Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A9 F255 I1 R1 77 120.7 6116409 93.2% 5700493 99.9

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 4,295,744 G→A A422T (GCT→ACT)  gltP → glutamate/aspartate:proton symporter

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,295,7440GA98.4% 407.2 / ‑3.4 124A422T (GCT→ACT) gltPglutamate/aspartate:proton symporter
Reads supporting (aligned to +/- strand):  ref base G (1/1);  new base A (65/57);  total (66/58)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.91e-01

Only 100 of 124 total aligned reads displayed.

CGACATGGCGCGTACTGCGCTGAACGTGGTGGGTAATGCGCTGGCGGTGCTGGTGATTGCCAAGTGGGAACACAAATTTGACCGTAAGAAAGCGCTGGCTTATGAGCGTGAAGTGCTGGGCAAATTTGATAAAACTGCGGATCAATAATTGAAGATTGCCGGGGATATCCACCCGGCAATGTGTGAATGCCTG  >  NC_000913/4295647‑4295839
                                                                                                 |                                                                                               
cGACATGGCGCGTACTGCGCTGAACGTGGTGGGTAATGCGCTGGCGGTGCTGGTGATTGCCAAGTGGGAACACAAATTTGACCGTAAGAAAGCGCTGACt                                                                                               <  2:2811313/100‑1 (MQ=255)
 gACATGGCGCGTACTGCGCTGAACGTGGTGGGTACTGCGCTGGCGGTGCTGGTGATTGCCAAGTGGGAACACAAATTTGACCGTAAGACAGCGCTGACtt                                                                                              <  2:1612618/100‑1 (MQ=255)
   cATGGCGCGTACTGCGCTGAACGTGGTGGGTAATGCGCTGGCGGTGCTGGTGATTGCCAAGTGGGAACACAAATTTGACCGTAAGAAAGCGCTGACTTAt                                                                                            <  2:62181/100‑1 (MQ=255)
    aTGGCGCGTACTGCGCTGAACGTGGTGGGTAATGCGCTGGCGGTGCTGGTGATTGCCAAGTGGGAACACAAATTTGACCGTAAGAAAGCGCTGACTTAtn                                                                                           <  2:1442777/100‑2 (MQ=255)
    aTGGCGCGTACTGCGCTGAACGTGGTGGGTAATGCGCTGGCGGTGCTGGTGATTGCCAAGTGGGAACACAAATTTGACCGTAAGAAAGCGCTGACTTATg                                                                                           <  1:594728/100‑1 (MQ=255)
      ggCGCGTACTGCGCTGAACGTGGTGGGTAATGCGCTGGCGGTGCTGGTGATTGCCAAGTGGGAACACAAATTTGACCGTAAGAAAGCGCTGACTTATGAg                                                                                         <  1:2631122/100‑1 (MQ=255)
      ggCGCGTACTGCGCTGAACGTGGTGGGTAATGCGCTGGCGGTGCTGGTGATTGCCAAGTGGGAACACAAATTTGACCGTAAGAAAGCGCTGACTTATGAg                                                                                         >  2:146936/1‑100 (MQ=255)
      ggCGCGTACTGCGCTGAACGTGGTGGGTAATGCGCTGGCGGTGCTGGTGATTGCCAAGTGGGAACACAAATTTGACCGTAAGAAAGCGCTGACTTATGAg                                                                                         >  1:1336921/1‑100 (MQ=255)
        cgcgTACTGCGCTGAACGTGGTGGGTAATGCGCTGGCGGTGCTGGTGATTACCAAGTGGGAACACAAATTTGACCGTAAGAAAGCGCTGACTTATGAGCg                                                                                       >  1:728193/1‑100 (MQ=255)
         gcgTACTGCGCTGAACGTGGTGGGTAATGCGCTGGCGGTGCTGGTGATTGCCAAGTGGGGACACAAATTTGACCGTAAGAAAGCGCTGACTTa                                                                                             <  2:1828824/93‑1 (MQ=255)
            naCTGCGCTGAACGTGGTGGGTAATGCGCTGGCGGTGCTGGTGATTGCCAAGTGGGAACACAAATTTGACCGTAAGAAAGCGCTGACTTATGAGCGTGaa                                                                                   >  1:1490459/2‑100 (MQ=255)
             aCTGCGCTGAACGTGGTGGGTAATGCGCTGGCGGTGCTGGTGATTGCCAAGTGGGAACACAAATTTGACCGTAAGAAAGCGCTGACTTATGAGCGTGAAg                                                                                  >  1:16905/1‑100 (MQ=255)
              cTGCGCTGAACGTGGTGGGTAATGCGCTGGCGGTGCTGGTGATTGCCAAGTGGGAACACAAATTTGACCGTAAGAAAGCGCTGACTTATGAGCGTGAAGt                                                                                 >  2:207797/1‑100 (MQ=255)
              cTGCGCTGAACGTGGTGGGTAATGCGCTGGCGGTGCTGGTGATTGCCAAGTGGGAACACAAATTTGACCGTAAGAAAGCGCTGACTTATGAGCGTGAAGt                                                                                 >  1:1928040/1‑100 (MQ=255)
                gcgcTGAACGTGGTGGGTAATGCGCTGGCGGTGCTGGTGATTGCCAAGTGGGAACACAAATTTGACCGTAAGAAAGCGCTGACTTATGAGCGTGAAGTGc                                                                               >  2:2468019/1‑100 (MQ=255)
                gcgcTGAACGTGGTGGGTAATGCGCTGGCGGTGCTGGTGATTGCCAAGTGGGAACACAAATTTGACCGTAAGAAAGCGCTGACTAATGAGCGTGAAGTGc                                                                               >  1:3011759/1‑100 (MQ=255)
                  gcTGAACGTGGTGGGTAATGCGCTGGCGGTGCTGGTGATTGCCAAGTGGGAACACAAATTTGACCGTAAGAAAGCGCTGACTTATGAGCGTGAAGTGCta                                                                             <  2:305366/100‑2 (MQ=255)
                  gcTGAACGTGGTGGGTAATGCGCTGGCGGTGCTGGTGATTGCCAAGTGGGAACACAAATTTGACCGTAAGAAAGCGCTGACTTATGAGCGTGAAGTGCTg                                                                             <  1:1344967/100‑1 (MQ=255)
                  gcTGAACGTGGTGGGTAATGCGCTGGCGGTGCTGGTGATTGCCAAGTGGGAACACAAATTTGACCGTAAGAAAGCGCTGACTTATGAGCGTGAAGTGCTg                                                                             >  1:1767006/1‑100 (MQ=255)
                  gcTGAACGTGGTGGGTAATGCGCTGGCGGTGCTGGTGATTGCCAAGTGGGAACACAAATTTGACCGTAAGAAAGCGCTGACTTATGAGCGTGAAGTGCTg                                                                             >  2:1494155/1‑100 (MQ=255)
                  gcTGAACGTGGTGGGTAATGCGCTGGCGGTGCTGGTGATTGCCAAGTGGGAACACAAATTTGACCGTAAGAAAGCGCTGACTTATGAGCGTGAAGTGCTg                                                                             >  1:1116356/1‑100 (MQ=255)
                   cTGAACGTGGTGGGTAATGCGCTGGCGGTGCTGGTGATTGCCAAGTGGGAACACAAATTTGACCGTAAGAAAGCGCTGGCTTATGAGCGTGAAGTGCTgg                                                                            <  2:2864157/100‑1 (MQ=255)
                   cTGAACGTGGTGGGTAATGCGCTGGCGGTGCTGGTGATTGCCAAGTGGGAACACAAATTTGACCGTAAGAAAGCGCTGACTTATGAGCGTGAAGTGCTgg                                                                            >  2:53346/1‑100 (MQ=255)
                   cTGAACGTGGTGGGTAATGCGCTGGCGGTGCTGGTGATTGCCAAGTGGGAACACAAATTTGACCGTAAGAAAGCGCTGACTTATGAGCGTGAAGTGCTgg                                                                            >  1:1532630/1‑100 (MQ=255)
                     gAACGTGGTGGGTAATGCGCTGGCGGTGCTGGTGATTGCCAAGTGGGAACACAAATTTGACCGTAAGAAAGCGCTGACTTATGAGCGTGAAGTGCTGGGc                                                                          <  2:136706/100‑1 (MQ=255)
                           ggtggGTAATGCGCTGGCGGTGCTGGTGATTGCCAAGTGGGAACACAAATTTGACCGTAAGAAAGCGCTGACTTATGAGCGTGAAGTGCTGGGCAAAttt                                                                    >  1:2458053/1‑100 (MQ=255)
                           ggtggGTAATGCGCTGGCGGTGCTGGTGATTGCCAAGTGGGAACACAAATTTGACCGTAAGAAAGCGCTGACTTATGAGCGTGAAGTGCTGGGCAAAttt                                                                    <  2:1626104/100‑1 (MQ=255)
                           ggtggGTAATGCGATGGCGGTGCTGGTGATTGCCAAGTTGGAACACAAATTTGACCGTAAGAAAGCGCTGACTTATGAGCGTGAAGTGCTGGGCAAAttt                                                                    >  2:1881889/1‑100 (MQ=255)
                            gtggGTAATGCGCTGGCGGTGCTGGTGATTGCCAAGTGGGAACACAAATTTGACCGTAAGAAAGCGCTGACTTATGAGCGTGAAGTGCTGGGCAAATTTg                                                                   <  1:1069120/100‑1 (MQ=255)
                             tggGTAATGCGCTGGCGGTGCTGGTGATTGCCAAGTGGGAACACAAATTTGACCGTAAGAAAGCGCTGACTTATGAGCGTGAAGTGCTGGGCAAATTTGa                                                                  >  1:484790/1‑100 (MQ=255)
                             tggGTAATGCGCTGGCGGTGCTGGTGATTGCCAAGTGGGAACACAAATTTGACCGTAAGAAAGCGCTGACTTATGAGCGTGAAGTGCTGGGCAAATTTGa                                                                  >  1:826469/1‑100 (MQ=255)
                              gggTAATGCGCTGGCGGTGCTGGTGATTGCCAAGTGGGAACACAAATTTGACCGTAAGAAAGCGCTGACTTATGAGCGTGAAGTGCTGGGCAAATTTGAt                                                                 >  1:1036963/1‑100 (MQ=255)
                              gggTAATGCGCTGGCGGTGCTGGTGATTGCCAAGTGGGAACACAAATTTGACCGTAAGAAAGCGCTGACTTATGAGCGTGAAGTGCTGGGCAAATTTGAt                                                                 <  1:1792785/100‑1 (MQ=255)
                               ggTAATGCGCTGGCGGTGCTGGTGATTGCCAAGTGGGAACACAAATTTGACCGTAAGAAAGCGCTGACTTATGAGCGTGAAGTGCTGGGCAAATTTGATa                                                                <  1:2794911/100‑1 (MQ=255)
                                gTAATGCGCTGGCGGTGCTGGTGATTGCCAAGTGGGAACACAAATTTGACCGTAAGAAAGCGCTGACTTATGAGCGTGAAGTGCTGGGCAAATTTGATaa                                                               >  1:2291739/1‑100 (MQ=255)
                                 tAATGCGCTGGCGGTGCTGGTGATTGCCAAGTGGGAACACAAATTTGACCGTAAGAAAGCGCTGACTTATGAGCGTGAAGTGCTGGGCAAATTTGATaaa                                                              <  2:314175/100‑1 (MQ=255)
                                   aTGCGCTGGCGGTGCTGGTGATTGCCAAGTGGGAACACAAATTTGACCGTAAGAAAGCGCTGACTTATGAGCGTGAAGTGCTGGGCAAATTTGATAAAAc                                                            <  2:2076309/100‑1 (MQ=255)
                                   aTGCGCTGGCGGTGCTGGTGATTGCCAAGTGGGAACACAAATTTGACCGTAAGAAAGCGCTGACTTATGAGCGTGAAGTGCTGGGCAAATTTGATAAAAc                                                            <  1:2154826/100‑1 (MQ=255)
                                   aTGCGCTGGCGGTGCTGGTGATTGCCAAGTGGGAACACAAATTTGACCGTAAGAAAGCGCTGACTTATGAGCGTGAAGTGCTGGGCAAATTTGATAAAAc                                                            <  2:274037/100‑1 (MQ=255)
                                   aTGCGCTGGCGGTGCTGGTGATTGCCAAGTGGGAACACAAATTTGACCGTAAGAAAGCGCTGACTTATGAGCGTGAAGTGCTGGGCAAATTTGATAAAAc                                                            >  2:1079522/1‑100 (MQ=255)
                                    tGCGCTGGCGGTGCTGGTGATTGCCAAGTGGGAACACAAATTTGACCGTAAGAAAGCGCTGACTTATGAGCGTGAAGTGCTGGGCAAATTTGATAAAACt                                                           >  2:1629794/1‑100 (MQ=255)
                                    tGCGCTGGCGGTGCTGGTGATTGCCAAGTGGGAACACAAATTTGACCGTAAGAAAGCGCTGACTTATGAGCGTGAAGTGCTGGGCAAATTTGATAAAACt                                                           <  1:1430816/100‑1 (MQ=255)
                                      cgcTGGCGGTGCTGGTGATTGCCAAGTGGGAACACAAATTTGACCGTAAGAAAGCGCTGACTTATGAGCGTGAAGTGCTGGGCAAATTTGATAAAACTGc                                                         <  2:2678958/100‑1 (MQ=255)
                                       gcTGGCGGTGCTGGTGATTGCCAAGTGGGAACACAAATTTGACCGTAAGAAAGCGCTGACTTATGAGCGTGAAGTGCTGGGCAAATTTGATAAAACTGCg                                                        <  2:2206740/100‑1 (MQ=255)
                                       gcTGGCGGTGCTGGTGATTGCCAAGTGGGAACACAAATTTGACCGTAAGAAAGCGCTGACTTATGAGCGTGAAGTGCTGGGCAAATTTGATAAAACTGCg                                                        <  2:1291581/100‑1 (MQ=255)
                                       gcTGGCGGTGCTGGTGATTGCCAAGTGGGAACACAAATTTGACCGTAAGAAAGCGCTGACTTATGAGCGTGAAGTGCTGGGCAAATTTGATAAAACTGCg                                                        >  1:1896908/1‑100 (MQ=255)
                                         tGGCGGTGCTGGTGATTGCCAAGTGGGAACACAAATTTGACCGTAAGAAAGCGCTGACTTATGAGCGTGAAGTGCTGGGCAAATTTGATAAAACTGCGGa                                                      <  1:1334733/100‑1 (MQ=255)
                                          ggCGGTGCTGGTGATTGCCAAGTGGGAACACAAATTTGACCGTAAGAAAGCGCTGACTTATGAGCGTGAAGTGCTGGGCAAATTTGATAAAACTGCGGAt                                                     >  1:2433435/1‑100 (MQ=255)
                                           gCGGTGCTGGTGATTGCCAAGTGGGAACACAAATTTGACCGTAAGAAAGCGCTGACTTATGAGCGTGAAGTGCTGGGCAAATTTGATAAAACTGCGGATc                                                    <  1:2284785/100‑1 (MQ=255)
                                            cGGTGCTGGTGATTGCCAAGTGGGAACACAAATTTGACCGTAAGAAAGCGCTGACTTATGAGCGTGAAGTGCTGGGCAAATTTGATAAAACTGCGGATCa                                                   <  2:654562/100‑1 (MQ=255)
                                            cGGTGCTGGTGATTGCCAAGTGGGAACACAAATTTGACCGTAAGAAAGCGCTGACTTATGAGCGTGAAGTGCTGGGCAAATTTGATAAAACTGCGGATCa                                                   <  2:494045/100‑1 (MQ=255)
                                               tGCTGGTGATTCCCAAGTGGGAACACAAATTTGACCGTAAGAAAGCGCTGACTTATGAGCGTGAAGTGCTGGGCAAATTTGATAAAACTGCGGATCaat                                                 <  2:2769579/99‑1 (MQ=255)
                                                gCTGGTGATTGCCAAGTGGGAACACAAATTTGACCGTAAGAAAGCGCTGACTTATGAGCGTGAAGTGCTGGGCAAATTTGATAAAACTGCGGATCaataa                                               >  2:2552500/1‑100 (MQ=255)
                                                 cTGGTGATTGCCAAGTGGGAACACAAATTTGACCGTAAGAAAGCGCTGACTTATGAGCGTGAAGTGCTGGGCAAATTTGATAAAACTGCGGATCaataat                                              <  1:623651/100‑1 (MQ=255)
                                                 cTGGTGATTGCCAAGTGGGAACACAAATTTGACCGTAAGAAAGCGCTGACTTATGAGCGTGAAGTGCTGGGCAAATTTGATAAAACTGCGGATCaataat                                              >  1:1063487/1‑100 (MQ=255)
                                                  tGGTGATTGCCAAGTGGGAACACAAATTTGACCGTAAGAAAGCGCTGACTTATGAGCGTGAAGTGCTGGGCAAATTTGATAAAACTGCGGATCAATAAtt                                             >  2:417419/1‑100 (MQ=255)
                                                   ggTGATTGCCAAGTGGGAACACAAATTTGACCGTAAGAAAGCGCTGACTTATGAGCGTGAAGTGCTGGGCAAATTTGATAAAACTGCGGATCAATAATTg                                            >  2:2232785/1‑100 (MQ=255)
                                                   ggTGATTGCCAAGTGGGAACACAAATTTGACCGTAAGAAAGCGCTGACTTATGAGCGTGAAGTGCTGGGCAAATTTGATAAAACTGCGGATCAATAATTg                                            >  2:2791037/1‑100 (MQ=255)
                                                   ggTGATTGCCAAGTGGGAACACAAATTTGACCGTAAGAAAGCGCTGACTTATGAGCGTGAAGTGCTGGGCAAATTTGATAAAACTGCGGATCAATAATTg                                            <  1:2724778/100‑1 (MQ=255)
                                                   ggTGATTGCCAAGTGGGAACACAAATTTGACCGTAAGAAAGCGCTGACTTATGAGCGTGAAGTGCTGGGCAAATTTGATAAAACTGCGGATCAATAATTg                                            <  2:1116344/100‑1 (MQ=255)
                                                    gtgagagCCAAGGGGGAGCAAAAGTTTGACCGTAAGAAAGCTCTGACTTATGAGCGTGAAGTGCTGGTCAAATTTGATAAAACTGCGGATCAATAATTg                                            <  2:2968678/93‑1 (MQ=255)
                                                    gTGATTGCCAAGTGGGAACACAAATTTGACCGTAAGAAAGCGCTGACTTATGAGCGTGAAGTGCTGGGCAAATTTGATAAAACTGCGGATCAATAATTGa                                           >  1:2472272/1‑100 (MQ=255)
                                                     tGATTGCCAAGTGGGAACACAAATTTGACCGTAAGAAAGCGCTGACTTATGAGCGTGAAGTGCTGGGCAAATTTGATAAAACTGCGGATCAATAATTGaa                                          <  1:1946472/100‑1 (MQ=255)
                                                      gATTGCCAAGTGGGAACACAAATTTGACCGTAAGAAAGCGCTGACTTATGAGCGTGAAGTGCTGGGCAAATTTGATAAAACTGCGGATCAATAATTGAAg                                         <  2:185874/100‑1 (MQ=255)
                                                         tGCCAAGTGGGAACACAAATTTGACCGTAAGAAAGCGCTGACTTATGAGCGTGAAGTGCTGGGCAAATTTGATAAAACTGCGGATCAATAATTGAAGAtt                                      >  1:887100/1‑100 (MQ=255)
                                                         tGCCAAGTGGGAACACAAATTTGACCGTAAGAAAGCGCTGACTTATGAGCGTGAAGTGCTGGGCAAATTTGATAAAACTGCGGATCAATAATTGAAGAtt                                      >  2:637608/1‑100 (MQ=255)
                                                          gCCAAGTGGGAACACAAATTTGACCGTAAGAAAGCGCTGACTTGTGAGCGTGAAGTGCTGGGCAAATTTGATAAAACTGCGGATCAATAATTGAAGATTg                                     <  2:1764323/100‑1 (MQ=255)
                                                          gCCAAGTGGGAACACAAATTTGACCGTAAGAAAGCGCTGACTTATGAGCGTGAAGTGCTGGGCAAATTTGATAAAACTGCGGATCAATAATTGAAGATTg                                     >  1:1717776/1‑100 (MQ=255)
                                                          gCCAAGTGGGAACACAAATTTGACCGTAAGAAAGCGCTGACTTATGAGCGTGAAGTGCTGGGCAAATTTGATAAAACTGCGGATCAATAATTGAAGATTg                                     >  1:3002203/1‑100 (MQ=255)
                                                          gCCAAGTGGGAACACAAATTTGACCGTAAGAAAGCGCTGACTTATGAGCGTGAAGTGCTGGGCAAATTTGATAAAACTGCGGATCAATAATTGAAGATTg                                     >  1:127276/1‑100 (MQ=255)
                                                          gCCAAGTGGGAACACAAATTTGACCGTAAGAAAGCGCTGACTTATGAGCGTGAAGTGCTGGGCAAATTTGATAAAACTGCGGATCAATAATTGAAGATTg                                     <  2:2770540/100‑1 (MQ=255)
                                                            cAAGTGGGAACACAAATTTGACCGTAAGAAAGCGCTGGCTTATGAGCGTGAAGTGCTGGGCAAATTTGATAAAACTGCGGATCAATAATTGAAGATTGcc                                   >  2:1462862/1‑100 (MQ=255)
                                                            cAAGTGGGAACACAAATTTGACCGTAAGAAAGCGCTGACTTATGAGCGTGAAGTGCTGGGCAAATTTGATAAAACTGCGGATCAATAATTGAAGATTGcc                                   >  2:1902724/1‑100 (MQ=255)
                                                            cAAGTGGGAACACAAATTTGACCGTAAGAAAGCGCTGACTTATGAGCGTGAAGTGCTGGGCAAATTTGATAAAACTGCGGATCAATAATTGAAGATTGcc                                   >  2:2187908/1‑100 (MQ=255)
                                                            cAAGTGGGAACACAAATTTGACCGTAAGAAAGCGCTGACTTATGAGCGTGAAGTGCTGGGCAAATTTGATAAAACTGCGGATCAATAATTGAAGATTGcc                                   >  2:537742/1‑100 (MQ=255)
                                                                 gggAACACAAATTTGACCGTAAGAAAGCGCTGACTTATGAGCGTGAAGTGCTGGGCAAATTTGATAAAACTGCGGATCAATAATTGAAGATTGCCGGGGa                              >  2:2797457/1‑100 (MQ=255)
                                                                  ggAACACAAATTTGACCGTAAGAAAGCGCTGACTTATGAGCGTGAAGTGCTGGGCAAATTTGATAAAACTGCGGATCAATAATTGAAGATTGCCGGGGat                             >  2:1545023/1‑100 (MQ=255)
                                                                      cacaAATTTGACCGTAAGAAAGCGCTGACTTATGAGCGTGAAGTGCTGGGCAAATTTGATAAAACTGCGGATCAATAATTGAAGATTGCCGGGGATATcc                         <  2:1612528/100‑1 (MQ=255)
                                                                      cacaAATTTGACCGTAAGAAAGCGCTGACTTATGAGCGTGAAGTGCTGGGCAAATTTGATAAAACTGCGGATCAATAATTGAAGATTGCCGGGGATATcc                         >  2:2720865/1‑100 (MQ=255)
                                                                       acaAATTTGACCGTAAGAAAGCGCTGACTTATGAGCGTGAAGTGCTGGGCAAATTTGATAAAACTGCGGATCAATAATTGAAGATTGCCGGGGATATCCa                        >  2:1036863/1‑100 (MQ=255)
                                                                        caAATTTGACCGTAAGAAAGCGCTGACTTATGAGCGTGAAGTGCTGGGCAAATTTGATAAAACTGCGGATCAATAATTGAAGATTGCCGGGGATATCCAc                       >  2:909421/1‑100 (MQ=255)
                                                                        caAATTTGACCGTAAGAAAGCGCTGACTTATGAGCGTGAAGTGCTGGGCAAATTTGATAAAACTGCGGATCAATAATTGAAGATTGCCGGGGATATCCAc                       >  1:633433/1‑100 (MQ=255)
                                                                         aaaTTTGACCGTAAGAAAGCGCTGACTTATGAGCGTGAAGTGCTGGGCAAATTTGATAAAACTGCGGATCAATAATTGAAGATTGCCGGGGATATCCAcc                      >  1:1412514/1‑100 (MQ=255)
                                                                           aTTTGACCGTAAGAAAGCGCTGACTTATGAGCGTGAAGTGCTGGGCAAATTTGATAAAACTGCGGATCAATAATTGAAGATTGCCGGGGATATCCACCCg                    >  1:196421/1‑100 (MQ=255)
                                                                             ttGACCGTAAGAAAGCGCTGACTTATGAGCGTGAAGTGCTGGGCAAATTTGATAAAACTGCGGATCAATAATTGAAGATTGCCGGGGATATCCACCCGGc                  <  2:3002162/100‑1 (MQ=255)
                                                                             ttGACCGTAAGAAAGCGCTGACTTATGAGCGTGAAGTGCTGGGCAAATTTGATAAAACTGCGGATCAATAATTGAAGATTGCCGGGGATATCCACCCGGc                  <  1:1023715/100‑1 (MQ=255)
                                                                                 ccGTAAGAAAGCGCTGACTTATGAGCGTGAAGTGCTGGGCAAATTTGATAAAACTGCGGATCAATAATTGAAGATTGCCGGGGATATCCACCCGGCAAtg              <  1:2791076/100‑1 (MQ=255)
                                                                                 ccGTAAGAAAGCGCTGACTTATGAGCGTGAAGTGCTGGGCAAATTTGATAAAACTGCGGATCAATAATTGAAGATTGCCGGGGATATCCACCCGGCAAtg              >  2:2531569/1‑100 (MQ=255)
                                                                                 ccGTAAGAAAGCGCTGACTTATGAGCGTGAAGTGCTGGGCAAATTTGATAAAACTGCGGATCAATAATTGAAGATTGCCGGGGATATCCACCCGGCAAtg              >  2:79592/1‑100 (MQ=255)
                                                                                 ccGTAAGAAAGCGCTGACTTATGAGCGTGAAGTGCTGGGCAAATTTGATAAAACTGCGGATCAATAATTGAAGATTGCCGGGGATATCCACCCGGCAAtg              <  2:2768270/100‑1 (MQ=255)
                                                                                  cGTAAGAAAGCGCTGACTTATGAGCGTGAAGTGCTGGGCAAATTTGATAAAACTGCGGATCAATAATTGGAGATTGCCGGGGATATCCACCCGGCAAtgt             <  1:2265258/100‑1 (MQ=255)
                                                                                  cGTAAGAAAGCGCTGACTTATGAGCGTGAAGTGCTGGGCAAATTTGATAAAACTGCGGATCAATAATTGAAGATTGCCGGGGATATCCACCCGGCAAtct             <  2:182383/100‑3 (MQ=255)
                                                                                   gTAAGAAAGCGCTGACTTATGAGCGTGAAGTGCTGGGCAAATTTGATAAAACTGCGGATCAATAATTCAAGATTGCCGGGGATATCCACCCGGCAAtgtg            <  1:2896289/100‑1 (MQ=255)
                                                                                         aaGCGCTGACTTATGAGCGTGAAGTGCTGGGCAAATTTGATAAAACTGCGGATCAATAATTGAAGATTGCCGGGGATATCCACCCGGCAATGTGTGAATg      >  1:820285/1‑100 (MQ=255)
                                                                                           gcgcTGACTTATGAGCGTGAAGTGCTGGGCAAATTTGATAAAACTGCGGATCAATAATTGAAGATTGCCGGGGATATCCACCCGGCAATGTGTGAATGcc    <  2:2096261/100‑1 (MQ=255)
                                                                                            cgcTGACTTATGAGCGTGAAGTGCTGGGCAAATTTGATAAAACTGCGGATCAATAATTGAAGATTGCCGGGGATATCCACCCGGCAATGTGTGAATGCCt   >  1:2413879/1‑100 (MQ=255)
                                                                                            cgcTGACTTATGAGCGTGAAGTGCTGGGCAAATTTGATAAAACTGCGGATCAATAATTGAAGATTGCCGGGGATATCCACCCGGCAATGTGTGAATGCCt   <  2:16904/100‑1 (MQ=255)
                                                                                            cgcTGACTTATGAGCGTGAAGTGCTGGGAAAATTTGATAAAACTGCGGATCAATAATTGAAGATTGCCGGGGATATCCACCCGGCAATGTGTGAATGCCt   <  2:1017142/100‑1 (MQ=255)
                                                                                             gcTGACTTATGAGCGTGAAGTGCTGGGCAAATTTGATAAAACTGCGGATCAATAATTGAAGATTGCCGGGGATATCCACCCGGCAATGTGTGAATGCCTg  <  1:2468052/100‑1 (MQ=255)
                                                                                             gcTGACTTATGAGCGTGAAGTGCTGGGCAAATTTGATAAAACTGCGGATCAATAATTGAAGATTGCCGGGGATATCCACCCGGCAATGTGTGAATGCCTg  <  2:104729/100‑1 (MQ=255)
                                                                                                 |                                                                                               
CGACATGGCGCGTACTGCGCTGAACGTGGTGGGTAATGCGCTGGCGGTGCTGGTGATTGCCAAGTGGGAACACAAATTTGACCGTAAGAAAGCGCTGGCTTATGAGCGTGAAGTGCTGGGCAAATTTGATAAAACTGCGGATCAATAATTGAAGATTGCCGGGGATATCCACCCGGCAATGTGTGAATGCCTG  >  NC_000913/4295647‑4295839

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A