Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A10 F210 I1 R1 75 123.0 6181441 93.9% 5804373 99.9

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 3,951,598 +T pseudogene (57/663 nt) ilvG → pseudogene, acetolactate synthase 2 large subunit, valine‑insensitive; acetolactate synthase II, large subunit, cryptic, interrupted

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,951,5971.T97.4% 395.7 / 2.8 116pseudogene (56/663 nt)ilvGpseudogene, acetolactate synthase 2 large subunit, valine‑insensitive; acetolactate synthase II, large subunit, cryptic, interrupted
Reads supporting (aligned to +/- strand):  ref base . (0/3);  new base T (57/56);  total (57/59)
Fisher's exact test for biased strand distribution p-value = 2.44e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.96e-01

Only 100 of 118 total aligned reads displayed.

TAAATGCTCTGTTACCAGCATTACAGCAGCCGTTAAATCAATGACTGGCAGCAACACTGC‑GCGCAGCTGCGTGATGAACATTCCTGGCGTTACGACCA‑TCCCGGTGACGCTATCTACGCGCCGTTGTTGTTAA‑AACAACTGTCGGATCGTAAACCTGCGGATTGCGTCGTGACCACAGATGTGGGGCAGCACCAGAT  >  NC_000913/3951500‑3951696
                                                                                                   |                                                                                                    
tAAATGCTCTGTTACCAGCATTACAGCAGCCGTTAAATCAATGACTGGCAGCAACACTGC‑GCGCAGCTGCGTGATGAACATTCCTGGCGTTACGACCA‑tt                                                                                                    <  1:3030380/100‑2 (MQ=255)
  aaTGCTCTGTTACCAGCATTACAGCAGCCGTTAAATCAATGACTGGCAGCAACACTGC‑GCGCAGCTGCGTGATGAACATTCCTGGCGTTACGACCA‑Ttcc                                                                                                  <  1:87832/100‑1 (MQ=255)
     gCTCTGTTACCAGCATTACAGCAGCCGTTAAATCAATGACTGGCAGCAACACTGC‑GCGCAGCTGCGTGATGAACATTCCTGGCGTTACGACCATTCCCgg                                                                                                <  2:340600/100‑1 (MQ=255)
     gCTCTGTTACCAGCATTACAGCAGCCGTTAAATCAATGACTGGCAGCAACACTGC‑GCGCAGCTGCGTGATGAACATTCCTGGCGTTACGACCATTCCCgg                                                                                                <  2:1666172/100‑1 (MQ=255)
     gCTCTGTTACCAGCATTACAGCAGCCGTTAAATCAATGACTGGCAGCAACACTGC‑GCGCAGCTGCGTGATGAACATTCCTGGCGTTACGACCATTCCCgg                                                                                                <  1:2053329/100‑1 (MQ=255)
      ctctGTTACCAGCATTACAGCAGCCGTTAAATCAATGACTGGCAGCAACACTGC‑GCGCAGCTGCGTGATGAACATTCCTGGCGTTACGACCATTCCCGGt                                                                                               >  1:501314/1‑100 (MQ=255)
      ctctGTTACCAGCATTACAGCAGCCGTTAAATCAATGACTGGCAGCAACACTGC‑GCGCAGCTGCGTGATGAACATTCCTGGCGTTACGACCATTCCCGGt                                                                                               >  1:2474889/1‑100 (MQ=255)
       tctGTTACCAGCATTACAGCAGCCGTTAAATCAATGACTGGCAGCAACACTGC‑GCGCAGCTGCGTGATGAACGTTCCTGGCGTTACGACCATTCCCGGTg                                                                                              <  2:600259/100‑1 (MQ=255)
       tctGTTACCAGCATTACAGCAGCCGTTAAATCAATGACTGGCAGCAACACTGC‑GCGCAGCTGCGTGATGAACATTCCTGGCGTTACGACCATTCCCgg                                                                                                <  1:2649535/98‑1 (MQ=255)
       tctGTTACCAGCATTACAGCAGCCGTTAAATCAATGACTGGCAGCAACACTGC‑GCGCAGCTGCGTGATGAACATTCCTGGCGTTACGACCATTCCCGGTg                                                                                              >  2:1518111/1‑100 (MQ=255)
        ctGTTACCAGCATTACAGCAGCCGTTAAATCAATGACTGGCAGCAACACTGC‑GCGCAGCTGCGTGATGAACATTCCTGGCGTTACGACCATTCCCGGTGa                                                                                             >  1:1046192/1‑100 (MQ=255)
         tGTTACCAGCATTACAGCAGCCGTTAAATCAATGACTGGCAGCAACACTGC‑GCGCAGCTGCGTGATGAACATTCCTGGCGTTACGACCATTCCCGGTGAc                                                                                            <  1:1313010/100‑1 (MQ=255)
          gTTACCAGCATTACAGCAGCCGTTAAATCAATGACTGGCCGCACCACTGC‑GCGCAGCTGCGTGATGAACATTCCTGGCGTTACGACCATTCCCGGTGACg                                                                                           >  2:2855825/1‑100 (MQ=255)
          gTTACCAGCATTACAGCAGCCGTTAAATCAATGACTGGCAGCAACACTGC‑GCGCAGCTGCGTGATGAACATTCCTGGCGTTACGACCATTCCCGGTGAc                                                                                            >  2:2690584/1‑99 (MQ=255)
             aCCAGCATTACAGCAGCCGTTAAATCAATGACTGGCAGCAACACTGC‑GCGCAGCTGCGTGATGAACATTCCTGGCGTTACGACCATTCCCGGTGACGCTa                                                                                        >  1:2524739/1‑100 (MQ=255)
              ccAGCATTACAGCAGCCGTTAAATCAATGACTGGCAGCAACACTGC‑GCGCAGCTGCGTGATGAACATTCCTGGCGTTACGACCATTCCCGGTGACGCTAt                                                                                       <  2:501313/100‑1 (MQ=255)
              ccAGCATTACAGCAGCCGTTAAATCAATGACTGGCAGCAACACTGC‑GCGCAGCTGCGTGATGAACATTCCTGGCGTTACGACCATTCCCGGTGACGCTAt                                                                                       >  2:2847856/1‑100 (MQ=255)
               cAGCATTACAGCAGCCGTTAAATCAATGACTGGCAGCAACACTGC‑GCGCAGCTGCGTGATGAACATTCCTGGCGTTACGACCATTCCCGGTGACGCTATc                                                                                      <  1:2853730/100‑1 (MQ=255)
               cAGCATTACAGCAGCCGTTAAATCAATGACTGGCAGCAACACTGC‑GCGCAGCTGCGTGATGAACATTCCTGGCGTTACGACCATTCCCGGTGACGCTATc                                                                                      <  1:2107235/100‑1 (MQ=255)
               cAGCATTACAGCAGCCGTTAAATCAATGACTGGCAGCAACACTGC‑GCGCAGCTGCGTGATGAACATTCCTGGCGTTACGACCATTCCCGGTGACGCTATc                                                                                      >  1:1835855/1‑100 (MQ=255)
                aGCATTACAGCAGCCGTTAAATCAATGACTGGCAGCAACACTGC‑GCGCAGCTGCGTGATGAACATTCCTGGCGTTACGACCATTCCCGGTGACGCTATCt                                                                                     >  1:151904/1‑100 (MQ=255)
                aGCATTACAGCAGCCGTTAAATCAATGACTGGCAGCAACACTGC‑GCGCAGCTGCGTGATGAACATTCCTGGCGTTACGACCATTCCCGGTGACGCTATCt                                                                                     <  2:267770/100‑1 (MQ=255)
                 gCATTACAGCAGCCGTTAAATCAATGACTGGCAGCAACACTGC‑GCGCAGCTGCGTGATGAACATTCCTGGCGTTACGACCATTCCCGGTGACGCTATCTa                                                                                    >  2:318692/1‑100 (MQ=255)
                   aTTACAGCAGCCGTTAAATCAATGACTGGCAGCAACACTGC‑GCGCAGCTGCGTGATGAACATTCCTGGCGTTACGACCATTCCCGGTGACGCTATCTAcg                                                                                  <  1:1183502/100‑1 (MQ=255)
                     tACAGCAGCCGTTAAATCAATGACTGGCAGCAACACTGC‑GCGCAGCTGCGTGATGAACATTCCTGGCGTTACGACCATTCCCGGTGACGCTATCTAcgcg                                                                                >  2:2635035/1‑100 (MQ=255)
                       cagcagCCGTTAAATCAATGACTGGCAGCAACACTGC‑GCGCAGCTGCGTGATGAACATTCCTGGCGTTACGACCATTCCCGGTGACGCTATCTACGCGcc                                                                              >  1:2154394/1‑100 (MQ=255)
                         gcagcCGTTAAATCAATGACTGGCAGCAACACTGC‑GCGCAGCTGCGTGATGAACATTCCTGGCGTTACGACCATTCCCGGTGACGCTATCTACGCGCCgt                                                                            >  2:158931/1‑100 (MQ=255)
                         gcagcCGTTAAATCAATGACTGGCAGCAACACTGC‑GCGCAGCTGCGTGATGAACATTCCTGGCGTTACGACCATTCCCGGTGACGCTATCTACGCGCCgt                                                                            <  1:1823698/100‑1 (MQ=255)
                          cagcCGTTAAATCAATGACTGGCAGCAACACTGC‑GCGCAGCTGCGTGATGAACATTCCTGGCGTTACGACCATTCCCGGTGACGCTATCTACGCGCCgtt                                                                           <  1:1992877/100‑1 (MQ=255)
                             ccGTTAAATCAATGACTGGCAGCAACACTGC‑GCGCAGCTGCGTGATGAACATTCCTGGCGTTACGACCATTCCCGGTGACGCTATCTACGCGCCgttgtt                                                                        <  2:112375/100‑1 (MQ=255)
                               gTTAAATCAATGACTGGCAGCAACACTGC‑GCGCAGCTGCGTGATGAACATTCCTGGCGTTACGACCATTCCCGGTGACGCTATCTACGCGCCgttgttgt                                                                      <  2:3013343/100‑1 (MQ=255)
                                 tAAATCAATGACTGGCAGCAACACTGC‑GCGCAGCTGCGTGATGAACATTCCTGGCGTTACGACCATTCCCGGTGACGCTATCTACGCGCCGTTGTTGTTa                                                                    <  1:2948974/100‑1 (MQ=255)
                                   aaTCAATGACTGGCAGCAACACTGC‑GCGCAGCTGCGTGATGAACATTCCTGGCGTTACGACCATTCCCGGTGACGCTATCTACGCGCCGTTGTTGTTaa‑a                                                                 >  2:1238632/1‑100 (MQ=255)
                                   aaTCAATGACTGGCAGCAACACTGC‑GCGCAGCTGCGTGATGAACATTCCTGGCGTTACGACCATTCCCGGTGACGCTATCTACGCGCCGTTGTTGTTaa‑a                                                                 <  1:2244359/100‑1 (MQ=255)
                                    aTCAATGACTGGCAGCAACACTGC‑GCGCATTTGCGTGATGAACATTCCTGGCGTTACGACCATTCCCGGTGACGCTATCTACGCGCCGTTGTTGTTaa‑aa                                                                <  2:1012798/100‑1 (MQ=255)
                                    aTCAATGACTGGCAGCAACACTGC‑GCGCAGCTGCGTGATGAACATTCCTGGCGTTACGACCATTCCCGGTGACGCTATCTACGCGCCGTTGTTGTTaa‑aa                                                                <  2:1866083/100‑1 (MQ=255)
                                    aTCAATGACTGGCAGCAACACTGC‑GCGCAGCTGCGTGATGAACATTCCTGGCGTTACGACCATTCCCGGTGACGCTATCTACGCGCCGTTGTTGTTaa‑aa                                                                <  2:632663/100‑1 (MQ=255)
                                    aTCAATGACTGGCAGCAACACTGC‑GCGCAGCTGCGTGATGAACATTCCTGGCGTTACGACCATTCCCGGTGACGCTATCTACGCGCCGTTGTTGTTaa‑aa                                                                <  2:1454176/100‑1 (MQ=255)
                                     tCAATGACTGGCAGCAACACTGC‑GCGCAGCTGCGTGATGAACATTCCTGGCGTTACGACCATTCCCGGTGACGCTATCTACGCGCCGTTGTTGTTAA‑aac                                                               >  1:2456015/1‑100 (MQ=255)
                                     tCAATGACTGGCAGCAACACTGC‑GCGCAGCTGCGTGATGAACATTCCTGGCGTTACGACCATTCCCGGTGACGCTATCTACGCGCCGTTGTTGTTAA‑aac                                                               <  1:195287/100‑1 (MQ=255)
                                     tCAATGACTGGCAGCAACACTGC‑GCGCAGCTGCGTGATGAACATTCCTGGCGTTACGACCATTCCCGGTGACGCTATCTACGCGCCGTTGTTGTTAA‑aac                                                               <  2:471438/100‑1 (MQ=255)
                                          gACTGGCAGCAACACTGCAGCGCAGCTGCGTGATGAACATTCCTGGCGTTACGACCA‑TCCCGGTGACGCTATCTACGCGCCGTTGTTGTTAA‑AACAACTg                                                          <  2:2519432/100‑1 (MQ=255)
                                          gACTGGCAGCAACACTGC‑GCGCAGCTGCGTGATGAACATTCCTGGCGTTACGACCATTCCCGGTGACGCTATCTACGCGCCGTTGTTGTTAA‑AACAACTg                                                          <  2:884884/100‑1 (MQ=255)
                                           aCTGGCAGCAACACTGC‑GCGCAGCTGCGTGATGAACATTCCTGGCGTTACGACCATTCCCGGTGACGCTATCTACGCGCCGTTGTTGTTAA‑AACAACTGt                                                         >  2:1955562/1‑100 (MQ=255)
                                           aCTGGCAGCAACACTGC‑GCGCAGCTGCGTGATGAACATTCCTGGCGTTACGACCATTCCCGGTGACGCTATCTACGCGCCGTTGTTGTTAA‑AACAACTGt                                                         >  2:182715/1‑100 (MQ=255)
                                            cTGGCAGCAACACTGC‑GCGCAGCTGCGTGATGAACATTCCTGGCGTTACGACCATTCCCGGTGACGCTATCTACGCGCCGTTGTTGTTAA‑AACAACTGTc                                                        >  1:314876/1‑100 (MQ=255)
                                            cTGGCAGCAACACTGC‑GCGCAGCTGCGGGATGAACATTCCTGGCGTTACGACCA‑TCCCGGTGACGCTATCTACGCGCCGTTGTT‑TTaacaacaacggcgc                                                       <  2:601941/100‑6 (MQ=255)
                                             tGGCAGCAACACTGC‑GCGCAGCTGCGTGATGAACATTCCTGGCGTTACGACCATTCCCGGTGACGCTATCTACGCGCCGTTGTTGTTAA‑AACAACTGTCg                                                       >  1:2562968/1‑100 (MQ=255)
                                             tGGCAGCAACACTGC‑GCGCAGCTGCGTGATGAACATTCCTGGCGTTACGACCATTCCCGGTGACGCTATCTACGCGCCGTTGTTGTTAA‑AACAACTGTCg                                                       <  1:1095453/100‑1 (MQ=255)
                                             tGGCAGCAACACTGC‑GCGCAGCTGCGTGATGAACATTCCTGGCGTTACGACCATTCCCGGTGACGCTATCTACGCGCCGTTGTTGTTAA‑AACAACTGTCg                                                       <  1:2611913/100‑1 (MQ=255)
                                              ggCAGCAACACTGC‑GCGCAGCTGCGTGATGAACATTCCTGGCGTTACGACCATTCCCGGTGACGCTATCTACGCGCCGTTGTTGTTAA‑AACAACTGTCgg                                                      >  1:782070/1‑100 (MQ=255)
                                              ggCAGCAACACTGC‑GCGCAGCTGCGTGATGAACATTCCTGGCGTTACGACCATTCCCGGTGACGCTATCTACGCGCCGTTGTTGTTAA‑AACAACTGTCgg                                                      <  1:503423/100‑1 (MQ=255)
                                               gcagcaACACTGC‑GCGCAGCTGCGTGATGAACATTCCTGGCGTTACGACCATTCCCGGTGACGCTATCTACGCGCCGTTGTTGTTAA‑AACAACTGTCGGa                                                     >  2:2228889/1‑100 (MQ=255)
                                                cagcaACACTGC‑GCGCAGCTGCGTGATGAACATTCCTGGCGTTACGACCATTCCCGGTGACGCTATCTACGCGCCGTTGTTGTTAA‑AACAACTGTCGGAt                                                    >  1:2747491/1‑100 (MQ=255)
                                                cagcaACACTGC‑GCGCAGCTGCGTGATGAACATTCCTGGCGTTACGACCATTCCCGGTGACGCTATCTACGCGCCGTTGTTGTTAA‑AACAACTGTCGGAt                                                    >  2:2771551/1‑100 (MQ=255)
                                                cagcaACACTGC‑GCGCAGCTGCGTGATGAACATTCCTGGCGTTACGACCATTCCCGGTGACGCTATCTACGCGCCGTTGTTGTTAA‑AACAACTGTCGGAt                                                    >  2:2839419/1‑100 (MQ=255)
                                                cagcaACACTGC‑GCGCAGCTGCGTGATGAACATTCCTGGCGTTACGACCATTCCCGGTGACGCTATCTACGCGCCGTTGTTGTTAA‑AACAACTGTCGGAt                                                    <  2:3007185/100‑1 (MQ=255)
                                                 agcaACACTGC‑GCGCAGCTGCGTGATGAACATTCCTGGCGTTACGACCATTCCCGGTGACGCTATCTACGCGCCGTTGTTGTTAA‑AACAACTGTCGGATc                                                   <  2:2178753/100‑1 (MQ=255)
                                                  gcaACACTGC‑GCGCAGCTGCGTGATGAACATTCCTGGCGTTACGACCATTCCCGGTGACGCTATCTACGCGCCGTTGTTGTTAA‑AACAACTGTCGGATCg                                                  <  2:505336/100‑1 (MQ=255)
                                                   caACACTGC‑GCGCAGCTGCGTGATGAACATTCCTGGCGTTACGACCATTCCCGGTGACGCTATCTACGCGCCGTTGTTGTTAA‑AACAACTGTCGGATCGt                                                 <  2:1417679/100‑1 (MQ=255)
                                                    aaCACTGC‑GCGCAGCTGCGTGATGAACATTCCTGGCGTTACGACCATTCCCGGTGACGCTATCTACGCGCCGTTGTTGTTAA‑AACAACTGTCGGATCGTa                                                <  1:2215056/100‑1 (MQ=255)
                                                        cTGC‑GCGCAGCTGCGTGATGAACATTCCTGGCGTTACGACCATTCCCGGTGACGCTATCTACGCGCCGTTGTTGTTAA‑AACAACTGTCGGATCGTAAAcc                                            <  2:375978/100‑1 (MQ=255)
                                                        cTGC‑GCGCAGCTGCGTGATGAACATTCCTGGCGTTACGACCATTCCCGGTGACGCTATCTACGCGCCGTTGTTGTTAA‑AACAACTGTCGGATCGTAAAcc                                            >  2:1732737/1‑100 (MQ=255)
                                                          gc‑gcgcAGCTGCGTGATGAACATTCCTGGCGTTACGACCATTCCCGGTGACGCTATCTACGCGCCGTTGTTGTTAA‑AACAACTGTCGGATCGTAAACCTg                                          >  2:928611/1‑100 (MQ=255)
                                                           c‑gcgcAGCTGCGTGATGAACATTCCTGGCGTTACGACCATTCCCGGTGACGCTATCTACGCGCCGTTGTTGTTAA‑GACAACTGTCGGATCGTAAACCTGc                                         <  1:2994238/100‑1 (MQ=255)
                                                           c‑gcgcAGCTGCGTGATGAACATTCCTGGCGTTACGACCATTCCCGGTGACGCTATCTACGCGCCGTTGTTGTTAA‑AACAACTGTCGGATCGTAAACCTGc                                         >  1:1483036/1‑100 (MQ=255)
                                                              cgcAGCTGCGTGATGAACATTCCTGGCGTTACGACCATTCCCGGTGACGCTATCTACGCGCCGTTGTTGTTAA‑AACAACTGTCGGATCGTAAACCTGCgg                                       <  2:845161/100‑1 (MQ=255)
                                                                  gCTGCGTGATGAACATTCCTGGCGTTACGACCATTCCCGGTGACGCTATCTACGCGCCGTTGTTGTTAA‑AACAACTGTCGGATCGTAAACCTGCGGATTg                                   <  1:2474581/100‑1 (MQ=255)
                                                                    tGCGTGATGAACATTCCTGGCGTTACGACCATTCCCGGTGACGCTATCTACGCGCCGTTGTTGTTAA‑AACAACTGTCGGATCGTAAACCTGCGGATTGcg                                 >  2:3022744/1‑100 (MQ=255)
                                                                    tGCGTGATGAACATTCCTGGCGTTACGACCATTCCCGGTGACGCTATCTACGCGCCGTTGTTGTTAA‑AACAACTGTCGGATCGTAAACCTGCGGATTGcg                                 >  1:572560/1‑100 (MQ=255)
                                                                     gCGTGATGAACATTCCTGGCGTTACGACCATTCCCGGTGACGCTATCTACGCGCCGTTGTTGTTAA‑AACAACTGTCGGATCGTAAACCTGCGGATTGcgt                                >  1:278661/1‑100 (MQ=255)
                                                                         gatgaACATTCCTGGCGTTACGACCATTCCCGGTGACGCTATCTACGCGCCGTTGTTGTTAA‑AACAACTGTCGGATCGTAAACCTGCGGATTGCGTCGTg                            <  1:1608646/100‑1 (MQ=255)
                                                                           tgaACATTCCTGGCGTTACGACCATTCCCGGTGACGCTATCTACGCGCCGTTGTTGTTAA‑AACAACTGTCGGATCGTAAACCTGCGGATTGCGTCGTGAc                          <  2:931631/100‑1 (MQ=255)
                                                                           tgaACATTCCTGGCGTTACGACCATTCCCGGTGACGCTATCTACGCGCCGTTGTTGTTAA‑AACAACTGTCGGATCGTAAACCTGCGGATTGCGTCGTGAc                          >  1:102218/1‑100 (MQ=255)
                                                                           tgaACATTCCTGGCGTTACGACCATTCCCGGTGACGCTATCTACGCGCCGTTGTTGTTAA‑AACAACTGTCGGATCGTAAACCTGCGGATTGCGTCGTGAc                          <  1:2839446/100‑1 (MQ=255)
                                                                            gaACATTCCTGGCGTTACGACCATTCCCGGTGACGCTATCTACGCGCCGTTGTTGTTAA‑AACAACTGTCGGATCGTAAACCTGCGGATTGCGTCGTGAcc                         >  1:1261131/1‑100 (MQ=255)
                                                                            gaACATTCCTGGCGTTACGACCATTCCCGGTGACGCTATCTACGCGCCGTTGTTGTTAA‑AACAACTGTCGGATCGTAAACCTGCGGATTGCGTCGTGAcc                         <  2:436705/100‑1 (MQ=255)
                                                                             aaCATTCCTGGCGTTACGACCATTCCCGGTGACGCTATCTACGCGCCGTTGTTGTTAA‑AACAACTGTCGGATCGTAAACCTGCGGATTGCGTCGTGACca                        >  1:1888899/1‑100 (MQ=255)
                                                                              aCATTCCTGGCGTTACGACCATTCCCGGTGACGCTATCTACGCGCCGTTGTTGTTAA‑AACAACTGTCGGATCGTAAACCTGCGGATTGCGTCGTGACcac                       <  1:2845531/100‑1 (MQ=255)
                                                                               cATTCCTGGCGTTACGACCATTCCCGGTGACGCTATCTACGCGCCGTTGTTGTTAA‑AACAACTGTCGGATCGTAAACCTGCGGATTGCGTCGTGACcaca                      >  2:2899862/1‑100 (MQ=255)
                                                                                aTTCCTGGCGTTACGACCATTCCCGGTGACGCTATCTACGCGCCGTTGTTGTTAA‑AACAACTGTCGGATCGTAAACCTGCGGATTGCGTCGTGACCACAg                     <  2:1835835/100‑1 (MQ=255)
                                                                                aTTCCTGGCGTTACGACCATTCCCGGTGACGCTATCTACGCGCCGTTGTTGTTAA‑AACAACTGTCGGATCGTAAACCTGCGGATTGCGTCGTGACCACAg                     <  2:1524979/100‑1 (MQ=255)
                                                                                aTTCCTGGCGTTACGACCATTCCCGGTGACGCTATCTACGCGCCGTTGTTGTTAA‑AACAACTGTCGGATCGTAAACCTGCGGATTGCGTCGTGACCACAg                     >  1:1209095/1‑100 (MQ=255)
                                                                                   ccTGGCGTTACGACCATTCCCGGTGACGCTATCTACGCGCCGTTGTTGTTAA‑AACAACTGTCGGATCGTAAACCTGCGGATTGCGTCGTGACCACAGAtg                  <  2:151903/100‑1 (MQ=255)
                                                                                    cTGGCGTTACGACCATTCCCGGTGACGCTATCTACGCGCCGTTGTTGTTAA‑AACAACTGTCGGATCGTAAACCTGCGGATTGCGTCGTGACCACAGAtgt                 >  1:2934672/1‑100 (MQ=255)
                                                                                    cTGGCGTTACGACCATTCCCGGTGACGCTATCTACGCGCCGTTGTTGTTAA‑AACAACTGTCGGATCGTAAACCTGCGGATTGCGTCGTGACCACAGAtgt                 >  1:665235/1‑100 (MQ=255)
                                                                                    cTGGCGTTACGACCATTCCCGGTGACGCTATCTACGCGCCGTTGTTGTTAA‑AACAACTGTCGGATCGTAAACCTGCGGATTGCGTCGTGACCACAGAtgt                 >  1:2477540/1‑100 (MQ=255)
                                                                                      ggCGTTACGACCATTCCCGGTGACGCTATCTACGCGCCGTTGTTGTTAA‑AACAACTGTCGGATCGTAAACCTGCGGATTGCGTCGTGACCACAGATGTgg               >  2:1353365/1‑100 (MQ=255)
                                                                                      ggCGTTACGACCATTCCCGGTGACGCTATCTACGCGCCGTTGTTGTTAA‑AACAACTGTCGGATCGTAAACCTGCGGATTGCGTCGTGACCACAGATGTgg               >  2:824546/1‑100 (MQ=255)
                                                                                       gCGTTACGACCATTCCCGGTGACGCTATCTACGCGCCGTTGTTGTTAA‑AACAACTGTCGGATCGTAAACCTGCGGATTGCGTCGTGACCACAGATGTggg              <  1:995628/100‑1 (MQ=255)
                                                                                       gCGTTACGACCATTCCCGGTGACGCTATCTACGCGCCGTTGTTCTTAA‑AACAACTGTCGGATCGTAAACCTGCGGATTGCGTCGTGACCACAGATGTggg              <  2:1971241/100‑1 (MQ=255)
                                                                                        cGTTACGACCATTCCCGGTGACGCTATCTACGCGCCGTTGTTGTTAA‑AACAACTGTCGGATCGTAAACCTGCGGATTGCGTCGTGACCACAGATGTgggg             >  1:1380502/1‑100 (MQ=255)
                                                                                        cGTTACGACCATTCCCGGTGACGCTATCTACGCGCCGTTGTTGCTAA‑AACAACTGTCGGATCGTAAACCTGCGGATTGCGTCGTGACCACAGATGTgggg             <  2:1967797/100‑1 (MQ=255)
                                                                                         gTTACGACCATTCCCGGTGACGCTATCTACGCGCCGTTGTTGTTAA‑AACAACTGTCGGATCGTAAACCTGCGGATTGCGTCGTGACCACAGATGTGGGgc            >  1:1235240/1‑100 (MQ=255)
                                                                                         gTTACGACCATTCCCGGTGACGCTATCTACGCGCCGTTGTTGTTAA‑AACAACTGTCGGATCGTAAACCTGCGGATTGCGTCGTGACCACAGATGTGGGgc            <  1:362131/100‑1 (MQ=255)
                                                                                            aCGACCATTCCCGGTGACGCTATCTACGCGCCGTTGTTGTTAA‑AACAACTGTCGGATCGTAAACCTGCGGATTGCGTCGTGACCACAGATGTGGGgcagc         <  1:2690609/100‑1 (MQ=255)
                                                                                             cGACCATTCCCGGTGACGCTATCTACGCGCCGTTGTTGTTAA‑AACAACTGTCGGATCGTAAACCTGCGGATTGCGTCGTGACCACAGATGTGGGgcagca        <  1:2635059/100‑1 (MQ=255)
                                                                                             cGACCATTCCCGGTGACGCTATCTACGCGCCGTTGTTGTTAA‑AACAACTGTCGGATCGTAAACCTGCGGATTGCGTCGTGACCACAGATGTGGGgcagca        >  1:1911044/1‑100 (MQ=255)
                                                                                             cGACCATTCCCGGTGACGCTATCTACGCGCCGTTGTTGTTAA‑AACAACTGTCGGATCGTAAACCTGCGGATTGCGTCGTGACCACAGATGTGGGgcagca        >  1:2413191/1‑100 (MQ=255)
                                                                                                  a‑TCCCGGTGACGCTATCTACGCGCCGTTGTTGTTAA‑AACAACTGTCGGATCGTAAACCTGCGGATTGCGTCGTGACCACAGATGTGGGGCAGCACCAGAt  <  2:1146401/100‑1 (MQ=255)
                                                                                                   |                                                                                                    
TAAATGCTCTGTTACCAGCATTACAGCAGCCGTTAAATCAATGACTGGCAGCAACACTGC‑GCGCAGCTGCGTGATGAACATTCCTGGCGTTACGACCA‑TCCCGGTGACGCTATCTACGCGCCGTTGTTGTTAA‑AACAACTGTCGGATCGTAAACCTGCGGATTGCGTCGTGACCACAGATGTGGGGCAGCACCAGAT  >  NC_000913/3951500‑3951696

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A