Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A3 F110 I0 R1	60	98.0	4713732	96.7%	4558178	99.9

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_000913	4,573,528	A→G	100%	pseudogene (1115/1503 nt)	yjiT →	pseudogene

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,573,528	0	A	G	97.3%	384.7 / ‑0.3	112	pseudogene (1115/1503 nt)	yjiT	pseudogene
Reads supporting (aligned to +/- strand): ref base A (1/2); new base G (48/60); total (49/63)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.18e-01

Only 100 of 115 total aligned reads displayed.

AAAGCGAAGTGAGATTTGGCAGGCAAAATCCATCGGCAGGTTTGTCGTTAGTGGCTCGTGCTGGAGGGATGATTGTTGGGAGCATTAAACTTGATGACAGTGAAATTGCCATTGGTGAGGTGCCGTTAACCTTCATCGTTGATGCAGATCAATGGCTGTTACAGGGACAGGCTTCTTGCAGTGTGCGAAGCA  >  NC_000913/4573432‑4573623

aaaGCGAAGTGAGATTTGGCAGGCAAAATCCATCGGCAGGTTTGTCGTTAGTGGCTCGTGCTGGAGGGATGATTGTTGGGAGCATTAAACTTGATGGCAg                                                                                              >  1:1859362/1‑100 (MQ=255)

aaaGCGAAGTGAGATTTGGCAGGCAAAATCCATCGGCAGGTTTGTCGTTAGTGGCTCGTGCTGGAGGGATGATTGTTGGGAGCATTAAACTTGATGGCAg                                                                                              <  1:131360/100‑1 (MQ=255)

aaaGCGAAGTGAGATTTGGCAGGCAAAATCCATCGGCAGGTTTGTCGTTAGTGGCTCGTGCTGGAGGGATGATTGTTGGGAGCATTAAACTTGATGGCAg                                                                                              <  2:134119/100‑1 (MQ=255)

 aaGCGAAGTGAGATTTGGCAGGCAAAATCCATCGGCAGGTTTGTCGTTAGTGGCTCGTGCTGGAGGGATGATTGTTGGGAGCATTAAACTTGATGGCAg                                                                                              <  1:661517/99‑1 (MQ=255)

  aGCGAAGTGAGATTTGGCAGGCAAAATCCATCGGCAGGTTTGTCGTTAGTGGCTCGTGCTGGAGGGCTGCTTGTTGGGAGCATTAAACTTGATGGCAGTg                                                                                            >  2:882234/1‑100 (MQ=255)

    cGAAGTGAGATTTGGCAGGCAAAATCCATCGGCAGGTTTGTCGTTAGTGGCTCGTGCTGGAGGGATGATTGTTGGGAGCATTAAACTTGATGACAGTGaa                                                                                          <  1:2136214/100‑1 (MQ=255)

     gAAGTGAGATTTGGCAGGCAAAATCCATCGGCAGGTTTGTCGTTAGTGGCTCGTGCTGGAGGGATGATTGTTGGGAGCATTAAACTTGATGGCAGTGaaa                                                                                         >  1:1974976/1‑100 (MQ=255)

      aaGTGAGATTTGGCAGGCAAAATCCATCGGCAGGTTTGTCGTTAGTGGCTCGTGCTGGAGGGATGATTGTTGGGAGCATTAAACTTGATGGCAGTGAAAt                                                                                        >  1:935394/1‑100 (MQ=255)

      aaGTGAGATTTGGCAGGCAAAATCCATCGGCAGGTTTGTCGTTAGTGGCTCGTGCTGGAGGGATGATTGTTGGGAGCATTAAACTTGATGGCAGTGAAAt                                                                                        >  1:717217/1‑100 (MQ=255)

      aaGTGAGATTTGGCAGGCAAAATCCATCGGCAGGTTTGTCGTTAGTGGCTCGTGCTGGAGGGATGATTGTTGGGAGCATTAAACTTGATGGCAGTGAAAt                                                                                        >  2:2055687/1‑100 (MQ=255)

        gTGAGATTTGGCAGGCAAAATCCATCGGCAGGTTTGTCGTTAGTGGCTCGTGCTGGAGGGATGATTGTTGGGAGCATTAAACTTGATGGCAGTGAAATTg                                                                                      >  2:2694/1‑100 (MQ=255)

        gTGAGATTTGGCAGGCAAAATCCATCGGCAGGTTTGTCGTTAGTGGCTCGTGCTGGAGGGATGATTGTTGGGAGCATTAAACTTGATGGCAGTGAAATTg                                                                                      <  1:1663918/100‑1 (MQ=255)

            gaTTTGGCAGGCAAAATCCATCGGCAGGTTTGTCGTTAGTGGCTCGTGCTGGAGGGATGATTGTTGGGAGCATTAAACTTGATGGCAGTGAAATTGCCAt                                                                                  >  2:83106/1‑100 (MQ=255)

            gaTTTGGCAGGCAAAATCCATCGGCAGGTTTGTCGTTAGTGGCTCGTGCTGGAGGGATGATTGTTGGGAGCATTAAACTTGATGACAGTGAAATTGCCAt                                                                                  >  1:1618880/1‑100 (MQ=255)

             aTTTGGCAGGCAAAATCCATCGGCAGGTTTGTCGTTAGTGGCTCGTGCTGGAGGGATGATTGTTGGGAGCATTAAACTTGATGGCAGTGAAATTGCCAtt                                                                                 <  2:390229/100‑1 (MQ=255)

               ttGGCAGGCAAAATCCATCGGCAGGTTTGTCGTTAGTGGCTCGTGCTGGAGGGATGATTGTTGGGAGCATTAAACTTGATGGCAGTGAAATTGCCATTgg                                                                               <  2:1865542/100‑1 (MQ=255)

                 ggcaggcaAAATCCATCGGCAGGTTTGTCGTTAGTGGCTCGTGCTGGAGGGATGATTGTTGGGAGCATTAAACTTGATGGCAGTGAAATTGCCATTGGTg                                                                             >  2:2023130/1‑100 (MQ=255)

                 ggcaggcaAAATCCATCGGCAGGTTTGTCGTTAGTGGCTCGTGCTGGAGGGATGATTGTTGGGAGCATTAAACTTGATGGCAGTGAAATTGCCATTGGTg                                                                             <  1:1987271/100‑1 (MQ=255)

                   caggcaAAATCCATCGGCAGGTTTGTCGTTAGTGGCTCGTGCTGGAGGGATGATTGTTGGGAGCATTAAACTTGATGGCAGTGAAATTGCCATTTGTGAg                                                                           <  2:1803426/100‑1 (MQ=255)

                    aggcaAAATCCATCGGCAGGTTTGTCGTTAGTGGCTCGTGCTGGAGGGATGATTGTTGGGAGCATTAAACTTGATGGCAGTGAAATTGCCATTGGTGAgg                                                                          <  1:453623/100‑1 (MQ=255)

                     ggcaAAATCCATCGGCAGGTTTGTCGTTAGTGGCTCGTGCTGGAGGGATGATTGTTGGGAGCCTTAAACTTGATGGCAGTGAAATTGCCATTGGTGAGGt                                                                         >  1:811887/1‑100 (MQ=255)

                     ggcaAAATCCATCGGCAGGTTTGTCGTTAGTGGCTCGTGCTGGAGGGATGATTGTTGGGAGCATTAAACTTGATGGCAGTGAAATTGCCATTGGTGAGGt                                                                         >  1:813285/1‑100 (MQ=255)

                     ggcaAAATCCATCGGCAGGTTTGTCGTTAGTGGCTCGTGCTGGAGGGATGATTGTTGGGAGCATTAAACTTGATGGCAGTGAAATTGCCATTGGTGAGGt                                                                         >  1:2199549/1‑100 (MQ=255)

                      gcaAAATCCATCGGCAGGTTTGTCGTTAGTGGCTCGTGCTGGAGGGATGATTGTTGGGAGCATTAAACTTGATGGCAGTGAAATTGCCATTGGTGAGGTg                                                                        >  2:864013/1‑100 (MQ=255)

                      gcaAAATCCATCGGCAGGTTTGTCGTTAGTGGCTCGTGCTGGAGGGATGATTGTTGGGAGAATTAAACTTGATGGCAGTGAAATTGCCATTGGTGAGGTg                                                                        >  2:2149987/1‑100 (MQ=255)

                          aaTCCATCGGCAGGTTTGTCGTTAGTGGCTCGTGCTGGAGGGATGATTGTTGGGAGCATTAAACTTGATGGCAGTGAAATTGCCATTGGTGAGGTGCCGt                                                                    <  1:1298445/100‑1 (MQ=255)

                          aaTCCATCGGCAGGTTTGTCGTTAGTGGCTCGTGCTGGAGGGATGATTGTTGGGAGCATTAAACTTGATGGCAGTGAAATTGCCATTGGTGAGGTGCCGt                                                                    <  2:2193677/100‑1 (MQ=255)

                            tCCATCGGCAGGTTTGTCGTTAGTGGCTCGTGCTGGAGGGATGATTGTTGGGAGCATTAAACTTGATGGCAGTGAAATTGCCATTGGTGAGGTGCCGTTa                                                                  >  1:1879776/1‑100 (MQ=255)

                              cATCGGCAGGTTTGTCGTTAGTGGCTCGTGCTGGAGGGATGATTGTTGGGAGCATTAAACTTGATGGCAGTGAAATTGCCATTGGTGAGGTGCCGTTAAc                                                                >  2:242189/1‑100 (MQ=255)

                              cATCGGCAGGTTTGTCGTTAGTGGCTCGTGCTGGAGGGATGATTGTTGGGAGCATTAAACTTGATGGCAGTGAAATTGCCATTGGTGAGGTGCCGTTAAc                                                                >  1:1402450/1‑100 (MQ=255)

                               aTCGGCAGGTTTGTCGTTAGTGGCTCGTGCTGGAGGGATGATTGTTGGGAGCATTAAACTTGATGGCAGTGAAATTGCCATTGGTGAGGTGCCGTTAAcc                                                               <  2:575443/100‑1 (MQ=255)

                                 cGGCAGGTTTGTCGTTAGTGGCTCGTGCTGGAGGGATGATTGTTGGGAGCATTAAACTTGATGGCAGTGAAATTGCCATTGGTGAGGTGCCGTTAACCtt                                                             <  2:1785736/100‑1 (MQ=255)

                                  ggCAGGTTTGTCGTTAGTGGCTCGTGCTGGAGGGATGATTGTTGGGAGCATTAAACTTGATGGCAGTGAAATTGCCATTGGTGAGGTGCCGTTAACCTTc                                                            >  2:11999/1‑100 (MQ=255)

                                  ggCAGGTTTGTCGTTAGTGGCTCGTGCTGGAGGGATGATTGTTGGGAGCATTAAACTTGATGGCAGTGAAATTGCCATTGGTGAGGTGCCGTTAACCTTc                                                            >  1:1771839/1‑100 (MQ=255)

                                    cAGGTTTGTCGTTAGTGGCTCGTGCTGGAGGGATGATTGTTGGGTGCATTAAACTTGATGGCAGTGAAATTGCCATTGGTGAGGTGCCGTTAACCTTCAt                                                          <  2:168242/100‑1 (MQ=255)

                                     aGGTTTGTCGTTAGTGGCTCGTGCTGGAGGGATGATTGTTGGGAGCATTAAACTTGATGGCAGTGAAATTGCCATTGGTGAGGTGCCGTTAACCTTCATc                                                         <  2:69422/100‑1 (MQ=255)

                                      ggTTTGTCGTTAGTGGCTCGTGCTGGAGGGATGTTTGTTGGGAGCATTAATCTTGATGTCAGTGAAATTGCCATTGGTGCGGTGCCGTTAACCTTCATCg                                                        <  2:192441/100‑1 (MQ=255)

                                      ggTTTGTCGTTAGTGGCTCGTGCTGGAGGGATGATTGTTGGGAGCATTAAACTTGATGGCAGTGAAATTGCCATTGGTGAGGTGCCGTTAACCTTCATCg                                                        >  1:1026516/1‑100 (MQ=255)

                                      ggTTTGTCGTTAGTGGCTCGTGCTGGAGGGATGATTGTTGGGAGCATTAAACTTGATGGCAGTGAAATTGCCATTGGTGAGGTGCCGTTAACCTTCATCg                                                        >  1:208032/1‑100 (MQ=255)

                                      ggTTTGTCGTTAGTGGCTCGTGCTGGAGGGATGATTGTTGGGAGCATTAAACTTGATGACAGTGAAATTGCCATTGGTGAGGTGCCGTTAACCTTCATCg                                                        <  2:1618869/100‑1 (MQ=255)

                                         ttGTCGTTAGTGGCTCGTGCTGGAGGGATGATTGTTGGGAGCATTAAACTTGATGGCAGTGAAATTGCCATTGGTGAGGTGCCGTTAACCTTCATCGTTg                                                     <  1:2024149/100‑1 (MQ=255)

                                         ttGTCGTTAGTGGCTCGTGCTGGAGGGATGATTGTTGGGAGCATTAAACTTGATGGCAGTGAAATTGCCATTGGTGAGGTGCCGTTAACCTTCATCGTTg                                                     <  2:1304258/100‑1 (MQ=255)

                                          tGTCGTTAGTGGCTCGTGCTGGAGGGATGATTGTTGGGAGCATTAAACTTGATGGCAGTGAAATTGCCATTGGTGAGGTGCCGTTAACCTTCATCGTTGa                                                    <  2:170603/100‑1 (MQ=255)

                                           gTCGTTAGTGGCTCGTGCTGGAGGGATGATTGTTGGGAGCATTAAACTTGATGGCAGTGAAATTGCCATTGGTGAGGTGCCGTTAACCTTCATCGTTGAt                                                   <  1:2249649/100‑1 (MQ=255)

                                           gTCGTTAGTGGCTCGTGCTGGAGGGATGATTGTTGGGAGCATTAAACTTGATGGCAGTGAAATTGCCATTGGTGAGGTGCCGTTAACCTTCATCGTTGAt                                                   <  1:47952/100‑1 (MQ=255)

                                            tCGTTAGTGGCTCGTGCTGGAGGGATGATTGTTGGGAGCATTAAACTTGATGGCAGTGAAATTGCCATTGGTGAGGTGCCGTTAACCTTCATCGTTGATg                                                  <  1:1114791/100‑1 (MQ=255)

                                             cGTTAGTGGCTCGTGCTGGAGGGATGATTGTTGGGAGCATTAAACTTGATGGCAGTGAAATTGCCATTGGTGAGGTGCCGTTAACCTTCATCGTTGATGc                                                 <  1:1225268/100‑1 (MQ=255)

                                             cGTTAGTGGCTCGTGCTGGAGGGATGATTGTTGGGAGCATTAAACTTGATGGCAGTGAAATTGCCATTGGTGAGGTGCCGTTAACCTTCATCGTTGATGc                                                 >  1:1221417/1‑100 (MQ=255)

                                             cGTTAGTGGCTCGTGCTGGAGGGATGATTGTTGGGAGCATTAAACTTGATGGCAGTGAAATTGCCATTGGTGAGGTGCCGTTAACCTTCATCGTTGATGc                                                 >  1:1510263/1‑100 (MQ=255)

                                              gTTAGTGGCTCGTGCTGGAGGGATGATTGTTGGGAGCATGAAACTTGATGGCAGTGAAATTGCCATTGGTGAGGTGCCGTTAACCTTCATCGTTGATGCa                                                >  2:20845/1‑100 (MQ=255)

                                               ttAGTGGCTCGTGCTGGAGGGATGATTGTTGGGAGCATTAAACTTGATGGCAGTGAAATTGCCATTGGTGAGGTGCCGTTAACCTTCATCGTTGATGCAg                                               <  2:607493/100‑1 (MQ=255)

                                                 aGTGGCTCGTGCTGGAGGGATGATTGTTGGGAGCATTAAACTTGATGGCAGTGAAATTGCCATTGGTGAGGTGCCGTTAACCTTCATCGTTGATGCAGAt                                             >  2:272069/1‑100 (MQ=255)

                                                  gTGGCTCGTGCTGGAGGGATGATTGTTGGGAGCATTAAACTTGATGGCAGTGAAATTGCCATTGGTGAGGTGCCGTTAACCTTCATCGTTGATGCAGATc                                            <  1:1426943/100‑1 (MQ=255)

                                                    ggCTCGTGCTGGAGGGATGATTGTTGGGAGCATTAAACTTGATGGCAGTGAAATTGCCATTGGTGAGGTGCCGTTAACCTTCATCGTTGATGCAGATCaa                                          <  1:1203074/100‑1 (MQ=255)

                                                    ggCTCGTGCTGGAGGGATGATTGTTGGGAGCATTAAACTTGATGGCAGTGAAATTGCCATTGGTGAGGTGCCGTTAACCTTCATCGTTGATGCAGATCaa                                          >  1:1225352/1‑100 (MQ=255)

                                                      cTCGTGCTGGAGGTATGATTGTTCGGAGCATTAAACTTGATGGGAGTGAAATAGCCATTGGTGAGGTGCCGTTAACCTTCATCGTTGATGCAGATCAATg                                        <  2:604007/100‑1 (MQ=255)

                                                      cTCGTGCTGGAGGGATGATTGTTGGGAGCATTAAACTTGATGGCAGTGAAATTGCCATTGGTGAGGTGCCGTTAACCTTCATCGTTGATGCAGATCAATg                                        <  2:33833/100‑1 (MQ=255)

                                                      cTCGTGCTGGAGGGATGATTGTTGGGAGCATTAAACTTGATGGCAGTGAAATTGCCATTGGTGAGGTGCCGTTAACCTTCATCGTTGATGCAGATCAATg                                        >  1:1384793/1‑100 (MQ=255)

                                                       tCGTGCTGGAGGGATGATTGTTGGGAGCATTAAACTTGATGGCAGTGAAATTGCCATTGGTGAGGTGCCGTTAACCTTCATCGTTGATGCAGATCAATgg                                       >  1:421875/1‑100 (MQ=255)

                                                        cGTGCTGGAGGGATGATTGTTGGGAGCATTAAACTTGATGGCAGTGAAATTGCCATTGGTGAGGTGCCGTTAACCTTCATCGTTGATGCAGATCAATGGc                                      <  1:723032/100‑1 (MQ=255)

                                                        cGTGCTGGAGGGATGATTGTTGGGAGCATTAAACTTGATGGCAGTGAAATTGCCATTGGTGAGGTGCCGTTAACCTTCATCGTTGATGCAGATCAATGGc                                      >  1:1843820/1‑100 (MQ=255)

                                                        cGTGCTGGAGGGATGATTGTTGGGAGCATTAAACTTGATGGCAGTGAAATTGCCATTGGTGAGGTGCCGTTAACCTTCATCGTTGATGCAGATCAATGGc                                      <  2:479060/100‑1 (MQ=255)

                                                         gTGCTGGAGGGATGATTGTTGGGAGCATTAAACTTGATGGCAGTGAAATTGCCATTGGTGAGGTGCCGTTAACCTTCATCGTTGATGCAGATCAATGGCt                                     >  1:1736645/1‑100 (MQ=255)

                                                          tGCTGGAGGGATGATTGTTGGGAGCATTAAACTTGATGGCAGTGAAATTGCCATTGGTGAGGTGCCGTTAACCTTCATCGTTGATGCAGATCAATGGCTg                                    <  1:2011060/100‑1 (MQ=255)

                                                            cTGGAGGGATGATTGTTGGGAGCATTAAACTTGATGGCAGTGAAATTGCCATTGGTGAGGTGCCGTTAACCTTCATCGTTGATGCAGATCAATGGCTGtt                                  >  1:484394/1‑100 (MQ=255)

                                                              ggAGGGATGATTGTTGGGAGCATTAAACTTGATGGCAGTGAAATTGCCATTGGTGAGGTGCCGTTAACCTTCATCGTTGATGCAGATCAATGGCTGTTAc                                >  1:2336642/1‑100 (MQ=255)

                                                               gAGGGATGATTGTTGGGAGCATTAAACTTGATGGCAGTGAAATTGCCATTGGTGAGGTGCCGTTAACCTTCATCGTTGATGCAGATCAATGGCTGTTACa                               <  1:1010299/100‑1 (MQ=255)

                                                               gAGGGATGATTGTTGGGAGCATTAAACTTGATGGCAGTGAAATTGCCATTGGTGAGGTGCCGTTAACCTTCATCGTTGATGCAGATCAATGGCTGTTACa                               <  1:83106/100‑1 (MQ=255)

                                                                aGGGATGATTGTTGGGAGCATTAAACTTGATGGCAGTGAAATTGCCATTGGTGAGGTGCCGTTAACCTTCATCGTTGATGCAGATCAATGGCTGTTACAg                              >  2:1129474/1‑100 (MQ=255)

                                                                  ggATGATTGTTGGGAGCATTAAACTTGATGGCAGTGAAATTGCCATTGGTGAGGTGCCGTTAACCTTCATCGTTGATGCAGATCAATGGCTGTTACAggg                            <  2:1592775/100‑1 (MQ=255)

                                                                    atgatTGTTGGGAGCATTAAACTTGATGGCAGTGAAATTGCCATTGGTGAGGTGCCGTTAACCTTCATCGTTGATGCAGATCAATGGCTGTTACAGGGAc                          <  1:1061274/100‑1 (MQ=255)

                                                                    atgatTGTTGGGAGCATTAAACTTGATGGCAGTGAAATTGCCATTGGTGAGGTGCCGTTAACCTTCATCGTTGATGCAGATCAATGGCTGTTACAGGGAc                          <  2:874647/100‑1 (MQ=255)

                                                                    atgatTGTTGGGAGCATTAAACTTGATGGCAGTGAAATTGCCATTGGTGAGGTGCCGTTAACCTTCATCGTTGATGCAGATCAATGGCTGTTACAGGGAc                          <  1:2012758/100‑1 (MQ=255)

                                                                     tgatTGTTGGGAGCATTAAACTTGATGGCAGTGAAATTGCCATTGGTGAGGTGCCGTTAACCTTCATCGTTGATGCAGATCAATGGCTGTTACAGGGACa                         >  2:439532/1‑100 (MQ=255)

                                                                     tgatTGTTGGGAGCATTAAACTTGATGGCAGTGAAATTGCCATTGGTGAGGTGCCGTTAACCTTCATCGTTGATGCAGATCAATGGCTGTTACAGGGACa                         <  2:2221766/100‑1 (MQ=255)

                                                                       atTGTTGGGAGCATTAAACTTGATGGCAGTGAAATTGCCATTGGTGAGGTGCCGTTAACCTTCATCGTTGATGCAGATCAATGGCTGTTACAGGGACAgg                       <  2:857219/100‑1 (MQ=255)

                                                                         tgttgGGAGCATTAAACTTGATGGCAGTGAAATTGCCATTGGTGAGGTGCCGTTAACCTTCATCGTTGATGCAGATCAGTGGCTGTTACAGGGACAGGct                     >  2:209717/1‑100 (MQ=255)

                                                                         tgttgGGAGCATTAAACTTGATGGCAGTGAAATTGCCATTGGTGAGGTGCCGTTAACCTTCATCGTTGATGCAGATCAATGGCTGTTACAGGGACAGGct                     <  2:1336598/100‑1 (MQ=255)

                                                                           ttgGGAGCATTAAACTTGATGGCAGTGAAATTGCCATTGGTGAGGTGCCGTTAACCTTCATCGTTGATGCAGATCAATGGCTGTTACAGGGACAGGcttc                   <  2:1402442/100‑1 (MQ=255)

                                                                             gggAGCATTAAACTTGATGGCAGTGAAATTGCCATTGGTGAGGTGCCGTTAACCTTCATCGTTGATGTAGATCAATGGCTGTTACAGGGACAGGcttctt                 <  2:1178654/100‑1 (MQ=255)

                                                                              ggAGCATTAAACTTGATGGCAGTGAAATTGCCATTGGTGAGGTGCCGTTAACCTTCATCGTTGATGCAGATCAATGGCTGTTACAGGGACAGGCTTCTTg                >  1:1321662/1‑100 (MQ=255)

                                                                              ggAGCATTAAACTTGATGGCAGTGAAATTGCCATTGGTGAGGTGCCGTTAACCTTCATCGTTGATGCAGATCAATGGCTGTTACAGGGACAGGCTTCTTg                <  1:56799/100‑1 (MQ=255)

                                                                               gAGCATTAAACTTGATGGCAGTGAAATTGCCATTGGTGAGGTGCCGTTAACCTTCATCGTTGATGCAGATCAATGGCTGTTACAGGGACAGGCTTCTTGc               <  2:668314/100‑1 (MQ=255)

                                                                               gAGCATTAAACTTGATGGCAGTGAAATTGCCATTGGTGAGGTGCCGTTAACCTTCATCGTTGATGCAGATCAATGGCTGTTACAGGGACAGGCTTCTTGc               <  2:24611/100‑1 (MQ=255)

                                                                               gAGCATTAAACTTGATGGCAGTGAAATTGCCATTGGTGAGGTGCCGTTAACCTTCATCGTTGATGCAGATCAATGGCTGTTACAGGGACAGGCTTCTTGc               <  1:1434884/100‑1 (MQ=255)

                                                                               gAGCATTAAACTTGATGGCAGTGAAATTGCCATTGGTGAGGTGCCGTTAACCTTCATCGTTGATGCAGATCAATGGCTGTTACAGGGACAGGCTTCTTGc               <  1:441947/100‑1 (MQ=255)

                                                                               gAGCAGTAAACTTGATGGCAGTGATATTGCCATTGGTGAGGTGCCGTTAACCTTCATCGTTGATGCAGATCAATGGCTGTTACAGGGACAGGCTTCTTGc               <  2:101658/100‑1 (MQ=255)

                                                                                 gCATTAAACTTGATGGCAGTGAAATTGCCATTGGTGAGGTGCCGTTAACCTTCATCGTTGATGCAGATCAATGGCTGTTACAGGGACAGGCTTCTTGCAg             <  1:1542854/100‑1 (MQ=255)

                                                                                 gCATTAAACTTGATGGCAGTGAAATTGCCATTGGTGAGGTGCCGTTAACCTTCATCGTTGATGCAGATCAATGGCTGTTACAGGGACAGGCTTCTTGCAg             <  1:1662542/100‑1 (MQ=255)

                                                                                  cATTAAACTTGATGGCAGTGAAATTGCCATTGGTGAGGTGCCGTTAACCTTCATCGTTGATGCAGATCAATGGCTGTTACAGGGACAGGCTTCTTGCAgt            >  2:2232375/1‑100 (MQ=255)

                                                                                   aTTAAACTTGATGGCAGTGAAATTGCCATTGGTGAGGTGCCGTTAACCTTCATCGTTGATGCAGATCAATGGCTGTTACAGGGACAGGCTTCTTGCAgtg           <  1:1700805/100‑1 (MQ=255)

                                                                                    ttAAACTTGATGGCAGTGAAATTGCCATTGGTGAGGTGCCGTTAACCTTCATCGTTGATGCAGATCAATGGCTGTTACAGGGACAGGCTTCTTGCAgtgt          >  1:131763/1‑100 (MQ=255)

                                                                                    ttAAACTTGATGGCAGTGAAATTGCCATTGGTGAGGTGCCGTTAACCTTCATCGTTGATGCAGATCAATGGCTGTTACAGGGACAGGCTTCTTGCAgtgt          >  1:1003634/1‑100 (MQ=255)

                                                                                     tAAACTTGATGGCAGTGAAATTGCCATTGGTGAGGTGCCGTTAACCTTCATCGTTGATGCAGATCAATGGCTGTTACAGGGACAGGCTTCTTGCAGtgtg         <  1:1805881/100‑1 (MQ=255)

                                                                                     tAAACTTGATGGCAGTGAAATTGCCATTGGTGAGGTGCCGTTAACCTTCATCGTTGATGCAGATCAATGGCTGTTACAGGGACAGGCTTCTTGCAGtgtg         <  2:1221412/100‑1 (MQ=255)

                                                                                      cgatctgatggCAGTGAAATTGCCATTGGTGAGGTGCCGTTAACCTTCATCGTTGATGCAGATCAATGGCTGTTACAGGGACAGGCTTCTTGCAGTGTGc        <  1:726867/95‑1 (MQ=255)

                                                                                        aCTTGATGGCAGTGAAATTGCCATTGGTGAGGTGCCGTTAACCTTCATCGTTGATGCAGATCAATGGCTGTTACAGGGACAGGCTTCTTGCAGTGTGCGa      <  2:800584/100‑1 (MQ=255)

                                                                                        aCTTGATGGCAGTGAAATTGCCATTGGTGAGGTGCCGTTAACCTTCATCGTTGATGCAGATCAATGGCTGTTACAGGGACAGGCTTCTTGCAGTGTGCGa      <  2:1038056/100‑1 (MQ=255)

                                                                                           tgatggCAGTGAAATTGCCATTGGTGAGGTGCCGTTAACCTTCATCGTTGATGCAGATCAATGGCTGTTACAGGGACAGGCTTCTTGCAGTGTGCGAagc   <  1:1179611/100‑1 (MQ=255)

                                                                                            gatggCAGTGAAATTGCCATTGGTGAGGTGCCGTTAACCTTCATCGTTGATGCAGATCAATGGCTGTTACAGGGACAGGCTTCTTGCAGTGTGcagatcg  >  2:726864/1‑94 (MQ=255)

AAAGCGAAGTGAGATTTGGCAGGCAAAATCCATCGGCAGGTTTGTCGTTAGTGGCTCGTGCTGGAGGGATGATTGTTGGGAGCATTAAACTTGATGACAGTGAAATTGCCATTGGTGAGGTGCCGTTAACCTTCATCGTTGATGCAGATCAATGGCTGTTACAGGGACAGGCTTCTTGCAGTGTGCGAAGCA  >  NC_000913/4573432‑4573623

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 22 ≤` `ATCG/ATCG` `< 32 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A