Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A12 F56 I0 R1 312 170.3 8276235 93.7% 7754832 99.8

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 4,098,512 C→A 26.1% R259R (CGC→CGA)  rhaS → transcriptional activator of rhaBAD and rhaT

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,098,5120CA26.1% 363.9 / 130.1 196R259R (CGC→CGA) rhaStranscriptional activator of rhaBAD and rhaT
Reads supporting (aligned to +/- strand):  ref base C (79/62);  new base A (31/20);  total (114/82)
Fisher's exact test for biased strand distribution p-value = 6.22e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.72e-01

Only 100 of 198 total aligned reads displayed.

AAAGCCCGACATCTGCTACGCCACAGCGAGGCCAGCGTTACTGACATCGCCTATCGCTGTGGATTCAGCGACAGTAACCACTTTTCGACGCTTTTTCGCCGAGAGTTTAACTGGTCACCGCGTGATATTCGCCAGGGACGGGATGGCTTTCTGCAATAACGCGAATCTTCTCAACGTATTTGTACGCCATATTGCGAA  >  NC_000913/4098414‑4098611
                                                                                                  |                                                                                                   
aaaGCCCGACATCTGCTACGCCACAGCGAGGCCAGCGTTACTGACATCGCCTATCGCTGTGGATTCAGCGACAGTAACCACTTTTCGACGCTTTTTCGcc                                                                                                    <  2:769329/100‑1 (MQ=255)
 aaGCCCGACATCTGCTACGCCACAGCGAGGCCAGCGTTACTGACATCGCCTATCGCTGTGGATTCAGCGACAGTAACCACTTTTCGACGCTTTTTCGCCg                                                                                                   >  1:1344487/1‑100 (MQ=255)
    nccGACATCTGCTACGCCACAGCGAGGCCAGCGTTACTGACATCGCCTATCGCTGTGGATTCAGCGACAGTAACCACTTTTCGACGCTTTTTCGCCgaga                                                                                                >  2:920452/2‑100 (MQ=255)
    cccGACATCTGCTACGCCACAGCGAGGCCAGCGTTACTGACATCGCCTATCGCTGTGGATTCAGCGACAGTAACCACTTTTCGACGCTTTTTCGACgaga                                                                                                >  2:723500/1‑100 (MQ=255)
     ccGACATCTGCTACGCCACAGCGAGGCCAGCGTTACTGACATCGCCTATCGCTGTGGATTCAGCGACAGTAACCACTTTTCGACGCTTTTTCGCCGagag                                                                                               >  1:3750425/1‑100 (MQ=255)
      cGACATCTGCTACGCCACAGCGAGGCCAGCGTTACTGACATCGCCTATCGCTGTGGATTCAGCGACAGTAACCACTTTTCGACGCTTTTTCGCCGAGAGt                                                                                              >  1:3199803/1‑100 (MQ=255)
        aCATCTGCTACGCCACAGCGAGGCCAGCGTTACTGACATCGCCTATCGCTGTGGATTCAGCGACAGTAACCACTTTTCGACGCTTTTTCGCCGAGAGttt                                                                                            <  1:2568191/100‑1 (MQ=255)
           tCTGCTACGCCACAGCGAGGCCAGCGTTACTGACATCGCCTATCGCTGTGGATTCAGCGACAGTAACCACTTTTCGACGCTTTTTCGCCGAGAGTTTAAc                                                                                         <  2:3199784/100‑1 (MQ=255)
           tCTGCTACGCCACAGCGAGGCCAGCGTTACTGACATCGCCTATCGCTGTGGATTCAGCGACAGTAACCACTTTTCGACGCTTTTTCGACGAGAGTTTAAc                                                                                         >  2:1468407/1‑100 (MQ=255)
           tCTGCTACGCCACAGCGAGGACAGCGTTACTGACCTCGCCTATCGCTGTGGATTCAGCGACAGTAGCCACTTTGCGACGCTTTTTCGACGAGAGTTTAAc                                                                                         <  2:1080744/100‑1 (MQ=255)
            cTGCTACGCCACAGCGAGGCCAGCGTTACTGACATCGCCTAACGCTGTGGATTCAGCGACAGTAACCACTTTTCGGCGCTTTTTCGCCGAGAGTTTAACt                                                                                        >  1:1922290/1‑100 (MQ=255)
              gCTACGCCACAGCGAGGCCAGCGTTACTGACATCGCCTATCGCTGTGGATTCAGCGACAGTAACCACTTTTCGACGCTTTTTCGCCGAGAGTTTAACTgg                                                                                      <  1:3243886/100‑1 (MQ=255)
              gCTACGCCACAGCGAGGCCAGCGTTACTGACATCGCCTATCGCTGTGGATTCAGCGACAGTAACCACTTTTCGACGCTTTTTCGCCGAGAGATTAACtag                                                                                      >  2:1417342/1‑98 (MQ=255)
               cTACGCCACAGCGAGGCCAGCGTTACTGACATCGCCTATCGCTGTGGATTCAGCGACAGTAACCACTTTTCGACGCTTTTTCGCCGAGAGTTTAACTGGt                                                                                     >  2:3964873/1‑100 (MQ=255)
                tACGCCACAGCGAGGCCAGCGTTACTGACATCGCCTATCGCTGTGGATTCAGCGACAGTAACCACTTTTCGACGCTTTTTCGCCGAGAGTTTAACTGGTc                                                                                    >  2:2760083/1‑100 (MQ=255)
                   gCCACAGCGAGGCCAGCGTTACTGACATCGCCTATCGCTGTGGATTCAGCGACAGTAACCACTTTTCGACGCTTTTTCGCCGAGAGTTTAACTGGTCAcc                                                                                 >  1:3962489/1‑100 (MQ=255)
                   gCCACAGCGAGGCCAGCGTTACTGACATCGCCTATCGCTGTGGATTCAGCGACAGTAACCACTTTTCGACGCTTTTTCGCCGAGAGTTTAACTGGTCAcc                                                                                 >  2:1348973/1‑100 (MQ=255)
                   gCCACAGCGAGGCCAGCGTTACTGACATCGCCTATCGCTGTGGATTCAGCGACAGTAACCACTTTTCGACGCTTTTTCGCCGAGAGTTTAACTGGTCAcc                                                                                 >  2:52838/1‑100 (MQ=255)
                    ccACAGCGAGGCCAGCGTTACTGACATCGCCTATCGCTGTGGATTCAGCGACAGTAACCACTTTTCGACGCTTTTTCGCCGAGAGTTTAACTGGTCACcg                                                                                >  1:3664209/1‑100 (MQ=255)
                    ccACAGCGAGGCCAGCGTTACTGACATCGCCTATCGCTGTGGATTCAGCGACAGTAACCACTTTTCGACGCTTTTTCGCCGAGAGTTTAACTGGTCACcg                                                                                >  1:1052577/1‑100 (MQ=255)
                    ccACAGCGAGGCCAGCGTTACTGACATCGCCTATCGCTGTGGATTCAGCGACAGTAACCACTTTTCGACGCTTTTTCGACGAGAGTTTAACTGGTCACcg                                                                                >  2:1145946/1‑100 (MQ=255)
                     cacaGCGAGGCCAGCGTTACTGACATCGCCTATCGCTGTGGATTCAGCGACAGTAACCACTTTTCGACGCTTTTTCGCCGAGAGTTTAACTGGTCACcgc                                                                               >  1:1651407/1‑100 (MQ=255)
                       caGCGAGGCCAGCGTTACTGACATCGCCTATCGCTGTGGATTCAGCGACAGTAACCACTTTTCGACGCTTTTTCGCCGAGAGTTTAACTGGTCACCGCGt                                                                             >  2:2915444/1‑100 (MQ=255)
                        aGCGAGGCCAGCGTTACTGACATCGCCTATCGCTGTGGATTCAGCGACAGTAACCACTTTTCGACGCTTTTTCGCCGAGAGTTTAACTGGTCACCGCGTg                                                                            <  2:2315072/100‑1 (MQ=255)
                         gCGAGGCCAGCGTTACTGACATCGCCTATCGCTGTGGATTCAGCGACAGTAACCACTTTTCGACGCTTTTTCGCCGAGAGTTTAACTGGTCAc                                                                                  >  2:3278800/1‑93 (MQ=255)
                         gCGAGGCCAGCGTTACTGACATCGCCTATCGCTGTGGATTCAGCGACAGTAACCACTTTTCGACGC‑TTTTCGCCGAGAGTTTAACTGGTCACCGCGTGat                                                                          >  1:995428/1‑100 (MQ=255)
                           gAGGCCAGCGTTACTGACATCGCCTATCGCTGTGGATTCAGCGACAGTAACCACTTTTCGACGCTTTTTCGCCGAGAGTTTAACTGGTCACCGCGTGata                                                                         <  1:1891893/100‑1 (MQ=255)
                             ggCCAGCGTTACTGACATCGCCTATCGCTGTGGATTCAGCGACAGTAACCACTTTTCGACGCTTTTTCGCCGAGAGTTTAACTTGTCACCGCGTGATAtt                                                                       >  1:1487643/1‑100 (MQ=255)
                             ggCCAGCGTTACTGACATCGCCTATCGCTGTGGATTCAGCGACAGTAACCACTTTTCGACGCTTTTTCGACGAGAGTTTAACTGGTCACCGCGTGATAtt                                                                       >  1:1969145/1‑100 (MQ=255)
                              gCCAGCGTTACTGACATCGCCTATCGCTGTGGATTCAGCGACAGTAACCACTTTTCGACGCTTTTTCGCCGAGGGTTTAACTGGTCACCGCGTGATATTc                                                                      >  1:98273/1‑100 (MQ=255)
                               ccAGCGTTACTGACATCGCCTATCGCTGTGGATTCAGCGACAGTAACCACTTTTCGACGCTTTTTCGCCGAGAGTTTAACTGGTCACCGCGTGATATTCg                                                                     >  2:1941428/1‑100 (MQ=255)
                               ccAGCGTTACTGACATCGCCTATCGCTGTGGATTCAGCGACAGTAACCACTTTTCGACGCTTTTTCGACGAGAGTTTAACTGGTCACCGCGTGATATTCg                                                                     >  2:1630259/1‑100 (MQ=255)
                                cAGCGTTACTGACATCGCCTATCGCTGTGGATTCAGCGACAGTAACCACTTTTCGACGCTTTTTCGCCGAGAGTTTAACTGGTCACCGCGTGATATTCGc                                                                    >  1:2264850/1‑100 (MQ=255)
                                cAGCGTTACTGACATCGCCTATCGCTGTGGATTCAGCGACAGTAACCACTTTTCGACGCTTTTTCGCCGAGAGTTTAACTGGTCACCGCGTGATATTCGc                                                                    >  1:1497376/1‑100 (MQ=255)
                                 aGCGTTACTGACATCGCCTATCGCTGTGGATTCAGCGACAGTAACCACTTTTCGACGCTTTTTCGCCGAGAGTTTAACTGGTCACCGCGTGATATTCGcc                                                                   >  1:4070346/1‑100 (MQ=255)
                                  gCGTTACTGACATCGCCTATCGCTGTGGATTCAGCGACAGTAACCACTTTTCGACGCTTTTTCGCCGAGAGTTTAACTGGTCACCGCGTGATATTCGCCa                                                                  <  2:172898/100‑1 (MQ=255)
                                   cGTTACTGACATCGTCTATCGCTGTGGATTCAGCGACAGTAACCACTTTTCGACGCTTTTTCGCCGAGAGTTTAACTGGTCACCGCGTGATATTCGCCAg                                                                 <  2:1216241/100‑1 (MQ=255)
                                   cGTTACTGACATCGCCTATCGCTGTGGATTCAGCGACAGTAACCACTTTTCGACGCTTTTTCGACGAGAGTTTAACTGGTCACCGCGTGATATTCGCCAg                                                                 <  2:2014805/100‑1 (MQ=255)
                                      tACTGACATCGCCTATCGCTGTGGATTCAGCGACAGTAACCACTTTTCGACGCTTTTTCGACGAGAGTTTAACTGGTCACCGCGTGATATTCGCCAggag                                                              >  1:351905/1‑98 (MQ=255)
                                        cTGACAGCGCCTATCGCTGTGGATTCAGCGACAGTAACCACTTTTCGACGCTTTTTCGCCGAGAGTTTAACTGGTCACCGCGTGATATTCGCCAGGGACg                                                            <  2:2381218/100‑1 (MQ=255)
                                         tGACATCGCCTATCGCTGTGGATTCAGCGACAGTAACCACTTTTCGACGCTTTTTCGCCGAGAGTTTAACTGGTCACCGCGTGATATTCGCCAGGGACgg                                                           <  1:528538/100‑1 (MQ=255)
                                          gACATCGCCTATCGCTGTGGATTCAGCGACAGTAACCACTTTTCGACGCTTTTTCGCCGAGAGTTTAACTGGTCACCGCGTGATATTCGCCAGGGACggg                                                          <  1:3543024/100‑1 (MQ=255)
                                          gACATCGCCTATCGCTGTGGATTCAGCGACAGTAACCACTTTTCGACGCTTTTTCGCCGAGAGTTTAACTGGTCACCGCGTGATATTCGCCAGGGACggg                                                          >  1:2772531/1‑100 (MQ=255)
                                          gACATCGCCTATCGCTGTGGATTCAGCGACAGTAACCACTTTTCGACGCTTTTTCGCCGAGAGTTTAACTGGTCACCGCGTGATATTCGCCAGGGACggg                                                          <  2:3071882/100‑1 (MQ=255)
                                            cATCGCCTATCGCTGTGGATTCAGCGACAGTAACCACTTTTCGACGCTTTTTCGACGAGAGTTTAACTGGTCACCGCGTGATATTCGCCAGGGACGGGAt                                                        <  1:2907455/100‑1 (MQ=255)
                                             agcGCCTATCGCTATGGATTCAGCGACAGTAGCCACTTTTCGACGCTTTTTCGACGAGAGTTTAACTGGTCACCGCGTGATATTCGCCAGGGACGGGATg                                                       >  1:2782099/3‑100 (MQ=255)
                                             aTCTCCTATCGCTGTGGATTCAGCGACAGTAACCACTTTTCGACGCTTTTTCGCCGAGAGTTTAACTGGTCACCGCGTGATATTCGCCAGGGACGGGATg                                                       <  2:656700/100‑1 (MQ=255)
                                             aTCGCCTATCGCTATCGATTCAGCGACACTAACCACTTTTCGACGCTGTTTCGACGAGCGCTTAACTGGTCACCGAGCGTTCTTCGCCAgggcccggctg                                                       >  2:1099098/1‑92 (MQ=255)
                                                 ccTATCGCTGTGGATTCAGCGACAGTAACCACTTTTCGACGCTTTTTCGCCGAGAGTTTAACTGGTCACCGCGTGATATTCGCCAGGGACGGGATGGCtt                                                   <  2:1510595/100‑1 (MQ=255)
                                                  ctgtCGCTGTGGATTCAGCGCCAGTAGCCACTGTTCGGCGCTTTGTCGACTAGAGTTTAACAGGTCACCGCGTGATGTTCGCCAGGGACGGGATGGttt                                                   >  2:1384969/4‑99 (MQ=255)
                                                   tATCGCTGTGGATTCAGCGACAGTAACCACTTTTCGACGCTTTTTCGCCGAGAGTTTAACTGGTCACCGCGTGATATTCGCCAGGGACGGGATGGCTTTc                                                 <  1:2917318/100‑1 (MQ=255)
                                                   tATCGCTGTGGATTCAGCGACAGTAACCACTTTTCGACGCTTTTTCGACGAGAGTTTAACTGGTCACCGCGTGATATTCGCCAGGGACGGGATGGCTTTc                                                 >  2:258831/1‑100 (MQ=255)
                                                     tCGCTGTGGATTCAGCGACAGTATCCACTTTTCGACGCTTTTTCGCCGAGAGTTTAACTGGTCACTGCGTGATATTCGCCAGGGACGGGATGGCTTTCTg                                               <  1:630431/100‑1 (MQ=255)
                                                      cGCTGTGGATTCAGCGACAGTAACCACTTTTCGACGCTTTTTCGCCGAGAGTTTAACTGGTCACCGCGTGATATTCGCCAGGGACGGGATGGCTTTCTGc                                              <  2:3567333/100‑1 (MQ=255)
                                                      cGCTGTGGATTCAGCGACAGTAACCACTTTTCGACGCTTTTTCGCCGAGAGTTTAACTGGTCACCGCGTGATATTCGCCAGGGACGGGATGGCTTTCTGc                                              <  2:1610250/100‑1 (MQ=255)
                                                       gCTGTGGATTCAGCGACAGTAACCACTTTTCGACGCTTTTTCGCCGAGAGTTTAACTGGTCACCGCGTGATATTCGCCAGGGACGGGATGGCTTTCTGCa                                             >  1:2001382/1‑100 (MQ=255)
                                                       gCTGTGGATTCAGCGACAGTAACCACTTTTCGACGCTTTTTCGCCGAGAGTTTAACTGGTCACCGCGTGATATTCGCCAGGGACGGGATGGCTTTCTGCa                                             >  2:3845766/1‑100 (MQ=255)
                                                       gCTGTGGATTCAGCGACAGTAACCACTTTTCGACGCTTTTTCGCCGAGAGTTTAACTGGTCACCGCGTGATATTCGCCAGGGACGGGATGGCTTTCTGCa                                             >  1:2378032/1‑100 (MQ=255)
                                                       gCTGTGGATTCAGCGACAGTAACCACTTTTCGACGCTTTTTCGCCGAGAGGTTAACTGGTCACCGCGTGATATTCGCCAGGGTCGGTATGGCTTTCTGc                                              >  2:2083798/1‑99 (MQ=255)
                                                       gCTGTGGATTCAGCGACAGTAACCACTTTTCGACGCTTTTTCGACGAGAGTTTAACTGGTCACCGCGTGATATTCGCCAGGGACGGGATGGCTTTCTGCa                                             >  2:1751173/1‑100 (MQ=255)
                                                       gCTGTGGATTCAGCGACAGTAACCACTTTTCGACGCTTTTTCGACGAGAGGTTAACTGGTCACCGCGTGATATTCGCCAGGGACGGGATGGCTTTCTGCa                                             >  2:4035308/1‑100 (MQ=255)
                                                         tgtgGATTCAGCGACAGTAACCACTTTTCGACGCTTTTTTTTTTTTTTTTTAACTGGTCACCGCGTCATATTCTCCATCGACGGGATGGCTTTCTGCAAt                                           >  2:583129/1‑100 (MQ=255)
                                                            ggATTCAGCGACAGTAACCACTTTTCGACGCTTTTTCGCCGAGAGTTTAACTGGTCACCGCGTGATATTCGCCAGGGACGGGATGGCTTTCTGCAATAAc                                        <  2:982665/100‑1 (MQ=255)
                                                             gATTCAGCGACAGTAACCACTTTTCGACGCTTTTTCGCCGAGAGTTTAACTGGTCACCGCGTGATATTCGCCAGGGACGGGATGGCTTTCTGCAATAAcg                                       <  1:2129528/100‑1 (MQ=255)
                                                             gATTCAGCGACAGTAACCACTTTTCGACGCTTTTTCGACGAGAGTTTAACTGGTCACCGCGTGATATTCGCCAGGGACGGGATGGCTTTCTGCAATAAcg                                       >  1:3043400/1‑100 (MQ=255)
                                                              aTTCAGCGACAGTAACCACTTTTCGACGCTTTTTCGCCGAGAGTTTAACTGGTCACCGCGTGATATTCGCCAGGGACGGGATGGCTTTCTGCAATAAcgc                                      >  1:269473/1‑100 (MQ=255)
                                                              aTTCAGCGACAGTAACCACTTTTCGACGCTTTTTCGCCGAGAGTTTAACTGGTCACCGCGTGATATTCGCCAGGGACGGGATGGCTTTCTGCAATAAcgc                                      >  1:3405941/1‑100 (MQ=255)
                                                              aTTCAGCGACAGTAACCACTTTTCGACGCTTTTTCGACGAGAGTTTAACTGGTCACCGCGTGATATTCGCCAGGGACGGGATGGCTTTCTGCAATAAcgc                                      >  1:179957/1‑100 (MQ=255)
                                                               ttCAGCGACAGTAACCACTTTTCGACGCTTTTTCGCCGGGAGGTTATCTGGTCACCGCGTGATATTCGCCAGGGACGGGATGGCTTTCTGCAATAAcgcg                                     <  2:513596/100‑1 (MQ=255)
                                                               ttCAGCGACAGTAACCACTTTTCGACGCTTTTTCGCCGAGAGTTTAACTGGTCACCGCGTGATATTCGCCAGGGACGGGATGGCTTTCTGCAATAAcgcg                                     >  1:109866/1‑100 (MQ=255)
                                                               ttCAGCGACAGTAACCACTTTTCGACGCTTTTTCGCCGAGAGTTTAACTGGTCACCGCGTGATATTCGCCAGGGACGGGATGGCTTTCTGCAATAAcgcg                                     >  1:583527/1‑100 (MQ=255)
                                                                 cAGCGACAGTAACCACTTTTCGACGCTTTTTCGACGAGAGTTTAACTGGTCACCGCGTGATATTCGCCAGGGACGGGATGGCTTTCTGCAATAACGCGaa                                   >  2:3311828/1‑100 (MQ=255)
                                                                  agctccAGTAACCACTTTTCGACGCTTTTTCGACGAGAGTTTAACTGGTCACTGCGTGATGTTCGCCAGGGAAGGGATGGCTTTCTGCAATAACGCGaa                                   >  2:2696375/6‑99 (MQ=255)
                                                                  aGCGACAGTAACCACTTTTCGACGCTTTTTTGACGAGAGTTTAAATGGTCACCGCGTGATATTCGCCAGGGACGGGATGGCTTTCTGCAATAACGCGAAt                                  >  2:629896/1‑100 (MQ=255)
                                                                  aGCGACAGTAACCACTTTTCGACGCTTTTTCGCCGAGAGTTTAACTGGTCACCGCGTGATATTCGCCAGGGACGGGATGGCTTTCTGCAATAACGCGAAt                                  >  2:2153275/1‑100 (MQ=255)
                                                                  aGCGACAGTAACCACTTTTCGACGCTTTTTCGCCGAGAGTTTAACTGGTCACCGCGTGATATTCGCCAGGGACGGGATGGCTTTCTGCAATAACGCGAAt                                  >  2:251531/1‑100 (MQ=255)
                                                                  aGCGACAGTAACCACTTTTCGACGCTTTTTCGCCGAGAGGTTAACTGGTCACCGCGTGATATTCGCCAGGGACGGGATGGCTTTCTGCACTAACGCGAAt                                  >  1:1560356/1‑100 (MQ=255)
                                                                   gCGACAGTAACCACTTTTCGACGCTTTTTCGCCGAGAGTTTAACTGGTCACCGCGTGATATTCGCCAGGGACGGGATGGCTTTCTGCAATAACGCGAAtc                                 <  1:1461195/100‑1 (MQ=255)
                                                                   gCGACAGTAACCACTTTTCGACGCTTTTTCGCCGAGAGTTTAACTGGTCACCGCGTGATATTCGCCAGGGACGGGATGGCTTTCTGCAATAACGCGAAtc                                 >  2:1924298/1‑100 (MQ=255)
                                                                      aCAGTAACCACTTTTCGACGCTTTTTCGCCGAGAGTTTAACTGGTCACCGCGTGATATTCGCCAGGGACGGGATGGCTTTCTGCAATAACGCGAAtcttc                              <  1:3439668/100‑1 (MQ=255)
                                                                         gTAACCACTTTTCGACGCTTTTTTTTTTTTTTTTTAACTCGTCACCGCGTGATATTCTCCCACGATGGGATGGTTTTCTACAATTATGCGAAtctt                               >  2:2268188/1‑96 (MQ=255)
                                                                           aaCCACTTTTCGACGCTTTTTCGCCGAGAGTTTAACTGGTCACCGCGTGATATTCGCCAGGGACGGGATGGCTTTCTGCAATAACGCGAATCTTCTCAAc                         <  2:1202164/100‑1 (MQ=255)
                                                                           aaCCACTTTTCGACGCTTTTTCGCCGAGAGTTTAACTGGTCACCGCGTGATATTCGCCAGGGACGGGATGGCTTTCTGCAATAACGCGAATCTTCTCAAc                         <  1:2153288/100‑1 (MQ=255)
                                                                           aaCCACTTTTCGACGCTTTTTCGCCGAGAGTTTAACTGGTCACCGCGTGATATTCGCCAGGGACGGGATGGCTTTCTGCAATAACGCGAATCTTCTCAAc                         >  2:2102793/1‑100 (MQ=255)
                                                                            aCCACTTTTCGACGCTTTTTCGCCGAGAGTTTAACTGGTCACCGCGTGATATTCGCCAGGGACGGGATGGCTTTCTGCAATAACGCGAATCTTCTCAACg                        >  2:466302/1‑100 (MQ=255)
                                                                            aCCACTTTTCGACGCTTTTTCGCCGAGAGTTTAACTGGTCACCGCGTGATATTCGCCAGGGACGGGATGGCTTTCTGCAATAACGCGAATCTTCTCAACg                        >  2:2146044/1‑100 (MQ=255)
                                                                             ccACTTTTCGACGCTTTTTCGCCGAGAGTTTAACTGGTCACCGCGTGATATTCGCCAGGGACGGGATGGCTTTCTGCAATAACGCGAATCTTCTCAACGt                       >  2:1314601/1‑100 (MQ=255)
                                                                             ccACTTTTCGACGCTTTTTCGCCGAGAGTTTAACTGGTCACCGCGTGATATTCGCCAGGGACGGGATGGCTTTCTGCAATAACGCGAATCTTCTCAACGt                       <  2:1560344/100‑1 (MQ=255)
                                                                                   ttCGACGCTTTTTCGCCGAGAGTTTAACTGGTCAGCGCCTGATATTCGCCAGGGACGGGATTGCTTTCTGCAATAACGCGAATCTTCTCAACGTATTTGt                 <  2:852703/100‑1 (MQ=255)
                                                                                     cGACGCTTTTTCGCCGAGAGTTTAACTGGTCACCGCGTGATATTCGCCAGGGACGGGATGGCTTTCTGCAATAACGCGAATCTTCTCAACGTATTTGTAc               >  1:760532/1‑100 (MQ=255)
                                                                                     cGACGCTTTTTCGACGAGAGTTTAACTGGTCACCGCGTGATATTCGCCAGGGACGGGATGGCTTTCTGCAATAACGCGAATCTTCTCAACGTATTTGTAc               >  1:3158972/1‑100 (MQ=255)
                                                                                     cGACGCTTTTTCGACGAGAGTTTAACTGGTCACCGCGTGATATTCGCCAGGGACGGGATGGCTTTCTGCAATAACGCGAATCTTCTCAACGTATTTGTAc               >  1:1711092/1‑100 (MQ=255)
                                                                                      ttccgatcttCGCCGAGAGTTTAACTGGTCACCGCGTGATATTCGCCAGGGACGGGATGGCTTTCTGCAATAACGCGAATCTTCTCAACGTATTTGTACg              <  1:2110139/92‑1 (MQ=255)
                                                                                      gACGCTTTTTCGCCGAGAGGTTAACTGGTCACCGCGTGATATTCGCCAGGGACGGGATGGCGTTCTGCAATAACGCGAATCTTCTCAACGTATTTGTACg              >  2:1918524/1‑100 (MQ=255)
                                                                                      gACGCTTTTTCGACGAGAGTTTAACTGGTCACCGCGTGATATTCGCCAGGGACGGGATGGCTTTCTGCAATAACGCGAATCTTCTCAACGTATTTGTACg              >  1:2466757/1‑100 (MQ=255)
                                                                                       aCGCTTTTTCGACGAGAGTTTAACTGGTCACCGCGTGATATTCGCCAGGGACGGGATGGCTTTCTGCAATAACGCGAATCTTCTCAACGTATTTGTACGc             <  1:723506/100‑1 (MQ=255)
                                                                                        cGCTTTTTCGCCGAGAGTTTAACTGGTCACCGCGTGATATTCGCCAGGGACGGGATGGCTTTCTGCAATAACGCGAATCTTCTCAACGTATTTGTACGcc            >  1:2022738/1‑100 (MQ=255)
                                                                                         gCTTTTTCGCCGAGAGTTTAACTGGTCACCGCGTGATATTCGCCAGGGACGGGATGGCTTTCTGCAATAACGCGAATCTTCTCAACGTATTTGTACGCCa           >  2:362376/1‑100 (MQ=255)
                                                                                               tCGCCGAGAGTTTAACTGGTCACCGCGTGATATTCGCCAGGGACGGGATGGCTTTCTGCAATAACGCGAATCTTCTCAACGTATTTGTACgagatcggaa     >  2:2110126/1‑91 (MQ=255)
                                                                                                  ccGAGAGTTTAACTGGTCACCGCGTGATATTCGCCAGGGACGGGATGGCTTTCTGCAATAACGCGAATCTTCTCAACGTATTTGTACGCCATATTGCGaa  <  1:856417/100‑1 (MQ=255)
                                                                                                  |                                                                                                   
AAAGCCCGACATCTGCTACGCCACAGCGAGGCCAGCGTTACTGACATCGCCTATCGCTGTGGATTCAGCGACAGTAACCACTTTTCGACGCTTTTTCGCCGAGAGTTTAACTGGTCACCGCGTGATATTCGCCAGGGACGGGATGGCTTTCTGCAATAACGCGAATCTTCTCAACGTATTTGTACGCCATATTGCGAA  >  NC_000913/4098414‑4098611

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A