Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A4 F191 I1 R1 67 105.9 5535243 92.7% 5131170 99.9

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 4,295,744 G→A A422T (GCT→ACT)  gltP → glutamate/aspartate:proton symporter

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,295,7440GA99.1% 391.0 / ‑6.2 113A422T (GCT→ACT) gltPglutamate/aspartate:proton symporter
Reads supporting (aligned to +/- strand):  ref base G (1/0);  new base A (53/59);  total (54/59)
Fisher's exact test for biased strand distribution p-value = 4.78e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.99e-01

Only 100 of 113 total aligned reads displayed.

CGACATGGCGCGTACTGCGCTGAACGTGGTGGGTAATGCGCTGGCGGTGCTGGTGATTGCCAAGTGGGAACACAAATTTGACCGTAAGAAAGCGCTGGCTTATGAGCGTGAAGTGCTGGGCAAATTTGATAAAACTGCGGATCAATAATTGAAGATTGCCGGGGATATCCACCCGGCAATGTGTGAATGCCTGA  >  NC_000913/4295647‑4295840
                                                                                                 |                                                                                                
cGACATGGCGCGTACTGCGCTGAACGTGGTGGGTAATGCGCTGGCGGTGCTGGTGATTGCCAAGTGGGAACACAAATTTGACCGTAAGAAAGCGCTGACt                                                                                                <  1:2607261/100‑1 (MQ=255)
  aCATGGCGCGTACTGCGCTGAACGTGGTGGGTAATGCGCTGGCGGTGCTGGTGATTGCCAAGTGGGAACACAAATTTGACCGTAAGAAAGCGCTGACTTa                                                                                              <  1:402047/100‑1 (MQ=255)
  aCATGGCGCGTACTGCGCTGAACGTGGTGGGTAATGCGCTGGCGGTGCTGGTGATTGCCAAGTGGGAACACAAATTTGACCGTAAGAAAGCGCTGACTTa                                                                                              <  2:2344491/100‑1 (MQ=255)
   cATGGCGCGTACTGCGCTGAACGTGGTGGGTAATGCGCTGGCGGTGCTGGTGATTGCCAAGTGGGAACACAAATTTGACCGTAAGAAAGCGCTGACTTAt                                                                                             >  2:1232993/1‑100 (MQ=255)
      ggCGCGTACTGCGCTGAACGTGGTGGGTAATGCGCTGGCGGTGCTGGTGATTGCCAAGTGGGAACACAAATTTGACCGTAAGAAAGCGCTGACTTATGAg                                                                                          <  2:2745964/100‑1 (MQ=255)
             aCTGCGCTGAACGTGGTGGGTAATGCGCTGGCGGTGCTGGTGATTGCCAAGTGGGAACACAAATTTGACCGTAAGAAAGCGCTGACTTATGAGCGTGAAg                                                                                   >  2:1070102/1‑100 (MQ=255)
             aCTGCGCTGAACGTGGTGGGTAATGCGCTGGCGGTGCTGGTGATTGCCAAGTGGGAACACAAATTTGACCGTAAGAAAGCGCTGACTTATGAGCGTGAAg                                                                                   <  1:1796849/100‑1 (MQ=255)
              cTGCGCTGAACGTGGTGGGTAATGCGCTGGCGGTGCTGGTGATTGCCAAGTGGGAACACAAATTTGACCGTAAGAAAGCGCTGACTTATGAGCGTGAAGt                                                                                  >  2:514244/1‑100 (MQ=255)
              cTGCGCTGAACGTGGTGGGTAATGCGCTGGCGGTGCTGGTGATTGCCAAGTGGGAACACAAATTTGACCGTAAGAAAGCGCTGACTTATGAGCGTGAAGt                                                                                  >  1:2241904/1‑100 (MQ=255)
                gcgcTGAACGTGGTGGGTAATGCGCTGGCGGTGCTGGTGATTGCCAAGTGGGAACACAAATTTGACCGTAAGAAAGCGCTGACTTATGAGCGTGAAGTGc                                                                                >  1:2374452/1‑100 (MQ=255)
                gcgcTGAACGTGGTGGGTAATGCGCTGGCGGTGCTGGTGATTGCCAAGTGGGAACACAAATTTGACCGTAAGAAAGCGCTGACTTATGAGCGTGAAGTGc                                                                                >  1:584816/1‑100 (MQ=255)
                 cgcTGAACGTGGTGGGTAATGCGCTGGCGGTGCTGGTGATTGCCAAGTGGGAACACAAATTTGACCGTAAGAAAGCGCTGACTTATGAGCGTGAAGTGCt                                                                               >  2:2191272/1‑100 (MQ=255)
                 cgcTGAACGTGGTGGGTAATGCGCTGGCGGTGCTGGTGATTGCCAAGTGGGAACACAAATTTGACCGTAAGAAAGCGCTGACTTATGAGCGTGAAGTGCt                                                                               >  1:1733652/1‑100 (MQ=255)
                      aaCGTGGTGGGTAATGCGCTGGCGGTGCTGGTGATTGCCAAGTGGGAACACAAATTTGACCGTAAGAAAGCGCTGACTTATGAGCGTGAAGTGCTGGGCa                                                                          <  1:987450/100‑1 (MQ=255)
                      aaCGTGGTGGGTAATGCGCTGGCGGTGCTGGTGATTGCCAAGTGGGAACACAAATTTGACCGTAAGAAAGCGCTGACTTATGAGCGTGAAGTGCTGGGCa                                                                          <  2:2235976/100‑1 (MQ=255)
                          tggtggGTAATGCGCTGGCGGTGCTGGTGATTGCCAAGTGGGAACACAAATTTGACCGTAAGAAAGCGCTGACTTATGAGCGTGAAGTGCTGGGCAAAtt                                                                      >  2:2285935/1‑100 (MQ=255)
                           ggtggGTAATGCGCTGGCGGTGCTGGTGATTGCCAAGTGGGAACACAAATTTGACCGTAAGAAAGCGCTGACTTATGAGCGTGAAGTGCTGGGCAAAttt                                                                     >  1:1440545/1‑100 (MQ=255)
                            gtggGTAATGCGCTGGCGGTGCTGGTGATTGCCAAGTGGGAACACAAATTTGACCGTAAGAAAGCGCTGACTTATGAGCGTGAAGTGCTGGGCAAATTTg                                                                    <  2:1098745/100‑1 (MQ=255)
                            gtggGTAATGCGCTGGCGGTGCTGGTGATTGCCAAGTGGGAACACAAATTTGACCGTAAGAAAGCGCTGACTTATGAGCGTGAAGTGCTGGGCAAATTTg                                                                    <  1:2191290/100‑1 (MQ=255)
                            gtggGTAATGCGCTGGCGGTGCTGGTGATTGCCAAGTGGGAACACAAATTTGACCGTAAGAAAGCGCTGACTTATGAGCGTGAAGTGCTGGGCAAATTTg                                                                    <  2:948904/100‑1 (MQ=255)
                              gggTAATGCGCTGGCGGTGCTGGTGATTGCCAAGTGGGAACACAAATTTGACCGTAAGAAAGCGCTGACTTATGAGCGTGAAGTGCTGGGCAAATTTGAt                                                                  <  1:2452779/100‑1 (MQ=255)
                              gggTAATGCGCTGGCGGTGCTGGTGATTGCCAAGTGGGAACACAAATTTGACCGTAAGAAAGCGCTGACTTATGAGCGTGAAGTGCTGGGCAAATTTGAt                                                                  <  2:1249067/100‑1 (MQ=255)
                              gggTAATGCGCTGGCGGTGCTGGTGATTGCCAAGTGGGAACACAAATTTGACCGTAAGAAAGCGCTGACTTATGAGCGTGAAGTGCTGGGCAAATTTGAt                                                                  <  2:674948/100‑1 (MQ=255)
                               ggTAATGCGCTGGCGGTGCTGGTGATTGCCAAGTGGGAACACAAATTTGACCGTAAGAAAGCGCTGACTTATGAGCGTGAAGTGCTGGGCAAATTTGATa                                                                 <  1:2086186/100‑1 (MQ=255)
                               ggTAATGCGCTGGCGGTGCTGGTGATTGCCAAGTGGGAACACAAATTTGACCGTAAGAAAGCGCTGACTTATGAGCGTGAAGTGCTGGGCAAATTTGATa                                                                 >  1:1705229/1‑100 (MQ=255)
                                 tAATGCGCTGGCGGTGCTGGTGATTGCCAAGTGGGAACACAAATTTGACCGTAAGAAAGCGCTGACTTATGAGCGTGAAGTGCTGGGCAAATTTGATaaa                                                               <  1:877841/100‑1 (MQ=255)
                                   aTGCGCTGGCGGTGCTGGTGATTGCCAAGTGGGAACACAAATTTGACCGTAAGAAAGCGCTGACTTATGAGCGTGAAGTGCTGGGCAAATTTGATAAAAc                                                             <  2:1840340/100‑1 (MQ=255)
                                   aTGCGCTGGCGGTGCTGGTGATTGCCAAGTGGGAACACAAATTTGACCGTAAGAAAGCGCTGACTTATGAGCGTGAAGTGCTGGGCAAATTTGATAAAAc                                                             >  1:1775240/1‑100 (MQ=255)
                                   aTGCGCTGGCGGTGCTGGTGATTGCCAAGTGGGAACACAAATTTGACCGTAAGAAAGCGCTGACTTATGAGCGTGAAGTGCTGGGCAAATTTGATAAAAc                                                             >  1:609550/1‑100 (MQ=255)
                                     gcgcTGGCGGTGCTGGTGATTGCCAAGTGGGAACACAAATTTGACCGTAAGAAAGCGCTGACTTATGAGCGTGAAGTGCTGGGCAAATTTGATAAAACTg                                                           >  2:860677/1‑100 (MQ=255)
                                      cgcTGGCGGTGCTGGTGATTGCCAAGTGGGAACACAAATTTGACCGTAAGAAAGCGCTGACTTATGAGCGTGAAGTGCTGGGCAAATTTGATAAAACTGc                                                          >  2:2225247/1‑100 (MQ=255)
                                       gcTGGCGGTGCTGGTGATTGCCAAGTGGGAACACAAATTTGACCGTAAGAAAGCGCTGACTTATGAGCGTGAAGTGCTGGGCAAATTTGATAAAACTGCg                                                         <  2:1020351/100‑1 (MQ=255)
                                        cTGGCGGTGCTGGTGATTGCCAAGTGGGAACACAAATTTGACCGTAAGAAAGCGCTGACTTATGAGCGTGAAGTGCTGGGCAAATTTGATAAAACTGCgg                                                        >  2:1119758/1‑100 (MQ=255)
                                        cTGGCGGTGCTGGTGATTGCCAAGTGGGAACACAAATTTGACCGTAAGAAAGCGCTGACTTATGAGCGTGAAGTGCTGGGCAAATTTGATAAAACTGCgg                                                        >  2:1056586/1‑100 (MQ=255)
                                          ggCGGTGCTGGTGATTGCCAAGTGGGAACACAAATTTGACCGTAAGAAAGCGCTGACTTATGAGCGTGAAGTGCTGGGCAAATTTGATAAAACTGCGGAt                                                      >  1:208534/1‑100 (MQ=255)
                                          ggCGGTGCTGGTGATTGCCAAGTGGGAACACAAATTTGACCGTAAGAAAGCGCTGACTTATGAGCGTGAAGTGCTGGGCAAATTTGATAAAACTGCGGAt                                                      <  2:2635989/100‑1 (MQ=255)
                                           gCGGTGCTGGTGATTGCCAAGTGGGAACACAAATTTGACCGTAAGAAAGCGCTGACTTATGAGCGTGAAGTGCTGGGCAAATTTGATAAAACTGCGGATc                                                     <  2:1246316/100‑1 (MQ=255)
                                           gCGGTGCTGGTGATTGCCAAGTGGGAACACAAATTTGACCGTAAGAAAGCGCTGACTTATGAGCGTGAAGTGCTGGGCAAATTTGATAAAACTGCGGATc                                                     <  2:2241886/100‑1 (MQ=255)
                                           gCGGTGCTGGTGATTGCCAAGTGGGAACACAAATTTGACCGTAAGAAAGCGCTGACTTATGAGCGTGAAGTGCTGGGCAAATTTGATAAAACTGCGGATc                                                     <  1:2025455/100‑1 (MQ=255)
                                            cGGTGCTGGTGATTGCCAAGTGGGAACACAAATTTGACCGTAAGAAAGCGCTGACTTATGAGCGTGAAGTGCTGGGCAAATTTGATAAAACTGCGGATc                                                     <  2:1031245/99‑1 (MQ=255)
                                            cGGTGCTGGTGATTGCCAAGTGGGAACACAAATTTGACCGTAAGAAAGCGCTGACTTATGAGCGTGAAGTGCTGGACAAATTTGATAAAACTGCGGa                                                       >  1:1299139/1‑97 (MQ=255)
                                             ggTGCTGGTGATTGCCAAGTGGGAACACAAATTTGACCGTAAGAAAGCGCTGACTTATGAGCGTGAAGTGCTGGGCAAATTTGATAAAACTGCGGATCaa                                                   <  1:1126471/100‑1 (MQ=255)
                                              gTGCTGGTGATTGCCAAGTGGGAACACAAATTTGACCGTAAGAAAGCGCTGACTTATGAGCGTGAAGTGCTGGGCAAATTTGATAAAACTGCGGATCaat                                                  <  1:2736571/100‑1 (MQ=255)
                                                gCTGGTGATTGCCAAGTGGGAACACAAATTTGACCGTAAGAAAGCGCTGACTTATGAGCGTGAAGTGCTGGGCAAATTTGATAAAACTGCGGATCaataa                                                <  1:717124/100‑1 (MQ=255)
                                                 cTGGTGATTGCCAAGTGGGAACACAAATTTGACCGTAAGAAAGCGCTGACTTATGAGCGTGAAGTGCTGGGCAAATTTGATAAAACTGCGGATCaataat                                               >  1:1059810/1‑100 (MQ=255)
                                                  tGGTGATTGCCAAGTGGGAACACAAATTTGACCGTAAGAAAGCGCTGACTTATGAGCGTGAAGTGCTGGGCAAATTTGATAAAACTGCGGATCAATAAtt                                              >  2:714424/1‑100 (MQ=255)
                                                   ggTGATTGCCAAGTGGGAACACAAATTTGACCGTAAGAAAGCGCTGACTTATGAGCGTTAAGTGCTGGGCAAATTTGATAAAACTGCGGACCAATAATTg                                             <  2:2151286/100‑1 (MQ=255)
                                                   ggTGATTGCCAAGTGGGAACACAAATTTGACCGTAAGAAAGCGCTGACTTATGAGCGTGAAGTGCTGGGCAAATTTGATAAAACTGCGGATCAATAATTg                                             <  2:2151287/100‑1 (MQ=255)
                                                   ggTGATTGCCAAGTGGGAACACAAATTTGACCGTAAGAAAGCGCTGACTTATGAGCGTGAAGTGCTGGGCAAATTTGATAAAACTGCGGATCAATAATTg                                             >  1:716294/1‑100 (MQ=255)
                                                    gatcttGCCAAGTGGGAACACAAATTTGACCGTAAGACAGCGCTGACCTATGAGCGTGAAGTGCTGGGCACATTTGATAAAACTGCGGATCAATAATTGa                                            <  1:1508001/96‑1 (MQ=255)
                                                     tGATTGCCAAGTGGGAACACAAATTTGACCGTAAGAAAGCGCTGACTTATGAGCGTGAAGTGCTGGGCAAATTTGATAAAACTGCGGATCAATAATTGaa                                           >  2:327402/1‑100 (MQ=255)
                                                     tGATTGCCAAGTGGGAACACAAATTTGACCGTAAGAAAGCGCTGACTTATGAGCGTGAAGTGCTGGGCAAATTTGATAAAACTGCGGATCAATAATTGaa                                           >  2:15166/1‑100 (MQ=255)
                                                     tGATTGCCAAGTGGGAACACAAATTTGACCGTAAGAAAGCGCTGACTTATGAGCGTGAAGTGCTGGGCAAATTTGATAAAACTGCGGATCAATAATTGaa                                           <  2:1316111/100‑1 (MQ=255)
                                                      gATTGCCAAGTGGGAACACAAATTTGACCGTAAGAAAGCGCTGACTTATGAGCGTGAAGTGCTGGGCAAATTTGATAAAACTGCGGATCAATAATTGAAg                                          >  2:29482/1‑100 (MQ=255)
                                                      gATTGCCAAGTGGGAACACAAATTTGACCGTAAGAAAGCGCTGACTTATGAGCGTGAAGTGCTGGGCAAATTTGATAAAACTGCGGATCAATAATTGAAg                                          <  1:2512727/100‑1 (MQ=255)
                                                      gATTGCCAAGTGGGAACACAAATTTGACCGTAAGAAAGCGCTGACTTATGAGCGTGAAGTGCTGGGCAAATTTGATAAAACTGCGGATCAATAATTGAAg                                          <  2:83484/100‑1 (MQ=255)
                                                        ttGCCAAGTGGGAACACAAATTTGACCGTAAGAAAGCGCTGACTTATGAGCGTGAAGTGCTGGGCAAATTTGATAAAACTGCGAATCAATAATTGAAGAt                                        >  2:1613063/1‑100 (MQ=255)
                                                         tGCCAAGTGGGAACACAAATTTGACCGTAAGAAAGCGCTGAGTTATGAGCGTGAAGTGCTGTGCAAATTTGATAAAACTGCGGATCAATAATTGAAGAtc                                       >  2:1507989/1‑99 (MQ=255)
                                                          gCCAAGTGGGAACACAAATTTGACCGTAAGAAAGCGCTGACTTATGAGCGTGAAGTGCTGGGCAAATTTGATAAAACTGCGGATCAATAATTGAAGATTg                                      >  1:815208/1‑100 (MQ=255)
                                                           ccAAGTGGGAACACAAATTTGACCGTAAGAAAGCGCTGACTTATGAGCGTGAAGTGCTGGGTAAATTTGATAAAACTGCGGATCAATAATTGAAGATTGc                                     <  2:1707624/100‑1 (MQ=255)
                                                           ccAAGTGGGAACACAAATTTGACCGTAAGAAAGCGCTGACTTATGAGCGTGAAGTGCTGGGCAAATTTGATAAAACTGCGGATCAATAATTGAAGATTGc                                     <  1:2081076/100‑1 (MQ=255)
                                                           ccAAGTGGGAACACAAATTTGACCGTAAGAAAGCGCTGACTTATGAGCGTGAAGTGCTGGGCAAATTTGATAAAACTGCGGATCAATAATTGAAGATTGc                                     >  1:2004350/1‑100 (MQ=255)
                                                            cAAGTGGGAACACAAATTTGACCGTAAGAAAGCGCTGACTTATGAGCGTGAAGTGCTGGGCAAATTTGATAAAACTGCGGATCAATAATTGAAGATTGcc                                    >  2:2578333/1‑100 (MQ=255)
                                                                tGGGAACACAAATTTGACCGTAAGAAAGCGCTGACTTATGAGCGTGAAGTGCTGGGCAAATTTGATAAAACTGCGGATCAATAATTGAAGATTGCCgggn                                <  2:563848/100‑2 (MQ=255)
                                                                 gggAACACAAATTTGACCGTAAGAAAGCGCTGACTTATGAGCGTGAAGTGCTGGGCAAATTTGATAAAACTGCGGATCAATAATTGAAGATTGCCGGGGa                               <  1:1121289/100‑1 (MQ=255)
                                                                 gggAACACAAATTTGACCGTAAGAAAGCGCTGACTTATGAGCGTGAAGTGCTGGGCAAATTTGATAAAACTGCGGATCAATAATTGAAGATTGCCGGGGa                               >  1:1275592/1‑100 (MQ=255)
                                                                 gggAACACAAATTTGACCGTAAGAAAGCGCTGACTTATGAGCGTGAAGTGCTGGGCAAATTTGATAAAACTGCGGATCAATAATTGAAGATTGCCGGGGa                               <  2:883415/100‑1 (MQ=255)
                                                                 gggAACACAAATTTGACCGTAAGAAAGCGCTGACTTATGAGCGTGAAGTGCTGGGCAAATTTGATAAAACTGCGGATCAATAATTGAAGATTGCCGGGGa                               >  1:2033920/1‑100 (MQ=255)
                                                                  ggAACACAAATTTGACCGTAAGAAAGCGCTGACTTATGAGCGTGAAGTGCTGGGCAAATTTGATAAAACTGCGGATCAATAATTGAAGATTGCCGGGGat                              >  2:901978/1‑100 (MQ=255)
                                                                   gAACACAAATTTGACCGTAAGAAAGCGCTGACTTATGAGCGTGAAGTGCTGGGCAAATTTGATAAAACTGCGGATCAATAATTGAAGATTGCCGGGGata                             >  2:300002/1‑100 (MQ=255)
                                                                    aaCACAAATTTGACCGTAAGAAAGCGCTGACTTATGAGCGTGAAGTGCTGGGCAAATTTGATAAAACTGCGGATCAATAATTGAAGATTGCCGGGGatat                            >  2:619760/1‑100 (MQ=255)
                                                                    aaCACAAATTTGACCGTAAGAAAGCGCTGACTTATGAGCGTGAAGTGCTGGGCAAATTTGATAAAACTGCGGATCAATAATTGAAGATTGCCGGGGatat                            <  2:2334626/100‑1 (MQ=255)
                                                                     acacAAATTTGACCGTAAGAAAGCGCTGACTTATGAGCGTGAAGTGCTGGGCAAATTTGATAAAACTGCGGATCAATAATTGAAGATTGCCGGGGATATc                           <  2:1170464/100‑1 (MQ=255)
                                                                      cccaAATTTGACATTAAGAAAGTGCTGACTTATGAGCGTGAAGTGCTGGGCAAATTTGATAAAACTGCGGATCAATAATTGAAGATTGCCGGGGATATcc                          >  2:925968/3‑100 (MQ=255)
                                                                      cacaAATTTGACCGTAAGAAAGCGCTGACTTATGAGCGTGAAGTGCTGGGCAAATTTGATAAAACTGCGGATCAATAATTGAAGATTGCCGGGGATATcc                          >  1:1231287/1‑100 (MQ=255)
                                                                        caAATTTGACCGTAAGAAAGCGCTGACTTATGAGCGTGAAGTGCTGGGCAAATTTGATAAAACTGCGGATCAATAATTGAAGATTGCCGGGGATATCCAc                        >  1:998193/1‑100 (MQ=255)
                                                                        caAATTTGACCGTAAGAAAGCGCTGACTTATGAGCGTGAAGTGCTGGGCAAATTTGATAAAACTGCGGATCAATAATTGAAGATTGCCGGGGATATCCAc                        >  2:2553222/1‑100 (MQ=255)
                                                                         aaaTTTGACCGTAAGAAAGCGCTGACTTATGAGCGTGAAGTGCTGGGCAAATTTGATAAAACTGCGGATCAATAATTGAAGATTGCCGGGGATATCCAcc                       >  2:2073544/1‑100 (MQ=255)
                                                                          gatctGACCGTAAGAAAGCGCTGACTTATGAGCGTGAAGTGCTGGGCAAATTTGATAAAACTGCGGATCAATAATTGAAGATTGCCGGGGATATCCAccc                      <  1:976274/96‑1 (MQ=255)
                                                                          aaTTTGACCGTAAGAAAGCGCTGACTTATGAGCGTGAAGTGCTGGGCAAATTTGATAAAACTGCGGATCAATAATTGAAGATTGCCGGGGATATCCAccc                      <  2:907936/100‑1 (MQ=255)
                                                                          aaTTTGACCGTAAGAAAGCGCTGACTTATGAGCGTGAAGTGCTGGGCAAATTTGATAAAACTGCGGATCAATAATTGAAGATTGCCGGGGATATCCAccc                      <  2:208531/100‑1 (MQ=255)
                                                                           aTTTGACCGTAAGAAAGCGCTGACTTATGAGCGTGAAGTGCTGGGCAAATTTGATAAAACTGCGGATCAATAATTGAAGATTGCCGGGGATATCCACCCg                     <  2:1775226/100‑1 (MQ=255)
                                                                                  cGTAAGAAAGCGCTGACTTATGAGCGTGAAGTGCTGGGCAAATTTGATAAAACTGCGGATCAATAATTGAAGATTGCCGGGGATATCCACCCGGCAAtgt              <  1:353966/100‑1 (MQ=255)
                                                                                  cGTAAGAAAGCGCTGACTTATGAGCGTGAAGTGCTGGGCAAATTTGATAAAACTGCGGATCAATAATTGAAGATTGCCGGGGATATCCACCCGGCAAtgt              >  1:1548557/1‑100 (MQ=255)
                                                                                   ggaaGAAATCGCTGACTTATGAGCGTGAAGTGCTGGGCAAATTTGATAAAACTGCGGATCAATAATTGAAGATTGCCGGGGATATCCACCCGGCAAtgtg             <  2:2374433/98‑1 (MQ=255)
                                                                                   gTAAGAAAGCGCTGACTTATGAGCGTGAAGTGCTGGGCAAATTTGATAAAACTGCGGATCAATAATTGAAGATTGCCGGGGATATCCACCCGGCAAtgtg             <  2:2126575/100‑1 (MQ=255)
                                                                                    tAAGAAAGCGCTGACTTATGAGCGTGAAGTGCTGGGCAAATTTGATAAAACTGCGGATCAATAATTGAAGATTGCCGGGGATATCCACCCGGCAAtgtgt            >  2:2429079/1‑100 (MQ=255)
                                                                                    tAAGAAAGCGCTGACTTATGAGCGTGAAGTGCTGGGCAAATTTGATAAAACTGCGGATCAATAATTGAAGATTGCCGGGGATATCCACCCGGCAAtgtgt            <  2:1275585/100‑1 (MQ=255)
                                                                                      aGAAAGCGCTGGCTTATGAGCGTGAAGTGCTGGGCAAATTTGATAAAACTGCGGATCAATAATTGAAGATTGCCGGGGATATCCACCCGGCAATGTGTGa          >  2:2227753/1‑100 (MQ=255)
                                                                                      aGAAAGCGCTGACTTATGAGCGTGAAGTGCTGGGCAAATTTGATAAAACTGCGGATCAATAATTGAAGATTGCCGGGGATATCCACCCGGCAATGTGTGa          >  2:1630392/1‑100 (MQ=255)
                                                                                      aGAAAGCGCTGACTTATGAGCGTGAAGTGCTGGGCAAATTTGATAAAACTGCGGATCAATAATTGAAGATTGCCGGGGATATCCACCCGGCAATGTGTGa          <  2:1748598/100‑1 (MQ=255)
                                                                                      aGAAAGCGCTGACTTATGAGCGTGAAGTGCTGGGCAAAGGTGATAAAACTGCGGATCAATAATTGAAGATTGCCGGGGATATCCACCCGGCAATGTGTGa          <  2:2428253/100‑1 (MQ=255)
                                                                                       gAAAGCGCTGACTTATGAGCGTGAAGTGCTGGGCAAATTTGATAAAACTGCGGATCAATAATTGAAGATTGCCGGGGATATCCACCCGGCAATGTGTGaa         >  1:1911038/1‑100 (MQ=255)
                                                                                        aaaGCGCTGACTTATGAGCGTGAAGTGCTGGGCAAATTTGATAAAACTGCGGATCAATAATTGAAGATTGCCGGGGATATCCACCCGGCAATGTGTGAAt        <  1:1613076/100‑1 (MQ=255)
                                                                                        aaaGCGCTGACTTATGAGCGTGAAGTGCTGGGCAAATTTGATAAAACTGCGGATCAATAATTGAAGATTGCCGGGGATATCCACCCGGCAATGTGTGAAt        <  1:860683/100‑1 (MQ=255)
                                                                                           gcgcTGACTTATGAGCGTGAAGTGCTGGGCAAATTTGATAAAACTGCGGATCAATAATTGAAGATTGCCGGGGATATCCACCCGGCAATGTGTGAATGcc     <  1:1133323/100‑1 (MQ=255)
                                                                                           gcgcTGACTTATGAGCGTGAAGTGCTGGGCAAATTTGATAAAACTGCGGATCAATAATTGAAGATTGCCGGGGATATCCACCCGGCAATGTGTGAATGcc     <  1:2578352/100‑1 (MQ=255)
                                                                                           gcgcTGACTTATGAGCGTGAAGTGCTGGGCAAATTTGATAAAACTGCGGATCAATAATTGAAGATTGCCGGGGATATCCACCCGGCAATGTGTGAATGcc     >  2:1967649/1‑100 (MQ=255)
                                                                                           gcgcTGACTTATGAGCGTGAAGTGCTGGGCAAATTTGATAAAACTGCGGATCAATAATTGAAGATTGCCGGGGATATCCACCCGACAATGTGTGAATGcc     >  2:1596221/1‑100 (MQ=255)
                                                                                              cTGACTTATGAGCGTGAAGTGCTGGGCAAATTTGATAAAACTGCGGATCAATAATTGAAGATTGCCGGGGATATCCACCCGGCAATGTGTGAATGCCTGa  >  2:388771/1‑100 (MQ=255)
                                                                                                 |                                                                                                
CGACATGGCGCGTACTGCGCTGAACGTGGTGGGTAATGCGCTGGCGGTGCTGGTGATTGCCAAGTGGGAACACAAATTTGACCGTAAGAAAGCGCTGGCTTATGAGCGTGAAGTGCTGGGCAAATTTGATAAAACTGCGGATCAATAATTGAAGATTGCCGGGGATATCCACCCGGCAATGTGTGAATGCCTGA  >  NC_000913/4295647‑4295840

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A