Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A4 F191 I1 R1 67 105.9 5535243 92.7% 5131170 99.9

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 3,951,559 +A pseudogene (18/663 nt) ilvG → pseudogene, acetolactate synthase 2 large subunit, valine‑insensitive; acetolactate synthase II, large subunit, cryptic, interrupted

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,951,5591.A100.0% 305.1 / NA 85pseudogene (18/663 nt)ilvGpseudogene, acetolactate synthase 2 large subunit, valine‑insensitive; acetolactate synthase II, large subunit, cryptic, interrupted
Reads supporting (aligned to +/- strand):  ref base . (0/0);  new base A (46/39);  total (46/39)

AACAAGCTGCGTCAGGCACATGTGGCATTACAAGGTGATTTAAATGCTCTGTTACCAGCATTACAGCAGCCGTTAAATCAATGACTGGCAGCAACACTGC‑GCGCAGCTGCGTGATGAACATTCCTGGCGTTACGACCATCCCGGTGACGCTATCTACGCGCCGTTGTTGTTAAAACAACTGTCGGATCGTAAA  >  NC_000913/3951460‑3951652
                                                                                                    |                                                                                             
aaCAAGCTGCGTCAGGCACATGTGGCATTACAAGGTGATTTAAATGCTCTGTTACCAGCATTACAGCAGCCGTTAAATCAATGACTGGCAGCAACACTgc                                                                                                <  1:1836179/100‑1 (MQ=255)
aaCAAGCTGCGTCAGGCACATGTGGCATTACAAGGTGATTTAAATGCTCTGTTACCAGCATTACAGCAGCCGTTAAATCAATGACTGGCAGCAACACTgc                                                                                                <  2:443452/100‑1 (MQ=255)
     gCTGCGTCAGGCACATGTGGCATTACAAGGTGATTTAAATGCTCTGTTACCAGCATTACAGCAGCCGTTAAATCAATGACTGGCAGCAACACTgcagcgc                                                                                           <  2:148922/100‑6 (MQ=255)
      cTGCGTCAGGCACATGTGGCATTACAAGGTGATTTAAATGCTCTGTTACCAGCATTACAGCAGCCGTTAAATCAATGACTGGCAGCAACACTGCAGCGCa                                                                                          <  1:758328/100‑1 (MQ=255)
         cGTCAGGCACATGTGGCATTACAAGGTGATTTAAATGCTCTGTTACCAGCATTACAGCAGCCGTTAAATCAATGACTGGCAGCAACACTGCAGCGCAGCt                                                                                       <  2:1492174/100‑1 (MQ=255)
            cAGGCACATGTGGCATTACAAGGTGATTTAAATGCTCTGTTACCAGCATTACAGCAGCCGTTAAATCAATGACTGGCAGCAACACTGCAGCGCAGCTGCg                                                                                    <  2:2038116/100‑1 (MQ=255)
             aGGCACATGTGGCATTACAAGGTGATTTAAATGCTCTGTTACCAGCATTACAGCAGCCGTTAAATCAATGACTGGCAGCAACACTGCAGCGCAGCTGCGt                                                                                   <  2:156978/100‑1 (MQ=255)
              ggCACATGTGGCATTACAAGGTGATTTAAATGCTCTGTTACCAGCATTACAGCAGCCGTTAAATCAATGACTGGCAGCAACACTGCAGCGCAGCTGCGtg                                                                                  >  1:628391/1‑100 (MQ=255)
              ggCACATGTGGCATTACAAGGTGATTTAAATGCTCTGTTACCAGCATTACAGCAGCCGTTAAATCAATGACTGGCAGCAACACTGCAGCGCAGCTGCGtg                                                                                  >  2:1626275/1‑100 (MQ=255)
              ggCACATGTGGCATTACAAGGTGATTTAAATGCTCTGTTACCAGCATTACAGCAGCCGTTAAATCAATGACTGGCAGCAACACTGCAGCGCAGCTGCGtg                                                                                  >  2:538873/1‑100 (MQ=255)
               gCACATGTGGCATTACAAGGTGATTTAAATGCTCTGTTACCAGCATTACAGCAGCCGTTAAATCAATGACTGGCAGCAACACTGCAGCGCAGCTGCGtga                                                                                 <  2:2427158/100‑1 (MQ=255)
                 acaTGTGGCATTACAAGGTGATTTAAATGCTCTGTTACCAGCATTACAGCAGCCGTTAAATCAATGACTGGCAGCAACACTGCAGCGCAGCTGCGtgatg                                                                               <  2:487138/100‑1 (MQ=255)
                    tgtgGCATTACAAGGTGATTTAAATGCTCTGTTACCAGCATTACAGCAGCCGTTAAATCAATGACTGGCAGCAACACTGCAGCGCAGCTGCGTGATGAAc                                                                            >  2:2113527/1‑100 (MQ=255)
                      tgGCATTACAAGGTGATTTAAATGCTCTGTTACCAGCATTACAGCAGCCGTTAAATCAATGACTGGCAGCAACACTGCAGCGCAGCTGCGTGATGAACAt                                                                          >  1:1756054/1‑100 (MQ=255)
                       ggCATTACAAGGTGATTTAAATGCTCTGTTACCAGCATTACAGCAGCCGTTAAATCAATGACTGGCAGCAACACTGCAGCGCAGCTGCGTGATGAACAtt                                                                         >  1:2229569/1‑100 (MQ=255)
                         cATTACAAGGTGATTTAAATGCTCTGTTACCAGCATTACAGCAGCCGTTAAATCAATGACTGGCAGCAACACTGCAGCGCAGCTGCGTGATGAACATTcc                                                                       <  1:2113544/100‑1 (MQ=255)
                         cATTACAAGGTGATTTAAATGCTCTGTTACCAGCATTACAGCAGCCGTTAAATCAATGACTGGCAGCAACACTGCAGCGCAGCTGCGTGATGAACATTcc                                                                       <  1:1280700/100‑1 (MQ=255)
                         cATTACAAGGTGATTTAAATGCTCTGTTACCAGCATTACAGCAGCCGTTAAATCAATGACTGGCAGCAACACTGCAGCGCAGCTGCGTGATGAACATTcc                                                                       <  1:1504558/100‑1 (MQ=255)
                          aTTACAAGGTGATTTAAATGCTCTGTTACCAGCATTACAGCAGCCGTTAAATCAATGACTGGCAGCAACACTGCAGCGCAGCTGCGTGATGAACATTCCt                                                                      >  1:209529/1‑100 (MQ=255)
                           ttACAAGGTGATTTAAATGCTCTGTTACCAGCATTACAGCAGCCGTTAAATCAATGACTGGCAGCAACACTGCAGCGCAGCTGCGTGATGAACATTCCTg                                                                     <  2:60026/100‑1 (MQ=255)
                           ttACAAGGTGATTTAAATGCTCTGTTACCAGCATTACAGCAGCCGTTAAATCAATGACTGGCAGCAACACTGCAGCGCAGCTGCGTGATGAACATTCCTg                                                                     <  2:718159/100‑1 (MQ=255)
                              cAAGGTGATTTAAATGCTCTGTTACCAGCATTACAGCAGCCGTTAAATCAATGACTGGCAGCAACACTGCAGCGCAGCTGCGTGATGAACATTCCTGGCg                                                                  <  1:2097528/100‑1 (MQ=255)
                              cAAGGTGATTTAAATGCTCTGTTACCAGCATTACAGCAGCCGTTAAATCAATGACTGGCAGCAACACTGCAGCGCAGCTGCGTGATGAACATTCCTGGCg                                                                  >  1:191791/1‑100 (MQ=255)
                              cAAGGTGATTTAAATGCTCTGTTACCAGCATTACAGCAGCCGTTAAATCAATGACTGGCAGCAACACTGCAGCGCAGCTGCGTGATGAACATTCCTGGCg                                                                  >  2:2235205/1‑100 (MQ=255)
                              cAAGGTGATTTAAATGCTCTGTTACCAGCATTACAGCAGCCGTTAAATCAATGACTGGCAGCAACACTGCAGCGCAGCTGCGTGATGAACATTCCTGGCg                                                                  >  2:2291945/1‑100 (MQ=255)
                               aaGGTGATTTAAATGCTCTGTTACCAGCATTACAGCAGCCGTTAAATCAATGACTGGCAGCAACACTGCAGCGCAGCTGCGTGATGAACATTCCTGGCGt                                                                 >  1:763055/1‑100 (MQ=255)
                                aGGTGATTTAAATGCTCTGTTACCAGCATTACAGCAGCCGTTAAATCAATGACTGGCAGCAACACTGCAGCGCAGCTGCGTGATGAACATTCCTGGCGtt                                                                >  1:683753/1‑100 (MQ=255)
                                aGGTGATTTAAATGCTCTGTTACCAGCATTACAGCAGCCGTTAAATCAATGACTGGCAGCAACACTGCAGCGCAGCTGCGTGATGAACATTCCTGGCGtt                                                                <  2:1007906/100‑1 (MQ=255)
                                aGGTGATTTAAATGCTCTGTTACCAGCATTACAGCAGCCGTTAAATCAATGACTGGCAGCAACACTGCAGCGCAGCTGCGTGATGAACATTCCTGGCGtt                                                                >  1:840141/1‑100 (MQ=255)
                                  gTGATTTAAATGCTCTGTTACCAGCATTACAGCAGCCGTTAAATCAATGACTGGCAGCAACACTGCAGCGCAGCTGCGTGATGAACATTCCTGGCGTTAc                                                              <  1:792894/100‑1 (MQ=255)
                                      tttAAATGCTCTGTTACCAGCATTACAGCAGCCGTTAAATCAATGACTGGCAGCAACACTGCAGCGCAGCTGCGTGATGAACATTCCTGGCGTTACGAcc                                                          >  1:1951891/1‑100 (MQ=255)
                                        tAAATGCTCTGTTACCAGCATTACAGCAGCCGTTAAATCAATGACTGGCAGCAACACTGCAGCGCAGCTGCGTGATGAACATTCCTGGCGTTACGACCAt                                                        >  2:958304/1‑100 (MQ=255)
                                        tAAATGCTCTGTTACCAGCATTACAGCAGCCGTTAAATCAATGACTGGCAGCAACACTGCAGCGCAGCTGCGTGATGAACATTCCTGGCGTTACGACCAt                                                        >  1:1545686/1‑100 (MQ=255)
                                            tGCTCTGTTACCAGCATTACAGCAGCCGTTAAATCAATGACTGGCAGCAACACTGCAGCGCAGCTGCGTGATGAACATTCCTGGCGTTACGACCATCCCg                                                    >  1:2367742/1‑100 (MQ=255)
                                             gCTCTGTTACCAGCATTACAGCAGCCGTTAAATCAATGACTGGCAGCAACACTGCAGCGCAGCTGCGTGATGAACATTCCTGGCGTTACGACCATCCCgg                                                   <  1:2379345/100‑1 (MQ=255)
                                             gCTCTGTTACCAGCATTACAGCAGCCGTTAAATAAATGACTGGCAGCAACACTGCAGCGCAGCTGCGTGATGAACATTCCTGGCGTTACGACCATCCCgg                                                   <  2:1053356/100‑1 (MQ=255)
                                              ctctGTTACCAGCATTACAGCAGCCGTTAAATCAATGACTGGCAGCAACACTGCAGCGCAGCTGCGTGATGCACCTTCCTGGCGTTACGACCATCCCGGt                                                  >  2:2419405/1‑100 (MQ=255)
                                              ctctGTTACCAGCATTACAGCAGCCGTTAAATCAATGACTGGCAGCAACACTGCAGCGCAGCTGCGTGATGAACATTCCTGGCGTTACGACCATCCCGGt                                                  >  2:847818/1‑100 (MQ=255)
                                               tctGTTACCAGCATTACAGCAGCCGTTAAATCAATGACTGGCAGCAACACTGCAGCGCAGCTGCGTGATGAACATTCCTGGCGTTACGACCATCCCGGTg                                                 <  2:683748/100‑1 (MQ=255)
                                                ctGTTACCAGCATTACAGCAGCCGTTAAATCAATGACTGGCAGCAACACTGCAGCGCAGCTGCGTGATGAACATTCCTGGCGTTACGACCATCCCGGTGa                                                <  1:1170558/100‑1 (MQ=255)
                                                 tGTTACCAGCATTACAGCAGCCGTTAAATCAATGACTGGCAGCAACACTGCAGCGCAGCTGCGTGATGAACATTCCTGGCGTTACGACCATCCCGGTGAc                                               <  2:369691/100‑1 (MQ=255)
                                                 tGTTACCAGCATTACAGCAGCCGTTAAATCAATGACTGGCAGCAACACTGCAGCGCAGCTGCGTGATGAACATTCCTGGCGTTACGACCATCCCGGTGAc                                               >  2:967226/1‑100 (MQ=255)
                                                  gTTACCAGCATTACAGCAGCCGTTAAATCAATGACTGGCAGCAACACTGCAGCGCAGCTGCGTGATGAACATTCCTGGCGTTACGACCATCCCGGTGACg                                              <  2:238679/100‑1 (MQ=255)
                                                  gTTACCAGCATTACAGCAGCCGTTAAATCAATGACTGGCAGCAACACTGCAGCGCAGCTGCGTGATGAACATTCCTGGCGTTACGACCATCCCGGTGACg                                              >  2:2011771/1‑100 (MQ=255)
                                                     aCCAGCATTACAGCAGCCGTTAAATCAATGACTGGCAGCAACACTGCAGCGCAGCTGCGTGATGAACATTCCTGGCGTTACGACCATCCCGGTGACGCTa                                           >  1:2660185/1‑100 (MQ=255)
                                                      ccAGCATTACAGCAGCCGTTAAATCAATGACTGGCAGCAACACTGCAGCGCAGCTGCGTGATGAACATTCCTGGCGTTACGACCATCCCGGTGACGCTAt                                          >  2:44428/1‑100 (MQ=255)
                                                       cAGCATTACAGCAGCCGTTAAATCAATGACTTGCAGCAACACCGCAGCGCAGCTGCGTGATGAACATTCCTGGCGTTACGACCATCCCGGTGACGCTATc                                         <  1:592987/100‑1 (MQ=255)
                                                       cAGCATTACAGCAGCCGTTAAATCAATGACTGGCAGCAACACTGCAGCGCAGCTGCGTGATGAACATTCCTGGCGTTACGACCATCCCGGTGACGCTATc                                         >  2:1782538/1‑100 (MQ=255)
                                                          cATTACAGCAGCCGTTAAATCAATGACTGGCAGCAACACTGCAGCGCAGCTGCGTGATGAACATTCCTGGCGTTACGACCATCCCGGTGACGCTATCTAc                                      >  2:2592979/1‑100 (MQ=255)
                                                            ttACAGCAGCCGTTAAATCAATGACTGGCAGCAACACTGCAGCGCAGCTGCGTGATGAACATTCCTGGCGTTACGACCATCCCGGTGACGCTATCTAcgc                                    >  1:101138/1‑100 (MQ=255)
                                                            ttACAGCAGCCGTTAAATCAATGACTGGCAGCAACACTGCAGCGCAGCTGCGTGATGAACATTCCTGGCGTTACGACCATCCCGGTGACGCTATCTAcgc                                    >  1:1018360/1‑100 (MQ=255)
                                                                agcagcCGTTAAATCAATGACTGGCAGCAACACTGCAGCGCAGCTGCGTGATGAACATTCCTGGCGTTACGACCATCCCGGTGACGCTATCTACGCGCCg                                >  2:1033325/1‑100 (MQ=255)
                                                                 gcagcCGTTAAATCAATGACTGGCAGCAACACTGCAGCGCAGCTGCGTGATGAACATTCCTGGCGTTACGACCATCCCGGTGACGCTATCTACGCGCCgt                               >  1:2239882/1‑100 (MQ=255)
                                                                 gcagcCGTTAAATCAATGACTGGCAGCAACACTGCAGCGCAGCTGCGTGATGAACATTCCTGGCGTTACGACCATCCCGGTGACGCTATCTACGCGCCgt                               >  2:2109781/1‑100 (MQ=255)
                                                                  cagcCGTTAAATCAATGACTGGCAGCAACACTGCAGCGCAGCTGCGTGATGAACATTCCTGGCGTTACGACCATCCCGGTGACGCTATCTACGCGCCgtt                              >  1:449492/1‑100 (MQ=255)
                                                                    gcCGTTAAATCAATGACTGGCAGCAACACTGCAGCGCAGCTGCGTGATGAACATTCCTGGCGTTACGACCATCCCGGTGACGCTATCTACGCGCCgttgt                            >  2:1748685/1‑100 (MQ=255)
                                                                    gcCGTTAAATCAATGACTGGCAGCAACACTGCAGCGCAGCTGCGTGATGAACATTCCTGGCGTTACGACCATCCCGGTGACGCTATCTACGCGCCgttgt                            >  2:120601/1‑100 (MQ=255)
                                                                     ccGTTAAATCAATGACTGGCAGCAACACTGCAGCGCAGCTGCGTGATGAACATTCCTGGCGTTACGACCATCCCGGTGACGCTATCTACGCGCCgttgt                            <  2:680116/99‑1 (MQ=255)
                                                                      cGTTAAATCAATGACTGGCAGCAACACTGCAGCGCAGCTGCGTGATGAACATTCCTGGCGTTACGACCATCCCGGTGACGCTATCTACGCGCCgttgttg                          <  2:2560717/100‑1 (MQ=255)
                                                                         tAAATCAATGACTGGCAGCAACACTGCAGCGCAGCTGCGTGATGAACATTCCTGGCGTTACGACCATCCCGGTGACGCTATCTACGCGCCGTTGTTGTTa                       >  2:1182281/1‑100 (MQ=255)
                                                                           aaTCAATGACTGGCAGCAACACTGCAGCGCAGCTGCGTGATGAACATTCCTGGCGTTACGACCATCCCGGTGACGCTATCTACGCGCCGTTGTTGTTaaa                     >  2:2323588/1‑100 (MQ=255)
                                                                           aaTCAATGACTGGCAGCAACACTGCAGCGCAGCTGCGTGATGAACATTCCTGGCGTTACGACCATCCCGGTGACGCTATCTACGCGCCGTTGTTGTTaaa                     <  2:780990/100‑1 (MQ=255)
                                                                           aaTCAATGACTGGCAGCAACACTGCAGCGCAGCTGCGTGATGAACATTCCTGGCGTTACGACCATCCCGGTGACGCTATCTACGCGCCGTTGTTGTTaaa                     <  2:415543/100‑1 (MQ=255)
                                                                            aTCAATGACTGGCAGCAACACTGCAGCGCAGCTGCGTGATGAACATTCCTGGCGTTACGACCATCCCGGTGACGCTATCTACGCGCCGTTGTTGTTaaaa                    >  1:127029/1‑100 (MQ=255)
                                                                             tCAATGACTGGCAGCAACACTGCAGCGCAGCTGCGTGATGAACATTCCTGGCGTTACGACCATCCCGGTGACGCTATCTACGCGCCGTTGTTGTTAAaac                   <  2:2261180/100‑1 (MQ=255)
                                                                             tCAATGACTGGCAGCAACACTGCAGCGCAGCTGCGTGATGAACATTCCTGGCGTTACGACCATCCCGGTGACGCTATCTACGCGCCGTTGTTGTTAAaac                   >  2:959531/1‑100 (MQ=255)
                                                                             tCAATGACTGGCAGCAACACTGCAGCGCAGCTGCGTGATGAACATTCCTGGCGTTACGACCATCCCGGTGACGCTATCTACGCGCCGTTGTTGTTAAaac                   <  1:253708/100‑1 (MQ=255)
                                                                             tCAATGACTGGCAGCAACACTGCAGCGCAGCTGCGTGATGAACATTCCTGGCGTTACGACCATCCCGGTGACGCTATCTACGCGCCGTTGTTGTTAAaac                   <  1:538877/100‑1 (MQ=255)
                                                                              cAATGACTGGCAGCAACACTGCAGCGCAGCTGCGTGATGAACATTCCTGGCGTTACGACCATCCCGGTGACGCTATCTACGCGCCGTTGTTGTTAAaaca                  <  2:805694/100‑1 (MQ=255)
                                                                               aaTGACTGGCAGCAACACTGCAGCGCAGCTGCGTGATGAACATTCCTGGCGTTACGACCATCCCGGTGACGCTATCTACGCGCCGTTGTTGTTAAaacaa                 >  2:1514626/1‑100 (MQ=255)
                                                                               aaTGACTGGCAGCAACACTGCAGCGCAGCTGCGTGATGAACATTCCTGGCGTTACGACCATCCCGGTGACGCTATCTACGCGCCGTTGTTGTTAAaacaa                 >  2:2609242/1‑100 (MQ=255)
                                                                                aTGACTGGCAGCAACACTGCAGCGCAGCTGCGTGATGAACATTCCTGGCGTTACGACCATCCCGGTGACGCTATCTACGCGCCGTTGTTGTTAAaacaac                <  1:847824/100‑1 (MQ=255)
                                                                                 tGACTGGCAGCAACACTGCAGCGCAGCTGCGTGATGAACATTCCTGGCGTTACGACCATCCCGGTGACGCTATCTACGCGCCGTTGTTGTTAAAACAACt               <  2:2239864/100‑1 (MQ=255)
                                                                                  gACTGGCAGCAACACTGCAGCGCAGCTGCGTGATGAACATTCCTGGCGTTACGACCATCCCGGTGACGCTATCTACGCGCCGTTGTTGTTAAAACAACTg              <  2:485756/100‑1 (MQ=255)
                                                                                   aCTGGCAGCAACACTGCAGCGCAGCTGCGTGATGATCATTCCTGGCGTTACGAACATCCCGGTGACGCTATCTACGCGCCGTTGTTGTTAAAACAACt               <  2:1339633/98‑1 (MQ=255)
                                                                                     tGGCAGCAACACTGCAGCGCAGCTGCGTGATGAACATTCCTGGCGTTACGACCATCCCGGTGACGCTATCTACGCGCCGTTGTTGTTAAAACAACTGTCg           <  2:2362844/100‑1 (MQ=255)
                                                                                      ggCAGCAACACTGCAGCGCAGCTGCGTGATGAACATTCCTGGCGTTACGACCATCCCGGTGACGCTATCTACGCGCCGTTGTTGTTAAAACAACTGTCgg          >  1:2304826/1‑100 (MQ=255)
                                                                                      ggCAGCAACACTGCAGCGCAGCTGCGTGATGAACATTCCTGGCGTTACGACCATCCCGGTGACGCTATCTACGCGCCGTTGTTGTTAAAACAACTGTCgg          <  2:628386/100‑1 (MQ=255)
                                                                                      ggCAGCAACACTGCAGCGCAGCTGCGTGATGAACATTCCTGGCGTTACGACCATCCCGGTGACGCTATCTACGCGCCGTTGTTGTTAAAACAACTGTCgg          >  1:1449907/1‑100 (MQ=255)
                                                                                       gcagcaACACTGCAGCGCAGCTGCGTGATGAACATTCCTGGCGTTACGACCATCCCGGTGACGCTATCTACGCGCCGTTGTTGTTAAAACAACTGTCGGa         >  1:2368737/1‑100 (MQ=255)
                                                                                        cagcaACACTGCAGCGCAGCTGCGTGATGAACATTCCTGGCGTTACGACCATCCCGGTGACGCTATCTACGCGCCGTTGTTGTTAAAACAACTGTCGGAt        >  2:1762409/1‑100 (MQ=255)
                                                                                         agcaACACTGCAGCGCAGCTGCGTGATGAACATTCCTGGCGTTACGACCATCCCGGTGACGCTATCTACGCGCCGTTGTTGTTAAAACAACTGTCGGATc       <  2:315279/100‑1 (MQ=255)
                                                                                          gcaACACTGCAGCGCAGCTGCGTGATGAACATTCCTGGCGTTACGACCATCCCGGTGACGCTATCTACGCGCCGTTGTTGTTAAAACAACTGTCGGATCg      <  1:1848439/100‑1 (MQ=255)
                                                                                          gcaACACTGCAGCGCAGCTGCGTGATGAACATTCCTGGCGTTACGACCATCCCGGTGACGCTATCTACGCGCCGTTGTTGTTAAAACAACTGTCGGATCg      <  2:2660165/100‑1 (MQ=255)
                                                                                           caACACTGCAGCGCAGCTGCGTGATGAACATTCCTGGCGTTACGACCATCCCGGTGACGCTATCTACGCGCCGTTGTTGTTAAAACAACTGTCGGATCGt     <  1:2419424/100‑1 (MQ=255)
                                                                                           caACACTGCAGCGCAGCTGCGTGATGAACATTCCTGGCGTTACGACCATCCCGGTGACGCTATCTACGCGCCGTTGTTGTTAAAACAACTGTCGGATCGt     >  1:2472829/1‑100 (MQ=255)
                                                                                            aaCACTGCAGCGCAGCTGCGTGATGAACATTCCTGGCGTTACGACCATCCCGGTGACGCTATCTACGCGCCGTTGTTGTTAAAACAACTGTCGGATCGTa    >  1:245016/1‑100 (MQ=255)
                                                                                              cacTGCAGCGCAGCTGCGTGATGAACATTCCTGGCGTTACGACCATCCCGGTGACGCTATCTACGCGCCGTTGTTGTTAAAACAACTGTCGGATCGTaaa  >  2:284505/1‑100 (MQ=255)
                                                                                                    |                                                                                             
AACAAGCTGCGTCAGGCACATGTGGCATTACAAGGTGATTTAAATGCTCTGTTACCAGCATTACAGCAGCCGTTAAATCAATGACTGGCAGCAACACTGC‑GCGCAGCTGCGTGATGAACATTCCTGGCGTTACGACCATCCCGGTGACGCTATCTACGCGCCGTTGTTGTTAAAACAACTGTCGGATCGTAAA  >  NC_000913/3951460‑3951652

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A