Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A10 F169 I1 R1 59 169.8 8365175 96.8% 8097489 99.9

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 4,060,280 (T)9→8 intergenic (+50/‑167) typA → / → yihL GTP‑binding protein/putative DNA‑binding transcriptional regulator

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,060,2720T.97.4% 506.1 / 5.9 117intergenic (+42/‑175)typA/yihLGTP‑binding protein/putative DNA‑binding transcriptional regulator
Reads supporting (aligned to +/- strand):  ref base T (1/2);  new base . (59/55);  total (60/57)
Fisher's exact test for biased strand distribution p-value = 6.12e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.72e-01

Only 100 of 154 total aligned reads displayed.

ACGTCACCTGACGGAAAACGATCGTCGCCGCGCCAACCGCGCACCGAAAGACGATTAATTTCGTTTTTTAGTCATAAAAAACCTGCCAGCGATGGCAGGTTTTTTTTTGCCTGTCGTGAGCGCACACCAATGCGAGTATCTCCCTTATAAGTCTGTGGTTACGTCAATAGAGAGCTTTGTCACAATTATCTGCAA  >  NC_000913/4060173‑4060367
                                                                                                   |                                                                                               
aCGTCACCTGACGGAAAACGATCGTCGCCGCGCCAACCGCGCACCGAAAGACGATTAATTTCGTTTTTTAGTCATAAAAAACCTGCCATCGCTGGCAGGt                                                                                                 <  2:2268583/100‑1 (MQ=255)
aCGTCACCTGACGGAAAACGATCGTCGCCGCGCCAACCGCGCACCGAAAGACGATTAATTTCGTTTTTTAGTCATAAAAAACCTGCCAGCGATGGCAGGt                                                                                                 <  1:3492530/100‑1 (MQ=255)
 cGTCACCTGACGGAAAACGATCGTCGCCGCGCCAACCGCGCACCGAAAGACGATTAATTTCGTTTTTTAGTCATAAAAAACCTGCCAGCGATGGCAGGtt                                                                                                <  2:834992/100‑1 (MQ=255)
  gTCACCTGACGGAAAACGATCGTCGCCGCGCCAACCGCGCACCGAAAGACGATTAATTTCGTTTTTTAGTCATAAAAAACCTGCCATCGCTGGCAGGttt                                                                                               <  1:2842475/100‑1 (MQ=255)
   tctccTGACGGAAAACGATCGTCGCCGCGTCAACCGCGTACCGAAAGACGTTTAATTTCGTTTTTTAGTCATAAAAAACCTGCCATCGCTGGCAGGtttt                                                                                              <  2:3404350/97‑1 (MQ=38)
   tCACCTGACGGAAAACGATCGTCGCCGCGCCAACCGCGCACCGAAAGACGATTAATTTCGTTTTTTAGTCATAAAAAACCTGCCAGCGATGGCAGGtttt                                                                                              >  1:102006/1‑100 (MQ=255)
    cACCTGACGGAAAACGATCGTCGCCGCGCCAACCGCGCACCGAAAGACGATTAATTTCGTTTTTTAGTCATAAAAAACCTGCCAGCGATGGCAGGttttt                                                                                             >  2:3338820/1‑100 (MQ=255)
    cACCTGACGGAAAACGATCGTCGCCGCGCCAACCGCGCACCGAAAGACGATTAATTTCGTTTTTTAGTCATAAAAAACCTGCCAGCGATGGCAGGttttt                                                                                             <  2:3277255/100‑1 (MQ=255)
     aCCTGACGGAAAACGATCGTCGCCGCGCCAACCGCGCACCGAAAGACGATTAATTTCGTTTTTTAGTCATAAAAAACCTGCCAGCGATGGCAGGtttttt                                                                                            <  1:226901/100‑1 (MQ=255)
      ttccgatctaaaCGATCGTCGCCGCGCCAACCGCGCACCGAAAGACGATTAATTTCGTTTTTTAGTCATAAAAAACCTGCCAGCGATGACAGGttttttt                                                                                           <  1:2921207/91‑1 (MQ=255)
      ccTGACGGAAAACGATCGTCGCCGCGCCAACCGCGCACCGAAAGACGATTAATTTCGTTTTTTAGTCATAAAAAACCTGCCAGCGATGGCAGGttttttt                                                                                           <  1:809960/100‑1 (MQ=255)
       cTGACGGAAAACGATCGTCGCCGCGCCAACCGCGCACCGAAAGACGATTAATTTCGTTTTTTAGTCATAAAAAACCTGCCATCGCTGGCAGGttttttat                                                                                          <  1:3829432/100‑3 (MQ=255)
       cTGACGGAAAACGATCGTCGCCGCGCCAACCGCGCACCGAAAGACGATTAATTTCGTTTTTTAGTCATAAAAAACCTGCCAGCGATGGCAGGtttttttt                                                                                          <  2:1163743/100‑1 (MQ=255)
       cTGACGGAAAACGATCGTCGCCGCGCCAACCGCGCACCGAAAGACGATTAATTTCGTTTTTTAGTCAAAAAAAACCTGCCATCGCTGGCAGGttttttat                                                                                          <  1:2025191/100‑3 (MQ=38)
        tGACGGAAAACGATCGTCGCCGCGCCAACCGCGCACCGAAAGACGATTAATTTCGTTTTTTAGTCATAAAAAACCTGCCAGCGATGGCAGGttttttttg                                                                                         >  1:10146/1‑99 (MQ=255)
        tGACGGAAAACGATCGTCGCCGCGCCAACCGCGCACCGAAAGACGATTAATTTCGTTTTTTAGTCATAAAAAACCTGCCAGCGATGGCAGGttttttttg                                                                                         >  1:2918922/1‑99 (MQ=255)
        tGACGGAAAACGATCGTCGCCGCGCCAACCGCGCACCGAAAGACGATTAATTTCGTTTTTTAGTCATAAAAAACCTGCCAGCGATGGCAGGttttttttg                                                                                         <  1:171733/100‑2 (MQ=255)
        tGACGGAAAACGATCGTCGCCGCGCCAACCGCGCACCGAAAGACGATTAATTTCGTTTTTTAGTCATAAAAAACCTGCCAGCGATGGCAGGtttttttgc                                                                                         <  1:1723607/100‑3 (MQ=255)
          aCGGAAAACGATCGTCGCCGCGCCAACCGCGCACCGAAAGACGATTAATTTCGTTTTTTAGTCATAAAAAACCTGCCATCGCTGGCAGGttttttatgac                                                                                       <  1:1175237/100‑6 (MQ=38)
          aCGGAAAACGATCGTCGCCGCGCCAACCGCGCACCGAAAGACGATTAATTTCGTTTTTTAGTCATAAAAAACCTGCCAGCGATGGCAGGttttttttgcc                                                                                       >  2:3778583/1‑97 (MQ=255)
          aCGGAAAACGATCGTCGCCGCGCCAACCGCGCACCGAAAGACGATTAATTTCGTTTTTTAGTCATAAAAAACCTGCCAGCGATGGCAGGttttttttgcc                                                                                       <  2:1093513/100‑4 (MQ=255)
          aCGGAAAACGATCGTCGCCGCGCCAACCGCGCACCGAAAGACGATTAATTTCGTTTTTTAGTCATAAAAAACCTGCCAGCGATGGCAGGttttttttgcc                                                                                       <  2:4010857/100‑4 (MQ=255)
           cGGAAAACGATCGTCGCCGCGCCAACCGCGCACCGAAAGACGATTAATTTCGTTTTTTAGTCATAAAAAACCTGCCAGCGATGGCAGGttttttttgcct                                                                                      <  1:374988/100‑5 (MQ=255)
           cGGAAAACGATCGTCGCCGCGCCAACCGCGCACCGAAAGACGATTAATTTCGTTTTTTAGTCATAAAAAACCTGCCAGCGATGGCAGGttttttttgcct                                                                                      <  2:3438404/100‑5 (MQ=255)
            ggAAAACGATCGTCGCCGCGCCAACCGCGCACCGAAAGACGATTAATTTCGTTTTTTAGTCATAAAAAACCTGCCAGCGATGGCAGG‑TTTTTTTTGCCTg                                                                                    >  2:2392444/1‑100 (MQ=255)
             gAAAACGATCGTCGCCGCGCCAACCGCGCACCGAAAGACGATTAATTTCGTTTTTTAGTCATAAAAAACCTGTCAGCGATGGCAGG‑TTTTTTTTGCCTGt                                                                                   <  1:1678880/100‑1 (MQ=255)
               aaaCGATCGTCGCCGCGCCAACCGCGCACCGAAAGACGATTAATTTCGTTTTTTAGTCATAAAAAACCTGCCAGCGATGGCAGGttttttttgccagatc                                                                                  >  2:1958616/1‑92 (MQ=255)
               aaaCGATCGTCGCCGCGCCAACCGCGCACCGAAAGACGATTAATTTCGTTTTTTAGTCATAAAAAACCTGCCAGCGATGGCAGG‑TTTTTTTTGCCTGTCg                                                                                 <  2:1011015/100‑1 (MQ=255)
                 aCGATCGTCGCCGCGCCAACCGCGCACCGAAAGACGATTAATTTCGTTTTTTAGTCATAAAAAACCTGCCAGCGATGGCAGG‑TTTTTTTTGCCTGTCGTg                                                                               <  2:728131/100‑1 (MQ=255)
                 aCGATCGTCGCCGCGCCAACCGCGCACCGAAAGACGATTAATTTCGTTTTTTAGTCATAAAAAACCTGCCAGCGATGGCAGG‑TTTTTTTTGCCTGTCGTg                                                                               <  2:516160/100‑1 (MQ=255)
                  cGATCGTCGCCGCGCCAACCGCGCACCGAAAGACGATTAATTTCGTTTTTTAGTCATAAAAAACCTGCCAGCGATGGCAGG‑TTTTTTTTGCCTGTCGTGa                                                                              >  2:411223/1‑100 (MQ=255)
                  cGATCGTCGCCGCGCCAACCGCGCACCGAAAGACGATTAATTTCGTTTTTTAGTCATAAAAAACCTGCCAGCGATGGCAGG‑TTTTTTTTGCCTGTCGTGa                                                                              <  1:2037346/100‑1 (MQ=255)
                    aTCGTCGCCGCGCCACCCGCGCACCGAAAGACGATTAATTTCGTTTTTTAGTCATAAAAACCCTGCCAGCGATGGCAGG‑TTTTTTTTGCCTGTCGTGAgc                                                                            <  1:3965370/100‑1 (MQ=255)
                    aTCGTCGCCGCGCCAACCGCGCACCGAAAGACGATTAATTTCGTTTTTTAGTCATAAAAAACCTGCCAGCGATGGCAGG‑TTTTTTTTGCCTGTCGTGa                                                                              <  2:1198064/98‑1 (MQ=255)
                    aTCGTCGCCGCGCCAACCGCGCACCGAAAGACGATTAATTTCGTTTTTTAGTCATAAAAAACCTGCCAGCGATGGCAGG‑TTTTTTTTGCCTGTCGTGAgc                                                                            <  2:24783/100‑1 (MQ=255)
                      cgtcgCCGCGCCAACCGCGCACCGAAAGACGATTAATTTCGTTTTTTAGTCATAAAAAACCTGCCAGCGATGGCAGG‑TTTTTTTTGCCTGTCGTGAgcgc                                                                          <  1:2249253/100‑1 (MQ=255)
                      cgtcgCCGCGCCAACCGCGCACCGAAAGACGATTAATTTCGTTTTTTAGTCATAAAAAACCTGCCAGCGATGGCAGG‑TTTTTTTTGCCTGTCGTGAgcgc                                                                          <  2:2096257/100‑1 (MQ=255)
                       gtcgCCGCGCCAACCGCGCACCGAAAGACGATTAATTTCGTCTTTTAGTCATAAAAAACCTGCCAGCGATGGCAGG‑TTTTTTTTGCCTGTCGTGAGCGca                                                                         <  1:3951306/100‑1 (MQ=255)
                         cgccgcGCCAACCGCGCACCGAAAGACGATTAATTTCGTTTTTTAGTCATAAAAAACCTGCCAGCGATGGCAGG‑TTTTTTTTGCCTGTCGTGAGCGcaca                                                                       >  2:276190/1‑100 (MQ=255)
                             gcgcCAACCGCGCACCGAAAGACGATTAATTTCGTTTTTTAGTCATAAAAAACCTGCCAGCGATGGCAGG‑TTTTTTTTGCCTGTCGTGAGCGCACACCaa                                                                   <  1:2873721/100‑1 (MQ=255)
                              cgcCAACCGCGCACCGAAAGACGATTAATTTCGTTTTTTAGTCATAAAAAACCTGCCAGCGATGGCAGG‑TTTTTTTTGCCTGTCGTGAGCGCACACCAAt                                                                  <  1:3778625/100‑1 (MQ=255)
                                   aCCGCGCACCGAAAGACGATTAATTTCGTTTTTTAGTCATAAAAAACCTGCCAGCGATGGCAGG‑TTTTTTTTGCCTGTCGTGAGCGCACACCAATGCGAg                                                             <  2:1000546/100‑1 (MQ=255)
                                   aCCGCGCACCGAAAGACGATTAATTTCGTTTTTTAGTCATAAAAAACCTGCCAGCGATGGCAGG‑TTTTTTTTGCCTGTCGTGAGCGCACACCAATGCGAg                                                             <  1:2267513/100‑1 (MQ=255)
                                       cgcACCGAAAGACGATTAATTTCGTTTTTTAGTCATAAAAAACCTGCCAGCGATGGCAGG‑TTTTTTTTGCCTGTCGTGAGCGCACACCAATGCGAGTAtc                                                         >  2:1486120/1‑100 (MQ=255)
                                       cgcACCGAAAGACGATTAATTTCGTTGTTTAGTCATAAAAAACCTGCCAGCGATGGCAGG‑TTTTTTTTGCCTGTCGTGAGCGCACACCAATGCGAGTAtc                                                         >  1:2553970/1‑100 (MQ=255)
                                         cACCGAAAGACGATTAATTTCGTTTTTTAGTCATAAAAAACCTGCCAGCGATGGCAGG‑TTTTTTTTGCCTGTCGTGAGCGCACACCAATGCGAGTAtctc                                                       >  1:1524649/1‑100 (MQ=255)
                                         cACCGAAAGACGATTAATTTCGTTTTTTAGTCATAAAAAACCTGCCAGCGATGGCAGG‑TTTTTTTTGCCTGTCGTGAGCGCACACCAATGCGAGTAtctc                                                       >  2:770353/1‑100 (MQ=255)
                                           ccGAAAGACGATTAATTTCGTTTTTTAGTCATAAAAAACCTGCCAGCGATGGCAGG‑TTTTTTTTGCCTGTCGTGAGCGCACACCAATGCGAGTATCTccc                                                     >  2:45642/1‑100 (MQ=255)
                                           ccGAAAGACGATTAATTTCGTTTTTTAGTCATAAAAAACCTGCCAGCGATGGCAGG‑TTTTTTTTGCCTGTCGTGAGCGCACACCAATGCGAGTATCTccc                                                     <  1:2936354/100‑1 (MQ=255)
                                            cGAAAGACGATTAATTTCGTTTTTTAGTCATAAAAAACCTGCCAGCGATGGCAGG‑TTTTTTTTGCCTGTCGTGAGCGCACACCAATGCGAGTATCTCCCt                                                    <  1:2757367/100‑1 (MQ=255)
                                              aaaGACGATTAATTTCGTTTTTTTTTTTTATTTAACCTGCCAGCGATGGCAGG‑TTTTTTTTGCCTGTCGTGAGCGCACACCAATGCGAGTATCTCCCtt                                                   >  2:3135336/1‑99 (MQ=255)
                                              aaaGACGATTAATTTCGTTTTTTAGTCATAAAAAACCTGCCAGCGATGGCAGG‑TTTTTTTTGCCTGTCGTGAGCGCACACCAATGCGAGTATCTCCCTta                                                  >  1:1878378/1‑100 (MQ=255)
                                              aaaGACGATTAATTTCGTTTTTTAGTCATAAAAAACCTGCCAGCGATGGCAGG‑TTTTTTTTGCCTGTCGTGAGCGCACACCAATGCGAGTATCTCCCTta                                                  >  1:3349470/1‑100 (MQ=255)
                                               aaGACGATTAATTTCGTTTTTTAGTCATAAAAAACCTGCCAGCGATGGCAGG‑TTTTTTTTGCCTGTCGTGAGCGCACACCAATGCGAGTATCTCCCTtat                                                 <  2:2033684/100‑1 (MQ=255)
                                                aGACGATTAATTTCGTTTTTTAGTCATAAAAAACCTGCCAGCGATGGCAGG‑TTTTTTTTGCCTGTCGTGAGCGCACACCAATGCGAGTATCTCCCTtata                                                <  2:3268007/100‑1 (MQ=255)
                                                  aCGATTAATTTCGTTTTTTAGTCATAAAAAACCTGCCAGCGATGGCAGG‑TTTTTTTTGCCTGTCGTGAGCGCACACCAATGCGAGTATCTCCCTTATAAg                                              >  1:1788697/1‑100 (MQ=255)
                                                   cGATTAATTTCGTTTTTTAGTCATAAAAAACCTGCCAGCGATGGCAGG‑TTTTTTTTGCCTGTCGTGAGCGCACACCAATGCGAGTATCTCCCTTATAAGt                                             >  1:3300467/1‑100 (MQ=255)
                                                    gATTAATTTCGTTTTTTAGTCATAAAAAACCTGCCAGCGATGGCAGG‑TTTTTTTTGCCTGTCGTGAGCGCACACCAATGCGAGTATCTCCCTTATAAGTc                                            <  2:3341047/100‑1 (MQ=255)
                                                     aTTAATTTCGTTTTTTTTTTTTTTTAAACCTGCCAGCGATGGCAGG‑TTTTTTTTGTCTGTCGTGAGTGCACACCAATGCGAGTATCTCCCTTATAATTCt                                           >  2:1739654/1‑100 (MQ=255)
                                                       tAATTTCGTTTTTTAGTCATAAAAAACCTGCCAGCGATGGCAGG‑TTTTTTTTGCCTGTCGTGAGCGCACACCAATGCGAGTATCTCCCTTATAAGTCtgt                                         >  1:2870603/1‑100 (MQ=255)
                                                        aaTTTCGTTTTTTAGTCATAAAAAACCTGCCAGCGATGGCAGG‑TTTTTTTTGCCTGTCGTGAGCGCACACCAATGCGAGTATCTCCCTTATAAGTCtgtg                                        <  1:2229538/100‑1 (MQ=255)
                                                        aaTTTCGTTTTTTAGTCATAAAAAACCTGCCAGCGATGGCAGG‑TTTTTTTTGCCTGTCGTGAGCGCACACCAATGCGAGTATCTCCCTTATAAGTCtgtg                                        >  1:4143138/1‑100 (MQ=255)
                                                        aaTTTCGTTTTTTAGTCATAAAAAACCTGCCAGCGATGGCAGG‑TTTTTTTTGCCTGTCGTGAGCGCACACCAATGCGAGTATCTCCCTTATAAGTCtgtg                                        <  2:251961/100‑1 (MQ=255)
                                                         aTTTCGTTTTTTAGTCATAAAAAACCTGCCAGCGATTGCAGG‑TTTTTTTTGCCTGTCGTGAGCGCACACCAATGCGAGTATCTCCCTTATAAGTCTGTgg                                       <  2:2383753/100‑1 (MQ=255)
                                                             cGTTTTTTAGTCATAAAAAACCTGCCAGCGATGGCAGG‑TTTTTTTTGCCTGTCGTGAGCGCACACCAATGCGAGTATCTCCCTTATAAGTCTGTGGTTAc                                   >  1:2779702/1‑100 (MQ=255)
                                                              gtgtgttATTCATTTAAAACCTGACAGCGATGGCAGG‑TTTTTTGTGCCTGTCGTGAGCGCACACCAATGCGAGTATCTCCCTTATAAGTCTGGGGTTAc                                   <  2:517757/94‑1 (MQ=255)
                                                                 ttttAGTCATAAAAAACCTGCCAGCGATGGCAGG‑TTTTTTTTGCCTGTCGTGAGCGCACACCAATGCGAGTATCTCCCTTATAAGTCTGTGGTTACGTCa                               <  1:2634203/100‑1 (MQ=255)
                                                                    tAGTCATAAAAAACCTGCCAGCGATGGCAGG‑TTTTTTTTGCCTGTCGTGAGCGCACACCAATGCGAGTATCTCCCTTATAAGTCTGTGGTTACGTCAATa                            <  1:2795994/100‑1 (MQ=255)
                                                                    tAGTCATAAAAAACCTGCCAGCGATGGCAGG‑TTTTTTTTGCCTGTCGTGAGCGCACACCAATGCGAGTATCTCCCTTATAAGTCTGTGGTTACGTCAATa                            <  1:1342347/100‑1 (MQ=255)
                                                                      ggcaaaaaaaaCCTGCCATCGCTGGCAGG‑TTTTTTATGACTGTCGTGAGCGCACACCAATGCGAGTATCTCCCTTATAAGTCTGTGGTTACGTCAATaga                          >  1:3684494/6‑100 (MQ=21)
                                                                      gTCATAAAAAACCTGCCAGCGATGGCAGG‑TTTTTTTTGCCTGTCGTGAGCGCACACCAATGCGAGTATCTCCCTTATAAGTCTGTGGTTACGTCAATaga                          >  1:3859145/1‑100 (MQ=255)
                                                                      gTCATAAAAAACCTGCCAGCGATGGCAGG‑TTTTTTTTGCCTGTCGTGAGCGCACACCAATGCGAGTATCTCCCTTATAAGTCTGTGGTTACGTCAATaga                          >  2:265118/1‑100 (MQ=255)
                                                                      gTCATAAAAAACCTGCCAGCGATGGCAGG‑TTTTTTTTGCCTGTCGTGAGCGCACACCAATGCGAGTATCTCCCTTATAAGTCTGTGGTTACGTCAATaga                          <  2:2779989/100‑1 (MQ=255)
                                                                       gcaaaaaaaaCCTGCCATCGCTGGCAGG‑TTTTTTTTGCCTGTCGTGAGCGCACACCAATGCGAGTATCTCCCTTATAAGTCTGTGGTTACGTCAATagag                         >  1:4096731/5‑100 (MQ=37)
                                                                       gcaaaaaaaaCCTGCCATCGCTGGCAGG‑TTTTTTTTGCCTGTCGTGAGCGCACACCAATGCGAGTATCTCCCTTATAAGTCTGTGGTTACGTCAATagag                         >  1:4096621/5‑100 (MQ=37)
                                                                        cATAAAAAACCTGCCAGCGATGGCAGG‑TTTTTTTTGCCTGTCGTGAGCGCACACCAATGCGAGTATCTCCCTTATAAGTCTGTGGTTACGTCAATagaga                        <  2:714890/100‑1 (MQ=255)
                                                                        cATAAAAAACCTGCCAGCGATGGCAGG‑TTTTTTTTGCCTGTCGTGAGCGCACACCAATGCGAGTATCTCCCTTATAAGTCTGTGGTTACGTCAATagaga                        >  1:3664250/1‑100 (MQ=255)
                                                                         aTAAAAAACCTGCCAGCGATGGCAGG‑TTTTTTTTGCCTGTCGTGAGCGCACACCAATGCGAGTATCTCCCTTATAAGTCTGTGGTTACGTCAATagagag                       >  1:181270/1‑100 (MQ=255)
                                                                          tAAAAAACCTGCCAGCGATGGCAGG‑TTTTTTTTGCCTGTCGTGAGCGCACACCAATGCGAGTATCTCCCTTATAAGTCTGTGGTTACGTCAATAGAGAGc                      >  2:48137/1‑100 (MQ=255)
                                                                           aaaaaaCCTGCCAGCGATGGCAGG‑TTTTTTTTGCCTGTCGTGAGCGCACACCAATGCGAGTATCTCCCTTATAAGTCTGTGGTTACGTCAATAGAGAGCt                     >  1:509742/1‑100 (MQ=255)
                                                                            aaaaaCCTGCCAGCGATGGCAGG‑TTTTTTTTGCCTGTCGTGAGCGCACACCAATGCGAGTATCTCCCTTATAAGTCTGTGGTTACGTCAATAGAGAGCtt                    >  1:3475508/1‑100 (MQ=255)
                                                                             aaaaCCTGCCATCGCTGGCAGG‑TTTTTTTTGCCTGTCGTGAGCGCACACCAATGCGAGTATCTCCCTTATAAGTCTGTGGTTACGTCAATAGAGAGCttt                   >  1:281310/1‑100 (MQ=38)
                                                                              aaaCCTGCCATCGCTGGCAGG‑TTTTTTTTGCCTGTCGTGAGCGCACACCAATGCGAGTATCTCCCTTATAAGTCTGTGGTTACGTCAATAGAGAGCTTTg                  <  2:4096578/100‑1 (MQ=39)
                                                                              aaaCCTGCCAGCGATGGCAGG‑TTTTTTTTGCCTGTCGTGAGCGCACACCAATGCGAGTATCTCCCTTATAAGTCTGTGGTTACGTCAATAGAGAGCTTTg                  <  2:1662693/100‑1 (MQ=255)
                                                                              aaaCCTGCCAGCGATGGCAGG‑TTTTTTTTGCCTGTCGTGAGCGCACACCAATGCGAGTATCTCCCTTATAAGTCTGTGGTTACGTCAATAGAGAGCTTTg                  >  2:1311566/1‑100 (MQ=255)
                                                                               aaCCTGCCATCGCTGGCAGG‑TTTTTTTTGCCTGTCGTGAGCGCACACCAATGCGAGTATCTCCCTTATAAGTCTGTGGTTACGTCAATAGAGAGCTTTGt                 >  1:1267284/1‑100 (MQ=39)
                                                                               aaCCTGCCAGCGATGGCAGG‑TTTTTTTTGCCTGTCGTGAGCGCACACCAATGCGAGTATCTCCCTTATAAGTCTGTGGTTACGTCAATAGAGAGCTTTGt                 <  1:2381092/100‑1 (MQ=255)
                                                                                nccTGCCATCGCTGGCAGG‑TTTTTTTTGCCTGTCGTGAGCGCACACCAATGCGAGTATCTCCCTTATAAGTCTGTGGTTACGTCAATAGAGAGCTTTGTc                >  2:4105856/2‑100 (MQ=255)
                                                                                aCCTGCCAGCGATGGCAGG‑TTTTTTTTGCCTGTCGTGAGCGCACACCAATGCGAGTATCTCCCTTATAAGTCTGTGGTTACGTCAATAGAGAGCTTTGTc                <  1:519439/100‑1 (MQ=255)
                                                                                  cTGCCAGCGATGGCAGG‑TTTTTTTTGCCTGTCGTGAGCGCACACCAATGCGAGTATCTCCCTTATAAGTCTGTGGTTACGTCAATAGAGAGCTTTGTcac              <  1:3693472/100‑1 (MQ=255)
                                                                                  cTGCCAGCGATGGCAGG‑TTTTTTTTGCCTGTCGTGAGCGCACACCAATGCGAGTATCTCCCTTATAAGTCTGTGGTTACGTCAATAGAGAGCTTTGTca               <  2:1159113/99‑1 (MQ=255)
                                                                                   tGCCAGCGATGGCAGG‑TTTTTTTTGCCTGTCGTGAGCGCACACCAATGCGAGTATCTCTCTTATAAGTCTGTGGTTACGGCAATAGAGAGCTTTTTcaca             >  2:2084694/1‑100 (MQ=255)
                                                                                   tGCCAGCGATGGCAGG‑TTTTTTTTGCCTGTCGTGAGCGCACACCAATGCGAGTATCTCCCTTATAAGTCTGTGGTTACGTCAATAGAGAGCTTTGTcaca             >  1:1170991/1‑100 (MQ=255)
                                                                                   tGCCAGCGATGGCAGG‑TTTTTTTTGCCTGTCGTGAGCGCACACCAATGCGAGTATCTCCCTTATAAGTCTGTGGTTACGTCAATAGAGAGCTTTGTcaca             >  1:2372901/1‑100 (MQ=255)
                                                                                   tGCCAGCGATGGCAGG‑TTTTTTTTGCCTGTCGTGAGCGCACACCAATGCGAGTATCTCCCTTATAAGTCTGTGGTTACGTCAATAGAGAGCTTTGTcaca             >  2:3018309/1‑100 (MQ=255)
                                                                                     ccAGCGATGGCAGG‑TTTTTTTTGCCTGTCGTGAGCGCACACCAATGCGAGTATCTCCCTTATAAGTCTGTGGTTACGTCAATAGAGAGCTTTGTCACAAt           <  2:1063/100‑1 (MQ=255)
                                                                                       aGCGATGGCAGG‑TTTTTTTTGCCTGTCGTGAGCGCACACCAATGCGAGTATCTCCCTTATAAGTCTGTGGTTACGTCAATAGAGAGCTTTGTCACAATTa         <  1:726771/100‑1 (MQ=255)
                                                                                         cGATGGCAGG‑TTTTTTTTGCCTGTCGTGAGCGCACACCAATGCGAGTATCTCCCTTATAAGTCTGTGGTTACGTCAATAGAGAGCTTTGTCACAATTATc       <  2:967233/100‑1 (MQ=255)
                                                                                              gCAGG‑TTTTTTTTGCCTGTCGTGAGCGCACACCAATGCGAGTATCTCCCTTATAAGTCTGTGGTTACGTCAATAGAGAGCTTTGTCACAATTATCTGCaa  >  1:2906247/1‑100 (MQ=255)
                                                                                              gCAGG‑TTTTTTTTGCCTGTCGTGAACGCAGACCAATGCGAGTATGTCCCTTATAAGTCTGTGGTTACGTAAATAGAGAGCTTTGTCACAATTGTCt      >  2:2301077/1‑96 (MQ=255)
                                                                                                   |                                                                                               
ACGTCACCTGACGGAAAACGATCGTCGCCGCGCCAACCGCGCACCGAAAGACGATTAATTTCGTTTTTTAGTCATAAAAAACCTGCCAGCGATGGCAGGTTTTTTTTTGCCTGTCGTGAGCGCACACCAATGCGAGTATCTCCCTTATAAGTCTGTGGTTACGTCAATAGAGAGCTTTGTCACAATTATCTGCAA  >  NC_000913/4060173‑4060367

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A