Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A11 F212 I0 R1 246 122.0 6097616 93.1% 5676880 99.8

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 4,098,512 C→A 100% R259R (CGC→CGA)  rhaS → transcriptional activator of rhaBAD and rhaT

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,098,5120CA99.2% 399.9 / ‑4.1 127R259R (CGC→CGA) rhaStranscriptional activator of rhaBAD and rhaT
Reads supporting (aligned to +/- strand):  ref base C (1/0);  new base A (67/59);  total (68/59)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.35e-01

Only 100 of 129 total aligned reads displayed.

AAGCCCGACATCTGCTACGCCACAGCGAGGCCAGCGTTACTGACATCGCCTATCGCTGTGGATTCAGCGACAGTAACCACTTTTCGACGCTTTTTCGCCGAGAGTTTAACTGGTCACCGCGTGATATTCGCCAGGGACGGGATGGCTTTCTGCAATAACGCGAATCTTCTCAACGTATTTGTACGCCATATTG  >  NC_000913/4098415‑4098607
                                                                                                 |                                                                                               
aaGCCCGACATCTGCTACGCCACAGCGAGGCCAGCGTTACTGACATCGCCTATCGCTGTGGATTCAGCGACAGTAACCACTTTTCGACGCTTTTTCGACg                                                                                               <  2:767121/100‑1 (MQ=255)
aaGCCCGACATCTGCTACGCCACAGCGAGGCCAGCGTTACTGACATCGCCTATCGCTGTGGATTCAGCGACAGTAACCACTTTTCGACGCTTTTTCGACg                                                                                               <  1:738297/100‑1 (MQ=255)
  gCCCGACATCTGCTACGCCACAGCGAGGCCAGCGTTACTGACATCGCATATCGCTGTGGATTAAGCGAAAGTAACCACATTTAGACGCTTTTTcgacga                                                                                              >  1:1764639/1‑99 (MQ=255)
   cccGACATCTGCTACGCCACAGCGAGGCCAGCGTTACTGACATCGCCTATCGCTGTGGATTCAGCGACAGTAACCACTTTTCGACGCTTTTTcgacgan                                                                                             <  2:1164380/99‑2 (MQ=255)
    ccGACATCTGCTACGCCACAGCGAGGCCAGCGTTACTGACATCGCCTATCGCTGTGGATTCAGCGACAGTAACCACTTTTCGACGCTTTTTCGACGagag                                                                                           <  2:2432029/100‑1 (MQ=255)
    ccGACATCTGCTACGCCACAGCGAGGCCAGCGTTACTGACATCGCCTATCGCTGTGGATTCAGCGACAGTAACCACTTTTCGACGCTTTTTCGACGagag                                                                                           <  2:2589981/100‑1 (MQ=255)
       aCATCTGCTACGCCACAGCGAGGCCAGCGTTACTGACATCGCCTATCGCTGTGGATTCCGCGACAGTAACCACTTTTCGACGCTTTTTCGACGAGAGttt                                                                                        <  2:1655261/100‑1 (MQ=255)
       aCATCTGCTACGCCACAGCGAGGCCAGCGTTACTGACATCGCCTATCGCTGTGGATTCAGCGACAGTAACCACTTTTCGACGCTTTTTCGACTAGAGttt                                                                                        <  1:1584564/100‑1 (MQ=255)
        cATCTGCTACGCCACAGCGAGGCCAGCGTTACTGACATCGCCTATCGCTGTGGATTCAGCGACAGTAACCACTTTTCGACGCTTTTTCGACGAGAGttt                                                                                        <  2:1203193/99‑1 (MQ=255)
        cATCTGCTACGCCACAGCGAGGCCAGCGTTACTGACATCGCCTATCGCTGTGGATTCAGCGACAGTAACCACTTTTCGACGCTTTTTCGACGAGAGTTTa                                                                                       <  2:2360406/100‑1 (MQ=255)
           cTGCTACGCCACAGCGAGGCCAGCGTTACTGACATCGCCTATCGCTGTGGATTCAGCGACAGTAACCACTTTTCGACGCTTTTTCGACGAGAGTTTAACt                                                                                    >  1:170002/1‑100 (MQ=255)
           cTGCTACGCCACAGCGAGGCCAGCGGTACTGACATCGCCTACCGCTGTGGATTCAGCGACAGTAACCACTTTTCGACGCTTTTTCGACGAGAGTTTAACt                                                                                    >  2:1646930/1‑100 (MQ=255)
            tGCTACGCCACAGCGAGGCCAGCGTTACTGACATCGCCTATCGCTGTGGATTCAGCGACAGTAACCACTTTTCGACGCTTTTTCGACGAGAGTTTAACt                                                                                    >  1:388487/1‑99 (MQ=255)
            tGCTACGCCACAGCGAGGCCAGCGTTACTGACATCGCCTATCGCTGTGGATTCAGCGACAGTAACCACTTTTCGACGCTTTTTCGACGAGAGTTTAACTg                                                                                   >  2:1666662/1‑100 (MQ=255)
             gCTACGCCACAGCGAGGCCAGCGTTACTGACATCGCCTATCGCTGTGGATTCAGCGACAGTAACCACTTTTCGACGCTTTTTCGACGAGAGTTTAACTgg                                                                                  >  1:690869/1‑100 (MQ=255)
             gCTACGCCACAGCGAGGCCAGCGTTACTGACATCGCCTATCGCTGTGGATTCAGCGACAGTAACCACTTTTCGACGCTTTTTCGACGAGAGTTTAACTgg                                                                                  >  2:2428391/1‑100 (MQ=255)
             gCTACGCCACAGCGAGGCCAGCGTTACTGACATCGCCTATCGCAGTGGATTCAGCGACAGTAACCACTTTTGGACGCTTTTTCGACGACAGTTTAAATgg                                                                                  >  2:1749845/1‑100 (MQ=255)
              cTACGCCACAGCGAGGCCAGCGTTACTGACATCGCCTATCGCTGTGGATTCAGCGACAGTAACCACTTTTCGACGCTTTTTCGCCGAGAGTTTAACTGGt                                                                                 >  1:1864325/1‑100 (MQ=255)
              cTACGCCACAGCGAGGCCAGCGTTACTGACATCGCCTATCGCTGTGGATTCAGCGACAGTAACCACTATTCAACGCTTTTTCGACGAGAGATTAACTGGt                                                                                 >  1:2377375/1‑100 (MQ=255)
               tACGCCACAGCGAGGCCAGCGTTACTGACATCGCCTATCGCTGTGGATTCAGCGACAGTAACCACTTTTCGACGCTTTTTCGACGAGAGTTTAACTGGTc                                                                                <  2:690865/100‑1 (MQ=255)
                 cGCCACAGCGAGGCCAGCGTTACTGACATCGCCTATCGCTGTGGATTCAGCGACAGTAACCACTTTTCGACGCTTTTTCGACGAGAGTTTAACTGGTCAc                                                                              <  2:2366012/100‑1 (MQ=255)
                  gCCACAGCGAGGCCAGCGTTACTGACATCGCCTATCGCTGTGGATTCAGCGACAGTAACCACTTTTCGACGCTTTTTCGACGAGAGTTTAACTGGTCAcc                                                                             >  1:2851297/1‑100 (MQ=255)
                   acacaGCGAGGCCAGCGTTACTGACATCGCCTATCGCTGTGGATTCAGCGACAGTAACCACTTTTCGACCCTTTTTCGACGGGAACTTAAATGGTCACcg                                                                            >  2:2711997/2‑100 (MQ=255)
                    cacaGCGAGGCCAGCGTTACTGACATCGCCTATCGCTGTGGATTCAGCGACAGTAACCACTTTTCGACGCTTTTTCGACGAGAGTTTAACTGGTCACcgc                                                                           >  2:1295545/1‑100 (MQ=255)
                      caGCGAGGCCAGCGTTACTGACATCGCCTATCGCTGTGGATTCAGCGACAGTAACCACTTTTCGACGCTTTTTCGACGAGAGTTTAACTGGTCACCGCGt                                                                         >  2:781654/1‑100 (MQ=255)
                       aGCGAGGCCAGCGTTACTGACATCGCCTATCGCTGTGGATTCAGCGACAGTAACCACTTTTCGACGCTTTTTCGACGAGAGTTTAACTGGTCACCGCGTg                                                                        <  2:1027117/100‑1 (MQ=255)
                        gCGAGGCCAGCGTTACTGACATCGCCTATCGCTGTGGATTCAGCGACAGTAACCACTTTTCGACGCTTTTTCGACGAGAGTTTAACTGGTCACCGCGTGa                                                                       <  1:137285/100‑1 (MQ=255)
                        gCGAGGCCAGCGTTACTGACATAGACTATCGAAGTGGATTAAGCGACAGTAACAACATAAAGAAGATTTTTCGACGAGAGATTAAATGGTAACagc                                                                           >  2:459010/1‑96 (MQ=255)
                         cGAGGCCAGCGTTACTGACATCGCCTATCGCTGTGGATTCAGCGACAGTAACCACTTTTCGACGCTTTTTCGACGAGAGTTTAACTGGTCACCGCGTGat                                                                      <  2:2761104/100‑1 (MQ=255)
                         cGAGGCCAGCGTTACTGACATCGCCTATCGCTGTGGATTCAGCGACAGTAACCACTTTTCGACGCTTTTTCGACGAGAGTTTAACTGGTCACCGCGTGat                                                                      >  1:1213015/1‑100 (MQ=255)
                         cGAGGCCAGCGTTACTGACATCGCCTATCGCTGTGGATTCAGCGACAGTAACCACTTTTCGACGCTTTTTCGACGAGAGTTTAACTGGTCACCGCGTGa                                                                       <  2:410801/99‑1 (MQ=255)
                            ggCCAGCGTTACTGACATCGCCTATCGCTGTGGATTCAGCGACAGTAACCACTTTTCGACGCTTTTTCGACGAGAGTTTAACTGGTCACCGCGTGATAtt                                                                   >  1:105388/1‑100 (MQ=255)
                            ggCCAGCGTTACTGACATCGCCTATCGCTGTGGATTCAGCGACAGTAACCACTTTTCGACGCTTTTTCGACGACAGTTTAACTAGTCACCGCGTGata                                                                     >  1:1676059/1‑98 (MQ=255)
                             gCCAGCGTTACTGACATCGCCTATCGCTGTGGATTCAGCGACAGTAACCACTTTTCGACGCTTTTTCGACGAGAGTTTAACTGGTCACCGCGTGATATTc                                                                  >  2:2467464/1‑100 (MQ=255)
                             gCCAGCGTTACTGACATCGCATATCGCTGTGGATTCAGCGACAGTAACCACTTTTCGACGCTTTTTCGACGAGAGTTTAACTGGTCACCGCGTGATATTc                                                                  >  2:1855966/1‑100 (MQ=255)
                              ccAGCGTTACTGACATCGCCTATCGCTGTGGATTCAGCGACAGTAACCACTTTTCGACGCTTTTTCGACGAGAGTTTAACTGGTCACCGCGTGATATTCg                                                                 <  1:2045797/100‑1 (MQ=255)
                               cAGCGTTACTGACATCGCCTATCGCTGTGGATTCAGCGACAGTAACCACTTTTCGACGCTTTTTCGACGAGAGTTTAACTGGTCACCGCGTGATATTCGc                                                                >  2:1085569/1‑100 (MQ=255)
                               cAGCGTTACTGACATCGCCTATCGCTGTGGATTCAGCGACAGTAACCACTTTTCGACGCTTTTTCGACGAGAGTTTAACTGGTCACCGCGTGATATTCGc                                                                >  2:1512837/1‑100 (MQ=255)
                               cAGCGTTACTGACATCGCATATCGCTGTGGATTCAGCGACGGTAACCACTTTTGTACGCTTTTTCGACGAGAGTTTAATTGGTCACCGCGTGATATTCGc                                                                <  2:856830/100‑1 (MQ=255)
                                aGCGTTACTGACATCGCCTATCGCTGTGGATTCAGCGACAGTAACCACTTTTCGACGCTTTTTCGACGAGAGTTTAACTGGTCACCGCGTGATATTCGcc                                                               <  1:1615562/100‑1 (MQ=255)
                                 gCGTTACTGACATCGCCTATCGCTGTGGATTCAGCGAAAGTAACCACTTTTCGACGCTTTTTCGACGAGAGTTTAACTGGTCACCGCGTGATATTCGcc                                                               <  1:717750/99‑1 (MQ=255)
                                   gTTACTGACATCGCCTATCGCTGTGGATTCAGCGACAGTAACCACTTTTCGACGCTTTTTCGACGAGAGTTTAACTGGTCACCGCGTGATATTCGCCAgg                                                            <  2:520737/100‑1 (MQ=255)
                                    ttACTGACATCGCCTATCGCTGTGGATTCAGCGACAGTAACCACTTTTCGACGCTTTTTCGACGAGAGTTTAACTGGTCACCGCGTGATATTCGCCTggg                                                           >  2:2275858/1‑100 (MQ=255)
                                     tACTGACATCGCCTATCGCTGTGGATTCAGCGACAGTAACCACTTTTCGACGCTTTTTCGACGAGAGATTAACTGGTCACCGCGTGATATTCGCCAGGGa                                                          >  1:1064522/1‑100 (MQ=255)
                                         gACATCGCCTATCGCTGTGGATTCAGCGACAGTAACCACTTTTCGACGCTTTTTCGACGAGAGTTTAACTGGTCACCGCGTGATATTCGCCAGGGACggg                                                      >  1:1081221/1‑100 (MQ=255)
                                           cATCGCCTATCGCTGTGGATTCAGCGACAGTAACAACTTTTCGACGCTTTTTAGACGAGAGTTTAACTGATCAGGGCGTGATATTCGCAAGGGACg                                                        >  2:634595/1‑96 (MQ=255)
                                            aTCGCCTATCGCTGTGGATTCAGCGACAGTAACCACTTTTCGACGCTTTTTCGACGCGAGTTTAACTGGTCACCGCGTGATATTCGCCAGGGAAGGGAt                                                    <  2:599509/99‑1 (MQ=255)
                                            aTCGCCTATCGCTGTGGATTCAGCGACAGTAACCACTTTTCGACGCTTTTTCGACGAGAGATTAACTGGTCACCGCGTGATATTCGCCAGGGACGGGATg                                                   >  1:932969/1‑100 (MQ=255)
                                             tCGCCTATCGCTGTGGATTCAGCGACAGTAACCACTTTTCGACGCTTTTTCGACGAGAGTTTAACTGGTCACCGCGTGATATTCGCCAGGGACGGGATgg                                                  >  1:1675470/1‑100 (MQ=255)
                                             tCGCCTATCGCTGTGGATTCAGCGACAGTAACCACTTTTCGACGCTTTTTCGACGAGAGTTTAACTGGTCACCGCGTGATATTCGCCAGGGACGGGATgg                                                  <  2:105387/100‑1 (MQ=255)
                                              cGCCTATCGCTGTGGATTCAGCGACAGTAACCACTTTTCGACGCTTTTTCGACGAGAGTTTAACTGGTCACCGCGTGATATTCGCCAGGGACGGGATGGc                                                 <  1:1511543/100‑1 (MQ=255)
                                               gCCTATCGCTGTGGATTCAGCGACAGTAACCACTTTTCGACGCTTTTTCGACGAGAGTTTAACTGGTCACCGCGTGATATTCGCCAGGGACGGGATGGCt                                                >  1:764678/1‑100 (MQ=255)
                                                ccTATCGCTGTGGATTCAGCGAAAGTAACCACTTTTCGACGCTTTTTCGACGAGAGATTAACTGGTAACCGCGTGATATTCGACAGGGACGGGATGGCtt                                               >  2:28352/1‑100 (MQ=255)
                                                  tATCGCTGTGGATTCAGCGACAGTAACCACTTTTCGACGCTTTTTCGACGAGAGTTTAACTGGTCACCGCGTGATATTCGCCAGGGACGGGATGGCTTTc                                             <  2:2320318/100‑1 (MQ=255)
                                                  tATCGCTGTGGATTCAGCGACAATAACCACTTTTCGACGCTTTTTCGACGAGAGTTTAACTGGTCACCGCGTGATATTCGCCAGGGACGGGATGGCTTTc                                             >  2:1320603/1‑100 (MQ=255)
                                                   aTCGCTGTGGATTCAGCGACAGTAACCACTTTTCGACGCTTTTTCGACGAGAGTTTAACTGGTTACCGCGTGATATTCGCCAGGGACGGGATGGCTTTCt                                            <  1:2145431/100‑1 (MQ=255)
                                                   aTCGCTGTGGATTCAGCGACAGTAACCACTTTTCGACGCTTTTTCGACGAGAGTTTAACTGGTCACCGCGTGATATTCGCCAGGGACGGGATGGCTTTCt                                            <  1:312829/100‑1 (MQ=255)
                                                    tctcTGTGGATTCAGCGACAGTAACCACTTTTCGACGCTTTTTCGACGAGAGTTTAACTGGTCACCGCGTGATATTCGCCAGGGACGGGATGGCTTTCTg                                           <  2:215784/97‑1 (MQ=255)
                                                    tCGCTGTGGATTCAGCGACAGTAACCACTTTTCGACGCTTTTTCGACGAGAGTTTAACTGGTCACCGCGTGATATTCGCCAGGGACGGGATGGCTTTCTg                                           <  2:2857015/100‑1 (MQ=255)
                                                    tCGCTGTGGATTCAGCGACAGTAACCACTTTTCGACGCTTTTTCGACGAGAGTTTAACTGGTCACCGCGTGATATTCGCCAGGGACGGGATGGCTTTCTg                                           >  1:290689/1‑100 (MQ=255)
                                                     cGCTGTGGATTCAGCGACAGTAACCACTTTTCGACGCTTTTTCGACGAGAGTTTAACTGGTCACCGCGTGATATTCGCCAGGGACGGGATGGCTTTCTGc                                          <  2:2995523/100‑1 (MQ=255)
                                                       cTGTGGATTCAGCGACAGTAACCACTTTTCGACGCTTTTTCGACGAGAGTTTAACTGGTCACCGCGTGATATTCGCCAGGGACGGGATGGCTTTCTGCaa                                        >  2:2821020/1‑100 (MQ=255)
                                                       cTGTGGATTCAGCGACAGTAACCACTTTTCGACGCTTTTTCGACGAGAGTTTAACTGGTCACCGCGTGATATTCGCCAGGGACGGGATGGCTTTCTGCaa                                        <  1:420468/100‑1 (MQ=255)
                                                       cTGTGGATTCAGCGACAGTAACCACTTTTCGACGCTTTTTCGACGAGAGTTTAACTGGTCACCGCGTGATATTCGCCAGGGACGGGATGGCTTTCTGCaa                                        >  2:893366/1‑100 (MQ=255)
                                                       cTGTGGATTCAGCGACAGTAACCACTCTTCGACGCTTTTTCGACGAGAGTTTAACTGTTCACCGCGTGATATTCACCAGGGACGGAATAGCTTTCTGCaa                                        >  1:1041599/1‑100 (MQ=255)
                                                        tgtgGATTCAGCGACAGTAACCACTTTTCGACGCTTTTTCGACGAGAGTTTAACTGGTCACCGCGTGATATTCGCCAGGGACGGGATGGCTTTCTGCAAt                                       >  2:1725884/1‑100 (MQ=255)
                                                          tgGATTCAGCGACAGTAACCACTTTTCGACGCTTTTTCGACGAGAGTTTAACTGGTCACCGCGTGATATTCGCCAGGGACGGGATGGCTTTCTGCAATaa                                     >  1:2886161/1‑100 (MQ=255)
                                                          tgGATTCAGCGACAGTAACCACTTTTCGACGCTTTTTCGACGAGAGTTTAACTGGTCACCGCGTGATATTCGCCAGGGACGGGATGGCTTTCTGCAATaa                                     <  2:318349/100‑1 (MQ=255)
                                                            gATTCAGCGACAGTAACCACTTTTCGACGCTTTTTCGACGAGAGTTTAACTGGTCACCGCGTGATATTCGCCAGGGACGGGATGGCTTTCTGCAATAAcg                                   >  1:454568/1‑100 (MQ=255)
                                                                cAGCGACAGTAACCACTTTTCGACGCTTTTTCGACGAGAGTTTAACTGGTCACCGCGTGATATTCGCCAGGGACGGGATGGCTTTCTGCAATAACGCGaa                               <  1:1366651/100‑1 (MQ=255)
                                                                cAGCGACAGTAACCACTTTTAGACGATTTTTCGATGAGAGTTTAACTGGTCACCGCGTGATATTCGCCAGGGACGGGATGGCTTTCTGCAATAACGCGaa                               >  2:303365/1‑100 (MQ=255)
                                                                  gggagaGTAACCACTGTTCTACGTTTTTTCGACAAGATGTTAACTGGTCACCGCGTGATATTCGCCAGGGACGGCATGGCTTTCTGCAATAAcgcg                                 <  2:2291629/91‑1 (MQ=255)
                                                                  gctaCAGTAACCACTTTTCGACGCTTTTTCGACGAGAGTTTAACTGGTCACCGCGTGATATTCGCCAGGGACGGGATGGCTTTCTGCAATAACGCGaa                               <  2:2655359/95‑1 (MQ=255)
                                                                  gCGACAGTAACCACTTTTCGACGCTTTTTCGACGAGAGTTTAACTGGTCACCGCGTGATATTCGCCAGGGACGGGATGGCTTTCTGCAATAACGCGAAtc                             >  2:1125955/1‑100 (MQ=255)
                                                                   cGACAGTAACCACTTTTCGACGCTTTTTCGACGAGAGTTTAACTGGTCACCGCGTGATATTCGCCAGGGACTGGATGGCTTTCTGCAATAACGCGAAtct                            <  2:649375/100‑1 (MQ=255)
                                                                     aCAGTAACTACTTTTCGACGCTTTTTCGACGAGAGTTTAACTGGTCACCGCGTGATATTCGCCAGGGACGGGATGGCTTTCTGCAATAACGCGAAtcttc                          <  1:1955337/100‑1 (MQ=255)
                                                                     aCAGTAACCACTTTTCGACGCTTTTTCGACGAGAGTTTAACTGGTCACCGCGTGATATTCGCCAGGGACGGGATGGCTTTCTGCAATAACGCGAAtcttc                          <  1:1955120/100‑1 (MQ=255)
                                                                      cAGTAACCACTTTTCGATGCTTTTTCGATTAGAGTTTTACTGGTCATCGCGTGATATTCGCCAGGGACGGGATGACTTTCTGCAATAACGCGAAtcttct                         <  1:119453/100‑1 (MQ=255)
                                                                      cAGTAACCACTTTTCGACGCTTTTTCGACGAGAGTTTAACTGGTCACCGCGTGATATTCGCCAGGGACGGGATGGCTTTCTGCAATAACGCGAAtcttct                         <  1:1233347/100‑1 (MQ=255)
                                                                       aGTAACCACTTTTCGACGCTTTTTCGACGAGAGTTTAACTTGTCACCGCGTGATATTCGCCAGGGACGGGATGGCTTTCTGCAATAACGCGAATCTtctc                        <  1:575006/100‑1 (MQ=255)
                                                                       aGTAACCACTTTTCGACGCTTTTTCGACGAGAGTTTAACTGGTCACCGCGTGATATTCGCCAGGGACGGGATGGCTTTCTGCAATAACGCGAATCTtctc                        <  1:2809799/100‑1 (MQ=255)
                                                                        gTAACCACTTTTCGACGCTTTTTCGACGAGAGTTTAACTGGTCACCGCGTGATATTCGCCAGGGACGGGATGGCTTTCTGCAATAACGCGAATCTTCTCa                       >  1:1429696/1‑100 (MQ=255)
                                                                         tAACCACTTTTCGACGCTTTTTCGACGAGAGTTTAACTGGTCACCGCGTGATATTCGCCAGGGACGGGATGGCTTTCTGCAATAACGCGAATCTTCTCaa                      >  1:853426/1‑100 (MQ=255)
                                                                          aaCCACTTTTCGACGCTTTTTCGACGAGAGTTTAACTGGTCACCGCGTGATATTCGCCAGGGACGGGATGGCTTTCTGCAATAACGCGAATCTTCTCAAc                     >  2:928363/1‑100 (MQ=255)
                                                                           aCCACTTTTCGACGCTTTTTCGACGAGAGTTTAACTGGTCACCGCGTGATATTCGCCAGGGACGGGATGGCTTTCTGCAATAACGCGAATCTTCTCAACg                    >  1:484179/1‑100 (MQ=255)
                                                                            ccACTTTTCGACGCTTTTTCGACGAGAGTTTCACTGGTCACCGCGTGATATTCGCCAGGGACGGGATGGCTTTCTGCAATAACGCGAATCTTCTCAACGt                   >  1:2415664/1‑100 (MQ=255)
                                                                            ccACTTTTCGACGCTTTTTCGACGAGAGTTTAACTGGTCACCGCGTGATATTCGCCAGGGACGGGATGGCTTTCTGCAATAACGCGAATCTTCTCAACGt                   >  2:3015838/1‑100 (MQ=255)
                                                                             cACTTTTCGACGCTTTTTCGACGAGAGTTTAACTGGTCACCGCGTGATATTCGCCAGGGACGGGATGGCTTTCTGCAATAACGCGAATCTTCTCAACGTa                  >  1:1880897/1‑100 (MQ=255)
                                                                              aCTTTTCGACGCTTTTTCGACGAGAGTTTAACTGGTCACCGCGTGATATTCGCCAGGGACGGGATGGCTTTCTGCAATAACGCGAATCTTCTCAACGTAt                 >  1:2038345/1‑100 (MQ=255)
                                                                              aCTTTTCGACGCTTTTTCGACGAGAGTTTAACTGGTCACCGCCTGATATTCGCCAAGTACGAGATAGCTTTATGCAATAACGCGAATCTTCTCAACGTAt                 >  1:801310/1‑100 (MQ=255)
                                                                               cTTTTCGACGCTTTTTCGACGAGAGTTTAACTGGTCACCGCGTGATATTCGCCAGGGACGGGATGGCTTTCTGCAATAACGCGAATCTTCTCAACGTAtt                >  2:969179/1‑100 (MQ=255)
                                                                               cTTTTCGACGCTTTTTCGACGAGAGTTTAACTGGTCACCGCGTGATATTCGCCAGGGACGGGATGGCTTTCTGCAATAACGCGAATCTTCTCAACGTAtt                >  1:2214460/1‑100 (MQ=255)
                                                                                 tttCGACGCTTTTTCGACGAGAGTTTAACTGGTCACCGCGTGATATTCGCCAGGGACGGGATGGCTTTCTGCAATAACGCGAATCTTCTCAACGTATTTg              <  2:1899246/100‑1 (MQ=255)
                                                                                    cGACGCTTTTTCGACGAGAGTTTAACTGGTCACCGCGTGATATTCGCCAGGGACGGGATGGCTTTCTGCAATAACGCGAATCTTCTCAACGTATTTGTa            >  1:2792963/1‑99 (MQ=255)
                                                                                       cGCTTTTTCGACGAGAGTTTAACTGGTCACCGCGTGATATTCGCCAGGGACGGGATGGCTTTCTGCAATAACGCGAATCTTCTCAACGTATTTGTACGcc        >  1:143801/1‑100 (MQ=255)
                                                                                       cGCTTTTTCGACGAGAGTTTAACTGGTCACCGCGTGATATTCGCCAGGGAAAGGATAGCTTTATGAAATAAAAAGAATATTCTAAACGAAATTGTa            >  1:49232/1‑96 (MQ=255)
                                                                                        gCTTTTTCGACGAGAGTTTAACTGGTCACCGCGTGATATTCGCCAGGGACGGGATGGGTTTCTGCAATAACGCGAATCTTCTCAACGTATTTGTACGCCa       <  1:2473454/100‑1 (MQ=255)
                                                                                        gCTTTTTCGACGAGAGTTTAACTGGTCACCGCGTGATATTCGCCAGGGACGGGATGGCTTTCTGCAATAACGCGAATCTTCTCAACGTATTTGTACGCCa       >  2:2325582/1‑100 (MQ=255)
                                                                                            tttCGACGAGAGTTTAACTGGTCACCGCGTGATATTCGCCAGGGACGGGATGGCTTTCTGCAATAACGCGAATCTTCTCAACGTATTTGTACGCCATAtt   >  1:1688692/1‑100 (MQ=255)
                                                                                             ttCGACGAGAGTTTAACTGGTCACCGCGTGATATTCGCCAGGGACGGGATGGCTTTCTGCAATAACGCGAATCTTCTCAACGTATTTGTACGCCATATTg  <  1:993826/100‑1 (MQ=255)
                                                                                                 |                                                                                               
AAGCCCGACATCTGCTACGCCACAGCGAGGCCAGCGTTACTGACATCGCCTATCGCTGTGGATTCAGCGACAGTAACCACTTTTCGACGCTTTTTCGCCGAGAGTTTAACTGGTCACCGCGTGATATTCGCCAGGGACGGGATGGCTTTCTGCAATAACGCGAATCTTCTCAACGTATTTGTACGCCATATTG  >  NC_000913/4098415‑4098607

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A