Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A12 F204 I0 R1	247	174.3	8556966	94.2%	8060661	99.8

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_000913	3,016,405	C→T	43.1%	L116F (CTT→TTT)	ygfK →	putative Fe‑S subunit oxidoreductase subunit

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,016,405	0	C	T	43.1%	89.9 / 225.7	188	L116F (CTT→TTT)	ygfK	putative Fe‑S subunit oxidoreductase subunit
Reads supporting (aligned to +/- strand): ref base C (50/57); new base T (46/35); total (96/92)
Fisher's exact test for biased strand distribution p-value = 1.87e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.71e-01

Only 100 of 191 total aligned reads displayed.

AAAACCGTCCAAATTCTTGACCGCCTGGAGCTGGAAAAGCCCTGTATCGATGCCGAAGACGAGTGCTTTAACACCGAATGGTCTACCGAGTTTACCCTGCTTAAAGCCTGGGATGAATACCTCAAAGCCTGGTTTGCCCTGCACCTTCTCGAAGCGATGTTCCAGCCTTCTGATTCCGGTAAATCGTTCATCTTTAATA  >  NC_000913/3016306‑3016504

aaaaCCGTCCAAATTCTTGACCGCCTGGAGCTGGAAAAGCCCTGTATCGATGCCGAAGACGAGTGCTTTAACACCGAATGGTCTACCGAGTTTACCCTGc                                                                                                     >  2:4256856/1‑100 (MQ=255)

 aaaCCGTCCAAATTCTTGACCGCCTGGAGCTGCAAAAGCCCTGTATCGATGCCGAAGACGAGTGCTTTAACACCGAATGGTCTACCGAGTTTACCCTGCt                                                                                                    <  1:1664356/100‑1 (MQ=255)

   aCCGTCCAAATTCTTGACCGCCTGGAGCTGGAAAAGCCCTGTATCGATGCCGAAGACGAGTGCTTTAACACCGAATGGTCTACCGAGTTTACCCTGCTTa                                                                                                  <  2:2217145/100‑1 (MQ=255)

    ccGTCCAAATTCTTGACCGCCTGGAGCTGGAAAAGCCCTGTATCGATGCCGAAGACGAGTGCTTTAACACCGAATGGTCTACCGAGTTTACCCTGTTTa                                                                                                  <  2:3971903/99‑1 (MQ=255)

    ccGTCCAAATTCTTGACCGCCTGGAGCTGGAAAAGCCCTGTATCGATGCCGAAGACGAGTGCTTTAACACCGAATGGTCTACCGAGTTTACCCTGCTTaa                                                                                                 >  2:1406814/1‑100 (MQ=255)

       tCCAAATTCTTGACCGCCTGGAGCTGGAAAAGCCCTGTATCGATGCCGAAGACGAGTGCTTTAACACCGAATGGTCTACCGAGTTTACCCTGTTTAAAGc                                                                                              >  1:2973934/1‑100 (MQ=255)

        ccAAATTCTTGACCGCCTGGAGCTGGAAAAGCCCTGTATCGATGCCGAAGACGAGTGCTTTAACACCGAATGGTCTACCGAGTTTACCCTGTTTAAAGcc                                                                                             >  2:383497/1‑100 (MQ=255)

        ccAAATTCTTGACCGCCTGGAGCTGGAAAAGCCCTGTATCGATGCCGAAGACGAGTGCTTTAACACCGAATGGTCTACCGAGTTTACCCTGCTTAAAGcc                                                                                             <  2:741171/100‑1 (MQ=255)

         cAAATTCTTGACCGCCTGGAGCTGGAAAAGCCCTGTATCGATGCCGAAGACGAGTGCTTTAACACCGAATGGTCTACCGAGTTTACCCTGTTTAAAGCCt                                                                                            >  2:1186366/1‑100 (MQ=255)

         cAAATTCTTGACCGCCTGGAGCTGGAAAAGCCCTGTAGCGATGCCGAAGACGAGTGCTTTGACACCGAATGGTCTACCGAGTTTACCCTGTTTAAAGCCt                                                                                            >  1:2287310/1‑100 (MQ=255)

         cAAATTCTTGACCGCCTGGAGCTGGAAAAGCCCTGTAACGATGCCGAAGACGAGTGCTTTAACACCGAATGGTCTACCGAGTTTACCCTGTTGAAAGCCt                                                                                            >  1:2291484/1‑100 (MQ=255)

               cTTGCCCGCCGGGCGCTGGAAAAGCCCTGTATCGATGCCGAAGACGAGTGCTTTAACACCGAATGGTCTACCGAGTTTACCCTGCTTAAAGCCTGGGATg                                                                                      <  1:2552939/100‑1 (MQ=255)

               cTTGACCGCCTGGAGCTGGAAAAGCCCTGTATCGATGCCGAAGACGAGTGCTTTAACACCGAATGGTCTACCGAGTTTACCCTGTTTAAAGCCTGGGATg                                                                                      <  1:1768446/100‑1 (MQ=255)

                ttGACCGCCTGGAGCTGGAAAAGCCCTGTATCGATGCCGAAGACGAGTGCTTTAACACCGAATGGTCTACCGAGTTTACCCTGCTTACAGCCTGGGATGa                                                                                     <  1:3171552/100‑1 (MQ=255)

                ttGACCGCCTGGAGCTGGAAAAGCCCTGTATCGATGCCGAAGACGAGTGCTTTAACACCGAATGGTCTACCGAGTTTACCCTGCTTAAAGCCTGGGATGa                                                                                     >  1:2232405/1‑100 (MQ=255)

                 tGACCGCCTGGAGCTGGAAAAGCCCTGTATCGATGCCGAAGACGAGTGCTTTAACACCGAATGGTCTACCGAGTTTACCCTGTTTAAAGCCTGGGATGaa                                                                                    >  2:3425342/1‑100 (MQ=255)

                  gACCGCCTGGAGCTGGAAAAGCCCTGTATCGATGCCGAAGACGAGTGCTTTAACACCGAATGGTCTACCGAGTTTACCCTGCTTAAAGCCTGGGATGAAt                                                                                   <  2:2722010/100‑1 (MQ=255)

                    ccGCCTGGAGCTGGAAAAGCCCTGTATCGATGCCGAAGACGAGTGCTTTAACACCGAATGGTCTACCGAGTTTACCCTGTTTAATGCCTGGGATGAATAc                                                                                 >  2:4215555/1‑100 (MQ=255)

                     cGCCTGGAGCTGGAAAAGCCCTGTATCGATGCCGAAGACGAGTGCTTTAACACCGAATGGTCTACCGAGTTTACCCTGTTTAAAGCCTGGGATGAATAcc                                                                                <  1:445835/100‑1 (MQ=255)

                      gCCTGGAGCTGGAAAAGCCCTGTATCGATTCCGAAGACGAGTGCTTTAACACCGAATGGTCTACCGAGTTTACCCTGCTTAAAGCCTGGGATGAATACCt                                                                               <  2:1622587/100‑1 (MQ=255)

                      gCCTGGAGCTGGAAAAGCCCTGTATCGATGCCGAAGACGAGTGCTTTAACACCGAATGGTCTACCGAGTTTACCCTGTTTAAAGCCTGGGATGAATACCt                                                                               <  1:2871019/100‑1 (MQ=255)

                      gCCTGGAGCTGGAAAAGCCCTGTATCGATGCCGAAGACGAGTGCTTTAACACCGAATGGTCTACCGAGTTTACCCTGTTTAAAGCCTGGGATGAATACCt                                                                               >  2:1677294/1‑100 (MQ=255)

                      gCCTGGAGCTGGAAAAGCCCTGTATCGATGCCGAAGACGAGTGCTTTAACACCGAATGGTCTACCGAGTTTACCCTGCTTAAAGCCTGGGATGAATACCt                                                                               >  1:1013305/1‑100 (MQ=255)

                       ccTGGAGCTGGAAAAGCCCTGTATCGATGCCGAAGACGAGTGCTTTAACACCGAATGGTCTACCGAGTTTACCCTGTTTAAAGCCTGGGATGAATACCTc                                                                              >  2:2023428/1‑100 (MQ=255)

                       ccTGGAGCTGGAAAAGCCCTGTATCGATGCCGAAGACGAGTGCTTTAACACCGAATGGTCTACCGAGTTTACCCTGTTTAAAGCCTGGGATGAATACCTc                                                                              <  1:3434874/100‑1 (MQ=255)

                       ccTGGAGCTGGAAAAGCCCTGTATCGATGCCGAAGACGAGTGCTTTAACACCGAATGGTCTACCGAGTTTACCCTGCTTAAAGCCTGGGATGAATACCTc                                                                              >  1:1329181/1‑100 (MQ=255)

                          ggAGCTGGAAAAGCCCTGTATCGATGCCGAAGACGAGTGCTTTAACACCGAATGGTCTACCGAGTTTACCCTGTTTAAAGCCTGGGATGAATACCTCaaa                                                                           >  1:524074/1‑100 (MQ=255)

                           gAGCTGGAAAAGCCCTGTATCGATGCCGAAGACGAGTGCTTTAACACCGAATGGTCTACCGAGTTTACCCTGTTTAAAGCCTGGGATGAATACCTCAAAg                                                                          >  2:859731/1‑100 (MQ=255)

                           gAGCTGGAAAAGCCCTGTATCGATGCCGAAGACGAGTGCTTTAACACCGAATGGTCTACCGAGTTTACCCTGTTTAAAGCCTGGGATGAATACCTCAAAg                                                                          <  1:4215533/100‑1 (MQ=255)

                             gCTGGAAAAGCCCTGTATCGATGCCGAAGACGAGTGCTTTAACACCGAATGGTCTACCGAGTTTACCCTGTTTAAAGCCTGGGATGAATACCTCAAAGcc                                                                        >  2:3319609/1‑100 (MQ=255)

                             gCTGGAAAAGCCCTGTATCGATGCCGAAGACGAGTGCTTTAACACCGAATGGTCTACCGAGTTTACCCTGCTTAAAGCCTGGGATGAATACCTCAAAGcc                                                                        >  2:3242335/1‑100 (MQ=255)

                              cttgAAAAGCCCTGTATCGATGCCGAAGACGAGTGCTTTAACACCGAATGGTCTACCGAGTTTCGCCTGCTTAAAGCATGGGATGAATACTTCAAAGcc                                                                        >  1:1001133/4‑99 (MQ=255)

                              cTGGAAAAGCCCTGTATCGATGCCGAAGACGAGTGCTTTAACACCGAATGGTCTACCGAGTTTACCCTGTTTAAAGCCTGGGATGAATACCTCAAAGCCt                                                                       >  1:694182/1‑100 (MQ=255)

                              cTGGAAAAGCCCTGTATCGATGCCGAAGACGAGTGCTTTAACACCGAATGGTCTACCGAGTTTACCCTGCTTAAAGCCTGGGATGAATACCTCAAAGCCt                                                                       >  1:2205073/1‑100 (MQ=255)

                              cTGGAAAAGCCCTGTATCGATGCCGAAGACGAGTGCTTTAACACCGAATGGTCTACCGAGTTTACCCTGCTTAAAGCCTGGGATGAATACCTCAAAGCCt                                                                       >  1:9081/1‑100 (MQ=255)

                               tGGAAAAGCCCTGTATCGATGCCGAAGACGAGTGCTTTAACACCGAATGGTCTACCGAGTTTACCCTGCTTAAAGCCTGGGATGAATACCTCAAAGCCTg                                                                      <  2:1214704/100‑1 (MQ=255)

                                ggAAAAGCCCTGTATCGATGCCGAAGACGAGTGCTTTAACACCGAATGGTCTACCGAGTTTACCCTGTTTAAAGCCTGGGATGAATACCTCAAAGCCTgg                                                                     >  2:2507006/1‑100 (MQ=255)

                                 gAAAAGCCCTGTATCGATGCCGAAGACGAGTGCTTTAACACCGAATGGTCTACCGAGTTTACCCTGTTTAAAGCCTGGGATGAATACCTCAAAGCCTGGt                                                                    >  1:3421197/1‑100 (MQ=255)

                                      gCCCTGTATCGATGCCGAAGACGAGTGCTTTAACACCGAATGGTCTACCGAGTTTACCCTGCTTAAAGCCTGGGATGAATACCTCAAAGCCTGGTTTGcc                                                               <  2:3735243/100‑1 (MQ=255)

                                       cccTGTATCGATGCCGAAGACGAGTGCTTTAACACCGAATGGTCTACCGAGTTTACCCTGTTTAAAGCCTGGGATGAATACCTCAAAGCCTGGTTTGccc                                                              <  1:3832068/100‑1 (MQ=255)

                                       cccTGTATCGATGCCGAAGACGAGTGCTTTAACACCGAATGGTCTACCGAGTTTACCCTGTTTAAAGCCTGGGATGAATACCTCAAAGCCTGGTTTGccc                                                              <  1:102525/100‑1 (MQ=255)

                                       cccTGTATCGATGCCGAAGACGAGTGCTTTAACACCGAATGGTCTACCGAGTTTACCCTGCTTAAAGCCTGGGATGAATACCTCAAAGCCTGGTTTGccc                                                              <  2:930332/100‑1 (MQ=255)

                                        ccTGTATCGATGCCGAAGACGAGTGCTTTAACACCGAATGGTCTACCGAGTTTACCCTGCTTAAAGCCTGGGATGAATACCTCAAAGCCTGGTTTGCCCt                                                             >  1:3351863/1‑100 (MQ=255)

                                         cTGTATCGATGCCGAAGACGAGTGCTTTAACACCGAATGGTCTACCGAGTTTACCCTGTTTAAAGCCTGGGACGAATACCTCAAAGCCTGGTTTGCCCTg                                                            >  2:3037326/1‑100 (MQ=255)

                                           gTATCGATGCCGAAGACGAGTGCTTTAACACCGAATGGTCTACCGAGTTTACCCTGTTTAAAGCCTGGGATGAATACCTCAAAGCCTTGTTTGCCCTGCa                                                          <  2:1979180/100‑1 (MQ=255)

                                                gATGCCGAAGACGAGTGCTTTAACACCGAATGGTCTACCGAGTTTACCCTGCTTAAAGCCTGGGATGAATACCTCAAAGCCTGGTTTGCCCTGCACCTtc                                                     >  2:2191470/1‑100 (MQ=255)

                                                   gCCGAAGACGAGTGCTTTAACACCGAATGGTCTACCGAGTTTACCCTGTTTAAAGCCTGGGATGAATACCTCAAAGCCTGGTTTGCCCTGCACCTTCTCg                                                  >  2:4137221/1‑100 (MQ=255)

                                                   gCCGAAGACGAGTGCTTTAACACCGAATGGTCTACCGAGTTTACCCTGCTTAAAGCCTGGGATGAATACCTCAAAGCCTGGTTTGCCCTGCACCTTCTCg                                                  >  1:61726/1‑100 (MQ=255)

                                                   gCCGAAGACGAGTGCTTTAACACCGAATGGTCTACCGAGTTTACCCTGCTTAAAGCCTGGGATGAATACCTCAAAGCCTGGTTTGCCCTGCACCTTCTCg                                                  <  2:2539738/100‑1 (MQ=255)

                                                    ccGAAGACGAGTGCTTTAACACCGAATGGTCTACCGAGTTTACCCTGCTTAAAGCCTGGGATGAATACCTCAAAGCCTGGTTTGCCCTGCACCTTCTCGa                                                 >  1:735482/1‑100 (MQ=255)

                                                     cGAAGACGAGTGCTTTAACACCGAATGGTCTACCGAGTTTACCCTGCTTAAAGCCTGGGATGAATACCTCAAAGCCTGGTTTGCCCTGCACCTTCTCGaa                                                >  2:4030782/1‑100 (MQ=255)

                                                      gAAGACGAGTGCTTTAACACCGAATGGTCTACCGAGTTTACCCTGTTTAAAGCCTGGGATGAATACCTCAAAGCCTGGTTTGCCCTGCACCTTCTCGAAg                                               <  1:265057/100‑1 (MQ=255)

                                                      gAAGACGAGTGCTTTAACACCGAATGGTCTACCGAGTTTACCCTGCTTAAAGCCTGGGATGAATACCTCAAAGCCTGGTTTGCCCTGCACCTTCTCGAAg                                               <  1:1597024/100‑1 (MQ=255)

                                                       tctaCGAGTGCTTTAACACCGAATGGTCTACCGAGTTTACCCTGTTTAAAGCCTGGGATGAATACCTCAAAGCCTGGTTTGCCCTGCACCTTCTCGAAGc                                              <  2:653437/97‑1 (MQ=255)

                                                       aaGACGAGTGCTTTAACACCGAATGGTCTACCGAGTTTACCCTGTTTAAAGCCTGGGATGAATACCTCAAAGCCTGGTTTGCCCTGCACCTTCTCGAAGc                                              <  1:2130136/100‑1 (MQ=255)

                                                       aaGACGAGTGCTTTAACACCGAATGGTCTACCGAGTTTACCCTGTTTAAAGCCTGGGATGAATACCTCAAAGCCTGGTTTGCCCTGCACCTTCTCGAAGc                                              <  1:2393668/100‑1 (MQ=255)

                                                       aaGACGAGTGCTTTAACACCGAATGGTCTACCGAGTTTACCCTGCTTAAAGCCTGGGATGAATACCTCAAAGCCTGGTTTGCCCTGCACCTTCTCGAAGc                                              <  2:1777027/100‑1 (MQ=255)

                                                           cGAGTGCTTTAACACCGAATGGTCTACCGAGTTTACCCTGTTTAAAGCCTGGGATGAATACCTCAAAGCCTGGTTTGCCCTGCACCTTCTCGAAGCGATg                                          <  2:515458/100‑1 (MQ=255)

                                                           cGAGTGCTTTAACACCGAATGGTCTACCGAGTTTACCCTGTTTAAAGCCTGGGATGAATACCTCAAAGCCTGGTTTGCCCTGCACCTTCTCGAAGCGATg                                          >  1:417938/1‑100 (MQ=255)

                                                           cGAGTGCTTTAACACCGAATGGTCTACCGAGTTTACCCTGTTTAAAGCCTGGGATGAATACCTCAAAGCCTGGTTTGCCCTGCACCTTCTCGAAGCGATg                                          >  1:417153/1‑100 (MQ=255)

                                                           cGAGTGCTTTAACACCGAATGGTCTACCGAGTTTACCCTGCTTAAAGCCTGGGATGAATACCTCAAAGCCTGGTTTGCCCTGCACCTTCTCGAAGCGATg                                          >  2:3880197/1‑100 (MQ=255)

                                                           cGAGTGCTTTAACACCGAATGGTCTACCGAGTTTACCCTGCTTAAAGCCTGGGATGAATACCTCAAAGCCTGGTTTGCCCTGCACCTTCTCGAAGCGATg                                          >  1:1913886/1‑100 (MQ=255)

                                                            gAGTGCTTTAACACCGAATGGTCTACCGAGTTTACCCTGTTTAAAGCCTGGGATGAATACCTCAAAGCCTGGTTTGCCCTGCACCTTCTCGAAGCGATGt                                         >  2:2350833/1‑100 (MQ=255)

                                                              gTGCTTTAACACCGAATGGTCTACCGAGTTTACCCTGCTTAAAGCCTGGGATGAATACCTCAAAGCCTGGTTTGCCCTGCACCTTCTCGAAGCGATGTTc                                       <  2:3564675/100‑1 (MQ=255)

                                                               tGCTTTAACACCGAATGGTCTACCGAGTTTACCCTGTTTAACGCCTGGGATGAATACCTCAAAGCCTGGTTTGCCCTGCACCTTCTCGAAGCGATGTTc                                       <  1:1247652/99‑1 (MQ=255)

                                                                gCTTTAACACCGAATGGTCTACCGAGTTTACCCTGTTTAAAGCCTGGGATGAATACCTCAAAGCCTGGTTTGCCCTGCACCTTCTCGAAGCGATGTTCCa                                     >  2:3704401/1‑100 (MQ=255)

                                                                gCTTTAACACCGAATGGTCTACCGAGTTTACCCTGCTTAAAGCCTGGGATGAATACCTCAAAGCCTGGTTTGCCCTGCACCTTCTCGAAGCGATGTTCCa                                     >  2:1310485/1‑100 (MQ=255)

                                                                 cTTTAACACCGAATGGTCTACCGAGTTTACCCTGTTTAAAGCCTGGGATGAATACCTCAAAGCCTGGTTTGCCCTGCACCTTCTCGAAGCGATGTTCCAg                                    >  1:1502778/1‑100 (MQ=255)

                                                                 cTTTAACACCGAATGGTCTACCGAGTTTACCCTGCTTAAAGCCTGGGATGAATACCTCAAAGCCTGGTTTGCCCTGCACCTTCTCGGAGCGATGTTCCAg                                    <  1:903674/100‑1 (MQ=255)

                                                                 cTTTAACACCGAATGGTCTACCGAGTTTACCCTGCTTAAAGCCTGGGATGAATACCTCAAAGCCTGGTTTGCCCTGCACCTTCTCGAAGCGATGTTCCAg                                    >  2:475035/1‑100 (MQ=255)

                                                                  tttAACACCGAATGGTCTACCGAGTTTACCCTGTTTAAAGCCTGGGATGAATACCTCAAAGCCTGGTTTGCCCTGCACCTTCTCGAAGCGATGTTCCAGc                                   <  1:2070441/100‑1 (MQ=255)

                                                                  tttAACACCGAATGGTCTACCGAGTTTACCCTGCTTAAAGCCTGGGATGAATACCTCAAAGCCTGGTTTGCCCTGCACCTTCTCGAAGCGATGTTCCAGc                                   <  1:4234156/100‑1 (MQ=255)

                                                                      acacCGAATGGTCTACCGAGTTTACCCTGCTTAAAGCCTGGGATGAATACCTCAAAGCCTGGTTTGCCCTGCACCTTCTCGAAGCGATGTTCCAGCCTTc                               >  2:771267/1‑100 (MQ=255)

                                                                       cacCGAATGGTCTACCGAGTTTACCCTGCTTAAAGCCTGGGATGAATACCTCAAAGCCTGGTTTGCCCTGCACCTTCTCGAAGCGATGTTCCAGCCTTCt                              >  2:3151501/1‑100 (MQ=255)

                                                                       cacCGAATGGTCTACCGAGTTTACCCTGCTTAAAGCCTGGGATGAATACCTCAAAGCCTGGTTTGCCCTGCACCTTCTCGAAGCGATGTTCCAGCCTTCt                              <  2:4101321/100‑1 (MQ=255)

                                                                       cacCGAATGGTCTACCGAGTTTACCCTGCTTAAAGCCTGGGATGAATACCTCAAAGCCTGGTTTGCCCTGCACCTTCTCGAAGCGATGTTCCAGCCTTCt                              >  2:807037/1‑100 (MQ=255)

                                                                        acCGAATGGTCTACCGAGTTTACCCTGCTTAAAGCCTGGGATGAATACCTCAAAGCCTGGTTTGCCCTGCACCTTCTCGAAGCGATGTTCCAGCCTTCTg                             >  1:2433655/1‑100 (MQ=255)

                                                                        acCGAATGGTCTACCGAGTTTACCCTGCTTAAAGCCTGGGATGAATACCTCAAAGCCTGGTTTGCCCTGCACCTTCTCGAAGCGATGTTCCAGCCTTCTg                             <  1:376272/100‑1 (MQ=255)

                                                                        acCGAATGGTCTACCGAGTTTACCCTGCTTAAAGCCTGGGATGAATACCTCAAAGCCTGGTTTGCCCTGCACCTTCTCGAAGCGATGTTCCAGCCTTCTg                             <  2:2622523/100‑1 (MQ=255)

                                                                             aTGGTCTACCGAGTTTACCCTGTTTAAAGCCTGGGATGAATACCTCAAAGCCTGGTTTGCCCTGCACCTTCTCGAAGCGATGTTCCAGCCTTCTGATTcc                        <  2:3689278/100‑1 (MQ=255)

                                                                              tGGTCTACCGAGTTTACCCTGCTTAAAGCCTGGGATGAATACCTCAAAGCCTGGTTTGCCCTGCACCTTCTCGAAGCGATGTTCCAGCCTTCTGATTCCg                       <  2:1759872/100‑1 (MQ=255)

                                                                               ggTCTACCGAGTTTACCCTGCTTAAAGCCTGGGATGAATACCTCAAAGCCTGGTTTGCCCTGCACCTTCTCGAAGCGATGTTCCAGCCTTCTGATTCCgg                      >  2:2005762/1‑100 (MQ=255)

                                                                               ggTCTACCGAGTTTACCCTGCTTAAAGCCTGGGATGAATACCTCAAAGCCTGGTTTGCCCTGCACCTTCTCGAAGCGATGTTCCAGCCTTCTGATTCCgg                      <  1:4145071/100‑1 (MQ=255)

                                                                               ggTCTACCGAGTCTACCCTGCTTAAAGCCTGGGATGAATACCTCAAAGCCTGGTTTGCCCTGCACCTTCTCGAAGCGATGTTCCAGCCTTCTGATTCCg                       <  2:3849790/99‑1 (MQ=255)

                                                                                   tACCGAGTTTACCCTGTTTAAAGCCTGGGATGAATACCTCAAAGCCTGGTTTGCCCTGCACCTTCTCGAAGCGATGTTCCAGCCTTCTGATTCCGGTaaa                  <  2:3750913/100‑1 (MQ=255)

                                                                                         gTTTACCCTGCTTAAAGCCTGGGATGAATACCTCAAAGCCTGGTTTGCCCTGCACCTTCTCGAAGCGATGTTCCAGCCTTCTGATTCCGGTAAATCGTTc            >  1:1974839/1‑100 (MQ=255)

                                                                                         gTTTACCCTGCTTAAAGCCTGGGATGAATACCTCAAAGCCTGGTTTGCCCTGCACCTTCTCGAAGCGATGTTCCAGCCTTCTGATTCCGGTAAATCGTTc            <  2:4095217/100‑1 (MQ=255)

                                                                                         gTTTACCCTGCTTAAAGCCTGGGATGAATACCTCAAAGCCTGGTTTGCCCTGCACCTTCTCGAAGCGATGTTCCAGCCTTCTGATTCCGGTAAATCGTTc            <  2:3521015/100‑1 (MQ=255)

                                                                                         gTTTACCCTGCTTAAAGCCTGGGATGAATACCTCAAAGCCTGGTTTGCCCTGCACCTTCTCGAAGCGATGTTCCAGCCTTCTGATTCCGGTAAATCGTTc            <  2:1031384/100‑1 (MQ=255)

                                                                                         gTTTACCCTGCTTAAAGCCTGGGATGAATACCTCAAAGCCTGGTTTGCCCTGCACCTTCTCGAAGCGATGTTCCAGCCTTCTGATTCCGGTAAATCGTTc            <  2:1058335/100‑1 (MQ=255)

                                                                                          tttACCCTGCTTAAAGCCTGGGATGAATACCTCAAAGCCTGGTTTGCCCTGCACCTTCTCGAAGCGATGTTCCAGCCTTCTGATTCCGGTAAATCGTTCa           <  2:3767356/100‑1 (MQ=255)

                                                                                             aCCCTGTTTAAAGCCTGGGATGAATACCTCAAAGCCTAGTTTGCCCTGCACCTTCTCGAAGCGATGTTCCAGCCTTCTGATTCCGGTAAATCGTTCATCt        >  1:2271436/1‑100 (MQ=255)

                                                                                             aCCCTGCTTAAAGCCTGGGATGAATACCTCAAAGCCTGGTTTGCCCTGCACCTTCTCGAAGCGATGTTCCAGCCTTCTGATTCCGGTAAATCGTTCATCt        >  2:170612/1‑100 (MQ=255)

                                                                                              cccTGTTTAAAGCCTGGGATGAATACCTCAAAGCCTGGTTTGCCCTGCACCTTCTCGCAGCGATGTTCCAGCCTTCTGATTCCGGCAAATCGTTCATCtt       >  1:4112878/1‑100 (MQ=255)

                                                                                              cccTGCTTAAAGCCTGGGATGAATACCTCAAAGCCTGGTTTGCCCTGCACCTTCTCGAAGCGATGTTCCAGCCTTCTGATTCCGGTAAATCGTTCATCtt       <  2:3046155/100‑1 (MQ=255)

                                                                                                cTGTTTAAAGCCTGGGATGAATACCTCAAAGCCTGGTTTGCCCTGCACCTTCTCGAAGCGATGTTCCAGCCTTCTGATTCCGGTAAATCGTTCATCTTTa     >  2:4238504/1‑100 (MQ=255)

                                                                                                cTGTTTAAAGCCTGGGATGAATACCTCAAAGCCTGGTTTGCCCTGCACCTTCTCGAAGCGATGTTCCAGCCTTCTGATTCCGGTAAATCGTTCATCTTTa     <  2:354153/100‑1 (MQ=255)

                                                                                                cTGCTTAAAGCCTGGGATGAATACCTCAAAGCCTGGTTTGCCCTGCACCTTCTCGAAGCGATGTTCCAGCCTTCTGATTCCGGTAAATCGTTCATCTTTa     >  1:2723004/1‑100 (MQ=255)

                                                                                                   cTTAAAGCCTGGGATGAATACCTCAAAGCCTGGTTTGCCCTGCACCTTCTCGAAGCGATGTTCCAGCCTTCTGATTCCGGTAAATCGTTCATCTTTAata  >  2:692148/1‑100 (MQ=255)

                                                                                                   cTTAAAGCCTGGGATGAATACCTCAAAGCCTGGTTTGCCCTGCACCTTCTCGAAGCGATGTTCCAGCCTTCTGATTCCGGTAAATCGTTCATCTTTAata  <  2:2662481/100‑1 (MQ=255)

AAAACCGTCCAAATTCTTGACCGCCTGGAGCTGGAAAAGCCCTGTATCGATGCCGAAGACGAGTGCTTTAACACCGAATGGTCTACCGAGTTTACCCTGCTTAAAGCCTGGGATGAATACCTCAAAGCCTGGTTTGCCCTGCACCTTCTCGAAGCGATGTTCCAGCCTTCTGATTCCGGTAAATCGTTCATCTTTAATA  >  NC_000913/3016306‑3016504

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 22 ≤` `ATCG/ATCG` `< 32 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A