Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A3 F228 I0 R1	182	117.2	5818924	93.6%	5446512	99.8

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_000913	4,128,359	C→T	74.2%	A13T (GCT→ACT)	metJ ←	transcriptional repressor, S‑adenosylmethionine‑binding

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,128,359	0	C	T	74.2%	192.5 / 74.9	123	A13T (GCT→ACT)	metJ	transcriptional repressor, S‑adenosylmethionine‑binding
Reads supporting (aligned to +/- strand): ref base C (16/16); new base T (39/52); total (55/68)
Fisher's exact test for biased strand distribution p-value = 5.38e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 124 total aligned reads displayed.

CTGACGACGCGTGCGTTCATCGGTGAGGATTTTTAACACCTTAAGAGGAATGGAAACCGTAATCTTTTTGACTTGTTCACTCTTCTTGCCGTGCTCAGCGTATGGGCTGATATATTCGCCGCTCCATTCAGCCATGAGATACTTAATCCTCTTCGTCAATAAATTGAGACCAGACCACACAGTTGATGTGGGTAC  >  NC_000913/4128261‑4128455

cTGACTACCCGTTCTTTCAGCTGTTATTATTTTTAACACCTTAAGAGGAATGGAAACCGTAATCTTTTTGACTTGTTCACTCCTCTTGCCGTGCTCAGCg                                                                                                 <  2:1694147/100‑1 (MQ=255)

 tGACGACGCGTGCGTTCATCGGTGAGGATTTTTAACACCTTAAGAGGAATGGAAACCGTAATCTTTTTGACTTGTTCACTCTTCTTGCCGTGCTCAgtgt                                                                                                >  1:1260273/1‑100 (MQ=255)

    cgacgCTCGCGTTCATCGGTGAGGATTTTTAACACCTTAAGAGGAATGGAAACCGTAATCTTTTTGACTTGTTCACTCTTCTTGCCGTGCTCAGTGTATg                                                                                             <  2:1736086/100‑1 (MQ=255)

      acgCTTGCGTTCATCGGTGATGATTTTTAACACCTTAAGAGGAATGGAAACCGTAATCTTTTTGACTTGTTCACTCTTCTTGCCGTGCTCAGCGTATgg                                                                                            <  1:377250/99‑1 (MQ=255)

        gcgtgcgtTCATCGGTGAGGATTTTTAACACCTTAAGAGGAATGGAAACCGTAATCTTTTTGACTTGTTCACTCTTCTTGCCGTGCTCAGTGTATGGGCt                                                                                         >  2:2206668/1‑100 (MQ=255)

           tgcgtTCATCGGTGAGGATTATTAACACCTTAAGAGGAATGGAAACCGTAATCTTTTTGACTTGTTCACTCTTCTTGCCGTGCTCAGTGTATGGGCTGat                                                                                      >  1:2579346/1‑100 (MQ=255)

            gctttCATCGGTGAGGATTTTTAACACCTTAAGAGGAATGGAAACCGTAATCTTTTTGACTTGTTCACTCTTCTTGCCGAGCTCAGCGTATGGGCTGata                                                                                     <  1:538609/97‑1 (MQ=255)

            gcgtTCATCGGTGAGGATTTTTAACACCTTAAGAGGAATGGAAACCGTAATCTTTTTGACTTGTTCACTCTTCTTGCCGTGCTCAGTGTATGGGCTGata                                                                                     <  2:1174003/100‑1 (MQ=255)

            gcgtTCATCGGTGAGGATTTTTAACACCTTAAGAGGAATGGAAACCGTAATCTTTTTGACTTGTTCACTCTTCTTGCCGTGCTCAGTGTATGGGCTGata                                                                                     <  1:1348785/100‑1 (MQ=255)

             cgtTCATCGGCGAGGATTTTTAACACCTTAAGAGGAATGGAAACCGTAATCTTTTTGACTTGTTCACTCTTCTTGCCGTGCTCAGCGTATGGGCTGatat                                                                                    >  2:1446716/1‑100 (MQ=255)

              gtTCAGCGGTGAGGATTTTTAACACCTTAAGAGGAATGGAAACCGTAATCTTTTTGACTTGTTCACTCTTCTTGCCGTGCTCAGTGTATGGGCTGatata                                                                                   <  1:951287/100‑1 (MQ=255)

                tCATCGGTGAGGATTTTTAACACCTTAAGAGGAATGGAAACCGTAATCTTTTTGACTTGTTCACTCTTCTTGCCGTGCTCAGTGTATGGGCTGatatat                                                                                  <  1:1682363/99‑1 (MQ=255)

                  aTCGGTGAGGATTTTTAACACTTTAAGCGGAATAGAAACCGTAATCTTTTTGCCTTGTGCACTCTTCTTGCCTTGCTCAGTTTATGGGCTTATATATTcg                                                                               >  1:767651/1‑100 (MQ=255)

                  aTCGGTGAGGATTTTTAACACCTTAAGAGGAATGGAAACCGTAATCTTTTTGACTTGTTCACTCTTCTTGCCGTGCTCAGTGTATGGGCTGATATATTcg                                                                               >  2:2570122/1‑100 (MQ=255)

                    cGGTGAGGATTTTTAACACCATAAAAGTAATGCAAACCGTAATCTTTTTGACTTGTCCACTTTTCTTTCTGAGCTCATCGTATTGGCTGttatat                                                                                  >  1:2161256/1‑95 (MQ=255)

                     ggTGAGGATTTTTAACACCTTAAGAGGAATGGAAACCGTAATCTTTTTGACTTGTTCACTCTTCTTGCCGTGCTCAGTGTATGGGCTGATATATTcgccg                                                                            >  2:1153818/1‑100 (MQ=255)

                      gTGAGGATTTTTAACACCTTAAGAGGAATGGAAACCGTAATCTTTTTGACTTGTTCACTCTTCTTGCCGTGCTCAGTGTATGGGCTGATATATTcgccgc                                                                           <  2:2796391/100‑1 (MQ=255)

                       tGAGGATTTTTAACACCTTAAGAGGAATGGAAACCGTAATCTTTTTGACTTGTTCACTCTTCTTGCCGTGCTCAGTGTATGGGCTGATATATTCGCCGCt                                                                          >  2:1046951/1‑100 (MQ=255)

                       tGAGGATTTTTAACACCTTAAGAGGAATGGAAACCGTAATCTTTTTGACTTGTTCACTCTTCTTGCCGTGCTCAGTGTATGGGCTGATATATTCGCCGCt                                                                          <  1:550160/100‑1 (MQ=255)

                       tGAGGATTTTTAACACCTTAAGAGGAATGGAAACCGTAATCTTTTTGACTTGTTCACTCTTCTTGCCGTGCTCAGTGTATGGGCTGATATATTCGCCGCt                                                                          >  2:2087566/1‑100 (MQ=255)

                       cgAGGATTTTTAACACCTTAAGAGGAATGGAAACCGTAATCTTTTTGACTTGTTCACTCTTCTTGCCGTGCTCAGCGTATGGGCTGATATATTCGCCGCt                                                                          <  2:177240/99‑1 (MQ=255)

                        gAGGATTTTTAACACCTTAAGAGGAATGGAAACCGTAATCTTTTTGACTTGTTCACTCTTCTTGCCGTGCTCAGTGTATGGGCTGATATATTCGCCGCt                                                                          >  1:155423/1‑99 (MQ=255)

                         aGGATTTTTAACACCTTAAGAGGAATGGAAACCGTAATCTTTTTGACTTGTTCACTCTTCTTGCCGTGCTCAGTGTATGGGCTGATATATTCGCCGCTcc                                                                        <  2:1854360/100‑1 (MQ=255)

                          ggATTTTTAACACCTTAAGAGGAATGGAAACCGTAATCTTTTTTTCTTTTTTATTCTTCTTGCCGTGCTCAGTGTATGGTATGATCTATTCGCCGATcta                                                                       >  1:200899/1‑98 (MQ=255)

                          ggATTTTTAACACCTTAAGAGGAATGGAAACCGTAATCTTTTTGACTTGTTGACTCTTGTTGCCGTGCTCAGTGTATGGGCTGATATATTCGCCGCTcc                                                                        >  1:356868/1‑99 (MQ=255)

                          ggATTTTTAACACCTTAAGAGGAATGGAAACCGTAATCTTTTTGACTTGTTCACTCTTCTTGCCGTGCTCAGTGTATGGGCTGATATATTCGCCGCTCCa                                                                       >  2:2659160/1‑100 (MQ=255)

                          ggATTTTTAACACCTTAAGAGGAATGGAAACCGTAATCTTTTTGACTTGTTCACTCTTCTTGCCGTGCTCAGTGTATGGGCTGATATATTCGCCGCTCCa                                                                       >  2:2598085/1‑100 (MQ=255)

                           gATTTTTAACACCTTAAGAGGAATGGAAACCGTAATCTTTTTGACTTGTTCACTCTTCTTGCCGTGCTCAGTGTATGGGCTGATATATTCGCCGCTCCAt                                                                      <  1:1153816/100‑1 (MQ=255)

                           gATTTTTAACACCTTAAGAGGAATGGAAACCGTAATCTTTTTGACTTGTTCACTCTTCTTGCCGTGCTCAGTGTATGGGCTGATATATTCGCCGCTCCAt                                                                      >  1:1620231/1‑100 (MQ=255)

                            aTTTTTAACACCTTAAGAGGAATGGAAACCGTAATCTTTTTGACTTGTTCACTCTTCTTGCCGTGCTCAGCGTATGGGCTGATATATTCGCCGCTCCAtt                                                                     >  1:749773/1‑100 (MQ=255)

                            aTTTTTAACACCTTAAGAGGAATGGAAACCGTAATCTTTTTGACTTGTTCACTCTTCTTGCCGTGCTCAGCGTATGGGCTGATATATTCGCCGCTCCAtt                                                                     >  1:408580/1‑100 (MQ=255)

                              ttttAACACCTTAAGAGGAATGGAAACCGTAATCTTTTTGACTTGTTCACTCTTCTTGCCGTGCTCAGTGTATGGGCTGATATATTCGCCGCTCCATTCa                                                                   <  2:46824/100‑1 (MQ=255)

                               tttCACCCCTTAAGAGGTATGGAAACCGTAATCTTTTTGACTTGTTCACTCTTCTTGCCGTGCTCAGTGTGTGGGCTGATATATTCGCCGCTCCATTCAg                                                                  <  2:2611058/100‑1 (MQ=255)

                               tttAACACCTTAAGAGGAATGGAAACCGTAATCTTTTTGACTTGTTCACTCTTCTTGCCGTGCTCAGTGTATGGGCTGATATATTCGCCGCTCCATTCAg                                                                  >  2:1315116/1‑100 (MQ=255)

                               tttAACACCTTAAGAGGAATGGAAACCGTAATCTTTTTGACTTGTTCACTCTTCTTGCCGTGCTCAGTGTATGGGCTGATATATTCGCCGCTCCATTCAg                                                                  <  2:2172471/100‑1 (MQ=255)

                               tttAACACCTTAAGAGGAATGGAAACCGTAATCTTTTTGACTTGTTCACTCCTCTTGCCGTGCTCAGCGTATGGGCTGATATATTCGCCGCTCCATTCAg                                                                  >  1:1865788/1‑100 (MQ=255)

                                 tAACACCTTAAGAGGAATGGAAACCGTAATCTTTTTGACTTGTTCACTCTTCTTGCCGTGCTCAGTGTATGGGCTGATATATTCGCCGCTCCATTCAGcc                                                                <  2:708623/100‑1 (MQ=255)

                                  aaCACCTTAAGAGGAATGGAAACCGTAATCTTTTTTTTTTTTTTACTCTTCTTGCCGTGCTCAGTGTATGGGCTGATATATTCGCCGCTCCATTCAGc                                                                 >  1:50251/1‑98 (MQ=255)

                                    cacCTTAAGAGGAATGGAAACCGTAATCTTTTTGACTTGTTCACTCTTCTTGCCGTGCTCAGTGTATGGGCTGATATATTCGCCGCTCCATTCAGCCATg                                                             <  1:1111686/100‑1 (MQ=255)

                                     acCTTAAGAGGAATGGAAACCGTAATCTTTTTGACTTGTTCACTCTTCTTGCCGTGCTCAGTGTATGGGCTGATATATTCGCCGCTCCATTcctcttaag                                                            >  2:2737360/1‑92 (MQ=255)

                                     acCTTAAGAGGAATGGAAACCGTAATCTTTTTGACTTGTTCACTCTTCTTGCCGTGCTCAGTGTATGGGCTGATATATTCGCCGCTCCATTCAGCCATga                                                            <  1:1478023/100‑1 (MQ=255)

                                      ccTTAAGAGGAATGGAAACCGTAATCTTTTTGACTTGTTCACTCTTCTTGCCGTGCTCAGTGTATGGGCTGATATATTCGCCGCTCCATTCAGCCATgag                                                           <  2:1596890/100‑1 (MQ=255)

                                      ccTTAAGAGGAATGGAAACCGTAATCTTTTTGACTTGTTCACTCTTCTTGCCGTGCTCAGTGTATGGGCTGATATATTCGCCGCTCCATTCAGCCATgag                                                           <  1:1345536/100‑1 (MQ=255)

                                      ccTTAAGAGGAATGGAAACCGTAATCTTTTTGACTTGTTCACTCTTCTTGCCGTGCTCAGTGTATGGGCTGATATATTCGCCGCTCCATTCAGCCATgag                                                           <  2:1208226/100‑1 (MQ=255)

                                        ttAAGAGGAATGGAAACCGTAATCTTTTTGACTTGTTCACTCCTCTTGCCGTGCTCAGCGTATGGGCTGATATATTCGCCGCTCCATTCAGCCATGAGAt                                                         >  2:2148590/1‑100 (MQ=255)

                                         tAAGAGGAATGTAAACCGTAATCTTTTTGACTTGTTCACTCCTCTTGCCTTTCTCAGCGTATGGGCTGTTATATTCGCCGCTCCATTCAGCCATGAGAt                                                         <  1:1000313/99‑1 (MQ=255)

                                            gagGAATGGAAACCGTAATCTTTTTGACTTGTTCACTCTTCTTGCCGTGCTCAGTGTATGGGCTGATATATTCGCCGCTCCATTCAGCCATGAGATACtt                                                     <  2:1120500/100‑1 (MQ=255)

                                              ggAATGGAAACCGTAATCTTTTTGACTTGTTCACTCTTCTTGCCGTGCTCAGCGTATGGGCTGATATATTCGCCGCTCCATTCAGCCATGAGATACTTaa                                                   >  2:547224/1‑100 (MQ=255)

                                              ggAATGGAAACCGTAATCTTTTTGACTTGTTCACTCCTCTTGCCGTGCTCAGCGTATGGGCTGATATATTCGCCGCTCCATTCAGCCATGAGATACTTaa                                                   >  2:375841/1‑100 (MQ=255)

                                                 aTGGAAACCGTAATCTTTTTGACTTGTTCTCTCTTCTTGCCGTGCTCAGTGTATGGGCTGATATATTCGCCGCTCCATTCAGCCATGAGATACTTAATcc                                                <  1:1356862/100‑1 (MQ=255)

                                                 aTGGAAACCGTAATCTTTTTGACTTGTTCACTCTTCTTGCCGTGCTCAGTGTATGGGCTGATATATTCGCCGCTCCATTCAGCCATGAGATACTTAATcc                                                <  2:783459/100‑1 (MQ=255)

                                                 aTGGAAACCGTAATCTTTTTGACTTGTTCACTCTTCTTGCCGTGCTCAGTGTATGGGCTGATATATTCGCCGCTCCATTCAGCCATGAGATACTTAATcc                                                <  1:1765964/100‑1 (MQ=255)

                                                 aTGGAAACCGTAATCTTTTTGACTTGTTCACTCTTCTTGCCGTGCTCAGTGTATGGGCTGATATATTCGCCGCTCCATTCAGCCATGAGATACTTAATcc                                                <  1:2440961/100‑1 (MQ=255)

                                                 aTGGAAACCGTAATCTTTTTGACTTGTTCACTCTTCTTGCCGTGCTCAGTGTATGGGCTGATATATTCGCCGCTCCATTCAGACATGAGATACTTAATcc                                                <  1:398622/100‑1 (MQ=255)

                                                   ggAAACCGTAATCTTTTTGACTTGTTCACTCTTCTTGCCGTGCTCAGTGTATGGGCTGATATATTCGCGGCTCCATTCAGCCATGAGATACTTAATTct                                               >  1:2511278/1‑99 (MQ=255)

                                                   ggAAACCGTAATCTTTTTGACTTGTTCACTCTTCTTGCCGTGCTCAGTGTATGGGCTGATATATTCGCCGCTCCATTCAGCCATGAGATACTTAATCctc                                              >  2:322655/1‑100 (MQ=255)

                                                    gAAACCGTAATCTTTTTGACTTGTTCACTCTTCTTGCCGTGCTCAGTGTATGGGCTGATATATTCGCCGCTCCATTCAGCCATGAGATACTTAATCctct                                             >  1:529183/1‑100 (MQ=255)

                                                       aCCGTAATCTTTTTGACTTGTTCACTCTTCTTGCCGTGCTCAGTGTATGGGCTGATATATTCGCCGCTCCATTCAGCCATGAGATACTTAATACTCTTCg                                          >  1:1291508/1‑100 (MQ=255)

                                                          gTAATCTTTTTGACTTGTTCACTCTTCTTGCCGTGCTCAGTGTATGGGCTGATATATTCGCCGCTCCATTCAGCCATGAGATACTTAATCCTCTTCGTCa                                       >  1:1605873/1‑100 (MQ=255)

                                                          gTAATCTTTTTGACTTGTTCACTCTTATTGCCGTGCTCAGTGTATGGGCTGATATATTCGCCGCTCCATTCAGCCATGAGATACTTAATCCTCTTCGTCa                                       >  2:1767456/1‑100 (MQ=255)

                                                          gTAAGCTTTTTGACTTGTGCACTGTTCTGGCCGCGCTCAGTGTATGGGCTGATATATTCGCCGCTCCATTCAGCCATGAGATACTTAATCCTCTTCGTc                                        <  1:2323469/99‑1 (MQ=255)

                                                            aaTCTTTTTGACTTGTTCACTCTTCTTGCCGAGCTCAGCGTATGGGCTGATATATTCGCCGCTCCATTCAGCCATGCGATACTTAATCCTCTTCGTCAAt                                     >  2:2898439/1‑100 (MQ=255)

                                                             aTCTTTTTGACTTGTTCACTCTTCTTGCCGTGCTCAGTGTATGGGCTGATATATTCGCCGCTCCATTCAGCCATGAGATACTTAATCCTCTTCGTCAAt                                     <  2:2533460/99‑1 (MQ=255)

                                                             aTCTTTTTGACTTGTTCACTCTTCTTGCCGTGCTCAGTGTATGGGCTGATATATTCGCCGCTCCATTCAGCCATGAGATACTTAATCCTCTTCGTCAATa                                    <  1:1185769/100‑1 (MQ=255)

                                                              tCTTTTTGACTTGTTCACTCTTCTTGCCGTGCTCAGTGTATGGGCTGATATATTCGCCGCTCCATTCAGCCATGAGATACTTAATCCTCTTCGTCAATaa                                   <  2:681710/100‑1 (MQ=255)

                                                                 ttttGACTTGTTCACTCTTCTTGCCGTGCTCAGTGTATGGGCTGATATATTCGCCGCTCCATTCAGCCATGAGATACTTAATCCTCTTCGTCAATAAAtt                                <  2:2807953/100‑1 (MQ=255)

                                                                 ttttGACTTGTTCACTCTTCTTGCCGTGCTCAGCGTATGGGCTGATATATTCGCCGCTCCATTCAGCCATGAGATACTTAATCCTCTTCGTCAATAAAtt                                <  1:2838162/100‑1 (MQ=255)

                                                                 ttttGACTTGTTCACTCTTCTTGCCGTGCTCAGCGTATGGGCTGATATATTCGCCGCTCCATTCAGCCATGAGATACTTAATCCTCTTCGTCAATAAAtt                                <  1:2838486/100‑1 (MQ=255)

                                                                  tttGACTTGTTCACTCTTCTTGTCGTGCTCACTGTATTGGCTGATATTTTCGCCGCTCCATTCAGCCATGAGATACTTAATCCTCTTCGTCAATAAATTg                               <  1:1961407/100‑1 (MQ=255)

                                                                  tttGACTTGTTCACTCTTCTTGCCGTGCTCAGTGTATGGGCTGATATATTCGCCGCTCCATTCAGCCATGAGATACTTAATCCTCTTCGTCAATAAATTg                               <  2:772510/100‑1 (MQ=255)

                                                                  tttGACTTGTTCACTCTTCTTGCCGTGCTCAGTGTATGGGCTGATATATTCGCCGCTCCATTCAGCCATGAGATACTTAATCCTCTTCGTCAATAAATTg                               <  2:2701276/100‑1 (MQ=255)

                                                                  tttGACTTGTTCACTCTTCTTGCCGTGCTCAGCGTATGGGCTGATATATTCGCCGCTCCATTCAGCCATGAGATACTTAATCCTCTTCGTCAATAAATTg                               <  1:510487/100‑1 (MQ=255)

                                                                    tGACTTGTTCACTCTTCTTGCCGTGCTCAGTGTATGGGCTGATATATTCGCCGCTCCATTCAGCCATGAGATACTTAATCCTCTTCGTCAATNAANTgag                             <  1:369358/100‑1 (MQ=255)

                                                                    tGACTTGTTCACTCTTCTTGCCGTGCTCAGTGTATGGGCTGATATATTCGCCGCTCCATTCAGCCATGAGATACTTAATCCTCTTCGTCAATAAATTgag                             <  1:108032/100‑1 (MQ=255)

                                                                    tGACTTGTTCACTCTTCTTGCCGTGCTCAGTGTATGGGCTGATATATTCGCCGCTCCATTCAGCCATGAGATACTTAATCCTCTTCGTCAATAAATTgag                             >  1:2519555/1‑100 (MQ=255)

                                                                     gACTTGTTCACTCTTCTTGCCGTGCTCAGTGTATGGGCTGATATATTCGCCGCTCCATTCAGCCATGAGATACTTAATCCTCTTCGTCAATAAATTgaga                            >  2:2456255/1‑100 (MQ=255)

                                                                     gACTTGTTCACTCTTCTTGCCGTGCTCAGTGTATGGGCTGATATATTCGCCGCTCCATTCAGCCATGAGATACTTAATCCTCTTCGTCAATAAATTgaga                            <  1:429686/100‑1 (MQ=255)

                                                                     gACTTGTTCACTCCTCTTGCCGTGCTCAGCGTATGGGCTGATATATTCGCCGCTCCATTCAGCCATGAGATACTTAATCCTCTTCGTCAATAAATTgaga                            >  2:1471917/1‑100 (MQ=255)

                                                                      aCTTGTTCACTCTTCTTGCCGTGCTCAGTGTATGGGCTGATATATTCGCCGCTCCATTCAGCCATGAGATACTTAATCCTCTTCGTCAATAAATTgaga                            <  1:1841910/99‑1 (MQ=255)

                                                                       cTTGTTCACTCTTCTTGCCGTGCTCAGCGTATGGGCTGATATATTCGCCGCTCCATTCAGCCATGAGATACTTAATCCTCTTCGTCAATAAATTGagacc                          <  2:377252/100‑1 (MQ=255)

                                                                          gTTCACTCTTCTTGCCGTGCTCAGTGTATGGGCTGATATATTCGCCGCTCCATTCAGCCATGAGATACTTAATCCTCTTCGTCAATAAATTGagaccaga                       >  2:1738725/1‑100 (MQ=255)

                                                                          gTTCACTCTTCTTGCCGTGCTCAGTGTATGGGCTGATATATTCGCCGCTCCATTCAGCCATGAGATACTTAATCCTCTTCGTCAATAAATTGagaccaga                       <  2:2729196/100‑1 (MQ=255)

                                                                            tCACTCTTCTTGCCGTGCTCAGTGTATGGGCTGATATATTCGCCGCTCCATTCAGCCATGAGATACTTAATCCTCTTCGTCAATAAATTGagaccagacc                     <  2:603308/100‑1 (MQ=255)

                                                                            tCACTCTTCTTGCCGTGCTCAGTGTATGGGCTGATATATTCGCCGCTCCATTCAGCCATGAGATACTTAATCCTCTTCGTCAATAAATTGagaccagacc                     <  2:603576/100‑1 (MQ=255)

                                                                            tCACTCCTCTTGCCGTGCTCAGCGTATGGGCTGATATATTCGCCGCTCCATTCAGCCATGAGATACTTAATCCTCTTCGTCAATAAATTGagaccagacc                     <  1:171476/100‑1 (MQ=255)

                                                                             cACTCTTCTTGCCGTGCTCAGTGTATGGGCTGATATATTCGCCGCTCCATTCAGCCATGAGATACTTAATCCTCTTCGTCAATAAATTGAgaccagacca                    >  2:1875535/1‑100 (MQ=255)

                                                                             cACTCTTCTTGCCGTGCTCAGTGTATGGGCTGATATATTCGCCGCTCCATTCAGCCATGAGATACTTAATCCTCTTCGTCAATAAATTGAgaccagacca                    >  2:2148251/1‑100 (MQ=255)

                                                                             cACTCTTCTTGCCGTGCTCAGTGTATGGGCTGATATATTCGCCGCTCCATTCAGCCATGAGATACTTAATCCTCTTCGTCAATAAATTGAgaccagacca                    >  2:1554197/1‑100 (MQ=255)

                                                                             cACTCCTCTTGCCGTGCTCAGCGTATGGGCTGATATATTCGCCGCTCCATTCAGCCATGAGATACTTAATCCTCTTCGTCAATAAATTGAgaccagacca                    >  2:2476646/1‑100 (MQ=255)

                                                                              aCTCTTCTTGCCGTGCTCAGTGTATGGGCTGATATATTCGCCGCTCCATTCAGCCATGAGATACTTAATCCTCTTCGTCAATAAATTGAGACCAGACcac                   <  2:2656812/100‑1 (MQ=255)

                                                                               ctctTCTTGCCGTGCTCAGTGTATGGGCTGATATATTCGCCGCTCCATTCAGCCATGAGATACTTAATCCTCTTCGTCAATAAATTGAGACCAGACcaca                  >  1:313272/1‑100 (MQ=255)

                                                                                    cttGCCGTGCTCAGTGTATGGGCTGATATATTCGCCGCTCCATTCAGCCATGAGATACTTAATCCTCTTCGTCAATAAATTGAGACCAGACCACACAGtt             <  2:2089205/100‑1 (MQ=255)

                                                                                    cttGCCGTGCTCAGCGTATGGGCTGATATATTCGCCGCTCCATTCAGCCATGAGATACTTAATCCTCTTCGTCAATAAATTGAGACCAGACCACACAGtt             >  2:1036303/1‑100 (MQ=255)

                                                                                        ccGTGCTCAGTGTATGGGCTGATATATTCGCCGCTCCATTCAGCCATGAGATACTTAATCCTCTTCGTCAATAAATTGAGACCAGACCACACAGTTGAtg         <  2:1604599/100‑1 (MQ=255)

                                                                                         cGTGCTCAGTGTATGGGCTGATATATTCGCCGCTCCATTCAGCCATGAGATACTTAATCCTCTTCGTCAATAAATTGAGACCAGACCACACAGTTGAtgt        <  1:654733/100‑1 (MQ=255)

                                                                                            gCTCAGTGTATGGGCTGATATATTCGCCGCTCCATTCAGCCATGAGATACTTAATCCTCTTCGTCAATAAATTGAGACCAGACCACACAGTTGATGTggg     >  1:1077401/1‑100 (MQ=255)

                                                                                              tCAGTGTATGGGCTGATATATTCGCCGCTCCATTCAGCCATGAGATACTTAATCCTCTTCGTCAATAAATTGAGACCAGACCACACAGTTGATGTGGGTa   <  1:2570111/100‑1 (MQ=255)

                                                                                              tCAGCGTATGGGCTGATATATTCGCCGCTCCATTCAGCCATGAGATACTTAATCCTCTTCGTCAATAAATTGAGACCAGACCACACAGTTGATGTGGGTa   <  1:2886962/100‑1 (MQ=255)

                                                                                              tCAGCGTATGGGCTGATATATTCGCCGCTCCATTCAGCCATGAGATACTTAATCCTCTTCGTCAATAAATTGAGACCAGACCACACAGTTGATGTGGGTa   <  1:2205541/100‑1 (MQ=255)

                                                                                               cAGCGTATGGGCTGATATATTCGCCGCTCCATTCAGCCATGAGATACTTAATCCTCTTCGTCAATAAATTGAGACCAGACCACACAGTTGATGTGGGTAc  <  1:129049/100‑1 (MQ=255)

CTGACGACGCGTGCGTTCATCGGTGAGGATTTTTAACACCTTAAGAGGAATGGAAACCGTAATCTTTTTGACTTGTTCACTCTTCTTGCCGTGCTCAGCGTATGGGCTGATATATTCGCCGCTCCATTCAGCCATGAGATACTTAATCCTCTTCGTCAATAAATTGAGACCAGACCACACAGTTGATGTGGGTAC  >  NC_000913/4128261‑4128455

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 12 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A