Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A3 F181 I0 R1 65 126.1 6126155 96.5% 5911739 99.9

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 4,128,359 C→T 100% A13T (GCT→ACT)  metJ ← transcriptional repressor, S‑adenosylmethionine‑binding

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,128,3590CT87.6% 358.1 / 37.9 138A13T (GCT→ACT) metJtranscriptional repressor, S‑adenosylmethionine‑binding
Reads supporting (aligned to +/- strand):  ref base C (7/10);  new base T (54/66);  total (62/76)
Fisher's exact test for biased strand distribution p-value = 8.01e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 4.06e-01

Only 100 of 139 total aligned reads displayed.

CCTGACGACGCGTGCGTTCATCGGTGAGGATTTTTAACACCTTAAGAGGAATGGAAACCGTAATCTTTTTGACTTGTTCACTCTTCTTGCCGTGCTCAGCGTATGGGCTGATATATTCGCCGCTCCATTCAGCCATGAGATACTTAATCCTCTTCGTCAATAAATTGAGACCAGACCACACAGTTGATGTGGGTAC  >  NC_000913/4128260‑4128455
                                                                                                   |                                                                                                
  tGACGACGCGTGCGTTCATCGGTGAGGATTTTTAACACCTTAAGAGGAATGGAAACCGTAATCTTTTTGACTTGTTCACTCTTCTTGCCGTGCTCAGCGt                                                                                                >  1:1659491/1‑100 (MQ=255)
   gacgacGCGTGCGTTCATCGGTGAGGATTTTTAACACCTTAAGAGGAATGGAAACCGTAATCTTTTTGACTTGTTCACTCTTCTTGCCGTGCTCAGTGTa                                                                                               >  1:344045/1‑100 (MQ=255)
   gacgacGCGTGCGTTCATCGGTGAGGATTTTTAACACCTTAAGAGGAATGGAAACCGTAATCTTTTTGACTTGTTCACTCTTCTTGCCGTGCTCAGTGTa                                                                                               >  2:2972307/1‑100 (MQ=255)
   gacgacGCGTGCGTTCATCGGTGAGGATTTTTAACACCTTAAGAGGAATGGAAACCGTAATCTTTTTGACTTGTTCACTCTTCTTGCCGTGCTCAGTGTa                                                                                               >  1:1710662/1‑100 (MQ=255)
   gacgacGCGTGCGTTCATCGGTGAGGATTTTTAACACCTTAAGAGGAATGGAAACCGTAATCTTTTTGACTTGTTCACTCTTCTTGCCGTGCTCAGCGTa                                                                                               <  1:348364/100‑1 (MQ=255)
       acgCGTGCGTTCATCGGTGAGGATTTTTAACACCTTAAGAGGAATGGAAACCGTAATCTTTTTGACTTGTTCACTCTTCTTGCCGTGCTCAGTGTATgg                                                                                            >  1:1846875/1‑99 (MQ=255)
        cgcgTGCGTTCATCGGTGAGGATTTTTAACACCTTAAGAGGAATGGAAACCGTAATCTTTTTGACTTGTTCACTCTTCTTGCCGTGCTCAGTGTATGGGc                                                                                          <  1:1306810/100‑1 (MQ=255)
         gcgtgcgtTCATCGGTGAGGATTTTTAACACCTTAAGAGGAATGGAAACCGTAATCTTTTTGACTTGTTCACTCTTCTTGCCGTGCTCAGCGTATGGGCt                                                                                         <  2:428703/100‑1 (MQ=255)
          cgtgcgtTCATCGGTGAGGATTTTTAACACCTTAAGAGGAATGGAAACCGTAATCTTTTTGACTTGTTCACTCTTCTTGCCGTGCTCAGTGTATGGGCTg                                                                                        >  1:276665/1‑100 (MQ=255)
          cgtgcgtTCATCGGTGAGGATTTTTAACACCTTAAGAGGAATGGAAACCGTAATCTTTTTGACTTGTTCACTCTTCTTGCCGTGCCCAGCGTATGGGCTg                                                                                        <  1:534152/100‑1 (MQ=255)
           gtgcgtTCATCGGTGAGGATTTTTAACACCTTAAGAGGAATGGAAACCGTAATCTTTTTGACTTGTTCACTCTTCTTGCCGTGCTCAGTGTATGGGCTGa                                                                                       >  2:1433699/1‑100 (MQ=255)
             gcgtTCATCGGTGAGGATTTTTAACACCTTAAGAGGAATGGAAACCGTAATCTTTTTGACTTGTTCACTCTTCTTGCCGTGCTCAGTGTATGGGCTGat                                                                                      <  2:2230178/99‑1 (MQ=255)
             gcgtTCATCGGGGAGGATTTTTAACAGCTTGAGAGGAATGGAAACCGTAATCTGTTTGACTTGTTCACTCTTCTTGCCGTGCTCAGTGTATGGGCTGata                                                                                     <  1:799711/100‑1 (MQ=255)
                 tCATCGGTGAGGATTTTTAACACCTTAAGAGGAATGGAAACCGTAATCTTTTTGACTTGTTCACTCTTCTTGCCGTGCTCAGCGTATGGGCTGATATAtt                                                                                 >  2:660725/1‑100 (MQ=255)
                  cATCGGTGAGGATTTTTAACACCTTAAGAGGAATGGAAACGGTAATCTTTTTGACTTGTTCACTCTTATTGCCATGCTCATTGTATGGGCTAATATAtt                                                                                 >  1:1137699/1‑99 (MQ=255)
                  cATCGGTGAGGATTTTTAACACCTTAAGAGGAATGGAAACCGTAATCTTTTTGACTTGTTCACTCTTCTTGCCGTGCTCAGTGTATGGGCTGATATATTc                                                                                <  2:2324688/100‑1 (MQ=255)
                   aTCGGTGAGGATTTTTAACACCTTAAGAGGAATGGAAACCGTAATCTTTTTGACTTGTTCACTCTTCTTGCCGTGCTCAGTGTATGGGCTGATATATTcg                                                                               <  2:2423557/100‑1 (MQ=255)
                    tCGGTGAGGATTTTTAACACCTTAAGAGGAATGGAAACCGTAATCTTTTTGACTTGTTCACTCTTCTTGCCGTGCTCAGTGTATGGGCTGATATATTcgc                                                                              <  2:1537724/100‑1 (MQ=255)
                      ggTGAGGATTTTTAACACCTTAAGAGGAATGGAAACCGTAATCTTTTTGACTTGTTCACTCTTCTTGCCGTGCTCAGTGTATGGGCTGATATATTcgccg                                                                            >  1:973393/1‑100 (MQ=255)
                      ggTGAGGATTTTTAACACCTTAAGAGGAATGGAAACCGTAATCTTTTTGACTTGTTCACTCTTCTTGCCGTGCTCAGTGTATGGGCTGATATATTcgccg                                                                            >  2:1729395/1‑100 (MQ=255)
                      ggTGAGGATTTTTAACACCTTAAGAGGAATGGAAACCGTAATCTTTTTGACTTGTTCACTCTTCTTGCCGTGCTCAGTGTATGGGCTGATATATTcgccg                                                                            >  2:1050510/1‑100 (MQ=255)
                      ggTGAGGATTTTTAACACCTTAAGAGGAATGGAAACCGTAATCTTTTTGACTTGTTCACTCTTCTTGCCGTGCTCAGTGTATGGGCTGATATATTcgccg                                                                            >  2:1263421/1‑100 (MQ=255)
                      ggTGAGGATTTTTAACACCTTAAGAGGAATGGAAACCGTAATCTTTTTGACTTGTTCACTCCTCTTGCCGTGCTCAGCGTATGGGCTGATATATTcgccg                                                                            >  2:1790510/1‑100 (MQ=255)
                        tGAGGATTTTTAACACCTTAAGAGGAATGGAAACCGTAATCTTTTTGACTTGTTCACTCTTCTTTCCGTGCTCAGTGTATGGGCTGATATATTcg                                                                               <  1:1007774/95‑1 (MQ=255)
                        tGAGGATTTTTAACACCTTAAGAGGAATGGAAACCGTAATCTTTTTGACTTGTTCACTCTTCTTGCCGTGCTCAGTGTATGGGCTGATATATTCGCCGCt                                                                          <  1:555670/100‑1 (MQ=255)
                         gAGGATTTTTAACACCTTAAGAGGAATGGAAACCGTAATCTTTTTGACTTGTTCACTATTCTTGCCGTGCTCAGTGTATGGGCTGATATATTCGCCGCt                                                                          >  1:1833143/1‑99 (MQ=255)
                          aGGATTTTTAACACCTTAAGAGGAATGGAAACCGTAATCTTTTTGACTTGTTCACTCTTCTTGCCGTGCTCAGTGTATGGGCTGATATATTCGCCGCTcc                                                                        <  2:559364/100‑1 (MQ=255)
                           ggATTTTTAACACCTTAAGAGGAATGGAAACCGTAATCTTTTTGACTTGTTCACTCTTCTTGCCGTGCTCAGTGTATGGGCTGATATATTCGCCGCTCCa                                                                       >  2:1674792/1‑100 (MQ=255)
                           ggATTTTTAACACCTTAAGAGGAATGGAAACCGTAATCTTTTTGACTTGTTCACTCTTCTTGCCGTGCTCAGTGTATGGGCTGATATATTCGCCGCTCCa                                                                       >  2:1874090/1‑100 (MQ=255)
                           ggATTTTTAACACCTTAAGAGGAATGGAAACCGTAATCTTTTTGACTTGTTCACTCGTCTTCCCGTGCTCAGGGTATGGGCTGATACATGcgccgataca                                                                       >  2:2756335/1‑95 (MQ=255)
                            gATTTTTAACACCTTAAGAGGAATGGAAACCGTAATCTTTTTGACTTGTTCACTCTTCTTGCCGTGCTCAGTGTATGGGCTGATATATTCGCCGCTCCAt                                                                      >  1:2691346/1‑100 (MQ=255)
                            gATTTTTAACACCTTAAGAGGAATGGAAACCGTAATCTTTTTGACTTGTTCACTCTTCTTGCCGTGCCCAGCGTATGGGCTGATATATTCGCCGCTCCAt                                                                      >  1:1096986/1‑100 (MQ=255)
                             aTTTTTAACACCTTAAGAGGAATGGAAACCGTAATCTTTTTGACTTGTTCACTCTTCTTGCCGTGCTCAGTGTATGGGCTGATATATTCGCCGCTCCAtt                                                                     >  2:2879789/1‑100 (MQ=255)
                             aTTTTTAACACCTTAAGAGGAATGGAAACCGTAATCTTTTTGACTTGTTCACTCTTCTTGCCGTGCTCAGTGTATGGGCTGATATATTCGCCGCTCCAtt                                                                     <  2:1130646/100‑1 (MQ=255)
                              tttttAACACCTTAAGAGGAATGGAAACCGTAATCTTTTTGACTTGTTCACTCTTCTTGCCGTGCTCAGTGTATGGGCTGATATATTCGCCGCTCCATTc                                                                    >  2:1967541/1‑100 (MQ=255)
                              tttttAACACCTTAAGAGGAATGGAAACCGTAATCTTTTTGACTTGTTCACTCTTCTTGCCGTGCTCAGTGTATGGGCTGATATATTCGCCGCTCCATTc                                                                    <  2:1577801/100‑1 (MQ=255)
                                tttAACACCTTAAGAGGAATGGAAACCGTAATCTTTTTGACTTGTTCACTCTTCTTGCCGTGCTCAGTGTATGGGCTGATATATTCGCCGCTCCATTCAg                                                                  <  1:1976934/100‑1 (MQ=255)
                                tttAACACCTTAAGAGGAATGGAAACCGTAATCTTTTTGACTTGTTCACTCTTCTTGCCGTGCTCAGTGTATGGGCTGATATATTCGCCGCTCCATTCAg                                                                  <  1:1263415/100‑1 (MQ=255)
                                 ttAACACCTTAAGAGGAATGGAAACCGTAATCTTTTTGACTTGTTCACTCTTCTTGCCGTGCTCAGCGTATGGGCTGATATATTCGCCGCTCCATTCAGc                                                                 >  2:2398408/1‑100 (MQ=255)
                                  tAACACCTTAAGAGGAATGGAAACCGTAATCTTTTTGACTTGTTCACTCTTCTTGCCGTGCTCAGTGTATGGGCTGATATATTCGCCGCTCCATTCAGcc                                                                <  2:336847/100‑1 (MQ=255)
                                   aaCACCTTAAGAGGAATGGAAACCGTAATCTTTTTGACTTGTTCACTCTTCTTGCCGTGCTCAGTGTATGGGCTGATATATTCGCCGCTCCATTCAGccn                                                               <  1:2525408/100‑2 (MQ=255)
                                    acacCTTAAGAGGAATGGAAACCGTAATCTTTTTGACTTGTTCACTCTTCTTGCCGTGCTCAGTGTATGGGCTGATATATTCGCCGCTCCATTCAGCCAt                                                              >  1:389687/1‑100 (MQ=255)
                                    acacCTTAAGAGGAATGGAAACCGTAATCTTTTTGACTTGTTCACTCTTCTTGCCGTGCTCAGTGTATGGGCTGATATATTCGCCGCTCCATTCAGCCAt                                                              <  2:11623/100‑1 (MQ=255)
                                     cacCTTAATAGGAATGGATATCGTAATCTTTTTGACTTTTTCACTCTTCTTGCCGTGCTCAGTGTATGGGCTGATATATTCGCCGCTCCATTCAGCCa                                                               <  1:1403601/98‑1 (MQ=255)
                                     cacCTTAAGAGGAATGGAAACCGTAATCTTTTTGACTTGTTCACTCTTCTTGCCGTGCTCAGTGTATGGGCTGATATATTCGCCGCTCCATTCAGCCATg                                                             >  2:2496040/1‑100 (MQ=255)
                                     cacCTTAAGAGGAATGGAAACCGTAATCTTTTTGACTTGTTCACTCTTCTTGCCGTGCCCAGCGTATGGGCTGATATATTCGCCGCTCCATTCAGCCATg                                                             <  1:1487140/100‑1 (MQ=255)
                                     cacCTTAAGAGGAATGGAAACCGTAATCTCTTTGACTTGTTCACTCTTCTTGCCGTGCTCAGTGTACGGGCTGATATATTCGCCGCTCCATTCAGCCATg                                                             <  1:1837354/100‑1 (MQ=255)
                                      acCTTAAGAGGAATGGAAACCGTAATCTTTTTGACTTGTTCACTCTTCTTGCCGTGCTCAGTGTATGGGCTGATATATTCGCCGCTCCATTCAGCCATga                                                            <  2:680378/100‑1 (MQ=255)
                                       ccTTAAGAGGAATGGAAACCGTAATCTTTTTGACTTGTTCACTGTTCTTGCCGTGCTCAGTGTATGGGCTGATATATTCGCCGCTCCATTCAGCCATgag                                                           <  1:1956210/100‑1 (MQ=255)
                                       ccTTAAGAGGAATGGAAACCGTAATCTTTTTGACTTGTTCACTCTTCTTGCCGTGCTCAGTGTATGGGCTGATATATTCGCCGCTCCATTCAGCCATgag                                                           >  1:1087072/1‑100 (MQ=255)
                                       ccTTAAGAGGAATGGAAACCGTAATCTTTTTGACTTGTTCACTCTTCTTGCCGTGCTCAGTGTATGGGCTGATATATTCGCCGCTCCATTCAGCCATgag                                                           <  2:786459/100‑1 (MQ=255)
                                        cTTAAGAGGAATGGAAACCGTAATCTTTTTGACTTGTTCACTCTTCTTGCCGTGCTCAGTGTATGGGCTGATATATTCGCCGCTCCATTCAGCCATgaga                                                          >  1:395006/1‑100 (MQ=255)
                                         ttAAGAGGAATGGAAACCGTAATCTTTTTGACTTGTTCACTCTTCTTGCCGTGCTCAGTGTATGGGCTGATATATTCGCCGCTCCATTCAgatcggaaga                                                         >  1:2226947/1‑91 (MQ=255)
                                           aaGAGGAATGGAAACCGTAATCTTTTTGACTTGTTCACTCTTCTTGCCGTGCTCAGTGTATGGGCTGATATATTCGCCGCTCCATTCAGCCATGAGATAc                                                       <  2:2548623/100‑1 (MQ=255)
                                            agagGAATGGAAACCGTAATCTTTTTGACTTGTTCACTCTTCTTGCCGTGCTCAGTGTATGGGCTGATATATTCGCCGCTCCATTCAGCCATGAGATACt                                                      <  1:3030599/100‑1 (MQ=255)
                                            agagGAATGGAAACCGTAATCTTTTTGACTTGTTCACTCTTCTTGCCGTGCTCAGTGTATGGGCTGATATATTCGCCGCTCCATTCAGCCATGAGATACt                                                      <  2:321429/100‑1 (MQ=255)
                                             gagGAATGGAAACCGTAATCTTTTTGACTTGTTCACTCTTCTTGCCGTGCTCAGCGTATGGGCTGATATATTCGCCGCTCCATTCAGCCATGAGATACtt                                                     <  2:1659500/100‑1 (MQ=255)
                                               ggAATGGAAACCGTAATCTTTTTGACTTGTTCACTCTTCTTGCCGTGCTCAGTGTATGGGCTGATATATTCGCCGCTCCATTCAGCCATGAGATACTTaa                                                   <  1:2034202/100‑1 (MQ=255)
                                               ggAATGGAAACCGTAATCTTTTTGACTTGTTCACTCTTCTTGCCGTGCTCAGTGTATGGGCTGATATATTCGCCGCTCCATTCAGCCATGAGATACTTaa                                                   >  2:541771/1‑100 (MQ=255)
                                               ggAATGGAAACCGTAATCTTTTTGACTTGTTCACTCTTCTTGCCGTGCTCAGTGTATGGGCTGATATATTCGCCGCTCCATTCAGCCATGAGATACTTaa                                                   <  1:1300362/100‑1 (MQ=255)
                                                gAATGGAAACCGTAATCTTTTTGACTTGTTCACTCTTCTTGCCGTGCTCAGTGTATGGGCTGATATATTCGCCGCTCCATTCAGCCATGAGATACTTAAt                                                  <  2:1138868/100‑1 (MQ=255)
                                                 aaTGGAAACCGTAATCTTTTTGACTTGTTCACTCTTCTTGCCGTGCTCAGTGTATGGGCTGATATATTCGCCGCTCCATTCAGCCATGAGATACTTAATc                                                 >  1:1162164/1‑100 (MQ=255)
                                                  aTGGAAACCGTAATCTTTTTGACTTGTTCACTCTTCTTGCCGTGCTCAGTGTATGGGCTGATATATTCGCCGCTCCATTCAGCCATGAGATACTTAATcc                                                <  2:162168/100‑1 (MQ=255)
                                                    ggAAACCGTAATCTTTTTGACTTGTTCACTCTTCTTGCCGTGCTCAGTGTATGGGCTGATATATTCGCCGCTCCATTCAGCCATGAGATACTTAATCctc                                              >  2:1350240/1‑100 (MQ=255)
                                                         ccGTAATCTTTTTGACTTGTTCACTCTTCTTGCCGTGCTCAGTGTATGGGCTGATATATTCGCCGCTCCATTCAGCCATGAGATACTTAATCCTCTTCGt                                         <  1:8404/100‑1 (MQ=255)
                                                           gTAATCTTTTTGACTTGTTCACTCTTCTTGCCGTGCTCAGTGTATGGGCTGATATATTCGCCGCTCCATTCAGCCATGAGATACTTAATCCTCTTCGTCa                                       >  2:2244513/1‑100 (MQ=255)
                                                              aTCTTTTTGACTTGTTCACTCTTCTTGCCGTGCTCAGTGTATGGGCTGATATATTCGCCGCTCCATTCAGCCATGAGATACTTAATCCTCTTCGTCAATa                                    >  2:67523/1‑100 (MQ=255)
                                                                cTTTTTGACTTGTTCACTCTTCTTGCCGTGCTCAGTGTATGGGCTGATATATTCGCCGCTCCATTCAGCCATGAGATACTTAATCCTCTTCGTCAATaaa                                  >  1:591948/1‑100 (MQ=255)
                                                                 tttttGACTTGTTCACTCTTCTTGCCGTGCTCAGTGTATGGGCTGATATATTCGCCGCTCCATTCAGCCATGAGATACTTAATCCTCTTCGTCAATAAAt                                 >  2:432815/1‑100 (MQ=255)
                                                                 tttttGACTTGTTCACTCTTCTTGCCGTGCTCAGTGTATGGGCTGATATATTCGCCGCTCCATTCAGCCATGAGATACTTAATCCTCTTCGTCAATAAAt                                 <  1:669226/100‑1 (MQ=255)
                                                                  ttttGACTTGTTCACTCTTCTTGCCGTGCTCAGTGTATGGGCTGATATATTCGCCGCTCCATTCAGCCATGAGATACTTAATCCTCTTCGTCAATAAAtt                                <  2:19852/100‑1 (MQ=255)
                                                                  ttttGACTTGTTCACTCTTCTTGCCGTGCTCAGTGTATGGGCTGATATATTCGCCGCTCCATTCAGCCATGAGATACTTAATCCTCTTCGTCAATAAAtt                                <  2:2559148/100‑1 (MQ=255)
                                                                   tttGACTTGTTCACTCTTCTTGCCGTGCTCAGTGTATGGTCTGATATATTCGCCGCTCCATTCAGCCATGAGATACTTTATCCTCTTCGTCAATAAATTg                               <  1:2891281/100‑1 (MQ=255)
                                                                   tttGACTTGTTCACTCTTCTTGCCGTGCTCAGTGTATGGGCTGATATATTCGCCGCTCCATTCAGCCATGAGATACTTAATCCTCTTCGTCAATAAATTg                               <  2:1846886/100‑1 (MQ=255)
                                                                   tttGACTTGTTCACTCTTCTTGCCGTGCTCAGTGTATGGGCTGATATATTCGCCGCTCCATTCAGCCATGAGATACTTAATCCTCTTCGTCAATAAATTg                               <  2:2163849/100‑1 (MQ=255)
                                                                   tttGACTTGTTCACTCTTCTTGCCGTGCTCAGTGTATGGGCTGATATATTCGCCGCTCCATTCAGCCATGAGATACTTAATCCTCTTCGTCAATAAATTg                               <  1:1874079/100‑1 (MQ=255)
                                                                   tttGACTTGTTCACTCTTCTTGCCGTGCCCAGCGTATGGGCTGATATATTCGCCGCTCCATTCAGCCATGAGATACTTAATCCTCTTCGTCAATAAATTg                               <  2:1096992/100‑1 (MQ=255)
                                                                    ttGACTTGTTCACTCTTCTTGCCGTGCTCAGTGTATGGGCTGATATATTCGCCGCTCCATTCAGCCATGAGATACTTAATCCTCTTCGTCAATAAATTga                              >  1:1439363/1‑100 (MQ=255)
                                                                     tGACTTGTTCACTCTTCTTGCCGTGCTCAGTGTATGGGCTGATATATTCGCCGCTCCATTCAGCCATGAGATACTTAATCCTCTTCGTCAATAAATTgag                             >  2:2262892/1‑100 (MQ=255)
                                                                     tGACTTGTTCACTCTTCTTGCCGTGCTCAGCGTATGGGCTGATATATTCGCCGCTCCATTCAGCCATGAGATACTTAATCCTCTTCGTCAATAAATTgag                             <  1:2398395/100‑1 (MQ=255)
                                                                      gACTTGTTCACTCTTCTTGCCGTGCTCAGTGTATGGGCTGATATATTCGCCGCTCCATTCAGCCATGAGATACTTAATCCTCTTCGTCAATAAATTgaga                            <  2:2450805/100‑1 (MQ=255)
                                                                      gACTTGTTCACTCTTCTTCCCTTGCTCAGTGTATGGGCTGATATATTCGCCGCTCCATTCAGCCATGAGATACTTAATCCTCTTCGTCAATAAATTgag                             <  1:2689091/99‑1 (MQ=255)
                                                                       aCTTGTTCACTCTTCTTTTCTTGCTCAGTGTATGGTCTGATATATTCACCGCTCCATTCAGCCATGAGATACTTAATCCTCTTCGTCAATAAATTGagac                           <  2:890348/100‑1 (MQ=255)
                                                                        cTTGTTCACTCTTCTTGCCGTGCCCAGCGTATGGGCTGATATATTCGCCGCTCCATTCAGCCATGAGATACTTAATCCTCTTCGTCAATAAATTGagacc                          <  2:1095340/100‑1 (MQ=255)
                                                                             tCACTCTTCTTGCCGTGCTCAGTGTATGGGCTGATATATTCGCCGCTCCATTCAGCCATGAGATACTTAATCCTCTTCGTCAATAAATTGagaccagacc                     <  1:2667337/100‑1 (MQ=255)
                                                                              cACTCTTCTTGCCGTGCTCAGTGTATGGGCTGATATATTCGCCGCTCCATTCAGCCATGAGATACTTAATCCTCTTCGTCAATAAATTGAgaccagccca                    >  1:1330088/1‑100 (MQ=255)
                                                                              cACTCTTCTTGCCGTGCTCAGTGTATGGGCTGATATATTCGCCGCTCCATTCAGCCATGAGATACTTAATCCTCTTCGTCAATAAATTGAgaccagacca                    >  2:2999661/1‑100 (MQ=255)
                                                                                    tcttGCCGTGCTCAGTGTATGGGCTGATATATTCGCCGCTCCATTCAGCCATGAGATACTTAATCCTCTTCGTCAATAAATTGAGACCAGACCACACAGt              <  1:2244500/100‑1 (MQ=255)
                                                                                    tcttGCCGTGCTCAGTGTATGGGCTGATATATTCGCCGCTCCATTCAGCCATGAGATACTTAATCCTCTTCGTCAATAAATTGAGACCAGACCACACAGt              >  2:1597187/1‑100 (MQ=255)
                                                                                       tGCCGTGCTCAGTGTATGGGCTGATATATTCGCCGCTCCATTCAGCCATGAGATACTTAATCCTCTTCGTCAATAAATTGAGACCAGACCACACAGTTGa           >  1:2985522/1‑100 (MQ=255)
                                                                                        nccGTGCTCAGTGTATGGGCTGATATATTCGCCGCTCCATTCAGCCATGAGATACTTAATCCTCTTCGTCAATAAATTGAGACCAGACCACACAGTTGAt          >  1:2948583/2‑100 (MQ=255)
                                                                                         ccGTGCTCAGTGTGTGGGCTGATATATTCGCCGCTCCATTCAGCCATGAGATACTTAATCCTCTTCGTCAATAAATTGAGACCAGACCACACAGTTGAtg         >  2:1207352/1‑100 (MQ=255)
                                                                                         ccGTGCTCAGCGTATGGGCTGATATATTCGCCGCTCCATTCAGCCATGAGATACTTAATCCTCTTCGTCAATAAATTGAGACCAGACCACACAGTTGAtg         >  2:2741760/1‑100 (MQ=255)
                                                                                           gTGCTCAGTGTATGGGCTGATATATTCGCCTCTACATTCAGCCATGAGATACTTAATCCTCTTCGTCAATAAATTGAGACCAGACCACATAGTTGAtgtg       >  1:2003754/1‑100 (MQ=255)
                                                                                           gTGCTCAGTGTATGGGCTGATATATTCGCCGCTCCATTCAGCCATGAGATACTTAATCCTCTTCGTCAATAAATTGAGACCAGACCACACAGTTGAtgtg       <  2:395010/100‑1 (MQ=255)
                                                                                            tGCTCAGTGTATGGGCTGATATATTCGCCGCTCCATTCAGCCATGAGATACTTAATCCTCTTCGTCAATAAATTGAGACCAGACCACACAGTTGATGTgg      <  2:1651463/100‑1 (MQ=255)
                                                                                              cTCAGTGTATGGGCTGATATATTCGCCGCTCCATTCAGCCATGAGATACTTAATCCTCTTCGTCAATAAATTGAGACCAGACCACACAGTTGATGTGGGt    <  2:1724851/100‑1 (MQ=255)
                                                                                               tCAGTGTATGGGCTGATATATTCGCCGCTCCATTCAGCCATGAGATACTTAATCCTCTTCGTCAATAAATTGAGACCAGACCACACAGTTGATGTGGGTa   <  2:1439369/100‑1 (MQ=255)
                                                                                                cAGTGTATGGGCTGATATATTCGCCGCTCCATTCAGCCATGAGATACTTAATCCTCTTCGTCAATAAATTGAGACCAGACCACACAGTTGATGTGGGTAc  <  1:382965/100‑1 (MQ=255)
                                                                                                cAGTGTATGGGCTGATATATTCGCCGCTCCATTCAGCCATGAGATACTTAATCCTCTTCGTCAATAAATTGAGACCAGACCACACAGTTGATGTGGGTAc  >  2:1496933/1‑100 (MQ=255)
                                                                                                   |                                                                                                
CCTGACGACGCGTGCGTTCATCGGTGAGGATTTTTAACACCTTAAGAGGAATGGAAACCGTAATCTTTTTGACTTGTTCACTCTTCTTGCCGTGCTCAGCGTATGGGCTGATATATTCGCCGCTCCATTCAGCCATGAGATACTTAATCCTCTTCGTCAATAAATTGAGACCAGACCACACAGTTGATGTGGGTAC  >  NC_000913/4128260‑4128455

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A