Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A11 F212 I0 R1
|
246 |
122.0 |
6097616 |
93.1% |
5676880 |
99.8 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
freq |
annotation |
gene |
description |
| RA |
NC_000913 |
1,580,094 |
C→T |
100% |
D233N (GAT→AAT) |
ydeM ← |
putative YdeN‑specific sulfatase‑maturating enzyme |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | NC_000913 | 1,580,094 | 0 | C | T | 98.9%
| 243.5
/ ‑5.8
| 74 | D233N (GAT→AAT) | ydeM | putative YdeN‑specific sulfatase‑maturating enzyme |
| Reads supporting (aligned to +/- strand): ref base C (1/0); new base T (36/37); total (37/37) |
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 |
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.99e-01 |
TCCTTGCAGGACTCCTGGAAAATACAACTGGTATGCCCATTCCCCAAAAAACGGCTGACAAAGCTTTCAAACTGACGGATGAAAATTTCACCCACATCGTTTTTAACCCATTGCATAAAAATGGTTGACATAAACTTGCCATAAGCCGTGGGAGGCACAGAAAAATCAATGATACGGAATGTGTTCTCACTAT > NC_000913/1579997‑1580189
|
tCCTTGCAGGACTCCTGGAAAATACAACTGGTATGCCCATTCCCCAAAAAACGGCTGACAAAGCTTTCAAACTGACGGATGAAAATTTCACCCACATTGt > 2:1443379/1‑100 (MQ=255)
ccTTGCAGGACTCCTGGAAAATACAACTGGTATGCCCATTCCCCAAAAAACGGCTGACAAAGCTTTCAAACTGACGGATGAAAATTTCACCCACATTGtt > 1:2974661/1‑100 (MQ=255)
cTTGCAGGACTCCTGGAAAATACAACTGGTATGCCCATTCCCCAAAAAACGGCTGACAAAGCTTTCAAACTGACGGATGAAAATTTCACCCACATTGttt < 1:203451/100‑1 (MQ=255)
cTTGCAGGACTCCTGGAAAATACAACTGGTATGCCCATTCCCCAAAAAACGGCTGACAAAGCTTTCAAACTGACGGATGAAAATTTCACCCACATTGttt < 2:542363/100‑1 (MQ=255)
ttGCAGGACTCCTGGAAAATACAACTGGTATGCCCATTCCCCAAAAAACGGCTGACAAAGCTTTCAAACTGACGGATGAAAATTTCACCCACATTGtttt < 1:2105197/100‑1 (MQ=255)
ttGCAGGACTCCTGGAAAATACAACTGGTATGCCCATTCCCCAAAAAACGGCTGACAAAGCTTTCAAACTGACGGATGAAAATTTCACCCACATTGtttt < 2:2313673/100‑1 (MQ=255)
ttGCAGGACTCCTGGAAAATACAACTGGTATGCCCATTCCCCAAAAAACGGCTGACAAAGCTTTCAAACTGACGGATGAAAATTTCACCCACATTGtttt < 1:733417/100‑1 (MQ=255)
tggatgtctgcTGGAAAATACAACTGGTATGCCGATTCTCCAAAAAACGGCTGACAAAGCTTTCAAACTGACGCATGAATATTTCACCCACATTGttttt < 2:2337976/90‑1 (MQ=255)
aGGACTCCTGGAAAATACAACTGGTATGCCCATTCCCCAAAAAACGGCTGACAAAGCTTTCAAACTGACGGATGAAAATTTCACCCACATTGTTTTTAAc < 1:2089806/100‑1 (MQ=255)
cTCCTGGAAAATACAACTGGTATGCCCATTCCCCAAAAAACGGCTGACAAAGCTTTCAAACTGACGGATGAAAATTTCACCCACATTGTTTTTAACCCAt < 2:2005749/100‑1 (MQ=255)
tCCTGGAAAATACAACTGGTATGCCCATTCCCCAAAAAACGGCTGACAAAGCTTTCAAACTGACGGATGAAAATTTCACCCACATTGTTTTTAACCCAtt < 1:1392811/100‑1 (MQ=255)
tCCTGGAAAATACAACTAGTATGCCCATTTACCAAAAAACGGCTGACAAAGCTTTCAAACTGACGGATGAAAATTTCATCCACATTGTTTTTAACCCa < 2:563574/98‑1 (MQ=255)
ccTGGAAAATACAACTGGTATGTCCATTCCCCAAAAAACGGCTGACAAAGCTTTCAAACTGACGGATGAAAATTTCACCCACATTGTTTTTAACCCATTg > 1:912877/1‑100 (MQ=255)
cTGTAAAATACAACTGGTATGCCCATTCCCCAAAAAACGGCTGACAAAGCTTTCAAACTGACGGATGAAAATTTCACCCACATTGTTTTTAACCCATTg < 2:2669234/99‑1 (MQ=255)
cTGGGAAATACAACAGGTATGCCCATGCCCCAAAAAACGGCTGACAACGCTGTCAAACTGACTGATAAAAAAATCACCCACATCGTTTTTAACCCttt > 2:241380/1‑98 (MQ=255)
cTGGAAAATACAACTGGTATGCCCATTCCCCAAAAAACGGCTGACAAAGCTTTCAAACTGACGGATGAAAATTTCACCCACATTGTTTTTAACCCATTGc > 1:1221639/1‑100 (MQ=255)
tGGAAAATACAACTGGTATGCCCATTCCCCAAAAAACGGCTGACAAAGCTTTCAAACTGACGGATGAAAATTTCACCCACATTGTTTTTAACCCATTGCa > 1:2607452/1‑100 (MQ=255)
ggAAAATACAACTGGTATGCCCATTCCCCAAAAAACGGCTGACAAAGCTTTCAAACTGACGGATGAAAATTTCACCCACATTGTTTTTAACCCATTGCAt > 1:197111/1‑100 (MQ=255)
aaTACAACTTGTATGCCTATTCCCCAAAAAACGGCTGACAATACTTTCAAACTGACTGATGAAAATTTCACCCACATTGTTTTTAACCCATTGCATaaaa < 2:1298950/100‑1 (MQ=255)
aCAACTGGTATGCCCATTCCCCAAAAAACGTCTGACAAAGCTTTCAAACTGACGGATGAAAATTTCACCCACATTGTTTTTAACCCATAGCATAAAAATg < 2:2839135/100‑1 (MQ=255)
cAACTGGTATGCCCATTCCCCAAAAAACGGCTGACAAAGCTTTCAAACTGACGGATGAAAATTTCACACACATTGTTTTTAACCAATTGAATAAAAATg > 1:608831/1‑99 (MQ=255)
aCTGGTATGCCCATTCCCCAAAAAACGGCTGACAAAGCTTTCAAACTGACGGATGAAAATTTCACCCACATTGTTTTTAACCCATTGCATAAAAATGGtt > 1:1905883/1‑100 (MQ=255)
aCTGGTATGCCCATTCCCCAAAAAACGGCTGACAAAGCTTTCAAACTGACGGATGAAAATTTCACCCACATTGTTTTTAACCCATTGCATAAAAATGGtt > 2:1192491/1‑100 (MQ=255)
aCTGGTATGCCCATTCCCCAAAAAACGGCTGACAAAGCTTTCAAACTGACGGATGAAAATTTCACCCACATTGTTTTTAACCCATTGCATAAAAATGGtt > 2:2750718/1‑100 (MQ=255)
cTGGTATGCCCATTCCCCAAAAAACGGCTGACAAAGCTTTCAAACTGACGGATGAAAATTTCACCCACATTGTTTTTAACCCATTGCATAAAAATGGTTg < 2:664823/100‑1 (MQ=255)
cTGGTATGCCCATTCCCCAAAAAACGGCTGACAAAGCTTTCAAACTGACGGATGAAAATTTCACCCACATTGTTTTTAACCCATTGCATAAAAATGGTTg < 1:1761037/100‑1 (MQ=255)
cTGGTATGCCCATTCCCCAAAAAACGGCTGACAAAGCTTTCAAACTGACGGATGAAAATTTCACCCACATTGTTTTTAACCCATTGCATAAAAATGGTTg < 1:1761221/100‑1 (MQ=255)
ggTATGCCCATTCCCCAAAAAACGGCTGACAAAGCTTTCAAACTGACGGATGAAAATTTCACCCACATTGTTTTTAACCCATTGCATAAAAATGGTTGAc > 1:834138/1‑100 (MQ=255)
gTATGCCTATTCCCCAAAAAACGGCTGACAAAGCTTTCAAACTGACGGATGAAAATTTCACCCACATTGTTTTTAACCCATTGCATAAAAATGGTTGAc < 1:1347929/99‑1 (MQ=255)
gTATGCCCATTCCCCAAAAAACGGCTGACAAAGCTTTCAAACTGACGGATGAAAATTTCACCCACATTGTTTTTAACCCATTGCATAAAAATGGTTGACa < 1:2192854/100‑1 (MQ=255)
gTATGCCCATTCCCCAAAAAACGGCTGACAAAGCTTTCAAACTGACGGATGAAAATTTCACCCACATTGTTTTTAACCCATTGCATAAAAATGGTTGACa > 1:568467/1‑100 (MQ=255)
ttccgatctCCCCAAAAAACGGCTGACAAAGCTTTCAAACTGACGGATGAAAATTTCACCCACATTGTTTTTAACCCATTGCATAAAAATGGTTGACATa < 1:2471026/92‑1 (MQ=255)
gCCCATTCCCCAAAAAACGGCTGACAAAGCTTTCAAACTGACGGATGAAAATTTCACCCACATTGTTTTTAACCCATTGCATAAAAATGGTTGACATaaa > 1:1937080/1‑100 (MQ=255)
aTTCCCCAAAAAACGGCTGACAAAGCTTTCAAACTGACGGATGAAAATTTCACCCACATTGTTTTTAACCCATTGCATAAAAATGGTTGACATAAACTTg < 1:176022/100‑1 (MQ=255)
tCCCCAAAAAACGGCTGACAAAGCTTTCAAACTGACGGATGAAAATTTCACCCACATTGTTTTTAACCCATTGCATAAAAATGGTTGACATAAACTTGcc < 1:2795935/100‑1 (MQ=255)
ccccAAAAAACGGCTGACAAAGCTTTCAAACTGACGGATGAAAATTTCACCCACATTGTTTTTAACCCATTGCATAAAAATGGTTGACATaagatcggaa > 2:2470997/1‑92 (MQ=255)
ccccAAAAAACGGCTGACAAAGCTTTCAAACTGACGGATGAAAATTTCACCCACATTGTTTTTAACCCATTGCATAAAAATGGTTGACATAAACTTGCCa < 2:280279/100‑1 (MQ=255)
ccccAAAAAACGGCTGACAAAGCTTTCAAACTGACGGATGAAAATTTCACCCACATTGTTTTTAACCCATTGCATAAAAATGGTTGACATAAACTTGCCa > 1:1689074/1‑100 (MQ=255)
cccAAAAAACGGCTGACAAAGCTTTCAAACTGACGGATGAAAATTTCACCCACATTGTTTTTAACCCATTGCATAAAAATGGTTGACATAAACTTGCCAt > 1:2844603/1‑100 (MQ=255)
cccAAAAAACGGCTGACAAAGCTTTCAAACTGACGGATGAAAATTTCACCCACATTGTTTTTAACCCATTGCATAAAAATGGTTGACATAAACTTGCCAt > 1:1636217/1‑100 (MQ=255)
cAAAAAACTGCTGACAAAGCTTTCAAGCTGACGTATGAAAATTTCACCCACATTGTTTTTAACCCCTTGCATAAACATGGTTGACATAAACGTGCCAt < 2:2643472/98‑1 (MQ=255)
aaaaaCGGCTGACAAAGCTTTCAAACTGACGGATGAAAATTTCACCCACATTGTTTTTAACCCATTGCATAAAAATGGTTGACATAAACTTGCCATAAGc < 2:1122046/100‑1 (MQ=255)
cGGCTGACAAAGCTTTCAAACTGACGGATGAAAATTTCACCCACATTGTTTTTAACCCATTGCATAAAAATGGTTGACATAAACTTGCCATAAGCCGTgg > 2:2433711/1‑100 (MQ=255)
ggCTGACAAAGCTTTCAAACTGACGGATGAAAATTTCACCCACATTGTTTTTAACCCATTGCATAAAAATGGTTGACATAATCTTGCCATAAGCCGTggg < 2:1483362/100‑1 (MQ=255)
ggCTGACAAAGCTTTCAAACTGACGGATGAAAATTTCACCCACATTGTTTTTAACCCATTGCATAAAAATGGTTGACATAAACTTGCCATAAGCCGTggg < 1:2685639/100‑1 (MQ=255)
ggCTGACAAAGCTTTCAAACTGACGGATGAAAATTTCACCCACATTGTTTTTAACCCATTGCATAAAAATGGTTGACATAAACTTGCCATAAGCCGTgg > 2:2698660/1‑99 (MQ=255)
ggCTGACAAAGCTTTCAAACTGACGGATGAAAATTTCACCCACATTGTTTTTAACCCATTGCATAAAAATGGTTGACATAAACTTGCCATAAGCCGTgg < 2:1247445/99‑1 (MQ=255)
ggCTGACAAAGCTTTCAAACTGACGGATGAAAATTTCACCCACATTGTTTTTAACCCATTGCATAAAAATGGTTGACACAAACTTGCCATAAGCCGTgg > 2:2698842/1‑99 (MQ=255)
cTGACAAAGCTTTCAAACTGACGGATGAAAATTTCACCCACATTGTTTTTAACCCATTGCATAAAAATGGTTGACATAAACTTGCCATAAGCCGTGGGAg > 2:2312305/1‑100 (MQ=255)
tGACAAAGCTTTCAAACTGACGGATGAAAATTTCATCCATATTGTTTTTAATCCATTGCATAAAAATGGTTGACATAAACTTGCCATAAGCCGTGGGAgg < 1:776782/100‑1 (MQ=255)
gACAAAGCTTTCAAACTGACGGATGAAAATTTCACCCACATTGTTTTTAACCCATTGCATAAAAATGGTTGACATAAACTTGCCATAAGCCGTGGGAGGc < 2:453334/100‑1 (MQ=255)
gACAAAGCTTTCAAAATGACGGATGAAAATTTCACCCACATTGTTTTTAACCCATTGCATAAAAATGGTTGACATAAACTTGCCATAAGCCGTGGGAGGc > 2:595660/1‑100 (MQ=255)
cAAAGCTTTCAAACTGACGGATGAAAATTTCACCCACATTGTTTTTAACCCATTGCATAAAAATGGTTGACATAAACTTGCCATAAGCCGTGGGAGGcac > 1:1487174/1‑100 (MQ=255)
aaaGCTTTCAAACTGACGGATGAAAATTTCACCCACATTGTTTTTAACCCATTGCATAAAAATGGTTGACATAAACTTGCCATAAGCTGTGGGAGGcaca > 2:1050639/1‑100 (MQ=255)
aaGCTTTCAAACTGACGGATGAAAATTTCACCCACATTGTTTTTAACCCATTGCATAAAAATGGTTGACATAAACTTGCCATAAGCCGTGGGAGGcaaa > 2:1783004/1‑97 (MQ=255)
aaGCTTTCAAACTGACGGATGAAAATTTCACCCACATTGTTTTTAACCCATTGCATAAAAATGGTTGACATAAACTTGCCATAAGCCGTGGGAGGCACAg < 1:1932492/100‑1 (MQ=255)
aaGCTTTCAAACTGACGGATGAAAATTTCACCCACATTGTTTTTAACCCATTGCATAAAAATGGTTGACATAAACTTGCCATAAGCCGTGGGAGGCACAg > 2:2176097/1‑100 (MQ=255)
cAAACTGACGGATGAAATTTTTATCCACTTTTTTTTTAACCCATTGCATAAAAATGGTTGACATAAACTTGCCATAAGCCGTGGGAGGCACAGAAAAATc < 1:465307/100‑1 (MQ=255)
aaaCTGACGGATGAAAATTTCACCCACATTGATTTTAACCCATTGCATAAAAATGGTTGACATAAACTTGCCATAAGCCGTGGGAGGCACAGAAAAAt > 2:1310279/1‑98 (MQ=255)
aCTGACGGATGAAAATTTCACCCACATTGTTTTTAACCCATTGCATAAAAATGGTTGACATAAACTTGCCATAAGCCGTGGGAGGCACAGAAAAATCAAt < 1:1172386/100‑1 (MQ=255)
aCTGACGGATGAAAATTTCACCCACATTGTTTTTAACCCATTGCATAAAAATGGTTGACATAAACTTGCCATAAGCCGTGGGAGGCACAGAAAAATCAAt < 1:2750750/100‑1 (MQ=255)
aCTGACGGATGAAAATTTCACCCACATTGTTTTTAACCCATTGCATAAAAATGGTTGACATAAACTTGCCATAAGCCGTGGGAGGCACAGAAAAATCAAt < 1:1287981/100‑1 (MQ=255)
tGACGGATGAAAATTTCACCCACATTGTTTTTAACCCATTGCATAAAAATGGTTGACATAAACTTGCCATAAGCCGTGGGAGGCACAGAAAAATCAATGa > 1:2089613/1‑100 (MQ=255)
gACGGATGAAAATTTCACCCACATTGTTTTTAACCCATTGCATAAAAATGGTTGACATAAACTTGCCATAAGCCGTGGGAGGCACAGAAAAATCAATGAt > 1:1974744/1‑100 (MQ=255)
gACGGATGAAAATTTCACCCACATTGTTTTTAACCCATTGCATAAAAATGGTTGACATAAACTTGCCATAAGCCGTGGGAGGCACAGAAAAATCAATGAt > 1:1711215/1‑100 (MQ=255)
gACGGATGAAAATTTCACCCACATTGTTTTTAACCCATTGCATAAAAATGGTTGACATAAACTTGCCATAAGCCGTGGGAGGCACAGAAAAATCAATGAt > 1:1709166/1‑100 (MQ=255)
gACGGATGAAAATTTCACCCACATTGTTTTTAACCCATTGCATAAAAATGGTTGACATAAACTTACCATAAGCCATAGGAGGCACAGAAAAATCAATGAt > 1:1041246/1‑100 (MQ=255)
gATGAAAATTTCACCCACATTGTTTTTAACCCATTGCATAAAAATGGTTGACATAAACTTGCCATAAGCCGTGGGAGGCACAGAAAAATCAATGATACgg > 1:384121/1‑100 (MQ=255)
gATGAAAATTTCACCCACATTGTTTTTAACCCATTGCATAAAAATGGTTGACATAAACTTGCCATAAGCCGTGGGAGGCACAGAAAAATCAATGATACg < 1:2659599/99‑1 (MQ=255)
gAAAATTTCACCCACATTGTTTTTAACCCATTGCATAAAAATGGTTGACATAAACTTTCCATAAGCCGTAGGAGGCACAGAAAAATCAATGATACGGAAt > 1:684433/1‑100 (MQ=255)
cACCCACATTGTTTTTAACCCATTGCATAAAAATGGTTGACATAAACTTGCCATAAGCCGTGGGAGGCACAGAAAAATCAATGATACGGAATGTGTtctc < 2:2197822/100‑1 (MQ=255)
ccACATTGTTTTTAACCCATTGCATAAAAATGGTTGACATAAACTTGCCATAAGCCGTGGGAGGCACAGAAAAATCAATGATACGGAATGTGTTCTCACt > 1:2934017/1‑100 (MQ=255)
acaTTGTTTTTAACCCATTGCATAAAAATGGTTGACATAAACTTGCCATAAGCCGTGGGAGGCACAGAAAAATCAATGATACGGAATGTGTTCTCACTAt < 1:906949/100‑1 (MQ=255)
caTTGTTTTTAACCCATTGCATAAAAATGGTTGACATAAACTTGCCATAAGCCGTGGGAGGCACAGAAAAATCAATGATACGGAATGTGTTCTCACTAt > 1:904855/1‑99 (MQ=255)
|
TCCTTGCAGGACTCCTGGAAAATACAACTGGTATGCCCATTCCCCAAAAAACGGCTGACAAAGCTTTCAAACTGACGGATGAAAATTTCACCCACATCGTTTTTAACCCATTGCATAAAAATGGTTGACATAAACTTGCCATAAGCCGTGGGAGGCACAGAAAAATCAATGATACGGAATGTGTTCTCACTAT > NC_000913/1579997‑1580189
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
