Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A10 F169 I1 R1 59 169.8 8365175 96.8% 8097489 99.9

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 3,304,813 (C)7→6 coding (1005/1245 nt) mtr ← tryptophan transporter of high affinity

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,304,8070C.99.3% 644.4 / ‑1.3 142coding (1011/1245 nt)mtrtryptophan transporter of high affinity
Reads supporting (aligned to +/- strand):  ref base C (1/0);  new base . (68/72);  total (70/72)
Fisher's exact test for biased strand distribution p-value = 4.89e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.77e-01

Only 100 of 162 total aligned reads displayed.

CGATGCACGGGCTAACAGCGCCGGAACAATTGCCGCCCAGATGGTAGCCGCTAAACCAGCATAACCAATGGCGTACAGGAATCCGTTCGGGAACAACAGCCCCCCCACAACTGGCGGGGCAAAGGTCAGCAATGCCGTTTTCAAGCGGCCCACAGCCGAGTCGTCGAAACCAAACAGATCTGCCAGATAGTCAA  >  NC_000913/3304708‑3304901
                                                                                                   |                                                                                              
cgATGCACGGGCTAACAGCGCCGGAACAATTGCCGCCCAGATGGTAGCCGCTAAACCAGCATAACCAATGGCGTACAGGAATCCGTTCGGGAACAACAGc                                                                                                <  1:1334014/100‑1 (MQ=255)
 gATGCACGGGCTAACAGCGCCGGAACAATTGCCGCCCAGATGGTAGCCGCTAAACCAGCATAACCAATGGCGTACAGGAATCCGTTCGGGAACAACAGcc                                                                                               >  1:1234891/1‑100 (MQ=255)
   tGCACGGGCTAACAGCGCCGGAACAATTGCCGCCCAGATGGTAGCCGCTAAACCAGCATAACCAATGGCGTACAGGAATCCGTTCGGGAACAACAGcccc                                                                                             >  1:3115478/1‑100 (MQ=255)
   tGCACGGGCTAACAGCGCCGGAACAATTGCCGCCCAGATGGTAGCCGCTAAACCAGCATAACCAATGGCGTACAGGAATCCGTTCGGGAACAACAGcccc                                                                                             >  2:2047260/1‑100 (MQ=255)
   tGCACGGGCTAACAGCGCCGGAACAATTGCCGCCCAGATGGTAGCCGCTAAACCAGCATAACCAATGGCGTACAGGAATCCGTTCGGGAACAACAGcccc                                                                                             >  1:139599/1‑100 (MQ=255)
    gCACGGGCTAACAGCGCCGGAACAATTTCCGCCCAGATGGTAGCCGCTAAACCAGCATAACCAATGGCGTACAGGAATCCGTTCGGGAACAACAGccccc                                                                                            >  2:2824050/1‑100 (MQ=255)
    gCACGGGCTAACAGCGCCGGAACAATTGCCGCCCAGATGGTAGCCGCTAAACCAGCATAACCAATGGCGTACAGGAATCCGTTCGGGAACAACAGccccc                                                                                            >  1:3607058/1‑100 (MQ=255)
     cACGGGCTAACAGCGCCGGAACAATTGCCGCCCAGATGGTAGCCGCTAAACCAGCATAACCAATGGCGTACAGGAATCCGTTCGGGAACAACAGcccccc                                                                                           <  2:4032784/100‑1 (MQ=255)
     cACGGGCTAACAGCGCCGGAACAATTGCCGCCCAGATGGTAGCCGCTAAACCAGCATAACCAATGGCGTACAGGAATCCGTTCGGGAACAACAGcccccc                                                                                           <  2:1197110/100‑1 (MQ=255)
     cACGGGCTAACAGCGCCGGAACAATTGCCGCCCAGATGGTAGCCGCTAAACCAGCATAAACAATGGCGTACAGGAATCCGTTCGGGAACAACAGccccac                                                                                           >  1:2042825/1‑98 (MQ=255)
     cACGGGCTAACAGCGCCGGAACAATCGCCGCCCAGATGGTAGCCGCTAAACCAGCATAACCAATGGCGTACAGGAATCCGTTCGGGAACAACAGcccccc                                                                                           <  1:2273632/100‑1 (MQ=255)
      aCGGGCTAACAGCGCCGGAACAATTGCCGCCCAGATGGTAGCCGCTAAACCAGCATAACCAATGGCGTACAGGAATCCGTTCGGGAACAACAGcccccca                                                                                          >  2:1066154/1‑99 (MQ=255)
       cGGGCTAACAGCGCCGGAACAATTGCCGCCCAGATGGTAGCCGCTAAACCAGCATAACCAATGGCGTACAGGAATCCGTTCGGGAACAACAGccccccac                                                                                         <  1:3032396/100‑3 (MQ=255)
       cGGGCTAACAGCGCCGGAACAATTGCCGCCCAGATGGTAGCCGCTAAACCAGCATAACCAATGGCGTACAGGAATCCGTTCGGGAACAACAGccccccac                                                                                         >  2:3769962/1‑98 (MQ=255)
            tAACAGCGCCGGAACAATTGCCGCCCAGATGGTAGCCGCTAAACCAGCATAACCAATGGCGTACAGGAATCCGTTCGGGAACAACAG‑CCCCCCACAACTg                                                                                   >  2:163780/1‑100 (MQ=255)
             aaCAGCGCCGGAACAATTGCCGCCCAGATGGTAGCCGCTAAACCAGCATAACCAATGGCGTACAGGAATCCGTTCGGGAACAACAG‑CCCCCCACAACTgg                                                                                  >  1:4032759/1‑100 (MQ=255)
               cAGCGCCGGAACAATTGCCGCCCAGATGGTAGCCGCTAAACCAGCATAACCAATGGCGTACAGGAATCCGTTCGGGAACAACAG‑CCCCCCACAACTGGCg                                                                                >  1:3841165/1‑100 (MQ=255)
               cAGCGCCGGAACAATTGCCGCCCAGATGGTAGCCGCTAAACCAGCATAACCAATGGCGTACAGGAATCCGTTCGGGAACAACAG‑CCCCCCACAACTGGCg                                                                                <  1:2311355/100‑1 (MQ=255)
               cAGCGCCGGAACAATTGCCGCCCAGATGGTAGCCGCTAAACCAGCATAACCAATGGCGTACAGGAATCCGGTCGGGAACAACAG‑CCCCCCACAACTGGCg                                                                                >  1:4145225/1‑100 (MQ=255)
               cAGCGCCGGAACAATTGCCGCCCAGATGGTAGCCGCTAAACCAGCATAACCAAAGGCGTACAGGAATCCGTTCGGGAACAACAG‑CCCCCCACAACTGCCg                                                                                >  1:397083/1‑100 (MQ=255)
                   gcCGGAACAATTGCCGCCCAGATGGTAGCCGCTAAACCAGCATAACCAATGGCGTACAGGAATCCGTTCGGGAACAACAG‑CCCCCCACAACTGGCGGGGc                                                                            <  1:925752/100‑1 (MQ=255)
                    ccGGAACAATTGCCGCCCAGATGGTAGCCGCTAAACCAGCATAACCAATGGCGTACAGGAATCCGTTCGGGAACAACAG‑CCCCCCACAACTGGCGGGGCa                                                                           >  2:3926264/1‑100 (MQ=255)
                     cGGAACAATTGCCGCCCAGATGGTAGCCGCTAAACCAGCATAACCAATGGCGTACAGGAATCCGTTCGGGAACAACAG‑CCCCCCACAACTGGCGGGGCaa                                                                          >  1:2127065/1‑100 (MQ=255)
                     cGGAACAATTGCCGCCCAGATGGTAGCCGCTAAACCAGCATAACCAATGGCGTACAGGAATCCGTTCGGGAACAACAG‑CCCCCCACAACTGGCGGGGCaa                                                                          >  1:3785204/1‑100 (MQ=255)
                     cGGAACAATTGCCGCCCAGATGGTAGCCGCTAAACCAGCATAACCAATGGCGTACAGGAATCCGTTCGGGAACAACAG‑CCCCCCACAACTGGCGGGGCaa                                                                          <  1:49829/100‑1 (MQ=255)
                     cGGAACAATTGCCGCCCAGATGGTAGCCGCTAAACCAGCATAACCAATGGCGTACAGGAATCCGTTCGGGAACAACAG‑CCCCCCACAACTGGCGGGGCaa                                                                          >  2:2399825/1‑100 (MQ=255)
                      ngAACAATTGCCGCCCAGATGGTAGCCGCTAAACCAGCATAACCAATGGCGTACAGGAATCCGTTCGGGAACAACAG‑CCCCCCACAACTGGCGGGGCaaa                                                                         >  1:2489058/2‑100 (MQ=255)
                      ggAACAATTGCCGCCCAGATGGTAGCCGCTAAACCAGCATAACCAATGGCGTACAGGAATCCGTTCGGGAACAACAG‑CCCCCCACAACTGGCGGGGCaaa                                                                         >  1:3242907/1‑100 (MQ=255)
                       gAACAATTGCCGCCCAGATGGTAGCCGCTAAACCAGCATAACCAATGGCGTACAGGAATCCGTTCGGGAACAACAG‑CCCCCCACAACTGGCGGGGCAAAg                                                                        <  2:3841122/100‑1 (MQ=255)
                       gAACAATTGCCGCCCAGATGGTAGCCGCTAAACCAGCATAACCAATGGCGTACAGGAATCCGTTCGGGAACAACAG‑CCCCCCACAACTGGCGGGGCAAAg                                                                        <  2:1042472/100‑1 (MQ=255)
                        aaCAATTGCCGCCCAGATGGTAGCCGCTAAACCAGCATAACCAATGGCGTACAGGAATCCGTTCGGGAACAACAG‑CCCCCCACAACTGGCGGGGCAAAgg                                                                       <  1:2146473/100‑1 (MQ=255)
                        aaCAATTGCCGCCCAGATGGTAGCCGCTAAACCAGCATAACCAATGGCGTACAGGAATCCGTTCGGGAACAACAG‑CCCCCCACAACTGGCGGGGCAAAgg                                                                       <  1:127178/100‑1 (MQ=255)
                         aCAATTGCCGCCCAGATGGTAGCCGCTAAACCAGCATAACCAATGGCGTACAGGAATCCGTTCGGGAACAACAG‑CCCCCCACAACTGGCGGGGCAAAGGt                                                                      >  1:1539533/1‑100 (MQ=255)
                         aCAATTGCCGCCCAGATGGTAGCCGCTAAACCAGCATAACCAATGGCGTACAGGAATCCGTTCGGGAACAACAG‑CCCCCCACAACTGGCGGGGCAAAGGt                                                                      <  1:1378450/100‑1 (MQ=255)
                          cAATTGCCGCCCAGATGGTAGCCGCTAACCCAGCATAACCAATGGCGTACAGGAATCCGTTCGGGAACAGCAG‑CCCCCCACAACTGGCGGGGCAAAGGTc                                                                     <  2:2620673/100‑1 (MQ=255)
                          cAATTGCCGCCCAGATGGTAGCCGCTAAACCAGCATAACCAATGGCGTACAGGAATCCGTTCGGGAACAACAG‑CCCCCCACAACTGGCGGGGCAAAGGTc                                                                     >  1:2678496/1‑100 (MQ=255)
                          cAATTGCCGCCCAGATGGTAGCCGCTAAACCAGCATAACCAATGGCGTACAGGAATCCGTTCGGGAACAACAG‑CCCCCCACAACTGGCGGGGCAAAGGTc                                                                     >  1:1794172/1‑100 (MQ=255)
                           aaTTGCCGCCCAGATGGTAGCCGCTAAACCAGCATAACCAATGGCGTACAGGAATCCGTTCGGGAACAACAG‑CCCCCCACAACTGGCGGGGCAAAGGTCa                                                                    <  2:2845251/100‑1 (MQ=255)
                           aaTTGCCGCCCAGATGGTAGCCGCTAAACCAGCATAACCAATGGCGTACAGGAATCCGTTCGGGAACAACAG‑CCCCCCACAACTGGCGGGGCAAAGGTCa                                                                    <  1:1426213/100‑1 (MQ=255)
                             ttGCCGCCCAGATGGTAGCCGCTAAACCAGCATAACCAATGGCGTACAGGAATCCGTTCGGGAACAACAG‑CCCCCCACAACTGGCGGGGCAAAGGTCAGc                                                                  <  1:850970/100‑1 (MQ=255)
                             ttGCCGCCCAGATGGTAGCCGCTAAACCAGCATAACCAATGGCGTACAGGAATCCGTTCGGGAACAACAG‑CCCCCCACAACTGGCGGGGCAAAGGTCAGc                                                                  <  2:1780604/100‑1 (MQ=255)
                             ttGCCGCCCAGATGGTAGCCGCTAAACCAGCATAACCAATGGCGTACAGGAATCCGTTCGGGAACAACAG‑CCCACCACAACTGGCGGGGCAAAGGTCAGc                                                                  <  2:1236144/100‑1 (MQ=255)
                              tGCCGCCCAGATGGTAGCCGCTAAACCAGCATAACCAATGGCGTACAGGAATCCGTTCGGGAACAACAG‑CCCCCCACAACTGGCGGGGCAAAGGTCAGCa                                                                 <  1:2008009/100‑1 (MQ=255)
                                ccgccCAGATGGTAGCCGCTAAACCAGCATAACCAATGGCGTACAGGAATCCGTTCGGGAACATCAG‑CCCCCCACAACTGGCGGGGCAAAGGTCAGCAAt                                                               <  1:2952875/100‑1 (MQ=255)
                                 cgccCAGATGGTAGCCGCTAAACCAGCATAACCAATGGCGTACAGGAATCCGTTCGGGAACAACAG‑CCCCCCACAACTGGCGGGGCAAAGGTCAGCAATg                                                              <  2:2727160/100‑1 (MQ=255)
                                  gccCAGATGGTAGCCGCTAAACCAGCATAACCAATGGCGTACAGGAATCCGTTCGGGAACAACAG‑CCCCCCACAACTGGCGGGGCAAAGGTCAGCAATGc                                                             >  2:277679/1‑100 (MQ=255)
                                    ccAGATGGTAGCCGCTAAACCAGCATAACCAATGGCGTACAGGAATCCGTTCGGGAACAACAG‑CCCCCCACAACTGGCGGGGCAAAGGTCAGCAATGCCg                                                           >  1:3904074/1‑100 (MQ=255)
                                     cAGATGGTAGCCGCTAAACCAGCATAACCAATGGCGTACAGGAATCCGTTCGGGAACAACAG‑CCCCCCACAACTGGCGGGGCAAAGGTCAGCAATGCCGt                                                          >  2:1401621/1‑100 (MQ=255)
                                       gATGGTAGCCGCTAAACCAGCATAACCAATGGCGTACAGGAATCCGTTCGGGAACAACAG‑CCCCCCACAACTGGCGGGGCAAAGGTCAGCAATGCCGttt                                                        <  1:2536353/100‑1 (MQ=255)
                                         tggtggCCGCTAAACCAGCATCACCAATGGCGTACAGGACTGCGTTCGGGAACAACAG‑CCCCCCACAACTGGCGGGGCAAAGGTCAGCAATGCCGTTTTc                                                      <  2:2493998/100‑1 (MQ=255)
                                         tGGTAGCCGCTAAACCAGCATAACCAATGGCGTACAGGAATCCGTTCGGGAACAACAG‑CCCCCCACAACTGGCGGGGCAAAGGTCAGCAATGCCGTTTTc                                                      <  1:1707524/100‑1 (MQ=255)
                                            tAGCCGCTAAACCAGCATAACCATTGGTGTACAGTAATCCGTTCGGGAATAACAG‑CCCCCCACAACTGGCGGGGCAAAGTTCAGTAATGCCGTTTTCAAg                                                   <  2:2486083/100‑1 (MQ=255)
                                            tAGCCGCTAAACCAGCATAACCAATGGCGTACAGGAATCCGTTCGGGAACAACAG‑CCCCCCACAACTGGCGGGGCAAAGGTCAGCAATGCCGTTTTCAAg                                                   <  1:791806/100‑1 (MQ=255)
                                             aGCCGCCAAACCAGCATAACCAATCGCGTACAGGAATCCGTTCGGGAACAACAG‑CCCCCCACAACTGGCGGGGCAAAGGTCAGCAATGCCGTTTTCAAg                                                   <  2:2042810/99‑1 (MQ=255)
                                               ccGCTAAACCAGCATAACCAATGGCGTACAGGAATCCGTTCGGGAACAACAG‑CCCCCCACAACTGGCGGGGCAAAGGTCAGCAATGCCGTTTTCAAGCgg                                                >  2:1979126/1‑100 (MQ=255)
                                                cGCTAAACCAGCATAACCAATGGCGTACAGGAATCCGTTCGGGAACAACAG‑CCCCCCACAACTGGCGGGGCAAAGGTCAGCAATGCCGTTTTCAAGCGGc                                               <  2:2974177/100‑1 (MQ=255)
                                                      aCCAGCATAACCAATGGCGTACAGGAATCCGTTCGGGAACAACAG‑CCCCCCACAACTGGCGGGGCAAAGGTCAGCAATGCCGTTTTCAAGCGGCCCACAg                                         >  1:127687/1‑100 (MQ=255)
                                                      aCCAGCATAACCAATGGCGTACAGGAATCCGTTCGGGAACAACAG‑CCCCCCACAACTGGCGGGGCAAAGGTCAGCAATGCCGTTTTCAAGCGGCCCACAg                                         >  2:2037752/1‑100 (MQ=255)
                                                       ccAGCATAACCAATGGCGTACAGGAATCCGTTCGGGAACAACAG‑CCCCCCACAACTGGCGGGGCAAAGGTCAGCAATGCCGTTTTCAAGCGGCCCACAGc                                        <  2:181323/100‑1 (MQ=255)
                                                       ccAGCATAACCAATGGCGTACAGGAATCCGTTCGGGAACAACAG‑CCCCCCACAACTGGCGGGGCAAAGGTCAGCAATGCCGTTTTCAAGCGGCCCACAGc                                        <  1:3518657/100‑1 (MQ=255)
                                                        cAGCATAACCAATGGCGTACAGGAATCCGTTCGGGAACAACAG‑CCCCCCACAACTGGCGGGGCAAAGGTCAGCAATGCCGTTTTCAAGCGGCCCACAGcc                                       >  1:3141061/1‑100 (MQ=255)
                                                         aGCATAACCAATGGCGTACAGGAATCCGTTCGGGAACAACAG‑CCCCCCACAACTGGCGGGGCAAAGGTCAGCAATGCCGTTTTCAAGCGGCCCACAGCCg                                      <  2:3134642/100‑1 (MQ=255)
                                                          gCATAACCAATGGCGTACAGGAATCCGTTCGGGAACAACAG‑CCCCCCACAACTGGCGGGGCAAAGGTCAGCAATGCCGTTTTCAAGCGGCCCACAGCCg                                      <  1:3770793/99‑1 (MQ=255)
                                                          gCATAACCAATGGCGTACAGGAATCCGTTCGGGAACAACAG‑CCCCCCACAACTGGCGGGGCAAAGGTCAGCAATGCCGTTTTCAAGCGGCCCACAGCCGa                                     >  2:1007794/1‑100 (MQ=255)
                                                               aCCCATGGCGTACAGGAATCCGTTCGGGAACAACAG‑CCCCCCACAACTGGCGGGGCAAAGGTCAGCAATGCCGTTTTCAAGCGGCCCACAGCCGAgtcn                                 <  2:2489033/99‑2 (MQ=255)
                                                                ccAATGGCGTACAGGAATCCGTTCGGGAACAACAG‑CCCCCCACAACTGGCGGGGCAAAGGTCAGCAATGCCGGTTTCAAGCGGCCCACAGCCGAgtcgtc                               >  1:1776577/1‑100 (MQ=255)
                                                                 cAATGGCGTACAGGAATCCGTTCGGGAACAACAG‑CCCCCCACAACTGGCGGGGCAAAGGTCAGCAATGCCGTTTTCAAGCGGCCCACAGCCGAGtcgtcg                              <  2:4161258/100‑1 (MQ=255)
                                                                 cAATGGCGTACAGGAATCCGTTCGGGAACAACAG‑CCCCCCACAACTGGCGGGGCAAAGGTCAGCAATGCCGTTTTCAAGCGGCCCACAGCCGAGtcgtcg                              >  1:3572105/1‑100 (MQ=255)
                                                                 cAATGGCGTACAGGAATCCGTTCGGGAACAACAG‑CCCCCCACAACTGGCGGGGCAAAGGTCAGCAATGCCGTTTTCAAGCGGCCCACAGCCGAGtcgtcg                              <  1:458675/100‑1 (MQ=255)
                                                                     ggCGTACAGGAATCCGTTCGGGAACAACAG‑CCCCCCACAACTGGCGGGGCATAGGTCAGCAATGCCGTTTTCAAGCGGCCCACAGCCGAGTCGTCGAAAc                          >  1:4162954/1‑100 (MQ=255)
                                                                      gCGTACAGGAATCCGTTCGGGAACAACAG‑CCCCCCACAACTGGCGGGGCAAAGGTCAGCAATGCCGTTTTCAAGCGGCCCACAGCCGAGTCGTCGAAAcc                         <  2:296124/100‑1 (MQ=255)
                                                                          aCAGGAATCCGTTCGGGAACAACAG‑CCCCCCACAACTGGCGGGGCAAAGGTCAGCAATGCCGTTTTCAAGCGGCCCACAGCCGAGTCGTCGAAACCAAAc                     <  2:321959/100‑1 (MQ=255)
                                                                           cAGGAATCCGTTCGGGAACAACAG‑CCCCCCACAACTGGCGGGGCAAAGGTCAGCAATGCCGTTTTCAAGCGGCCCACAGCCGAGTCGTCGAAACCAAACa                    >  1:1418747/1‑100 (MQ=255)
                                                                            aGGAATCCGTTCGGGAACAACAG‑CCCCCCACAACTGGCGGGGCAAAGGTCAGCAATGCCGTTTTCAAGCGGCCCACAGCCGAGTCGTCGAAACCAAACAg                   <  1:115874/100‑1 (MQ=255)
                                                                             ggAATCCGTTCGGGAACAACAG‑CCCCCCACAACTGGCGGGGCAAAGGTCAGCAATGCCGTTTTCAAGCGGCCCACCGCCGAGTCGTCGCAACCAAACCGa                  >  1:890747/1‑100 (MQ=255)
                                                                             ggAATCCGTTCGGGAACAACAG‑CCCCCCACAACTGGCGGGGCAAAGGTCAGCAATGCCGTTTTCAAGCGGCCCACAGCCGAGTCGTCGAAACCAAACAGa                  >  1:2360970/1‑100 (MQ=255)
                                                                             ggAATCCGTTCGGGAACAACAG‑CCCCCCACAACTGGCGGGGCAAAGGTCAGCAATGCCGTTTTCAAGCGGCCCACAGCCGAGTCGTCGAAACCAAACAGa                  >  1:3151011/1‑100 (MQ=255)
                                                                              gAATCCGTTCGGGAACAACAG‑CCCCCCACAACTGGCGGGGCAAAGGTCAGCAATGCCGTTTTCAAGCGGCCCACAGCCGAGTCGTCGAAACCAAACAGAt                 <  2:4032716/100‑1 (MQ=255)
                                                                                 tCCGTTCGGGAACAACAG‑CCCCCCACAACTGGCGGGGCAAAGGTCAGCAATGCCGTTTTCAAGCGGCCCACAGCCGAGTCGTCGAAACCAAACAGATCTg              <  1:338801/100‑1 (MQ=255)
                                                                                   cGTTCGGGAACAACAGCCCCCCCACAACTGGCGGGGCAAAGGTCAGCAATGCCGTTTTCAAGCGGCCCACAGCCGAGTCGTCGAAACCAAACAGATCTGc             >  1:2367099/1‑100 (MQ=255)
                                                                                   cGTTCGGGAACAACAG‑CCCCCCACAACTGGCGGGGCAAAGGTCAGCAATGCCGTTTTCAAGCGGCCCACAGCCGAGTCGTCGAAACCAAACAGATCTGcc            <  1:2716337/100‑1 (MQ=255)
                                                                                    gTTCGGGAACAACAG‑CCCCCCACAACTGGCGGGGCAAAGGTCAGCAATGCCGTTTTCAAGCGGCCCACAGCCGAGTCGTCGAAACCAAACAGATCTGCCa           >  1:1959120/1‑100 (MQ=255)
                                                                                     ttCGGGAACAACAG‑CCCCCCACAACTGGCGGGGCAAAGGTCAGCAATGCCGTTTTCAAGCGGCCCACAGCCGAGTCGTCGAAACCAAACAGATCTGCCAg          >  1:1976170/1‑100 (MQ=255)
                                                                                     ttCGGGAACAACAG‑CCCCCCACAACTGGCGGGGCAAAGGTCAGCAATGCCGTTTTCAAGCGGCCCACAGCCGAGTCGTCGAAACCAAACAGATCTGCCAg          >  1:2907884/1‑100 (MQ=255)
                                                                                       cGGGAACAACAG‑CCCCCCACAACTGGCGGGGCAAAGGTCAGCAATGCCGTTTTCAAGCGGCCCACAGCCGAGTCGTCGAAACCAAACAGATCTGCCAGAt        >  1:3429299/1‑100 (MQ=255)
                                                                                        gggAACAACAG‑CCCCCCACAACTGGCGGGGCAAAGGTCAGCAATGCCGTTTTCAAGCGGCCCACAGCCGAGTCGTCGAAACCAAACAGATCTGCCAGATa       >  2:227620/1‑100 (MQ=255)
                                                                                        gggAACAACAG‑CCCCCCACAACTGGCGGGGCAAAGGTCAGCAATGCCGTTTTCAAGCGGCCCACAGCCGAGTCGTCGAAACCAAACAGATCTGCCAGATa       >  1:621079/1‑100 (MQ=255)
                                                                                         ggAACAACAG‑CCCCCCACAACTGGCGGGGCAAAGGTCAGCAATGCCGTTTTCAAGCGGCCCACAGCCGAGTCGTCGAAACCAAACAGATCTGCCAGATAg      <  1:1672238/100‑1 (MQ=255)
                                                                                          gAACAACAG‑CCCCCCACAACTGGCGGGGCAAAGGTCAGCAATGCCGTTTTCAAGCGGCCCACAGCCGAGTCGTCGAAACCAAACAGATCTGCCAGATAGt     >  1:2559993/1‑100 (MQ=255)
                                                                                           aacaacAG‑CCCCCCACAACTGGCGGGGCAAAGGTCAGCAATGCCGTTTTCAAGCGGCCCACAGCCGAGTCGTCGAAACCAAACAGATCTGCCAGATAGTc    <  2:1436729/100‑1 (MQ=255)
                                                                                            acaacaG‑CCCCCCACAACTGGCGGGGCAAAGGTCAGCAATGCCGTTTTCAAGCGGCCCACAGCCGAGTCGTCGAAACCAAACAGATCTGCCAGATAGTca   <  2:3133108/100‑1 (MQ=255)
                                                                                            acaacaG‑CCCCCCACAACTGGCGGGGCAAAGGTCAGCAATGCCGTTTTCAAGCGGCCCACAGCCGAGTCGTCGAAACCAAACAGATCTGCCAGATAGTca   >  1:560958/1‑100 (MQ=255)
                                                                                            acaacaG‑CCCCCCACAACTGGCGGGGCAAAGGTCAGCAATGCCGTTTTCAAGCGGCCCACAGCCGAGTCGTCGAAACCAAACAGATCTGCCAGATAGTca   <  1:1664076/100‑1 (MQ=255)
                                                                                            acaacaG‑CCCCCCACAACTGGCGGGGCAAAGGTCAGCAATGCCGTTTTCAAGCGGCCCACACCCGAGTCGTCGAAACCAAACAGATTTGCCAGATAGTca   <  2:2583562/100‑1 (MQ=255)
                                                                                             caacaG‑CCCCCCACAACTGGCGGGGCAAAGGTCAGCAATGCCGTTTTCAAGCGGCCCACAGCCGAGTCGTCGAAACCAAACAGATCTGCCAGATAGTcaa  <  1:355158/100‑1 (MQ=255)
                                                                                             caacaG‑CCCCCCACAACTGGCGGGGCAAAGGTCAGCAATGCCGTTTTCAAGCGGCCCACAGCCGAGTCGTCGAAACCAAACAGATCTGCCAGATAGTcaa  >  2:2333583/1‑100 (MQ=255)
                                                                                             caacaG‑CCCCCCACAACTGGCGGGGCAAAGGTCAGCAATGCCGTTTTCAAGCGGCCCACAGCCGAGTCGTCGAAACCAAACAGATCTGCCAGATAGTcaa  >  2:3380558/1‑100 (MQ=255)
                                                                                             caacaG‑CCCCCCACAACTGGCGGGGCAAAGGTCAGCAATGCCGTTTTCAAGCGGCCCACAGCCGAGTCGTCGAAACCAAACAGATCTGCCAGATAGTcaa  >  2:11313/1‑100 (MQ=255)
                                                                                             caacaG‑CCCCCCACAACTGGCGGGGCAAAGGTCAGCAATGCCGTTTTCAAGCGGCCCACAGCCGAGTCGTCGAAACCAAACAGATCTGCCAAACAGTca   >  2:3499242/1‑99 (MQ=255)
                                                                                             caacaG‑CCCCCCACAACTGGCGGGGCAAAGGTCAGCAATGCCGTCTTCAAGCGGCCCACAGCCGAGTCGTCGCAACCAAACAGATCTGCCAGACACTcaa  >  2:1778430/1‑100 (MQ=255)
                                                                                                   |                                                                                              
CGATGCACGGGCTAACAGCGCCGGAACAATTGCCGCCCAGATGGTAGCCGCTAAACCAGCATAACCAATGGCGTACAGGAATCCGTTCGGGAACAACAGCCCCCCCACAACTGGCGGGGCAAAGGTCAGCAATGCCGTTTTCAAGCGGCCCACAGCCGAGTCGTCGAAACCAAACAGATCTGCCAGATAGTCAA  >  NC_000913/3304708‑3304901

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A