Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A12 F204 I0 R1	247	174.3	8556966	94.2%	8060661	99.8

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_000913	2,436,937	A→T	100%	D305E (GAT→GAA)	pdxB ←	erythronate‑4‑phosphate dehydrogenase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,436,937	0	A	T	98.4%	628.7 / ‑0.6	183	D305E (GAT→GAA)	pdxB	erythronate‑4‑phosphate dehydrogenase
Reads supporting (aligned to +/- strand): ref base A (1/2); new base T (66/114); total (67/116)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.23e-01

Only 100 of 184 total aligned reads displayed.

TATCGAACTCACCCGGTATCCCGGCGACTTTACGCAGCGGTGCGTCATCGCGGCGCACATCATACACCAAATGCACCAGCCTTTTCAGCGTCGGTTGATCGAGCGGGCCATGCAGCGTAATGCGACCAAACTCTGGCGCAGGCAGTAATGTATCCAGCGCAACGTGCTGTTCATGCCCAATAAACTTGCTATA  >  NC_000913/2436840‑2437032

 aTCGAACTCACCCGGTATCCCGGCGACTTTACGCAGCGGTGCGTCATCGCGGCGCACATCATACACCAAATGCACCAGCCTTTTCAGCGTCGGTTGTTCg                                                                                              <  1:933402/100‑1 (MQ=255)

 aTCGAACTCACCCGGTATCCCGGCGACTTTACGCAGCGGTGCGTCATCGCGGCGCACATCATACACCAAATGCACCAGCCTTTTCAGCGTCGGTTGTTCg                                                                                              <  1:4074087/100‑1 (MQ=255)

  tCGAACTCACCCGGTATCCCGGCGACTTTACGCAGCGGTGCGTCATCGCGGCGCACATCATACACCAAATGCACCAGCCTTTTCAGCGTCGGTTGTTCGa                                                                                             >  2:2535966/1‑100 (MQ=255)

   cGAACTCACCCGGTATCCCGGCGACTTTACGCAGCGGTGCGTCATCGCGGCGCACATCATACACCAAATGCACCAGCCTTTTCAGCGTCGGTTGTTCGAg                                                                                            <  1:2313519/100‑1 (MQ=255)

    gAACTCACCCGGTATCCCGGCGACTTTACGCAGCGGTGCGTCATCGCGGCGCACATCATACACCAAATGCACCAGCCTTTTCAGCGTCGGTTGTTCGAGc                                                                                           >  1:2534406/1‑100 (MQ=255)

       cTCACCCGGTATCCCGGCGACTTTACGCAGCGGTGCGTCATCGCGGCGCACATCATACACCAAATGCACCAGCCTTTTCAGCGTCGGTTGTTCGAGCggg                                                                                        >  2:3489315/1‑100 (MQ=255)

        tCACCCGGTATCCCGGCGACTTTACGCAGCGGTGCGTCATCGCGGCGCACATCATACACCAAATGCACCAGCCTTTTCAGCGTCGGTTGTTCGAGCGGGc                                                                                       <  1:840182/100‑1 (MQ=255)

        tCACCCGGTATCCCGGCGACTTTACGCAGCGGTGCGTCATCGCGGCGCACATCATACACCAAATGCACCAGCCTTTTCAGCGTCGGTTGTTCGAGCGGGc                                                                                       >  2:3443918/1‑100 (MQ=255)

        tCACCCGGTATCCCGGCGACTTTACGCAGCGGTGCGTCATCGCGGCGCACATCATACACCAAATGCACCAGCCTTTTCAGCGTCGGTTGTTCGAGCGGGc                                                                                       >  1:1771584/1‑100 (MQ=255)

         cACCCGGTATCCCGGCGACTTTACGCAGCGGTGCGTCATCGCGGCGCACATCATACACCAAATGCACCAGCCTTTTCAGCGTCGGTTGTTCGAGCGGGcc                                                                                      >  2:2244354/1‑100 (MQ=255)

         cACCCGGTATCCCGGCGACTTTACGCAGCGGTGCGTCATCGCGGCGCACATCATACACCAAATGCACCAGCCTTTTCAGCGTCGGTTGTTCGAGCGGGcc                                                                                      >  1:301863/1‑100 (MQ=255)

         cACCCGGTATCCCGGCGACTTTACGCAGCGGTGCGTCATCGCGGCGCACATCATACACCAAATGCACCAGCCTTTTCAGCGTCGGTTGTTCGAGCGGGcc                                                                                      <  1:399061/100‑1 (MQ=255)

           cccGGTATCCCGGCGACTTTACGCAGCGGTGCGTCATCGCGGCGCACATCATACACCAAATGCACCAGCCTTTTCAGCGTCGGTTGTTCGAGCGGGCCAt                                                                                    <  2:677374/100‑1 (MQ=255)

            ccGGTATCCCGGCGACTTTACTCAGCGGTGCGTCATCGCGGCGCCCATCATACACCAAATGCACCAGCCTTTTCAGCGTCGGTTGTTCGAGCGGGc                                                                                       <  1:2116555/96‑1 (MQ=255)

            ccGGTATCCCGGCGACTTTACGCAGCGGTGCGTCATCGCGGCGCACATCATACACCAAATGCACCAGCCTTTTCAGCGTCGGTTGTTCGAGCGGGCCATg                                                                                   <  2:4146689/100‑1 (MQ=255)

            ccGGTATCCCGGCGACTTTACGCAGCGGTGCGTCATCGCGGCGCACATCATACACCAAATGCACCAGCCTTTTCAGCGTCGGTTGTTCGAGCGGGCCATg                                                                                   >  1:3800695/1‑100 (MQ=255)

             cGGTATCCCGGCGACTTTACGCAGCGGTGCGTCATCGCGGCGCACATCATACACCAAATGCACCAGCCTTTTCAGCGTCGGTTGTTCGAGCGGGCCATGc                                                                                  >  2:3552803/1‑100 (MQ=255)

              ggTATCCCGGCGACTTTACGCAGCGGTGCGTCATCGCGGCGCACATCATACACCAAATGCACCAGCCTTTTCAGCGTCGGTTGTTCGAGCGGGCCATGCa                                                                                 <  2:1266340/100‑1 (MQ=255)

              ggTATCCCGGCGACTTTACGCAGCGGTGCGTCATCGCGGCGCACATCATACACCAAATGCACCAGCCTTTTCAGCGTCGGTTGTTCGAGCGGGCCATGCa                                                                                 <  1:4160943/100‑1 (MQ=255)

               gTATCCCGGCGACTTTACGCAGCGGTGCGTCATCGCGGCGCACATCATACACCAAATGCACCAGCCTTTTCAGCGTCGGTTGTTCGAGCGGGCCATGCAg                                                                                <  2:1832126/100‑1 (MQ=255)

                 aTCCCGGCGACTTTACGCAGCGGTGCGTCATCGCGGCGCACATCATACACCAAATGCACCAGCCTTTTCAGCGTCGGTTGTTCGAGCGGGCCATGCAGCg                                                                              <  1:3367881/100‑1 (MQ=255)

                  tCCCGGCGACTTTACGCAGCGGTGCGTCATCGCGGCGCACATCATACACCAAATGCTCCAGCCTTTTCAGCGTCGGTTGTTCGTGCGGGCCATGCAGCGt                                                                             <  2:239026/100‑1 (MQ=255)

                   cccGGCGACTTTACGCAGCGGTGCGTCATCGCGGCGCACATCATACACCAAATGCACCAGCCTTTTCAGCGTCGGTTGTTCGAGCGGGCCATGCAGCGt                                                                             <  1:392067/99‑1 (MQ=255)

                    ccGGCGACTTTACGCAGCGGTGCGTCATCGCGGCGCACATCATACACCAAATGCACCAGCCTTTTCAGCGTCGGTTGTTCGAGCGGGCCATGCAGCGTaa                                                                           >  2:1100019/1‑100 (MQ=255)

                    ccGGCGACTTTACGCAGCGGTGCGTCATCGCGGCGCACATCATACACCAAATGCACCAGCCTTTTCAGCGTCGGTTGATCGAGCGGGCCATGCAGCGTaa                                                                           >  2:2948934/1‑100 (MQ=255)

                      ggCGACTTTCCGCAGCGGTGCGTCATCGCGGCGCACATCATACACCAAATGCACCAGCCTTTTCAGCGTCGGTTGTTCGAGCGGGCCATGCAGCGTAATg                                                                         <  1:3146691/100‑1 (MQ=255)

                      ggCGACTTTACGCAGCGGTGCGTCATCGCGGCGCACATCATACACCAAATGCACCAGCCTTTTCAGCGTCGGTTGTTCGAGCGGGCCATGCAGCGTAATg                                                                         <  2:4122952/100‑1 (MQ=255)

                      ggCGACTTTACGCAGCGGTGCGTCATCGCGGCGCACATCATACACCAAATGCACCAGCCTTTTCAGCGTCGGTTGTTCGAGCGGGCCATGCAGCGTAATg                                                                         <  1:3435311/100‑1 (MQ=255)

                       gCGACTTTACGCAGCGGTGCGTCATCGCGGCGCACATCATACACCAAATGCACCAGCCTTTTCAGCGTCGGTTGTTCGAGCGGGCCATGCAGCGTAATGc                                                                        <  1:1003012/100‑1 (MQ=255)

                        cGACTTTACGCAGCGGTGCGTCATCGCGGCGCACATCATACACCAAATGCACCAGCCTTTTCAGCGTCGGTTGTTCGAGCGGGCCATGCAGCGTAATGCg                                                                       <  2:2468633/100‑1 (MQ=255)

                          aCTTTACGCAGCGGTGCGTCATCGCGGCGCACATCATACACCAAATGCACCAGCCTTTTCAGCGTCGGTTGTTCGAGCGGGCCATGCAGCGTAATGCGAc                                                                     <  2:479328/100‑1 (MQ=255)

                           cTTTACGCAGCGGTGCGTCATCGCGGCGCACATCATACACCAAATGCACCAGCCTTTTCAGCGTCGGTTGTTCGAGCGGGCCATGCAGCGTAATGCGa                                                                      >  2:2914189/1‑98 (MQ=255)

                           cTTTACGCAGCGGTGCGTCATCGCGGCGCACATCATACACCAAATGCACCAGCCTTTTCAGCGTCGGTTGTTCGAGCGGGCCATGCAGCGTAATGCGAcc                                                                    <  2:2336681/100‑1 (MQ=255)

                           cTTTACGCAGCGGTGCGTCATCGCGGAGCACATCATACACCAAATGCACCAGCCTTTTCAGCGTACATTGTTAGAGAGGACCATGCAGCGTAATGCGa                                                                      >  1:388631/1‑98 (MQ=255)

                               aCGCAGCGGTGCGTCATCGCGGCGCACATCATACACCAAATGCACCAGCCTTTTCAGCGTCGGTTGTTCGAGCGGGCCATGCAGCGTAATGCGACCAAAc                                                                <  1:3587237/100‑1 (MQ=255)

                                cacAGCGGTGCGTCATCGCGGCGCACATCATACACCAAATGCACCAGCCTTTTCAGCGTCGGTTGTTCGAGCGGGCCATGCAGCGTAATGCGACCAAAct                                                               >  2:2218669/3‑100 (MQ=255)

                                    gCGGTGCGTCATCGCGGCGCACATCATACACCAAATGCACCAGCCTTTTCAGCGTCGGTTGTTCGAGCGGGCCATGCAGCGTAATGCGACCAAACTCTgg                                                           <  1:1816493/100‑1 (MQ=255)

                                    gCGGTGCGCCATCGCGGCGCACATCATACACCAAATGCGCCAGCCTTTTCAGCGTCGGTTGTTCGAGCGGGCCATGCAGCGTAATGCGACCAAACTCTgg                                                           <  1:1017849/100‑1 (MQ=255)

                                     cGGTGCGTCATCGCGGCGCACATCATACACCAAATGCACCAGCCTTTTCAGCGTCGGTTGTTCGAGCGGGCCATGCAGCGTAATGCGACCAAACTCTGgc                                                          <  1:3100916/100‑1 (MQ=255)

                                      ggTGCGTCATCGCGGCGCACATCATACACCAAATGCACCAGCCTTTTCAGCGTCGGTTGTTCGAGCGGGCCATGCAGCGTAATGCGACCAAACTCTGgcg                                                         <  2:3041421/100‑1 (MQ=255)

                                      ggTGCGTCATCGCGGCGCACATCATACACCAAATGCACCAGCCTTTTCAGCGTCGGTTGTTCGAGCGGGCCATGCAGCGTAATGCGACCAAACTCTGgcg                                                         <  2:728509/100‑1 (MQ=255)

                                      ggTGCGTCATCGCGGCGCACATCATACACCAAATGCACCAGCCTTTTCAGCGTCGGTTGTTCGAGCGGGCCATGCAGCGTAATGCGACCAAACTCTGgcg                                                         <  1:749811/100‑1 (MQ=255)

                                        tGCGTCATCGCGGCGCACATCATACACCAAATGCACCAGCCTTTTCAGCGTCGGTTGTTCGAGCGGGCCATGCAGCGTAATGCGACCAAACTCTGGCgca                                                       <  2:3684998/100‑1 (MQ=255)

                                         gCGTCATCGCGGCGCACATCATACACCAAATGCACCAGCCTTTTCAGCGTCGGTTGTTCGAGCGGGCCATGCAGCGTAATGCGACCAAACTCTGGCgcag                                                      <  1:440550/100‑1 (MQ=255)

                                         gCGTCATCGCGGCGCACATCATACACCAAATGCACCAGCCTTTTCAGCGTCGGTTGTTCGAGCGGGCCATGCAGCGTAATGCGACCAAACTCTGGCgcag                                                      <  1:144657/100‑1 (MQ=255)

                                         gCGTCATCGCGGCGCACATCATACACCAAATGCACCAGCCTTTTCAGCGTCGGTTGTTCGAGCGGGCCAGGCAGCGTAATGCGACCAAACTCTGgcgc                                                        <  1:1473524/98‑1 (MQ=255)

                                          cGTCATCGCGGCGCACATCATACACCAAATGCACCAGCCTTTTCAGCGTCGGTTGTTCGAGCGGGCCATGCAGCGTAATGCGACCAAACTCTGGCgcagg                                                     >  2:1344641/1‑100 (MQ=255)

                                          cGTCATCGCGGCGCACATCATACACCAAATGCACCAGCCTTTTCAGCGTCGGTTGTTCGAGCGGGCCATGCAGCGTAATGCGACCAAACTCTGGCgcagg                                                     <  2:3185836/100‑1 (MQ=255)

                                           gTCCTCACGGCGCACATCATACACCAAATGCACCAGCCTCTTCCCCGTCGGGTGTACGAGCGTGCTATGCAGCGTAATGCGTCCCAACTCTGGCgccggc                                                    >  1:2102257/1‑100 (MQ=255)

                                           gTCATCGCGGCGCACATCATACACCAAATGCACCAGCCTTTTCAGCGTCGGTTGTTCGAGCGGGCCATGCAGCGTAATGCGACCAAACTCTGGCgcaggc                                                    <  2:883631/100‑1 (MQ=255)

                                           gTCATCGCGGCGCACATCATACACCAAATGCACCAGCCTTTTCAGCGTCGGTTGTTCGAGCGGGCCATGCAGCGTAATGCGACCAAACTCTGGCgcaggc                                                    <  1:1867430/100‑1 (MQ=255)

                                           gTCATCGCGGCGCACATCATACACCAAATGCACCAGCCTTTTCAGCGTCGGTTGTTCGAGCGGGCCATGCAGCGTAATGCGACCAAACTCTGGCgcaggc                                                    <  1:4015756/100‑1 (MQ=255)

                                              aTCGCGGCGCACATCATACACCAAATGCACCAGCCTTTTCAGCGTCGGTTGTTCGAGCGGGCCATGCAGCGTAATGCGACCAAACTCTGGCGCAGGCAGt                                                 >  2:3321692/1‑100 (MQ=255)

                                              aTCGCGGCGCACATCATACACCAAATGCACCAGCCTTTTCAGCGTCGGTTGTTCGAGCGGGCCATGCAGCGTAATGCGACCAAACTCTGGCGCAGGCAGt                                                 <  2:334764/100‑1 (MQ=255)

                                                 gcggcgCACATCATACACCAAATGCACCAGCCTTTTTAGCGTCGGTTGTTCGAGCGGGCCATGCAGCGTAATGCGACCAAACTCTGGCGCAGGCAGTAAt                                              <  2:3550117/100‑1 (MQ=255)

                                                    gcgcACATCATACACCAAATGCACCAGCCTTTTCAGCGTCGGTTGTTCGAGCGGGCCATGCAGCGTAATGCGACCAAACTCTGGCGCAGGCAGTAATGTa                                           <  2:1891319/100‑1 (MQ=255)

                                                     cgcACATCATACACCAAATGCACCAGCCTTTTCAGCGTCGGTTGTTCGAGCGGGCCATGCAGCGTAATGCGACCAAACTCTGGCGCAGGCAGTAATGTAt                                          <  2:2530983/100‑1 (MQ=255)

                                                       cacaTCATACACCAAATGCACCAGCCTTTTCAGCGTCGGTTGTTCGAGCGGGCCATGCAGCGTAATGCGACCAAACTCTGGCGCAGGCAGTAATGTATcc                                        <  1:1935310/100‑1 (MQ=255)

                                                       cacaTCATACACCAAATGCACCAGCCTTTTCAGCGTCGGTTGTTCGAGCGGGCCATGCAGCGTAATGCGACCAAACTCTGGCGCAGGCAGTAATGTATcc                                        <  1:3249729/100‑1 (MQ=255)

                                                        acaTCATACACCAAATGCACCAGCCTTTTCAGCGTCGGTTGTTCGAGCGGGCCATGCAGCGTAATGCGACCAAACTCTGGCGCAGGCAGTAATGTATCCa                                       <  1:2424155/100‑1 (MQ=255)

                                                         catcatACACCAAATGCACCAGCCTTTTCAGCGTCGGTTGTTCGAGCGGGCCATGCAGCGTAATGCGACCAAACTCTGGCGCAGGCAGTAATGTATCCAg                                      >  2:2281004/1‑100 (MQ=255)

                                                         catcatACACCAAATGCACCAGCCTTTTCAGCGTCGGTTGTTCGAGCGGGCCATGCAGCGTAATGCGACCAAACTCTGGCGCAGGCAGTAATGTATCCAg                                      <  1:345517/100‑1 (MQ=255)

                                                           tcatACACCAAATGCACCAGCCTTTTCAGCGTCGGTTGTTCGAGCGGGCCATGCAGCGTAATGCGACCAAACTCTGGCGCAGGCAGTAATGTATCCAgcg                                    >  2:2831718/1‑100 (MQ=255)

                                                           tcatACACCAAATGCACCAGCCTTTTCAGCGTCGGTTGATCGAGCGGGCCATGCTGCGTAATGCGACCAAACTCTGGCGCAGGCAGTAATGTATCCAgcg                                    <  2:1542973/100‑1 (MQ=255)

                                                            catACACCAAATGCACCAGCCTTTTCAGCGTCGGTTGTTCGAGCGGGCCATGCAGCGTAATGCGACCAAACTCTGGCGCAGGCAGTAATGTATCCAgcgc                                   <  2:3629843/100‑1 (MQ=255)

                                                            catACACCAAATGCACCAGCCTTTTCAGCGTCGGTTGTTCGAGCGGGCCATGCAGCGTAATGCGACCAAACTCTGGCGCAGGCAGTAATGTATCCAgcgc                                   <  2:202215/100‑1 (MQ=255)

                                                             atACACCAAATGCACCAGCCTTTTCAGCGTCGGTTGTTCGAGCGGGCCATGCAGCGTAATGCGACCAAACTCTGGCGCAGGCAGTAATGTATCCAGCGCa                                  <  2:2714841/100‑1 (MQ=255)

                                                              tACACCAAATGCACCAGCCTTTTCAGCGTCGGTTGTTCGAGCGGGCCATGCAGCGTAATGCGACCAAACTCTGGCGCAGGCAGTAATGTATCCAGCGCaa                                 >  2:2853556/1‑100 (MQ=255)

                                                              tACACCAAATGCACCAGCCTTTTCAGCGTCGGTTGTTCGAGCGGGCCATGCAGCGTAACGCGAACAAACTCTGGCGCAGGCAGTAATGTATCCAGCGCaa                                 >  1:1049071/1‑100 (MQ=255)

                                                                cacCAAATGCACCAGCCTTTTCAGCGTCGGTTGTTCGAGCGGGCCATGCAGCGTAATGCGACCAAACTCTGGCGCAGGCAGTAATGTATCCAGCGCAACg                               <  2:1689965/100‑1 (MQ=255)

                                                                 acCAAATGCACCAGCCTTTTCAGCGTCGGTTGTTCGAGCGGGCCATGCAGCGTAATGCGACCAAACTCTGGCGCAGGCAGTAATGTATCCAGCGCAACGt                              >  2:4152616/1‑100 (MQ=255)

                                                                 acCAAATGCACCAGCCTTTTCAGCGTCGGTTGTTCGAGCGGGCCATGCAGCGTAATGCGACCAAACTCTGGCGCAGGCAGTAATGTATCCAGCGCAACGt                              >  1:3734251/1‑100 (MQ=255)

                                                                 acCAAATGCACCAGCCTTTTCAGCGTCGGTTGTTCGAGCGGGCCATGCAGCGTAATGCGACCAAACTCTGGCGCAGGCAGTAATGTATCCAGCGCAACGt                              >  2:3106629/1‑100 (MQ=255)

                                                                  cctaaTGCTCCAGCCTTTTCAGCGTCGGTTGTTCGAGCGTGCCATGCAGCGTAATGCGACCAAACTCTGGCGCAGGCAGTAATGTATCCAGCGCAACg                               <  1:2395693/95‑1 (MQ=255)

                                                                   cgatctcACCAGCCTTTTCAGCGTCGGTTGTTCGAGCGGGCCATGCAGCGTAATGCGACCAAACTCTGGCGCAGGCAGTAATGTATCCAGCGCAACGTGc                            <  1:3959667/94‑1 (MQ=255)

                                                                   cAAATGCACCAGCCTTTTCAGCGTCGGTTGTTCGAGCGGGCCATGCAGCGTAATGCGACCAAACTCTGGCGCAGGCAGTAATGTATCCAGCGCAACGTGc                            <  1:2004709/100‑1 (MQ=255)

                                                                   cAAATGCACCAGCCTTTTCAGCGTCGGTTGTTCGAGCGGGCCATGCAGCGTAATGCGACCAAACTCTGGCGCAGGCAGTAATGTATCCAGCGCAACGTGc                            <  2:130890/100‑1 (MQ=255)

                                                                        gCACCAGCCTGTTCAGCGTCGGTTGTTCGAGCGGGCCATGCAGCGTAATGCGACCAAACTCTGGCGAAGGCAGTAATGTATCCAGCGCAACGTGCTGTTc                       <  1:2815300/100‑1 (MQ=255)

                                                                         cACCAGCCTTTTCAGCGTCGGTTGTTCGAGCGGGCCATGCAGCGTAATGCGACCAAACTCTGGCGCTGGCAGTAATGTATCCAGCGCAACGTGCTGTTCa                      <  1:2805775/100‑1 (MQ=255)

                                                                         cACCAGCCTTTTCAGCGTCGGTTGTTCGAGCGGGCCATGCAGCGTAATGCGACCAAACTCTGGCGCAGGCAGTAATGTATCCAGCGCAACGTGcagatcg                      >  2:3959687/1‑94 (MQ=255)

                                                                         cACCAGCCTTTTCAGCGTCGGTTGTTCGAGCGGGCCATGCAGCGTAATGCGACCAAACTCTGGCGCAGGCAGTAATGTATCCAGCGCAACGTGCTGTTCa                      >  2:3282756/1‑100 (MQ=255)

                                                                         cACCAGCCTTTTCAGCGTCGGTTGTTCGAGCGGGCCATGCAGCGTAATGCGACCAAACTCTGGCGCAGGCAGTAATGTATCCAGCGCAACGTGCTGTTCa                      >  1:386521/1‑100 (MQ=255)

                                                                         cACCAGCCTTTTCAGCGTCGGTTGTTCGAGCGGGCCATGCAGCGTAATGCGACCAAACTCTGGCGCAGGCAGTAATGTATCCAGCGCAACGTGCTGTTCa                      <  1:3524204/100‑1 (MQ=255)

                                                                           ccAGCCTTTTCAGCGTCGGTTGTTCGAGCGGGCCATGCAGCGTAATGCGACCAAACTCTGGCGCAGGCAGTAATGTATCCAGCGCAACGTGCTGTTCa                      >  1:275847/1‑98 (MQ=255)

                                                                           ccAGCCTTTTCAGCGTCGGTTGTTCGAGCGGGCCATGCAGCGTAATGCGACCAAACTCTGGCGCAGGCAGTAATGTATCCAGCGCAACGTGCTGTTCATg                    >  1:772505/1‑100 (MQ=255)

                                                                            cAGCCTTTTCAGCGTCGGTTGTTCGAGCGGGCCATGCAGCGTAATGCGACCAAACTCTGGCGCAGGCAGTAATGTATCCAGCGCAACGTGCTGTTCATGc                   <  1:3995289/100‑1 (MQ=255)

                                                                              gCCTTTTCAGCGTCGGTTGTTCGAGCGGGCCATGCAGCGTAATGCGACCAAACTCTGGCGCAGGCAGTAATGTATCCAGTGCAACGTGCTGTTCATGccc                 <  1:3461960/100‑1 (MQ=255)

                                                                              gCCTTTTCAGCGTCGGTTGTTCGAGCGGGCCATGCAGCGTAATGCGACCAAACTCTGGCGCAGGCAGTAATGTATCCAGCGCAACGTGCTGTTCATGccc                 >  2:2572880/1‑100 (MQ=255)

                                                                                    tCAGCGTCGGTTGTTCGAGCGGGCCATGCAGCGTAATGCGACCAAACTCTGGCGCAGGCAGTAATGTATCCAGCGCAACGTGCTGTTCATGCCCAATaaa           <  2:3880643/100‑1 (MQ=255)

                                                                                     cAGCGTCGGTTGTTCGAGCGGGCCATGCAGCGTAATGCGACCAAACTCTGGCGCAGGCAGTAATGTATCCAGCGCAACGTGCTGTTCATGCCCAATAAAc          >  1:1157628/1‑100 (MQ=255)

                                                                                     cAGCGTCGGTTGTTCGAGCGGGCCATGCAGCGTAATGCGACCAAACTCTGGCGCAGGCAGTAATGTATCCAGCGCAACGTGCTGTTCATGCCCAATAAAc          <  1:2158234/100‑1 (MQ=255)

                                                                                      aGCGTCGGTTGTTCGAGCGTGCCATGCAGCGTAATGCGACCAAACTCTGGCGCAGGTAGTAATGTATCCAGCGCAACGTGCTCTTCATGCCGAATAAACt         <  1:3308900/100‑1 (MQ=255)

                                                                                      aGCGTCGGTTGTTCGAGCGGGCCATGCAGCGTAATGCGACCAAACTCTGGCGCAGGCAGTAATGTATCCAGCGCAACGTGCTGTTCATGCCCAATAAACt         <  2:3252487/100‑1 (MQ=255)

                                                                                       gCGTCGGTTGTTCGAGCGGGCCATGCAGCGTAAGGCGACCAAACTCTGGCGCAGGCAGTAATGTATCCAGAGCAACGTGCTGTTCATGCCCAATAAACtt        >  1:2570281/1‑100 (MQ=255)

                                                                                        cGTCGGTTGTTCGAGCGGGCCATGCAGCGTAAGGCGACCAAACTCTGGCGCAGGCAGTAATGTATCCAGCGCAACGTGCTGTTCATGCCCAATAAACTTg       <  2:2610980/100‑1 (MQ=255)

                                                                                         gTCGGTTGTTCGAGCGGGCCATGCAGCGTAATGCGACCAAACTCTGGCGCAGGCAGTAATGTATCCAGCGCAACGTGCTGTTCATGCCCAATAAACTTGc      <  1:286318/100‑1 (MQ=255)

                                                                                          tCGGTTGTTCGAGCGGGCCATGCAGCGTAATGCGACCAAACTCTGGCGCAGGCAGTAATGTATCCAGCGCAACGTGCTGTTCATGCCCAATAAACTTGCt     <  1:637754/100‑1 (MQ=255)

                                                                                          tCGGTTGTTCGAGCGGGCCATGCAGCGTAATGCGACCAAACTCTGGCGCAGACAGTAATGTATCCAGCGCAACGTGCTGTTCATGCCCAATAAACTTGCt     <  1:1765387/100‑1 (MQ=255)

                                                                                           cGGTTGTTCGAGCGGGCCATGCAGCGTAATGCGACCAAACTCTGGCGCAGGCAGTAATGTATCCAGCGCAACGTGCTGTTCATGCCCAATAAACTTGCta    <  2:3222091/100‑1 (MQ=255)

                                                                                             gttgttCGAGCGGGCCATGCAGCGTAATGCGACCAAACTCTGGCGCAGGCAGTAATGTATCCAGCGCAACGTGCTGTTCATGCCCAATAAACTTGCtata  <  1:692559/100‑1 (MQ=255)

TATCGAACTCACCCGGTATCCCGGCGACTTTACGCAGCGGTGCGTCATCGCGGCGCACATCATACACCAAATGCACCAGCCTTTTCAGCGTCGGTTGATCGAGCGGGCCATGCAGCGTAATGCGACCAAACTCTGGCGCAGGCAGTAATGTATCCAGCGCAACGTGCTGTTCATGCCCAATAAACTTGCTATA  >  NC_000913/2436840‑2437032

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 12 ≤` `ATCG/ATCG` `< 32 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A