Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A12 F56 I0 R1 312 170.3 8276235 93.7% 7754832 99.8

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 3,971,690 A→C 100% Q144P (CAG→CCG)  wecC → UDP‑N‑acetyl‑D‑mannosaminuronic acid dehydrogenase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,971,6900AC99.4% 536.2 / ‑5.1 154Q144P (CAG→CCG) wecCUDP‑N‑acetyl‑D‑mannosaminuronic acid dehydrogenase
Reads supporting (aligned to +/- strand):  ref base A (0/1);  new base C (73/80);  total (73/81)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.79e-01

Only 100 of 162 total aligned reads displayed.

GCGCGCTGGTGATCCTTGAATCCACCTCGCCGGTGGGGTCAACCGAGAAGATGGCAGAATGGTTAGCAGAGATGCGTCCGGATCTCACTTTCCCGCAGCAGGTGGGCGAGCAGGCGGACGTCAACATTGCTTACTGCCCGGAACGCGTGTTACCAGGACAGGTAATGGTCGAGCTGATTAAAAACGATCGCG  >  NC_000913/3971594‑3971785
                                                                                                |                                                                                               
 cgcgcTGGTGATCCTTGAATCCACCTCGCCGGTGGGGTCAACCGAGAAGATGGGGGGGGGGTTAGCAGAGATGCGTCCGGATCTCACTTTCTCGccgcag                                                                                             <  1:1164086/100‑1 (MQ=255)
  gcgcTGGTGATCCTTGAATCCACCTCGCCGGTGGGGTCAACCGAGAAGATGGCAGAATGGTTAGCAGAGATGCGTCCGGATCTCACTTTCCCGCCGCAgg                                                                                            <  2:1237491/100‑1 (MQ=255)
   cgttGGTGATCCTTGAATCCACCTCGCCGGTGGGGTCAACCGAGAAGATGGCAGAATGGTTAGCAGAGATGCGTCCGGATCTCACTTTCCCGCCGCAGGt                                                                                           <  2:2315079/97‑1 (MQ=255)
       ggTGATCCTTGAATCCACCTCGCCGGTGGGGTCAACCGAGAAGATGGCAGAATGGTTAGCAGAGATGCGTCCGGATCTCACTTTCCCGCCGCAGGTGGGc                                                                                       >  1:3768332/1‑100 (MQ=255)
        gTGATCCTTGAATCCACCTCGCCGGTGGGGTCAACCGAGAAGATGGCAGAATGGTTAGCAGAGATGCGTCCGGATCTCACTTTCCCGCCGCAGGTGGGc                                                                                       <  1:2539866/99‑1 (MQ=255)
         tGATCCTTGAATCCACCTCGCCGGTGGGGTCAATCGAGCAGATGGCAGAATGGGTAGCCGGGATGCGGCCGGATTTCACGTTCCCGGCGCCGGTGTGc                                                                                       >  2:829940/1‑98 (MQ=255)
         tGATCCTTGAATCCACCTCGCCGGTGGGGTCAACCGAGAAGATGGCAGAATGGTTAGCCGAGATGCGTCCGGATCTCACTTCCCCGCCGAAGGTGGGCGa                                                                                     >  2:2561277/1‑100 (MQ=255)
          gATCCTTGAATCCACCTCGCCGGTGGGGTCAACCGAGAAGATGGCAGAATGGTTAGCAGAGATGCGTCCGGATCTCACTTTCCCGCCGCAGGTGGGCGAg                                                                                    >  1:3536344/1‑100 (MQ=255)
            tCCTTGAATCCACCTCGCCGGTGGGGTCAACCGAGAAGATGGCAGAATGGTTAGCAGAGATGCGTCCGGATCTCACTTTCCCGCCGCAGGTGGGCGAGCa                                                                                  >  1:567218/1‑100 (MQ=255)
            tCCTTGAATCCACCTCGCCGGTGGGGTCAACCGAGAAGATGGCAGAATGGTTAGCAGAGATGCGTCCGGATCTCACTTTCCCGCCGCAGGTGGGCGAGCa                                                                                  >  2:54936/1‑100 (MQ=255)
              cTTGAATCCACCTCGCCGGTGGGGTCAACCGAGAAGATGGCAGAATGGTTAGCAGAGATGCGTCCGGATCTCACTTTCCCGCCGCAGGTGGGCGAGCAgg                                                                                >  1:1688776/1‑100 (MQ=255)
              cTTGAATCCACCTCGCCGGTGGGGTCAACCGAGAAGATGGCAGAATGGTTAGCAGAGATGCGTCCGGATCTCACTTTCCCGCCGCAGGTGGGCGAGCAgg                                                                                >  1:3350009/1‑100 (MQ=255)
              cTTGAATCCACCTCGCCGGTGGGGTCAACCGAGAAGATGGCAGAATGGTTAGCAGAGATGCGTCCGGATCTCACTTTCCCGCCGCAGGTGGGCGAGCAgg                                                                                >  1:3492488/1‑100 (MQ=255)
               ttGAATCCACCTCGGCGGTGGGGTCAACCGAGATGATGGCCGAATGTTGGGGAGAGATGCGGCCGGAACGTAGGTTAACGCCGCAGGGGGGCGAGAAggg                                                                               >  2:3563824/1‑99 (MQ=255)
               ttGAATCCACCTCGCCGGTGGGGTCAACCGAGAAGATGGCAGAATGGTTAGCAGAGATGCGTCCGGATCTCACTTTCCCGCCGCAGGTGGGCGAGCAGGc                                                                               <  1:2614258/100‑1 (MQ=255)
                 gAATCCACCTCGCCGGTGGGGTCAACCGAGAAGATGGCAGAATGGTTAGCAGAGATGCGTCCGGATCTCACTTTCCCGCCGCAGGTGGGCGAGCAGGCgg                                                                             <  1:1571478/100‑1 (MQ=255)
                 gAATCCACCTCGCCGGTGGGGTCAACCGAGAAGATGGCAGAATGGTTAGCAGAGATGCGTCCGGATCTCACTTTCCCGCCGCAGGTGGGCGAGCAGGCgg                                                                             <  2:2082368/100‑1 (MQ=255)
                  aaTCCACCTCGCCGGTGGGGTCAACCGAGAAGATGGCAGAATGGTTAGCAGAGATGCGTCCGGATCTCACTTTCCCGCCGCAGGTGGGCGAGCAGGCGGa                                                                            <  2:913856/100‑1 (MQ=255)
                   aTCCACCTCGCCGGTGGGGTCAACCGAGAAGATGGCAGAATGGTTAGCAGAGATGCGTCCGGATCTCACTTTCCCGCCGCAGGTGGGCGAGCAGGCGGAc                                                                           >  1:3534571/1‑100 (MQ=255)
                   aTCCACCTCGCCGGTGGGGTCAACCGAGAAGATGGCAGAATGGTTAGCAGAGATGCGTCCGGATCTCACTTTCCCGCCGCAGGTGGGCGAGCAGGCGGAc                                                                           >  1:1800808/1‑100 (MQ=255)
                    tCCACCTCGCCGGTGGGGTCAACCGAGAAGATGGCAGAATGGTTAGCAGAGATGCGTCCGGATCTCACTTTCCCGCCGCAGGTGGGCGAGCAGGCGGACg                                                                          >  1:649614/1‑100 (MQ=255)
                    tCCACCTCGCCGGTGGGGTCAACCGAGAAGATGGCAGAATGGTTAGCAGAGATGCGTCCGGATCTCACTTTCCCGCCGCAGGTGGGCGAGCAGGCGGACg                                                                          <  2:3494209/100‑1 (MQ=255)
                    tCCACCTCGCCGGTGGGGTCAACCGAGAAGATGGCAGAATGGTTAGCAGAGATGCGTCCGGATCTCACTTTCCCGCCGCAGGTGGGCGAGCAGGCGGACg                                                                          <  2:1027601/100‑1 (MQ=255)
                     ccACCTCGCCGGTGGGGTCAACCGAGAAGATGGCAGAATGGTTAGCAGAGATGCGTCCGGATCTCACTTTCCCGCCGCAGGTGGGCGAGCAGGCGGACGt                                                                         <  1:1529432/100‑1 (MQ=255)
                     ccACCTCGCCGGTGGGGTCAACCGAGAAGATGGCAGAATGGTTAGCAGAGATGCCTCCGGATCTCACATTCCCGCCGCAGGTGGGCGAGCAGGCGGCCGt                                                                         >  2:418621/1‑100 (MQ=255)
                      cACCTCGCCGGTGGGGTCAACCGAGAAGATGGCAGAATGGTTAGCAGAGATGCGTCCGGATCTCACTTTCCCGCCGCAGGTGGGCGAGCAGGCGGACGTc                                                                        <  1:3138252/100‑1 (MQ=255)
                       aCCTCGCCGGTGGGGTCAACCGAGAAGATGGCAGAATGGTTAGCAGAGATGCGTCCGGATCTCACTTTCCCGCCGCAGGTGGGCGAGCAGGCGGACGTCa                                                                       >  1:1199872/1‑100 (MQ=255)
                         cTCGCCGGTGGGGTCAACCGAGAAGATGGCAGAAGGGTTAGCAGAGATGCGTCCGGATCTCACTTTCCCGCCGCAGGTGGGCGAGCAGGCGGACGTCAAc                                                                     <  1:996175/100‑1 (MQ=255)
                          tCGCCGGTGGGGTCAACCGAGAAGATGGCAGAATGGTTAGCAGAGATGCGTCCGGATCTCACTTTCCCGCCGCAGGTGGGCGAGCAGGCGGACGTCAACa                                                                    <  1:2845961/100‑1 (MQ=255)
                             ccGGTGGGGTCAACCGAGAAGATGGCAGAATGGTTAGCAGAGATGCGTCCGGATCTCACTTTCCCGCCGCAGGTGGGCGAGCAGGCGGACGTCAACATTg                                                                 >  1:433056/1‑100 (MQ=255)
                               ggTGGGGTCAACCGAGAAGATGGCAGAATGGTTAGCAGAGATGCGTCCGGATCTCACTTTCCCGCCGCAGGTGGGCGAGCAGGCGGACGTCAACATTGCt                                                               <  2:519000/100‑1 (MQ=255)
                                 tGGGGTCAACCGAGAAGATGGCAGAATGGTTAGCAGAGATGCGTCCGGATCTCACTTTCCCGCCGCAGGTGGGCGAGCAGGCGGACGTCAACATTGCTTa                                                             <  1:479876/100‑1 (MQ=255)
                                  ggggTCAACCGAGAAGATGGCAGAATGGTTAGCAGAGATGCGTCCGGATCTCACTTTCCCGCCGCAGGTGGGCGAGCAGGCGGACGTCAACATTGCTTAc                                                            <  2:3611109/100‑1 (MQ=255)
                                   nggTCAACCGAGAAGATGGCAGAATGGTTAGCAGAGATGCGTCCGGATCTCACTTTCCCGCCGCAGGTGGGCGAGCAGGCGGACGTCAACATTGCTTACt                                                           >  1:3142224/2‑100 (MQ=255)
                                    ggTCAACCGAGAAGATGGCAGAATGGTTAGCAGAGATGCGTCCGGATCTCACTTTCCCGCCGCAGGTGGGCGAGCAGGCGGACGTCAACATTGCTTACTg                                                          >  1:3261047/1‑100 (MQ=255)
                                    ggTCAACCGAGAAGATGGCAGAATGGTTAGCAGAGATGCGTCCGGATCTCACTTTCCCGCCGCAGGTGGGCGAGCAGGCGGACGTCAACATTGCTTACTg                                                          <  2:2954298/100‑1 (MQ=255)
                                    ggTCAACCGAGAAGATGGCAGAATGGTTAGCAGAGATGCGTCCGGATCTCACTTTCCCGCCGCAGGAAGGCGAGTAGGCGGACGTCAACATTGCTTAc                                                            >  2:3797176/1‑98 (MQ=255)
                                     gTCAACCGAGAAGATGGCAGAATGGTTAGCAGAGATGCGTCCGGATCTCACTTTCCCGCCGCAGGTGGGCGAGCAGGCGGACGTCAACATTGCTTACTGc                                                         >  1:1280081/1‑100 (MQ=255)
                                     gTCAACCGAGAAGATGGCAGAATGGTTAGCAGAGATGCGTCCGGATCTCACTTTCCCGCCGCAGGTGGGCGAGCAGGCGGACGTCAACATTGCTTACTGc                                                         <  2:2090927/100‑1 (MQ=255)
                                       cAACCGAGAAGATGGCAGCATGGTTAGCAGAGATGCGTCCGGCTCTCACTTTCACGCCGCAGGTGGGCGAGCAGGCGGACGTCAACATTGCTTACTGccc                                                       >  2:49035/1‑100 (MQ=255)
                                        aaCCGAGAAGATGGCAGAATGGTTAGCAGAGATGCGTCCGGATCTCACTTTCCCGCCGCAGGTGGGCGAGCAGGCGGACGTCAACATTGCTTACTGCCCg                                                      <  2:891931/100‑1 (MQ=255)
                                        aaCCGAGAAGATGGCAGAATGGTTAGCAGAGATGCGTCCGGATCTCACTTTCCCGCCGCAGGTGGGCGAGCAGGCGGACGTCAACATTGCTTACTGCCCg                                                      <  1:480856/100‑1 (MQ=255)
                                        aaCCGAGAAGATGGCAGAATGGTTAGCAGAGATGCGTCCGGATCTCACTTTCCCGCCGCAGGTGGGCGAGCAGGCGGACGTCAACATTGCTTACTGCCCg                                                      <  1:732310/100‑1 (MQ=255)
                                         aCCGAGAAGATGGCAGAATGGTTAGCAGAGATGCGTCCGGATCTCACTTTCCCGCCGCAGGTGGGCGAGCAGGCGGACGTCAACATTGCTTACTGCCCgg                                                     >  1:407652/1‑100 (MQ=255)
                                          ccGAGAAGATGGCAGAATGGTTAGCAGAGATGCGTCCGGATCTCACTTTCCCGCCGCAGGTGGGCGAGCAGGCGGACGTCAACATTGCTTACTGCCCGGa                                                    >  1:4122690/1‑100 (MQ=255)
                                            gagaAGATGGCAGAATGGTTAGCAGAGATGCGTCCGGATCTCACTTTCCCGCCGCAGGTGGGCGAGCAGGCGGACGTCAACATTGCTTACTGCCCGGAAc                                                  >  1:2725550/1‑100 (MQ=255)
                                            gagaAGATGGCAGAATGGTTAGCAGAGATGCGTCCGGATCTCACTTTCCCGCCGCAGGTGGGCGAGCAGGCGGACGTCAACATTGCTTACTGCCCGGAAc                                                  <  1:3340465/100‑1 (MQ=255)
                                               aagaTGGCAGAATTGTTAGCAGAGATGCGTCCGGATCTCACTTTCCCGCCGCAGGTGGGCGAGCAGGCGGACGTCAACATTGCTTACTGCCCGGAAcgcg                                               <  1:2401075/100‑1 (MQ=255)
                                               aagaTGGCAGAATGGTTAGCAGAGATGCGTCCGGATCTCACTTTCCCGCCGCAGGTGGGCGAGCAGGCGGACGTCAACATTGCTTACTGCCCGGAAcgcg                                               <  2:1508867/100‑1 (MQ=255)
                                               aagaTGGCAGAATGGTTAGCAGAGATGCGTCCGGATCTCACTTTCCCGCCGCAGGTGGGCGAGCAGGCGGACGTCAACATTGCTTACTGCCCGGAAcgcg                                               <  2:2469722/100‑1 (MQ=255)
                                                agaTGGCAGAATGGTTAGCAGAGATGCGTCCGGATCTCACTTTCCCGCCGCAGGTGGGCGAGCAGGCGGACGTCAACATTGCTTACTGCCCGGAACGCgt                                              >  2:3350993/1‑100 (MQ=255)
                                                 gagggCAGAATGGTTAGCAGAGATGCGTCCGGATCTCACTTTCCCGCCGCAGGTGGGCGAGCAGGCGGACGTCAACATTGCTTACTGCCCGGAACGCgtn                                             <  2:3947898/97‑2 (MQ=255)
                                                 gaTGGCAGAATGGTTAGCAGAGATGCGTCCGGATCTCACTTTCCCGCCGCAGGTGGGCGAGCAGGCGGACGTCAACATTGCTTACTGCCCGGAACGCgtg                                             <  2:893821/100‑1 (MQ=255)
                                                 gaTGGCAGAATGGTTAGCAGAGATGCGTCCGGATCTCACTTTCCCGCCGCAGGTGGGCGAGCAGGCGGACGTCAACATTGCTTACTGCCCGGAACGCgtg                                             <  1:368553/100‑1 (MQ=255)
                                                 gaTGGCAGAATGGTTAGCAGAGATGCGTCCGGATCTCACTTTCCCGCCGCAGGTGGGCGAGCAGGCGGACGTCAACATTGCTTACTGCCCGGAACGCgtg                                             <  2:1328512/100‑1 (MQ=255)
                                                 gaTGGCAGAATGGTTAGCAGAGATGCGTCCGGATCTCACTTTCCCGCCGCAGGTGGGCGAGCAGGCGGACGTCAACATTGCTTACTGCCCGGAACGCgtg                                             <  2:424867/100‑1 (MQ=255)
                                                      cAGAATGGTTAGCAGAGATGCGTCCGGATCTCACTTTCCCGCCGCAGGTGGGCGAGCAGGCGGACGTCAACATTGCTTACTGCCCGGAACGCGTGTTAcc                                        >  2:520915/1‑100 (MQ=255)
                                                      cAGAATGGTTAGCAGAGATGCGTCCGGATCTCACTTTCCCGCCGCAGGTGGGCGAGCAGGCGGACGTCAACATTGCTTACTGCCCGGAACGCGTGTTAcc                                        <  1:929496/100‑1 (MQ=255)
                                                      cAGAATGGTTAGCAGAGATGCGTCCGGATCTCACTTTCCCGCCGCAGGTGGGCGAGCAGGCGGACGTCAACATTGCTTACTGCCCGGAACGCGTGTTAcc                                        <  1:414008/100‑1 (MQ=255)
                                                      cAGAATGGTTAGCAGAGATGCGTCCGGATCTCACTTTCCCGCCGCAGGTGGGCGAGCAGGCGGACGTCAACATTGCTTACTGCCCGGAACGCGTGTTAcc                                        >  1:3743493/1‑100 (MQ=255)
                                                       aGAATGGTTAGCAGAGATGCGTCCGGATCTCACTTTCCCGCAGCAGGTGGGCGAGCAGGCGGACGTCAACATTGCTTACTGCCCGGAACGCGTGTTACCa                                       <  1:1592350/100‑1 (MQ=255)
                                                            ggTTAGCATAGATGCGTCCGGATCTCACTTACCCGCCGCAGGTGGGCGAGCATGCGGACGTCAACATTGCTTACTGCCCGGAACGCGTATTACCAGGACa                                  <  2:272862/100‑1 (MQ=255)
                                                            ggTTAGCAGAGATGCGTCCGGATCTCACTTTCCCGCCGCAGGTGGGCGAGCAGGCGGACGTCAACATTGCTTACTGCCCGGAACGCGTGTTACCAGGACa                                  <  1:3785133/100‑1 (MQ=255)
                                                            ggTTAGCAGAGATGCGTCCGGATCTCACTTTCCCGCCGCAGGTGGGCGAGCAGGCGGACGTCAACATTGCTTACTGCCCGGAACGCGTGTTACCAGGACa                                  <  1:804807/100‑1 (MQ=255)
                                                             gTTAGCAGAGATGCGTCCGGATCTCACTTTCCCGCCGCAGGTGGGCGAGCAGGCGGACGTCAACATTGCTTACTGCCCGGAACGCGTGTTACCAGGACAg                                 <  1:3316809/100‑1 (MQ=255)
                                                              ttAGCAGAGATGCGTCCGGATCTCACTTTCCCGCCGCAGGTGGGCGAGCAGGCGGACGTCAACATTGCTTACTGCCCGGAACGCGTGTTACCAGGACAgg                                >  1:1433380/1‑100 (MQ=255)
                                                              ttAGCAGAGATGCGTCCGGATCTCACTTTCCCGCCGCAGGTGGGCGAGCAGGCGGACGTCAACATTGCTTACTGCCCGGAACGCGTGTTACCAGGACAgg                                <  2:3737338/100‑1 (MQ=255)
                                                              ttAGCAGAGATGCGTCCGGATCGCACTTTCCCGCCGCAGGTGGGCGAGCAGGCGGACGTCAACATTGCTTACTGCCCGGAACGCGTGTTACCAGGACAgg                                <  2:1783421/100‑1 (MQ=255)
                                                               tAGCAGAGATGCGTCCGGATCTCACTTTCCCGCCGCAGGTGGGCGAGCAGGCGGACGTCAACATTGCTTACTGCCCGGAACGCGTGTTACCAGGACAGGt                               >  2:3115207/1‑100 (MQ=255)
                                                                   agagATGCGTCCGGATCTCACTTTCCCGCCGCAGGTGGGCGAGCAGGCGGACGTCAACATTGCTTACTGCCCGGAACGCGTGTTACCAGGACAGGTAAt                            <  2:1947310/99‑1 (MQ=255)
                                                                   agagATGCGTCCGGATCTCACTTTCCCGCCGCAGGTGGGCGAGCAGGCGGACGTCAACATTGCTTACTGCCCGGAACGCGTGTTACCAGGACAGGTAATg                           >  2:2892003/1‑100 (MQ=255)
                                                                   agagATGCGTCCGGATCTCACTTTCCCGCCGCAGGTGGGCGAGCAGGCGGACGTCAACATTGCTTACTGCCCGGAACGCGTGTTACCAGGACAGGTAATg                           >  2:3070785/1‑100 (MQ=255)
                                                                    gagaTGCGTCCGGATCTCACTTTCCCGCCGCAGGTGGGCGAGCAGGCGGACGTCAACATTGCTTACTGCCCGGAACGCGTGTTACCAGGACAGGTAATgg                          <  1:2307812/100‑1 (MQ=255)
                                                                    gagaTGCGTCCGGATCTCACTTTCCCGCCGCAGGTGGGCGAGCAGGCGGACGTCAACATTGCTTACTGCCCGGAACGCGTGTTACCAGGACAGGTAATgg                          >  1:2278200/1‑100 (MQ=255)
                                                                    gagaTGCGTCCGGATCTCACTTTCCCGCCGCAGGTGGGCGAGCAGGCGGACGTCAACATTGCTTACTGCCCGGAACGCGTGTTACCAGGACAGGTAATgg                          <  1:950970/100‑1 (MQ=255)
                                                                        tGCGTCCGGATCTCACTTTCCCGCCGCAGGTGGGCGAGCAGGCGGACGTCAACATTGCTTACTGCCCGGAACGCGTGTTACCAGGACAGGTAATGGTCGa                      >  1:3854123/1‑100 (MQ=255)
                                                                        tGCGTCCGGATCTCACTTTCCCGCCGCAGGTGGGCGAGCAGGCGGACGTCAACATTGCTTACTGCCCGGAACGCGTGTTACCAGGACAGGTAATGGTCGa                      >  1:1115970/1‑100 (MQ=255)
                                                                         gCGTCCGGATCTCACTTTCCCGCCGCAGGTGGGCGAGCAGGCGGACGTCAACATTGCTTACTGCCCGGAACGCGTGTTACCAGGACAGGTAATGGTCGAg                     <  1:1962962/100‑1 (MQ=255)
                                                                         gCGTCCGGATCTCACTTTCCCGCCGCAGGTGGGCGAGCAGGCGGACGTCAACATTGCTTACTGCCCGGAACGCGTGTTACCAGGACAGGTAATGGTCGAg                     <  2:534462/100‑1 (MQ=255)
                                                                          cGTCCGGATCTCACTTTCCCGCCGCAGGTGGGCGAGCAGGCGGACGTCAACATTGCTTACTGCCCGGAACGCGTGTTACCAGGACCGGTAATGGTCGAGc                    >  2:1000162/1‑100 (MQ=255)
                                                                           gTCCGGATCTCACTTTCCCGCCGCAGGTGGGCGAGCAGGCGGCCGTCTACATTGCTTACTGCCCGGAACGCGTGTTACCAGGACAGGTAATGGGCGAGCt                   >  2:3719424/1‑100 (MQ=255)
                                                                           gTCCGGATCTCACTTTCCCGCCGCAGGTGGGCGAGCAGGCGGACGTCAACATTGCTTACTGCCCGGAACGCGTGTTACCAGGACAGGTAATGGTCGAGCt                   <  1:3539395/100‑1 (MQ=255)
                                                                             ccGGATTTCACGTTTCCGCCGCAGGTGGGGGGGCAGGCGGGCGTCAACATTGCTTCCTGCCCGGAACGCGTGTTACCAGGACGGGTAATGGTCGGGCTg                  >  2:383435/1‑99 (MQ=255)
                                                                             ccGGATCTCACTTTCCCGCCGCAGGTGGGCGAGCAGGCGGACGTCAACATTGCTTACTGCCCGGAACGCGTGTTACCAGGACAGGTAATGGTCGAGCTGa                 <  2:3836467/100‑1 (MQ=255)
                                                                              cGGATCTCACTTTCCCGCCGCAGGTGGGCGAGCAGGCGGACGTCAACATTGCTTACTGCCCGGAACGCGTGTTACCAGGACAGGTAATGGTCGAGCTGAt                <  2:1953181/100‑1 (MQ=255)
                                                                              cGGATCTCACTTTCCCGCCGCAGGTGGGCGAGCAGGCGGACGTCAACATTGCTTACTGCCCGGAACGCGTGTTACCAGGACAGGTAATGGTCGAGCTGAt                <  1:1004343/100‑1 (MQ=255)
                                                                               ggATCTCACTTTCCCGCCGCAGGTGGGCGAGCAGGCGGACGTCAACATTGCTTACTGCCCGGAACGCGTGTTACCAGGACAGGTAATGGTCGAGCTGAtt               <  2:3204267/100‑1 (MQ=255)
                                                                               ggATCTCACTTTCCCGCCGCAGGTGGGCGAGCAGGCGGACGTCAACATTGCTTACTGCCCGGAACGCGTGTTACCAGGACAGGTAATGGTCGAGCTGAtt               >  1:3405883/1‑100 (MQ=255)
                                                                               ggATCTCACTTTCCCGCCGCAGGGGGGCGAGCAGGCGGACGTCAACATTGCTTACTGCCCGCAACGCGTGTTACCAGGACAGGTAATGGTCGAGCTGAt                <  2:2214982/99‑1 (MQ=255)
                                                                                gATCTCACTTTCCCGCCGCAGGTGGGCGAGCAGGCGGACGTCAACATTGCTTACTGCCAGGAACGCGTGTTACCAGGACAGGTAATGGTCGAGCTGATTa              <  2:2258034/100‑1 (MQ=255)
                                                                                  nctcACTTTCCCGCCGCAGGTGGGCGAGCAGGCGGACGTCAACATTGCTTACTGCCCGGAACGCGTGTTACCAGGACAGGTAATGGTCGAGCTGATTaaa            >  2:2803760/2‑100 (MQ=255)
                                                                                   ctcACTTTCCCGCCGCAGGTGGGCGAGCAGGCGGACGTCAACATTGCTTACTGCCCGGAACGCGTGTTACCAGGACAGGTAATGGTCGAGCTGATTaaaa           <  1:437934/100‑1 (MQ=255)
                                                                                    tcACTTTCCCGCCGTAGGTGGGCTTGGAGGCGGACGTCGACAGTGCTTATTGCCCGGAACGCGTGTTTCCAGTACAGTTAATTGTCGAGCTGATTtaat           <  2:16363/99‑2 (MQ=255)
                                                                                    tcACTTCCCCGCCGCAGGTGGGCGCGCAGGCGGACGTCAACATTGCTTCCTGCCCGGAACGCGTGTTACCAGGACAGGAAATGGTCGAGCTGATTaaa            >  2:2913289/1‑98 (MQ=255)
                                                                                      aCTTTCCCGCCGCAGGTGGGCGAGCAGGCGGACGTCAACATTGCTTACTGCCCGGAACGCGTGTTACCAGGACAGGTAATGGTCGAGCTGATTaaaaa          >  2:2454323/1‑98 (MQ=255)
                                                                                      aCTTTCCCGCCGCAGGTGGGCGAGCAGGCGGACGTCAACATTGCTTACTGCCCGGAACGCGTGTTACCAGGACAGGTAATGGTCGAGCTGATTAAAAACg        >  1:2925533/1‑100 (MQ=255)
                                                                                      aCTTTCCCGCCGCAGGTGGGCGAGCAGGCGGACGTCAACATTGCTTACTGCCCGGAACGCGTGTTACCAGGACAGGTAATGGTCGAGCTGATTAAAAACg        <  2:2092164/100‑1 (MQ=255)
                                                                                        tttCCCGCCGCAGGTGGGCGAGCAGGCGGACGTCAACATTGCTTACTGCCCGGAACGCGTGTTACCAGGACAGGTAATGGTCGAGCTGATTAAAAACGAt      >  1:1869134/1‑100 (MQ=255)
                                                                                           cccGCCGCAGGTGGGCGAGCAGGCGGACGTCAACATTGCTTACTGCCCGGAACGCGTGTTACCAGGACAGGTAATGGTCGAGCTGATTAAAAACGATcgc   >  1:3790973/1‑100 (MQ=255)
                                                                                           cccGCCGCAGGTGGGCGAGCAGGCGGACGTCAACATTGCTTACTGCCCGAAACGCGTGTTACCAGGACAGGTAATGGTCGAGCTGATTAAAAACGATcgc   <  1:3718690/100‑1 (MQ=255)
                                                                                                |                                                                                               
GCGCGCTGGTGATCCTTGAATCCACCTCGCCGGTGGGGTCAACCGAGAAGATGGCAGAATGGTTAGCAGAGATGCGTCCGGATCTCACTTTCCCGCAGCAGGTGGGCGAGCAGGCGGACGTCAACATTGCTTACTGCCCGGAACGCGTGTTACCAGGACAGGTAATGGTCGAGCTGATTAAAAACGATCGCG  >  NC_000913/3971594‑3971785

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A