Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A11 F212 I1 R1 174 80.0 3892211 96.7% 3763768 99.9

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
JC NC_000913 4,242,942 Δ66 bp coding (510‑575/891 nt) malG ← maltose transporter subunit

New junction evidence
  seq id position reads (cov) reads (cov) score skew freq annotation gene product
* ? NC_000913 = 42429412 (0.020)56 (0.750) 42/184 1.1 96.7% coding (576/891 nt) malG maltose transporter subunit
?NC_000913 4243008 = 2 (0.030)coding (509/891 nt) malG maltose transporter subunit

AATGGCAGCGATAAACGACAGGATGAATACCACCGCCAGAATCGGTACTGACAGCGGCAACAGGACAAGGCGGAAGGCCTGCCACGGTGTCGCACCATCCAGCGCA‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑  >  NC_000913/4242836‑4242941
‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑cagcgcaATCCCACCCAGATACGCGAAAATTACGCCGCCGTGAGTATTCAGGCCAATGAATGGAATGTACTCACCCAGACGATCAAACAACGCATACAACGCGA  >  NC_000913/4243008‑4243104
                                                                                                                                                                                                           
AATGGCAGCGATAAACGACAGGATGAATACCACCGCCAGAATCGGTACTGACAGCGGCAACAGGACAAGGCGGAAGGCCTGCCACGGTGTCGCACCATCC                                                                                                         >  1:528781/1‑100
 ATGGCAGCGATAAACGACAGGATGAATACCACCGCCAGAATCGGTACTGACAGCGGCAACAGGACAAGGCGGAAGGCCTGCCACGGTGTCGCACCATCCA                                                                                                        >  1:1753388/1‑100
   GGCAGCGATAAACGACAGGATGAATACCACCGCCAGAATCGGTACTGACAGCGGCAACAGGACAAGGCGGAAGGCCTGCCACGGTGTCGCACCATCCAGC                                                                                                      >  2:252730/1‑100
      AGCGATAAACGACAGGATGAATACCACCGCCAGAATCGGTACTGACAGCGGCAACAGGACAAGTCGGAAGGCCTGCCACGGTGTCGCACCATCCAGCGCA                                                                                                   <  2:1603359/100‑1
         GATAAACGACAGGATGAATACCACCGCCAGAATCGGTACTGACAGCGGCAACAGGACAAGGCGGAAGGCCTGCCACGGTGTCGCACCATCCAGCGCAATC                                                                                                >  1:1157916/1‑100
         GATAAACGACAGGATGAATACCACCGCCAGAATCGGTACTGACAGCGGCAACAGGACAAGGCGGAAGGCCTGCCACGGTGTCGCACCATCCAGCGCAATC                                                                                                >  1:967099/1‑100
          ATAAACGACAGGATGAATACCACCGCCAGAATCGGTACTGACAGCGGCAACAGGACAAGGCGGAAGGCCTGCCACGGTGTCGCACCATCCAGCGCAATCC                                                                                               <  2:1352714/100‑1
            AAACGACAGGATGAATACCACCGCCAGAATCGGTACTGACAGCGGCAACAGGACAAGGCGGAAGGCCTGCCACGGTGTCGCACCATCCAGCGCAATCCCA                                                                                             <  2:335436/100‑1
              ACGACAGGATGAATACCACCGCCAGAATCGGTACTGACAGCGGCAACAGGACAAGGCGGAAGGCCTGCCACGGTGTCGCACCATCCAGCGCAATCCCAC                                                                                            <  1:1833492/99‑1
                GACAGGATGAATACCACCGCCAGAATCGGTACTGACAGCGGCAACAGGACAAGGCGGAAGGCCTGCCACGGTGTCGCACCATCCAGCGCAATCCCACCCA                                                                                         <  2:162555/100‑1
                    GGATGAATACCACCGCCAGAATCGGTACTGACAGCGGCAACAGGACAAGGCGGAAGGCCTGCCACGGTGTCGCACCATCCAGCGCAATCCCACCCAGATA                                                                                     >  1:1487726/1‑100
                     GATGAATACCACCGCCAGAATCGGTACTGACAGCGGCAACAGGACAAGGCGGAAGGCCTGCCACGGTGTCGCACCATCCAGCGCAATCCCACCCAGATAC                                                                                    >  2:313379/1‑100
                       TGAATACCACCGCCAGAATCGGTACTGACAGCGGCAACAGGACAAGGCGGAAGGCCTGCCACGGTGTCGCACCATCCAGCGCAATCCCACCCAGATACGC                                                                                  >  2:172553/1‑100
                         AATACCACCGCCAGAATCGGTACTGACAGCGGCAACAGGACAAGGCGGAAGGCCTGCCACGGTGTCGCACCATCCAGCGCAATCCCACCCAGATACGCGA                                                                                >  2:586585/1‑100
                            ACCACCGCCAGAATCGGTACTGACAGCGGCAACAGGACAAGGCGGAAGGCCTGCCACGGTGTCGCACCATCCAGCGCAATCCCACCCAGATACGCGAAAA                                                                             >  2:1590185/1‑100
                            ACCACCGCCAGAATCGGTACTGACAGCGGCAACAGGACAAGGCGGAAGGCCTGCCACGGTGTCGCACCATCCAGCGCAATCCCACCCAGATACGCGAAAA                                                                             <  2:170943/100‑1
                              CACCGCCAGAATCGGTACTGACAGCGGCAACAGGACAAGGCGGAAGGCCTGCCACGGTGTCGCACCATCCAGCGCAATCCCACCCAGATACGCGAAAATT                                                                           <  1:1010426/100‑1
                                CCGCCAGAATCGGTACTGACAGCGGCAACAGGACAAGGCGGAAGGCCTGCCACGGTGTCGCACCATCCAGCGCAATCCCACCCAGATACGCGAAAATTAC                                                                         >  1:1903048/1‑100
                                   CCAGAATCGGTACTGACAGCGGCAACAGGACAAGGCGGAAGGCCTGCCACGGTGTCGCACCATCCAGCGCAATCCCACCCAGATACGCGAAAATTACGCC                                                                      >  2:1668189/1‑100
                                           GGTACTGACAGCGGCAACAGGACAAGGCGGAAGGCCTGCCACGGTGTCGCACCATCCAGCGCAATCCCACCCAGATACGCGAAAATTACGCCGCCGTGAG                                                              <  2:131533/100‑1
                                             TACTGACAGCGGCAACAGGACAAGGCGGAAGGCCTGCCACGGTGTCGCACCATCCAGCGCAATCCCACCCAGATACGCGAAAATTACGCCGCCGTGAGTA                                                            <  1:1520692/100‑1
                                              ACTGACAGCGGCAACAGGACAAGGCGGAAGGCCTGCCACGGTGTCGCACCATCCAGCGCAATCCCACCCAGATACGCGAAAATTACGCCGCCGTGAGTAT                                                           <  2:846947/100‑1
                                               CTGACAGCGGCAACAGGACAAGGCGGAAGGCCTGCCACGGTGTCGCACCATCCAGCGCAATCCCACCCAGATACGCGAAAATTACGCCGCCGTGcg                                                              >  1:890079/1‑94
                                                TGACAGCGGCAACAGGACAAGGCGGAAGGCCTGCCACGGCGTCGCACCATCCAGCGCAATCCCACCCAGATACGCGAAAATTACGCCGCCGTGAGTATTC                                                         <  2:140299/100‑1
                                                  ACAGCGGCAACAGGACAAGGCGGAAGGCCTGCCACGGTGTCGCACCATCCAGCGCAATCCCACCCAGATACGCGAAAATTACGCCGCCGTGAGTATTCAG                                                       >  2:502411/1‑100
                                                   CAGCGGCAACAGGACAAGGCGGAAGGCCTGCCACGGTGTCGCACCATCCAGCGCAATCCCACCCAGATACGCGAAAATTACGCCGCCGTGAGTATTCAGG                                                      >  2:206572/1‑100
                                                     GCGGCAACAGGACAAGGCGGAAGGCCTGCCACGGTGTCGCACCATCCAGCGCAATCCCACCCAGATACGCGAAAATTACGCCGCCGTGAGTATTCAGGCC                                                    <  2:1775192/100‑1
                                                         CAACAGGACAAGGCGGAAGGCCTGCCACGGTGTCGCACCATCCAGCGCAATCCCACCCAGATACGCGAAAATTACGCCGCCGTGAGTATTCAGGCCAATG                                                >  2:1541137/1‑100
                                                         CAACAGGACAAGGCGGAAGGCCTGCCACGGTGTCGCACCATCCAGCGCAATCCCACCCAGATACGCGAAAATTACGCCGCCGTGAGTATTCAGGCCAATG                                                >  2:1012251/1‑100
                                                           ACAGGACAAGGCGGAAGGCCTGCCACGGTGTCGCACCATCCAGCGCAATCCCACCCAGATACGCGAAAATTACGCCGCCGTGAGTATTCAGGCCAATGAA                                              >  2:1548676/1‑100
                                                            CAGGACAAGGCGGAAGGCCTGCCACGGTGTCGCACCATCCAGCGCAATCCCACCCAGATACGCGAAAATTACGCCGCCGTGAGTATTCAGGCCAATGAAT                                             >  1:1460525/1‑100
                                                            CAGGACAAGGCGGAAGGCCTGCCACGGTGTCGCACCATCCAGCGCAATCCCACCCAGATACGCGAAAATTACGCCGCCGTGAGTATTCAGGCCAATGAA                                              >  1:1078923/1‑99
                                                             AGGACAAGGCGGAAGGCCTGCCACGGTGTCGCACCATCCAGCGCAATCCCACCCAGATACGCGAAAATTACGCCGCCGTGAGTATTCAGGCCAATGAATG                                            <  1:362741/100‑1
                                                             AGGACAAGGCGGAAGGCCTGCCACGGTGTCGCACCATCCAGCGCAATCCCACCCAGATACGCGAAAATTACGCCGCCGTGAGTATTCAGGCCAATGAATG                                            <  1:1763602/100‑1
                                                              GGACAAGGCGGAAGGCCTGCCACGGTGTCGCACCATCCAGCGCAATCCCACCCAGATACGCGAAAATTACGCCGCCGTGAGTATTCAGGCCAATGAATG                                            <  2:1276620/99‑1
                                                                ACAAGGCGGAAGGCCTGCCACGGTGTCGCACCATCCAGCGCAATCCCACCCAGATACGCGAAAATTACGCCGCCGTGAGTATTCAGGCCAATGAATGGAA                                         <  2:1903061/100‑1
                                                                 CAAGGCGGAAGGCCTGCCACGGTGTCGCACCATCCAGCGCAATCCCACCCAGATACGCGAAAATTACGCCGCCGTGAGTATTCAGGCCAATGAATGGAAT                                        >  2:252214/1‑100
                                                                 CAAGGCGGAAGGCCTGCCACGGTGTCGCACCATCCAGCGCAATCCCACCCAGATACGCGAAAATTACGCCGCCGTGAGTATTCAGGCCAATGAATGGAAT                                        <  2:1305457/100‑1
                                                                 CAAGGCGGAAGGCCTGCCACGGTGTCGCACCATCCAGCGCAATCCCACCCAGATACGCGAAAATTACGCCGCCGTGAGTATTCAGGCCAATGAATGGAAT                                        >  2:453887/1‑100
                                                                 CAAGGCGGAAGGCCTGCCACGGTGTCGCACCATCCAGCGCAATCCCACCCAGATACGCGAAAATTACGCCGCCGTGAGTATTCAGGCCAATGAATGGAAT                                        >  2:875381/1‑100
                                                                 CAAGGCGGAAGGCCTGCCACGGTGTCGCACCATCCAGCGCAATCCCACCCAGATACGCGAAAATTACGCCGCCGTGAGTATTCAGGCCAATGAATGGAAT                                        >  2:1062899/1‑100
                                                                   AGGCGGAAGGCCTGCCACGGTGTCGCACCATCCAGCGCAATCCCACCCAGATACGCGAAAATTACGCCGCCGTGAGTATTCAGGCCAATGAATGGAATGT                                      >  1:999604/1‑100
                                                                     GCGGAAGGCCTGCCACGGTGTCGCACCATCCAGCGCAATCCCACCCAGATACGCGAAAATTACGCCGCCGTGAGTATTCAGGCCAATGAATGGAATGTAC                                    <  2:1227519/100‑1
                                                                      CGGAAGGCCTGCCACGGTGTCGCACCATCCAGCGCAATCCCACCCAGATACGCGAAAATTACGCCGCCGTGAGTATTCAGGCCAATGAATGGAATGTACT                                   >  2:704654/1‑100
                                                                      CGGAAGGCCTGCCACGGTGTCGCACCATCCAGCGCAATCCCACCCAGATACGCGAAAATTACGCCGCCGTGAGTATTCAGGCCAATGAATGGAATGTACT                                   <  2:1024719/100‑1
                                                                      CGGAAGGCCTGCCACGGTGTCGCACCATCCAGCGCAATCCCACCCAGATACGCGAAAATTACGCCGCCGTGAGTATTCAGGCCAATGAATGGAATGTACT                                   <  1:586583/100‑1
                                                                      CGGAAGGCCTGCCACGGTGTCGCACCATCCAGCGCAATCCCACCCAGATACGCGAAAATTACGCCGCCGTGAGTATTCAGGCCAATGAATGGAATGTACT                                   >  1:583047/1‑100
                                                                             CCTGCCACGGTGTCGCACCATCCAGCGCAATCCCACCCAGATACGCGAAAATTACGCCGCCGTGAGTATTCAGGCCAATGAATGGAATGTACTCACCCAG                            >  2:1590737/1‑100
                                                                             CCTGCCACGGTGTCGCACCATCCAGCGCAATCCCACCCAGATACGCGAAAATTACGCCGCCGTGAGTATTCAGGCCAATGAATGGAATGTACTCACCCAG                            >  2:1591730/1‑100
                                                                                GCCACGGTGTCGCACCATCCAGCGCAATCCCACCCAGATACGCGAAAATTACGCCGCCGTGAGTATTCAGGCCAATGAATGGAATGTACTCACCCAGACG                         <  1:1631474/100‑1
                                                                                  CACGGTGTCGCACCATCCAGCGCAATCCCACCCAGATTCGCGAAAATTACGCCGCCGTGAGTATTCAGGCCAATGAATGGAATGTACTCACCCAGACG                         <  1:29041/98‑1
                                                                                  CACGGTGTCGCACCATCCAGCGCAATCCCACCCAGATACGCGAAAATTACGCCGCCGTGAGTATTCAGGCCAATGAATGGAATGTACTCACCCAGACGAT                       <  1:1692813/100‑1
                                                                                  CACGGTGTCGCACCATCCAGCGCAATCCCACCCAGATACGCGAAAATTACGCCGCCGTGAGTATTCAGGCCAATGAATGGAATGTACTCACCCAGACGAT                       <  1:172552/100‑1
                                                                                       TGTCGCACCATCCAGCGCAATCCCACCCAGATACGCGAAAATTACGCCGCCGTGAGTATTCAGGCCAATGAATGGAATGTACTCACCCAGACGATCAAAC                  <  1:1012250/100‑1
                                                                                       TGTCGCACCATCCAGCGCAATCCCACCCAGATACGCGAAAATTACGCCGCCGTGAGTATTCAGGCCAATGAATGGAATGTACTCACCCAGACGATCAAAC                  >  2:1440996/1‑100
                                                                                           GCACCATCCAGCGCAATCCCACCCAGATACGCGAAAATTACGCCGCCGTGAGTATTCAGGCCAATGAATGGAATGTACTCACCCAGACGATCAAACAACG              <  2:1401348/100‑1
                                                                                            CACCATCCAGCGCAATCCCACCCAGATACGCGAAAATTACGCCGCCGTGAGTATTCAGGCCAATGAATGGAATGTACTCACCCAGACGATCAAACAACGC             <  1:876019/100‑1
                                                                                            CACCATCCAGCGCAATCCCACCCAGATACGCGAAAATTACGCCGCCGTGAGTATTCAGGCCAATGAATGGAATGTACTCACCCAGACGATCAAACAACGC             <  1:1673106/100‑1
                                                                                             ACCATCCAGCGCAATCCCACCCAGATACGCGAAAATTACGCCGCCGTGAGTATTCAGGCCAATGAATGGAATGTACTCACCCAGACGATCAAACAACGCA            <  1:252728/100‑1
                                                                                                ATCCAGCGCAATCCCACCCAGATACGCGAAAATTACGCCGCCGTGAGTATTCAGGCCAATGAATGGAATGTACTCACCCAGACGATCAAACAACGCATAC         <  2:585421/100‑1
                                                                                                    AGCGCAATCCCACCCAGATACGCGAAAATTACGCCGCCGTGAGTATTCAGGCCAATGAATGGAATGTACTCACCCAGACGATCAAACAACGCATACAACG     <  2:521074/100‑1
                                                                                                     GCGCAATCCCACCCAGATACGCGAAAATTACGCCGCCGTGAGTATTCAGGCCAATGAATGGAATGTACTCACCCAGACGATCAAACAACGCATACAACGC    <  2:577119/100‑1
                                                                                                      CGCAATCCCACCCAGATACGCGAAAATTACGCCGCCGTGAGTATTCAGGCCAATGAATGGAATGTACTCACCCAGACGATCAAACAACGCATACAACGCG   >  1:1909685/1‑100
                                                                                                      CGCAATCCCACCCAGATACGCGAAAATTACGCCGCCGTGAGTATTCAGGCCAATGAATGGAATGTACTCACCCAGACGATCAAACAACGCATACAACGCG   >  2:1052789/1‑100
                                                                                                      CGCAATCCCACCCAGATACGCGAAAATTACGCCGCCGTGAGTATTCAGGCCAATGAATGGAATGTACTCACCCAGACGATCAAACAACGCATACAACGCG   >  2:1209488/1‑100
                                                                                                       GCAATCCCACCCAGATACGCGAAAATTACGCCGCCGTGAGTATTCAGGCCAATGAATGGAATGTACTCACCCAGACGATCAAACAACGCATACAACGCGA  <  2:749588/100‑1
                                                                                                                                                                                                           
AATGGCAGCGATAAACGACAGGATGAATACCACCGCCAGAATCGGTACTGACAGCGGCAACAGGACAAGGCGGAAGGCCTGCCACGGTGTCGCACCATCCAGCGCA‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑  >  NC_000913/4242836‑4242941
‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑cagcgcaATCCCACCCAGATACGCGAAAATTACGCCGCCGTGAGTATTCAGGCCAATGAATGGAATGTACTCACCCAGACGATCAAACAACGCATACAACGCGA  >  NC_000913/4243008‑4243104

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑
Reads not counted as support for junction
read_name Not counted due to insufficient overlap past the breakpoint.
read_name Not counted due to not crossing MOB target site duplication.

GATK/CNVnator alignment

N/A