Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A11 F212 I1 R1 174 80.0 3892211 96.7% 3763768 99.9

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 2,436,937 A→T D305E (GAT→GAA)  pdxB ← erythronate‑4‑phosphate dehydrogenase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,436,9370AT100.0% 215.4 / NA 61D305E (GAT→GAA) pdxBerythronate‑4‑phosphate dehydrogenase
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base T (26/35);  total (26/35)

TATCGAACTCACCCGGTATCCCGGCGACTTTACGCAGCGGTGCGTCATCGCGGCGCACATCATACACCAAATGCACCAGCCTTTTCAGCGTCGGTTGATCGAGCGGGCCATGCAGCGTAATGCGACCAAACTCTGGCGCAGGCAGTAATGTATCCAGCGCAACGTGCTGTTCATGCCCAATAAACTTGCTAT  >  NC_000913/2436840‑2437031
                                                                                                 |                                                                                              
tATCGAACTCACCCGGTATCCCGGCGACTTTACGCAGCGGTGCGTCATCGCGGCGCACATCATACACCAAATGCACCAGCCTTTTCAGCGTCGGTTGTTc                                                                                              <  2:1709498/100‑1 (MQ=255)
 aTCGAACTCACCCGGTATCCCGGCGACTTTACGCAGCGGTGCGTCATCGCGGCGCACATCATACACCAAATGCACCAGCCTTTTCAGCGTCGGTTGTTCg                                                                                             <  1:725086/100‑1 (MQ=255)
 aTCGAACTCACCCGGTATCCCGGCGACTTTACGCAGCGGTGCGTCATCGCGGCGCACATCATACACCAAATGCACCAGCCTTTTCAGCGTCGGTTGTTCg                                                                                             <  1:807178/100‑1 (MQ=255)
   cGAACTCACCCGGTATCCCGGCGACTTTACGCAGCGGTTCGTCATCGCGGCGCACATCATACACCAAATGCACCAGCCTTTTCAGCGTCGGTTGTTCGAg                                                                                           >  2:1667992/1‑100 (MQ=255)
   cGAACTCACCCGGTATCCCGGCGACTTTACGCAGCGGTGCGTCATCGCGGCGCACATCATACACCAAATGCACCAGCCTTTTCAGCGTCGGTTGTTCGAg                                                                                           >  1:819222/1‑100 (MQ=255)
         cACCCGGTATCCCGGCGACTTTACGCAGCGGTGCGTCATCGCGGCGCACATCATACACCAAATGCACCAGCCTTTTCAGCGTCGGTTGTTCGAGCGGGcc                                                                                     >  1:1094061/1‑100 (MQ=255)
          aCCCGGTATCCCGGCGACTTTACGCAGCGGTGCGTCATCGCGGCGCACATCATACACCAAATGCACCAGCCTTTTCAGCGTCGGTTGTTCGAGCGGGCCa                                                                                    <  1:504556/100‑1 (MQ=255)
           cccGGTATCCCGGCGACTTTACGCAGCGGTGCGTCATCGCGGCGCACATCATACACCAAATGCACCAGCCTTTTCAGCGTCGGTTGTTCGAGCGGGCCAt                                                                                   <  2:552633/100‑1 (MQ=255)
                   cccGGCGACTTTACGCAGCGGTGCGTCATCGCGGCGCACATCATACACCAAATGCACCAGCCTTTTCAGCGTCGGTTGTTCGAGCGGGCCATGCAGCGTa                                                                           >  2:1343539/1‑100 (MQ=255)
                    ccGGCGACTTTACGCAGCGGTGCGTCATCGCGGCGCACATCATACACCAAATGCACCAGCCTTTTCAGCGTCGGTTGTTCGAGCGGGCCATGCAGCGTaa                                                                          >  2:1106838/1‑100 (MQ=255)
                    ccGGCGACTTTACGCAGCGGTGCGTCATCGCGGCGCACATCATACACCAAATGCACCAGCCTTTTCAGCGTCGGTTGTTCGAGCGGGCCATGCAGCGTaa                                                                          >  1:1393757/1‑100 (MQ=255)
                       gCGACTTTACGCAGCGGTGCGTCATCGCGGCGCACATCATACACCAAATGCACCAGCCTTTTCAGCGTCGGTTGTTCGAGCGGGCCATGCAGCGTAATGc                                                                       <  1:841717/100‑1 (MQ=255)
                        cGACTTTACGCAGCGGTGCGTCATCGCGGCGCACATCATACACCAAATGCACCAGCCTTTTCAGCGTCGGTTGTTCGAGCGGGCCATGCAGCGTAATGCg                                                                      <  1:302143/100‑1 (MQ=255)
                          aCTTTACGCAGCGGTGCGTCATCGCGGCGCACATCATACACCAAATGCACCAGCCTTTTCAGCGTCGGTTGTTCGAGCGGGCCATGCAGCGTAATGCGAc                                                                    <  2:1228754/100‑1 (MQ=255)
                          aCTTTACGCAGCGGTGCGTCATCGCGGCGCACATCATACACCAAATGCACCAGCCTTTTCAGCGTCGGTTGTTCGAGCGGGCCATGCAGCGTAATGCGAc                                                                    <  2:1479148/100‑1 (MQ=255)
                            tttACGCAGCGGTGCGTCATCGCGGCGCACATCATACACCAAATGCACCAGCCTTTTCAGCGTCGGTTGTTCGAGCGGGCCATGCAGCGTAATGCGACCa                                                                  <  1:938250/100‑1 (MQ=255)
                            tttACGCAGCGGTGCGTCATCGCGGCGCACATCATACACCAAATGCACCAGCCTTTTCAGCGTCGGTTGTTCGAGCGGGCCATGCAGCGTAATGCGACCa                                                                  <  1:996432/100‑1 (MQ=255)
                                cGCAGCGGTGCGTCATCGCGGCGCACATCATACACCAAATGCACCAGCCTTTTCAGCGTCGGTTGTTCGAGCGGGCCATGCAGCGTAATGCGACCAAAct                                                              >  2:1737824/1‑100 (MQ=255)
                                 gCAGCGGTGCGTCATCGCGGCGCACATCATACACCAAATGCACCAGCCTTTTCAGCGTCGGTTGTTCGAGCGGGCCATGCAGCGTAATGCGACCAAActc                                                             <  1:1087542/100‑1 (MQ=255)
                                   aGCGGTGCGTCATCGCGGCGCACATCATACACCAAATGCACCAGCCTTTTCAGCGTCGGTTGTTCGAGCGGGCCATGCAGCGTAATGCGACCAAACTCTg                                                           >  2:1508215/1‑100 (MQ=255)
                                   aGCGGTGCGTCATCGCGGCGCACATCATACACCAAATGCACCAGCCTTTTCAGCGTCGGTTGTTCGAGCGGGCCATGCAGCGTAATGCGACCAAACTCTg                                                           <  1:412034/100‑1 (MQ=255)
                                         gCGTCATCGCGGCGCACATCATACACCAAATGCACCAGCCTTTTCAGCGTCGGTTGTTCGAGCGGGCCATGCAGCGTAATGCGACCAAACTCTGGCgcag                                                     <  1:984379/100‑1 (MQ=255)
                                           gTCATCGCGGCGCACATCATACACCAAATGCACCAGCCTTTTCAGCGTCGGTTGTTCGAGCGGGCCATGCAGCGTAATGCGACCAAACTCTGGCgcaggc                                                   <  2:1393765/100‑1 (MQ=255)
                                           gTCATCGCGGCGCACATCATACACCAAATGCACCAGCCTTTTCAGCGTCGGTTGTTCGAGCGGGCCATGCAGCGTAATGCGACCAAACTCTGGCgcaggc                                                   <  1:1872405/100‑1 (MQ=255)
                                             cATCGCGGCGCACATCATACACCAAATGCACCAGCCTTTTCAGCGTCGGTTGTTCGAGCGGGCCATGCAGCGTAATGCGACCAAACTCTGGCgcaggcag                                                 <  1:1136182/100‑1 (MQ=255)
                                             cATCGCGGCGCACATCATACACCAAATGCACCAGCCTTTTCAGCGTCGGTTGTTCGAGCGGGCCATGCAGCGTAATGCGACCAAACTCTGGCgcaggcag                                                 >  1:261817/1‑100 (MQ=255)
                                              aTCGCGGCGCACATCATACACCAAATGCACCAGCCTTTTCAGCGTCGGTTGTTCGAGCGGGCCATGCAGCGTAATGCGACCAAACTCTGGCGCAGGCAGt                                                <  2:1672532/100‑1 (MQ=255)
                                                   ggcgCACATCATACACCAAATGCACCAGCCTTTTCAGCGTCGGTTGTTCGAGCGGGCCATGCAGCGTAATGCGACCAAACTCTGGCGCAGGCAGTAATGt                                           >  1:1705052/1‑100 (MQ=255)
                                                          ntcatACACCAAATGCACCAGCCTTTTCAGCGTCGGTTGTTCGAGCGGGCCATGCAGCGTAATGCGACCAAACTCTGGCGCTGGCAGTAATGTATCCAgc                                    >  1:806717/2‑100 (MQ=255)
                                                           tcatACACCAAATGCACCAGCCTTTTCAGCGTCGGTTGTTCGAGCGGGCCATGCAGCGTAATGCGACCAAACTCTGGCGCAGGCAGTAATGTATCCAgcg                                   >  2:1298665/1‑100 (MQ=255)
                                                            catACACCAAATGCACCAGCCTTTTCAGCGTCGGTTGTTCGAGCGGGCCATGCAGCGTAATGCGACCAAACTCTGGCGCAGGCAGTAATGTATCCAgcgc                                  <  2:1274801/100‑1 (MQ=255)
                                                             atACACCAAATGCACCAGCCTTTTCAGCGTCGGTTGTTCGAGCGGGCCATGCAGCGTAATGCGACCAAACTCTGGCGCAGGCAGTAATGTATCCAGCGCa                                 >  1:1781146/1‑100 (MQ=255)
                                                               acacCAAATGCACCAGCCTTTTCAGCGTCGGTTGTTCGAGCGGGCCATGCAGCGTAATGCGACCAAACTCTGGCGCAGGCAGTAATGTATCCAGCGCAAc                               <  1:324196/100‑1 (MQ=255)
                                                               acacCAAATGCACCAGCCTTTTCAGCGTCGGTTGTTCGAGCGGGCCATGCAGCGTAATGCGACCAAACTCTGGCGCAGGCAGTAATGTATCCAGCGCAAc                               <  1:755091/100‑1 (MQ=255)
                                                                cacCAAATGCACCAGCCTTTTCAGCGTCGGTTGTTCGAGCGGGCCATGCAGCGTAATGCGACCAAACTCTGGCGCAGGCAGTAATGTATCCAGCGCAACg                              >  2:531164/1‑100 (MQ=255)
                                                                cacCAAATGCACCAGCCTTTTCAGCGTCGGTTGTTCGAGCGGGCCATGCAGCGTAATGCGACCAAACTCTGGCGCAGGCAGTAATGTATCCAGCGCAACg                              >  1:910840/1‑100 (MQ=255)
                                                                  ccAAATGCACCAGCCTTTTCAGCGTCGGTTGTTCGAGCGGGCCATGCAGCGTAATGCGACCAAACTCTGGCGCAGGCAGTAATGTATCCAGCGCAACGTg                            >  2:76918/1‑100 (MQ=255)
                                                                   cAAATGCACCAGCCTTTTCAGCGTCGGTTGTTCGAGCGGGCCATGCAGCGTAATGCGACCAAACTCTGGCGCAGGCAGTAATGTATCCAGCGCAACGTGc                           <  1:1106837/100‑1 (MQ=255)
                                                                   cAAATGCACCAGCCTTTTCAGCGTCGGTTGTTCGAGCGGGCCATGCAGCGTAATGCGACCAAACTCTGGCGCAGGCAGTAATGTATCCAGCGCAACGTGc                           >  1:86836/1‑100 (MQ=255)
                                                                   cAAATGCACCAGCCTTTTCAGCGTCGGTTGTTCGAGCGGGCCATGCAGCGTAATGCGACCAAACTCTGGCGCAGGCAGTAATGTATCCAGCGCAACGTGc                           <  1:1667982/100‑1 (MQ=255)
                                                                     aaTGCACCAGCCTTTTCAGCGTCGGTTGTTCGAGCGGGCCATGCAGCGTAATGCGACCAAACTCTGGCGCAGGCAGTAATGTATCCAGCGCAACGTGCTg                         <  1:302085/100‑1 (MQ=255)
                                                                       tGCACCAGCCTTTTCAGCGTCGGTTGTTCGAGCGGGCCATGCAGCGTAATGCGACCAAACTCTGGCGCAGGCAGTAATGTATCCAGCGCAACGTGCTGtt                       >  2:237907/1‑100 (MQ=255)
                                                                        gCACCAGCCTTTTCAGCGTCGGTTGTTCGAGCGGGCCATGCAGCGTAATGCGACCAAACTCTGGCGCAGGCAGTAATGTATCCAGCGCAACGTGCTGTTc                      >  2:1882738/1‑100 (MQ=255)
                                                                        gCACCAGCCTTTTCAGCGTCGGTTGTTCGAGCGGGCCATGCAGCGTAATGCGACCAAACTCTGGCGCAGGCAGTAATGTATCCAGCGCAACGTGCTGTTc                      >  1:34589/1‑100 (MQ=255)
                                                                        gCACCAGCCTTTTCAGCGTCGGTTGTTCGAGCGGGCCATGCAGCGTAATGCGACCAAACTCTGGCGCAGGCAGTAATGTATCCAGCGCAACGTGCTGTTc                      >  1:625665/1‑100 (MQ=255)
                                                                         cACCAGCCTTTTCAGCGTCGGTTGTTCGAGCGGGCCTTGCAGCGTAATGCGACCAAACTCTGGCGCAGGCAGTAATGTATCCAGCGCAACGTGCTGTTCa                     >  2:1371625/1‑100 (MQ=255)
                                                                         cACCAGCCTTTTCAGCGTCGGTTGTTCGAGCGGGCCATGCAGCGTAATGCGACCAAACTCTGGCGCAGGCAGTAATGTATCCAGCGCAACGTGCTGTTCa                     <  2:1797907/100‑1 (MQ=255)
                                                                          aCCAGCCTTTTCAGCGTCGGTTGTTCGAGCGGGCCATGCAGCGTAATGCGACCAAACTCTGGCGCAGGCAGTAATGTATCCAGCGCAACGTGCTGTTCAt                    <  2:1122099/100‑1 (MQ=255)
                                                                             aGCCTTTTCAGCGTCGGTTGTTCGAGCGGGCCATGCAGCGTAATGCGACCAAACTCTGGCGCAGGCAGTAATGTATCCAGCGCAACGTGCTGTTCATGcc                 <  2:162854/100‑1 (MQ=255)
                                                                             aGCCTTTTCAGCGTCGGTTGTTCGAGCGGGCCATGCAGCGTAATGCGACCAAACTCTGGCGCAGGCAGTAATGTATCCAGCGCAACGTGCTGTTCATGcc                 <  2:819223/100‑1 (MQ=255)
                                                                             aGCCTTTTCAGCGTCGGTTGTTCGAGCGGGCCATGCAGCGTAATGCGACCAAACTCAGGCGCAGGCAGTAATGTATCCAGCGCAACGTGCTGTTCATGcc                 <  2:1631583/100‑1 (MQ=255)
                                                                               ccTTTTCAGCGTCGGTTGTTCGAGCGGGCCATGCAGCGTAATGCGACCAAACTCTGGCGCAGGCAGTAATGTATCCAGCGCAACGTGCTGTTCATGccc                <  2:1334525/99‑1 (MQ=255)
                                                                               ccTTTTCAGCGTCGGTTGTTCGAGCGGGCCATGCAGCGTAATGCGACCAAACTCTGGCGCAGGCAGTAATGTATCCAGCGCAACGTGCTGTTCATGCCCa               >  2:1157628/1‑100 (MQ=255)
                                                                                  tttCAGCGTCGGTTGTTCGAGCGGGCCATGCAGCGTAATGCGACCAAACTCTGGCGCAGGCAGTAATGTATCCAGCGCAACGTGCTGTTCATGCCCAATa            <  2:267232/100‑1 (MQ=255)
                                                                                    tCAGCGTCGGTTGTTCGAGCGGGCCATGCAGCGTAATGCGACCAAACTCTGGCGCAGGCAGTAATGTATCCAGCGCAACGTGCTGTTCATGCCCAATaaa          <  2:625666/100‑1 (MQ=255)
                                                                                         gTCGGTTGTTCGAGCGGGCCATGCAGCGTAATGCGACCAAACTCTGGCGCAGGCAGTAATGTATCCAGCGCAACGTGCTGTTCATGCCCAATAAACTTGc     <  2:261819/100‑1 (MQ=255)
                                                                                         gTCGGTTGTTCGAGCGGGCCATGAAGAGTAATGCGACCAAACACTGGCGCAGGCAGTAATGTATCCAGCGCAACGTGCTGTTCATGCCCAATAAACtt       <  1:673347/98‑1 (MQ=255)
                                                                                           cGGTTGTTCGAGCGGGCCATGCAGCGTAATGCGACCAAACTCTGGCGCAGGCAGTAATGTATCCAGCGCAACGTGCTGTTCATGCCCAATAAACTTGCta   >  1:132436/1‑100 (MQ=255)
                                                                                           cGGTTGTTCGAGCGGGCCATGCAGCGTAATGCGACCAAACTCTGGCGCAGGCAGTAATGTATCCAGCGCAACGTGCTGTTCATGCCCAATAAACTTGCta   >  1:1424546/1‑100 (MQ=255)
                                                                                           cGGTTGTTCGAGCGGGCCATGCAGCGTAATGCGACCAAACTCTGGCGCAGGCAGTAATGTATCCAGCGCAACGTGCTGTTCATGCCCAATAAACTTGCta   <  2:408151/100‑1 (MQ=255)
                                                                                            ggTTGTTCGAGCGGGCCATGCAGCGTAATGCGACCAAACTCTGGCGCAGGCAGTAATGTATCCAGCGCAACGTGCTGTTCATGCCCAATAAACTTGCtat  >  2:1870209/1‑100 (MQ=255)
                                                                                                 |                                                                                              
TATCGAACTCACCCGGTATCCCGGCGACTTTACGCAGCGGTGCGTCATCGCGGCGCACATCATACACCAAATGCACCAGCCTTTTCAGCGTCGGTTGATCGAGCGGGCCATGCAGCGTAATGCGACCAAACTCTGGCGCAGGCAGTAATGTATCCAGCGCAACGTGCTGTTCATGCCCAATAAACTTGCTAT  >  NC_000913/2436840‑2437031

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A