Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A12 F56 I1 R1
|
165 |
53.8 |
2611523 |
96.4% |
2517508 |
99.9 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
annotation |
gene |
description |
| RA |
NC_000913 |
1,580,094 |
C→T |
D233N (GAT→AAT) |
ydeM ← |
putative YdeN‑specific sulfatase‑maturating enzyme |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | NC_000913 | 1,580,094 | 0 | C | T | 96.2%
| 169.8
/ ‑2.0
| 52 | D233N (GAT→AAT) | ydeM | putative YdeN‑specific sulfatase‑maturating enzyme |
| Reads supporting (aligned to +/- strand): ref base C (1/1); new base T (27/23); total (28/24) |
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 |
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.86e-01 |
CCTTGCAGGACTCCTGGAAAATACAACTGGTATGCCCATTCCCCAAAAAACGGCTGACAAAGCTTTCAAACTGACGGATGAAAATTTCACCCACATCGTTTTTAACCCATTGCATAAAAATGGTTGACATAAACTTGCCATAAGCCGTGGGAGGCACAGAAAAATCAATGATACGGAATGTGTTCTCACTATG > NC_000913/1579998‑1580190
|
ccTTGCAGGACTCCTGGAAAATACAACTGGTATGCCCATTCCCCAAAAAACGGCTGACAAAGCTTTCAAACTGACGGATGAAAATTTCACCCACATTGtt > 2:576449/1‑100 (MQ=255)
cTTGCAGGACTCCTGGAAAATACAACTGGTATGCCCATTCCCCAAAAAACGGCTGACAAAGCTTTCAAACTGACGGATGAAAATTTCACCCACATTGttt < 1:1225689/100‑1 (MQ=255)
cAGGACTCCTGGAAAATACAACTGGTATGCCCATTCCCCAAAAAACGGCTGACAAAGCTTTCAAACTGACGGATGAAAATTTCACCCACATTGTTTTTaa > 2:455798/1‑100 (MQ=255)
ccTGGAAAATACAACTGGTATGCCCATTCCCCAAAAAACGGCTGACAAAGCTTTCAAACTGACGGATGAAAATTTCACCCACATTGTTTTTAACCCATTg < 2:1253036/100‑1 (MQ=255)
cTGGAAAATACAACTGGTATGCCCATTCCCCAAAAAACGGCTGACAAAGCTTTCAAACTGACGGATGAAAATTTCTCCCACATTGTTTTTAACCCATTGc < 1:561766/100‑1 (MQ=255)
ggAAAATACAACTGGTATGCCCATTCCCCAAAAAACGGCTGACAAAGCTTTCAAACTGACGGATGAAAATTTCACCCACATTGTTTTTAACCCATTGCAt > 1:501754/1‑100 (MQ=255)
ggAAAATACAACTGGTATGCCCATTCCCCAAAAAACGGCTGACAAAGCTTTCAAACTGACGGATGAAAATTTCACCCACATTGTTTTTAACCCATTGCAt > 2:219404/1‑100 (MQ=255)
ggAAAATACAACTGGTATGCCCATTCCCCAAAAAACGGCTGACAAAGCTTTCAAACTGACGGATGAAAATTTCACCCACATTGTTTTTAACCCATTGCAt > 1:402679/1‑100 (MQ=255)
gAAAATACAACTGGTATGCCCATTCCCCAAAAAACGGCTGACAAAGCTTTCAAACTGACGGATGAAAATTTCACCCACATTGTTTTTAACCCATTGCATa < 1:1304416/100‑1 (MQ=255)
tACAACTGGTATGCCCATTCCCCAAAAAACGGCTGACAAAGCTTTCAAACTGACGGATGAAAATTTCACCCACATTGTTTTTAACCCATTGCATAAAAAt > 2:1256550/1‑100 (MQ=255)
aCAACTGGTATGCCCATTCCCCAAAAAACGGCTGACAAAGCTTTCAAACTGACGGATGAAAATTTCACCCACATTGTTTTTAACCCATTGCATAAAAATg > 1:138120/1‑100 (MQ=255)
aaCTGGTATGCCCATTCCCCAAAAAACGGCTGACAAAGCTTTCAAACTGACGGATGAAAATTTCACCCACATTGTTTTTAACCCATTGCATAAAAATGGt < 2:599668/100‑1 (MQ=255)
aCTGGTATGCCCATTCCCCAAAAAACGGCTGACAAAGCTTTCAAACTGACGGATGAAAATTTCTCCCACATTGTTTTTAACCCATTGCATAAAAATg < 1:631693/97‑1 (MQ=255)
cTGGTATGCCCATTCCCCAAAAAACGGCTGACAAAGCTTTCAAACTGACGGATGAAAATTTCACCCACATTGTTTTTATCCCATTGCATAAAAATGGtt < 1:466037/99‑1 (MQ=255)
cTGGTATGCCCATTCCCCAAAAAACGGCTGACAAAGCTTTCAAACTGACGGATGAAAATTTCACCCACATTGTTTTTAACCCATTGCATAAAAATGGTTg > 1:754345/1‑100 (MQ=255)
cTGGTATGCCCATTCCCCAAAAAACGGCTGACAAAGCTTTCAAACTGACGGATGAAAATTTCACCCACATCGTTTTTAACCCATTGCATAAAAATGGTTg < 2:403913/100‑1 (MQ=255)
gTATGCCCATTCCCCAAAAAACGGCTGACAAAGCTTTCAAACTGACGGATGAAAATTTCACCCACATTGTTTTTAACCCATTGCATAAAAATGGTTGACa > 1:1193682/1‑100 (MQ=255)
tATGCCCATTCCCCAAAAAACGGCTGACAAAGCTTTCAAACTGACGGATGAAAATTTCACCCACATTGTTTTTAACCCATTGCATAAAAATGGTTGACAt > 2:190602/1‑100 (MQ=255)
cccATTCCCCAAAAAACGGCTGACAAAGCTTTCAAACTGACGGATGAAAATTTCACCCACATTGTTTTTAACCCATTGCATAAAAATGGTTGACATAAAc > 2:621427/1‑100 (MQ=255)
ccATTCCCCAAAAAACGGCTGACAAAGCTTTCAAACTGACGGATGAAAATTTCACCCACATTGTTTTTAACCCATTGCATAAAAATGGTTGACATAAACt > 2:1156245/1‑100 (MQ=255)
cATTCCCCAAAAAACGGCTGACAAAGCTTTCAAACTGACGGATGAAAATTTCACCCACATTGTTTTTAACCCATTGCATAAAAATGGTTGACATAAACtt < 1:612687/100‑1 (MQ=255)
cccAAAAAACGGCTGACAAAGCTTTCAAACTGACGGATGAAAATTTCACCCACATTGTTTTTAACCCATTGCATAAAAATGGTTGACATAAACTTGCCAt < 2:1183021/100‑1 (MQ=255)
cccAAAAAACGGCTGACAAAGCTTTCAAACTGACGGATGAAAATTTCACCCACATCGTTTTTAACCCATTGCATAAAAATGGTTGACATAAACTTGCCAt > 2:829934/1‑100 (MQ=255)
ccAAAAAACGGCTGACAAAGCTTTCAAACTGACGGATGAAAATTTCACCCACATTGTTTTTAACCCATTGCAAAAAAATGGATGACAAAAACTTACCATa > 1:444128/1‑100 (MQ=255)
aaaaCGGCTGACAAAGCTTTCAAACTGACGGATGAAAATTTCACCCACATTGTTTTTAACCCATTGCATAAAAATGGTTGACATAAACTTGCCATAAGcc < 1:232998/100‑1 (MQ=255)
gCTGACAAAGCTTTCAAACTGACGGATGAAAATTTCACCCACATTGTTTTTAACCCATTGCATAAAAATGGTTGACATAAACTTGCCATAAGCCGTGGGa < 2:331301/100‑1 (MQ=255)
tGACAAAGCTTTCAAACTGACGGATGAAAATTTCACCCACATTGTCTTTAACCCATTGCATAAAAATGGTTGACATAAACTTGCCATAAGCCGTGGGAgg < 1:52971/100‑1 (MQ=255)
tccgatcTTTCAAACTGACGGATGAAAATTTCACCCACATTGTTTTTAACCCATTGCATAAAAATGGTTGACATAAACTTGCCATAAGCCGTGGGAGGca < 2:124497/94‑1 (MQ=255)
aaGCTTTCAAACTGACGGATGAAAATTTCACCCACATTGTTTTTAACCCATTGCATAAAAATGGTTGACATAAACTTGCCATAAGCCGTGGGAGGCACAg > 1:779311/1‑100 (MQ=255)
cTTTCAAACTGACGGATGAAAATTTCACCCACATTGTTTTTAACCCATTGCATAAAAATGGTTGACATAAACTTGCCATAAGCCGTGGGAGGCACAGaaa > 1:1201864/1‑100 (MQ=255)
ttCAAACTGACGGATGAAAATTTCACCCACATTGTTTTTAACCCATTGCATAAAAATGGTTGACATAAACTTGCCATAAGCCGTGGGAGGCACAGaaaaa < 1:767217/100‑1 (MQ=255)
ttCAAACTGACGGATGAAAATTTCACCCACATTGTTTTTAACCCATTGCATAAAAATGGTTGACATAAACTTGCCATAAGCCGTGGGAGGCACAGaaaaa > 1:45544/1‑100 (MQ=255)
ttCAAACTGACGGATGAAAATTTAACCCACATTGTTTTTAACCCATTGCATAAAAATGGTTGACATAAACTTGCCATAAGCCGTGGGAGGcaagatcgga > 1:124499/1‑92 (MQ=255)
tCAAACTGACGGATGAAAATTTCACCCACATTGTTTTTAACCCATTGCATAAAAATGGTTGACATAAACTTGCCATAAGCCGTGGGAGGCACAGaaaaan < 1:570025/100‑2 (MQ=255)
cAAACTGACGGATGAAAATTTCACCCACATTGTTTTTAACCCAGTGCATAAAAATGGTTGACATAAACTTGCCATAAGCCGTGGGAGGCACAGAAAAATc < 1:654512/100‑1 (MQ=255)
tGACGGATGAAAATTTGACCCACATTGTTTTTAACCCATTGCATAAAAATGGTTGACATAAGCTTGCCATAAGCCGTGGGAGGCACAGAAAAATCAAt < 2:444124/98‑1 (MQ=255)
cGGATGAAAATTTCACCCACATTGTTTTTAACCCATTGCATAAAAATGGTTGACATAAACTTGCCATAAGCCGTGGGAGGCACAGAAAAATCAATGATAc > 1:768942/1‑100 (MQ=255)
gATGAAAATTTCACCCACATTGTTTTTAACCCATTGCATAAAAATGGTTGACATAAACTTGCCATAAGCCGTGGGAGGCACAGAAAAATCAATGATACgg < 1:219406/100‑1 (MQ=255)
aTGAAAATTTCACCCACATTGTTTTTAACCCATTGCATAAAAATGGTTGACATAAACTTGCCATAAGCCGTGGGAGGCACAGAAAAATCAATGATACGGa < 2:138118/100‑1 (MQ=255)
tGAAAATTTCACCCACATTGTTTTTAACCCATTGCATAAAAATGGTTGACATAAGCTTGCCATAAGCCGTGGGAGGCACAGAAAAATCAATGATACGGaa > 1:954527/1‑100 (MQ=255)
tGAAAATTTCACCCACATTGTTTTTAACCCATTGCATAAAAATGGTTGACATAAACTTGCCATAAGCCGTGGGAGGCACAGAAAAATCAATGATACGGaa > 1:678725/1‑100 (MQ=255)
tGAAAATTTCACCCACATTGTTTTTAACCCATTGCATAAAAATGGTTGACATAAACTTGCCATAAGCCGTGGGAGGCACAGAAAAATCAATGATACGGaa > 1:1225126/1‑100 (MQ=255)
aaaaTTTCACCCACATTGTTTTTAACCCATTGCATAAAAATGGTTGACATAAACTTGCCATAAGCCGTGGGAGGCACAGAAAAATCAATGATACGGAAtg < 2:607413/100‑1 (MQ=255)
aTTTCACCCACATTGTTTTTAACCCATTGCATAAAAATGGTTGACATAAACTTGCCATAAGCCGTGGGAGGCACAGAAAAATCAATGATACGGAATgtgt > 1:333063/1‑100 (MQ=255)
ttCACCCACATTGTTTTTAACCCATTGCATAAAAATGGTTGACATAAACTTGCCATAAGCCGTGGGAGGCACAGAAAAATCAATGATACGGAATGTGTtc > 1:1195725/1‑100 (MQ=255)
cACCCACATTGTTTTTAACCCATTGCATAAAAATGGTTGACATAAACTTGCCATAAGCCGTGGGAGGCACAGAAAAATCAATGATACGGAATGTGTtctc > 2:470131/1‑100 (MQ=255)
cACCCACATTGTTTTTAACCCATTGCATAAAAATGGTTGACATAAACTTGCCATAAGCCGTGGGAGGCACAGAAAAATCAATGATACGGAATGTGTtctc > 1:626042/1‑100 (MQ=255)
cacaTTGTTTTTAACCCATTGCATAAAAATGGTTGACATAAACTTGCCATAAGCCGTGGGAGGCACAGAAAAATCAATGATACGGAATGTGTTCTCACTa > 1:109341/1‑100 (MQ=255)
acaTTGTTTTTAGCCCATTGCATAAAAATGGTTGACATAAACTTGCCATAAGCCGTGGGAGGCACAGAAAAATCAATGATACGGAATGTGTTCTCACTAt > 1:406848/1‑100 (MQ=255)
acaTTGATTTTAACCCAGTGCATAAAAATGGTTGACATAAACTTGCCATAAGCCGTGAAAAACACAAAAAAATCAATGATACGGAATGTGTTCTCACTa < 2:666013/99‑1 (MQ=255)
caTTGTTTTTAACCCATTGCATAAAAATGGTTGACATAAACTTGCCATAAGCCGTGGGAGGCACAGAAAAATCAATGATACGGAATGTGTTCTCACTATg < 2:259516/100‑1 (MQ=255)
caTTGTTTTTAACCCATTGCATAAAAATGGTTGACATAAACTTGCCATAAGCCGTGGGAGGCACAGAAAAATCAATGATACGGAATGTGTTCTCACTATg < 2:1255486/100‑1 (MQ=255)
|
CCTTGCAGGACTCCTGGAAAATACAACTGGTATGCCCATTCCCCAAAAAACGGCTGACAAAGCTTTCAAACTGACGGATGAAAATTTCACCCACATCGTTTTTAACCCATTGCATAAAAATGGTTGACATAAACTTGCCATAAGCCGTGGGAGGCACAGAAAAATCAATGATACGGAATGTGTTCTCACTATG > NC_000913/1579998‑1580190
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
