGTTAGTGGCTCGTGCTGGAGGGATGATTGTTGGGAGCATTAAACTTGATGGCAGTGAAATTGCCATTGGTGAGGTGCCGTTAAc                                                               >  2:203579/1‑100 (MQ=255)

                                aTCGGCAGGTTTGTCGTTAGTGGCTCGTGCTGGAGGGATGATTGTTGGGAGCATTAAACTTGATGGCAGTGAAATTGCCATTGGTGAGGTGCCGTTAAcc                                                              <  2:1964866/100‑1 (MQ=255)

                                aTCGGCAGGTTTGTCGTTAGTGGCTCGTGCTGGAGGGATGATTGTAGGGAGCATTAAACTTGATGGCGGTGAAATTGCCATTGGGGAGGTGCCGTTAAcc                                                              >  2:2446813/1‑100 (MQ=255)

                                 tCGGCAGGTTTGTCGTTAGTGGCTCGTGCTGGAGGGATGATTGTTGGGAGCATTAAACTTGATGGCAGTGAAATTGCCATTGGTGAGGTGCCGTTAACCt                                                             <  2:1884235/100‑1 (MQ=255)

                                   ggCAGGTTTGTCGTTAGTGGCTCGTGCTGGAGGGATGATTGTTGGGAGCATTAAACTTGATGGCAGTGAAATTGACATTGGTGAGGTGCCGTTAACCTTc                                                           >  1:2434791/1‑100 (MQ=255)

                                    gCAGGTTTGTCGTTAGTGGCTCGTGCTGGAGGGATGATTGTTGGGAGCATTAAACTTGATGGCAGTGAAATTGCCATTGGTGAGGTGCCGTTAACCTTCa                                                          <  1:2066190/100‑1 (MQ=255)

                                     cAGGTTTGTCGTTAGTGGCTCGTGCTGGAGGGATGATTGTTGGGAGCATTAAACTTGATGGCAGTGAAATTGCCATTGGTGAGGTGCCGTTAACCTTCAt                                                         <  1:2225214/100‑1 (MQ=255)

                                     cAGGTTTGTCGTTAGTGGCTCGTGCTGGAGGGATGATTGTTGGGAGCATTAAACTTGATGGCAGTGAAATTGCCATTGGTGAGGTGCCGTTAACCTTCAt                                                         <  2:1252116/100‑1 (MQ=255)

                                     cAGGTTTGTCGTTAGTGGCTCGTGCTGGAGGGATGATTGTTGGGAGCATTAAACTTGATGGCAGTGAAATTGCCATTGGTGAGGTGCCGTTAACCTTCAt                                                         >  1:100904/1‑100 (MQ=255)

                                      aGGTTTGTCGTTAGTGGCTCGTGCTGGAGGGATGATTGTTGGGAGCATTAAACTTGATGGCAGTGAAATTGCCATTGGTGAGGTGCCGTTAACCTTCATc                                                        <  1:573870/100‑1 (MQ=255)

                                       ggTTTGTCGTTAGTGGCTCGTGCTGGAGGGATGATTGTTGGGAGCATTAAACTTGATGGCAGTGAAATTGCCATTGGTGAGGTGCCGTTAACCTTCATCg                                                       <  1:783723/100‑1 (MQ=255)

                                         tttGTCGTTAGTGGCTCGTGCTGGAGGGATGATTGTTGGGAGCATTAAACTTGATGGCAGTGAAATTGCCATTGGTGAGGTGCCGTTAACCTTCATCGtt                                                     <  2:1048482/100‑1 (MQ=255)

                                         tttGTCGTTAGTGGCTCGTGCTGGAGGGATGATTGTTGGGAGCATTAAACTTGATGGCAGTGAAATTGCCATTGGTGAGGTGCCGTTAACCTTCATCGtt                                                     <  1:1017809/100‑1 (MQ=255)

                                         tttGTCGTTAGTGGCTCGTGCTGGAGGGATGATTGTTGGGAGCATTAAACTTGATGGCAGTGAAATTGCCATTGGTGAGGTGCCGTTAACCTTCATCGtt                                                     <  1:2077180/100‑1 (MQ=255)

                                          ttGTCGTTAGTGGCTCGTGCTGGAGGGATGATTGTTGGGAGCATTAAACTTGATGGCAGTGAAATTGCCATTGGTGAGGTGCCGTTAACCTTCATCGtt                                                     <  1:2761473/99‑1 (MQ=255)

                                          ttGTCGTTAGTGGCTCGTGCTGGAGGGATGATTGTTGGGAGCATTAAACTTGATGGCAGTGAAATTGCCATTGGTGAGGTGCCGTTAACCTTCATCGTTg                                                    >  2:2239771/1‑100 (MQ=255)

                                          ttGTCGTTAGTGGCTCGTGCTGGAGGGATGATTGTTGGGAGCATTAAACTTGATGGCAGTGAAATTGCCATTGGTGAGGTGCCGTTAACCTTCATCGTTg                                                    <  2:128310/100‑1 (MQ=255)

                                           tGTCGTTAGTGGCTCGTGCTGGAGGGATGATTGTTGGGAGCATTAAACTTGATGGCAGTGAAATTGCCATTGGTGAGGTGCCGTTAACCTTCATCGTTGa                                                   >  2:2017769/1‑100 (MQ=255)

                                           tGTCGTTAGTGGCTCGTGCTGGAGGGATGATTGTTGGGAGCATTAAACTTGATGGCAGTGAAATTGCCATTGGTGAGGTGCCGTTAACCTTCATCGTTGa                                                   >  2:701661/1‑100 (MQ=255)

                                           tGTCGTTAGTGGCTCGTGCTGGAGGGATGATTGTTGGGAGCATTAAACTTGATGGCAGTGAAATTGCCATTGGTGAGGTGCCGTTAACCTTCATCGTTGa                                                   >  2:705770/1‑100 (MQ=255)

                                            gTCGTTAGTGGCTCGTGCTGGAGGGATGATTGTTGGGAGCATTAAACTTGATGGCAGTGAAATTGCCATTGGTGAGGTGCCGTTAACCTTCATCGTTGAt                                                  >  1:2098425/1‑100 (MQ=255)

                                             tCGTTAGTGGCTCGTGCTGGAGGGATGATTGTTGGGAGCATTAAACTTGATGGCAGTGAAATTGCCATTGGTGAGGTGCCGTTAACCTTCATCGTTGATg                                                 <  1:2017754/100‑1 (MQ=255)

                                             tCGTTAGTGGCTCGTGCTGGAGGGATGATTGTTGGGAGCATTAAACTTGATGGCAGTGAAATTGCCATTGGTGAGGTGCCGTTAACCTTCATCGTTGATg                                                 <  1:298357/100‑1 (MQ=255)

                                               gTTAGTGGCTCGTGCTGGAGGGATGATTGTTGGGAGCATTAAACTTGATGGCAGTGAAATTGCCATTGGTGAGGTGCCGTTAACCTTCATCGTTGATGCa                                               <  1:1304651/100‑1 (MQ=255)

                                               gTTAGTGGCTCGTGCTGGAGGGATGATTGTTGGGAGCATTAAACTTGATGGCAGTGAAATTGCCATTGGTGAGGTGCCGTTAACCTTCATCGTTGATGCa                                               <  2:2634420/100‑1 (MQ=255)

                                                 tAGTGGCTCGTGCTGGAGGGATGATTGTTGGGAGCATTAAACTTGATGGCAGTGAAATTGCCATTGGTGAGGTGCCGTTAACCTTCATCGTTGATGCAGa                                             >  2:1483444/1‑100 (MQ=255)

                                                  aGTGGCTCGTGCTGGAGGGATGATTGTTGGGAGCATTAAACTTGATGGCAGTGAAATTGCCATTGGTGAGGTGCCGTTAACCTTCATCGTTGATGCAGAt                                            <  1:2467034/100‑1 (MQ=255)

                                                  aGTGGCTCGTGCTGGAGGGATGATTGTTGGGAGCATTAAACTTGATGGCAGTGAAATTGCCATTGGTGAGGTGCCGTTAACCTTCATCGTTGATGCAGAt                                            <  2:1124389/100‑1 (MQ=255)

                                                    tGGCTCGTGCTGGAGGGATGATTGTTGGGAGCATTAAACTTGATGGCAGTGAAATTGCCATTGGTGAGGTGCCGTTAACCTTCATCGTTGATGCAGATCa                                          >  1:225381/1‑100 (MQ=255)

                                                       cTCGTGCTGGAGGGATGATTGTTGGGAGCATTAAACTTGATGGCAGTGAAATTGCCATTGGTGAGGTGCCGTTAACCTTCATCGTTGATGCAGATCAATg                                       >  2:87475/1‑100 (MQ=255)

                                                       cTCGTGCTGGAGGGATGATTGTTGGGAGCATTAAACTTGATGGCAGTGAAATTGCCATTGGTGAGGTGCCGTTAACCTTCATCGTTGATGCAGATCAATg                                       <  1:2151566/100‑1 (MQ=255)

                                                         cGTGCTGGAGGGATGATTGTTGGGAGCATTAAACTTGATGGCAGTGAAATTGCCATTGGTGAGGTGCCGTTAACCTTCATCGTTGATGCAGATCAATGGc                                     <  1:497549/100‑1 (MQ=255)

                                                         cGTGCTGGAGGGATGATTGTTGGGAGCATTAAACTTGATGGCAGTGAAATTGCCAGTGGTGAGGTGCCGTTAACCTTCATCGTTGATGCAGATCAATGGc                                     >  2:1687551/1‑100 (MQ=255)

                                                          gTGCTGGAGGGATGATTGTTGGGAGCATTAAACTTGATGGCAGTGAAATTGCCATTGGTGAGGTGCCGTTAACCTTCATCGTTGATGCAGATCAATGGCt                                    <  2:1024687/100‑1 (MQ=255)

                                                           tGCTGGAGGGATGATTGTTGGGAGCATTAAACTTGATGGCAGTGAAATTGCCATTGGTGAGGTGCCGTTAACCTTCATCGTTGATGCAGATCAATGGCTg                                   <  1:1299168/100‑1 (MQ=255)

                                                            ncTGGAGGGATGATTGTTGGGAGCATTACACTTGATGGCAGTGAAATTGCCATTGGTGAGGAGCCGTTAACCTTCATCGTTGATGCAGATCAATGGCTGt                                  >  1:379601/2‑100 (MQ=255)

                                                            ncTGGAGGGATGATTGTTGGGAGCATTAAACTTGATGGCAGTGAAATTGCCATTGGTGAGGTGCCGTTAACCTTCATCGTTGATGCAGATCAATGGCTGt                                  >  2:1596925/2‑100 (MQ=255)

                                                            gCTGGAGGGATGATTGTTGGGAGCATTAAACTTGATGGCAGTGAAATTGCCATTGGTGAGGTGCCGTTAACCTTCATCGTTGATGCAGATCAATGGCTGt                                  >  2:751476/1‑100 (MQ=255)

                                                            gCTGGAGGGATGATTGTTGGGAGCATTAAACTTGATGGCAGTGAAATTGCCATTGGTGAGGTGCCGTTAACCTTCATCGTTGATGCAGATCAATGGCTGt                                  <  2:1628610/100‑1 (MQ=255)

                                                             cTGGAGGGATGATTGTTGGGAGCATTAAACTTGATGGCAGTGAAATTGCCATTGGTGAGGTGCCGTTAACCTTCATCGTTGATGCAGATCAATGGCTGtt                                 >  1:1885503/1‑100 (MQ=255)

                                                             cTGGAGGGATGATTGTTGGGAGCATTAAACTTGATGGCAGTGAAATTGCCATTGGTGAGGTGCCGTTAACCTTCATCGTTGACGCAGATCAATGGCTGtt                                 >  1:2484660/1‑100 (MQ=255)

                                                              tGGAGGGATGATTGTTGGGACCATTAAACTTGATGGCAGTGAAATTGCCATTGGTGAGGTGCCGTTAACCTTCATCGTTGATGCAGATCAATGGCTGTTa                                >  1:1724948/1‑100 (MQ=255)

                                                               ggAGGGATGATTGTTGGGAGCATTAAACTTGATGGCAGTGAAATTGCCATTGGTGAGGTGCCGTTAACCTTCATCGTTGATGCAGATCAATGGCTGTTAc                               <  1:1607577/100‑1 (MQ=255)

                                                               ggAGGGATGATTGTTGGGAGCATTAAACTTGATGGCAGTGAAATTGCCATTGGTGAGGTGCCGTTAACCTTCATCGTTGATGCAGATCAATGGCTGTTAc                               >  2:2440113/1‑100 (MQ=255)

                                                                 aGGGATGATTGTTGGGAGCATTAAACTTGATGACAGTGAAATTGCCATTGGTGAGGTGCCGTTAACCTTCATCGTTGATGTAGATCAATGGCTGTTACAg                             <  2:2664208/100‑1 (MQ=255)

                                                                  gggATGATTGTTGGGAGCATTAAACTTGATGGCAGTGAAATTGCCATTGGTGAGGTGCCGTTAACCTTCATCGTTGATGCAGATCAATGGCTGTTACAgg                            <  1:2571137/100‑1 (MQ=255)

                                                                  gggATGATTGTTGGGAGCATTAAACTTGATGGCAGTGAAATTGCCATTGGTGAGGTGCCGTTAACCTTCATCGTTGATGCAGATCAATGGCTGTTACAgg                            <  2:2414880/100‑1 (MQ=255)

                                                                  gggATGATTGTTGGGAGCATTAAACTTGATGGCAGTGAAATTGCCATTGGTGAGGTGCCGTTAACCTTCATCGTTGATGCAGATCAATGGCTGTTACAgg                            >  2:138628/1‑100 (MQ=255)

                                                                  gggATGATTGTTGGGAGCATTAAACTTGATGGCAGTGAAATTGCCATTGGTGAGGTGCCGTTAACCTTCATCGTTGATGCAGATCAATGGCTGTTACAgg                            >  2:1245139/1‑100 (MQ=255)

                                                                   ggATGATTGTTGGGAGCATTAAACTTGATGGCAGTGAAATTGCCATTGGTGAGGTGCCGTTAACCTTCATCGTTGATGCAGATCAATGGCTGTTACAggg                           <  1:138626/100‑1 (MQ=255)

                                                                   ggATGATTGTTGGGAGCATTAAACTTGATGGCAGTGAAATTGCCATTGGTGAGGTGCCGTTAACCTTCATCGTTGATGCAGATCAATGGCTGTTACAggg                           >  2:874810/1‑100 (MQ=255)

                                                                    gatgatTGTTGGGAGCATTAAACTTGATGGCAGTGAAATTGCCATTGGTGAGGTGCCGTTAACCTTCATCGTTGATGCAGATCAATGGCTGTTACAGGGa                          >  2:1590784/1‑100 (MQ=255)

                                                                      tgatTGTTGGGAGCATTAAACTTGATGGCAGTGAAATTGCCATTGGTGAGGTGCCGTTAACCTTCATCGTTGATGCAGATCAATGGCTGTTACAGGGACa                        >  1:2387938/1‑100 (MQ=255)

                                                                       gatTGTTGGGAGCATTAAACTTGATGGCAGTGAAATTGCCATTGGTGAGGTGCCGTTAACCTTCATCGTTGATGCAGATCAATGGCTGTTACAGGGACAg                       >  2:815418/1‑100 (MQ=255)

                                                                       gatTGTTGGGAGCATTAAACTTGATGGCAGTGAAATTGCCATTGGTGAGGTGCCGTTAACCTTCATCGTTGATGCAGATCAATGGCTGTTACAGGGACAg                       <  2:1160340/100‑1 (MQ=255)

                                                                       gatTGTTGGGAGCATTAAACTTGATGGCAGTGAAATTGCCATTGGTGAGGTGCCGTTAACCTTCATCGTTGATGCAGATCAATGGCTGTTACAGGGACAg                       >  2:1083078/1‑100 (MQ=255)

                                                                        atTGTTGGGAGCATTAAACTTGATGGCAGTGAAATTGCCATTGGTGAGGTGCCGTTAACCTTCATCGTTGATGCAGATCAATGGCTGTTACAGGGACAgg                      >  1:2104398/1‑100 (MQ=255)

                                                                         ttgttgGGAGCATTAAACTTGATGGCAGTGAAATTGCCATTGGTGAGGTGCCGTTAACCTTCATCGTTGATGCAGATCAATGGCTGTTACAGGGACAGGc                     <  1:95818/100‑1 (MQ=255)

                                                                          tgttgGGAGCATTAAACTTGATGGCAGTGAAATTGCCATTGGTGAGGTGCCTTTAACCTTCATCGTTGATGCAGATCAATGGCTGTTACAGGGACAGGct                    >  1:1314366/1‑100 (MQ=255)

                                                                          tgttgGGAGCATTAAACTTGATGGCAGTGAAATTGCCATTGGTGAGGTGCCGTTAACCTTCATCGTTGATGCAGATCAATGGCTGTTACAGGGACAGGct                    <  1:1637111/100‑1 (MQ=255)

                                                                           gttgGGAGCATTAAACTTGATGGCAGTGAAATTGCCATTGGTGAGGTGCCGTTAACCTTCATCGTTGATGCAGATCAATGGCTGTTACAGGGACAGGctt                   >  2:279631/1‑100 (MQ=255)

                                                                                gAGCATTAAACTTGATGGCAGTGAAATTGCCATTGGTGAGGTGCCGTTAACCTTCATCGTTGATGCAGATCAATGGCTGTTACAGGGACAGGCTTCTTGc              >  1:1591052/1‑100 (MQ=255)

                                                                                  gCATTAAACTTGATGGCAGTGAAATTGCCATTGGTGAGGTGCCGTTAACCTTCATCGTTGATGCAGATCAATGGCTGGTACAGGGACAGGCTTCTTGCAg            <  1:1638797/100‑1 (MQ=255)

                                                                                   cATTAAACTTGATGGCAGTGAAATTGCCATTGGTGAGGTGCCGTTAACCTTCATCGTTGATGCAGATCAATGGCTGTTACAGGTACAGGCTTCTTGCAgt           >  1:2130101/1‑100 (MQ=255)

                                                                                     ttAAACTTGATGGCAGTGAAATTGCCATTGGTGAGGTGCCGTTAACCTTCATCGTTGATGCAGATCAATGGCTGTTACAGGGACAGGCTTCTTGCAgtgt         <  1:2025057/100‑1 (MQ=255)

                                                                                     ttAAACTTGATGGCAGTGAAATTGCCATTGGTGAGGTGCCGTTAACCTTCATCGTTGATGCAGATCAATGGCTGTTACAGGGACAGGCTTCTTGCAgtgt         <  1:948092/100‑1 (MQ=255)

                                                                                      tAAACTTGATGGCAGTGAAATTGCCATTGGTGAGGTGCCGTTAACCTTCATCGTTGATGCAGATCAATGGCTGTTACAGGGACAGGCTTCTTGCAGtgtg        <  2:1505506/100‑1 (MQ=255)

                                                                                      tAAACTTGATGGCAGTGAAATTGCCATTGGTGAGGTGCCGTTAACCTTCATCGTTGATGCAGATCAATGGCTGTTACAGGGACAGGCTTCTTGCAGtgtg        <  2:2199264/100‑1 (MQ=255)

                                                                                       aaaCTTGATGGCAGTGAAATTGCCATTGGTGAGGTGCCGTTAACCTTCATCGTTGATGCAGATCAATGGCTGTTACAGGGACAGGCTTCTTGCAGTGTGc       <  1:1454514/100‑1 (MQ=255)

                                                                                         aCTTGATGGCAGTGAAATTGCCATTGGTGAGGTGCCGTTAACCTTCATCGTTGATGCAGATCAATGGCTGTTACAGGGACAGGCTTCTTGCAGTGTGCGa     >  2:2789849/1‑100 (MQ=255)

                                                                                         aCTTGATGGCAGTGAAATTGCCATTGGTGAGGTGCCGTTAACCTTCATCGTTGATGCAGATCAATGGCTGTTACAGGGACAGGCTTCTTGCAGTGTGCGa     >  2:876935/1‑100 (MQ=255)

                                                                                          cTTGATGGCAGTGAAATTGCCATTGGTGAGGTGCCGTTAACCTTCATCGTTGATGCAGATCAATGGCTGTTACAGGGACAGGCTTCTTGCAGTGTGCGaa    <  2:2377995/100‑1 (MQ=255)

                                                                                          cTTGATGGCAGTGAAATTGCCATTGGTGAGGTGCCGTTAACCTTCATCGTTGATGCAGATCAATGGCTGTTACAGGGACAGGCTTCTTGCAGTGTGCGaa    <  1:366626/100‑1 (MQ=255)

                                                                                            tgatggCAGTGAAATTGCCATTGGTGAGGTGCCGTTAACCTTCATCGTTGATGCAGATCAATGGCTGTTACAGGGACAGGCTTCTTGCAGTGTGCGAagc  <  1:1483432/100‑1 (MQ=255)

AAAAGCGAAGTGAGATTTGGCAGGCAAAATCCATCGGCAGGTTTGTCGTTAGTGGCTCGTGCTGGAGGGATGATTGTTGGGAGCATTAAACTTGATGACAGTGAAATTGCCATTGGTGAGGTGCCGTTAACCTTCATCGTTGATGCAGATCAATGGCTGTTACAGGGACAGGCTTCTTGCAGTGTGCGAAGC  >  NC_000913/4573431‑4573622

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 22 ≤` `ATCG/ATCG` `< 32 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A