Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A4 F191 I0 R1 105 142.7 6924153 96.8% 6702580 99.9

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 4,295,744 G→A 59.7% A422T (GCT→ACT)  gltP → glutamate/aspartate:proton symporter

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,295,7440GA59.7% 92.3 / 173.5 147A422T (GCT→ACT) gltPglutamate/aspartate:proton symporter
Reads supporting (aligned to +/- strand):  ref base G (31/28);  new base A (40/47);  total (71/76)
Fisher's exact test for biased strand distribution p-value = 5.01e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 148 total aligned reads displayed.

CGACATGGCGCGTACTGCGCTGAACGTGGTGGGTAATGCGCTGGCGGTGCTGGTGATTGCCAAGTGGGAACACAAATTTGACCGTAAGAAAGCGCTGGCTTATGAGCGTGAAGTGCTGGGCAAATTTGATAAAACTGCGGATCAATAATTGAAGATTGCCGGGGATATCCACCCGGCAATGTGTGAATGCCTGATGC  >  NC_000913/4295647‑4295843
                                                                                                 |                                                                                                   
cGACATGGCGCGTACTGCGCTGAACGTGGTGGGTAATGCGCTGGCGGTGCTGGTGATTGCCAAGTGGGAACACAAATTTGACCGTAAGAAAGCGCTGACt                                                                                                   <  2:3080648/100‑1 (MQ=255)
cGACATGGCGCGTACTGCGCTGAACGTGGTAGGTAATGCGCTGGCGGTGCTGGTGATTGCCAAGTGGGAACACAAATTTGACCGTAAGAAAGCGCTGGCt                                                                                                   >  1:1247904/1‑100 (MQ=255)
 gACATGGCGCGTACTGCGCTGAACGTGGTGGGTAATGCGCTGGCGGTGCTGGTGATTGCCAAGTGGGAACACAAATTTGACCGTAAGAAAGCGCTGGCtt                                                                                                  >  1:1605389/1‑100 (MQ=255)
   cATGGCGGGTACTGCGCTGAACGTGGTGGGTAATGCGCTGGCGGTGCTGGTGATTGCCAAGTGGGAACACAAATTTGACCGTAAGAAAGCGCTGGCTTAt                                                                                                <  1:3154871/100‑1 (MQ=255)
    aTGGCGCGTACTGCGCTGAACGTGGTGGGTAATGCGCTGGCGGTGCTGGTGATTGCCAAGTGGGAACACAAATTTGACCGTAAGAAAGCGCTGACTTATg                                                                                               <  1:524439/100‑1 (MQ=255)
      ggCGCGTACTGCGCTGAACGTGGTGGGTAATGCGCTGGCGGTGCTGGTGATTGCCAAGTGGGAACACAAATTTGACCGTAAGAAAGCGCTGACTTATGAg                                                                                             <  2:3299330/100‑1 (MQ=255)
       gcgcGTACTGCGCTGAACGTGGTGGGTAATGCGCTGGCGGTGCTGGTGATTGCCAAGTGGGAACACAAATTTGACCGTAAGAAAGCGCTGGCTTATTAGc                                                                                            >  1:1947880/1‑100 (MQ=255)
        cgcgTACTGCGCTGAACGTGGTGGGTAATGCGCTGGCGGTGCTGGTGATTGCCAAGTGGGAACACAAATTTGACCGTAAGAAAGCGCTGGCTTATGAGCg                                                                                           <  2:888894/100‑1 (MQ=255)
          cgTACTGCGCTGAACGTGGTGGGTAATGCGCTGGCGGTGCTGGTGATTGCCAAGTGGGAACACAAATTTGACCGTAAGAAAGCGCTGACTTATGAGCGTg                                                                                         <  1:3295770/100‑1 (MQ=255)
           gTACTGCGCTGAACGTGGTGGGTAATGCGCTGGCGGGGCTGGTGATTGCCAAGTGGGAACACAAATTTGACCGTAAGAAAGCGCTGGCTTATGAGCGTGa                                                                                        <  2:371141/100‑1 (MQ=255)
              cTGCGCTGAACGTGGTGGGTAATGCGCTGGCGGTGCTGGTGATTGCCAAGTGGGAACACAAATTTGACCGTAAGAAAGCGCTGGCTTATGAGCGTGAAGt                                                                                     <  2:1605404/100‑1 (MQ=255)
                gcgcTGAACGTGGTGGGTAATGCGCTGGCGGTGCTGGTGATTGCCAAGTGGGAACACAAATTTGACCGTAAGAAAGCGCTGGCTTATGAGCGTGAAGTGc                                                                                   <  2:69461/100‑1 (MQ=255)
                gcgcTGAACGTGGTGGGGAATGCGCTGTCGGTGCGGGTGATTGCCAAGTGGGAACACAAATTTGACCGTAAGAAAGCGCTGGCTTATGAGCGTGAAGTGc                                                                                   >  1:1370573/1‑100 (MQ=255)
                   cTGAACGTGGTGGGTAATGCGCTGGCGGTGCTGGTGATTGCCAAGTGGGAACACAAATTTGACCGTAAGAAAGCGCTGACTTATGAGCGTGAAGTGCTgg                                                                                >  2:316563/1‑100 (MQ=255)
                   cTGAACGTGGTGGGTAATGCGCTGGCGGTGCTGGTGATTGCCAAGTGGGAACACAAATTTGACCGTAAGAAAGCGCTGACTTATGAGCGTGAAGTGCTgg                                                                                <  1:2069014/100‑1 (MQ=255)
                   cTGAACGTGGTGGGTAATGCGCTGGCGGTGCCGGTGATTGCCAAGTGGGAACACAAATTTGACCGTAAGAAAGCGCTGACTTATGAGCGTGAAGTGCTgg                                                                                >  2:2052959/1‑100 (MQ=255)
                    tGAACGTGGTGGGTAATGCGCTGGCGGTGCTGGTGATTGCCAAGTGGGAACACAAATTTGACCGTAAGAAAGCGCTGGCTTATGAGCGTGAAGTGCTggg                                                                               <  1:3135890/100‑1 (MQ=255)
                     gAACGTGGTGGGTAATGCGCTGGCGGTGCTGGTGATTGCCCAGTGGGAACACAAATTTGACCGTACGAAAGCGCAGGCTTATGAGCGTGAAGTGCTGGGc                                                                              >  1:3372317/1‑100 (MQ=255)
                     gAACGTGGTGGGTAATGCGCTGGCGGTGCTGGTGATTGCCAAGTGGGAACACAAATTTGACCGTAAGAAAGCGCTGACTTATCAGCGTGAAGTGCTGGGc                                                                              >  1:2751778/1‑100 (MQ=255)
                     gAACGTGGTCGGTAATGCGCTGGCGGTGCTGGTTATTGCCAAGTGGGAACACAAATTTGACCGTAAGAAAGCGCTGCCTTATGAGCGTGAAGTGCTgg                                                                                <  1:1472001/98‑1 (MQ=255)
                        cGTGGTGGGTAATGCGCTGGGGGTGCTGGTGATTGCCAAGTGGGAACACAAATTTGACCGTAAGAAAGCGCTGGCTTATGAGCGTGAAGTGCTGGGCaaa                                                                           >  2:694782/1‑100 (MQ=255)
                          tggtggGTAATGCGCTGGCGGTGCTGGTGATTGCCAAGTGGGAACACAAATTTGACCGTAAGAAAGCGCTGACTTATGAGCGTGAAGTGCTGGGCAAAtt                                                                         <  2:589699/100‑1 (MQ=255)
                          tggtggGTAATGCGCTGGCGGTGCTGGTGATTGCCAAGTGGGAACACAAATTTGACCGTAAGAAAGCGCTGACTTATGAGCGTGAAGTGCTGGGCAAAtt                                                                         <  1:1707693/100‑1 (MQ=255)
                          tggtggGTAATGCGCTGGCGGTGCTGGTGATTGCCAAGTGGGAACACAAATTTGACCGTAAGAAAGCGCTGACTTATGAGCGTGAAGTGCTGGGCAAAtt                                                                         <  1:2562571/100‑1 (MQ=255)
                           ggtggGTAATGCGCTGGCGGTGCTGGTGATTGCCAAGTGGGAACACAAATTTGACCGTAAGAAAGCGCTGGCTTATGAGCGTGAAGTGCTGGGCAAAttt                                                                        >  2:2922329/1‑100 (MQ=255)
                           ggtggGTAATGCGCTGGCGGTGCTGGTGATTGCCAAGTGGGAACACAAATTTGACCGTAAGAAAGCGCTGGCTTATGAGCGTGAAGTGCTGGGCAAAttt                                                                        <  1:2001365/100‑1 (MQ=255)
                           ggtggGTAATGCGCTGGCGGTGCTGGTGATTGCCAAGTGGGAACACAAATTTGACCGTAAGAAAGCGCTGGCTTATGAGCGTGAAGTGCTGGGCAAAttt                                                                        <  2:580031/100‑1 (MQ=255)
                            gtggGTAATGCGCTGGCGGTGCTGGTGATTGCCAAGTGGGAACACAAATTTGACCGTAAGAAAGCGCTGACTTATGAGCGTGAAGTGCTGGGCAAATTTg                                                                       <  2:1458613/100‑1 (MQ=255)
                             tggGTAATGCGCTGGCGGTGCTGGTGATTGCCAAGTGGGAACACAAATTTGACCGTAAGAAAGCGCTGACTTATGAGCGTGAAGTGCTGGGCAAATTTGa                                                                      >  1:948614/1‑100 (MQ=255)
                             tggGTAATGCGCTGGCGGTGCTGGTGATTGCCAAGTGGGAACACAAATTTGACCGTAAGAAAGCGCTGACTTATGAGCGTGAAGTGCTGGGCAAATTTGa                                                                      <  2:820948/100‑1 (MQ=255)
                               ggTAATGCGCTGGCGGTGCTGGTGATTGCCAAGTGGGAACACAAATTTGACCGTAAGAAAGCGCTGACTTATGAGCGTGAAGTGCTGGGCAAATTTGATa                                                                    <  2:2992576/100‑1 (MQ=255)
                               ggTAATGCGCTGGCGGTGCTGGTGATTGCCAAGTGGGAACACAAATTTGACCGTAAGAAAGCGCTGACTTATGAGCGTGAAGTGCTGGGCAAATTTGATa                                                                    <  1:1026930/100‑1 (MQ=255)
                                gTAATGCGCTGGCGGTGCTGGTGATTGCCAAGTGGGAACACAAATTTGACCGTAAGAAAGCGCTGGCTTATGAGCGTGAAGTGCTGGGCAAATTTGATaa                                                                   <  1:577894/100‑1 (MQ=255)
                                gTAATGCGCTGGCGGTGCTGGTGATTGCCAAGTGGGAACACAAATTTGACCGTAAGAAAGCGCTGACTTATGAGCGTGAAGTGCTGGGCAAATTTGATaa                                                                   >  1:2775244/1‑100 (MQ=255)
                                   aTGCGCTGGCGGTGCTGGTGATTGCCAAGTGGGAACACAAATTTGACCGTAAGAAAGCGCTGACTTATGAGCGTGAAGTGCTGGGCAAATTTGATAAAAc                                                                <  1:2981055/100‑1 (MQ=255)
                                    tGCGCTGGCGGTGCTGGTGATTGCCAAGTGGGAACACAAATTTGACCGTAAGAAAGCGCTGGCTTATGAGCGTGAAGTGCTGGGCAAATTTGATAAAACt                                                               >  1:2786572/1‑100 (MQ=255)
                                       gcTGGCGGTGCTGGTGATTGCCAAGTGGGAACACAAATTTGACCGTAAGAAAGCGCTGGCTTATGAGCGTGAAGTGCTGGGCAAATTTGATAAAACTGCg                                                            <  1:2672096/100‑1 (MQ=255)
                                       gcTGGCGGTGCTGGTGATTGCCAAGTGGGAACACAAATTTGACCGTAAGAAAGCGCAGGCTTATGAGCGTGAAGTGCTGGGCAAATTTGATAAAACTGCg                                                            >  1:2219648/1‑100 (MQ=255)
                                        cTGGCGGTGCTGGTGATTGCCAAGTGGGAACACAAATTTGACCGTAAGAAAGCGCTGACTTATGGGCGTGAAGTGCTGGGCAAATTTGATAAAACTGCgg                                                           <  1:3328350/100‑1 (MQ=255)
                                         tGGCGGTGCTGGTGATTGCCAAGTGGGAACACAAATTTGACCGTAAGAAAGCGCTGGCTTATGAGCGTGAAGTGCTGGGCAAATTTGATAAAACTGCgg                                                           <  1:346989/99‑1 (MQ=255)
                                         tGGCGGTGCTGGTGATTGCCAAGTGGGAACACAAATTTGACCGTAAGAAAGCGCTGGCTTATGAGCGTGAAGTGCTGGGCAAATTTGATAAAACTGCGGa                                                          <  2:72115/100‑1 (MQ=255)
                                           gCGGTGCTGGTGATTGCCAAGTGGGAACACAAATTTGACCGTAAGAAAGCGCTGGCTTATGAGCGTGAAGTGCTGGGCAAATTTGATAAAACTGCGGATc                                                        <  2:2444170/100‑1 (MQ=255)
                                           gCGGTGCTGGTGATTGCCAAGTGGGAACACAAATTTGACCGTAAGAAAGCGCTGACTTATGAGCGTGAAGTGCTGGGCAAATTTGATAAAACTGCGGATc                                                        <  1:2305067/100‑1 (MQ=255)
                                           gCGGTGCTGGTGATTGCCAAGTGGGAACACAAATTTGACCGTAAGAAAGCGCTGACTTATGAGCGTGAAGTGCTGGGCAAATTTGATAAAACTGCGGATc                                                        <  2:2853878/100‑1 (MQ=255)
                                            cGGTGCTGGTGATTGCCAAGTGGGAACACAAATTTGACCGTAAGAAAGCGCTGACTTATGAGCGTGAAGTGCTGGGCAAATTTGATAAAACTGCGGATCa                                                       <  2:1174958/100‑1 (MQ=255)
                                             ggTGCTGGTGATTGCCAAGTGGGAACACAAATTTGACCGTAAGAAAGCGCTGGCTTATGAGCGTGAAGTGCTGGGCAAATTTGATAAAACTGCGGATCaa                                                      <  1:3448057/100‑1 (MQ=255)
                                              gTGCTGGTGATTGCCAAGTGGGAACACAAATTTGACCGTAAGAAAGCGCTGGCTTATGAGCGTGAAGTGCTGGGCAAATTTGATAAAACTGCGGATCaat                                                     >  2:3252455/1‑100 (MQ=255)
                                              gTGCTGGTGATTGCCAAGTGGGAACACAAATTTGACCGTAAGAAAGCGCTGACTTATGAGCGTGAAGTGCTGGGCAAATTTGATAAAACTGCGGATCaat                                                     >  1:1784547/1‑100 (MQ=255)
                                               tGCTGGTGATTGCCAAGTGGGAACACAAATTTGACCGTAAGAAAGCGCTGGCTTATGAGCGTGAAGTGCTGGGCAAATTTGATAAAACTGCGGATCaata                                                    <  2:2603880/100‑1 (MQ=255)
                                                 cTGGTGATTGCCAAGTGGGAACACAAATTTGACCGTAAGAAAGCGCTGACTTATGAGCGTGAAGTGCTGGGCAAATTTGATAAAACTGCGGATCaataat                                                  >  1:1906319/1‑100 (MQ=255)
                                                 cTGGTGATTGCAAAGTGGGAACACAAATTTGACCGTAAGAAAGCGCTGACTTATGAGCGTGAAGTGCTGGGCAAATTTGATAAAACTGCGGATCaataat                                                  >  1:2658357/1‑100 (MQ=255)
                                                  tGGTGATTGCCAAGTGGGAACACAAATTTGACCGTAAGAAAGCGCTGGCTTATGAGCGTGAAGTGCTGGGCAAATTTGATAAAACTGCGGATCAATAAtt                                                 >  1:27503/1‑100 (MQ=255)
                                                  tGGTGATTGCCAAGTGGGAACACAAATTTGACCGTAAGAAAGCGCTGGCTTATGAGCGTGAAGTGCTGGGCAAATTTGATAAAACTGCGGATCAATAAtt                                                 >  2:1009908/1‑100 (MQ=255)
                                                  tGGTGATTGCCAAGTGGGAACACAAATTTGACCGTAAGAAAGCGCTGACTTATGAGCGTGAAGTGCTGGGCAAATTTGATAAAACTGCGGATCaataat                                                  >  1:2459012/1‑99 (MQ=255)
                                                  tGGTGATTGCCAAGTGGGAACACAAATTTGACCGTAAGAAAGCGCTGACTTATGAGCGTGAAGTGCTGGGCAAATTTGATAAAACTGCGGATCAATAAtt                                                 >  1:1477752/1‑100 (MQ=255)
                                                   ggTGATTGCCAAGTGGGAACACAAATTTGACCGTAAGAAAGCGCTGACTTATGAGCGTGAAGTGCTGGGCAAATTTGATAAAACTGCGGATCAATAATTg                                                >  1:18822/1‑100 (MQ=255)
                                                     tGATTGCCAAGTGGGAACACAAATTTGACCGTAAGAAAGCGCTGGCTTATGAGCGTGAAGTGCTGGGCAAATTTGATAAAACTGCGGATCAATAATTGaa                                              >  2:1796294/1‑100 (MQ=255)
                                                     tGATTGCCAAGTGGGAACACAAATTTGACCGTAAGAAAGCGCTGGCTTATGAGCGTGAAGTGCTAGGCAAATTTGATAAAACTGCGGATCAATAATTGaa                                              <  2:1370585/100‑1 (MQ=255)
                                                     tGATTGCCAAGTGGGAACACAAATTTGACCGTAAGAAAGCGCTGACTTATGAGCGTGAAGTGCTGGGCAAATTTGATAAAACTGCGGATCAATAATTGaa                                              <  1:2551428/100‑1 (MQ=255)
                                                      gATTGCCAAGTGGGAACACAAATTTGACCGTAAGAAAGCGCTGACTTATGAGCGTGAAGTGCTGGGCAAATTTGATAAAACTGCGGATCAATAATTGAAg                                             <  1:2030331/100‑1 (MQ=255)
                                                      gATTGCCAAGTGGGAACACAAATTTGACCGTAAGAAAGCGCTGACTTATGAGCGTGAAGTGCTGGGCAAATTTGATAAAACTGCGGATCAATAATTGAAg                                             >  1:227156/1‑100 (MQ=255)
                                                       aTTGCCAAGTGGGAACACAAATTTGACCGTAAGAAAGCGCTGGCTTATGAGCGTGAAGTGCTGGGCAAATTTGATAAAACTGCGGATCAATAATTGAAGa                                            >  1:3447001/1‑100 (MQ=255)
                                                        ttGCCAAGTGGGAACACAAATTTGACCGTAAGAAAGCGCTGACTTATGAGCGTGAAGTGCTGGGCAAATTTGATAAAACTGCGGATCAATAATTGAAGAt                                           <  2:1848850/100‑1 (MQ=255)
                                                         tGCCAAGTGGGAACACAAATTTGACCGTAAGAAAGCGCTGACTTATGAGCGTGAAGTGCTGGGCAAATTTGATAAAACTGCGGATCAATAATTGAAGAtt                                          >  1:2235625/1‑100 (MQ=255)
                                                         tGCCAAGTGGGAACACAAATTTGACCGTAAGAAAGCGCTGACTTATGAGCGTGAAGTGCTGGGCAAATTTGATAAAACTGCGGATCAATAATTGAAGAtt                                          <  2:2870902/100‑1 (MQ=255)
                                                         tGCCAAGTGGGAACACAAATTTGACCGTAAGAAAGCGCTGACTTATGAGCGTGAAGTGCTGGGCAAATTTGATAAAACTGCGGATCAATAATTGAAGAtt                                          >  1:2970014/1‑100 (MQ=255)
                                                            cAAGTGGGAACACAAATTTGACCGTAAGAAAGCGCTGACTTATGAGCGTGAAGTGCTGGGCAAATTTGATAAAACTGCGGATCAATAATTGAAGATTGcc                                       >  1:2409980/1‑100 (MQ=255)
                                                             aaGTGGGAACACAAATTTGACCGTAAGAAAGCGCTGACTTATGAGCGTGAAGTGCTGGGCAAATTTGATAAAACTGCGGATCAATAATTGAAGATTGCCg                                      >  1:704160/1‑100 (MQ=255)
                                                              aGTGGGAACACAAATTTGACCGTAAGAAAGCGCTGACTTATGAGCGTGAAGTGCTGGGCAAATTTGATAAAACTGCGGATCAATAATTGAAGATTGcctg                                     <  2:3199473/100‑3 (MQ=255)
                                                              aGTGGGAACACAAATTTGACCGTAAGAAAGCGCTGACTTATGAGCGTGAAGTGCTGGGCAAATTTGATAAAACTGCGGATCAATAATTGAAGATTGCCgg                                     <  2:2793304/100‑1 (MQ=255)
                                                                tGGGAACACAAATTTGACCGTAAGAAAGCGCTGACTTATGAGCGTGAAGTGCTGGGCAAATTTGATAAAACTGCGGATCAATAATTGAAGATTGCCgggg                                   <  1:1866916/100‑1 (MQ=255)
                                                                tGGGAACACAAATTTGACCGTAAGAAAGCGCTGACTTATGAGCGTGAAGTGCTGGGCAAATTTGATAAAACTGCGGATCAATAATTGAAGATTGCCgggg                                   <  2:1146470/100‑1 (MQ=255)
                                                                  ggAACACAAATTTGACCGTAAGAAAGCGCTGGCTTATGAGCGTGAAGTGCTGGGCAAATTTGATAAAACTGCGGATCAATAATTGAAGATTGCCGGGGa                                  <  1:2046964/99‑1 (MQ=255)
                                                                   gAACACAAATTTGACCGTAAGAAAGCGCTGACTTATGAGCGTGAAGTGCTGGGCAAATTTGATAAAACTGCGGATCAATAATTGAAGATTGCCGGGGata                                <  2:722270/100‑1 (MQ=255)
                                                                     acacAAATTTGACCGTAAGAAAGCGCTGACTTATGAGCGTGAAGTGCTGGGCAAATTTGATAAAACTGCGGATCAATAATTGAAGATTGCCGGGGATATc                              >  2:2657008/1‑100 (MQ=255)
                                                                      cacaAATTTGACCGTAAGAAAGCGCTGGCTTATGAGCGTGAAGTGCTGGGCAAATTTGATAAAACTGCGGATCAATAATTGAAGATTGCCGGGGATATcc                             >  1:3234449/1‑100 (MQ=255)
                                                                        caAATTTGACCGTAAGAAAGCGCTGGCTTATGAGCGTGAAGTGCTGGGCAAATTTGATAAAACTGCGGATCAATAATTGAAGATTGCCGGGGATATCCAc                           >  2:1993287/1‑100 (MQ=255)
                                                                        caAATTTGACCGTAAGAAAGCGCTGACTTATGAGCGTGAAGTGCTGGGCAAATTTGATAAAACTGCGGATCAATAATTGAAGATTGCCGGGGATATCCAc                           >  2:2711355/1‑100 (MQ=255)
                                                                         aaaTTTGACCGTAAGAAAGCGCTGGCTTATGAGCGTGAAGTGCTGGGCAAATTTGATAAAACTGCGGATCAATAATTGAAGATTGCCGGGGATATCCAcc                          <  2:44148/100‑1 (MQ=255)
                                                                            tttGACCGTAAGAAAGCGCTGACTTATGAGCGTGAAGTGCTGGGCAAATTTGATAAAACTGCGGATCAATAATTGAAGATTGCCGGGGATATCCACCCgg                       >  2:1693161/1‑100 (MQ=255)
                                                                            tttGACAGTAAGAAAGCGCTGGCTTATGATCGTGAAGTGCTGGGCAAATTTGATAAAACTGCGGATCAATAATTGAAGATTGCCGGGGATATCCACCCgg                       <  1:477314/100‑1 (MQ=255)
                                                                             ttGACCGTAAGAAAGCGCTGACTTATGAGCGTGAAGTGCTGGGCAAATTTGATAAAACTGCGGATCAATAATTGAAGATTGCCGGGGATATCCACCCGGc                      <  2:1241230/100‑1 (MQ=255)
                                                                              tGACCGTAAGAAAGCGCTGACTTATGAGCGTGAAGTGCTGGGCAAATTTGATAAAACTGCGGATCAATAATTGAAGATTGCCGGGGATATCCACCCGGCa                     >  2:1301800/1‑100 (MQ=255)
                                                                              tGACCGTAAGAAAGCGCTGACTTATGAGCGTGAAGTGCTGGGCAAATTTGATAAAACTGCGGATCAATAATTGAAGATTGCCGGGGATATCCACCCGGCa                     >  1:1753725/1‑100 (MQ=255)
                                                                               gACCGTAAGAAAGCGCTGACTTATGAGCGTGAAGTGCTGGGCAAATTTGATAAAACTGCGGATCAATAATTGAAGATTGCCGGGGATATCCACCCGGCaa                    >  2:2154848/1‑100 (MQ=255)
                                                                                aCCGTAAGAAAGCGCTGACTTATGAGCGTGAAGTGCTGGGCAAATTTGATAAAACTGCGGATCAATAACTGAAGATTGCCGGGGATATCCACCCGGCAAt                   >  1:55204/1‑100 (MQ=255)
                                                                                 ccGTAAGAAAGCGCTGGCTTATGAGCGTGAAGTGCTGGGCAAATTTGATAAAACTGCGGATCAATAATTGAAGATTGCCGGGGATATCCACCCGGCAAtg                  >  1:807636/1‑100 (MQ=255)
                                                                                 ccGTAAGAAAGCGCTGACTTATGAGCGTGAAGTGCTGGGCAAATTTGATAAAACTGCGGATCAATAATTGAAGATTGCCGGGGATATCCACCCGGCAAtg                  >  2:2671478/1‑100 (MQ=255)
                                                                                     aaGAAAGCGCTGACTTATGAGCGTGAAGTGCTGGGCAAATTTGATAAAACTGCGGATCAATAATTGAAGATTGCCGGGGATATCCACCCGGCAAtgtgtg              <  2:1136810/100‑1 (MQ=255)
                                                                                       gAAAGCGCTGGCTTATGAGCGTGAAGTGCTGGGCAAATTTGATAAAACTGCGGATCAATAATTGAAGATTGCCGGGGATATCCACCCGGCAATGTGTGaa            >  1:2453578/1‑100 (MQ=255)
                                                                                       gAAAGCGCTGACTTATGAGCGTGAAGTGCTGGGCAAATTTGATAAAACTGCGGATCAATAATTGAAGATTGCCGGGGATATCCACCCGGCAATGTGTGaa            <  2:2221239/100‑1 (MQ=255)
                                                                                           gcgcTGACTTATGAGCGTGAAGTGCTGGGCAAATTTGATAAAACTGCGGATCAATAATTGAAGATTGCCGGGGATATCCACCCGGCAATGTGTGAATGcc        >  1:2161562/1‑100 (MQ=255)
                                                                                           gcgcTGACTTATGAGCGTGAAGTGCTGGGCAAATTTGATAAAACTGCGGATCAATAATTGAAGATTGCCGGGGATATCCACCCGGCAATGTGTGAATGcc        >  2:1107794/1‑100 (MQ=255)
                                                                                           gcgcAGACTTATGACGATAAAGTGCTGGGCAAATTTGATAAAACTGCGGAGCAATAATTGAAGATTACCGGAGATATCCACCCGGCAATGTGTGAATGcc        <  1:1968823/100‑1 (MQ=255)
                                                                                            cgcTGACTTATGAGCGTGAAGTGCTGGGCAAATTTGATAAAACTGCGGATCAATAATTGAAGATTGCCGGGGATATCCACCCGGCAATGTGTGAATGCCt       <  1:1112402/100‑1 (MQ=255)
                                                                                             gcTGGCTTATGAGCGTGAAGTGCTGGGCAAATTTGATAAAACTGCGGATCAATAATTGAAGATTGCCGGGGATATCCACCCGGCAATGTGTGAATGCCTg      <  2:2219666/100‑1 (MQ=255)
                                                                                             gcTGACTTATGAGCGTGAAGTGCTGGGCAAATTTGATAAAACTGCGGATCAATAATTGAAGATTGCCGGGGATATCCACCCGGCAATGTGTGAATGCCTg      <  2:227158/100‑1 (MQ=255)
                                                                                              cTGGCTTATGAGCGTGAAGTGCTGGGCAAATTTGATAAAACTGCGGATCAATAATTGAAGATTGCCGGGGATATCCACCCGGCAATGTGTGAATGCCTGa     >  1:1335320/1‑100 (MQ=255)
                                                                                              cTGGCTTATGAGCGTGAAGTGCTGGGCAAATTTGATAAAACTGCGGATCAATAATTGAAGATTGCCGGGGATATCCACCCGGCAATGTGTGAATGCCTGa     <  2:2786594/100‑1 (MQ=255)
                                                                                                 gCTTATGAGCGTGAAGTGCTGGGCAAATTTGATAAAACTGCGGATCAATAATTGAAGATTGCCGGGGATATCCACCCGGCAATGTGTGAATGCCTGATGc  <  2:3372341/100‑1 (MQ=255)
                                                                                                 |                                                                                                   
CGACATGGCGCGTACTGCGCTGAACGTGGTGGGTAATGCGCTGGCGGTGCTGGTGATTGCCAAGTGGGAACACAAATTTGACCGTAAGAAAGCGCTGGCTTATGAGCGTGAAGTGCTGGGCAAATTTGATAAAACTGCGGATCAATAATTGAAGATTGCCGGGGATATCCACCCGGCAATGTGTGAATGCCTGATGC  >  NC_000913/4295647‑4295843

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A