Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A3 F228 I0 R1 182 117.2 5818924 93.6% 5446512 99.8

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 3,951,598:1 +T 100% pseudogene (57/663 nt) ilvG → pseudogene, acetolactate synthase 2 large subunit, valine‑insensitive; acetolactate synthase II, large subunit, cryptic, interrupted

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,951,5971.T92.3% 328.1 / 19.8 104pseudogene (56/663 nt)ilvGpseudogene, acetolactate synthase 2 large subunit, valine‑insensitive; acetolactate synthase II, large subunit, cryptic, interrupted
Reads supporting (aligned to +/- strand):  ref base . (3/5);  new base T (37/59);  total (40/64)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.03e-01

Only 100 of 109 total aligned reads displayed.

TTTAAATGCTCTGTTACCAGCATTACAGCAGCCGTTAAATCAATGACTGGCAGCAACACTGCGCGCAGCTGCGTGATGAACATTCCTGGCGTTACGACCA‑TCCCGGTGACGCTATCTACGCGCCGTTGTTGTTAA‑AACAACTGTCGGATCGTAAACCTGCGGATTGCGTCGTGACCACAGATGTGGGGCAGCAC  >  NC_000913/3951498‑3951691
                                                                                                    |                                                                                               
tttAAATGCTCTGTTACCAGCATTACAGCAGCCGTTAAATCAATGACTGGCAGCAACACTGCGCGCAGCTGCGTGATGAACATTCCTGGCGTTACGACCa                                                                                                  >  2:1407368/1‑100 (MQ=255)
tttAAATGCTCTGTTACCAGCATTACAGCAGCCGTTAAATCAATGACTGGCAGCAACACTGCGCGCAGCTGCGTGATGAACATTCCTGGCGTTACGACCa                                                                                                  <  1:1121050/100‑1 (MQ=255)
tttAAATGCTCTGTTACCAGCATTACAGCAGCCGTTAAATCAAGGACTGGCAGCAACACTGCGCGCAGCTGCGTGATGAACATTCCTGGCGTTACGACCa                                                                                                  <  1:1861399/100‑1 (MQ=255)
  tAAATGCTCTGTTACCAGCATTACCGCAGCCGTTAAATCAATGACTGGCAGCAACACTGCGCGCAGCTGCGTGATGAACATTCCTGGCGTTACGACCA‑tt                                                                                               >  1:2651905/1‑99 (MQ=255)
     aTGCTCTGTTACCAGCATTACAGCAGCCGTTAAATCAATGACTGGCAGCAACACTGCGCGCAGCTGCGTGATGAACATTCCTGGCGTTACGACCA‑Ttccc                                                                                            <  2:1289919/100‑2 (MQ=255)
       gCTCTGTTACCAGCATTACAGCAGCCGTTAAATCAATGACTGGCAGCAACACTGCGCGCAGCTGCGTGATGAACATTCCTGGCGTTACGACCATTCCCgg                                                                                           <  2:21276/100‑1 (MQ=255)
       gCTCTGTTACCAGCATTACAGCAGCCGTTAAATCAATGACTGGCAGCAACACTGCGCGCAGCTGCGTGATGAACATTCCTGGCGTTACGACCA‑TCCCGGt                                                                                          <  1:1556104/100‑1 (MQ=255)
        ctctGTTGCCAGCATTACGGCAGCCGTGAAATCAATGACTGGCAGCAACACTGCGCGCAGCTGCGTGATGAACATCACTGGCGTTACGACCA‑ttgccg                                                                                           >  1:2318484/1‑93 (MQ=255)
        ctctGTTACCAGCATTACAGCAGCCGTTAAATCAATGACTGGCAGCAACACTGCGCGCAGCTGCGTGATGAACATTCCTGGCGTTACGACCATTCCCGGt                                                                                          <  2:1314401/100‑1 (MQ=255)
        ctctGTTACCAGCATTACAGCAGCCGTTAAATCAATGACTGGCAGCAACACTGCGCGCAGCTGCGTGATGAACATTCCTGGCGTTACGACCATTCCCGGt                                                                                          <  2:2573955/100‑1 (MQ=255)
        ctctGTTACCAGCATTACAGCAGCCGTTAAATCAATGACTGGCAGCAACACTGCGCGCAGCTGCGTGATGAACATTCCTGGCGTTACGACCATTCCCGGt                                                                                          >  1:2318428/1‑100 (MQ=255)
         tctGTTACCAGCATTACAGCAGCCGTTAAATCAATGACTGGCAGCAACACTGCGCGCAGCTGCGTGATGAACATTCCTGGCGTTACGACCA‑TCCCGGTGa                                                                                        <  1:2273860/100‑1 (MQ=255)
           tGTTACCAGCATTACAGCAGCCGTTAAATCAATGACTGGCAGCAACACTGCGCGCAGCTGCGTGATGAACATTCCTGGCGTTACGACCATTCCCGGTGAc                                                                                       <  1:1708353/100‑1 (MQ=255)
             ttACCAGCATTACAGCAGCCGTTAAATCAATGACTGGCAGCAACACTGCGCGCAGCTGCGTGATGAACATTCCTGGCGTTACGACCATTCCCGGTGACGc                                                                                     <  2:1004820/100‑1 (MQ=255)
              tACCAGCATTACAGCAGCCGTTAAATCAATGACTGGCAGCAACACTGCGCGCAGCTGCGTGATGAACATTCCTGGCGTTACGACCA‑TCCCGGTGACGCTa                                                                                   >  1:2155821/1‑100 (MQ=255)
               aCCAGCATTACAGCAGCCGTTAAATCAATGACTGGCAGCAACACTGCGCGCAGCTGCGTGATGAACATTCCTGGCGTTACGACCATTCCCGGTGACGCTa                                                                                   >  2:2353090/1‑100 (MQ=255)
                ccAGCATTACAGCAGCCGTTAAATCAATGACTGGCAGCAACACTGCGCGCAGCTGCGTGATGAACATTCCTGGCGTTACGACCATTCCCGGTGACGCTAt                                                                                  >  2:638295/1‑100 (MQ=255)
                 cAGCATTACAGCAGCCGTTAAATCAATGACTGGCAGCAACACTGCGCGCAGCTGCGTGATGAACATTCCTGGCGTTACGACCATTCCCGGTGACGCTATc                                                                                 <  1:2482615/100‑1 (MQ=255)
                 cAGCATTACAGCAGCCGTTAAATCAATGACTGGCAGCAACACTGCGCGCAGCTGCGTGATGAACATTCCTGGCGTTACGACCATTCCCGGTGACGCTATc                                                                                 <  2:851316/100‑1 (MQ=255)
                 cAGCATTACAGCAGCCGTTAAATCAATGACTGGCAGCAACACTGCGCGCAGCTGCGTGATGAACATTCCTGGCGTTACGACCATTCCCGGTGACGCTATc                                                                                 <  2:984339/100‑1 (MQ=255)
                  aGCATTACAGCAGCCGTTAAATCAATGACTGGCAGCAACACTGCGCGCAGCTGCGTGATGAACATTCCTGGCGTTACGACCATTCCCGGTGACGCTATCt                                                                                >  2:2411741/1‑100 (MQ=255)
                   gCATTACAGCAGCCGTTAAATCAATGACTGGCAGCAACACTGCGCGCAGCTGCGTGATGAACATTCCTGGCGTTACGACCATTCCCGGTGACGCTATCTa                                                                               <  1:179695/100‑1 (MQ=255)
                      ttACAGCAGCCGTTAAATCAATGACTGGCAGCAACACTGCGCGCAGCTGCGTGATGAACATTCCTGGCGTTACGACCATTCCCGGTGACGCTATCTAcg                                                                             >  1:463716/1‑99 (MQ=255)
                       tACAGCAGCCGTTAAATCAATGACTGGCAGCAACACTGCGCGCAGCTGCGTGATGAAGATACCTGGCATTACGACCATTCCCGGTGACGCTATCTAcgcg                                                                           >  1:2043519/1‑100 (MQ=255)
                         cagcagCCGTTAAATCAAGGACTGGCAGCAACACTGCGCGCAGCTGCGTGATGAACATTCCTGGCGTTACGACCATTCCCGGTGACGCTATCTACGCGcc                                                                         <  2:2726168/100‑1 (MQ=255)
                           gcagcCGTTAAATCAATGACTGGCAGCAACACTGCGCGCAGCTGCGTGATGAACATTCCTGGCGTTACGACCATTCCCGGTGACGCTATCTACGCGCCgt                                                                       <  1:2088461/100‑1 (MQ=255)
                             agcCGTTAAATCAATGACTGGCAGCAACACTGCGCGCAGCTGCGTGATGAACATTCCTGGCGTTACGACCATTCCCGGTGACGCTATCTACGCGCCgttg                                                                     >  1:1303456/1‑100 (MQ=255)
                              gcCGTTAAATCAATGACTGGCAGCAACACTGCGCGCAGCTGCGTGATGAACATTCCTGGCGTTACGACCATTCCCGGTGACGCTATCTACGCGCCgttgt                                                                    >  2:1325137/1‑100 (MQ=255)
                               ccGTTAAATCAATGACTGGCAGCAACACTGCGCGCAGCTGCGTGATGAACATTCCTGGCGTTACGACCATTCCCGGTGACGCTATCTACGCGTCgttgtt                                                                   <  2:1527201/100‑1 (MQ=255)
                               ccGTTAAATCAATGACTGGCAGCAACACTGCGCGCAGCTGCGTGATGAACATTCCTGGCGTTACGACCATTCCCGGTGACGCTATCTACGCGCCgttgtt                                                                   <  2:1859916/100‑1 (MQ=255)
                               ccGTTAAATCAATGACTGGCAGCAACACTGCGCGCAGCTGCGTGATGAACATTCCTGGCGTTACGACCATTCCCGGTGACGCTATCTACGCGCCgttgtt                                                                   <  2:497183/100‑1 (MQ=255)
                               ccGTTAAATCAATGACTGGCAGCAACACTGCGCGCAGCTGCGTGATGAACATTCCTGGCGTTACGACCATTCCCGGTGACGCTATCTACGCGCCgttgt                                                                    >  1:967559/1‑99 (MQ=255)
                                cGTTAAATCAATGACTGGCAGCAACACTGCGCGCAGCTGCGTGATGAACATTCCTGGCGTTACGACCATTCCCGGTGACGCTATCTACGCGCCgttgttg                                                                  >  1:988056/1‑100 (MQ=255)
                                cGTTAAATCAATGACTGGCAGCAACACTGCGCGCAGCTGCGTGATGAACATTCCTGGCGTTACGACCA‑TCCCGGTGACGCTATCTACGCGCCgttgttgt                                                                 >  1:2312177/1‑100 (MQ=255)
                                 gTTAAATCAATGACTGGCAGCAACACTGCGCGGAGCTGCGTGATGAACATTCCTGGCGTTACGACCATTCCCGGTGACGCTATCTACGCGCCgttgtt                                                                   >  1:2723803/1‑98 (MQ=255)
                                 gTTAAATCAATGACTGGCAGCAACACTGCGCGCAGCTGCGTGATGAACATTCCTGGCGTTACGACCATTCCCGGTGACGCTATCTACGCGCCgttgttgt                                                                 <  2:1071537/100‑1 (MQ=255)
                                 gTTAAATCAATGACTGGCAGCAACACTGCGCGCAGCTGCGTGATGAACATTCCTGGCGTTACGACCATTCCCGGTGACGCTATCTACGCGCCgttgttgt                                                                 <  1:1013495/100‑1 (MQ=255)
                                  ttAAATCAATGACTGGCAGCAACACTGCGCGCAGCTGCGTGATGAACATTCCTGGCGTTACGACCATTCCCGGTGACGCTATCTACGCGCCgttgttgtt                                                                <  2:2859412/100‑1 (MQ=255)
                                   tAAATCAATGACTGGCAGCAACACTGCGCGCAGCTGCGTGATGAACATTCCTGGCGTTACGACCATTCCCGGTGACGCTATCTACGCGCCGTTGTTGTTa                                                               <  2:1313312/100‑1 (MQ=255)
                                    aaaTCAATGACTGGCAGCAACACTGCGCGCAGCTGCGTGATGAACATTCCTGGCGTTACGACCATTCCCGGTGACGCTATCTACGCGCCGTTGTTGTTaa                                                              >  2:2069008/1‑100 (MQ=255)
                                     aaTCAATGACTGGCAGCAACACTGCGCGCAGCTGCGTGATGAACATTCCTGGCGTTACGACCATTCCCGGTGACGCTATCTACGCGCCGTTGTTGTTaa‑a                                                            <  1:2137130/100‑1 (MQ=255)
                                     aaTCAATGACTGGCAGCAACACTGCGCGCAGCTGCGTGATGAACATTCCTGGCGTTACGACCATTCCCGGTGACGCTATCTACGCGCCGTTGTTGTTaa‑a                                                            <  2:974873/100‑1 (MQ=255)
                                      aTCAATGACTGGCAGCAACACTGCGCGCAGCTGCGTGATGAACATTCCTGGCGTTACGACCATTCCCGGTGACGCTATCTACGCGCCGTTGTTGTTaa‑aa                                                           >  1:409843/1‑100 (MQ=255)
                                      aTCAATGACTGGCAGCAACACTGCGCGCAGCTGCGTGATGAACATTCCTGGCGTTACGACCATTCCCGGTGACGCTATCTACGCGCCGTTGTTGTTaa‑aa                                                           >  1:732461/1‑100 (MQ=255)
                                      aTCAATGACTGACAGCAACACTGCGCGCAGCTGCGTGATGAACATTCCTGGCGTTACGACCATTCCCGGTGACGCTATCTACGCGCCGTTGTTGTTaa‑aa                                                           <  1:1578626/100‑1 (MQ=255)
                                        cAATGACTGGCAGCAACACTGCGCGCAGCTGCGTGATGAACATTCCTGGCGTTACGACCATTCCCGGTGACGCTATCTACGCGCCGTTGTTGTTac‑aaca                                                         >  1:2834978/1‑100 (MQ=255)
                                        cAATGACTGGCAGCAACACTGCGCGCAGCTGCGTGATGAACATTCCTGGCGTTACGACCATTCCCGGTGACGCTATCTACGCGCCGTTGTTGTTAA‑aaca                                                         <  2:182254/100‑1 (MQ=255)
                                        cAATGACTGGCAGCAACACTGCGCGCAGCTGCGTGATGAACATTCCTGGCGTTACGACCATTCCCGGTGACGCTATCTACGCGCCGTTGTTGTTAA‑aaca                                                         >  1:2025661/1‑100 (MQ=255)
                                         aaTGACTGGCAGCAACACTGCGCGCAGCTGCGTGATGAACATTCCTGGCGTTACGACCATTCCCGGTGACGCTATCTACGCGCCGTTGTTGTTAA‑aacaa                                                        >  1:1495371/1‑100 (MQ=255)
                                           tGACTGGCAGCAACACTGCGCGCAGCTGCGTGATGAACATTCCTGGCGTTACGACCATTCCCGGTGACGCTATCTACGCGCCGTTGTTGTTAA‑AACAACt                                                      <  2:2043526/100‑1 (MQ=255)
                                             aCTGGCAGCAACACTGCGCGCAGCTGCGTGATGAACATTCCTGGCGTTACGACCATTCCCGGTGACGCTATCTACGCGCCGTTGTTGTTAA‑AACAACTGt                                                    >  2:1024393/1‑100 (MQ=255)
                                              cTGGCAGCAACACTGCGCGCAGCTGCGTGATGAACATTCCTGGCGTTACGACCATTCCCGGTGACGCTATCTACGCGCCGTTGTTGTTAA‑AACAACTGTc                                                   <  2:309604/100‑1 (MQ=255)
                                              cTGGCAGCAACACTGCGCGCAGCTGCGTGATGAACATTCCTGGCGTTACGACCATTCCCGGTGACGCTATCTACGCGCCGTTGTTGTTAA‑AACAACTGTc                                                   >  1:42735/1‑100 (MQ=255)
                                               tGGCAGCAACACTGCGCGCAGCTGCGTGATGAACATTCCTGGCGTTACGACCATTCCCGGTGACGCTATCTACGCGCCGTTGTT‑TTaacaacaacggcgc                                                  <  2:282610/100‑6 (MQ=255)
                                                ggCAGCAACACTGCGCGCAGCTGCGTGATGAACATTCCTGGCGTTACGACCATTCCCGGTGACGCTATCTACGCGCCGTTGTTGTTAA‑AACAACTGTCgg                                                 >  1:2310258/1‑100 (MQ=255)
                                                  cagcaACACTGCGCGCAGCTGCGTGATGAACATTCCTGGCGTTACGACCATTCCCGGTGACGCTATCTACGCGCCGTTGTTGTTAA‑AACAACTGTCGGAt                                               <  2:310508/100‑1 (MQ=255)
                                                  cagcaACACTGCGCGCAGCTGCGTGATGAACATTCCTGGCGTTACGACCA‑TCCCGGTGACGCTATCTACGCGCCGTTGTTGTTAA‑AACAACTGTCGGATc                                              <  2:2155828/100‑1 (MQ=255)
                                                    gcaACACTGCGCGCAGCTGCGTGATGAACATTCCTGGCGTTACGACCATTCCCGGTGACGCTATCTACGCGCCGTTGTTGTTAA‑AACAACTGTCGGATCg                                             <  1:1770796/100‑1 (MQ=255)
                                                     caACACTGCGCGCAGCTGCGTGATGAACATTCCTGGTGTTACGACCATTCCCGGTGACGCTATCTACGCGCCGTTGTTGTTAA‑AACAACTGTCGGATCGt                                            <  1:402692/100‑1 (MQ=255)
                                                      aaCACTGCGCGCAGCTGCGTGATGAACATTCCTGGCGTTACGACCATTCCCGGTGACGCTATCTACGCGCCGTTGTTGTTAA‑AACAACTGTCGGATCGTa                                           >  2:430072/1‑100 (MQ=255)
                                                      aaCACTGCGCGCAGCTGCGTGATGAACATTCCTGGCGTTACGACCATTCCCGGTGACGCTATCTACGCGCCGTTGTTGTTAA‑AACAACTGTCGGATCGTa                                           <  1:10882/100‑1 (MQ=255)
                                                       acacTGCGCGCAGCTGCGTGATGAACATTCCTGGCGTTACGACCA‑TCCCGGTGACGCTATCTACGCGCCGTTGTTGTTAA‑AACAACTGTCGGATCGTaaa                                         >  1:1010725/1‑100 (MQ=255)
                                                          cTGCGCGCAGCTGCGTGATGAACATTCCTGGCGTTACGACCATTCCCGGTGACGCTATCTACGCGCCGTTGTTGTTAA‑AACAACTGTCGGATCGTAAAcc                                       <  2:1576113/100‑1 (MQ=255)
                                                          cTGCGCGCAGCTGCGTGATGAACATTCCTGGCGTTACGACCATTCCCGGTGACGCTATCTACGCGCCGTTGTTGTTAA‑AACAACTGTCGGATCGTAAAcc                                       >  1:2272315/1‑100 (MQ=255)
                                                            gcgcgcAGCTGCGTGATGAACATTCCTGGCGTTACGACCATTCCCGGTGACGCTATCTACGCGCCGTTGTTGTTAA‑AACAACTGTCGGATCGTAAACCTg                                     >  1:2137256/1‑100 (MQ=255)
                                                              gcgcAGCTGCGTGATGAACATTCCTGGCGTTACGACCATTCCCGGTGACGCTATCTACGCGCCGTTGTTGTTAA‑AACAACTGTCGGATCGTAAACCTGCg                                   >  1:2792589/1‑100 (MQ=255)
                                                                 cAGCTGCGTGATGAACATTCCTGGCGTTACGACCATTCCCGGTGACGCTATCTACGCGCCGTTGTTGTTAA‑AACAACTGTCGGATCGTAAACCTGCGGAt                                <  1:907643/100‑1 (MQ=255)
                                                                   gCTGCGTGATGAACATTCCTGGCGTTACGACCATTCCCGGTGACGCTATCTACGCGCCGTTGTTGTTAA‑AACAACTGTCGGATCGTAAACCTGCGGATTg                              <  1:1854829/100‑1 (MQ=255)
                                                                     tGCGTGATGAACATTCCTGGCGTTACGGCCATTCCCGGTGACGCTATCTACGCGCCGTTGTTGTTAA‑ATCAACTGTCGGATCGTAAACCTGCGGATTGcg                            <  2:1071739/100‑1 (MQ=255)
                                                                     tGCGTGATGAACATTCCTGGCGTTACGACCATTCCCGGTGACGCTATCTACGCGCCGTTGTTGTTAA‑AACAACTGTCGGATCGTAAACCTGCGGATTGcg                            >  1:2242475/1‑100 (MQ=255)
                                                                     tGCGTGATGAACATTCCTGGCGTTACGACCATTCCCGGTGACGCTATCTACGCGCCGTTGTTGTTAA‑AACAACTGTCGGATCGTAAACCTGCGGATTGcg                            <  2:557629/100‑1 (MQ=255)
                                                                       cGTGATGAACTTTCCTGGCGTTACGACCATTCCCGGTGACGCTATCTACGCGCCGTTGTTGTTAA‑AACAACTGTCGGATCGTAAACCTGCGGATTGcgt                           <  2:2629189/99‑1 (MQ=255)
                                                                       cGTGATGAACATTCCTGGCGTTACGACCATTCCCGGTGACGCTATCTACGCGCCGTTGTTGTTAA‑AACAACTGTCGGATCGTAAACCTGCGGATTGcgtc                          <  1:692137/100‑1 (MQ=255)
                                                                        gTGATGAACATTCCTGGCGTTACGACCATTCCCGGTGACGCTATCTACGCGCCGTTGTTGTTAA‑AACAACTGTCGGATCGTAAACCTGCGGATTGcgtcg                         <  2:2367113/100‑1 (MQ=255)
                                                                          gatgaACATTCCTGGCGTTACGACCATTCCCGGTGACGCTATCTACGCGCCGTTGTTGTTAA‑AACAACTGTCGGATCGTAAACCTGCGGATTGCGTCGTg                       <  1:1162111/100‑1 (MQ=255)
                                                                          gatgaACATTCCTGGCGTTACGACCATTCCCGGTGACGCTATCTACGCGCCGTTGTTGTTAA‑AACAACTGTCGGATCGTAAACCTGCGGATTGCGTCGTg                       <  1:970120/100‑1 (MQ=255)
                                                                          gatgaACATTCCTGGCGTTACGACCA‑TCCCGGTGACGCTATCTACGCGCCGTTGTTGTTAA‑AACAACTGTCGGATCGTAAACCTGCGGATTGCGTCGTGa                      <  2:1010727/100‑1 (MQ=255)
                                                                           atgaACATTCCTGGCGTTACGACCATTCCCGGTGACGCTATCTACGCGCCGTTGTTGTTAA‑AACAACTGTCGGATCGTAAACCTGCGGATTGCGTCGTGa                      <  2:2792599/100‑1 (MQ=255)
                                                                           atgaACATTCCTGGCGTTACGACCATTCCCGGTGACGCTATCTACGCGCCGTTGTTGTTAA‑AACAACTGTCGGATCGTAAACCTGCGGATTGCGTCGTGa                      <  2:585264/100‑1 (MQ=255)
                                                                              aaCATTCCTGGCGTTACGACCATTCCCGGTGACGCTATCTACGCGCCGTTGTTGTTAA‑AACAACTGTCGGATCGTAAACCTGCGGATTGCGTCGTGACca                   <  2:2174866/100‑1 (MQ=255)
                                                                               aCATTCCTGGCGTTACGACCATTCCCGGTGACGCTATCTACGCGCCGTTGTTGTTAA‑AACAACTGTCGGATCGTAAACCTGCGGATTGCGTCGTGACcac                  >  2:1166377/1‑100 (MQ=255)
                                                                                cATTCCTGGCGTTACGACCATTCCCGGTGACGCTATCTACGCGCCGTTGTTGTTAA‑AACAACTGTCGGATCGTAAACCTGCGGCTTGCGTCGTCACcaca                 >  1:39865/1‑100 (MQ=255)
                                                                                  ttCCTGGCGTTACGACCATTCCCGGTGACGCTATCTACGCGCCGTTGTTGTTAA‑AACAACTGTCGGATCGTAAACCTGCGGATTGCGTCGTGACCACAGa               >  2:2444113/1‑100 (MQ=255)
                                                                                   ttcTGGCGTTACGACCATTCTCGGTGACGCTATCTACGCGCCGTTGTTGTTAA‑AACAACTGTCGGATCGTAAACCTGCGGATTGCGTCGTGAcc                    <  1:1516099/92‑1 (MQ=255)
                                                                                    ccTGGCGTTACGACCATTCCCGGTGACGCTATCTACGCGCCGTTGTTGTTAA‑AACAACTGTCGGATCGTAAACCTGCGGATTGCGTCGTGACCACAGAtg             <  2:2831428/100‑1 (MQ=255)
                                                                                    ccTGGCGTTACGACCATTCCCGGTGACGCTATCTACGCGCCGTTGTTGTTAA‑AACAACTGTCGGATCGTAAACCTGCGGATTGCGTCGTGACCACAGAtg             >  2:2866093/1‑100 (MQ=255)
                                                                                    ccTGGCGTTACGACCATTCCCGGTGACGCTATCTACGCGCCGTTGTTGTTAA‑AACAACTGTCGGATCGTAAACCTGCGGATTGCGTCGTGACCACAGAtg             <  2:2303062/100‑1 (MQ=255)
                                                                                     cTGGCGTTACGACCATTCCCGGTGACGCTATCTACGCGCCGTTGTTGTTAA‑AACAACTGTCGGATCGTAAACCTGCGGATTGCGTCGTGACCACAGAtgt            >  1:1187235/1‑100 (MQ=255)
                                                                                      tGGCGTTACGACCATTCCCGGTGACGCTATCTACGCGCCGTTGTTGTTAA‑AACAACTGTCGGATCGTAAACCTGCGGATTGCGTCGTGACCACAGAtgtg           >  2:1107875/1‑100 (MQ=255)
                                                                                       ggCGTTACGACCATTCCCGGTGACGCTATCTACGCGCCGTTGTTGTTAA‑AACAACTGTCGGATCGTAAACCTGCGGATTGCGTCGTGACCACAGATGTgg          <  1:2436237/100‑1 (MQ=255)
                                                                                       ggCGTTACGACCATTCCCGGTGACGCTATCTACGCGCCGTTGTTGTTAA‑AACAACTGTCGGATCGTAAACCTGCGGATTGCGTCGTGACCACAGATGTgg          <  2:1862328/100‑1 (MQ=255)
                                                                                        gCGTTACGACCATTCCCGGTGACGCTATCTACGCGCCGTTGTTGTTAA‑AACAACTGTCGGATCGTAAACCTGCGGATTGCGTCGTGACCACAGATGTggg         <  2:732463/100‑1 (MQ=255)
                                                                                         cGTTACGACCATTCCCGGTGACGCTATCTACGCGCCGTTGTTGTTAA‑AACAACTGTCGGATCGTAAACCTGCGGATTGCGTCGTGACCACAGATGTgggg        >  2:2218817/1‑100 (MQ=255)
                                                                                         cGTTACGACCATTCCCGGTGACGCTATCTACGCGCCGTTGTTGTTAA‑AACAACTGTCGGATCGTAAACCTGCGGATTGCGTCGTGACCACAGATGTggg         <  1:2794354/99‑1 (MQ=255)
                                                                                         cGTTACGACCATTCCCGGTGACGCTATCTACGCGCCGTTGTTGTTAA‑AACAACTGTCGGATCGTAAACCTGCGGATTGCGTCGTGACCACAGATGTggg         <  1:1067875/99‑1 (MQ=255)
                                                                                          gTTACGACCATTCCCGGTGACGCTATCTACGCGCCGTTGTTGTTAA‑AACAACTGTCGGATCGTAAACCTGCGGATTGCGTCGTGACCACAGATGTGGGgc       <  2:1393167/100‑1 (MQ=255)
                                                                                          gTTACGACCATTCCCGGTGACGCTATCTACGCGCCGTTGTTGTTAA‑AACAACTGTCGGATCGTAAACCTGCGGATTGCGTCGTGACCACAGATGTGGGgc       >  1:2828369/1‑100 (MQ=255)
                                                                                           ttACGACCATTCCCGGTGACGCTATCTACGCGCCGTTGTTGTTAA‑AACAACTGTCGGATCGTAAACCTGCGGATTGCGTCGTGACCACAGATGTGGGgca      <  2:522145/100‑1 (MQ=255)
                                                                                            tACGACCATTCCCGGTGACGCTATCTACGCGCCGTTGTTGTTAA‑AACAACTGTCGGATCGTAAACCTGCGGATTGCGTCGTGACCACAGATGTGGGgcag     >  2:201920/1‑100 (MQ=255)
                                                                                               gACCATTCCCGGTGACGCTATCTACGCGCCGTTGTTGTTAA‑AACAACTGTCGGATCGTAAACCTGCGGATTGCGTCGTGACCACAGATGTGGGGCAGCAc  <  2:702596/100‑1 (MQ=255)
                                                                                                    |                                                                                               
TTTAAATGCTCTGTTACCAGCATTACAGCAGCCGTTAAATCAATGACTGGCAGCAACACTGCGCGCAGCTGCGTGATGAACATTCCTGGCGTTACGACCA‑TCCCGGTGACGCTATCTACGCGCCGTTGTTGTTAA‑AACAACTGTCGGATCGTAAACCTGCGGATTGCGTCGTGACCACAGATGTGGGGCAGCAC  >  NC_000913/3951498‑3951691

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A