Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A12 F133 I0 R1	200	171.4	8351354	94.3%	7875326	99.8

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_000913	3,016,405	C→T	77.8%	L116F (CTT→TTT)	ygfK →	putative Fe‑S subunit oxidoreductase subunit

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,016,405	0	C	T	77.8%	337.1 / 99.2	180	L116F (CTT→TTT)	ygfK	putative Fe‑S subunit oxidoreductase subunit
Reads supporting (aligned to +/- strand): ref base C (21/19); new base T (64/76); total (85/95)
Fisher's exact test for biased strand distribution p-value = 4.77e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.16e-01

Only 100 of 181 total aligned reads displayed.

AACCGTCCAAATTCTTGACCGCCTGGAGCTGGAAAAGCCCTGTATCGATGCCGAAGACGAGTGCTTTAACACCGAATGGTCTACCGAGTTTACCCTGCTTAAAGCCTGGGATGAATACCTCAAAGCCTGGTTTGCCCTGCACCTTCTCGAAGCGATGTTCCAGCCTTCTGATTCCGGTAAATCGTTCATCTTTAATA  >  NC_000913/3016308‑3016504

aaCCGTCCAAATTCTTGACCGCCTGGAGCTGGAAAAGCCCTGTATCGATGCCGAAGACGAGTGCTTTAACACCGAATGGTCTACCGAGTTTACCCTGCt                                                                                                    <  1:3068182/99‑1 (MQ=255)

 aCCGTCCAAATTCTTGACCGCCTGGAGCTGGAAAAGCCCTGTATCGATGCCGAAGACGAGTGCTTTAACACCGAATGGTCTACCGAGTTTACCCTGCTTa                                                                                                  <  2:2335066/100‑1 (MQ=255)

    gTCCAAATTCTTGACCGCCTGGAGCTGGAAAAGCCCTGTATCGATGCCGAAGACGAGTGCTTTAACACCGAATGGTCTACCGAGTTTACCCTGTTTAAAg                                                                                               >  1:1007898/1‑100 (MQ=255)

    gTCCAAATTCTTGACCGCCTGGAGCTGGAAAAGCCCTGTATCGATGCCGAAGACGAGTGCTTTAACACCGAATGGTCTACCGAGTTTACCCTGTTTAAAg                                                                                               >  1:337037/1‑100 (MQ=255)

    gTCCAAATTCTTGACCGCCTGGAGCTGGAAAAGCCCTGTATCGATGCCGAAGACGAGTGCTTTAACACCGAATGGTCTACCGAGTTTACCCTGTTTAAAg                                                                                               <  2:1716805/100‑1 (MQ=255)

       cAAATTCTTGACCGCCTGGAGCTGGAAAAGCCCTGTATCGATGCCGAAGACGAGTGCTTTAACACCGAATGGTCTACCGAGTTTACCCTGTTTAAAGCCt                                                                                            >  1:3701050/1‑100 (MQ=255)

         aaTTCTTGACCGCCTGGAGCTGGAAAAGCCCTGTATCGATGCCGAAGACGAGTGCTTTAACACCGAATGGTCTACCGAGTTTACCCTGTTTAAAGCCTgg                                                                                          <  1:3620505/100‑1 (MQ=255)

         aaTTCTTGACCGCCTGGAGCTGGAAAAGCCCTGTATCGATGCCGAAGACGAGTGCTTTAACACCGAATGGTCTACCGAGTTTACCCTGTTTAAAGCCTgg                                                                                          <  1:787072/100‑1 (MQ=255)

         aaTTCTTGACCGCCTGGAGCTGGAAAAGCCCTGTATCGATGCCGAAGACGAGTGCTTCAACACCGAATGGTCTACCGAGTTTACCCTGTTTAAAGCCttg                                                                                          <  1:2811444/100‑3 (MQ=255)

          aTTCTTGACCGCCTGGAGCTGGAAAAGCCCTGTATCGATGCCGAAGACGAGTGCTTTAACACCGAATGGTCTACCGAGTTTACCCTGTTTAAAGCCTggg                                                                                         <  1:2472921/100‑1 (MQ=255)

           ttCTTGACCGCCTGGAGCTTGAAAAGCCCTGTATCGATGCCGAAGACGTGTGCTTTAACACCGAATGGTCTACCGAGTTTACCCTGTTTAAAGCCTggg                                                                                         <  2:3940725/99‑1 (MQ=255)

            tCTTGACCGCCTGGAGCTGGAAAAGCCCTGTATCGATGCCGAAGACGAGTGCTTTAACACCGAATGGTCTACCGAGTTTACCCTGTTTAAAGCCTGGGAt                                                                                       <  2:3637455/100‑1 (MQ=255)

             cTTGACCGCCTGGAGCTGGAAAAGCCCTGTATCGATGCCGAAGACGAGTGCTTTAACACCGAATGGTCTACCGAGTTTACCCTGTTTAAAGCCTGGGATg                                                                                      <  2:1115189/100‑1 (MQ=255)

             cTTGACCGCCTGGAGCTGGAAAAGCCCTGTATCGATGCCGAAGACGAGTGCTTTAACACCGAATGGTCTACCGAGTTTACCCTGTTTAAAGCCTGGGATg                                                                                      >  2:3440033/1‑100 (MQ=255)

               tGACCGCCTGGAGCTGGAAAAGCCCTGTATCGATGCCGAAGACGAGTGCCTTAACACCGAATGGTCTACCGAGTTTACCCTGTTTAAAGCCTGGGATGa                                                                                     <  1:3612606/99‑1 (MQ=255)

                gACCGCCTGGAGCTGGAAAAGCCCTGTATCGATGCCGAAGACGAGTGCTTTAACACCGAATGGTCTACCGAGTTTACCCTGTTTAAAGCCTGGGATGAAt                                                                                   >  2:639233/1‑100 (MQ=255)

                  ccGCCTGGAGCTGGAAAAGCCCTGTATCGATGCCGAAGACGAGTGCTTTAACACCGAATGGTCTACCGAGTTTACCCTGTTTAAAGCCTGGGATGAATAc                                                                                 >  1:1436577/1‑100 (MQ=255)

                       tGGAGCTGGAAAAGCCCTGTATCGATGCCGAAGACGAGTGCTTTAACACCGAATGGTCTACCGAGTTTACCCTGCTTAAAGCCTGGGATGAATACCTCaa                                                                            >  2:3571318/1‑100 (MQ=255)

                         gAGCTGGAAAAGCCCTGTATCGATGCCGAAGACGAGTGCTTTAACACCGAATGGTCTACCGAGTTTACCCTGCTTAAAGTCTGGGATGAATACCTCAAAg                                                                          <  2:2538936/100‑1 (MQ=255)

                          aGCTGGAAAAGCCCTGTATCGATGCCGAAGACGAGTGCTTTAACACCGAATGGTCTACCGAGTTTACCCTGTTTAAAGCCTGGGATGAATACCTCAAAGc                                                                         >  2:871169/1‑100 (MQ=255)

                           gCTGGAAAAGCCCTGTATCGATGCCGAAGACGAGTGCTTTAACACCGAATGGTCTACCGAGTTTACCCTGCTTAAAGCCTGGGATGAATACCTCAAAGcc                                                                        >  2:4078550/1‑100 (MQ=255)

                            cTGGAAAAGCCCTGTATCGATGCCGAAGACGAGTGCTTTAACACCGAATGGTCTACCGAGTTTACCCTGTTTAAAGCCTGGGATGAATACCTCAAAGCCt                                                                       <  1:1143313/100‑1 (MQ=255)

                            cTGGAAAAGCCCTGTATCGATGCCGAAGACGAGTGCTTTAACACCGAATGGTCTACCGAGTTTACCCTGTTTAAAGCCTGGGATGAATACCTCAAAGCCt                                                                       >  1:659139/1‑100 (MQ=255)

                              ggAAAAGCCCTGTATCGATGCCGAAGACGAGTGCTTTAACACCGAATGGTCTACCGAGTTTACCCTGTTTAAAGCCTGGGATGAATACCTCAAAGCCTgg                                                                     >  1:922434/1‑100 (MQ=255)

                                   aGCCCTGTATCGATGCCGAAGACGAGTGCTTTAACACCGAATGGTCTACCGAGTTTACCCTGTTTAAAGCCTGGGATGAATACCTCAAAGCCTGGTTTGc                                                                <  1:1170005/100‑1 (MQ=255)

                                    gCCCTGTATCGATGCCGAAGACGAGTGCTTTAACACCGAATGGTCTACCGAGTTTACCCTGTTTAAAGCCTGGGATGAATACCTCAAAGCCTGGTTTGcc                                                               >  2:2469785/1‑100 (MQ=255)

                                    gCCCTGTATCGATGCCGAAGACGAGTGCTTTAACACCGAATGGTCTACCGAGTTTACCCTGTTTAAAGCCTGGGATGAATACCTCAAAGCCTGGTTTGcc                                                               >  1:2079839/1‑100 (MQ=255)

                                    gCCCTGTATCGATGCCGAAGACGAGTGCTTTAACACCGAATGGTCTACCGAGTTTACCCTGTTTAAAGCCTGGGATGAATACCTCAAAGCCTGGTTTGcc                                                               >  2:1323281/1‑100 (MQ=255)

                                     cccTGTATCGATGCCGAAGACGAGTGCTTTAACACCGAATGGTCTACCGAGTTTACCCTGTTTAAAGCCTGGGATGAATACCTCAAAGCCTGGTTTGccc                                                              >  1:355212/1‑100 (MQ=255)

                                      ccTGTATCGATGCCGAAGACGAGTGCTTTAACACCGAATGGTCTACCGAGTTTACCCTGTTTAAAGCCTGGGATGAATACCTCAAAGCCTGGTTTGCCCt                                                             >  2:1093205/1‑100 (MQ=255)

                                      ccTGTATCGATGCCGAAGACGAGTGCTTTAACACCGAATGGTCTACCGAGTTTACCCTGTTTAAAGCCTGGGATGAATACCTCAAAGCCTGGTTTGCCCt                                                             >  1:1768104/1‑100 (MQ=255)

                                        tGTATCGATGCCGAAGACGAGTGCTTTAACACCGAATGGTCTACCGAGTTTACCCTGTTTAAAGCCTGGGATGAATACCTCAAAGCCTGGTTTGCCCTGc                                                           <  2:3511724/100‑1 (MQ=255)

                                        tGTATCGATGCCGAAGACGAGTGCTTTAACACCGAATGGTCTACCGAGTTTACCCTGTTTAAAGCCTGCGATGAATACCTCAAAGCCTGGTTTGCCCTGc                                                           >  2:2071849/1‑100 (MQ=255)

                                         gTATCGATGCCGAAGACGAGTGCTTTAACACCGAATGGTCTACCGAGTTTACCCTGCTTAAAGCCTGGGATGAATACCTCAAAGCCTGGTTTGCCCTGCa                                                          <  1:117475/100‑1 (MQ=255)

                                          tATCGATGCCGAAGACGAGTGCTTTAACACCGAATGGTCTACCGAGTTTACCCTGTTTAAAGGCTGGGATGAATACCTCAAAGCCTGGTTTGCCCTGCAc                                                         <  1:4143911/100‑1 (MQ=255)

                                          tATCGATGCCGAAGACGAGTGCTTTAACACCGAATGGTCTACCGAGTTTACCCTGTTTAAAGCCTGGGATGAATACCTCAAAGCCTGGTTTGCCCTGCAc                                                         <  2:3532506/100‑1 (MQ=255)

                                            tCGATGCCGAAGACGAGTGCTTTAACACCGAATGGTCTACCGAGTTTACCCTGTTTAAAGCCTGGGATGAATACCTCAAAGCCTGGTTTGCCCTGCACCt                                                       <  2:3776617/100‑1 (MQ=255)

                                             cGATGCCGAAGACGAGTGCTTTAACACCGAATGGTCTACCGAGTTTACCCTGTTTAAAGCCTGGGATGAATACCTCAAAGCCTGGTTTGCCCTGCACCtt                                                      >  1:2874310/1‑100 (MQ=255)

                                             cGATGCCGAAGACGAGTGCTTTAACACCGAATGGTCTACCGAGTTTACCCTGTTTAAAGCCTGGGATGAATACCTCAAAGCCTGGTTTGCCCTGCACCtt                                                      >  2:285728/1‑100 (MQ=255)

                                             cGATGCCGAAGACGAGTGCTTTAACACCGAATGGTCTACCGAGTTTACCCTGCTTAAAGCCTGGGATGAATACCTCAAAGCCTGGTTTGCCCTGCACCtt                                                      >  1:1247607/1‑100 (MQ=255)

                                                tGCCGAAGACGAGTGCTTTAACACCGAATGGTCTACCGAGTTTACCCTGTTTAAAGCCTGGGATGAATACCTCAAAGCCTGGTTTGCCCTGCACCTtctc                                                   <  1:2706003/100‑1 (MQ=255)

                                                 gCCGAAGACGAGTGCTTTAACACCGAATGGTCTACCGAGTTTACCCTGCTTAAAGCCTGGGATGAATACCTCAAAGCCTGGTTTGCCCTGCACCTTCTCg                                                  >  2:3343056/1‑100 (MQ=255)

                                                  ccGAAGACGAGTGCTTTAACACCGAATGGTCTACCGAGTTTGCCCTGTTTAAAGCCTGGGATGAATACCTCAAAGCCTGGTTTGCCCTGCACCTtctc                                                   <  2:2671434/98‑1 (MQ=255)

                                                    gAAGACGAGTGCTTTAACACCGAATGGTCTACCGAGTTTACCCTGTTTAAAGCCTGGGATGAATACCTCAAAGCCTGGTTTGCCCTGCACCTTCTCGAAg                                               <  1:1568227/100‑1 (MQ=255)

                                                     aaGACGAGTGCTTTAACACCGAATGGTCTACCGAGTTTACCCTGTTTAAAGCCTGGGATGAATACCTCAAAGCCTGGTTTGCCCTGCACCTTCTCGAAGc                                              >  1:1005740/1‑100 (MQ=255)

                                                     aaGACGAGTGCTTTAACACCGAATGGTCTACCGAGTTTACCCTGCTTAAAGCCTGGGATGAATACCTCAAAGCCTGGTTTGCCCTGCACCTTCTCGAAGc                                              <  1:3160226/100‑1 (MQ=255)

                                                      aGACGAGTGCTTTAACACCGAATGGTCTACCGAGTTTACCCTGTTTAAAGCCTGGGATGAATACCTCAAAGCCTGGTTTGCCCTGCACCTTCTCGAAGCg                                             <  2:1831547/100‑1 (MQ=255)

                                                      aGACGAGTGCTTTAACACCGAATGGTCTACCGAGTTTACCCTGTTTAAAGCCTGGGATGAATACCTCAAAGCCTGGTTTGCCCTGCACCTTCTCGAAGCg                                             <  1:531417/100‑1 (MQ=255)

                                                       gACGAGTGCTTTAACACCGAATGGTCTACCGAGTTTACCCTGTTTAAAGCCTGGGATGAATACCTCAAAGCCTGGTTTGCCCTGCACCTTCTCGAAGCGa                                            <  1:1899219/100‑1 (MQ=255)

                                                        aCGAGTGCTTTAACACCGAATGGTCTACCGAGTTTACCCTGCTTAAAGCCTGGGATGAATACCTCAAAGCCTGGTTTGCCCTGCACCTTCTCGAAGCGAt                                           <  2:948047/100‑1 (MQ=255)

                                                        aCGAGTGCTTTAACACCGAATGGTCTACCGAGTTTACCCTGCTTAAAGCCTGGGATGAATACCTCAAAGCCTGGTTTGCCCTGCACCTTCTCGAAGCGAt                                           >  2:3676502/1‑100 (MQ=255)

                                                          gAGTGCTTTAACACCGAATGGTCTACCGAGTTTACCCTGTTTAAAGCCTGGGATGAATACCTCAAAGCCTGGTTTGCCCTGCACCTTCTCGAAGCGATGt                                         >  2:1590546/1‑100 (MQ=255)

                                                             tGCTTTAACACCGAATGGTCTACCGAGTTTACCCTGCTTAAAGCCTGGGATGAATACCTCAAAGCCTGGTTTGCCCTGCACCTTCTCGAAGCGATGTTcc                                      <  1:831756/100‑1 (MQ=255)

                                                              gCTTTAACACCGAATGGTCTACCGAGTTTACCCTGTTTAAAGCCTGGGATGAATACCTCAAAGCCTGGTTTGCCCTGCACCTTCTCGAAGCGATGTTCCa                                     >  2:756154/1‑100 (MQ=255)

                                                              gCTTTAACACCGAATGGTCTACCGAGTTTACCCTGTTTAAAGCCTGGGATGAATACCTCAAAGCCTGGTTTGCCCTGCACATTCTCGACGCGATGTTCCa                                     >  2:4154005/1‑100 (MQ=255)

                                                               cTTTAACACCGAATGGTCTACCGAGTTTACCCTGTTTAAAGCCTGGGATGAATACCTCAAAGCCTGGTTTGCCCTGCACCTTCTCGAAGCGATGTTCCAg                                    <  1:2260435/100‑1 (MQ=255)

                                                               cTTTAACACCGAATGGTCTACCGAGTTTACCCTGTTTAAAGCCTGGGATGAATACCTCAAAGCCTGGTTTGCCCTGCACCTTCTCGAAGCGATGTTCCAg                                    >  1:1356464/1‑100 (MQ=255)

                                                                tttAACACCGAATGGTCTACCGAGTTTACCCTGTTTAAAGCCTGGGATGAATACCTCAAAGCCTGGTTTGCCCTGCACCTTCTCGAAGCGATGTTCCAGc                                   >  1:524948/1‑100 (MQ=255)

                                                                tttAACACCGAATGGTCTACCGAGTTTACCCTGCTTAAAGCCTGGGATGAATACCTCAAAGCCTGGTTTGCCCTGCACCTTCTCGAAGCGATGTTCCAGc                                   <  1:4096988/100‑1 (MQ=255)

                                                                 ttAACACCGAATGGTCTACCGAGTTTACCCTGTTTAAAGCCTGGGATGAATACCTCAAAGCCTGGTTTGCCCTGCACCTTCTCGAAGCGATGTTCCAGcc                                  >  2:1090720/1‑100 (MQ=255)

                                                                 ttAACACCGAATGGTCTACCGAGTTTACCCTGTTTAAAGCCTGGGATGAATACCTCAAAGCCTGGTTTGCCCTGCACCTTCTCGAAGCGATGTTCCAGcc                                  >  1:3633087/1‑100 (MQ=255)

                                                                 ttAACACCGAATGGTCTACCGAGTTTACCCTGTTTAAAGCCTGGGATGAATACCTCAAAGCCTGGTTTGCCCTGCACCTTCTCGAAGCGATGTTCCAGcc                                  <  1:3026179/100‑1 (MQ=255)

                                                                 ttAACACCGAATGGTCTACCGAGTATACCCTGTTTAAAGCCTGGGATGAATACCTCAAAGCCTGGTTTGCCCTGCACCTTCTCGAAGCGATGTTCCAGcc                                  <  2:180208/100‑1 (MQ=255)

                                                                  taccaacGAATGGTCTACCGAGTTTATCCTGTCTAGAGCCGGCGATTATTAAGTCAAAGCCTGGTTTGCCCTGCACCTTCTCGAAGCGATGTGCCAGCCt                                 <  2:2170360/94‑1 (MQ=255)

                                                                  tAACACCGAATGGTCTACCGAGTTTACCCTGTTTAAAGCCTGGGATGAATACCTCAAAGCCTGGTTTGCCCTGCACCTTCTCGAAGCGATGTTCCAGCCt                                 <  1:2107619/100‑1 (MQ=255)

                                                                   aaCACCGAATGGTCTACCGAGTTTACCCTGTTTAAAGCCTGGGATGAGTACCTCAAAGCCTGGTTTACCCTGCACCTTCTCGAAGCGATGTTCCAGCCtt                                >  2:134880/1‑100 (MQ=255)

                                                                   aaCACCGAATGGTCTACCGAGTTTACCCTGTTTAAAGCCTGGGATGAATACCTCAAAGCCTGGTTTGCCCTGCACCTTCTCGAAGCGATGTTCCAGCCtt                                >  2:2005162/1‑100 (MQ=255)

                                                                    acacCGAATGGTCTACCGAGTTTACCCTGTTTAAAGCCTGGGATGAATACCTCAAAGCCTGGTTTGCCCTGCACCTTCTCGAAGCGATGTTCCAGCCTTc                               <  2:3419540/100‑1 (MQ=255)

                                                                     cacCGAATGGTCTACCGAGTTTACCCTGTTTAAAGCCTGGGATGAATACCTCAAAGCCTGGTTTGCCCTGCACCTTCTCGAAGCGATGTTCCAGCCTTCt                              >  1:2166831/1‑100 (MQ=255)

                                                                      acCGAATGGTCTACCGAGTTTACCCTGTTTAAAGCCTGGGATGAATACCTCAAAGCCTGGTTTGCCCTGCACCTTCTCGAAGCGATGTTCCAGCCTTCTg                             <  2:3356442/100‑1 (MQ=255)

                                                                         gAATGGTCTACCGAGTTTACCCTGTTTAAAGCCTGGGATGAATACCTCAAAGCCTGGTTTGCCCTGCACCTTCTCGAAGCGATGTTCCAGCCTTCTGAtt                          >  1:1981443/1‑100 (MQ=255)

                                                                         gAATGGTCTACCGAGTTTACCCTGCTTAAAGCCTGGGATGAATACCTCAAAGCCTGGTTTGCCCTGCACCTTCTCGAAGCGATGTTCCAGCCTTCTGAtt                          >  1:2788060/1‑100 (MQ=255)

                                                                           aTGGTCTACCGAGTTTACCCTGTTTAAAGCCTGGGATGAATACCTCAAAGCCTGGTTTGCCCTGCACCTTCTCGAAGCGATGTTCCAGCCTTCTGATTcc                        <  2:2293825/100‑1 (MQ=255)

                                                                             ggTCTACCGAGTTTACCCTGTTTAAAGCCTGGGATGAATACCTCAAAGCCTGGTTTGCCCTGCACCTTCTCGAAGCGATGTTCCAGCCTTCTGATTCCgg                      <  2:3087478/100‑1 (MQ=255)

                                                                             ggTCTACCGAGTTTACCCTGTTTAAAGCCTGGGATGAATACCTCAAAGCCTGGTTTGCCCTGCACCTTCTCGAAGCGATGTTCCAGCCTTCTGATTCCgg                      >  1:2400024/1‑100 (MQ=255)

                                                                             ggTCTACCGAGTTTACCCTGCTTAAAGCCTGGGATGAATACCTCAAAGCCTGGTTTGCCCTGCACCTTCTCGAAGCGATGTTCCAGCCTTCTGATTCCgg                      >  2:2655035/1‑100 (MQ=255)

                                                                               tCTACCGAGTTTACCCTGTTTAAAGCCTGGGATGAATACCTCAAAGCCTGGTTTGCCCTGCACCTTCTCGAAGCGATGTTCCAGCCTTCTGATTCCGGTa                    <  1:1406371/100‑1 (MQ=255)

                                                                                cTACCGAGTTTACCCTGTTTAACGCCTGGGATGAATACCTCAAAGCCTGGTTTGCCCTTCACCTTCTCGAAGCGATGTTCCAGCCTTCTGATTCCGGTaa                   <  2:2617409/100‑1 (MQ=255)

                                                                                cTACCGAGTTTACCCTGTTTAAAGCCTGGGATGAATACCTCAAAGCCTGGTTTGCCCTGCACCTTCTCGAAGCGATGTTCCAGCCTTCTGATTCCGGTaa                   >  2:4057148/1‑100 (MQ=255)

                                                                                cTACCGAGTTTACCCTGTTTAAAGCCTGGGATGAATACCTCAAAGCCTGGTTTGCCCTGCACCTTCTCGAAGCGATGTTCCAGCCTTCTGATTCCGGTaa                   >  2:1656019/1‑100 (MQ=255)

                                                                                cTACCGAGTTTACCCTGCTTAAAGCCTGGGATGAATACCTCAAAGCCTGGTTTGCCCTGCACCTTCTCGAAGCGATGTTCCAGCCTTCTGATTCCGGTaa                   >  2:2970287/1‑100 (MQ=255)

                                                                                cTACCGAGTTTACCCTGCTTAAAGCCTGGGATGAATACCTCAAAGCCTGGTTTGCCCTGCACCTTCTCGAAGCGATGTTCCAGCCTTCTGATTCCGGTaa                   >  1:1947529/1‑100 (MQ=255)

                                                                                   ccGAGTTTACCCTGTTTAAAGCCTGGGATGAATACCTCAAAGCCTGGTTTGCCCTGCACCTTCTCGAAGCGATGTTCCAGCCTTCTGATTCCGGTAAATc                >  2:1198869/1‑100 (MQ=255)

                                                                                    cGAGTTTACCCTGCTTAAAGCCTGGGATGAATACCTCAAAGCCTGGTTTGCCCTGCACCTTCTCGAAGCGATGTTCCAGCCTTCTGATTCCGGTAAATCg               >  1:3916330/1‑100 (MQ=255)

                                                                                         ttACCCTGCTTAAAGCCTGGGATGAATACCTCAAAGCCTGGTTTGCCCTGCACCTTCTCGAAGCGATGTTCCAGCCTTCTGATTCCGGGAAATCGTTCAt          <  2:3197538/100‑1 (MQ=255)

                                                                                          tACCCTGTTTAAAGCCTGGGATGAATACCTCAAAGCCTGGTTTGCCCTGCACCTTCTCGAAGCGATGTTCCAGCCTTCTGATTCCGGTAAATCGTTCATc         >  2:3053314/1‑100 (MQ=255)

                                                                                          tACCCTGTTTAAAGCCTGGGATGAATACCTCAAAGCCTGGTTTGCCCTGCACCTTCTCGAAGCGATGTTCCAGCCTTCTGATTCCGGTAAATCGTTCATc         <  1:1485546/100‑1 (MQ=255)

                                                                                          tACCCTGCTTAAAGCCTGGGATGAATACCTCAAAGCCTGGTTTGCCCTGCACCTTCTCGAAGCGATGTTCCAGCCTTCTGATTCCGGTAAATCGTTCATc         >  1:1654844/1‑100 (MQ=255)

                                                                                           aCCCTGTTTAAAGCCTGGGATGAATACCTCAAAGCCTGGTTTGCCCTGCACCTTCTCGAAGCGATGTTCCAGCCTTCTGATTCCGGTAAATCGTTCATCt        <  1:185451/100‑1 (MQ=255)

                                                                                           aCCCTGTTTAAAGCCTGGGATGAATACCTCAAAGCCTGGTTTGCCCTGCACCTTCTCGAAGCGATGTTCCAGCCTTCTGATTCCGGTAAATCGTTCATCt        <  1:1104660/100‑1 (MQ=255)

                                                                                            cccTGTTTAAAGCCTGGGATGAATACCTCAAAGCCTGGTTTGCCCTGCACCTTCTCGAAGCGATGTTCCAGCCTTCTGATTCCGGTAAATCGTTCATCtt       <  1:1330882/100‑1 (MQ=255)

                                                                                            cccTGTTTAAAGCCTGGGATGAATACCTCAAAGCCTGGTTTGCCCTGCACCTTCTCGAAGCGATGTTCCAGCCTTCTGATTCCGGTAAATCGTTCATCtt       >  1:2548491/1‑100 (MQ=255)

                                                                                            cccTGTTTAAAGCCTGGGATGAATACCTCAAAGCCTGGTTTGCCCTGCACCTTCTCGAAGCGATGTTCCAGCCTTCTGATTCCGGTAAATCGTTCATCtt       <  2:2602457/100‑1 (MQ=255)

                                                                                            cccTGTTTAAAGCCTGGGATGAATACCTCAAAGCCTGGTTTGCCCTGCACCTTCTCGAAGCGATGTTCCAGCCTTCTGATTCCGGTAAATCGTTCATCtt       >  1:873395/1‑100 (MQ=255)

                                                                                            cccTGTTTAAAGCCTGGGATGAATACCTCAAAGCCTGGTTTGCCCTGCACCTTCTCGAAGCGATGGTCCAGCCTTCTGATTCCGGTAAATCGTTCATCt        >  2:148747/1‑99 (MQ=255)

                                                                                             ccTGTTTAAAGCCTGGGATGAATACCTCAAAGCCTGGTTTGCCCTGCACCTTCTCGAAGCGATGTTCCAGCCTTCTGATTCCGGTAAATCGTTCATCttt      <  1:1266198/100‑1 (MQ=255)

                                                                                             ccTGTTTAAAGCCTGGGATGAATACCTCAAAGCCTGGTTTGCCCTGCACCTTCTCGAAGCGATGTTCCAGCCTTCTGATTCCGGTAAATCGTTCATCttt      <  2:1028374/100‑1 (MQ=255)

                                                                                              cTGTTTAAAGCCTGGGATGAATACCTCAAAGCCTGGTTTGCCCTGCACCTTCTCGAAGCGATGTTCCAGCCTTCTGATTCCGGTAAATCGTTCATCTTTa     >  2:142858/1‑100 (MQ=255)

                                                                                                gCTTAAAGCCTGGGATGAATACCTCAAAGCCTGGTTTGCCCTGCACCTTCTCGAAGCGATGTTCCAGCCTTCTGATTCCGGTAAATCGTTCATCTTTAat   <  2:330024/100‑1 (MQ=255)

                                                                                                 cTTAAAGCCTGGGATGAATACCTCAAAGCCTGGTTTGCCCTGCACCTTCTCGAAGCGATGTTCCAGCCTTCTGATTCCGGTAAATCGTTCATCTTTAata  <  1:4078581/100‑1 (MQ=255)

AACCGTCCAAATTCTTGACCGCCTGGAGCTGGAAAAGCCCTGTATCGATGCCGAAGACGAGTGCTTTAACACCGAATGGTCTACCGAGTTTACCCTGCTTAAAGCCTGGGATGAATACCTCAAAGCCTGGTTTGCCCTGCACCTTCTCGAAGCGATGTTCCAGCCTTCTGATTCCGGTAAATCGTTCATCTTTAATA  >  NC_000913/3016308‑3016504

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 12 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A