Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A11 F256 I0 R1 328 102.7 5123138 93.4% 4785010 99.8

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 2,436,937 A→T 100% D305E (GAT→GAA)  pdxB ← erythronate‑4‑phosphate dehydrogenase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,436,9370AT96.7% 292.0 / 0.5 91D305E (GAT→GAA) pdxBerythronate‑4‑phosphate dehydrogenase
Reads supporting (aligned to +/- strand):  ref base A (3/0);  new base T (37/51);  total (40/51)
Fisher's exact test for biased strand distribution p-value = 8.13e-02
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.45e-01

ATCGAACTCACCCGGTATCCCGGCGACTTTACGCAGCGGTGCGTCATCGCGGCGCACATCATACACCAAATGCACCAGCCTTTTCAGCGTCGGTTGATCGAGCGGGCCATGCAGCGTAATGCGACCAAACTCTGGCGCAGGCAGTAATGTATCCAGCGCAACGTGCTGTTCATGCCCAATAAACTTGCTATAA  >  NC_000913/2436841‑2437033
                                                                                                |                                                                                                
aTCGAACTCACCCGGTATCCCGGCGACTTTACGCAGCGGTGCGTCATCGCGGCGCACATCATACACCAAATGCACCAGCCTTTTCAGCGTCGGTTGTTCg                                                                                               >  1:2151534/1‑100 (MQ=255)
 tCGAACTCACCCGGTATCCCGGCGACTTTACGCAGCGGTGCGTCATCGCGGCGCACATCATACACCAAATGCACCAGCCTTTTCAGCGTCGGTTGTTCGa                                                                                              >  2:2140415/1‑100 (MQ=255)
  cGAACTCACCCGGTATCCCGGCGACTTTACGCAGCGGTGCGTCATCGCGGCGCACATCATACACCAAATGCACCAGCCTTTTCAGCGTCGGTTGTTCGAg                                                                                             <  2:492344/100‑1 (MQ=255)
   gAACTCACCCGGTATCCCGGCGACTTTACGCAGCGGTGCGTCATCGCGGCGCACATCATACACCAAATGCACCAGCCTTTTCAGCGTCGGTTGTTCGAGc                                                                                            <  1:528915/100‑1 (MQ=255)
      cTCACCCGGTATCCCGGCGACTTTACGCAGCGGTGCGTCATCGCGGCGCACATCATACACCAAATGCACCAGCCTTTTCAGCGTCGGTTGTTCGAGCggg                                                                                         >  2:337899/1‑100 (MQ=255)
       tCACCCGGTATCCCGGCGACTTTACGCAGCGGTGCGTCATCGCGGCGCACATCATACACCAAATGCACCAGCCTTTTCAGCGTCGGTTGTTCGAGCGGGc                                                                                        <  1:1371033/100‑1 (MQ=255)
       tCACCCGGTATCCCGGCGACTTTACGCAGCGGTGCGTCATCGCGGCGCACATCATACACCAAATGCACCAGCCTTTTCAGCGTCGGTTGTTCGAGCGGGc                                                                                        <  1:1421069/100‑1 (MQ=255)
        cACCCGGTATCCCGGCGACTTTACGCAGCGGTGCGTCATCGCGGCGCACATCATACACCAAATGCACCAGCCTTTTCAGCGTCGGTTGTTCGAGCGGGcc                                                                                       >  2:957600/1‑100 (MQ=255)
        cACCCGGTATCCCGGCGACTTTACGCAGCGGTGCGTCATCGCGGCGCACATCATACACCAAATGCACCAGCCTTTTCAGCGTCGGTTGTTCGAGCGGGcc                                                                                       >  1:602352/1‑100 (MQ=255)
         aCCCGGTATCCCGGCGACTATACGCAGCGGTGCGTTATCGCGGCGCACATCATACACCAAAGGCACCAGCCTTTTCAGCGTCGGTTGTTCGAGCGAGCCa                                                                                      <  2:1676377/100‑1 (MQ=255)
          cccGGTATCCCGGCGACTTTACGCAGCGGTGCGTCATCGCGGCGCACATCATACACCAAATGCACCAGCCTTTTCAGCGTCGGTTGTTCGAGCGGGCCa                                                                                      <  1:2173922/99‑1 (MQ=255)
          cccGGTATCCCGGCGACTTTACGCAGCGGTGCGTCATCGCGGCGCACACTATACACCAAATGCACCAGCCTTTTCAGCGTCGGTTGTTCGAGCGGGCCAt                                                                                     >  2:1965668/1‑100 (MQ=255)
              gTATCCCGGCGACTTTACGCAGAGGTGCGTCATCGCGTCGCACATCATACACCAAATGCACCAGCCTTTTCAGCGTCGGTTGTTCGAGCGGGCCATGCAg                                                                                 <  2:1733659/100‑1 (MQ=255)
                aTCCCGGCGACTTTACGCAGCGGTGCGTCATCGCGGCGCACATCATACACCAAATGCACCAGCCTTTTCAGCGTCGGTTGTTCGAGCGGGCCATGCAGCg                                                                               <  2:1042529/100‑1 (MQ=255)
                aTCCCGGCGACTTTACGCAGCGGTGCGTCATCGCGGCGCACATCATACACCAAATGCACCAGCCTTTTCAGCGTCGGTTGTTCGAGCGGGCCATGCAGCg                                                                               >  2:2014841/1‑100 (MQ=255)
                 tCCCGGCGACTTTACGCAGCGGTGCGTCATCGCGGCGCACATCATACACCAAATGCACCAGCCTTTTCAGCGTCGGTTGTTCGAGCGGGCCATGCAGCGt                                                                              >  2:749115/1‑100 (MQ=255)
                 tCCCGGCGACTTTACGCAGCGGTGCGTCATCGCGGCGCACATCATACACCAAATGCACCAGCCTTTTCAGCGTCGGTTGTTCGAGCGGGCCATGCAGCGt                                                                              <  1:395598/100‑1 (MQ=255)
                  cccGGCGACTTTACGCAGCGGTGCGTCATCGCGGCGCACATCATACACCAAATGCACCAGCCTTTTCAGCGTCGGTTGTTCGAGCGGGCCATGCAGCGTa                                                                             >  2:2067014/1‑100 (MQ=255)
                  cccGGCGACTTTACGCAGCGGTGCGTCATCGCGGCGCACATCATACACCAAATGCACCAGCCTTTTCAGCGTCGGTTGTTCGAGCGGGCCATGCAGCGTa                                                                             >  1:761312/1‑100 (MQ=255)
                   ccGGCGACTTTACGCAGCGGTGCGTCATCGCGGCGCACATCATACACCAAATGCACCAGCCTTTTCAGCGTCGGTTGTTCGAGCGGGCCATGCAGCGTa                                                                             >  2:1748588/1‑99 (MQ=255)
                   ccGGCGACTTTACGCAGCGGTGCGACATCGCGGCGCACATCATACACCAAATGCACCAGCCTTTTCAGCGTCGGTTGTTCGAGCGGGCCATGCAGCGTaa                                                                            >  1:1805388/1‑100 (MQ=255)
                     ggCGACTTTACGCAGCGGTGCGTCATCGCGGCGCACATCATACACCAAATGCACCAGCCTTTTCAGCGTCGGTTGTTCGAGCGGGCCATGCAGCGTAATg                                                                          <  2:792028/100‑1 (MQ=255)
                     ggCGACTTTACGCAGCGGTGCGTCATCGCGGCGCACATCATACACCAAATGCACCAGCCTTTTCAGCGTCGGTTGTTCGAGCGGGCCATGCAGCGTAATg                                                                          <  1:1416654/100‑1 (MQ=255)
                       cGACTTTACGCAGCGGTGCGTCATCGCGGCGCACATCATACACCAAATGCACCAGCCTTTTCAGCGTCGGTTGTTCGAGCGGGCCATGCAGCGTAATGCg                                                                        <  1:2208180/100‑1 (MQ=255)
                            ttACGCAGCGGTGCGTCATCGCGGCGCACATCATACACCAAATGCACCAGCCTTTTCAGCGTCGGTTGTTCGAGCGGGCCATGCAGCGTAATGCGACCaa                                                                   >  1:821110/1‑100 (MQ=255)
                              aCGCAGCGGTGCGTCATCGCGGCGCACATCATACACCAAATGCACCAGCCTTTTCAGCGTCGGTTGTTCGAGCGGGCCATGCAGCGTAATGCGACCAAAc                                                                 <  1:2055045/100‑1 (MQ=255)
                                  aGCGGTGCGTCATCGCGGCGCACATCATACACCAAATGCACCAGCCTTTTCAGCGTCGGTTGTTCGAGCGGGCCATGCAGCGTAATGCGACCAAACTCTg                                                             <  1:1826007/100‑1 (MQ=255)
                                   gCGGTGCGTCATCGCGGCGCACATCATACACCAACTGCACCAGCCTTTTCAGCGTCGGTTGTTCGAGCGGGCCATGCAGCGTAATGCGACCAAACTCTgg                                                            >  2:1159320/1‑100 (MQ=255)
                                    cGGTGCGTCATCGCGGCGCACATCATACACCAAGTGCACCAGCCTTTTCAGCGTCGGTTGTTCGAGCGGGCCATGCAGCGTAATGCGACCAAACTCTGgc                                                           <  2:2278844/100‑1 (MQ=255)
                                    cGGTGCGTCATCGCGGCGCACATCATACACCAAATGCACCAGCCTTTTCAGCGTCGGTTGTTCGAGCGGGCCATGCAGCGTAATGCGACCAAACTCTGgc                                                           <  1:81893/100‑1 (MQ=255)
                                     ggTGCGTCATCGCGGCGCACATCTTACACCAAATGCACCAGCCTTTTCAGCGTCGGTTGTTCGAGCGGGCCATGCAGCGTAATGCGACCAAACTCTGgcg                                                          <  1:378309/100‑1 (MQ=255)
                                     ggTGCGTCATCGCGGCGCACATCATACACCAAATGCACCAGCCTTTTCAGCGTCGGTTGTTCGAGCGGGCCATGCAGCGTAATGCGACCAAACTCTGgcg                                                          >  1:2067419/1‑100 (MQ=255)
                                        gCGTCATCGCGGCGCACATCATACACCAAATGCACCAGCCTTTTCAGCGTCGGTTGTTCGAGCGGGCCATGCAGCGTAATGCGACCAAACTCTGGCgcag                                                       <  1:1283573/100‑1 (MQ=255)
                                        gCGTCATCGCGGCGCACATCATACACCAAATGCACCAGCCTTTTCAGCGTCGGTTGTTCGAGCGGGCCATGCAGCGTAATGCGACCAAACTCTGGCgcag                                                       >  1:1251783/1‑100 (MQ=255)
                                        gCGTCATCGCGGCGCACATCATACACCAAATGCACCAGCCTTTTCAGCGTCGGTTGTTCGAGCGGGCCATGCAGCGTAATGCGACCAAACTCTGGCgcag                                                       >  1:2107944/1‑100 (MQ=255)
                                        gCGTCATCGCGGCGCACATCATACACCAAATGCACCAGCCTTTTCAGCGTCGGTTGATCGAGCGGGCCATGCAGCGTAATGCGACCAAACTCTGGCgcag                                                       >  2:2347522/1‑100 (MQ=255)
                                         cGTCATCGCGGCGCACATCATACACCAAATGCACCAGCCTTTTCAGCGTCGGTTGTTCGAGCGGGCCATGCAGCGTAATGCGACCAAACTCTGGCgcagg                                                      <  1:1377326/100‑1 (MQ=255)
                                          gTCATCGCGGCGCACATCATACACCAAATGCACCAGCCTTTTCAGCGTCGGTTGTTCGAGCGGGCCATGCAGCGTAATGCGACCAAACTCTGGCgcaggc                                                     >  2:690575/1‑100 (MQ=255)
                                            cATCGCGGCGCACATCATACACCAAATGCACCAGCCTTTTCAGCGTCGGTTGTTCGAGCGGGCCATGCAGCGTAATGCGACCAAACTCTGGCgcaggcag                                                   <  1:1953900/100‑1 (MQ=255)
                                             aTCGCGGCGCACATCATACACCAAATGCACCAGCCTTTTCAGCGTCGGTTGTTCGAGCGGGCCATGCAGCGTAATGCGACCAAACTCTGGCGCAGGCAGt                                                  >  2:1920688/1‑100 (MQ=255)
                                                 cggcgCACATCATACACCAAATGCACCAGCCTTTTCAGCGTCGGTTGTTCGAGCGGGCCATGCAGCGTAATGCGACCAAACTCTGGCGCAGGCAGTAATg                                              <  2:1940002/100‑1 (MQ=255)
                                                 cggcgCACATCATACACCAAATGCACCAGCCTTTTCAGCGTCGGTTGTTCGAGCGGGCCATGCAGCGTAATGCGACCAAACTCTGGCGCAGGCAGTAATg                                              <  2:1889266/100‑1 (MQ=255)
                                                   gcgcACATCATACACCAAATGCACCAGCCTTTTCAGCGTCGGTTGTTCGAGCGGGCCATGCAGCGTAATGCGACCAAACTCTGGCGCAGGCAGTAATGTa                                            <  2:1858179/100‑1 (MQ=255)
                                                    cgcACATCATACACCAAATGCACCAGCCTTTTCAGCGTCGGTTGTTCGAGCGGGCCATGCAGCGTAATGCGACCAAACTCTGGCGCAGGCAGTAATGTa                                            <  2:510568/99‑1 (MQ=255)
                                                    cgcACATCATACACCAAATGCACCAGCCTTTTCAGCGTCGGTTGTTCGAGCGGGCCATGCAGCGTAATGCGACCAAACTCTGGCGCAGGCAGTAATGTAt                                           >  1:130027/1‑100 (MQ=255)
                                                     gcACATCGTACACCAGATGCAACATGCTTTTTTGCGTCGGTTATTCGAGCGGGCCATGCAGCGTAATGCGATCAAACTCTGGCGCAGGCAGTAATg                                              <  2:1533604/96‑1 (MQ=255)
                                                     gcACATCATACACCAAATGCACCATCCTTTTCAGCGTCGGTTGTTCGAGCGGGCCATGCAGCGTAATGCGACCAAACTCTGGCGCAGGCAGTAATGTATc                                          <  1:852708/100‑1 (MQ=255)
                                                         atcatACACCAAATGCACCAGCCTTTTCAGCGTCGGTTGTTCGAGCGGGCCATGCAGCGTAATGCGACCAAACTCTGGCGCAGGCAGTAATGTATCCAgc                                      <  1:716888/100‑1 (MQ=255)
                                                            atACACCAAATGCACCAGCCTTTTCAGCGTCGGTTGTTCGAGCGGGCCATGCAGCGTAATGCGACCAAACTCTGGCGCAGGCAGTAATGTATCCAGCGCa                                   >  1:2015947/1‑100 (MQ=255)
                                                             tACACCAAATGCACCAGCCTTTTCAGCGTCGGTTGTTCGAGCGGGCAATGCAGCGTAATGCGACCAAACTCTGGCGCAGGCAGTAATGTATCCAGCGCaa                                  >  2:1423200/1‑100 (MQ=255)
                                                              acacCAAATGCACCAGCCTTTTCAGCGTCGGTTGTTCGAGCGGGCCATGCAGCGTAATGCGACCAAACTCTGGCGCAGGCAGTAATGTATCCAGCGCAAc                                 >  2:96893/1‑100 (MQ=255)
                                                               cacCAAATGCACCAGCCTTTTCAGCGTCGGTTGTTCGAGCGGGCCATGCAGCGTAATGCGACCAAACTCTGGCGCAGGCAGTAATGTATCCAGCGCAACg                                >  2:729323/1‑100 (MQ=255)
                                                               cacCAAATGCACCAGCCTTTTCAGCGTCGGTTGTACGAGCGGGCCATGCAGCGTAATGCGACCAAACTCTGGCGCAGGCAGTAATGTATCCAGCGCAACg                                >  1:1260548/1‑100 (MQ=255)
                                                                  cAAATGCACCAGCCTTTTCAGCGTCGGTTGTTCGAGCGGGCCATGCAGCGTAATGCGACCAATCTCTGGCGCAGGCAGTAATGTATCCAGCGCAACGTGc                             <  2:1364939/100‑1 (MQ=255)
                                                                  cAAATGCACCAGCCTTTTCAGCGTCGGTTGTTCGAGCGGGCCATGCAGCGTAATGCGACCAAACTCTGGCGCAGGCAGTAATGTATCCAGCGCAACGTGc                             <  2:611266/100‑1 (MQ=255)
                                                                   aaaTGCACCAGCCTTTTCAGCGTCGGTTGTTCGAGCGGGCCATGCAGCGTAATGCGACCAAACTCTGGCGCAGGCAGTAATGTATCCAGCGCAACGTGc                             <  2:1671546/99‑1 (MQ=255)
                                                                    aaTGCACCCGCCTTTTCAGCGTCGGTTGTTCGAACGGGCCATCCAGCGTAATGCGACCAAACTCTGCCGCACGCAGTAATGTATCCTCCCCAACTTGc                             >  2:2332638/1‑98 (MQ=255)
                                                                    aaTGCACCAGCCTTTTCAGCGTCGGTTGTTCGAGCGGGCCATGCAGCGTAATGCGACCAAACTCTGGCGCAGGCAGTAATGTATCCAGCGCAACGTGCTg                           >  2:2518088/1‑100 (MQ=255)
                                                                     aTGCACCAGCCTTTTCAGCGTCGGTTGTTCGAGCGGGCCATGCAGCGTAATGCGACCAAACTCTGGCGCAGGCAGTAATGTATCCAGCGCAACGTGCTg                           <  2:377849/99‑1 (MQ=255)
                                                                     aTGCACCAGCCTTTTCAGCGTCGGTTGTTCGAGCGGGCCATGCAGCGTAATGCGACCAAACTCTGGCGCAGGCAGTAATGTATCCAGCGCAACGTGCTGt                          <  1:1126893/100‑1 (MQ=255)
                                                                      tGCACCAGCCTTTTCAGCGTCGGTTGTTCGAGCGGGCCATGCAGCGTAATGCGACCAAACTCTGGCGCAGGCAGTAATGTATCCAGCGCAACGTGCTg                           <  1:1093462/98‑1 (MQ=255)
                                                                      tGCACCAGCCTTTTCAGCGTCGGTTGTTCGAGCGGGCCATGCAGCGTAATGCGACCAAACTCTGGCGCAGGCAGTAATGTATCCAGCGCAACGTGCTGtt                         >  1:917828/1‑100 (MQ=255)
                                                                       gCACCAGCCTTTTCAGCGTCGGTTGTTCGAGCGGGCCATGCAGCGTAATGCGACCAAACTCTGGCGCAGGCAGTAATGTATCCAGCGCAACGTGCTGTTc                        <  1:1398496/100‑1 (MQ=255)
                                                                        cACCAGCCTTTTCAGCGTCGGTTGTTCGAGCGGGCCATGCAGCGTAATGCGACCAAACTCTGGCGCAGGCAGTAATGTATCCAGCGCAACGTGCTGTTCa                       <  2:130025/100‑1 (MQ=255)
                                                                        cACCAGCCTTTTCAGCGTCGGTTGTTCGAGCGGGCCATGCAGCGTAATGCGACCAAACTCTGGCGCAGGCAGTAATGTATCCAGCGCAACGTGCTGTTCa                       >  2:1070586/1‑100 (MQ=255)
                                                                           cAGCCTTTTCAGCGTCGGTTGTTCGAGCGGGCCATGCAGCGTAATGCGACCAAACTCTGGCGCAGGCAGTAATGTATCCAGCGCAACGTGCTGTTCATGc                    <  1:791450/100‑1 (MQ=255)
                                                                           cAGCCTTTTCAGCGTCGGTTGTTCGAGCGGGCCATGCAGCGTAATGCGACCAAACTCTGGCGCAGGCAGTAATGTATCCAGCGCAACGTGCTGTTCATGc                    <  1:2494787/100‑1 (MQ=255)
                                                                            aGCCTTTTCAGCGTCGGTTGTTCGAGCGGGCCATGCAGCGTAATGCGACCAAACTCTGGCGCAGGCAGTAATGTATCCAGCGCAACGTGCTGTTCATGcc                   <  1:2110033/100‑1 (MQ=255)
                                                                            aGCCTTTTCAGCGTCGGTTGTTCGAGCGGGCCATGCAGCGTAATGCGACCAAACTCTGGCGCAGGCAGTAATGTATCCAGCGCAACGTGCTGTTCATGcc                   >  2:2487838/1‑100 (MQ=255)
                                                                             gCCTTTTCAGCGTCGGTTGTTCGAGCGGGCCATGCAGCGTAATGCGACCAAACTCTGGCGCAGGCAGTAATGTATCCAGCGCAACGTGCTGTTCATGccc                  <  1:1459834/100‑1 (MQ=255)
                                                                             gCCTTTTCAGCGTCGGTTGTTCGAGCGGGCCATGCAGCGTAATGCGACCAAACTCTGGCGCAGGCAGTAATGTATCCAGCGCAACGTGCTGTTCATGccc                  <  2:270320/100‑1 (MQ=255)
                                                                             gCCTTTTCAGCGTCGGTTGTTCGAGCGGGCCATGCAGCGTAATGCGACCAAACTCTGGCGCAGGCAGTAATGTATCCAGCGCAACGTGCTGTTCATGccc                  >  1:802593/1‑100 (MQ=255)
                                                                              ccTTTTCAGCGTCGGTTGTTCGAGCGGGCCATGCAGCGTAATGCGACCAAACTCTGGCGCAGGCAGTAATGTATCCAGCGCAACGTGCTGTTCATGCCCa                 <  1:62335/100‑1 (MQ=255)
                                                                               cTTTTCAGCGTCGGTTGTTCGAGCGGGCCATGCAGCGTAATGCGATCAAACTCTGGCGCAGGCAGTAATGTATCCAGCGCAACGTGCTGTTCATGCCCaa                <  2:1927532/100‑1 (MQ=255)
                                                                               cTTTTCAGCGTCGGTTGTTCGAGCGGGCCATGCAGCGTAATGCGACCAAACTCTGGCGCAGGCAGTAATGTATCCAGCGCAACGTGCTGTTCATGCCCaa                <  2:1633287/100‑1 (MQ=255)
                                                                                ttttCAGCGTCGGTTGTTCGAGCGGGCCATGCAGCGTAATGCGACCAAACTCTGGCGCAGGCAGTAATGTATCCAGCGCAACGTGCTGTTCATGCCCAAt               <  1:1992694/100‑1 (MQ=255)
                                                                                  ttCAGGGTCGGTTGTTCGTGCGGGCGATGCGGCGTAATGCGACCAGAGCCTGGCGCAGGGAGTAATGTATCCAGCGCAACGTGCTGTTCa                       <  2:1721900/90‑1 (MQ=255)
                                                                                  ttCAGCGTCGGTTGTTCGAGCGGGCCATGCAGCGTAATGCGACCAAACTCTGGCGCAGGCAGTAATGTATCCAGCGCAACGTGCTGTTCATGCCCAATaa             >  1:895629/1‑100 (MQ=255)
                                                                                  ttCAGCGTCGGTTGTTCGAGCGGGCCATGCAGCGTAATGCGACCAAACTCTGGCGCAGGCAGTAATGTATCCAGCGCAACGTGCGGTTCATGCCCAATaa             >  1:2521142/1‑100 (MQ=255)
                                                                                     aGCGTCGGTTGATCGAGCGGGCCATGCAGCGTAATGCGACCAAACTCTGGCGCAGGCAGTAATGTATCCAGCGCAACGTGCTGTTCATGCCCAATAAACt          >  2:366610/1‑100 (MQ=255)
                                                                                     aGCGTCGGATGTTCGAGCGGGCCATGCAGCGTAATGCGACCAAACTCTGGCGCAGGCAGTAATGTATCCAACGGACCGTGCTGTGGATGCCCAATAAACt          >  2:1669699/1‑100 (MQ=255)
                                                                                      gCGTCGGTTGTTCGAGCGGGCCATGCAGCGTAATGCGACCAAACTCTGGCGCAGGCAGTAATGTATCCAGCGCAACGTGCTGTTCATGCCCAATAAAc           <  2:2225656/98‑1 (MQ=255)
                                                                                      gCGTCGGTTGTTCGAGCGGGCCATGCAGCGTAATGCGACCAAACTCTGGCGCAGGCAGTAATGTATCCAGCGCAACGTGCTGTTCATGCCCAATAAACtt         <  2:1803793/100‑1 (MQ=255)
                                                                                      gCGTCGGTTGTTCGAGCGGGCCATGCAGCGTAATGCGACCAAACTCTGGCGCAGGCAGTAATGTATCCAGCGCAACGTGCTGTTCATGCCCAATAAACtt         >  1:1828406/1‑100 (MQ=255)
                                                                                       cGTCGGTTGTTCGAGCGGGCCATGCAGCGTAATGCGACCAAACTCTGGCGCAGGCAGTAATGTATCCAGCGCAACGTGCTGTTCATGCCCAATAAACtt         <  1:1765225/99‑1 (MQ=255)
                                                                                       cGTCGGTTGATCGAGCGGGCCATGCAGCGTAATGCGACCAAACTCTGGCGCAGGCAGTAATGTATCCAGCGCAACGTGCTGTTCATGCCCAATAAACTTg        >  2:1518181/1‑100 (MQ=255)
                                                                                          cGGTTGTTCGAGCGGGCCATGCAGCGTAATGCGACCAAACTCTGGCGCAGGCAGTAATGTATCCAGCGCAACGTGCTGTTCATGCCCAATAAACTTGCta     <  1:1001248/100‑1 (MQ=255)
                                                                                          cGGTTGTTCGAGCGGGCCATGCAGCGTAATGCGACCAAACTCTGGCGCAGGCAGTAATGTATCCAGCGCAACGTGCTGTTCATGCCCAATAAACTTGCta     <  2:1828392/100‑1 (MQ=255)
                                                                                           ggTTGTTCGAGCGGGCCATGCAGCGTAATGCGACCAAACTCTGGCGCAGGCAGTAATGTATCCAGCGCAACGTGCTGTTCATGCCCAATAAACTTGCtat    <  2:2015451/100‑1 (MQ=255)
                                                                                           ggTTGTTCGAGCGGGCCATGCAGCGTAATGCGACCAAACTCTGGCGCAGGCAGTAATGTATCCAGCGCAACGTGCTGTTCATGCCCAATAAACTTGCtat    >  2:908722/1‑100 (MQ=255)
                                                                                            gttgttCGAGCGGGCCATGCAGCGTAATGCGACCAAACTCTGGCGCAGGCAGTAATGTATCCAGCGCAACGTGCTGTTCATGCCCAATAAACTTGCtata   <  1:2487853/100‑1 (MQ=255)
                                                                                             ttGTTCGAGCGGGCCATGCAGCGTAATGCGACCAAACTCTGGCGCAGGCAGTAATGTATCCAGCGCAACGTGCTGTTCATGCCCAATAAACTTGCTATaa  >  2:1816365/1‑100 (MQ=255)
                                                                                                |                                                                                                
ATCGAACTCACCCGGTATCCCGGCGACTTTACGCAGCGGTGCGTCATCGCGGCGCACATCATACACCAAATGCACCAGCCTTTTCAGCGTCGGTTGATCGAGCGGGCCATGCAGCGTAATGCGACCAAACTCTGGCGCAGGCAGTAATGTATCCAGCGCAACGTGCTGTTCATGCCCAATAAACTTGCTATAA  >  NC_000913/2436841‑2437033

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A