Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A16 F201 I0 R1 306 176.9 8831342 93.4% 8248473 99.8

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 4,295,744 G→A 25.4% A422T (GCT→ACT)  gltP → glutamate/aspartate:proton symporter

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,295,7440GA25.4% 376.1 / 128.1 208A422T (GCT→ACT) gltPglutamate/aspartate:proton symporter
Reads supporting (aligned to +/- strand):  ref base G (76/78);  new base A (32/21);  total (108/100)
Fisher's exact test for biased strand distribution p-value = 2.03e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.97e-01

Only 100 of 216 total aligned reads displayed.

CTCGACATGGCGCGTACTGCGCTGAACGTGGTGGGTAATGCGCTGGCGGTGCTGGTGATTGCCAAGTGGGAACACAAATTTGACCGTAAGAAAGCGCTGGCTTATGAGCGTGAAGTGCTGGGCAAATTTGATAAAACTGCGGATCAATAATTGAAGATTGCCGG‑‑GGATATCCACCCGGCAATGTGTGAATGCCTGATGC  >  NC_000913/4295645‑4295843
                                                                                                   |                                                                                                     
cTCGACATGGCGCGTACTGCGCTGAACGTGGTGGGTAATGCGCTGGCGGTGCTGGTGATTGCCAAGTGGGAACACAAATTTGACCGTAAGAAAGCGCTgg                                                                                                       <  2:1153940/100‑1 (MQ=255)
cTCGACATGGCGCGTACTGCGCTGAACGTGGTGGGTAATGCGCTGGCGGTGCTGGTGATTGCCAAGTGGGAACACAAATTTGACCGTAAGAAAGCGCTgg                                                                                                       >  1:179206/1‑100 (MQ=255)
  tccgatctcgcgTACTGCGCTGAACGTGGTGGGTAATGCGCTGGCGGTGCTGGTGATTGCCAAGTGGGAACACAAATTTGACCGTAAGAAAGCGCTGGCt                                                                                                     <  1:3104097/92‑1 (MQ=255)
  cGACATGGCGCGTACTGCGCTGAACGTGGTGGGTAATGCGCTGGCGGTGCTGGTGATTGCCAAGTGGGAACACAAATTTGACCGTAAGAAAGCGCTGGCt                                                                                                     <  2:2613172/100‑1 (MQ=255)
  cGACATGGCGCGTACTGCGCTGAACGCGGTGGGTAATGCGCTGGCGGTGCTGGTGATTGCCAAGTGGTAACACAAATTTGACCGTAAGAAAGCGCTGGc                                                                                                      <  1:2517729/99‑1 (MQ=255)
      aTGGCGCGTACTGCGCTGAACGTGGTGGGTAATGCGCTGGCGGTGCTGGTGATTGCCAAGTGGGAACACAAATTTGACCGTAAGAAAGCGCTGGCTTATg                                                                                                 <  1:777455/100‑1 (MQ=255)
          cgcgTACTGCGCTGAACGTGGTGGGTAATGCGCTGGCGGTGCTGGTGATTGCCAAGTGGGAACACAAATTTGACCGTAAGAAAGCGCTGGCtagatcgga                                                                                             >  2:3104115/1‑92 (MQ=255)
          cgcgTACTGCGCTGAACGTGGTGGGTAATGCGCTGGCGGTGCTGGTGATTGCCAAGTGGGAACACAAATTTGACCGTAAGAAAGCGCTGGCTTATGAGCg                                                                                             <  1:305433/100‑1 (MQ=255)
          cgcgTACTGCGCTGAACGTGGTGGGTAATGCGCTGGCGGTGCTGGTGATTGCCAAGTGGGAACACAAATTTGACCGTAAGAAAGCGCTGGCTTATGAGCg                                                                                             <  1:3915822/100‑1 (MQ=255)
          cgcgTACTGCGCTGAACGTGGTGGGTAATGCGCTGGCGGTGCTGGTGATTGCCAAGTGGGAACACAAATTTGAACGTAAGAAAGCGCTGGCTTATGAGCg                                                                                             >  1:200193/1‑100 (MQ=255)
           gcgTACTGCGCTGAACGTGGTGGGTAATGCGCTGGCGGTGCTGGTGATTGCCAAGTGGGAACACAAATTTGACCGTAAGAAAGCGCTGGCTTATGAGCg                                                                                             <  1:581517/99‑1 (MQ=255)
           gcgTACTGCGCTGAACGTGGTGGGTAATGCGCTGGCGGTGCTGGTGATTGCCAAGTGGGAACACAAATTTGACCGTAAGAAAGCGCTGGCTTATGAGCGt                                                                                            <  1:1692724/100‑1 (MQ=255)
            cgTACTGCGCTGAACGTGGTGGGTAATGCGCTGGCGGTGCTGGTGATTGCCAAGTGGGAACACAAATTTGACCGTAAGAAAGCGCTGGCTTATGAGCGTg                                                                                           <  2:2054513/100‑1 (MQ=255)
              tACTGCGCTGAACGTGGTGGGTAATGCGCTGGCGGTGCTGGTGATTGCCAAGTGGGAACACAAATTTGACCGTAAGAAAGCGCTGACTTATGAGCGTGaa                                                                                         >  2:3968031/1‑100 (MQ=255)
               aCTGCGCTGAACGTGGTGGGTAATGCGCTGGCGGTGCTGGTGATTGCCAAGTGGGAACACAAATTTGACCGTAAGAAAGCGCTGGCTTATGAGCGTGAAg                                                                                        <  2:1003980/100‑1 (MQ=255)
               aCTGCGCTGAACGTGGTGGGTAATGCGCTGGCGGTGCTGGTGATTGCCAAGTGGGAACACAAATTTGACCGTAAGAAAGCGCTGGCTTATGAGCGTGAAg                                                                                        <  2:1420201/100‑1 (MQ=255)
                cTGCGCTGAACGTGGTGGGTAATGCGCTGGCGGTGCTGGTGATTGCCAAGTGGGAACACAAATTTGACCGTAAGAAAGCGCTGGCTTATGAGCGTGAAGt                                                                                       >  1:3871185/1‑100 (MQ=255)
                 tGCGCTGAACGTGGTGGGTAATGCGCTGGCGGTGCTGGTGATTGCCAAGTGGGAACACAAATTTGACCGTAAGAAAGCGCTGGCTTATGAGCGTGAAGTg                                                                                      >  1:4020292/1‑100 (MQ=255)
                  gcgcTGAACGTGGTGGGTAATGCGCTGGCGGTGCTGGTGATTGCCAAGTGGGAACACAAATTTGACCGTAAGAAAGCGCTGGCTTATGAGCGTGAAGTGc                                                                                     >  1:3606066/1‑100 (MQ=255)
                  gcgcTGAACGTGGTGGGTAATGCGCTGGCGGTGCTGGTGATTGCCAAGTGGGAACACAAATTTGACCGTAAGAAAGCGCTGACTTATGAGCGTGAAGTg                                                                                      >  2:3892684/1‑99 (MQ=255)
                  gcgcTGAACGTGGTGGGTAATGCGCTGGCGGTGCTGGTGATTGCCAAGTGGGAACACAAATTTGACCGTAAGAAAGCGCTGACTTATGAGCGTGAAGTGc                                                                                     >  1:3291522/1‑100 (MQ=255)
                   cgcTGAACGTGGTGGGTAATGCGCTGGCGGTGCTGGTGATTGCCAAGTGGGAACACAAATTTGACCGTAAGAAAGCGCTGGCTTATGAGCGTGAAGTGCt                                                                                    >  1:1080942/1‑100 (MQ=255)
                    gcTGAACGTGGTGGGTAATGCGCTGGCGGTGCTGGTGATTGCCAAGTGGGAACACAAATTTGACCGTAAGAAAGCGCTGGCTTATGAGCGTGAAGTGCTg                                                                                   >  1:90611/1‑100 (MQ=255)
                     cTGAACGTGGTGGGTAATGCGCTGGCGGTGCTGGTGATTGCCAAGTGGGAACACAAATTTGACCGTAAGAAAGCGCTGGCTTATGAGCGTGAAGTGCTgg                                                                                  <  2:2773487/100‑1 (MQ=255)
                     cTGAACGTGGTGGGTAATGCGCTGGCGGTGCTGGTGATTGCCAAGTGGGAACACAAATTTGACCGTAAGAAAGCGCTGGCTTATGAGCGTGAAGTGCTgg                                                                                  <  1:4325237/100‑1 (MQ=255)
                      tGAACGTGGTGGGTAATGCGCTGGCGGTGCTGGTGATTGCCAAGTGGGAACACAAATTTGACCGTAAGAAAGCGCTGACTTATGAGCGTGAAGTGCTggg                                                                                 >  2:1739744/1‑100 (MQ=255)
                       gAACGTGGTGGGTAATGCGCTGGCGGTGCTGGTGATTGCCAAGTGGGAACACAAATTTGACCGTAAGAAAGCGCTGGCTTATGAGCGTGAAGTGCTGGGc                                                                                >  1:2421469/1‑100 (MQ=255)
                         aCGTGGTGGGTAATGCGCTGGCGGTGCTGGTGATTGCCAAGTGGGAACACAAATTTGACCGTAAGAAAGCGCTGGCTTATGAGCGTGAAGTGCTGGGCaa                                                                              >  2:764061/1‑100 (MQ=255)
                            tggtggGTAATGCGCTGGCGGTGCTGGTGATTGCCAAGTGGGAACACAAATGTGACCGTAAGAAAGCGCTGACTTATGAGCGTGAAGTGCTGGGCAAAtt                                                                           <  1:1199986/100‑1 (MQ=255)
                             ggtggGTAATGCGCTGGCGGTGCTGGTGATTGCCAAGTGGGAACACAAATTTGACCGTAAGAAAGCGCTGGCTTATGAGCGTGAAGTGCTGGGCAAAttt                                                                          >  2:1031694/1‑100 (MQ=255)
                             ggtggGTAATGCGCTGGCGGTGCTGGTGATTGCCAAGTGGGAACACAAATTTGACCGTAAGAAAGCGCTGACTTATGAGCGTGAAGTGCTGGGCAAAttt                                                                          <  2:2354195/100‑1 (MQ=255)
                              gtggGTAATGCGCTGGCGGTGCTGGTGATTGCCAAGTGGGAACACAAATTTGACCGTAAGAAAGCGCTGGCTTATGAGCGTGAAGTGCTGGGCAAATTTg                                                                         >  2:1297722/1‑100 (MQ=255)
                               tggGTAATGCGCTGGCGGTGCTGGTGATTGCCAAGTGGGAACACAAATTTGACCGTAAGAAAGCGCTGGCTTATGAGCGTGAAGTGCTGGGCAAATTTGa                                                                        <  2:3955436/100‑1 (MQ=255)
                                  gTAATGCGCTGGCGGTGCTGGTGATTGCCAAGTGGGAACACAAATTTGACCGTAAGAAAGCGCTGGCTTATGAGCGTGAAGTGCTGGGCAAATTTGATaa                                                                     >  1:2163469/1‑100 (MQ=255)
                                  gTAATGCGCTGGCGGTGCTGGTGATTGCCAAGTGGGAACACAAATTTGACCGTAAGAAAGCGCTGACTTATGAGCGTGAAGTGCTGGGCAAATTTGATaa                                                                     >  1:3333400/1‑100 (MQ=255)
                                     aTGCGCTGGCGGTGCTGGTGATTGCCAAGTGGGAACACAAATTTGACCGTAAGAAAGCGCTGGCTTATGAGCGTGAAGTGCTGGGCAAATTTGATAAAAc                                                                  <  2:2759083/100‑1 (MQ=255)
                                     aTGCGCTGGCGGTGCTGGTGATTGCCAAGTGGGAACACAAATTTGACCGTAAGAAAGCGCTGGCTTATGAGCGTGAAGTGCTGGGCAAATTTGATAAAAc                                                                  <  2:3743949/100‑1 (MQ=255)
                                     aTGCGCTGGCGGTGCTGGTGATTGCCAAGTGGGAACACAAATGTGACCGTAAGAAAGCGCTGACTTATGAGCGTGAAGTGCTGGGCAAATTTGATaaaa                                                                   >  1:1029408/1‑99 (MQ=255)
                                      tGCGCTGGCGGTGCTGGTGATTGCCAAGTGGGAACACAAATTTGACCGTAAGAAAGCGCTGGCTTATGAGCGTGAAGTGCTGGGCAAATTTGATAAAACt                                                                 >  1:2039508/1‑100 (MQ=255)
                                      tGCGCTGGCGGTGCTGGTGATTGCCAAGTGGGAACACAAATTTGACCGTAAGAAAGCGCTGGCTTATGAGCGTGAAGTGCTGAGCAAATTTGATAAAAc                                                                  >  1:3320988/1‑99 (MQ=255)
                                            ggCGGTGCTGGTGATTGCCAAGTGGGAACACAAATTTGACCGTAAGAAAGCGCTGGCTTATGAGCGTGAAGTGCTGGGCAAATTTGATAAAACTGCGGAt                                                           >  2:1652747/1‑100 (MQ=255)
                                            ggCGGTGCTGGTGATTGCCAAGTGGGAACACAAATTTGACCGTAAGAAAGCGCTGGCTTATGAGCGTGAAGTGCTGGGCAAATTTGATAAAACTGCGGAt                                                           >  2:1580340/1‑100 (MQ=255)
                                             gCGGTGCTGGTGATTGCCAAGTGGGAACACAAATTTGACCGTAAGAAAGCGCTGGCTTATGAGCGTGAAGTGCTGGGCAAATTTGATAAAACTGCGGATc                                                          >  2:1084402/1‑100 (MQ=255)
                                             gCGGTGCTGGTGATTGCCAAGTGGGAACACAAATTTGACCGTAAGAAAGCGCTGGCTTATGAGCGTGAAGTGCTGGGCAAATTTGATAAAACTGCGGATc                                                          <  2:3291069/100‑1 (MQ=255)
                                             gCGGTGCTGGTGATTGCCAAGTGGGAACACAAATTTGACCGTAAGAAAGCGCTGGCTTATGAGCGTGAAGTGCTGGGCAAATTTGATAAAACTGCGGATc                                                          <  2:2818815/100‑1 (MQ=255)
                                              cGGTGCTGGTGATTGCCAAGTGGGAACACAAATTTGACCGTAAGAAAGCGCTGGCTTATGAGCGTGAAGTGCTGGGCAAATTTGATAAAACTGCGGATCa                                                         >  2:2276826/1‑100 (MQ=255)
                                               ggTGCTGGTGATTGCCAAGTGGGAACACAAATTTGACCGTAAGAAAGCGCTGGCTTATGAGCGTGAAGTGCTGGGCAAATTTGATAAAACTGCGGATCaa                                                        <  1:4156756/100‑1 (MQ=255)
                                                gTGCTGGTGATTGCCAAGTGGGAACACAAATTTGACCGTAAGAAAGCGCTGACTTATGAGCGTGAAGTGCTGGGCAAATTTGATAAAACTGCGGATCaat                                                       <  2:3794154/100‑1 (MQ=255)
                                                   cTGGTGATTGCCAAGTGGGAACACAAATTTGACCGTAAGAAAGCGCTGGCTTATGAGCGTGAAGTGCTGGGCAAATTTGATAAAACTGCGGATCaataat                                                    >  1:3559804/1‑100 (MQ=255)
                                                   cTGGTGATTGCCAAGTGGGAACACAAATTTGACCGTAAGAAAGCGCTGACTTATGAGCGTGAAGTGCTGGGCAAATTTGATAAAACTGCGGATCaataat                                                    >  2:558579/1‑100 (MQ=255)
                                                   cTGGTGATTGCCAAGTGGGAACACAAATTTGACCGTAAGAAAGCGCTGACTTATGAGCGTGAAGTGCTGGGCAAATTTGATAAAACTGCGGATCaataat                                                    <  1:1316963/100‑1 (MQ=255)
                                                    tGGTGATTGCCAAGTGGGAACACAAATTTGACCGTAAGAAAGCGCTGGCTTATGAGCGTGAAGTGCTGGGCAAATTTGATAAAACTGCGGATCAATAAtt                                                   >  1:36470/1‑100 (MQ=255)
                                                    tGGTGATTGCCAAGTGGGAACACAAATTTGACCGTAAGAAAGCGCTGGCTTATGAGCGTGAAGTGCTGGGCAAATTTGATAAAACTGCGGATCAATAAtt                                                   <  2:1763800/100‑1 (MQ=255)
                                                     ggTGATTGCCAAGTGGGAACACAAATTTGACCGTAAGAAAGCGCTGGCTTATGAGCGTGAAGTGCTGGGCAAATTTGATAAAACTGCGGATCAATAATTg                                                  >  2:3640659/1‑100 (MQ=255)
                                                     ggTGATTGCCAAGTGGGAACACAAATTTGACCGTAAGAAAGCGCTGGCTTATGAGCGTGAAGTGCTGGGCAAATTTGATAAAACTGCGGATCAATAATTg                                                  <  1:3497839/100‑1 (MQ=255)
                                                     ggTGATTGCCAAGTGGGAACACAAATTTGACCGTAAGAAAGCGCTGGCTTATGAGCGTGAAGTGCTGGGCAAATTTGATAAAACTGCGGATCAATAATTg                                                  >  2:2440935/1‑100 (MQ=255)
                                                      gTGATTGCCAAGTGGGAACACAAATTTGACCGTAAGAAAGCGCTGACTTATGAGCGTGAAGTGCTGGGCAAATTTGATAAAACTGCGGATCAATAATTGa                                                 <  1:3393978/100‑1 (MQ=255)
                                                       tGATTGCCAAGTGGGAACACAAATTTGACCGTAAGAAAGCGCTGGCTTATGAGCGTGAAGTGCTGGGCAAATTTGATAAAACTGCGGATCAATAATTGaa                                                <  1:2803245/100‑1 (MQ=255)
                                                       tGATTGCCAAGTGGGAACACAAATTTGACCGTAAGAAAGCGCTGGCTTATGAGCGTGAAGTGCTGGGCAAATTTGATAAAACTGCGGATCAATAATTGaa                                                >  2:702536/1‑100 (MQ=255)
                                                       tGATTGCCAAGTGGGAACACAAATTTGACCGTAAGAAAGCGCTGGCTTATGAGCGTGAAGTGCTGGGCAAATTTGATAAAACTGCGGATCAATAATTGaa                                                <  1:465777/100‑1 (MQ=255)
                                                        gATTGCCAAGTGGGAACACAAATTTGACCGTAAGAAAGCGCTGGCTTATGAGCGTGAAGTGCTGGGCAAATTTGATAAAACTGCGGATCAATAATTGAAg                                               >  2:3458905/1‑100 (MQ=255)
                                                            gCCAAGTTGGAACACAAATTTGACCGTAAGAAAGCGCTGGCTTATGAGCGTGAAGTGCTGGGCAAATTTGATAAAACTGCGGATCAATAATTGAAGATTg                                           >  2:2318377/1‑100 (MQ=255)
                                                            gCCAAGTGGGAACACAAATTTGACCGTAAGAAAGCGCTGACTTATGAGCGTGAAGTGCTGGGCAAATTTGATAAAACTGCGGATCAATAATTGAAGATTg                                           >  2:1439984/1‑100 (MQ=255)
                                                             ccAAGTGGGAACACAAATTTGACCGTAAGAAAGCGCTGGCTTATGAGCGTGAAGTGCTGGGCAAATTTGATAAAACTGCGGATCAATAATTGAAGATTGc                                          >  2:118099/1‑100 (MQ=255)
                                                             ccAAGTGGGAACACAAATTTGACCGTAAGAAAGCGCTGACTTATGAGCGTGAAGTGCTGGGCAAATTTGATAAAACTGCGGATCAATAATTGAAGATTGc                                          >  2:2954785/1‑100 (MQ=255)
                                                              cAAGTGGGAACACAAATTTGACCGTAAGAAAGCGCTGGCTTATGAGCGTGAAGTGCTGGGCAAATTTGATAAAACTGCGGATCAATAATTGAAGATTGcc                                         >  2:1778136/1‑100 (MQ=255)
                                                              cAAGTGGGAACACAAATTTGACCGTAAGAAAGCGCTGGCTTATGAGCGTGAAGTGCTGGGCAAATTTGATAAAACTGCGGATCAATAATTGAAGATTGcc                                         >  2:2628913/1‑100 (MQ=255)
                                                                 gggtgAACACAAATTTGACCGTAAGAAATAGCTGACTTATGAGCCTGAAGTGCTGGGCAAATTTGATAAAACTGCGGATCAATAATTGAAGATTGCCgg‑‑g                                    <  1:1752830/96‑1 (MQ=255)
                                                                 gTGGGAACACAAATTTGACCGTAAGAAAGCGCTGACTTATGAGCGTGAAGTGCTGGGCAAATTTGATAAAACTGCGGATCAATAATTGAAGATTGCCgg‑‑g                                    <  1:4292883/100‑1 (MQ=255)
                                                                  tGGGAACACAAATTTGACCGTAAGAAAGCGCTGGCTTATGAGCGTGAAGTGCTGGGCAAATTTGATAAAACTGCGGATCAATAATTGAAGATTGCCgg‑‑gg                                   <  2:1888960/100‑1 (MQ=255)
                                                                  tGGGAACACAAATTTGACCGTAAGAAAGCGCTGACTTATGAGCGTGAAGTGCTGGGCAAATTTGATAAAACTGCGGATCAATAATTGAAGATTGCCgg‑‑gg                                   <  2:4105365/100‑1 (MQ=255)
                                                                   nggAACACAAATTTGACCGTAAGAAAGCGCTGACTTATGAGCGTGAAGTGCTGGGCAAATTTGATAAAACTGCGGATCAATAATTGAAGATTGCCGG‑‑GGa                                  >  2:2404438/2‑100 (MQ=255)
                                                                   gcgaacggaaaTTGGACCGTACGATAGTGGTGTCTTATGAGCGTGAAGTTCTGGGCAAATGTGATAAAACTGCGGATCAATAATTGAAGATTGCCgg‑‑gg                                   <  1:3712523/91‑1 (MQ=255)
                                                                    ggAACACAAATTTGACCGTAAGAAAGCGCTGGCTTATGAGCGTGAAGTGCTGGGCAAATTTGATAAAACTGCGGATCAATAATTGAAGATTGCCGG‑‑GGat                                 >  1:3430344/1‑100 (MQ=255)
                                                                    ggAACACAAATTTGACCGTAAGAAAGCGCTGGCTTATGAGCGTGAAGTGCTGGGCAAATTTGATAAAACTGCGGATCAATAATTGAAGATTGCCGG‑‑GGat                                 <  1:665812/100‑1 (MQ=255)
                                                                      aaCACAAATTTGACCGTAAGAAAGCGCTGACTTATGAGCGTGAAGTGCTGGGCAAATTTGATAAAACTGCGGATCAATAATTGAAGATTGCCGG‑‑GGatat                               >  1:1213383/1‑100 (MQ=255)
                                                                       acacAAATTTGACCGTAAGAAAGCGCTGGCTTATGAGCGTGAAGTGCTGGGCAAATTTGATAAAACTGCGGATCAATAATTGAAGATTGCCGG‑‑GGATATc                              <  2:291540/100‑1 (MQ=255)
                                                                        cacaAATTTGACCGTAAGAAAGCGCTGGCTTATGAGCGTGAAGTGCTGGGCAAATTTGATAAAACTGCGGATCAATAATTGAAGATTGCCGG‑‑GGATATcc                             >  2:3531654/1‑100 (MQ=255)
                                                                         acaAATTTGACCGTAAGAAAGCGCTGGCTTATGAGCGTGAAGTGCTGGGCAAATTTGATAAAACTGCGGATCAATAATTGAAGATTGCCGG‑‑GGATATCCa                            >  2:4362434/1‑100 (MQ=255)
                                                                            aaTTTGACCGTAAGAAAGCGCTGGCTTATGAGCGTGAAGTGCTGGGCAAATTTGATAAAACTGCGGATCAATAATTGAAGATTGCCGG‑‑GGATATCCAccc                         <  1:4221823/100‑1 (MQ=255)
                                                                                 gACCGTAAGAAAGCGCTGGCTTATGAGCGTGAAGTGCTGGGCAAATTTGATAAAACTGCGGATCAATAATTGAAGATTGCCGG‑‑GGATATCCACCCGGCaa                    >  1:285658/1‑100 (MQ=255)
                                                                                 gACCGTAAGAAAGCGCTGGCTTATGAGCGTGAAGTGCTGGGCAAATTTGATAAAACTGCGGATCAATAATTGAAGATTGCCGG‑‑GGATATCCACCCGGCaa                    >  2:3265833/1‑100 (MQ=255)
                                                                                   ccGTAAGAACGCGCTGACTTATGAGCGTGAATTGCTGGGCAAATGTGATAAAACTGCCGATCAATAATTGAAGATTGCCGG‑‑GGATATCCCCCCGGCAAtg                  <  1:1253896/100‑1 (MQ=255)
                                                                                   ccGTAAGAAAGCGCTGGCTTATGAGCGTGAAGTGCTGGGCAAATTTGATAAAACTGCGGATCAATAATTGAAGATTGCCGG‑‑GGATATCCACCCGGCAAtg                  >  1:3222056/1‑100 (MQ=255)
                                                                                   ccGTAAGAAAGCGCTGGCTTATGAGCGTGAAGTGCTGGGCAAATTTGATAAAACTGCGGATCAATAATTGAAGATTGCCGG‑‑GGATATCCACCCGGCAAtg                  <  2:2163476/100‑1 (MQ=255)
                                                                                   ccGTAAGAAAGCGCTGACTTATGAGCGTGAAGTGCTGGGCAAATTTGATAAAACTGCGGATCAATAATTGAAGATTGCCGGGTGGATATC‑‑CCCGGCAAtg                  >  2:2221011/1‑100 (MQ=38)
                                                                                     gTAAGAAAGCGCTGGCTTATGAGCGTGAAGTGCTGGGCAAATTTGATAAAACTGCGGATCAATAATTGAAGATTGCCGG‑‑GGATATCCACCCGGCAAtgtg                <  1:1554530/100‑1 (MQ=255)
                                                                                     gTAAGAAAGCGCTGGCTTATGAGCGTGAAGTGCTGGGCAAATTTGATAAAACTGCGGATCAATAATTGAAGATTGCCGG‑‑GGATATCCACCCGGCAAtgtg                <  1:1806197/100‑1 (MQ=255)
                                                                                     gTAAGAAAGCGCTGGCTTATGAGCGTGAAGTGCTGGGCAAATTTGATAAAACTGCGGATCAATAATTGAAGATTGCCGG‑‑GGATATCCACCCGGCAAtgtg                <  2:1924697/100‑1 (MQ=255)
                                                                                     gTAAGAAAGCGCTGGCTTATGAGCGTGAAGTGCTGGGCAAATTTGATAAAACTGCGGATCAATAATTGAAGATTGCCGG‑‑GGATATCCACCCGACAAt                   >  1:340133/1‑97 (MQ=255)
                                                                                      tAAGAAAGCGCTGGCTTATGAGCGTGAAGTGCTGGGCAAATTTGATAAAACTGCGGATCAATAATTGAAGATTGCCGG‑‑GGATATCCACCCGGCAAtgtgt               >  1:1455982/1‑100 (MQ=255)
                                                                                       aaGAAAGCGCTGGCTTATGAGCGTGAAGTGCTGGGCAAATTTGATAAAACTGCGGATCAATAATTGAAGATTGCCGG‑‑GGATATCCACCCGGCAAtgtgtg              <  2:1497313/100‑1 (MQ=255)
                                                                                       aaGAAAGCGCTGACTTATGAGCGTGAAGTGCTGGGCAAATTTGATAAAACTGCGGATCAATAATTGAAGATTGCCGG‑‑GGATATCCACCCGGCAAtgtgtg              <  2:2477187/100‑1 (MQ=255)
                                                                                         gagcgcggTGACTTATGAGCGTGACGTGATGGGCAAGTTTTATCAGCCTCCCGCGCAATAATTTAAGAGTTCCGC‑‑GGAGATCCACCCGGCAAtgggt               <  1:1908130/93‑1 (MQ=255)
                                                                                         gAAAGCGCTGGCTTATGAGCGTGAAGTGCTGGGCAAATTTGATAAAACTGCGGATCAATAATTGAAGATTGCCGG‑‑GGATATCCACCCGGCAATGTGTGaa            <  2:3994188/100‑1 (MQ=255)
                                                                                           aaGCGCTGGCTTATGAGCGTGAAGTGCTGGGCAAATTTGATAAAACTGCGGATCAATAATTGAAGATTGCCGG‑‑GGATATCCACCCGGCAATGTGTGAATg          >  2:211745/1‑100 (MQ=255)
                                                                                           aaGCGCTGACTTATGAGCGTGAAGTGCTGGGCAAATTTGATAAAACTGCGGATCAATAATTGAAGATTGCCGG‑‑GGATATCCACCCGGCAATGTGTGAATg          <  2:2803440/100‑1 (MQ=255)
                                                                                             gcgcTGGCTTATGAGCGTGAAGTGCTGGGCAAATTTGATAAAACTGCGGATCAATAATTGAAGATTGCCGG‑‑GGATATCCACCCGGCAATGTGTGAATGcc        <  2:285659/100‑1 (MQ=255)
                                                                                             gcgcTGACTTATGAGCGTGAAGTGCTGGGCAAATTTGATAAAACTGCGGATCAATAATTGAAGATTGCCGG‑‑GGATATCCACCCGGCAATGTGTGAATGcc        >  2:4312973/1‑100 (MQ=255)
                                                                                                   gCTTATGAGCGTGAAGTGCTGGGCAAATTTGATAAAACTGCGGATCAATAATTGAAGATTGCCGG‑‑GGATATCCACCCGGCAATGTGTGAATGCCTGATGc  >  1:2600122/1‑100 (MQ=255)
                                                                                                   |                                                                                                     
CTCGACATGGCGCGTACTGCGCTGAACGTGGTGGGTAATGCGCTGGCGGTGCTGGTGATTGCCAAGTGGGAACACAAATTTGACCGTAAGAAAGCGCTGGCTTATGAGCGTGAAGTGCTGGGCAAATTTGATAAAACTGCGGATCAATAATTGAAGATTGCCGG‑‑GGATATCCACCCGGCAATGTGTGAATGCCTGATGC  >  NC_000913/4295645‑4295843

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A