Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A10 F210 I1 R1 75 123.0 6181441 93.9% 5804373 99.9

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 4,060,280 (T)9→8 intergenic (+50/‑167) typA → / → yihL GTP‑binding protein/putative DNA‑binding transcriptional regulator

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,060,2720T.95.1% 412.7 / 14.0 102intergenic (+42/‑175)typA/yihLGTP‑binding protein/putative DNA‑binding transcriptional regulator
Reads supporting (aligned to +/- strand):  ref base T (3/2);  new base . (57/40);  total (60/42)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.52e-01

Only 100 of 134 total aligned reads displayed.

ACGTCACCTGACGGAAAACGATCGTCGCCGCGCCAACCGCGCACCGAAAGACGATTAATTTCGTTTTTTAGTCATAAAAAACCTGCCAGCGATGGCAGG‑TTTTTTTTTGCCTGTCGTGAGCGCACACCAATGCGAGTATCTCCCTTATAAGTCTGTGGTTACGTCAATAGAGAGCTTTGTCACAATTATCTGCA  >  NC_000913/4060173‑4060366
                                                                                                    |                                                                                              
aCGTCACCTGACGGAAAACGATCGTCGCCGCGCCAACCGCGCACCGAAAGACGATTAATTTCGTTTTTTAGTCATAAAAAACCTGCCATCGCTGGCAGG‑t                                                                                                <  1:2351395/100‑1 (MQ=255)
 cGTCACCTGACGGAAAACGATCGTCGCCGCGCCAACCGCGCACCGAAAGACGATTAATTTCGTTTTTTAGTCATAAAAAACCTGCCATCGCTGGCAGG‑tt                                                                                               <  1:1006009/100‑1 (MQ=255)
 cGTCACCTGACGGAAAACGATCGTCGCCGCGCCAACCGCGCACCGAAAGACGATTAATTTCGTTTTTTAGTCATAAAAAACCTGCCAGCGATGGCAGG‑tt                                                                                               <  2:2986443/100‑1 (MQ=255)
   tCACCTGACGGAAAACGATCGTCGCCGCGCCAACCGCGCACCGAAAGACGATTAATTTCGTTTTTTAGTCATAAAAAACCTGCCATCGCTGGCAGG‑tttt                                                                                             <  1:1497828/100‑1 (MQ=255)
   tCACCTGACGGAAAACGATCGTCGCCGCGCCAACCGCGCACCGAAAGACGATTAATTTCGTTTTTTAGTCATAAAAAACCTGCCATCGCTGGCAGG‑tttt                                                                                             <  1:2898559/100‑1 (MQ=255)
   tCACCTGACGGAAAACGATCGTCGCCGCGCCAACCGCGCACCGAAAGACGATTAATTTCGTTTTTTAGTCATAAAAAACCTGCCATCGCTGGCAGG‑tttt                                                                                             <  1:1033429/100‑1 (MQ=255)
    caacTGACGGAAAACGATCGTCGCCGCGCCAACCGCGCACCGAAAGACGATTAATTTCGTTTTTTAGTCATAAAAATCCTGCCATCGCTGGCAGG‑tttt                                                                                             <  2:1791282/96‑1 (MQ=39)
    cACCTGACGGAAAACGATCGTCGCCGCGCCAACCGCGCACCGAAAGACGATTAATTTCGTTTTTTAGTCATAAAAAACCTGCCAGCGATGGCAGG‑ttttt                                                                                            >  2:1477670/1‑100 (MQ=255)
    cACCTGACGGAAAACGATCGTCGCCGCGCCAACCGCGCACCGAAAGACGATGAATTTCGTTTTTTAGTCATAAAAAACCTGCCAGCGATGGCAGG‑ttttt                                                                                            <  2:315987/100‑1 (MQ=255)
     aCCTGACGGAAAACGATCGTCGCCGCGTTATCCGCGCACCGAAAGACGATTAATTTCGTTTTTTAGTCATAAAAGACCTGCCATCGCTGGCAGG‑tttttt                                                                                           <  2:2449584/100‑1 (MQ=38)
     aCCTGACGGAAAACGATCGTCGCCGCGCCAACCGCGCACCGAAAGACGATTAATTTCGTTTTTTAGTCATAAAAAACCTGCCATCGCTGGCAGG‑tttttt                                                                                           <  2:2552036/100‑1 (MQ=255)
       cTGACGGAAAACGATCGTCGCCGCGCCAACCGCGCACCGAAAGACGATTAATTTCGTTTTTTAGTCATAAAAAACCTGCCAGCGATGGCAGG‑tttttttt                                                                                         <  1:620805/100‑1 (MQ=255)
        tGACGGAAAACGATCGTCGCCGCGCCAACCGCGCACCGAAAGACGATTAATTTCGTTTTTTAGTCATAAAAAACCTGCCATCGCTGGCAGG‑ttttttatg                                                                                        <  2:1468501/100‑4 (MQ=38)
        tGACGGAAAACGATCGTCGCCGCGCCAACCGCGCACCGAAAGACGATTAATTTCGTTTTTTAGTCATAAAAAACCTGCCAGCGATGGCAGG‑ttttttttg                                                                                        >  1:3010622/1‑99 (MQ=255)
        tGACGGAAAACGATCGTCGCCGCGCCAACCGCGCACCGAAAGACGATTAATTTCGTTTTTTAGTCATAAAAAACCTGCCAGCGATGGCAGG‑ttttttttg                                                                                        <  1:2459968/100‑2 (MQ=255)
         gACGGAAAAGGATCGTCGCCGCGCCTACCGCGGACCGAAAGCCGATTAATTTCGTTTTTTAGTCATAGAAAACCTGCCAGCGATGGCAGG‑ttttttttg                                                                                        <  1:996625/99‑2 (MQ=255)
          aCGGAAAACGATCGTCGCCGCGCCAACCGCGCACCGAAAGACGATTAATTTCGTTTTTTAGTCATAAAAACCCTGCCAGCGATGGCAGG‑TTTTTTTTTGc                                                                                      <  1:240515/100‑1 (MQ=255)
          aCGGAAAACGATCGTCGCCGCGCCAACCGCGCACCGAAAGACGATTAATTTCGTTTTTTAGTCATAAAAAACCTGCCAGCGATGGCAGG‑ttttttttgcc                                                                                      <  2:2878831/100‑4 (MQ=255)
          aCGGAAAACGATCGTCGCCGCGCCAACCGCGCACCGAAAGACGATTAATTTCGTTTTTTAGTCATAAAAAACCTGCCAGCGATGGCAGG‑ttttttttgcc                                                                                      <  1:2225741/100‑4 (MQ=255)
           cGGAAAACGATCGTCGCCGCGCCAACCGCGCACCGAAAGACGATTAATTTCGTTTTTTAGTCATAAAAAACCTGCCAGCGATGGCAGG‑ttttttttgcct                                                                                     >  2:2580466/1‑96 (MQ=255)
           cGGAAAACGATCGTCGCCGCGCCAACCGCGCACCGAAAGACGATTAATTTCGTTTTTTAGTCATAAAAAACCTGCCAGCGATGGCAGG‑ttttttttgcct                                                                                     >  1:875251/1‑96 (MQ=255)
            ggAAAACGATCGTCGCCGCGCCAACCGCGCACCGAAAGACGATTAATTTCGTTTTTTAGTCATAAAAAACCTGCCAGCGATGGCAGG‑‑TTTTTTTTGCCTg                                                                                   >  2:1748902/1‑100 (MQ=255)
            ggAAAACGATCGTCGCCGCGCCAACCGCGCACCGAAAGACGATTAATTTCGTTTTTTAGTCATAAAAAACCTGCCAGCGATGGCAGG‑‑TTTTTTTTGCCTg                                                                                   >  1:528895/1‑100 (MQ=255)
             gAAAACGATCGTCGCCGCGCCAACCGCGCACCGAAAGACGATTAATTTCGTTTTTTAGTCATAAAAAACCTGCCAGCGATGGAAGG‑ttttttttgcct                                                                                     >  1:2884176/1‑94 (MQ=255)
                 aCGATCGTCGCCGCGCCAACCGCGCACCGAAAGACGATTAATTTCGTTTTTTAGTCATAAAAAACCTGCCAGCGATGGCAGG‑‑TTTTTTTTGCCTGTCGTg                                                                              <  2:191167/100‑1 (MQ=255)
                 aCGATCGTCGCCGCGCCAACCGCGCACCGAAAGACGATTAATTTCGTTTTTTAGTCATAAAAAACCTGCCAGCGATGGCAGG‑‑TTTTTTTTGCCTGTCGTg                                                                              <  2:480308/100‑1 (MQ=255)
                   gATCGTCGCCGCGCCAACCGCGCACCGAAAGACGATTAATTTCGTTTTTTAGTCATAAAAAACCTGCCAGCGATGGCAGG‑‑TTTTTTTTGCCTGTCGTGAg                                                                            >  1:392194/1‑100 (MQ=255)
                    aTCGTCGCCGCGCCAACCGCGCACCGAAAGACGATTAATTTCGTTTTTTAGTCATAAAAAACCTGCCAGCGATGGCAGG‑‑TTTTTTTTGCCTGTCGTGAgc                                                                           >  1:1891760/1‑100 (MQ=255)
                    aTCGTCGCCGCGCCAACCGCGCACCGAAAGACGATTAATTTCGTTTTTTAGGCATAAAAAACCTGCCAGCGATGGCAGG‑‑TTTTTTTTGCCTGTCGTGAgc                                                                           <  1:131704/100‑1 (MQ=255)
                       gtcgCCGCGCCAACCGCGCACCGAAAGACGATTAATTTCGTTTTTTAGTCATAAAAAACCTGCCAGCGATGGCAGG‑‑TTTTTTTTGCCTGTCGTGAGCGca                                                                        <  2:1707877/100‑1 (MQ=255)
                       gtcgCCGCGCCAACCGCGCACCGAAAGACGATTAATTTCGTTTTTTAGTCATAAAAAACCTGCCAGCGATGGCAGG‑‑TTTTTTTTGCCTGTCGTGAGCGca                                                                        >  1:45053/1‑100 (MQ=255)
                          gccgcGCCAACCGCGCACCGAAAGACGATTCATTTCGTTTTTTAGTCATAAAAAACCTGCCAGCGATGGCAGG‑‑TTTTTTTTGCCTGTAGTGAGCGCacac                                                                     >  2:1335135/1‑100 (MQ=255)
                          gccgcGCCAACCGCGCACCGAAAGACGATTAATTTCGTTTTTTAGTCATAAAAAACCTGCCAGCGATGGCAGG‑‑TTTTTTTTGCCTGTCGTGAGCGCacac                                                                     >  2:1330616/1‑100 (MQ=255)
                          gccgcGCCAACCGCGCACCGAAAGACGATTAATTTCGTTTTTTAGTCATAAAAAACCTGCCAGCGATGGCAGG‑‑TTTTTTTTGCCTGTCGTGAGCGCacac                                                                     <  1:960796/100‑1 (MQ=255)
                           ccgcGCCAACCGCGCACCGAAAGACGATTAATTTCGTTTTTTAGTCATAAAAAACCTGCCAGCGATGGCAGG‑‑TTTTTTTTGCCTGTCGTGAGCGCACAcc                                                                    <  2:1469031/100‑1 (MQ=255)
                           ccgcGCCAACCGCGCACCGAAAGACGATTAATTTCGTTTTTTAGTCATAAAAAACCTGCCAGCGATGGCAGG‑‑TTTTTTTTGCCTGTCGTGAGCGCACAcc                                                                    >  1:2095429/1‑100 (MQ=255)
                             gcgcCAACCGCGCACCGAAAGACGATTAATTTCGTTTTTTAGTCATAAAAAACCTGCCAGCGATGGCAGG‑‑TTTTTTTTGCCTGTCGTGAGCGCACACCaa                                                                  >  1:1972700/1‑100 (MQ=255)
                             gcgcCAACCGCGCACCGAAAGACGATTAATTTCGTTTTTTAGTCATAAAAAACCTGCCAGCGATGGCAGG‑‑TTTTTTTTGCCTGTCGTGAGCGCACACCaa                                                                  >  1:735902/1‑100 (MQ=255)
                                     cgcgCACCGAAAGACGATTAATTTCGTTTTTTAGTCATAAAAAACCTGCCAGCGATGGCAGG‑‑TTTTTTTTGCCTGTCGTGAGCGCACACCAATGCGAGTa                                                          <  1:2746547/100‑1 (MQ=255)
                                      gcgcACCGAAAGACGATTAATTTCGTTTTTTAGTCATAAAAAACCTGCCAGCGATGGCAGG‑‑TTTTTTTTGCCTGTCGTGAGCGCACACCAATGCGAGTAt                                                         >  1:1139297/1‑100 (MQ=255)
                                        gcACCGAAAGACGATTAATTTCGTTTTTTAGTCATAAAAAACCTGCCAGCGATGGCAGG‑‑TTTTTTTTGCCTGTCGTGAGCGCACACCAATGCGAGTAtct                                                       >  2:1175631/1‑100 (MQ=255)
                                         cACCGAAAGACGATTAATTTCGTTTTTTAGTCATAAAAAACCTGCCAGCGATGGCAGG‑‑TTTTTTTTGCCTGTCGTGAGCGCACACCAATGCGAGTAtctc                                                      >  1:116135/1‑100 (MQ=255)
                                          aCCGAAAGACGATTAATTTCGTTTTTTAGTCATAAAAAACCTGCCAGCGATGGCAGG‑‑TTTTTTTTGCCTGTCGTGAGCGCACACCAATGCGAGTATCTcc                                                     <  1:595216/100‑1 (MQ=255)
                                          aCCGAAAGACGATTAATTTCGTTTTTTAGTCATAAAAAACCTGCCAGCGATGGCAGG‑‑TTTTTTTTGCCTGTCGTGAGCGCACACCAATGCGAGTATCTcc                                                     >  2:640668/1‑100 (MQ=255)
                                             gAAAGACGATTAATTTCGTTTTTTAGTCATAAAAAACCTGCCAGCGATGGCAGG‑‑TTTTTTTTGCCTGTCGTGAGCGCACACCAATGCGAGTATCTCCCtt                                                  >  1:1756867/1‑100 (MQ=255)
                                              aaaGACGATTAATTTCGTTTTTTAGTTATAAAATACCTGCCAGCGATGGCAGG‑‑TTTTTTTTGCCTGTCGTGAGCGCACACCAATGCGAGTATCTCCCTta                                                 >  2:2036013/1‑100 (MQ=255)
                                                aGACGATTAATTTCGTTTTTTAGTCATAAAAAACCTGCCAGCGATGGCAGG‑‑TTTTTTTTGCCTGTCGTGAGCGCACACCAATGCGAGTATCTCCCTtata                                               <  2:457631/100‑1 (MQ=255)
                                                 gACGATTAATTTCGTTTTTTAGTCATAAAAAACCTGCCAGCGATGGCAGG‑‑TTTTTTTTGCCTGTCGTGAGCGCACACCAATGCGAGTATCTCCCTTATaa                                              >  2:638461/1‑100 (MQ=255)
                                                  aCGATTAATTTCGTTTTTTAGTCATAAAAAACCTGCCAGCGATGGCAGG‑‑TTTTTTTTGCCTGTCGTGAGCGCACACCAATGCGAGTATCTCCCTTATAAg                                             <  1:1785428/100‑1 (MQ=255)
                                                      ttAATTTCGTTTTTTAGTCATAAAAAACCTGCCAGCGATGGCAGG‑‑TTTTTTTTGCCTGTCGTGAGCGCACACCAATGCGAGTATCTCCCTTATAAGTCtg                                         <  2:68463/100‑1 (MQ=255)
                                                        aaTTTCGTTTTTTAGTCATAAAAAACCTGCCAGCGATGGCAGG‑‑TTTTTTTTGCCTGTCGTGAGCGCACACCAATGCGAGTATCTCCCTTATAAGTCtgtg                                       <  1:1518052/100‑1 (MQ=255)
                                                         aTTTCGTTTTTTAGTCATAAAAAACCTGCCAGCGATGGCAGG‑‑TTTTTTTTGCCTGTCGTGAGCGCACACCAATGAGAGTATCTCCCTTATAAGTCTGTgg                                      >  2:1357360/1‑100 (MQ=255)
                                                          tttCGTTTTTTAGTCATAAAAAACCTGCCAGCGATGGCAGGTTTTTTTTTTGCCTGTCGTGAGCGCACACCAATGCGAGTATCTCCCTTATAAGTCtgtg                                       <  1:248533/100‑1 (MQ=255)
                                                          tttCGTTTTTTAATCATAAAAAACCTCCCAGCGATGGCAGG‑‑TTTTTTTTGCCTGTCGTGAGCGCACACCAATGCGAGTATCTCCCTTATAAGTCtgtg                                       <  2:1257039/98‑1 (MQ=255)
                                                           ttCGTTTTTTAGTCATAAAAAACCTGCCAGCGATGGCAGG‑‑TTTTTTTTGCCTGTCGTGAGCGCACACCAATGCGAGTATCTCCCTTATAAGTCTGTGGtt                                    >  2:2282702/1‑100 (MQ=255)
                                                            tCGTTTTTTAGTCATAAAAAACCTGCCAGCGATGGCAGG‑‑TTTTTTTTGCCTGTCGTGAGCGCACACCAATGCGAGTATCTCCCTTATAAGTCTGTGGTTa                                   >  1:2713619/1‑100 (MQ=255)
                                                              gTTTTTTAGTCATAAAAAACCTGCCAGCGATGGCAGG‑‑TTTTTTTTGCCTGTCGTGAGCGCACACCAATGCGAGTATCTCCCTTATAAGTCTGTGGTTACg                                 <  1:2580489/100‑1 (MQ=255)
                                                               ttttttAGTCATAAAAAACCTGCCAGCGATGGCAGG‑‑TTTTTTTTGCCTGTCGTGAGCGCACACCAATGCGAGTATCTCCCTTATAAGTCTGTGGTTACg                                 <  2:2095405/99‑1 (MQ=255)
                                                                tttttAGTCATAAAAAACCTGCCAGCGATGGCAGG‑‑TTTTTTTTGCCTGTCGTGAGCGCACACCAATGCGAGTATCTCCCTTATAAGTCTGTGGTTACGTc                               <  1:2993495/100‑1 (MQ=255)
                                                                tttttAGTCATAAAAAACCTGCCAGCGATGGCAGG‑‑TTTTTTTTGCCTGTCGTGAGCGCACACCAATGCGAGTATCTCCCTTATAAGTCTGTGGTTACGTc                               <  2:1939352/100‑1 (MQ=255)
                                                                 ttttAGTCATAAAAAACCTGCCAGCGATGGCAGG‑‑TTTTTTTTGCCTGTCGTGAGCGCACACCAATGCGAGTATCTCCCTTATAAGTCTGTGGTTACGTCa                              <  1:2725689/100‑1 (MQ=255)
                                                                   ttAGTCATAAAAAACCTGCCAGCGATGGCAGG‑‑TTTTTTTTGCCTGTCGTGAGCGCACACCAATGCGAGTATCTCCCTTATAAGTCTGTGGTTACGTCAAt                            >  2:2362148/1‑100 (MQ=255)
                                                                   acaggcaaaaaaaaCCTGCCATTGCTGGCAGG‑‑TTTTTTATGACTGTCGTGAGCGCACACCAATGCGAGTATCTCCCTTATAAGTCTGTGGTTACGTCAAt                            >  2:2942979/9‑100 (MQ=21)
                                                                     aGTCATAAAAAACCTGCCAGCGATGGCAGG‑‑TTTTTTTTGCCTGTCGTGAGCGCACACCAATGCGAGTATCTCCCTTATAAGTCTGTGGTTACGTCAATag                          <  2:735898/100‑1 (MQ=255)
                                                                      ggcaaaaaaaaCCTGCCATCGCTGGCAGG‑‑TTTTTTTTGCCTGTCGTGAGCGCACACCAATGCGAGTATCTCCCTTATAAGTCTGTGGTTACGTCAATaga                         >  2:1281704/6‑100 (MQ=37)
                                                                       tCATAAAAAACCTGCCAGCGATCGCAGG‑‑TTTCTTTTGTCTGTCGTGAACGCACACCAATGCGAGTATCTCCCTTATAAGTCTGTGGTTACGTCAATagag                        <  2:2084116/100‑1 (MQ=255)
                                                                       gcaaaaaaaaCCTGCCATCGCTGGCAGG‑‑TTTTTTTTGCCTGTCGTGAGCGCACACCAATGCGAGTATCTCCCTTATAAGTCTGTGGTTACGTCAATagag                        >  1:2908060/5‑100 (MQ=37)
                                                                        naTAAAAAACCTGCCAGCGATGGCAGG‑‑TTTTTTTTGCCTGTCGTGAGCGCACACCAATGCGAGTATCTCCCTTATAAGTCTGTGGTTACGTCAATagaga                       >  2:1640785/2‑100 (MQ=255)
                                                                        cATAAAAAACCTGCCAGCGATGGCAGG‑‑TTTTTTTTGCCTGTCGTGAGCGCACACCAATGCGAGTATCTCCCTTATAAGTCTGTGGTTACGTCAATagaga                       >  1:1370948/1‑100 (MQ=255)
                                                                        cATAAAAAACCTGCCAGCGATGGCAGG‑‑TTTTTTTTGCCTGTCGTGAGCGCACACCAATGCGAGTATCTCCCTTATAAGTCTGTGGTTACGTCAATagaga                       <  1:1073803/100‑1 (MQ=255)
                                                                         aaaaaaaaCCTGCCATCGCTGGCAGG‑‑TTTTTTTTGCCTGTCGTGAGCGCACACCAATGCGAGTATCTCCCTTATAAGTCTGTGGTTACGTCAATagagag                      >  2:2913138/3‑100 (MQ=38)
                                                                         aTAAAAAACCTGCCAGCGATGGCAGG‑‑TTTTTTTTGCCTGTCGTGAGCGCACACCAATGCGAGTATCTCCCTTATAAGTCTGTGGTTACGTCAATagagag                      >  1:2223231/1‑100 (MQ=255)
                                                                          tAAAAAACCTGCCAGCGATGGCAGG‑‑TTTTTTTTGCCTGTCGTGAGCGCACACCAATGCGAGTATCTCCCTTATAAGTCTGTGGTTACGTCAATAGAGAGc                     >  1:3055035/1‑100 (MQ=255)
                                                                          tAAAAAACCTGCCAGCGATGGCAGG‑‑TTTTTTTTGCCTGTCGTGAGCGCACACCAATGCGAGTATCTCACTTATAAGTATGTGGTTACGTCCATAGAGAGc                     >  2:1671397/1‑100 (MQ=255)
                                                                          aaaaaaaCCTGCCATCGCTGGCAGG‑‑TTTTTTTTGCCTGTCGTGAGCGCACACCAATGCGAGTATCTCCCTTATAAGTCTGTGGTTACGTCAATAGAGAGc                     <  2:2667292/99‑1 (MQ=38)
                                                                          aaaaaaaCCTGCCATCGCTGGCAGG‑‑TTTTTTTTGCCTGTCGTGAGCGCACACCAATGCGAGTATCTCCCTTATAAGTCTGTGGTTACGTCAATAGAGAGc                     >  1:2006426/2‑100 (MQ=38)
                                                                          aaaaaaaCCTGCCATCGCTGGCAGG‑‑TTTTTTTTGCCTGTCGTGAGCGCACACCAATGCGAGTATCTCCCTTATAAGTCTGTGGTTACGTCAATAGAGAGc                     >  2:2138226/2‑100 (MQ=38)
                                                                            aaaaaCCTGCCATCGCTGGCAGG‑‑TTTTTTTTGCCTGTCGTGAGCGCACACCAATGCGAGTATCTCCCTTATAAGTCTGTGGTTACGTCAATAGAGAGCtt                   >  1:1482859/1‑100 (MQ=38)
                                                                            aaaaaCCTGCCATCGCTGGCAGG‑‑TTTTTTTTGCCTGTCGTGAGCGCACACCAATGCGAGTATCTCCCTTATAAGTCTGTGGTTACGTCAATAGAGAGCtt                   >  1:914921/1‑100 (MQ=38)
                                                                            aaaaaCCTGCCATCGCTGGCAGG‑‑TTTTTTTTGCCTGTCGTGAGCGCACACCAATGCGAGTATCTCCCTTATAAGTCTGTGGTTACGTCAATAGAGAGCtt                   >  1:1436319/1‑100 (MQ=38)
                                                                            aaaaaCCTGCCAGCGATGGCAGG‑‑TTTTTTTTGCCTGTCGTGAGCGCACACCAATGCGAGTATCTCCCTTATAAGTCTGTGGTTACGTCAATAGAGAGCtt                   <  2:1285815/100‑1 (MQ=255)
                                                                            aaaaaCCTGCCAGCGATGGCAGG‑‑TTTTTTTTGCCTGTCGTGAGCGCACACCAATGCGAGTATCTCCCTTATAAGTCTGTGGTTACGTCAATAGAGAGCtt                   <  2:2764338/100‑1 (MQ=255)
                                                                             aaaaCCTGCCAGCGATGGCAGG‑‑TTTTTTTTGTTTTTTTTTAACGCACACCAATGCGAGTATCTTTCTTATTAGTCTGTGGTTCCGTCATTTGAGAGCttt                  >  2:1199639/1‑100 (MQ=255)
                                                                                aCCTGCCATCGCTGGCAGG‑‑TTTTTTTTGCCTGTCGTGAGCGCACACCAATGCGAGTATCTCCCTTATAAGTCTGTGGTTACGTCAATAGAGAGCTTTGTc               >  1:2641424/1‑100 (MQ=255)
                                                                                 ccTGCCATCGCTGGCAGG‑‑TTTTTTTTGCCTGTCGTGAGCGCACACCAATGCGAGTATCTCCCTTATAAGTCTGTGGTTACGTCAATAGAGAGCTTTGTc               >  1:1216455/1‑99 (MQ=255)
                                                                                 ccTGCCAGCGATGGCAGG‑‑TTTTTTTTGCCTGTCGTGAGCGCACACCAATGCGAGTATCTCCCTTATAAGTCTGTGGTTACGTCAATAGAGAGCTTTGTca              <  2:1587323/100‑1 (MQ=255)
                                                                                 catgacAGCGCTGGCAGG‑‑TTTTTTTTGCCTGTCGTGAGCGAACACCAATGAGAGTATCTCCAGTATAAGTCTGTGTTTACGTCAATAGAGACCTTGGTca              <  2:2884147/95‑1 (MQ=255)
                                                                                  cTGCCAGCGATGGCAGG‑‑TTTTTTTTGCCTGTCGTGAGCGCACACCAATGCGAGTATCTCCCTTATAAGTCTGTGGTTACGTCAATAGAGAGCTTTGTcac             <  2:45053/100‑1 (MQ=255)
                                                                                  cTGCCAGCGATGGCAGG‑‑TTTTTTTTGCCTGTCGTGAGCGCACACCAATGCGAGTATCTCCCTTATAAGTCTGTGGTTACGTCAATAGAGAGCTTTGTcac             <  1:2282725/100‑1 (MQ=255)
                                                                                    gCCAGCGATGGCAGG‑‑TTTTTTTTGCCTGTCGTGAGCGCACACCAATGCGAGTATCTCCCTTATAAGTCTGTGGTTACGTCAATAGAGAGCTTTGTCACaa           <  2:2425322/100‑1 (MQ=255)
                                                                                    gCCAGCGATGGCAGG‑‑TTTTTTTTGCCTGTCGTGAGCGCACACCAATGCGAGTATCTCCCTTATAAGTCTGTGGTTACGTCAATAGAGAGCTTTGTCACaa           >  1:2551138/1‑100 (MQ=255)
                                                                                     ccAGCGATGGCAGG‑‑TTTTTTTTGCCTGTCGTGAGCGCACACCAATGCGAGTATCTCCCTTATAAGTCTGTGGTTACGTCAATAGAGAGCTTTGTCACAAt          >  1:2990684/1‑100 (MQ=255)
                                                                                     ccAGCGATGGCAGG‑‑TTTTTTTTGCCTGTCGTGAGCGCACACCAATGCGAGTATCTCCCTTATAAGTCTGTGGTTACGTCAATAGAGAGCTTTGTCACAAt          <  1:607359/100‑1 (MQ=255)
                                                                                     ccAGCGATGGCAGG‑‑TTTTTTTTGCCTGTCGTGAGCGCACACCAATGCGAGTATCTCCCTTATAAGTCTGTGGTTACGTCAATAGAGAGCTTTGTCACAAt          >  1:2374581/1‑100 (MQ=255)
                                                                                       aGCGATGGCAGG‑‑TTTTTTTTGCCTGTCGTGAGCGCACACCAATGCGAGTATCTCCCTTATAAGTCTGTGGTTACGTCAATAGAGAGCTTTGTCACAATTa        >  1:731419/1‑100 (MQ=255)
                                                                                           aTGGCAGG‑‑TTTTTTTTGCCTGTCGTGAGCGCACACCAATGCGAGTATCTCCCTTATAAGTCTGTGGTTACGTCAATAGAGAGCTTTGTCACAATTATCTg    <  1:2415507/100‑1 (MQ=255)
                                                                                             ggCAGG‑‑TTTTTTTTGCCTGTCGTGAGCGCACACCAATGCGAGTATCTCCCTTATAAGTCTGTGGTTACGTCAATAGAGAGCTTTGTCACAATTATCTGCa  >  2:2784566/1‑100 (MQ=255)
                                                                                             ggCAGG‑‑TTTTTTTTGCCTGTCGTGAGCGCACACCAATGCGAGTATCTCCCTTATAAGTCTGTGGTTACGTCAATAGAGAGCTTTGTCACAATTATCTGCa  >  1:696926/1‑100 (MQ=255)
                                                                                             ggCAGG‑‑TTTTTTTTGCCTGTCGTGAGCGCACACCAATGCGAGTATCTCCCTTATAAGTCTGTGGTTACGTCAATAGAGAGCTTTGTCACAATTATCTGCa  >  1:698869/1‑100 (MQ=255)
                                                                                              gCAGG‑TTTTTTTTT‑TTTTTTTTGAGCGCACACCAATGCGAGTATCTTCCTTATAAGTCTGTGGTTACGTCAATAGAGAGCTTTGTCACAATTATCt     >  2:437057/1‑96 (MQ=255)
                                                                                                    |                                                                                              
ACGTCACCTGACGGAAAACGATCGTCGCCGCGCCAACCGCGCACCGAAAGACGATTAATTTCGTTTTTTAGTCATAAAAAACCTGCCAGCGATGGCAGG‑TTTTTTTTTGCCTGTCGTGAGCGCACACCAATGCGAGTATCTCCCTTATAAGTCTGTGGTTACGTCAATAGAGAGCTTTGTCACAATTATCTGCA  >  NC_000913/4060173‑4060366

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A