Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A16 F201 I0 R1 306 176.9 8831342 93.4% 8248473 99.8

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 4,573,528 A→G 100% pseudogene (1115/1503 nt) yjiT → pseudogene

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,573,5280AG98.9% 613.7 / ‑3.7 175pseudogene (1115/1503 nt)yjiTpseudogene
Reads supporting (aligned to +/- strand):  ref base A (1/1);  new base G (76/97);  total (77/98)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.15e-01

Only 100 of 177 total aligned reads displayed.

GCGAAGTGAGATTTGGCAGGCAAAATCCATCGGCAGGTTTGTCGTTAGTGGCTCGTGCTGGAGGGATGATTGTTGGGAGCATTAAACTTGATGACAGTGAAATTGCCATTGGTGAGGTGCCGTTAACCTTCATCGTTGATGCAGATCAATGGCTGTTACAGGGACAGGCTTCTTGCAGTGTGCGAAGCAG  >  NC_000913/4573435‑4573624
                                                                                             |                                                                                                
gCGAAGTGAGATTTGGCAGGCAAAATCCATCGGCAGGTTTGTCGTTAGTGGCTCGTGATGGAGGGATGATTGTTGGGAGCATTAAACTTGATGGCtgtg                                                                                             >  1:1624880/1‑99 (MQ=255)
 cGAAGTGAGATTTGGCAGGCAAAATACATCGGCAGGTTTGTCGTTAGTGGCTCGTGCTGGAGGGATGATTGTTGGGAGCATTAAACTTGATGGCAGTGaa                                                                                           >  1:1340770/1‑100 (MQ=255)
  gAAGTGAGATTTGGCAGGCAAAATCCATCGGCAGGTTTGTCGTTAGTGGCTCGTGCTGGAGGGATGATTGTTGGGAGCATTAAACTTGATGGCAGTGaaa                                                                                          >  2:2333599/1‑100 (MQ=255)
     gTGAGATTTGGCAGGCAAAATCCATCGGCAGGTTTGTCGTTAGTGGCTCGTGCTGGAGGGATGATTGTTGGGAGCATTAAACTTGATGGCAGTGAAATTg                                                                                       <  1:1376925/100‑1 (MQ=255)
     gTGAGATTTGGCAGGCAAAATCCATCGGCAGGTTTGTCGTTAGTGGCTCGTGCTGGAGGGATGATTGTTGGGAGCATTAAACTTGATGGCAGTGAAATTg                                                                                       <  1:1710593/100‑1 (MQ=255)
     gTGAGATTTGGCAGGCAAAATCCATCGGCAGGTTTGTCGTTAGTGGCTCGTGCTGGAGGGATGATTGTTGGGAGCATTAAACTTGATGGCAGTGAAATTg                                                                                       <  1:3528504/100‑1 (MQ=255)
      tGAGATTTGGCAGGCAAAATCCATCGGCAGGTTTGTCGTTAGTGGCTCGTGCTGGAGGGATGATTGTTGGGAGCATTAAACTTGATGGCAGTGAAATTGc                                                                                      <  2:3173199/100‑1 (MQ=255)
       gagaTTTGGCAGGCAAAATCCATCGGCAGGTTTGTCGTTAGTGGCTCGTGCTGGAGGGATGATTGTTGGGAGCATTAAACTTGATGGCAGTGAAATTGcc                                                                                     <  1:1522581/100‑1 (MQ=255)
       gagaTTTGGCAGGCAAAATCCATCGGCAGGTTTGTCGTTAGTGGCTCGTGCTGGAGGGATGATTGTTGGGAGCATTAAACTTGATGGCAGTGAAATTGcc                                                                                     <  1:948361/100‑1 (MQ=255)
        agaTTTGGCAGGCAAAATCCATCGGCAGGTTTGTCGTTAGTGGCTCGTGCTGGAGGGATGATTGTTGGGAGCATTAAACTTGATGGCAGTGAAATTGCCa                                                                                    >  1:704813/1‑100 (MQ=255)
         gaTTTGGCAGGCAAAATCCATCGGCAGGTTTGTCGTTAGTGGCTCGTGCTGGAGGGATGATTGTTGGGAGCATTAAACTTGATGGCAGTGAAATTGCCAt                                                                                   >  1:1106946/1‑100 (MQ=255)
         gaTTTGGCAGGCAAAATCCATCGGCAGGTTTGTCGTTAGTGGCTCGTGCTGGAGGGATGATTGTTGGGAGCATTAAACTTGATGGCAGTGAAATTGCCAt                                                                                   >  1:3940243/1‑100 (MQ=255)
         gaTTTGGCAGGCAAAATCCATCGGCAGGTTTGTCGTTAGTGGCTCGTGCTGGAGGGATGATTGTTGGGAGCATTAAACTTGATGGCAGTGAAATTGCCAt                                                                                   >  1:3269885/1‑100 (MQ=255)
         gaTTTGGCAGGCAAAATCCATCGGCAGGTTTGTCGTTAGTGGCTCGTGCTGGAGGGATGATTGTTGGGAGCATTAAACTTGATGGCAGTGAAATTGCCAt                                                                                   >  1:3937954/1‑100 (MQ=255)
          aTTTGGCAGGCAAAATCCATCGGCAGGTTTGTCGTTAGTGGCTCGTGCTGGAGGGATGATTGTTGGGAGCATTAAACTTGATGGCAGTGAAATTGCCAtt                                                                                  <  2:2611392/100‑1 (MQ=255)
            ttGGCAGGCAAAATCCATCGGCAGGTTTGTCGTTAGTGGCTCGTGCTGGAGGGATGATTGTTGGGAGCATTAAACTTGATGGCAGTGAAATTGCCATTgg                                                                                <  2:2827122/100‑1 (MQ=255)
              ggcaggcaAAATCCATCGGCAGGTTTGTCGTTAGTGGCTCGTGCTGGAGGGATGATTGTTGGGAGCATTAAACTTGATGGCAGTGAAATTGCCATTGGTg                                                                              <  2:478859/100‑1 (MQ=255)
              ggcaggcaAAATCCATCGGCAGGTTTGTCGTTAGTGGCTCGTGCTGGAGGGATGATTGTTGGGAGCATTAAACTTGATGGCAGTGAAATTGCCATTGGTg                                                                              <  2:3520504/100‑1 (MQ=255)
                caggcaAAATCCATCGGCAGGTTTGTCGTTAGTGGCTCGTGCTGGAGGGATGATTGTTGGGAGCATTAAACTTGATGGCAGTGAAATTGCCATTGGTGAg                                                                            >  2:342283/1‑100 (MQ=255)
                 aggcaAAATCCATCGGCAGGTTTGTCGTTAGTGGCTCGTGCTGGAGGGATGATTGTTGGGAGCATTAAACTTGATGGCAGTGAAATTGCCATTGGTGAgg                                                                           <  1:3551999/100‑1 (MQ=255)
                 aggcaAAATCCATCGGCAGGTTTGTCGTTAGTGGCTCGTGCTGGAGGGATGATTGTTGGGAGCATTAAACTTGATGGCAGTGAAATTGCCATTGGTGAgg                                                                           <  1:2392557/100‑1 (MQ=255)
                   gcaAAATCCATCGGCAGGTTTGTCGTTAGTGGCTCGTCCTGTAGGGCTGGTTGTTGGGTGTTTTAATCTTGTTGGCAGTGAAATTGCCATtggtgtggtg                                                                         >  1:3021413/1‑100 (MQ=255)
                    caAAATCCATCGGCAGGTTTGTCGTTAGTGGCTCGTGCTGGAGGGATGATTGTTGGGAGCATTAACCTTGATGGCAGTGAAATTGCCATTGGTGAGGTGc                                                                        >  2:4023632/1‑100 (MQ=255)
                    caAAATCCATCGGCAGGTTTGTCGTTAGTGGCTCGTGCTGGAGGGATGATTGTTGGGAGCATTAAACTTGATGGCAGTGAAATTGCCATTGGTGAGGTGc                                                                        >  1:2549434/1‑100 (MQ=255)
                       aaTCCATCGGCAGGTTTGTCGTTAGTGGCTCGTGCTGGAGGGATGATTGTTGGGAGCATTAAACTTGATGGCAGTGAAATTGCCATTGGTGAGGTGCCGt                                                                     <  2:1743068/100‑1 (MQ=255)
                       aaTCCATCGGCAGGTTTGTCGTTAGTGGCTCGTGCTGGAGGGATGATTGTTGGGAGCATTAAACTTGATGGCAGTGAAATTGCCATTGGTGAGGTGCCGt                                                                     <  2:2871679/100‑1 (MQ=255)
                         tCCATCGGCAGGTTTGTCGTTAGTGGCTCGTGCTGGAGGGATGATTGTTGGGAGCATTAAACTTGATGGCAGTGAAATTGCCATTGGTGAGGTGCCGTTa                                                                   <  2:4124632/100‑1 (MQ=255)
                          ccATCGGCAGGTTTGTCGTTAGTGGCTCGTGCTGGAGGGATGAGTGTTGGGAGCAGTAAAATTGATGGCGGTGAAATTGCCATTGGTGGGGTGCCGtt                                                                    >  1:3148800/1‑98 (MQ=255)
                           cATCGGCAGGTTTGTCGTTAGTGGCTCGTGCTGGAGGGATGATTGTTGGGAGCATTAAACTTGGTGGCAGTGAAATTGCCATTGGTGAGGTGCCGTTAAc                                                                 >  1:476607/1‑100 (MQ=255)
                           cATCGGCAGGTTTGTCGTTAGTGGCTCGTGCTGGAGGGATGATTGTTGGGAGCATTAAACTTGATGGCAGTGAAATTGCCATTGGTGAGGTGCCGTTAAc                                                                 <  2:1303867/100‑1 (MQ=255)
                           cATCGGCAGGTTTGTCGTTAGTGGCTCGTGCTGGAGGGATGATTGTTGGGAGCATTAAACTTGATGGCAGTGAAATTGCCATTGGTGAGGTGCCGTTAAc                                                                 <  2:720096/100‑1 (MQ=255)
                           cATCGGCAGGTTTGTCGTTAGTGGCTCGTGCTGGAGGGATGATTGTTGGGAGCATTAAACTTGATGGCAGTGAAATTGCCATTGGTGAGGTGCCGTTAAc                                                                 >  1:4270100/1‑100 (MQ=255)
                           cATCGGCAGGTTTGTCGTTAGTGGCTCGTGCTGGAGGGATGAGTGTTGGGAGCATTAAACTTGATGGCAGTGGAATTGCCATTg                                                                                 >  1:4369297/1‑84 (MQ=255)
                            aTCGGCAGGTTTGTCGTTAGTGGCTCGTGCTGGAGGGATGATTGTTGGGAGCATTAAACTTGATGGCAGTGAAATTGCCATTGGTGAGGTGCCGTTAAcc                                                                <  2:1362577/100‑1 (MQ=255)
                            aTCGGCAGGTTTGTCGTTACTGGCCCGTGCTGGAGGGATGGTTGTTGGGAGCATTAAACTTGATGGCAGTGTATTTTCCATTGGTCAGGTGCCGTTAAcc                                                                >  1:1539231/1‑100 (MQ=255)
                             tCGGCAGGTTTGTCGTTAGTGGCTCGTGCTGGAGGGATGATTGTTGGGAGCATTAAACTTGATGGCAGTGAAATTGCCATTGGTGAGGTGCCGTTAACCt                                                               <  1:2271379/100‑1 (MQ=255)
                              cGGCAGGTTTGTCGTTAGTGGCTCGTGCTGGAGGGATGATTGTTGGGAGCATTAAACTTGATGGCAGTGAAATTGCCATTGGTGAGGTGCCGTTAACCtt                                                              <  1:1847032/100‑1 (MQ=255)
                                 cAGGTTTGTCGTTAGTGGCTCGTGCTGGAGGGATGATTGTTGGGAGCATTAAACTTGATGGCAGTGAAATTGCCATTGGTGAGGTGCCGTTAACCTTCAt                                                           <  2:923068/100‑1 (MQ=255)
                                    gTTTGTCGTTAGTGGCTCGTGCTGGAGGGATGATTGTTGGGAGCATTAAACTTGATGGCAGTGAAATTGCCATTGGTGAGGTGCCGTTAACCTTCATCGt                                                        <  1:2834638/100‑1 (MQ=255)
                                    gTTTGTCGTTAGTGGCTCGTGCTGGAGGGATGATTGTTGGGAGCATTAAACTTGATGGCAGTGAAATTGCCATTGGTGAGGTGCCGTTAACCTTCATCGt                                                        <  1:547082/100‑1 (MQ=255)
                                     tttGTCGTTAGTGGCTCGTGCTGGAGGGATGATTGTTGGGAGCATTAAACTTGATGGCAGTGAAATTGCCATTGGTGAGGTGCCGTTAACCTTCAtagat                                                       >  2:1713065/1‑96 (MQ=255)
                                     tttGTCGTTAGTGGCTCGTGCTGGAGGGATGATTGTTGGGAGCATTAAACTTGATGGCAGTGAAATTGCCATTGGTGAGGTGCAGTTAACCTTCATCGtt                                                       <  1:1941067/100‑1 (MQ=255)
                                     tttGTCGTTAGTGGCTAGAGCTGGAGGGATGATTGTTGGGAGCATTAAACTTGATGGCAGTGAAATTGCCATTGGTGAGGTGCCGTTAACCTTCATCGtt                                                       <  1:1738069/100‑1 (MQ=255)
                                      ttGTCGTTAGTGGCTCGTGCTGGAGGGATGATTGTTGGGAGCATTAAACTTGATGGCAGTGAAATTGCCATTGGTGAGGTGCCGTTAACCTTCATCGTTg                                                      <  2:3106856/100‑1 (MQ=255)
                                       tGTCGTTAGTGGCTCGTGCTGGAGGGATGATTGTTGGGAGCATTAAACTTGATGGCAGTGAAATTGCCATTGGTGAGGTGCCGTTAACCTTCATCGTTGa                                                     >  2:2922750/1‑100 (MQ=255)
                                        gTCGTTAGTGGCTCGTGCTGGAGGGATGATTGTTGGGAGCATTAAACTTGATGGCAGTGAAATTGCCATTGGTGAGGTGCCGTTAACCTTCATCGTTGa                                                     >  1:556699/1‑99 (MQ=255)
                                         tCGTTAGTGGCTCGTGCTGGAGGGATGATTGTTGGGAGCATTAAACTTGATGGCAGTGAAATTGCCATTGGTGAGGTGCCGTTAACCTTCATCGTTGATg                                                   <  2:1657770/100‑1 (MQ=255)
                                           gTTAGTGGCTCGTGCTGGAGGGATGATTGTTGGGAGCATTAAACTTGATGGCAGTGAAATTGCCATTGGTGAGGTGCCGTTAACCTTCATCGTTGATGCa                                                 >  1:2034001/1‑100 (MQ=255)
                                           gTTAGTGGCTCGTGCTGGAGGGATGATTGTTGGGAGCATTAAACTTGATGGCAGTGAAATTGCCATTGGTGAGGTGCCGTTAACCTTCATCGTTGATGCa                                                 <  2:3420290/100‑1 (MQ=255)
                                            ttAGTGGCTCGTGCTGGAGGGATGATTGTTGGGAGCATTAAACTTGATGGCAGTGAAATTGCCATTGGTGAGGTGCCGTTAACCTTCATCGTTGATGCAg                                                <  2:1162548/100‑1 (MQ=255)
                                             tAGTGGCTCGTGCTGGAGGGATGATTGTTGGGAGCATTAAACTTGATGGCAGTGAAATTGCCATTGGTGAGGTGCCGTTAACCTTCATCGTTGATGCAGa                                               <  2:1270303/100‑1 (MQ=255)
                                               gTGGCTCGTGCTGGAGGGATGATTGTTGGGAGCATTAAACTTGATGGCAGTGAAATTGCCATTGGTGAGGTGCCGTTAACCTTCATCGTTGATGCAGATc                                             >  1:3355343/1‑100 (MQ=255)
                                               gTGGCTCGTGCTGGAGGGATGATTGTTGGGAGCATTAAACTTGATGGCAGTGAAATTGCCATTGGTGAGGTGCCGTTAACCTTCATCGTTGATGCAGATc                                             <  1:2775239/100‑1 (MQ=255)
                                               gTGGCTCGTGCTGGAGGGATGATTGTTGGGAGCATTAAACTTGATGGCAGTGAAATTGCCATTGGTGAGGTGCCGTTAACCTTCATCGTTGATGCAGATc                                             <  1:2665778/100‑1 (MQ=255)
                                                 ggCTCGTGCTGGAGGGATGATTGTTGGGAGCATTAAACTTGATGGCAGTGAAATTGCCATTGGTGAGGTGCCGTTAACCTTCATCGTTGATGCAGATCaa                                           >  2:1383117/1‑100 (MQ=255)
                                                 ggCTCGTGCTGGAGGGATGATTGTTGGGAGCATTAAACTTGATGGCAGTGAAATTGCCATTGGTGAGGTGCCGTTAACCTTCATCGTTGATGCAGATCaa                                           <  2:4274348/100‑1 (MQ=255)
                                                 ggCTCGTGCTGGAGGGATGATTGTTGGGAGCATTAAACTTGATGGCAGTGAAATTGCCATTGGTGAGGTGCCGTTAACCTTCATAGTTGATGCAGATCaa                                           >  2:4335162/1‑100 (MQ=255)
                                                  gCTCGTGCTGGAGGGATGATTGTTGGGAGCATTAAACTTGATGGCAGTGAAATTGCCATTGGTGAGGTGCCGTTAACCTTCATCGTTGATGCAGATCAAt                                          <  2:2758266/100‑1 (MQ=255)
                                                   cTCGTGCTGGAGGGATGATTGTTGGGAGCATTAAACTTGATGGCAGTGAAATTGCCATTGGTGAGGTGCCGTTAACCTTCATCGTTGATGCAGATCAATg                                         <  1:2491643/100‑1 (MQ=255)
                                                   cTCGTGCTGGAGGGATGATTGTTGGGAGCATTAAACTTGATGGCAGTGAAATTGCCATTGGTGAGGTGCCGTTAACCTTCATCGTTGATGCAGATCAATg                                         <  2:694391/100‑1 (MQ=255)
                                                   cTCGTGCTGGAGGGATGATTGTTGGGAGCATTAAACTTGATGGCAGTGAAATTGCCATTGGTGAGGTGCCGTTAACCTTCATCGTTGATGCAGATCAATg                                         >  2:2742509/1‑100 (MQ=255)
                                                     cGTGCTGGAGGGATGATTGTTGGGAGCATTAAACTTGATGGCAGTGAAATTGCCATTGGTGAGGTGCCGTTAACCTTCATCGTTGATGCAGATCAATGGc                                       <  2:1566828/100‑1 (MQ=255)
                                                      gTGCTGGAGGGATGATTGTTGGGAGCATTAAACTTGATGGCAGTGAAATTGCCATTGGTGAGGTGCCGTTAACCTTCATCGTTGATGCAGATCAATGGCt                                      <  2:53959/100‑1 (MQ=255)
                                                      gTGCTGGAGGGATGATTGTTGGGAGCATTAAACTTGATGGCAGTGAAATTGCCATTGGTGAGGTGCCGTTAACCTTCATCGTTGATGCAGATCAATGGCt                                      >  1:4078784/1‑100 (MQ=255)
                                                      gTGCTGGAGGGATGATTGTTGGGAGCATTAAACTTGATGGCAGTGAAATTGCCATTGGTGAGGTGCCGTTAACCTTCATCGTTGATGCAGATCAATGGCt                                      <  2:608104/100‑1 (MQ=255)
                                                       tGCTGGAGGGATGATTGTTGGGAGCATTAAACTTGATGGCAGTGAAATTGCCATTGGTGAGGTGCCGTTAACCTTCATCGTTGATGCAGATCAATGGCTg                                     <  2:2024013/100‑1 (MQ=255)
                                                        gCTGGAGGGATGCTTGTTGGGAGCATTAAACTTGATGGCAGTGGAATTGCGAGTGGTGAGGTGCCGTTAACCTTCATCGTTGATGAAGATCAATGGCTGt                                    >  1:1296574/1‑100 (MQ=255)
                                                         ntGGAGGGATGATTGTTGGGAGCATTAAACTTGATGGCAGTGAAATTGCGATTGGTGAGGTGCCATTAGCCTTCATCGTTGATGCAGATCAATGGc                                       >  1:2051651/2‑96 (MQ=255)
                                                         cTGGAGGGATGATTGTTGGGAGCATTAAACTTGATGGCAGTGAAATTGCCATTGGTGAGGTGCCGTTAACCTTCATCGTTGATGCAGATCAATGGCTGtt                                   >  1:461239/1‑100 (MQ=255)
                                                          tccgatcttgatTGTTGGGAGCATTAAACTTGATGGCAGTGAAATTGCCATTGGTGAGGTGCCGTTAACCTTCATCGTTGATGCAGATCAATGGCTGTTa                                  <  1:1299519/92‑1 (MQ=255)
                                                            gAGGGATGATTGTTGGGAGCATTAAACTTGATGGCAGTGAAATTGCCATTGGTGAGGTGCCGTTAACCTTCATCGTTGATGCAGATCAATGGCTGTTACa                                <  2:304743/100‑1 (MQ=255)
                                                              gggATGATTGTTGGGAGCATTAAACTTGATGGCAGTGAAATTGCCATTGGTGAGGTGCCGTTAACCTTCATCGTTGATGCAGATCAATGGCTGTTACAgg                              <  2:2164276/100‑1 (MQ=255)
                                                                gatgatTGTTGGGAGCATTAAACTTGATGGCAGTGAAATTGCCATTGGTGAGGTGCCGTTAACCTTCATCGTTGATGCAGATCAATGGCTGTTACAGGGa                            >  1:2023927/1‑100 (MQ=255)
                                                                  tgatTGTTGGGAGCATTAAACTTGATGGCAGTGAAATTGCCATTGGTGAGGTGCCGTTAACCTTCATCGTTGATGCAGATCAATGGCTGTTACAGGGACa                          >  2:2030229/1‑100 (MQ=255)
                                                                   gatTGTTGGGAGCATTAAACTTGATGGCAGTGAAATTGCCATTGGTGAGGTGCCGTTAACCTTCATCGTTGATGCAGATCAATGGCTGTTACAGGGACAg                         >  1:2957221/1‑100 (MQ=255)
                                                                   gatTGTTGGGAGCATTAAACTTGATGGCAGTGAAATTGCCATTGGTGAGGTGCCGTTAACCTTCATCGTTGATGCAGATCAATGGCTGTTACAGGGACAg                         >  2:1316310/1‑100 (MQ=255)
                                                                   gatTGTTGGGAGCATTAAACTTGATGGCAGTGAAATTGCCATTGGTGAGGTGCCGTTAACCTGCATCGTTGATGCAGATCAATGGCTGTTACAGGGACa                          <  1:3128788/99‑1 (MQ=255)
                                                                    atTGTTGGGAGCATTAAACTTGATGGCAGTGAAATTGCCATTGGTGAGGTGCCGTTAACCTTCATCGTTGATGCAGATCAATGGCTGTTACAGGGACAgg                        >  2:2935683/1‑100 (MQ=255)
                                                                     ttgttgGGAGCATTAAACTTGATGGCAGTGAAATTGCCATTGGTGAGGTGCCGTTAACCTTCATCGTTGATGCAGATCAATGGCTGTTACAGGGACAGGc                       <  1:4161804/100‑1 (MQ=255)
                                                                      tgttgGGAGCATTAAACTTGATGGCAGTGAAATTGCCATTGGTGAGGTGCCGTTAACCTGCATCGTTGATGAAGATCAATGGCTGTTACAGGGACAGGc                       >  1:1075757/1‑99 (MQ=255)
                                                                        ttgGGAGCATTAAACTTGATGGCAGTGAAATTGCCATTGGTGAGGTGCCGTTAACCTTCATCGTTGATGCAGATCAATGGCTGTTACAGGGACAGGcttc                    <  1:1174382/100‑1 (MQ=255)
                                                                        ttgGGAGCATTAAACTAGATGGCAGTGAAATTGCCATTGGTGAGGTGCCGTTAACCTTCATCGTTGATGCAGATCAATGGCTGTTACAGGGACAGGcttc                    <  1:2583725/100‑1 (MQ=255)
                                                                          gggAGCATTAAACTTGATGGCAGTGAAATTGCCATTGGTGAGGTGCCGTTAACCTTCATCGTTGATGCAGATCAATGGCTGTTACAGGGACAGGcttct                   >  1:3536574/1‑99 (MQ=255)
                                                                           ggAGCATTAAACTTGATGGCAGTGAAATTGCCATTGGTGAGGTGCCGTTAACCTTCATCGTTGATGCAGATCAATGGCTGTTACAGGGACAGGCTTCTTg                 >  2:826323/1‑100 (MQ=255)
                                                                           ggAGCATTAAACTTGATGGCAGTGAAATTGCCATTGGTGAGGTGCCGTTAACCTTCATCGTTGATGCAGATCAATGGCTGTTACAGGGACAGGCTTCTTg                 <  1:1000194/100‑1 (MQ=255)
                                                                           ggAGCATTAAACTTGATGGCAGTGAAATTGCCATTGGTGAGGTGCCGTTAACCTTCATCGTTGATGCAGATCAATGGCTGTTACAGGGACAGGCTTCTTg                 <  2:1826734/100‑1 (MQ=255)
                                                                            gAGCATTAAACTTGATGGCAGTGAAATTGCCATTGGTGAGGTGCCGTTAACCTTCATCGTTGATGCAGATCAATGGCTGTTACAGGGACAGGCTTCTTGc                <  2:1964874/100‑1 (MQ=255)
                                                                             aGCATTAAACTTGATGGCAGTGAAATTGCCATTGGTGAGGTGCCGTTAACCTTCATCGTTGATGCAGATCAATGGCTGTTACAGGGACAGGCTTCTTGCa               <  1:2865202/100‑1 (MQ=255)
                                                                              gCATTAAACTTGATGGCAGTGAAATTGCCATTGGTGAGGTGCCGTTAACCTTCATCGTTGATGCAGATCAATGGCTGTTACAGGGACAGGCTTCTTGCan              <  2:4042585/100‑2 (MQ=255)
                                                                              gCATTAAACTTGATGGCAGTGAAATTGCCATTGGTGAGGTGCCGTTAACCTTCATCGTTGATGCAGATCAATGGCTGTTACAGGGACAGGCTTCTTGCAg              >  2:1543800/1‑100 (MQ=255)
                                                                               cATTAAACTTGATGGCAGTGAAATTGCCATTGGTGAGGTGCCGTTAACCTTCATCGTTGATGCAGATCAATGGCTGTTACAGGGACAGGCTTCTTGCAgt             >  2:3829304/1‑100 (MQ=255)
                                                                                aTTAAACTTGATGGCAGTGAAATTGCCATTGGTGAGGTGCCGTTAACCTTCATCGTTGATGCAGATCAATGGCTGTTACAGGGACAGGCTTCTTGCAgtg            <  2:2642896/100‑1 (MQ=255)
                                                                                 ttAAACTTGATGGCAGTGAAATTGCCATTGGTGAGGTGCCGTTAACCTTCATCGTTGATGCAGATCAATGGCTGTTACAGGGACAGGCTTCTTGCAgtgt           >  2:224816/1‑100 (MQ=255)
                                                                                   aaaCTTGATGGCAGTGAAATTGCCATTGGTGAGGTGCCGTTAACCTTCATCGTTGATGCAGATCAATGGCTGTTACAGGGACAGGCTTCTTGCAGTGTGc         <  2:2391126/100‑1 (MQ=255)
                                                                                    aaCTTGATGGCAGTGAAATTGCCATTGGTGAGGTGCCGTTAACCTTCATCGTTGATGCAGATCAGTGGCTGTTACGGGGACAGGCTTCTTGCAGTGTGCg        >  1:750756/1‑100 (MQ=255)
                                                                                     aCTTGATGGCAGTGAAATTGCCATTGGTGAGGTGCCGTTAACCTTCATCGTTGATGCAGATCAATGGCTGTTACAGGGACAGGCTTCTTGCAGTGTGCGa       <  2:2832709/100‑1 (MQ=255)
                                                                                      cTTGATGGCAGTGAAATTGCCATTGGTGAGGTGCCGTTAACCTTCATCGTTGATGCAGATCAATGGCTGTTACAGGGACAGGCTTCTTGCAGTGTGCGaa      >  2:328487/1‑100 (MQ=255)
                                                                                      cTTGATGGCAGTGAAATTGCCATTGGTGAGGTGCCGTTAACCTTCATCGTTGATGCAGATCAATGGCTGTTACAGGGACAGGCTTCTTGCAGTGTGCGaa      <  1:1886592/100‑1 (MQ=255)
                                                                                       ttGATGGCAGTGAAATTGCCATTGGTGAGGTGCCGTTAACCTTCATCGTTGATGCAGATCAATGGCTGTTACAGGGACAGGCTTCTTGCAGTGTGCGAag     <  2:932750/100‑1 (MQ=255)
                                                                                        tgatggCAGTGAAATTGCCATTGGTGAGGTGCCGTTAACCTTCATCGTTGATGCAGATCAATGGCTGTTACAGGGACAGGCTTCTTGCAGTGTGCGAagc    <  2:3697251/100‑1 (MQ=255)
                                                                                             |                                                                                                
GCGAAGTGAGATTTGGCAGGCAAAATCCATCGGCAGGTTTGTCGTTAGTGGCTCGTGCTGGAGGGATGATTGTTGGGAGCATTAAACTTGATGACAGTGAAATTGCCATTGGTGAGGTGCCGTTAACCTTCATCGTTGATGCAGATCAATGGCTGTTACAGGGACAGGCTTCTTGCAGTGTGCGAAGCAG  >  NC_000913/4573435‑4573624

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A