Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A10 F169 I1 R1 59 169.8 8365175 96.8% 8097489 99.9

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 3,951,598 +T pseudogene (57/663 nt) ilvG → pseudogene, acetolactate synthase 2 large subunit, valine‑insensitive; acetolactate synthase II, large subunit, cryptic, interrupted

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,951,5971.T97.7% 597.1 / 5.0 176pseudogene (56/663 nt)ilvGpseudogene, acetolactate synthase 2 large subunit, valine‑insensitive; acetolactate synthase II, large subunit, cryptic, interrupted
Reads supporting (aligned to +/- strand):  ref base . (2/2);  new base T (91/79);  total (93/83)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.98e-01

Only 100 of 188 total aligned reads displayed.

TTTAAATGCTCTGTTACCAGCATTACAGCAGCCGTTAAATCAATGACTGGCAGCAACACTGCGCGCAGCTGCGTGATGAACATTCCTGGCGTTACGACCA‑TCCCGGTGACGCTATCTACGCGCCGTTGTTGTTAA‑AACAACTGTCGGATCGTAAACCTGCGGATTGCGTCGTGACCACAGATGTGGGGCAGCA  >  NC_000913/3951498‑3951690
                                                                                                    |                                                                                              
tttAAATGCTCTGTTACCAGCATTACAGCAGCCGTTAAATCAATGACTGGCAGCAACACTGCGCGCAGCTGCGTGATGAACATTCCTGGCGTTACGACCa                                                                                                 >  2:3288816/1‑100 (MQ=255)
 ttAAATGCTCTGTTACCAGCATTACAGCAGCCGTTAAATCAATGACTGGCAGCAACACTGCGCGCAGCTGCGTGATGAACATTCCTGGCGTTACGACCA‑t                                                                                               <  1:2594954/100‑1 (MQ=255)
  tAAATGCTCTGTTACCAGCATTACAGCAGCCGTTAAATCAATGACTGGCAGCAACACTGCGCGCAGCTGCGTGATGAACATTCCTGGCGTTACGACCA‑tt                                                                                              >  2:2469518/1‑99 (MQ=255)
  tAAATGCTCTGTTACCAGCATTACAGCAGCCGTTAAATCAATGACTGGCAGCAACACTGCGCGCAGCTGCGTGATGAACATTCCTGGCGTTACGACCA‑tt                                                                                              >  1:456647/1‑99 (MQ=255)
  tAAATGCTCTGTTACCAGCATTACAGCAGCCGTTAAATCAATGACTGGCAGCAACACTGCGCGCAGCTGCGTGATGAACATTCCTGGCGTTACGACCA‑tt                                                                                              >  2:3576632/1‑99 (MQ=255)
     aTGCTCTGTTACCAGCATTACAGCAGCCGTTAAATCAATGACTGGCAGCAACACTGCGCGCAGCTGCGTGATGAACATTCCTGGCGTTACGACCA‑ttccc                                                                                           >  2:1157979/1‑96 (MQ=255)
      tGCTCTGTTACCAGCATTACAGCAGCCGTTAAATCAATGACTGGCAGCAACACTGCGCGCAGCTGCGTGATGAACATTCCTGGCGTTACGACCA‑Ttcccg                                                                                          <  1:3387469/100‑3 (MQ=255)
       gCTCTGTTACCAGCATTACAGCAGCCGTTAAATCAATGACTGGCAGCAACACTGCGCGCAGCTGCGTGATGAACATTCCTGGCGTTACGACCATTCCCgg                                                                                          <  2:2396064/100‑1 (MQ=255)
       gCTCTGTTACCAGCATTACAGCAGCCGTTAAATCAATGACTGGCAGCAACACTGCGCGCAGCTGCGTGATGAACATTCCTGGCGTTACGACCATTCCCgg                                                                                          <  1:3711540/100‑1 (MQ=255)
       gCTCTGTTACCAGCATTACAGCAGCCGTTAAATCAATGACTGGCAGCAACACTGCGCGCAGCTGCGTGATGAACATTCCTGGCGTTACGACCATTCCCgg                                                                                          >  1:2351690/1‑100 (MQ=255)
        ctctGTTACCAGCATTACAGCAGCCGTTAAATCAATGACTGGCAGCAACACTGCGCGCAGCTGCGTGATGAACATTCCTGGCGTTACGACCATTCCCgg                                                                                          >  2:2976577/1‑99 (MQ=255)
        ctctGTTACCAGCATTACAGCAGCCGTTAAATCAATGACTGGCAGCAACACTGCGCGCAGCTGCGTGATGAACATTCCTGGCGTTACGACCA‑ttccaggt                                                                                        <  2:2662770/100‑8 (MQ=255)
         tctGTTACCAGCATTACAGCAGCCGTTAAATCAATGACTGGCAGCAACACTGCGCGCAGCTGCGTGATGAACATTCCTGGCGTTACGACCATTCCCGGTg                                                                                        <  1:2762564/100‑1 (MQ=255)
          ctGTTACCAGCATTACAGCAGCCGTTAAATCAATGACTGGCAGCAACACTGCGCGCAGCTGCGTGATGAACATTCCTGGCGTTACGACCATTCCCGGTGa                                                                                       >  1:1095438/1‑100 (MQ=255)
          ctGTTACCAGCATTACAGCAGCCGTTAAATCAATGACTGGCAGCAACACTGCGCGCAGCTGCGTGATGAACATTCCTGGCGTTACGACCATTCCCGGTGa                                                                                       >  2:852483/1‑100 (MQ=255)
          ctGTTACCAGCAGTACAGCAGCCGTTAAATCAATGACTGGCAGCAACACTGCGCGCAGCTGCGTGATGAACATGCCTGGCGTTACGACCATTCCCGGt                                                                                         <  2:52882/98‑1 (MQ=255)
           tGTTACCAGCATTACAGCAGCCGTTAAATCAATGACTGGCAGCAACACTGCGCGCAGCTGCGTGATGAACATTCCTGGCGTTACGACCATTCCCGGTGan                                                                                      <  1:2045133/100‑2 (MQ=255)
           tGTTACCAGCATTACAGCAGCCGTTAAATCAATGACTGGCAGCAACACTGCGCGCAGCTGCGTGATGAACATTCCTGGCGTTACGACCATTCCCGGTGAc                                                                                      <  2:1419103/100‑1 (MQ=255)
           tGTTACCAGCATTACAGCAGCCGTTAAATCAATGACTGGCAGCAACACTGCGCGCAGCTGCGTGATGAACATTCCTGGCGTTACGACCATTCCCGGTGAc                                                                                      >  1:2038539/1‑100 (MQ=255)
           tGTTACCAGCATTACAGCAGCCGTTAAATCAATGACTGGCAGCAACACTGCGCGCAGCTGCGTGATGAACATTCCTGGCGTTACGACCATTCCCGGTGAc                                                                                      <  1:3163466/100‑1 (MQ=255)
              tACCAGCATTACAGCAGCCGTTAAATCAATGACTGGCAGCAACACTGCGCGCAGCTGCGTGATGAACATTCCTGGCGTTACGACCATTCCCGGTGACGCt                                                                                   >  2:2496642/1‑100 (MQ=255)
              tACCAGCATTACAGCAGCCGTTAAATCAATGACTGGCAGCAACACTGCGCGCAGCTGCGTGATGAACATTCCTGGCGTTACGACCATTCCCGGTGACGCt                                                                                   >  1:2178261/1‑100 (MQ=255)
               aCCAGCATTACAGCAGCCGTTAAATCAATGACTGGCAGCAACACTGCGCGCAGCTGCGTGATGAACATTCCTGGCGTTACGACCATTCCCGGTGACGCTa                                                                                  >  1:3835107/1‑100 (MQ=255)
                 cAGCATTACAGCAGCCGTTAAATCAATGACTGGCAGCAACACTGCGCGCAGCTGCGTGATGAACATTCCTGGCGTTACGACCATTCCCGGTGACGCTATc                                                                                <  1:2305998/100‑1 (MQ=255)
                 cAGCATTACAGCAGCCGTTAAATCAATGACTGGCAGCAACACTGCGCGCAGCTGCGTGATGAACATTCCTGGCGTTACGACCATTCCCGGTGACGCTATc                                                                                >  1:930421/1‑100 (MQ=255)
                 cAGCATTACAGCAGCCGTTAAATCAATGACTGGCAGCAACACTGCGCGCAGCTGCGTGATAAACATTGCTGGCGTTACGACCATTCCCGGTGACGCTATc                                                                                >  2:2938643/1‑100 (MQ=255)
                  aGCATTACAGCAGCCGTTAAATCAATGACTGGCAGCAACACTGCGCGCAGCTGCGTGATGAACATTCCTGGCGTTACGACCATTCCCGGTGACGCTATCt                                                                               <  2:3752497/100‑1 (MQ=255)
                   gCATTACAGCAGCCGTTAAATCAATGACTGGCAGCAACACTGCGCGCAGCTGCGTGATGAACATTCCTGGCGTTACGACCATTCCCGGTGACGCTATCTa                                                                              >  1:1832512/1‑100 (MQ=255)
                   gCATTACAGCAGCCGTTAAATCAATGACTGGCAGCAACACTGCGCGCAGCTGCGTGATGAACATTCCTGGCGTTACGACCATTCCCGGTGACGCTATCTa                                                                              <  1:873396/100‑1 (MQ=255)
                    cATTACAGCAGCCGTTAAATCAATGACTGGCAGCAACACTGCGCGCAGCTGCGTGATGAACATTCCTGGCGTTACGACCATTCCCGGTGACGCTATCTAc                                                                             >  2:3331341/1‑100 (MQ=255)
                       tACAGCAGCCGTTAAATCAATGACTGGCAGCAACACTGCGCGCAGCTGCGTGATGAACATTCCTGGCGTTACGACCATTCCCGGTGACGCTATCTAcgcg                                                                          <  1:3027150/100‑1 (MQ=255)
                           gcagcCGTTAAATCAATGACTGGCAGCAACACTGCGCGCAGCTGCGTGATGAACATTCCTGGCGTTACGACCATTCCCGGTGACGCTATCTACGCGCCgt                                                                      >  1:2960063/1‑100 (MQ=255)
                            cagcCGTTAAATCAATGACTGGCAGCAACACTGCGCGCAGCTGCGTGATGAACATTCCTGGCGTTACGACCATTCCCGGTGACGCTATCTACGCGCCgt                                                                      <  2:33572/99‑1 (MQ=255)
                              gcCGTTAAATCAATGACTGGCAGCAACACTGCGCGCAGCTGCGTGATGAACATTCCTGGCGTTACGACCATTCCCGGTGACGCTATCTACGCGCCgttgt                                                                   <  1:196017/100‑1 (MQ=255)
                              gcCGTTAAATCAATGACTGGCAGCAACACTGCGCGCAGCTGCGTGATGAACATTCCTGGCGTTACGACCATTCCCGGTGACGCTATCTACGCGCCgttgt                                                                   >  1:1684044/1‑100 (MQ=255)
                              gcCGTTAAATCAATGACTGGCAGCAACACTGCGCGCAGCTGCGTGATGAACATTCCTGGCGTTACGACCATTCCCGGTGACGCTATCTACGCGCCgttgt                                                                   >  1:4153031/1‑100 (MQ=255)
                              gcCGTTAAATCAATGACTGGCAGCAACACTGCGCGCAGCTGCGTGATGAACATTCCTGGCGTTACGACCA‑TCCCGGTGACGCTATCTACGCGCCgttgtt                                                                  >  2:3997557/1‑100 (MQ=255)
                               ccGTTAAATCAATGACTGGCAGCAACACTGCGCGCAGCTGCGTGATGAACATTCCTGGCGTTACGACCATTCCCGGTGACGCTATCTACGCGCCgttgtt                                                                  <  2:2591757/100‑1 (MQ=255)
                                cGTTAAATCAATGACTGGCAGCAACACTGCGCGCAGCTGCGTGATGAACATTCCTGGCGTTACGACCATTCCCGGTGACGCTATCTACGCGCCgttgttg                                                                 >  2:3100675/1‑100 (MQ=255)
                                cGTTAAATCAATGACTGGCAGCAACACTGCGCGCAGCTGCGTGATGAACATTCCTGGCGTTACGACCATTCCCGGTGACGCTATCTACGCGCCgttgttg                                                                 >  2:471981/1‑100 (MQ=255)
                                 gTTAAATCAATGACTGGCAGCAACACTGCGCGCCGCTGCGTGATGAACATTCCTGGCGTTACGACCATTCCCGGTGACGCTATCTACGCGCCgttgttg                                                                 <  2:3906657/99‑1 (MQ=255)
                                 gTTAAATCAATGACTGGCAGCAACACTGCGCGCAGCTGCGTGATGAACATTCCTGGCGTTACGACCATTCCCGGTGACGCTATCTACGCGCCgttgttgt                                                                <  2:506773/100‑1 (MQ=255)
                                  ttAAATCAATGACTGGCAGCAACACTGCGCGCAGCTGCGTGATGAACATTCCTGGCGTTACGACCATTCCCGGTGACGCTATCTACGCGCCgttgttgtt                                                               <  2:227475/100‑1 (MQ=255)
                                   tAAATCAATGACTGGCAGCAACACTGCGCGCAGCTGCGTGATGAACATTCCTGGCGTTACGACCATTCCCGGTGACGCTATCTACGCGCCGTTGTTGTTa                                                              >  2:2006268/1‑100 (MQ=255)
                                   tAAATCAATGACTGGCAGCAACACTGCGCGCAGCTGCGTGATGAACATTCCTGGCGTTACGACCATTCCCGGTGACGCTATCTACGCGCCGTTGTTGTTa                                                              <  2:147196/100‑1 (MQ=255)
                                    aaaTCAATGACTGGCAGCAACACTGCGCGCAGCTGCGTGATGAACATTCCTGGCGTTACGACCATTCCCGGTGACGCTATCTACGCGCCGTTGTTGTTaa                                                             >  1:1144679/1‑100 (MQ=255)
                                     aaTCAATGACTGGCAGCAACACTGCGCGCAGCTGCGTGATGAACATTCCTGGCGTTACGACCATTCCCGGTGACGCTATCTACGCGCCGTTGTTGTTaa‑a                                                           >  1:1883955/1‑100 (MQ=255)
                                      aTCAATGACTGGCAGCAACACTGCGCGCAGCTGCGTGATGAACATTCCTGGCGTTACGACCATTCCCGGTGACGCTATCTACGCGCCGTTGTTGTTaa‑aa                                                          >  2:3746968/1‑100 (MQ=255)
                                       tCAATGACTGGCAGCAACACTGCGCGCAGCTGCGTGATGAACATTCCTGGCGTTACGACCATTCCCGGTGACGCTATCTACGCGCCGTTGTTGTTAA‑aac                                                         <  2:1213603/100‑1 (MQ=255)
                                       tCAATGACTGGCAGCAACACTGCGCGCAGCTGCGTGATGAACATTCCTGGCGTTACGACCATTCCCGGTGACGCTATCTACGCGCCGTTGTTGTTAA‑aac                                                         <  2:317664/100‑1 (MQ=255)
                                       tCAATGACTGGCAGCAACACTGCGCGCAGCTGCGTGATGAACATTCCTGGCGTTACGACCATTCCCGGTGACGCTATCTACGCGCCGTTGTTGTTAA‑aac                                                         <  2:436363/100‑1 (MQ=255)
                                            gACTGGCAGCAACACTGCGCGCAGCTGCGTGATGAACATTCCTGGCGTTACGACCATTCCCGGTGACGCTATCTACGCGCCGTTGTTGTTAA‑AACAACTg                                                    >  2:1224901/1‑100 (MQ=255)
                                            gACTGGCAGCAACACTGCGCGCAGCTGCGTGATGAACATTCCTGGCGTTACGACCATTCCCGGTGACGCTATCTACGCGCCGTTGTTGTTAA‑AACAACTg                                                    <  1:3288851/100‑1 (MQ=255)
                                             aCTGGCAGCAACACTGCGCGCAGCTGCGTGATGAACATTCCTGGCGTTACGACCATTCCCGGTGACGCTATCTACGCGCCGTTGTTGTTAA‑AACAACTGt                                                   >  2:4083411/1‑100 (MQ=255)
                                              cTGGCAGCAACACTGCGCGCAGCTGCGTGTTGAACATTCCTGGCGTTACGACCATTCCCGGTGACGCTATCTACGCGCCGTTGTTGTTAA‑AACAACTGTc                                                  <  2:1562615/100‑1 (MQ=255)
                                              cTGGCAGCAACACTGCGCGCAGCTGCGTGATGAACATTCCTGGCGTTACGACCATTCCCGGTGACGCTATCTACGCGCCGTTGTTGTTAA‑AACAACTGTc                                                  >  1:3884791/1‑100 (MQ=255)
                                              cTGGCAGCAACACTGCGCGCAGCTGCGTGATGAACATTCCTGGCGTTACGACCATTCCCGGTGACGCTATCTACGCGCCGTTGTT‑TTaacaacaacggcg                                                  <  1:1285693/100‑5 (MQ=255)
                                                ggCAGCAACACTGCGCGCAGCTGCGTGATGAACATTCCTGGCGTTACGACCATTCCCGGTGACGCTACCTACGCGCCGTTGTTGTTAC‑AACAACTGTCgg                                                <  1:2746191/100‑1 (MQ=255)
                                                 gcagcaACACTGCGCGCAGCTGCGTGATGAACTTTCCTGGCGTTACGACCATTCCCGGTGACGCTATCTACGCGCCGTTGTTGTTAA‑AACAACTGTCg                                                 <  2:430166/98‑1 (MQ=255)
                                                  cagcaACACTGCGCGCAGCTGCGTGATGAACATTCCTGGCGTTACGACCATTCCCGGTGACGCTATCTACGCGCCGTTGTTGTTAA‑AACAACTGTCGGAt                                              >  1:3506882/1‑100 (MQ=255)
                                                   agcaACACTGCGCGCAGCTGCGTGATGAACATTCCTGGCGTTACGACCATTCCCGGTGACGCTATCTACGCGCCGTTGTTGTTAA‑AACAACTGTCGGATc                                             >  2:2162357/1‑100 (MQ=255)
                                                    gcaACACTGCGCGCAGCTGCGTGATGAACATTCCTGGCGTTACGACCATTCCCGGTGACGCTATCTACGCGCCGTTGTTGTTAA‑AACAACTGTCGGATCg                                            >  1:3572787/1‑100 (MQ=255)
                                                     caACACTGCGCGCAGCTGCGTGATGAACATTCCTGGCGTTACGACCATTCCCGGTGACGCTATCTACGCGCCGTTGTTGTTAA‑AACAACTGTCGGATCGt                                           <  2:11337/100‑1 (MQ=255)
                                                      aaCACTGCGCGCAGCTTCGTGATGAACATTCCTGGCGTTACGACCATTCCCGGTGACGCTATCTACGCGCCGTTGTTGTTAA‑AACAACTGTCGGATCGTa                                          <  2:1088567/100‑1 (MQ=255)
                                                      aaCACTGCGCGCAGCTGCGTGATGAACATTCCTGGCGTTACGACCATTCCCGGTGACGCTATCTACGCGCCGTTGTTGTTAA‑AACAACTGTCGGATCGTa                                          >  1:2466847/1‑100 (MQ=255)
                                                        cacTGCGCGCAGCTGCGTGATGAACATTCCTGGCGTTACGACCATTCCCGGTGACGCTATCTACGCGCCGTTGTTGTTAA‑AACAACTGTCGGATCGTaaa                                        >  1:3925040/1‑100 (MQ=255)
                                                          cTGCGCGCAGCTGCGTGATGAACATTCCTGGCGTTACGACCATTCCCGGTGACGCTATCTACGCGCCGTTGTTGTTAA‑AACAACTGTCGGATCGTAAAcc                                      >  2:3385447/1‑100 (MQ=255)
                                                             cgcgcAGCTGCGTGATGAACATTCCTGGCGTTACGACCATTCCCGGTGACGCTATCTACGCGCCGTTGTTGTTAA‑AACAACTGTCGGATCGTAAACCTGc                                   >  1:4103530/1‑100 (MQ=255)
                                                              gcgcAGCTGCGTGATGAACATTCCTGGCGTTACGACCATTCCCGGTGACGCTATCTACGCGCCGTTGTTGTTAA‑AACAACTGTCGGATCGTAAACCTGCg                                  <  2:3262499/100‑1 (MQ=255)
                                                               cgcAGCTGCGTGATGAACATTCCTGGCGTTACGACCATTCCCGGTGACGCTATCTACGCGCCGTTGTTGTTAA‑AACAACTGTCGGATCGTAAACCTGCgg                                 <  2:3805104/100‑1 (MQ=255)
                                                                 cAGCTGTGTGATGAACATTCCTGGCGTTACGACCATTCCCGGTGACGCTATCTAGGCGCCGTTGTTGTTAA‑AACAACTGTCGGATCGTAAACCTGCGGAt                               >  1:3787527/1‑100 (MQ=255)
                                                                  aGCTGCGTGATGAACATTCCTGGCGTTACGACCATTCCCGGTGACGCTATCTACGCGCCGTTGTTGTTAA‑AACAACTGTCGGATCGTAAACCTGCGGAtt                              >  1:760035/1‑100 (MQ=255)
                                                                   gCTGCGTGATGAACATTCCTGGCGTTACGACCATTCCCGGTGACGCTATCTACGCGCCGTTGTTGTTAA‑AACAACTGTCGGATCGTAAACCTGCGGATTg                             <  2:3847843/100‑1 (MQ=255)
                                                                        gTGATGAACATTCCTGGCGTTACGACCATTCCCGGTGACTCTATCTACGCGCCGTTGTTGTTAA‑AACAACTGTCGGATCGTAAACCTGCGGATTGcgtcg                        >  1:3319997/1‑100 (MQ=255)
                                                                        gTGATGAACATTCCTGGCGTTACGACCATTCCCGGTGACGCTATCTACGCGCCGTTGTTGTTAA‑AACAACTGTCGGATCGTAAACCTGCGGATTGtgtcg                        <  1:2080955/100‑1 (MQ=255)
                                                                         tgatgaACATTCCTGGCGTTACGACCATTCCCGGTGACGCTATCTACGCGCCGTTGTTGTTAA‑AACAACTGTCGGATCGTAAACCTGCGGATTGcgtcgt                       <  2:2351667/100‑1 (MQ=255)
                                                                           atgaACATTCCTGGCGTTACGACCATTCCCGGTGACGCTATCTACGCGCCGTTGTTGTTAA‑AACAACTGTCGGATCGTAAACCTGCGGATTGCGTCGTGa                     <  2:3106332/100‑1 (MQ=255)
                                                                           atgaACATTCCTGGCGTTACGACCATTCCCGGTGACGCTATCTACGCGCCGTTGTTGTTAA‑AACAACTGTCGGATCGTAAACCTGCGGATTGCGTCGTGa                     >  2:67109/1‑100 (MQ=255)
                                                                           atgaACATTCCTGGCGTTACGACCATTCCCGGTGACGCTATCTACGCGCCGTTGTTGTTAA‑AACAACTGTCGGATCGTAAACCTGCGGATTGCGTCGTGa                     <  2:3835064/100‑1 (MQ=255)
                                                                           atgaACATTCCTGGCGTTACGACCAGTCCCGGTGACGCTATCTACGCGCCGTTGTTGTTAA‑AACAACTGTCGGATCGTAAACCTGCGGATTGCGTCGTGa                     <  2:3624501/100‑1 (MQ=255)
                                                                            tgaACATTCCTGGCGTTACGACCATTCCCGGTGACGCTATCTACGCGCCGTTGTTGTTAA‑AACAACTGTCGGATCGTAAACCTGCGGATTGCGTCGTGAc                    >  2:3019064/1‑100 (MQ=255)
                                                                            tgaACATTCCTGGCGTTACGACCATTCCCGGTGACGCTATCTACGCGCCGTTGTTGTTAA‑AACAACTGTCGGATCGTAAACCTGCGGATTGCGTCGTGAc                    >  2:1072181/1‑100 (MQ=255)
                                                                            tgaACATTCCTGGCGTTACGACCATTCCCGGTGACGCTATCTACGCGCCGTTGTTGTTAA‑AACAACTGTCGGATCGTAAACCTGCGGATTGCGTCGTGAc                    >  1:1496281/1‑100 (MQ=255)
                                                                             gaACATTCCTGGCGTTACGACCATTCCCGGTGACGCTATCTACGCGCCGTTGTTGTTAA‑AACACCTGTCGGATCGTAAACCTGCGGATTGCGTCGTGAcc                   <  1:2938675/100‑1 (MQ=255)
                                                                             gaACATTCCTGGCGTTACGACCATTCCCGGTGACGCTATCTACGCGCCGTTGTTGTTAA‑AACAACTGTCGGATCGTAAACCTGCGGATTGCGTCGTGAcc                   <  2:1013537/100‑1 (MQ=255)
                                                                             gaACATTCCTGGCGTTACGACCATTCCCGGTGACGCTATCTACGCGCCGTTGTTGTTAA‑AACAACTGTCGGATCGTAAACCTGCGGATTGCGTCGTGAcc                   >  1:1762806/1‑100 (MQ=255)
                                                                              aaCATTCCTGGCGTTACGACCATTCCCGGTGACGCTATCTACGCGCCGTTGTTGTTAA‑AACAACTGTCGGATCGTAAACCTGCGGATTGCGTCGTg                      >  2:1171345/1‑96 (MQ=255)
                                                                               aCATTCCTGGCGTTACGACCATTCCCGGTGACGCTATCTACGCGCCGTTGTTGTTAA‑AACAACTGTCGGATCGTAAACCTGCGGATTGCGTCGTGACcan                 <  2:2055826/100‑2 (MQ=255)
                                                                               aCATTCCTGGCGTTACGACCATTCCCGGTGACGCTATCTACGCGCCGTTGTTGTTAA‑AACAACTGTCGGATCGTAAACCTGCGGATTGCGTCGTGACcac                 <  2:1739065/100‑1 (MQ=255)
                                                                                  ttCCTGGCGTTACGACCATTCCCGGTGACGCTATCTACGCGCCGTTGTTGTTAA‑AACAACTGTCGGATCGTAAACCTGCGGATTGCGTCGTGACCACAGa              <  1:977128/100‑1 (MQ=255)
                                                                                    ccTGGCGTTACGACCATTCCCGGTGACGCTATCTACGCGCCGTTGTTGTTAA‑AACAACTGTCGGATCGTAAACCTGCGGATTGCGTCGTGACCACAGAtg            >  1:3675713/1‑100 (MQ=255)
                                                                                     cTGGCGTTACGACCATTCCCGGTGACGCTATCTACGCGCCGTTGTTGTTAA‑AACAACTGTCGGATCGTAAACCTGCGGATTGTGTCGTGACCACAGAtgt           <  2:3528416/100‑1 (MQ=255)
                                                                                       ggCGTTACGACCATTCCCGGTGACGCTATCTACGCGCCGTTGTTGTTAA‑AACAACTGTCGGATCGTAAACCTGCGGATTGCGTCGTGACCACAGATGTgg         >  1:3593509/1‑100 (MQ=255)
                                                                                        gCGTTACGACCATTCCCGGTGACGCTATCTACGCGCCGTTGTTGTTAA‑AACAACTGTCGGATCGTAAACCTGCTGATTGCGTCGTGACCACAGATGTggg        <  1:2255561/100‑1 (MQ=255)
                                                                                        gCGTTACGACCATTCCCGGTGACGCTATCTACGCGCCGTTGTTGTTAA‑AACAACTGTCGGATCGTAAACCTGCGGATTGCGTCGTGACCACAGATGTggg        <  2:1765198/100‑1 (MQ=255)
                                                                                          gTTACGACCATTCCCGGTGACGCTATCTACGCGCCGTTGTTGTTAA‑AACACCTGTCGGATCGTAAACCTGCGGATTGCGTCGTGACCACAGATGTGGGgc      >  1:2482087/1‑100 (MQ=255)
                                                                                            tACGACCATTCCCGGTGACGCTATCTACGCGCCGTTGTTGTTAA‑AACAACTGTCGGATCGTAAACCTGCGGATTGCGTCGTGACCACAGATGTGGGgcag    >  2:2624987/1‑100 (MQ=255)
                                                                                            tACGACCATTCCCGGTGACGCTATCTACGCGCCGTTGTTGTTAA‑AACAACTGTCGGATCGTAAACCTGCGGATTGCGTCGTGACCACAGATGTGGGgcag    <  2:938438/100‑1 (MQ=255)
                                                                                             aCGACCATTCCCGGTGACGCTATCTACGCGCCGTTGTTGTTAA‑AACAACTGTCGGATCGTAAACCTGCGGATTGCGTCGTGACCACAGATGTGGGgcagc   >  2:785130/1‑100 (MQ=255)
                                                                                              cGACCATTCCCGGTGACGCTATCTACGCGCCGTTGTTGTTAA‑AACAACTGTCGGATCGTAAACCTGCGGATTGCGTCGTGACCACAGATGTGGGgcagca  >  2:1957477/1‑100 (MQ=255)
                                                                                                    |                                                                                              
TTTAAATGCTCTGTTACCAGCATTACAGCAGCCGTTAAATCAATGACTGGCAGCAACACTGCGCGCAGCTGCGTGATGAACATTCCTGGCGTTACGACCA‑TCCCGGTGACGCTATCTACGCGCCGTTGTTGTTAA‑AACAACTGTCGGATCGTAAACCTGCGGATTGCGTCGTGACCACAGATGTGGGGCAGCA  >  NC_000913/3951498‑3951690

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A