Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A10 F169 I0 R1 76 156.9 7610945 95.9% 7298896 99.8

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 3,304,813 (C)7→6 100% coding (1005/1245 nt) mtr ← tryptophan transporter of high affinity

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,304,8070C.97.9% 638.2 / 3.6 141coding (1011/1245 nt)mtrtryptophan transporter of high affinity
Reads supporting (aligned to +/- strand):  ref base C (1/1);  new base . (67/71);  total (69/72)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.94e-01

Only 100 of 159 total aligned reads displayed.

CGATGCACGGGCTAACAGCGCCGGAACAATTGCCGCCCAGATGGTAGCCGCTAAACCAGCATAACCAATGGCGTACAGGAATCCGTTCGGGAACAACAGCCCCCCCACAACTGGCGGGGCAAAGGTCAGCAATGCCGTTTTCAAGCGGCCCACAGCCGAGTCGTCGAAACCAAACAGATCTGCCAGATAGTCA  >  NC_000913/3304708‑3304900
                                                                                                   |                                                                                             
cgATGCACTGGCTAACAGCGACGGAACAATTGCCGCCCAGATGGTAGCCGCTAAACCAGCAGAACCAAGGGCGTACAGGAATCCGTTCGGGAACAACAGc                                                                                               <  1:2049332/100‑1 (MQ=255)
 gatacACGGGCTAACAGCGCCGGAACAATTGCCGCCCAGATGGTAGCCGCTAAACCAGCATAACCAATGGCGTACAGGAATCCGTTCGGGAACAACAGcc                                                                                              <  1:3357763/96‑1 (MQ=255)
  aTGCACGGGCTAACAGCGCCGGAACAATTGCCGCCCAGATGGTAGCCGCTAAACCAGCATAACCAATGGCGTACAGGAATCCGTTCGGGAACAACAGccc                                                                                             >  1:3586980/1‑100 (MQ=255)
   tGCACGGGCTAACAGCGCCGGAACAATTGCCGCCCAGATGGTAGCCGCTAAACCAGCATAACCAATGGCGTACAGGAATCCGTTCGGGAACAACAGcccc                                                                                            <  2:297630/100‑1 (MQ=255)
    gCACGGGTTAACAGCGCCGGAACAATTGCCGCCCAGATGGTAGCCGCTAAACCAGCATAACCAATCGCGTACAGGAATCCGTTCGGGAACAACAGccccc                                                                                           >  1:1988560/1‑100 (MQ=255)
    gCACGGGCTAACAGCGCCGGAACAATTGCCGCCCAGATGGTAGCCGCTAAACCAGCATAACCAATGGCGTACAGGAATCCGTTCGGGAACAACAGccccc                                                                                           <  1:1323267/100‑1 (MQ=255)
     cACGGGCTAACAGCGCCGGAACAATTGCCGCCCAGATGGTAGCCGCTAAACCAGCATAACCAATGGCGTACAGGAATCCGTTCGGGAACAACAGcccccc                                                                                          >  2:37698/1‑100 (MQ=255)
     cACGGGCTAACAGCGCCGGAACAATTGCCGCCCAGATGGTAGCCGCTAAACCAGCATAACCAATGGCGTACAGGAATCCGTTCGGGAACAACAGccccc                                                                                           >  1:1749663/1‑99 (MQ=255)
      aCGGGCTAACAGCGCCGGAACAATTGCCGCCCAGATGGTAGCCGCTAAACCAGCATAACCAATGGCGTACAGGAATCCGTTCGGGAACAACAGcccccc                                                                                          <  1:221363/99‑1 (MQ=255)
       cGGGCTAACAGCGCCGGAACAATTGCCGCCCAGATGGTAGCCGCTAAACCAGCATAACCAATGGCGTACAGGAATCCGTTCGGGAACAACAGccccccac                                                                                        <  1:263365/100‑3 (MQ=255)
       cGGGCTAACAGCGCCGGAACAATTGCCGCCCAGATGGTAGCCGCTAAACCAGCATAACCAATGGCGTACAGGAATCCGTTCGGGAACAACAGccccccac                                                                                        >  1:1794190/1‑98 (MQ=255)
         ggCTAACAGCGCCGGAACAATTGCCGCCCAGATGGTAGCCGCTAAACCAGCATAACCAATGGCGTACAGGAATCCGTTCGGGAACAACAGccccc                                                                                           >  1:363008/1‑95 (MQ=255)
          gCTAACAGCGCCGGAACAATTGCCGCCCAGATGGTAGCCGCTAAACCAGCATAACCAATGGCGTACAGGAATCCGTTCGGGAACAACAG‑CCCCCCACAAc                                                                                    <  2:708531/100‑1 (MQ=255)
            tAACAGCGCCGGAACAATTGCCGCCCAGATGGTAGCCGCTAAACCAGCATAACCAATGGCGTACAGGAATCCGTTCGGGAACAACAG‑CCCCCCACAACTg                                                                                  <  1:2851014/100‑1 (MQ=255)
               cAGCGCCGGAACAATTGCCGCCCAGATGGTAGCCGCTAAACCAGCATAACCAATGGCGTACAGGAATCCGTTCGGGAACAACAG‑CCCCCCACAACTGGCg                                                                               >  2:3106890/1‑100 (MQ=255)
                aGCGCCGGAACAATTGCCGCCCAGATGGTAGCCGCTAAACCAGCATAACCAATGGCGTACAGGAATCCGTTCGGGAACAACAG‑CCCCCCACAACTGGCgg                                                                              <  2:2733493/100‑1 (MQ=255)
                aGCGCCGGAACAATTGCCGCCCAGATGGTAGCCGCTAAACCAGCATAACCAATGGCGTACAGGAATCCGTTCGGGAACAACAG‑CCCCCCACAACTGGCgg                                                                              <  2:604032/100‑1 (MQ=255)
                aGCGCCGGAACAATTGCCGCCCAGATGGTAGCCGCTAAACCAGCATAACCAATGGCGTACAGGAATCCGTTCGGGAACAACAG‑CCCCCCACAACTGGCgg                                                                              <  1:259716/100‑1 (MQ=255)
                aGCGCCGGAACAATTGCCGCCCAGATGGTAGCCGCTAAACCAGCATAACCAATGGCGTACAGGAAGCCGTTCGGGAACAACAG‑CCCCCCACAACTGGCgg                                                                              >  1:2359886/1‑100 (MQ=255)
                 gcgcCGGAACAATTGCCGCCCAGATGGTAGCCGCTAAACCAGCATAACCAATGGCGTACAGGAATCCGTTCGGGAACAACAG‑CCCCCCACAACTGGCggg                                                                             >  2:533725/1‑100 (MQ=255)
                  cgcCGGAACAATTGCCGCCCAGATGGTAGCCGCTAAACCAGCATAACCAATGGCGTACAGGAATCCGTTCGGGAACAACAG‑CCCCCCACAACTGGCggg                                                                             <  1:2137269/99‑1 (MQ=255)
                   gcCGGAACAATTGCCGCCCAGATGGTAGCCGCTAAACCAGCATAACCAATGGCGTACAGGAATCCGTTCGGGAACAACAG‑CCCCCCACAACTGGCGGGGc                                                                           <  1:1535057/100‑1 (MQ=255)
                    ccGGAACAATTGCCGACCATATGGTAACCGCTAAACCACCATAACCAAAGGCGTACAGGAAGCCGTTCGGGAACAACAG‑CCCCCCACAACTGGCGGGGc                                                                           <  1:2663516/99‑1 (MQ=255)
                     cGGAACAATTGCCGCCCAGATGGTAGCCGCTAAACCAGCATAACCAATGGCGTACAGGAATCCGTTCGGGAACAACAG‑CCCCCCACAACTGGCGGGGCaa                                                                         >  1:283934/1‑100 (MQ=255)
                     cGGAACAATTGCCGCCCAGATGGTAGCCGCTAAACCAGCATAACCAATGGCGTACAGGAATCCGTTCGGGAACAACAG‑CCCCCCACAACTGGCGGGGCaa                                                                         >  1:980012/1‑100 (MQ=255)
                     cGGAACAATTGCCGCCCAGATGGTAGACGCTAAACCAGCATAACCAATGGCGTACAGGAATCCGTTCGGGAACAACAG‑CCCCCCACAACTGGCGGGGCaa                                                                         >  1:3522141/1‑100 (MQ=255)
                      ggcaCAATTGCCGCCCAGATGGTAGCCGCTAAGACAGAATAACCAATGGCGTACAGGCATCCGATCGGGAACAACAG‑CCCCCCACAACTGGCGGGGcta                                                                         >  1:1649696/4‑97 (MQ=255)
                      ggAACAATTGCCGCCCAGATGGTAGCCGCTAAACCAGCATAACCAATGGCGTACAGGAATCCGTTCGGGAACAACAG‑CCCCCCACAACTGGCGGGGCaaa                                                                        >  2:539768/1‑100 (MQ=255)
                      ggAACAATAGCCGCCGAGATGGTAGCCGCTAAACCAGCCAAACCCATGGCCTCCAGGACTCCGTTTCAGAACAACACACCCCACACaatgaccgggc                                                                            >  1:3485417/1‑88 (MQ=255)
                       gAACAATTGCCGCCCAGATGGTAGCCGCTAAACCAGCATAACCAATGGCGTACAGGAATCCGTTCGGGAACAACAG‑CCCCCCACAACTGGCGGGGCAAAg                                                                       >  2:3442407/1‑100 (MQ=255)
                       gAACAATTGCCGCCCAGATGGTAGCCGCTAAACCAGCATAACCAATGGCGTACAGGAATCCGTTCGGGAACAACAG‑CCCCCCACAACTGGCGGGGCAAAg                                                                       <  1:1107894/100‑1 (MQ=255)
                       gAAAAATGGCCGCCGAGTTGGTAGCCGCTAAACCAGCATAACAACTGGCGTACAGGAATCCGTCCGTTAACAACAG‑CCCCCCACGACTGGCGGTGCAAAg                                                                       <  1:2674479/100‑1 (MQ=255)
                        aaCAAGTGCCGCCCAGATGGTAGCCGCTAAACCAGCATAACCAATGGCGTACAGGAATCCGTTCGGGAACAACAG‑CCCCCCACAACTGGCGGGGCAAAgg                                                                      <  1:3433309/100‑1 (MQ=255)
                          cAATTGCCGCCCAGATGGTAGCCGCTAAACCAGCATAACCAATGGCGTACAGGAATCCGTTCGGGAACAACAG‑CCCCCCACAACTGGCGGGGCAAAGGTc                                                                    <  1:2751794/100‑1 (MQ=255)
                          cAATTGCCGCCCAGATGGTAGCCGCTAAACCAGCATAACCAATGGCGTACAGGAATCCGTTCGGGAACAACAG‑CCCCCCACAACTGGCGGGGCAAAGGTc                                                                    >  2:502942/1‑100 (MQ=255)
                          cAATTGCCGCCCAGATGGTAGCCGCTAAACCAGCATAACCAATGGCGTACAGGAATCCGTTCGGGAACAACAG‑CCCCCCACAACTGGCGGGGCAAAGGTc                                                                    >  2:3327553/1‑100 (MQ=255)
                          cAATTGCCGCCCAGATGGTAGCCGCTAAACCAGCATAACCAATGGCGTACAGGAATCCGTTCGGGAACAACAG‑CCCCCCACAACTGGCGGGGCAAAGGTc                                                                    >  2:3431078/1‑100 (MQ=255)
                           aaTTGCCGCCCAGATGGTAGCCGCTAAACCAGCATAACCAATGGCGTACAGGAATCCGTTCGGGAACAACAG‑CCCCCCACAACTGGCGGGGCAAAGGTCa                                                                   <  2:1897129/100‑1 (MQ=255)
                            aTTGCCGCCCAGATGGTAGCCGCTAAACCAGCATAACCAATGGCGTACAGGAATCCGTTCGGGAACAACAG‑CCCCCCACAACTGGCGGGGCAAAGGTcgg                                                                  <  1:1474439/100‑3 (MQ=255)
                            aTTGCCGCCCAGATGGTAGCCGCTAAACCAGCATAACCAATGGCGTACAGGAATCCGTTCGGGAACAACAG‑CCCCCCACAACTGGCGGGGCAAAGGTCAg                                                                  >  1:785854/1‑100 (MQ=255)
                             ttGCCGCCCAGATGGTAGCCGCTAAACCAGCATAACCAATGGCGTACAGGAATCCGTTCGGGAACAACAG‑CCCCCCACAACTGGCGGGGCAAAGGTCAGc                                                                 <  2:3639259/100‑1 (MQ=255)
                             ttGCCGCCCAGATGGTAGCCGCTAAACCAGCATAACCAATGGCGTACAGGAATCCGTTCGGGAACAACAG‑CCCCCCACAACTGGCGGGGCAAAGGTCAGc                                                                 <  2:363010/100‑1 (MQ=255)
                              tGCCGCCCAGATGGTAGCCGCTAAACCAGCATAACCAATGGCGTACAGGAATCCGTTCGGGAACAACAG‑CCCCCCACAACTGGCGGGGCAAAGGTCAGCa                                                                <  2:2409565/100‑1 (MQ=255)
                               gccgccCAGATGGTAGCCGCTAAACCAGCATAACCAATGGCGTCCAGGAATCCGTTCGGGAACAACAG‑CCCCCCACAACTGGCGCCGCAAAGTTCAGc                                                                 >  1:568136/1‑98 (MQ=255)
                                ccgccCAGATGGTAGCCGCTAAACCAGCATAACCAATGGCGTACAGGAATCCGTTCGGGAACAACAG‑CCCCCCACAACTGGCGGGGCAAAGGTCAGCAAt                                                              <  2:1826392/100‑1 (MQ=255)
                                  gccCAGATGGTAGCCGCTAAACCAGCATAACCAATGGCGTACAGGAATCCGTTCGGGAACAACAG‑CCCCCCACAACTGGCGGGGCAAAGGTCAGCAATGc                                                            >  1:3135415/1‑100 (MQ=255)
                                  gccCAGATGGTAGCCGCTAAACCAGCATAACCAATGGCGTACAGGAATCCGTTCGGGAACAACAG‑CCCCCCACAACTGGCGGGGCAAAGGTCAGCAATGc                                                            <  2:2588792/100‑1 (MQ=255)
                                  gccCAGATGGTAGCCGCTAAACCAGCATAACCAATGGCGTACAGGAATCCGTTCGGGAACAACAG‑CCCCCAACAAATGGCGGGGCAAAGGTCAGCaa                                                               >  1:1044322/1‑97 (MQ=255)
                                    ccAGATGGTAGCCGCTAAACCAGCATAACCAATGGCGTACAGGAATCCGTTCGGGAACAACAG‑CCCCCCACAACTGGCGGGGCAAAGGTCAGCAATGCCg                                                          >  1:3037664/1‑100 (MQ=255)
                                          ggTAGCCGCTAAACCAGCATAACCAATGGCGTACAGGAATCCGTTCGGGAACAACAG‑CCCCCCACAACTGGCGGGGCAAAGGTCAGCAATGCCGTTTTCa                                                    <  2:2449072/100‑1 (MQ=255)
                                          ggTAGCCGCTAAACCAGCATAACCAATGGCGTACAGGAATCCGTTCGGGAACAACAG‑CCCCCCACAACTGGCGGGGCAAAGGTCAGCAATGCCGCTTTCa                                                    >  1:295310/1‑100 (MQ=255)
                                           gTAGCCGCTAAACCAGCATAACCAATGGCGTACAGGAATCCGTTCGGGAACAACAG‑CCCCCCACAACTGGCGGGGCAAAGGTCAGCACTGCCGTTTTCaa                                                   >  1:2398575/1‑100 (MQ=255)
                                           gTAGCCGCTAAACCAGCATAACCAATGGCGTACAGGAATCCGTTCGGGAACAACAG‑CCCCCCACAACTGGCGGGGCAAAGGTCAGCAATGCCGTTTTCaa                                                   >  2:3247949/1‑100 (MQ=255)
                                            tAGCCGCTAAACCAGCATAACCAATGGCGTACAGGAATCCGTTCGGGAACAACAG‑CCCCCCACAACTGGCGGGGCAAAGGTCAGCAATGCCGTTTTCAAg                                                  <  2:3574158/100‑1 (MQ=255)
                                             aGCCGCTAAACCAGCATAACCAATGGCGTACAGGAATCCGTTCGGGAACAACAG‑CCCCCCACAACTGGCGGGGCAAAGGTCAGCAATGCCGTTTTCAAGc                                                 >  2:1323239/1‑100 (MQ=255)
                                                cGCTAAACCAGCATAACCAATGGCGTACAGGAATCCGTTCGGGAACAACAG‑CCCCCCACAACTGGCGGGGCAAAGGTCAGCAATGCCGTTTTCAAGCGGc                                              <  2:2695128/100‑1 (MQ=255)
                                                cGCTAAACCAGCATAACCAATGGCGTACAGGAATCCGTTCGGGAACAACAG‑CCCCCCACAACTGGCGGGGCAAAGGTCAGCAATGCCGTTTTCAAGCGGc                                              >  2:897424/1‑100 (MQ=255)
                                                cGCTAAACCAGCATAACCAATGGCGTACAGGAATCAGTTCGGGAACAACAG‑CCCCCCACAACTGGCGGGGCAAAGGTCAGCAATGCCGTTTTCAAGCGGc                                              >  1:668700/1‑100 (MQ=255)
                                                    aaaCCAGCATAACCAATGGCGTACAGGAATCCGTTCGGGAACAACAG‑CCCCCCACAACTGGCGGGGCAAAGGTCAGCAATGCCGTTTTCAAGCGGCCcac                                          >  1:904564/1‑100 (MQ=255)
                                                    aaaCCAGCATAACCAATGGCGTACAGGAATCCGTTCGGGAACAACAG‑CCCCCCACAACTGGCGGGGCAAAGGTCAGCAATGCCGTTTTCAAGCGGCCcac                                          <  1:1137861/100‑1 (MQ=255)
                                                    aaaCCAGCATAACCAATGGCGTACAGGAATCCGTTCGGGAACAACAG‑CCCCCCACAACTGGCGGGGCAAAGGTCAGCAATGCCGTTTTCAAGCGGCCcac                                          >  1:2501911/1‑100 (MQ=255)
                                                       ccAGCATAACCAATGGCGTACAGGAATCCGTTCGGGAACAACAG‑CCCCCCACAACTGGCGGGGCAAAGGTCAGCAATGCCGTTTTCAAGCGGCCCACAGc                                       >  2:1803132/1‑100 (MQ=255)
                                                        cAGCATAACCAATTGCGTACAGGAATCCGTTGGGGACCAACAG‑CCCCCCACAACTGGCGGGGCAAAGGGTAGCCATGCCGCTTGAAAACGGcatttc                                         <  1:828853/97‑6 (MQ=255)
                                                         aGCATAACCAATGGCGTACAGGAATCCGTTCGGGAACAACAG‑CCCCCCACAACTGGCGGGGCAAAGGTCAGCAATGCCGTTTTCAAGCGGCCCACAGCCg                                     <  2:3178379/100‑1 (MQ=255)
                                                          gCATAACCAATGGCGTACAGGAATCCGTTCGGGAACAACAG‑CCCCCCACAACTGGCGGGGCAAAGGTCAGCAATGCCGTTTTCAAGCGGCCCACAGCCGa                                    >  2:1936788/1‑100 (MQ=255)
                                                           cagaaCCAATGGCGTACAGGAATCCCTTCGGGAACAACAG‑CCCCCCACAACTCGCGGGGCAAAGGTCAGCAATGCCGTTTTCAAGCGGCCCACAGCCGAg                                   <  1:2058431/97‑1 (MQ=255)
                                                           cATAACCAATGGCGTACAGGAATCCGTTCGGGAACAACAG‑CCCCCCACAACTGGCGGGGCAAAGGTCAGCAATGCCGTTTTCAAGCGGCCCACAGCCGAg                                   <  2:1418378/100‑1 (MQ=255)
                                                           cATAACCAATGGCGTACAGGAATCCGTTCGGGAACAACAG‑CCCCCCACAACTGGCGGGGCAAAGGTCAGCAATGCCGTTTTCAAGCGGCCCACAGCCGAg                                   >  2:1428281/1‑100 (MQ=255)
                                                           cATAACCAATGGCGTACAGGAATCCGTTCGGGAACAACAG‑CCCCCCACAACTGGCGGGGCAAAGGTCAGCAATGCCGTTTTCAAGCGGCCCACAGCCGAg                                   <  1:3702252/100‑1 (MQ=255)
                                                             tAACCAATGGCGTACAGGAATCCGTTCGGGAACAACAG‑CCCCCCACAACTGGCGGGGCAAAGGTCAGCAATGCCGTTTTCAAGCGGCCCACAGCCGAgtc                                 <  1:980007/100‑1 (MQ=255)
                                                               aCCAATGGCGTACAGGAATCCGTTCGGGAACAACAG‑CCCCCCACAACTGGCGGGGCAAAGGTCAGCAATGCCGTTTTCAAGCGGCCCACAGCCGAgtcgt                               >  1:730924/1‑100 (MQ=255)
                                                                ccAATGGCGTACAGGAATCCGTTCGGGAACAACAG‑CCCCCCACAACTGGCGGGGCAAAGGTCAGCAATGCCGTTTTCAAGCGGCCCACAGCCGAgtcgtc                              >  1:3651104/1‑100 (MQ=255)
                                                                 cAATGGCGTATAGGAATCCGTTCGGGAACAACAG‑CCCCCCACAACTGGCGGGGCAAAGGTCAGCAATGCCGTTTTCAAGCGGCCCACAGCCGAGtcgtcg                             >  1:1190816/1‑100 (MQ=255)
                                                                   aTGGCGTACAGGAATCCGTTCGGGAACAACAG‑CCCCCCACAACTGGCGGGGCAAAGGTCAGCAATGCCGTTTTCAAGCGGCCCACAGCCGAGTCGTCGaa                           <  1:373412/100‑1 (MQ=255)
                                                                    tGGCGTACAGGAATCCGTTCGGGAACAACAG‑CCCCCCACAACTGGCGGGGCAAAGGTCAGCAATGCCGTTTTCAAGCGGCCCACAGCCGAGTCGTCGaaa                          <  2:2462986/100‑1 (MQ=255)
                                                                    tGGCGTACAGGAATCCGTTCGGGAACAACAG‑CCCCCCACAACTGGCGGGGCAAAGGTCAGCAATGCCGTTTTCAAGCGGCCCACAGCCGAGTCGTCGaaa                          <  2:2187545/100‑1 (MQ=255)
                                                                    tGGCGTACAGGAATCCGTTCGGGAACAACAG‑CCCCCCACAACTGGCGGGGCAAAGGTCAGCAATGCCGTTTTCAAGCGGCCCACAGCCGAGTCGTCGaaa                          <  2:13144/100‑1 (MQ=255)
                                                                    tGGCGTACAGGAATCCGTTCGGGAACAACAG‑CCCCCCACAACTGGCGGGGCAAAGGTCAGCAATGCCGTTTTCAAGCGGCCCACAGCCGAGTCGTCGaaa                          <  1:2613022/100‑1 (MQ=255)
                                                                     gccGTACAGGAATCCGTTCGGGAACAACAG‑CCCCCCACAACTAGCGGGGCAAAGGTCAGCAATGCCGTTTTCAAGCGGCCCACAGCCGAGTCGTCGAAAc                         >  2:2570443/3‑100 (MQ=255)
                                                                       cGTACAGGAATCCGTTCGGGAACAACAG‑CCCCCCACAACTGGCGGGGCAAAGGTCAGCAATGCCGTTTTCAAGCGGCCCACAGCCGAGTCGTCGAAACCa                       >  2:3309850/1‑100 (MQ=255)
                                                                         tACAGGAATCCGTTCGGGAACAACAG‑CCCCCCACAACTGGCGGGGCAAAGGTCAGCAATGCCGTTTTCAAGCGGCCCACAGCCGAGTCGTCGAAACCaaa                     >  1:3283929/1‑100 (MQ=255)
                                                                         tACAGGAATCCGTTCGGGAACAACAG‑CCCCCCACAACTGGCGGGGCAAAGGTCAGCAATGCCGTTTTCAAGCGGCCCACAGCCGAGTCGTCGAAACCaaa                     >  2:3190578/1‑100 (MQ=255)
                                                                         tACAGGAATCCGTTCGGGAACAACAG‑CCCCCCACAACTGGCGGGGCAAAGGTCAGCAATGCCGTTTTCAAGCGGCCCACAGCCGAGTCGTCGAAACCaaa                     <  2:3343415/100‑1 (MQ=255)
                                                                          aCAGGAATCCGTTCGGGAACAACAG‑CCCCCCACAACTGGCGGGGCAAAGGTCAGCAATGCCGTTTTCAAGCGGCCCACAGCCGAGTCGTCGAAACCAAAc                    <  2:1629094/100‑1 (MQ=255)
                                                                           cAGGAATCCGTTCGGGAACAACAG‑CCCCCCACAACTGGCGGGGCAAAGGTCAGCAATGCCGTTTTCAAGCGGCCCACAGCCGAGTCGTCGAAACCAAACa                   <  2:3135435/100‑1 (MQ=255)
                                                                           cAGGAATCCGTTCGGGAACAACAG‑CCCCCCACAACTGGCGGGGCAAAGGTCAGCAATGCCGTTTTCAAGCGGCCCACAGCCGAGTCGTCGAAACCAAACa                   <  1:504929/100‑1 (MQ=255)
                                                                             ggAATCCGTTCGGGAACAACAG‑CCCCCCACAACTGGCGGGGCAAAGGTCAGCAATGCCGTTTTCAAGCGGCCCACAGCCGAGTCGTCGAAACCAAACAGa                 >  2:3803766/1‑100 (MQ=255)
                                                                               aaTCCGTTCGGGAACAACAG‑CCCCCCACAACTGGCGGGGCAAAGGTCAGCAATGCCGTTTTCAAGCGGCCCACAGCCGAGTCGTCGAAACCAAACAGATc               <  1:3190555/100‑1 (MQ=255)
                                                                                 tCCGTTCGGGAACAACAG‑CCCCCCACAACTGGCGGGGCAAAGGTCAGCAATGCCGTTTTCAAGCGGCCCACAGCCGAGTCGTCGAAACCAAACAGATCTg             <  2:3263199/100‑1 (MQ=255)
                                                                                  ccGTTCGGGAACAACAG‑CCCCCCACAACTGGCGGGTCAAAGGTCAGCAATGCCGTTTTCAAGCGGGCCACAGCCGCGTCGTCGAAAACAAACAGctct              >  1:1585848/1‑98 (MQ=255)
                                                                                   cGTTCGGGAACAACAG‑CCCCCCACAACTGGCGGGGCAAAGGTCAGCAATGCCGTTTTCAAGCGGCCCACAGCCGAGTCGTCGAAACCAAACAGATCTGcc           >  1:2647535/1‑100 (MQ=255)
                                                                                   cGTTCGGGAACAACAG‑CCCCCCACAACTGGCGGGGCAAAGGTCAGCAATGCCGTTTTCAAGCGGCCCACAGCCGAGTCGTCGAAACCAAACAGATCTGcc           >  2:2061762/1‑100 (MQ=255)
                                                                                    gttatagAACAACAG‑ACCCCCTCCACTGGCGGGGCAAAGGTCAGCAATGCCGTTTTCAAGCGGCCCACAACCTAGTCGTCGAAACCAAACATATCAGCCa          <  1:823610/94‑1 (MQ=255)
                                                                                        gggAACAACAG‑CCCCCCACAACTGGCGGGGCAAAGGTCAGCAATGCCGTTTTCAAGCGGCCCACAGCCGAGTCGTCGAAACCAAACAGCTCTGCCAg         >  1:1384447/1‑97 (MQ=255)
                                                                                        gggAACAACAG‑CCCCCCACAACTGGCGGGGCAAAGGTCAGCAATGCCGTTTTCAAGCGGCCCACAGCCGAGTCGTCGAAACCAAACAGCTCTGCCAGATa      >  1:1151164/1‑100 (MQ=255)
                                                                                        gggAACAACAG‑CCCCCCACAACTGGCGGGGCAAAGGTCAGCAATGCCGTTTTCAAGCGGCCCACAGCCGAGTCGTCGAAACCAAACAGATCTGCCAGATa      >  1:1777588/1‑100 (MQ=255)
                                                                                        gggAACAACAG‑CCCCCCACAACTGGCGGGGCAAAGGTCAGCAATGCCGTTTTCAAGCGGCCCACAGCCGAGCCGTCGAAACCAACCAGATCTGCCAg         >  1:1076560/1‑97 (MQ=255)
                                                                                         ggAACAACAG‑CCCCCCACAACTGGCGGGGCAAAGGTCAGCAATGCCGTTTTCAAGCGGCCCACAGCCGAGTCGTCGAAACCAAACAGATCTGCCAGATAg     >  1:310996/1‑100 (MQ=255)
                                                                                            acaacaG‑CCCCCCACAACTGGCGGGGCAAAGGTCAGCAATGCCGTTTTCAAGCGGCCCACAGCCGAGTCGTCGAAACCAAACAGATCTGCCAGATCGTca  >  1:1279649/1‑100 (MQ=255)
                                                                                            acaacaG‑CCCCCCACAACTGGCGGGGCAAAGGTCAGCAATGCCGTTTTCAAGCGGCCCACAGCCGAGTCGTCGAAACCAAACAGATCTGCCAGATAGTca  <  2:21313/100‑1 (MQ=255)
                                                                                                   |                                                                                             
CGATGCACGGGCTAACAGCGCCGGAACAATTGCCGCCCAGATGGTAGCCGCTAAACCAGCATAACCAATGGCGTACAGGAATCCGTTCGGGAACAACAGCCCCCCCACAACTGGCGGGGCAAAGGTCAGCAATGCCGTTTTCAAGCGGCCCACAGCCGAGTCGTCGAAACCAAACAGATCTGCCAGATAGTCA  >  NC_000913/3304708‑3304900

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A