Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A11 F56 I0 R1 215 141.2 6785521 95.3% 6466601 99.8

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 2,436,937 A→T 100% D305E (GAT→GAA)  pdxB ← erythronate‑4‑phosphate dehydrogenase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,436,9370AT96.5% 434.9 / 3.3 134D305E (GAT→GAA) pdxBerythronate‑4‑phosphate dehydrogenase
Reads supporting (aligned to +/- strand):  ref base A (2/3);  new base T (44/84);  total (46/88)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.85e-01

Only 100 of 136 total aligned reads displayed.

ATCGAACTCACCCGGTATCCCGGCGACTTTACGCAGCGGTGCGTCATCGCGGCGCACATCATACACCAAATGCACCAGCCTTTTCAGCGTCGGTT‑GATCGAGCGGGCCATGCAGCGTAATGCGACCAAACTCTGGCGCAGGCAGTAATGTATCCAGCGCAACGTGCTGTTCATGCCCAATAAACTTGCTATAA  >  NC_000913/2436841‑2437033
                                                                                                 |                                                                                                
aTCGAACTCACCCGGTATCCCGGCGACTTTACGCAGCGGTGCGTCATCGCGGCGCACATCATACACCAAATGCACCAGCCTTTTCAGCGTCGGTT‑GTTCg                                                                                               >  2:799019/1‑100 (MQ=255)
  cGAACTCACCCGGTATCCCGGCGACTTTACGCAGCGGTGCGTCATCGCGGCGCACATCATACACCAAATGCACCAGCCTTTTCAGCGTCGGTT‑GTTCGAg                                                                                             >  2:936771/1‑100 (MQ=255)
  cGAACTCACCCGGTATCCCGGCGACTTTACGCAGCGGTGCGTCATCGCGGCGCACATCATACACCAAATGCACCAGCCTTTTCAGCGTCGGTT‑GTTCGAg                                                                                             >  2:2513137/1‑100 (MQ=255)
   gAACTCACCCGGTATCCCGGCGACTTTACGCAGCGGTGCGTCATCGCGGCGCACATCATACACCAAATGCACCAGCCTTTTCAGCGTCGGTT‑GTTCGAGc                                                                                            >  2:2410555/1‑100 (MQ=255)
   gAACTCACCCGGTATCCCGGCGACTTTACGCAGCGGTGCGTCATCGCGGCGCACATCATACACCAAATGCACCAGCCTTTTCAGCGTCGGTT‑GTTCGAGc                                                                                            <  1:2743798/100‑1 (MQ=255)
      cTCACCCGGTATCCCGGCGACTTTACGCAGCGGTGCGTCATCGCGGCGCACATCATACACCAAATGCACCAGCCTTTTCAGCGTCGGTT‑GTTCGAGCggg                                                                                         <  2:742199/100‑1 (MQ=255)
      cTCACCCGGTATCCCGGCGACTTTACGCAGCGGTGCGTCATCGCGGCGCACATCATACACCAAATGCACCAGCCTTTTCAGCGTCGGTT‑GTTCGAGCggg                                                                                         <  2:2935472/100‑1 (MQ=255)
       tCACCCGGTATCCCGGCGACTTTACGCAGCGGTGCGTCATCGCGGCGCACATCATACACCAAATGCACCAGCCTTTTCAGCGTCGGTT‑GTTCGAGCGGGc                                                                                        <  1:3120167/100‑1 (MQ=255)
       tCACCCGGTATCCCGGCGACTTTACGCAGCGGTGCGTCATCGCGGCGCACATCATACACCAAATGCACCAGCCTTTTCAGCGTCGGTT‑GTTCGAGCGGGc                                                                                        <  2:3353629/100‑1 (MQ=255)
       tCACCCGGTATCCCGGCGACTTTACGCAGCGGTGCGTCATCGCGGCGCACATCATACACCAAATGCACCAGCCTTTTCAGCGTCGGTT‑GTTCGAGCGGGc                                                                                        <  2:462405/100‑1 (MQ=255)
        cACCCGGTATCCCGGCGACTTTACGCAGCGGTGCGTCATCGCGGCGCACATCATACACCAAATGCACCAGCCTTTTCAGCGTCGGTTAGATCggaagagc                                                                                        >  2:2904112/1‑93 (MQ=255)
        cACCCGGTATCCCGGCGACTTTACGCAGCGGTGCGTCATCGCGGCGCACATCATACACCAAATGCACCAGCCTTTTCAGCGTCGGTT‑GTTCGAGCGGGcc                                                                                       >  2:137319/1‑100 (MQ=255)
          cccGGTATCCCGGCGACTTTACGCAGCGGTGCGTCATCGCGGCGCACATCATACACCAAATGCACCAGCCTTTTCAGCGTCGGTT‑GTTCGAGCGGGCCAt                                                                                     <  2:1106068/100‑1 (MQ=255)
          cccGGTATCCCGGCGACTTTACGCAGCGGTGCGTCATCGCGGCGCACATCATACACCAAATGCACCAGCCTTTTCAGCGTCGGTT‑GTTCGAGCGGGCCAt                                                                                     <  1:3064907/100‑1 (MQ=255)
           ccGGTATCCCGGCGACTTTACGCAGCGGTGCGTCATCGCGGCGCACATCATACACCAAATGCACCAGCCTTTTCAGCGTCGGTT‑GTTCGAGCGGGCCATg                                                                                    <  1:2491982/100‑1 (MQ=255)
            cGGTATCCCGGCGACTTTACGCAGCGGTGCGTCATCGCGGCGCACATCATACACCAAATGCACCAGCCTTTTCAGCGTCGGTT‑GTTCGAGCGGGCCATGc                                                                                   <  1:1858858/100‑1 (MQ=255)
             ggTATCCCGGCGACTTTACGCAGCGGTGCGTCATCGCGGCGCACATCATACACCAAATGCACCAGCCTTTTCAGCGTCGGTT‑GTTCGAGCGGGCCATGCa                                                                                  <  2:2662401/100‑1 (MQ=255)
               tATCCCGGCGACTTTACGCAGCGGTGCGTCATCGCGGCGCACATCATACACCAAATGCACCAGCCTTTTCAGCGTCGGTT‑GTTCGAGCGGGCCATGCAGc                                                                                <  2:471548/100‑1 (MQ=255)
                 tCCCGGCGACTTTACGCAGCGGTGCGTCATCGCGGCGCACATCATACACCAAATGCACCAGCCTTTTCAGCGTCGGTT‑GTTCGAGCGGGCCATGCAGCGt                                                                              <  2:1290094/100‑1 (MQ=255)
                  cccGGCGACTTTACGCAGCGGTGCGTCATCGCGGCGCACATCATACACCAAATGCACCAGCCTTTTCAGCGTCGGTT‑GTTCGAGCGGGCCATGCAGCGTa                                                                             >  1:2274288/1‑100 (MQ=255)
                      gCGACTTTACGCAGCGGTGCGTCATCGCGGCGCACATCATACACCAAATGCACCAGCCTTTTCAGCGTCGGTT‑GTTCGAGCGGGCCATGCAGCGTAATg                                                                          >  1:1489335/1‑99 (MQ=255)
                      gCGACTTTACGCAGCGGTGCGTCATCGCGGCGCACATCATACACCAAATGCACCAGCCTTTTCAGCGTCGGTT‑GTTCGAGCGGGCCATGCAGCGTAATGc                                                                         <  2:1687356/100‑1 (MQ=255)
                       cGACTTTACGCAGCGGTGCGTCATCGCGGCGCACATCATACACCAAATGCACCAGCCTTTTCAGCGTCGGTT‑GTTCGAGCGGGCCATGCAGCGTAATGCg                                                                        <  2:271171/100‑1 (MQ=255)
                       cGACTTTACGCAGCGGTGCGTCATCGCGGCGCACATCATACACCAAATGCACCAGCCTTTTCAGCGTCGGTT‑GTTCGAGCGGGCCATGCAGCGTAATGCg                                                                        <  1:860704/100‑1 (MQ=255)
                       cGACTTTACGCAGCGGTGCGTCATCGCGGCGCACATCATACACCAAATGCACCAGCCTTTTCAGCGTCGGTT‑GTTCGAGCGGGCCATGCAGCGTAATGCg                                                                        <  1:3263741/100‑1 (MQ=255)
                         aCTTTATGAAGCGGTGCGTCATTGCGGCGCACATCATACACCAAATGCACCAGCCTTTTCAGCGTCGGTT‑GTTCGAGCGGGCCATGCAGCGTAATGc                                                                         <  2:297626/97‑1 (MQ=255)
                         aCTTTACGCAGCGGTGCGTCATCGCGGCGCACATCATACACCAAATGCACCAGCCTTTTCAGCGTCGGTT‑GTTCGAGCGGGCCATGCAGCGTAATGCGAc                                                                      <  1:1509961/100‑1 (MQ=255)
                         aCTTTACGCAGCGGTGCGTCATCGCGGCGCACATCATACACCAAATGCACCAGCCTTTTCAGCGTCGGTT‑GATCGAGCGGGCCATGCAGCGTAATGCGAc                                                                      <  1:3162857/100‑1 (MQ=255)
                          cTTTACGCAGCGGTGCGTCATCGCGGCGCACATCATACACCAAATGCACCAGCCTTTTCAGCGTCGGTT‑GTTCGAGCGGGCCATGCAGCGTAATGCGAcc                                                                     >  1:3342543/1‑100 (MQ=255)
                            ttACGCAGCGGTGCGTCATCGCGGCGCACATCATACACCAAATGCACCAGCCTTTTCAGCGTCGGTT‑GTTCGAGCGGGCCATGCAGCGTAATGCGACCaa                                                                   <  1:137319/100‑1 (MQ=255)
                             tACGCAGCGGTGCGTCATCGCGGCGCACATCATACACCAAATGCACCAGCCTTTTCAGCGTCGGTT‑GTTCGAGCGGGCCATGCAGCGTAATGCGACCaaa                                                                  >  1:1902116/1‑100 (MQ=255)
                              aCGCAGCGGTGCGTCATCGCGGCGCACATCATACACCAAATGCACCAGCCTTTTCAGCGTCGGTT‑GTTCGAGCGGGCCATGCAGCGTAATGCGACCAAAc                                                                 <  1:1443358/100‑1 (MQ=255)
                               cGCAGCGGTGCGTCATCGCGGCGCACATCATACACCAAATGCACCAGCCTTTTCAGCGTCGGTT‑GTTCGAGCGGGCCATGCAGCGTAATGCGACCAAAct                                                                >  1:317583/1‑100 (MQ=255)
                               cGCAGCGGTGCGTCATCGCGGCGCACATCATACACCAAATGCACCAGCCTTTTCAGCGTCGGTT‑GTTCGAGCGGGCCATGCAGCGTAATGCGACCAAAct                                                                >  1:2007494/1‑100 (MQ=255)
                                   gCGGTGCGTCATCGCGGCGCACATCATACTCCAAATGCACCAGCCTTTTCAGCGTCGGTT‑GTTCGAGCGGGCCATGCAGCGTAATGCGACCAAACTCTgg                                                            <  2:1832838/100‑1 (MQ=255)
                                   gCGGTGCGTCATCGCGGCGCACATCATACACCAAATGCACCAGCCTTTTCAGCGTCGGTT‑GTTCGAGCGGGCCATGCAGCGTAATGCGACCAAACTCTgg                                                            <  2:2415861/100‑1 (MQ=255)
                                    cGGTGCGTCATCGCGGCGCACATCATACACCAAATGCACCAGCCTTTTCAGCGTCGGTT‑GTTCGAGCGGGCCAGGCAGCGTAATGCGACCAAACTCTGgc                                                           <  1:1343783/100‑1 (MQ=255)
                                     ggTGCGTCATCGCGGCGCACATCATACACCAAATGCACCAGCCTTTTCAGCGTCGGTT‑GTTCGAGCGGGCCATGCAGCGTAATGCGACCAAACTCTGgcg                                                          <  1:5107/100‑1 (MQ=255)
                                      gTGCGTCATCGCGGCGCACATCATACACCAAATGCACCAGCCTTTTCAGCGTCTGTT‑GTTCGAGCGGGCCATGCAGCGTAATGCGACCAATCTCTGgcgc                                                         <  1:1872479/100‑1 (MQ=255)
                                      gTGCGTCATCGCGGCGCACATCATACACCAAATGCACCAGCCTTTTCAGCGTCGGTT‑GTTCGAGCGGGCCATGCAGCGTAATGCGACCAAACTCTGgcgc                                                         <  2:1670233/100‑1 (MQ=255)
                                       tGCGTCATCGCGGCGCACATCATACACCAAATGCACCAGCCTTTTCAGCGTCGGTT‑GTTCGAGCGGGCCATGCAGCGTAATGCGACCAAACTCTGGCgca                                                        <  2:2081131/100‑1 (MQ=255)
                                        gCGTCATCGCGGCGCACATCATACACCAAATGCACCAGCCTTTTCAGCGTCGGTT‑GTTCGAGCGGGCCATGCAGCGTAATGCGACCAAACTCTGGCgcag                                                       <  2:3342571/100‑1 (MQ=255)
                                        gCGTCATCGCGGCGCACATCATACACCAAATGCACCAGCCTTTTCAGCGTCGGTT‑GTTCGAGCGGGCCATGCAGCGTAATGCGACCAAACTCTGGCgcag                                                       <  2:2805734/100‑1 (MQ=255)
                                         cGTCATCGCGGCGCACATCATACACCAAATGCGCCAGCCTTTTCAGCGTCGGTT‑GTTCGAGCGGGCCATGCAGCGTAATGCGACCAAACTCTGGCgcagg                                                      >  2:2886516/1‑100 (MQ=255)
                                          gTCATCGCGGCGCACATCATACACCAAATGCACCAGCCTTTTCAGCGTCGGTT‑GTTCGAGCGTGCCATGCAGCGTAATGCGACCAAACTCTGGCgcaggc                                                     <  1:1810555/100‑1 (MQ=255)
                                          gTCATCGCGGCGCACATCATACACCAAATGCACCAGCCTTTTCAGCGTCGGTT‑GTTCGAGCGGGCCATGCAGCGTAATGCGACCAAACTCTGGCgcaggc                                                     <  2:758357/100‑1 (MQ=255)
                                            cATCGCGGCGCACATCATACACCGAATGCACCAGCCTTTTCAGCGTCGGTT‑GTTCGAGCGGGCCATGCAGCGTAATGCGACCAAACTCTGGCgcaggcag                                                   <  1:3079/100‑1 (MQ=255)
                                            cATCGCGGCGCACATCATACACCAAATGCACCAGCCTTTTCAGCGTCGGTT‑GTTCGAGCGGGCCATGCAGCGTAATGCGACCAAACTCTGGCgcaggcag                                                   >  2:2510081/1‑100 (MQ=255)
                                            cATCGCGGCGCACATCATACACCAAATGCACCAGCCTTTTCAGCGTCGGTT‑GTTCGAGCGGGCCATGCAGCGTAATGCGACCAAACTCTGGCgcaggcag                                                   >  1:288040/1‑100 (MQ=255)
                                            cATCGCGGCGCACATCATACACCAAATGCACCAGCCTTTTCAGCGTCGGTT‑GTTCGAGCGGGCCATGCAGCGTAATGCGACCAAACTCTGGCgcaggcag                                                   <  2:2265835/100‑1 (MQ=255)
                                            cATCGCGGCGCACATCATACACCAAATGCACCAGCCTTTTCAGCGTCGGTT‑GATCGAGCGGGCCATGCAGCGTAATGCGACCAAACTCTGGCgcaggcag                                                   >  2:321053/1‑100 (MQ=255)
                                             aTCGCGGCGCACATCATACACCAAATGCACCAGCCTTTTCAGCGTCGGTT‑GTTCGAGCGGGCCATGCAGCGTAATGCGACCAAACTCTGGCGCAGGCAGt                                                  >  1:1411642/1‑100 (MQ=255)
                                             aTCGCGGCGCACATCATACACCAAATGCACCAGCCTTTTCAGCGTCGGTT‑GTTCGAGCGGGCCATGCAGCGTAATGCGACCAAACTCTGGCGCAGGCAGt                                                  >  1:852547/1‑100 (MQ=255)
                                               cgcgGCGCACATCATACACCAAATGCACCAGCCTTTTCAGCGTCGGTT‑GTTCGAGCGGGCCATGCAGCGTAATGCGACCAAACTCTGGCGCAGGCAGTaa                                                >  1:2683438/1‑100 (MQ=255)
                                                 cggcgCACATCATACACCAAATGCACCAGCCTTTTCAGCGTCGGTT‑GTTCGAGCGGGCCATGCAGCGTAATGCGACCAAACTCTGGCGCAGGCAGTAATg                                              <  1:2863133/100‑1 (MQ=255)
                                                 cggcgCACATCATACACCAAATGCACCAGCCTTTTCAGCGTCGGTT‑GTTCGAGCGGGCCATGCAGCGTAATGCGACCAAACTCTGGCGCAGGCAGTAATg                                              <  2:3207406/100‑1 (MQ=255)
                                                    cgcACATCATACACCAAATGCACCAGCCTTTTCAGCGTCGGTT‑GTTCGAGCGGGCCATGCAGCGTAATGCGACCAAACTCTGGCGCAGGCAGTAATGTAt                                           <  1:2852679/100‑1 (MQ=255)
                                                        catcatACACCAAATGCACCAGCCTTTTCAGCGTCGGTT‑GTTCGAGCGGGCCATGCAGCGTAATGCGACCAAACTCTGGCGCAGGCAGTAATGTATCCAg                                       >  1:747942/1‑100 (MQ=255)
                                                             tACACCAAATGCACCAGCCTTTTCAGCGTCGGTT‑GTTCGAGCGGGCCATGCAGCGTAATGCGACCAAACTCTGGCGCAGGCAGTAATGTATCCAGCGCaa                                  >  2:1074099/1‑100 (MQ=255)
                                                             tACACCAAATGCACCAGCCTTTTCAGCGTCGGTT‑GTTCGAGCGGGCCATGCAGCGTAATGCGACCAAACTCTGGCGCAGGCAGTAATGTATCCAGCGCaa                                  >  1:554422/1‑100 (MQ=255)
                                                               cacCAAATGCACCAGCCTTTTCAGCGTCGGTT‑GTTCGAGCGGGCCATGCAGCGTAATGCGACCAAACTCTGGCGCAGGCAGTAATGTATCCAGCGCAACg                                >  1:1589545/1‑100 (MQ=255)
                                                               cacCAAATGCACCAGCCTTTTCAGCGTCGGTT‑GTTCGAGCGGGCCATGCAGCGTAATGCGACCAAACTCTGGCGCAGGCAGTAATGTATCCAGCGCAACg                                >  2:2713140/1‑100 (MQ=255)
                                                               cacCAAATGCACCAGCCTTTTCAGCGTCGGTT‑GTTCGAGCGGGCCATGCAGCGTAATGCGACCAAACTCTGGCGCAGGCAGTAATGTATCCAGCGCAACg                                >  1:999376/1‑100 (MQ=255)
                                                                acCAAATGCACCAGCCTTTTCAACGTCGGTT‑GTTCGAGCGGGCCATGCAGCGTAATGCGACCAAACTCTGGCGCAGGCAGTAATGTATCCAGTGCAACGt                               >  1:771711/1‑100 (MQ=255)
                                                                  cAAATGCACCAGCCTTTTCAGCGTCGGTT‑GTTCGAGCGGGCCATGCAGCGTAATGCGACCAAACTCTGGCGCAGGCAGTAATGTATCCAGCGCAACGTGc                             <  1:1766989/100‑1 (MQ=255)
                                                                  cAAATGCACCAGCCTTTTCAGCGTCGGTT‑GTTCGAGCGGGCCATGCAGCGTAATGCGACCAAACTCTGGCGCAGGCAGTAATGTATCCAGCGCAACGTGc                             <  2:2338496/100‑1 (MQ=255)
                                                                  cAAATGCACCAGCCTTTTCAGCGTCGGTT‑GTTCGAGCGGGCCATGCAGCGTAATGCGACCAAACTCTGGCGCAGGCAGTAATGTATCCAGCGCAACGTGc                             <  1:2720184/100‑1 (MQ=255)
                                                                    aaTGCACCAGCCTTTTCAGCGTCGGTT‑GTTCGAGCGGGCCATGCAGCGTAATGCGACCAAACTCTGGCGCAGGCAGTAATGTATCCAGTGCAACGTGCTg                           <  2:1925830/100‑1 (MQ=255)
                                                                      atctccAGCCTTTTCAGCGTCGGTT‑GTTCGAGCGGGCCATGCAGCGTAATGCGACCAAACTCTGGCGCAGGCAGTAATGTATCCAGCGCAACGTGCTGtt                         <  1:2759463/96‑1 (MQ=255)
                                                                       gCACCAGCCTTTTCAGCGTCGGTT‑GTTCGAGCGGGCCATGCAGCGTAATGCGACCAAACTCTGGCGCAGGCAGTAATGTATCCAGCGCAACGTGCTGTTc                        >  2:2510815/1‑100 (MQ=255)
                                                                          ccAGCCTTTTCAGCGTCGGTT‑GTTCGAGCGGGCCATGCAGCGTAATGCGACCAAACTCTGGCGCAGGCAGTAATGTATCCAGCGCAACGTGCTGttagat                     >  2:2759486/1‑96 (MQ=255)
                                                                          ccAGCCTTTTCAGCGTCGGTT‑GTTCGAGCGGGCCATGCAGCGTAATGCGACCAAACTCTGGCGCAGGCAGTAATGTATCCAGCGCAACGTGCTGTTCATg                     <  1:3385634/100‑1 (MQ=255)
                                                                          ccAGCCTTTTCAGCGTCGGTT‑GTTCGAGCGGGCCATGCAGCGTAATGCGACCAAACTCTGGCGCAGGCAGTAATGTATCCAGCGCAACGTGCTGTTCATg                     <  2:33023/100‑1 (MQ=255)
                                                                            aGCCTTTTCAGCGTCGGTT‑GTTCGAGCGGGCCATGCAGCGTAATGCGACCAAACTCTGGCGCAGGCAGTAATGTATCCAGCGCAACGTGCTGTTCATGcc                   >  1:185757/1‑100 (MQ=255)
                                                                             gCCTTTTCAGCGTCGGTT‑GTTCGAGCGGGCCATGCAGCGTAATGCGACCAAACTCTGGCGCAGGCAGTAATGTATCCAGCGCAACGTGCTGTTCagatcg                  >  2:1416495/1‑95 (MQ=255)
                                                                             gCCTTTTCAGCGTCGGTT‑GTTCGAGCGGGCCATGCAGCGTAATGCGACCAAACTCTGGCGCAGGCAGTAATGTATCCAGCGCAACGTGCTGTTCATGccc                  <  1:657357/100‑1 (MQ=255)
                                                                             gCCTTTTCAGCGTCGGTT‑GTTCGAGCGGGCCATGCAGCGTAATGCGACCAAACTCTGGCGCAGGCAGTAATGTATCCAGCGCAACGTGCTGTTCATGccc                  <  2:1588294/100‑1 (MQ=255)
                                                                             gCCTGTTCAGCGTCGGTG‑ATTCGGGGGAGGCATGCGGCGGAATGCGACCAAACTCTGGCGCAGGAAGTAATGTTTCCAGCGCAACGTGCTGTTCa                       <  1:2054879/95‑1 (MQ=255)
                                                                              ccTTTTCAGCGTCGGTT‑GTTCGAGCGGGCCATGCAGCGTAATGCGACCAAACTCTGGCGCAGGCAGTAATGTATCCAGCGCAACGTGCTGTTCATGCCCa                 <  1:2410537/100‑1 (MQ=255)
                                                                               cTTTTCAGCGTCGGTT‑GTTCGAGCGGGCCATGCAGCGTAATGCGACCAAACTCTGGCGCAGGCAGTAATGTATCCAGCGCAACGTGCTGTTCATGCCCaa                >  2:2235027/1‑100 (MQ=255)
                                                                                ttttCAGCGTCGGTT‑GTTCGAGCGGGCCATGCAGCGTAATGCGACCAAACTCTGGCGCAGGCAGTAATGTATCCAGCGCAACGTGCTGTTCATGCCCAAt               >  2:346114/1‑100 (MQ=255)
                                                                                ttttCAGCGTCGGTT‑GTTCGAGCGGGCCATGCAGCGTAATGCGACCAAACTCTGGCGCAGGCAGTAATGTATCCAGCGCAACGTGCTGTTCATGCCCAAt               <  1:2494966/100‑1 (MQ=255)
                                                                                tccgatctgTCGGTT‑GTTCGAGCGGGCCATGCAGCGTAATGCGACCAAACTCTGGCGCAGGCAGTAATGTATCCAGCGCAACGTGCTGTTCATGCCCAAt               <  2:324683/92‑1 (MQ=255)
                                                                                 tttCAGCGTCGGTT‑GTTCGAGCGGGCCATGCAGCGTAATGCGACCAAACTCTGGCGCAGGCAGTAATGTATCCAGCGCAACGTGCTGTTCATGCCCAATa              <  2:2821032/100‑1 (MQ=255)
                                                                                    cAGCGTCTGTT‑GATCGCCCGGGCCATGCAGCGTACTGCGACCAAACTCTGGCGCAAGCAGTAATGTATCCAGCGCAACGTGCTGTTCATGCCCAATaa             <  1:3310965/98‑1 (MQ=255)
                                                                                    cAGCGTCGGTT‑GTTCGAGCGGGCCATGCAGCGTAATGCGACCAAACTCTGGCGCAGGCAGTAATGTATCCAGCGCAACGTGCTGTTCATGCCCAATAAAc           >  1:1948920/1‑100 (MQ=255)
                                                                                     aGCGTCGGTT‑GTTCGAGCGGGCCATGCAGCGTAATGCGACCAAACTCTGGCGCAGGCAGTAATGTATCCAGCGCAACGTGCTGTTCATGCCCAATAAACt          <  1:3079800/100‑1 (MQ=255)
                                                                                     aGCGTCGGTT‑GTTCGAGCGGGCCATGCAGCGTAATGCGACCAAACTCTGGCGCAGGCAGTAATGTATCCAGCGCAACGTGCTGTTCATGCCCAATAAACt          <  2:524808/100‑1 (MQ=255)
                                                                                      gCGTCGGTT‑GTTCGAGCGGGCCATGCAGCGTAATGCGACCAAACTCTGGCGCAGGCAGTAATGTATCCAGCGCAACGTGCTGTTCATGCCCAATAAACtt         >  2:1375430/1‑100 (MQ=255)
                                                                                        gTCGGTT‑GTTCGAGCGGGCCATGCAGCGTAATGCGACCAAACTCTGGCGCAGGCAGTAATGTATCCAGCGCAACGTGCTGTTCATGCCCAATagatcgga       >  1:324682/1‑93 (MQ=255)
                                                                                        gTCGGTT‑GTTCGAGCGGGCCATGCAGCGTAATGCGACCAAACTCTGGCGCAGGCAGTAATGTATCCAGCGCAACGTGCTGTTCATGCCCAATAAACTTg        >  1:2792063/1‑99 (MQ=255)
                                                                                        gTCGGTT‑GTTCGAGCGGGCCATGCAGCGTAATGCGACCAAACTCTGGCGCAGGCAGTAATGTATCCAGCGCAACGTGCTGTTCATGCCCAATAAACTTGc       <  2:1770405/100‑1 (MQ=255)
                                                                                         tCGGTT‑GTTCGAGCGGGCCATGCAGCGTAATGCGACCAAACTCTGGCGCAGGCAGTAATGTATCCAGCGCAACGTGCTGTTCATGCCCAATAAACTTGCt      <  1:936768/100‑1 (MQ=255)
                                                                                           ggTT‑GTTCGAGCGGGCCATGCAGCGTAATGCGACCAAACTCTGGCGCAGGCAGTAATGTATCCAGCGCAACGTGCTGTTCATGCCCAATAAACTTGCtat    >  2:2634542/1‑100 (MQ=255)
                                                                                           ggTT‑GTTCGAGCGGGCCATGCAGCGTAATGCGACCAAACTCTGGCGCAGGCAGTAATGTATCCAGCGCAACGTGCTGTTCATGCCCAATAAACTTGCtat    >  2:497497/1‑100 (MQ=255)
                                                                                           ggTT‑GTTCGAGCGGGCCATGCAGCGTAATGCGACCAAACTCTGGCGCAGGCAGTAATGTATCCAGCGCAACGTGCTGTTCATGCCCAATAAACTTGCtat    <  1:970234/100‑1 (MQ=255)
                                                                                            gtt‑gttCGAGCGGGCCATGCAGCGTAATGCGACCAAACTCTGGCGCCGGCAGTAATGTATCCGGCGCAAAGTGCTGTTCATGCCCAATAAACTTGCta     >  2:2986700/1‑98 (MQ=255)
                                                                                            gtt‑gttCGAGCGGGCCATGCAGCGTAATGCGACCAAACTCTGGCGCAGGCAGTAATGTATCCAGCGCAACGTGCTGTTCATGCCCAATAAACTTGCtata   >  1:580720/1‑100 (MQ=255)
                                                                                            gTT‑GATCGAGCGGGCCATGCAGCGTAATGCGACCAAACTCTGGCGCAGGCAGTAATGTATCCAGCGCAACGTGCTGTTCATGCCCACTAAACTTGCtaga   <  2:2295128/100‑3 (MQ=255)
                                                                                             tt‑GTTCGAGCGGGCCATGCAGCGTAATGCGACCAAACTCTGGCGCAGGCAGTAATGTATCCAGCGCAACGTGCTGTTCATGCCCAATAAACTTGCTATaa  >  2:2398775/1‑100 (MQ=255)
                                                                                                 |                                                                                                
ATCGAACTCACCCGGTATCCCGGCGACTTTACGCAGCGGTGCGTCATCGCGGCGCACATCATACACCAAATGCACCAGCCTTTTCAGCGTCGGTT‑GATCGAGCGGGCCATGCAGCGTAATGCGACCAAACTCTGGCGCAGGCAGTAATGTATCCAGCGCAACGTGCTGTTCATGCCCAATAAACTTGCTATAA  >  NC_000913/2436841‑2437033

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A